JP2003040757A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin care preparation containing Ganoderma atrum extract. SOLUTION: This skin care preparation contains Ganoderma atrum extract which has both especially high melanogenesis inhibitoty effect and histamine liberation inhibitory effect compared to Ganoderma lucidum extract and high stability as well. The other version of this skin care preparation contains a combination of the Ganoderma atrum extract with the Ganoderma lucidum extract, exhibiting synergistic effect, and also being safe and exhibiting aging inhibitoty, bleaching and anti-inflammatory effects.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a skin external preparation which is particularly excellent in whitening, anti-inflammatory and anti-aging effects by containing an extract of Black Reishi.

[0002]

BACKGROUND OF THE INVENTION Skin pigmentation, which is commonly seen in spots, freckles, and sunburn, is caused by melanin pigment-producing cells in the skin that produce excessive melanin pigment due to hormonal abnormalities and ultraviolet ray stimulation. It is thought that the cause is the deposition inside. As one of methods for preventing such pigmentation, a method for suppressing excessive production of melanin is known.

As one of the causes of rough skin, there is skin inflammation due to dryness or ultraviolet rays. Due to these inflammations, the firmness of the skin disappears and it may cause wrinkles and tarmi. Therefore, a skin external preparation that suppresses inflammation and improves rough skin is desired.

Conventionally, such rough skin, stains, freckles,
As a cosmetic effective for improving firmness, wrinkles, tarmi and the like, cosmetics containing an extract of Ganoderma lucidum (Ganoderma lucidum) as disclosed in JP-A-58-113118 and JP-A-05-194175 have been known. Ganoderma lucidum used in these cosmetics is the herbal medicine "Reishi"
Basidiomycetes used for
dermataceae), Ganoderma genus (Gano)
Derma), Reishi (red turf), Black Reishi (Black turf),
Including Shireishi (Shishiba) and the like. However, in these publications,
Only used as Ganoderma lucidum, Reishi, Black Reishi,
Shireishiba was not compared in detail.

Among Ganoderma lucidum, Reishi, Black Reishi, Purple Reishi, etc., are simply named because their fruit bodies are red, black, and purple, but recently, black Research is being conducted by distinguishing Reishi from Reishi and Purple Reishi. For example, reference [Journal of Japan Brewery, 85 (6), 385-392, 19
90], it is reported that black Reishi does not contain the typical triterpene components contained in Reishi and purple Reishi, and the taste is completely different. From the above, there was room for further research into the effects of Reishi, Black Reishi, and Purple Reishi on the skin.

From the above, there is a demand for a skin external preparation which is safe and excellent in stability, and which is excellent in whitening, anti-inflammatory and antiaging effects.

[0006]

Under these circumstances, the inventors of the present invention have diligently studied and studied, and as a result, among Ganoderma lucidum, the extract of Black Reishi has a particularly excellent melanin production inhibitory effect and histamine release inhibitory effect. It was also found that it is also excellent in stability. Furthermore, it was found that the combined use of black reishi and reishi (red shiba) results in a synergistic effect rather than the use of black reishi alone. Also,
The inventors have found that an external preparation for skin containing these extracts is safe and stable and has excellent whitening, anti-inflammatory and anti-aging effects, and completed the present invention.

Black reishi (black turf) and reishi (red turf) used in the present invention are basidiomycetes used for the herbal medicine "Reishi".
It is a kind of Ganoderma lucidum that belongs to Ganoderma lucidum and Ganoderma genus. Its scientific name is [Acta Micr].
obilogica Sinica, 19 (3), 2
65-279, 1979], Black Reishi (Kuroshiba, Scientific name: Ganoderma atrum), Reishi (Red Shiba,
Scientific name: Ganoderma lucidum), but the classification method is not limited to this document. As the black reishi and the reishi (red turf) used in the present invention, those widely distributed in the Chinese and Japanese markets can be used.

The extract of black reishi or reishi (red shiba) used in the present invention can be extracted from fruiting bodies, mycelia, cultures of mycelia and the like. Extracts from fruit bodies are preferable. The extraction method is not particularly limited, and may be, for example, heat extraction or normal temperature extraction. The mycelium or a culture of the mycelium can be produced by a conventional method.

The solvent to be extracted is, for example, water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol (1,3-butylene glycol). , Propylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran) , Propyl ether, etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

The above-mentioned extract may be used as it is as an extracted solution, or may be subjected to treatment such as concentration, dilution, filtration, decolorization with activated carbon, deodorization and the like, if necessary. Furthermore,
The extracted solution may be concentrated to dryness, spray-dried, freeze-dried or the like and used as a dried product, or may be subjected to column purification or the like to concentrate or isolate the active ingredient.

For the external preparation for skin of the present invention, the above extract may be used as it is, and as long as the effect of the extract is not impaired, oils and fats, waxes, which are components used in the external preparation,
Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants,
Ingredients such as a whitening agent and a chelating agent may be added.

The external preparation for skin of the present invention can be used in any of cosmetics, quasi-drugs and pharmaceuticals, and its dosage forms include, for example, lotions, creams, emulsions, gels,
Aerosol agent, essence, pack, cleaning agent, bath agent,
The thing applied to the skin, such as foundation, dusting powder, lipstick, ointment, and poultice, is mentioned.

The amount of the above extract used in the present invention is 0.0 based on the total amount of the external preparation for skin of the present invention in terms of solid matter.
001% by weight or more, preferably 0.001 to 10% by weight
Is a good combination. If it is less than 0.0001% by weight, it is difficult to expect a sufficient effect. If the amount is more than 10% by weight, it is uneconomical to enhance the effect. Regarding the method of addition, it may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

Among the extracts used in the present invention, the combined use of the extracts of black reishi and reishi (red shiba) exhibits synergistic whitening, anti-inflammatory and anti-aging effects on the skin.
Further, the compounding ratio of the extract of black reishi and reishi (red shiba) is 5:95 to 95: 5, preferably 10: 9, by weight of solids.
It is 0 to 90:10.

[0015]

EXAMPLES In order to explain the present invention in detail, production examples of the extract used in the present invention, prescription examples of the present invention and experimental examples will be given below, but the present invention is not limited thereto. Absent. The parts of the compounding amounts shown in the examples are parts by weight, and% means% by weight.

Production Example 1 Hot-Water Extract of Black Reishi Maturation 20 mL of dried Black Reishi fruiting body was added with 400 mL of purified water, extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated. Freeze-dry the hot water extract of Black Reishi to 1.
4 g was obtained.

Production Example 2 Ethanol Extract of Black Reishi Grass To 100 g of dried Black Reishi grass, 2 L of ethanol was added, and the mixture was extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. 1.6 g of ethanol extract was obtained.

Production Example 3 Extraction of 50% 1,3-butylene glycol aqueous solution of Black Ganoderma lucidum To 100 g of dried Black Ganoderma lucidum, 1 L of purified water and 1 L of 1,3-butylene glycol were added, and the mixture was extracted at room temperature for 7 days. ,
After filtration, 1.9 kg of a 50% 1,3-butylene glycol aqueous solution extract of Black Reishi was obtained.

Production Example 4 Hot Water Extract of Reishi (Red Shiba) 400 mL of purified water was added to 20 g of dried dry Reishi (Red Shiba), and the mixture was extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated. Then, it was freeze-dried to obtain 2.0 g of a hot water extract of Reishi (Akashiba).

Production Example 5 Ethanol Extract of Reishi (Red Shiba) 2 L of ethanol was added to 100 g of dried product of Reishi (Red Shiba), and the mixture was extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. , 2.7 g of ethanol extract of Reishi (Akashiba)
Obtained.

Production Example 6 50% of Reishi (Akashiba) 1,3-
Butylene glycol aqueous solution extract Purified water 1L and 1,3 to 100g dry matter of Reishi (Akashiba)
1 L of butylene glycol was added, and the mixture was extracted at room temperature for 7 days and then filtered to obtain 1.9 kg of a 50% 1,3-butylene glycol aqueous solution extract of Reishi (red turf).

Production Example 7 Extract of Mycelia mycelia culture of Black Reishi cultivated using a jar fermenter. That is, 50 g malt extract, 20 g yeast extract, 20 g glucose
Was mixed with 5 L of purified water, put in a jar, and then sterilized at 121 ° C. for 30 minutes. This was attached to a jar fermenter, and 150 mL of a seed mycelium culture of Black Reishi that had been shake-cultured at 25 ° C. for 14 days in the same medium was inoculated. 300r.
p. While stirring at m, aeration of air at 3 L / min, 2
It was cultured at 5 ° C for 14 days. The obtained culture was filtered to obtain 2.0 kg (wet weight) of mycelium. Purified water (2 L) was added to the separated mycelium, and the mixture was extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain 22 g of an extract of a mycelium culture of Black Reishi. .

Example 1 Cream 1 Formulation Compounding amount 1. 0.5 part of hot water extract of Black Reishi (Production Example 1) 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Fragrance 0.1 11.1, 3-butylene glycol 8.5 12. Ethyl paraoxybenzoate 0.05 13. Methyl paraoxybenzoate 0.2 14. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 9 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. Add the water phase to the oil phase to emulsify, cool with stirring and mix at 10
Is added, and the product is further cooled to 30 ° C.

Example 2 Cream 2 In Example 1, 0.5 part of the hot water extract of black ganoderma, 0.25 part of the hot water extract of black ganoderma and the hot water extract of ganoderma (red shiba) were used. (Manufacturing Example 4) Cream 2 was replaced with 0.25 part.

Example 3 Cream 3 In Example 1, cream 3 was prepared by replacing the hot water extract of Black Reishi with an extract of a mycelium culture of Black Reishi (Production Example 7).

Comparative Example 1 Conventional Cream 1 In Example 1, the conventional cream 1 was prepared by replacing the hot water extract of Black Reishi with purified water.

Comparative Example 2 Conventional Cream 2 In Example 1, the hot water extract of Black Reishi was used as Reishi (red shiba).
The conventional cream 2 was prepared by substituting the hot water extract of Example 2 (Production Example 4).

Example 4 Lotion Formulation Amount 1. 50% 1,3-butylene glycol aqueous solution extract of Black Reishi, 10.0 parts (Production Example 3) 2.1,3-butylene glycol 8.0 3. Glycerin 2.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.1 7. Ethanol 5.0 8. Methyl paraoxybenzoate 0.1 9. Polyoxyethylene hydrogenated castor oil (40 EO) 0.1 10. Perfume proper amount 11. [Production method] Components 1 to 6 and 11 and components 7 to 10 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed and filtered to obtain a product.

Example 5 Emulsion 1 Formulation Compounding amount 1. Ethanol extract of Black Reishi (Production Example 2) 0.5 part 2. Squalane 5.0 3. Olive oil 5.0 4. Jojoba oil 5.0 5. Cetanol 1.5 6. Glycerin monostearate 2.0 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Polyoxyethylene sorbitan monooleate 2.0 (20 EO) 9. Perfume 0.1 10. Propylene glycol 1.0 11. Glycerin 2.0 12. Methyl paraoxybenzoate 0.2 13. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 8 are dissolved by heating and mixed, and the temperature is 70 ° C.
Keep it in the oil phase. Ingredients 1 and 10 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the mixture is cooled with stirring, component 9 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Example 6 Emulsion 2 In Example 5, 0.5 part of the ethanol extract of Black Reishi was added to 0.25 part of the hot water extract of Black Reishi (Production Example 1) and the heat of Reishi (Akashiba). Emulsion 2 was prepared by replacing 0.25 part of the water extract (Production Example 4).

Example 7 Gel formulation Formulation amount 1. 50% 1,3-butylene glycol aqueous solution extract of Black Reishi, 0.1 part (Production Example 3) Ethanol 5.0 3. Methyl paraoxybenzoate 0.1 4. Polyoxyethylene hydrogenated castor oil (60 EO) 0.1 5. Fragrance suitable amount 6.1,3-butylene glycol 5.0 7. Glycerin 5.0 8. Xanthan gum 0.1 9. Carboxyvinyl polymer 0.2 10. Potassium hydroxide 0.2 11. [Production method] Components 2 to 5 and components 1 and 6 to 11 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed to obtain a product.

[0032]   Example 8 Pack Prescription amount   1. Hot water extract of Black Reishi (Production Example 1) 0.1 part   2. Reishi (Akashiba) ethanol extract (Production Example 5) 0.1   3. Polyvinyl alcohol 12.0   4. Ethanol 5.0   5.1,3-butylene glycol 8.0   6. Methyl paraoxybenzoate 0.2   7. Polyoxyethylene hydrogenated castor oil (20EO) 0.5   8. Citric acid 0.1   9. Sodium citrate 0.3 10. Fragrance suitable amount 11. Adjust the total volume to 100 with purified water [Production method] Components 1 to 11 are uniformly dissolved to obtain a product.

Example 9 Foundation Formulation Amount 1. 1.0 part of 50% 1,3-butylene glycol aqueous solution extract of Black Reishi (Production Example 3) Stearic acid 2.4 3. Polyoxyethylene sorbitan monostearate 1.0 (20 EO) 4. Polyoxyethylene cetyl ether (20 EO) 2.0 5. Cetanol 1.0 6. Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. Isopropyl myristate 6.5 9. Butyl paraoxybenzoate 0.1 10. Carboxymethyl cellulose sodium 0.1 11. Bentonite 0.5 12. Propylene glycol 4.0 13. Triethanolamine 1.1 14. Methyl paraoxybenzoate 0.2 15. Titanium dioxide 8.0 16. Talc 4.0 17. Red iron oxide 1.0 18. Yellow iron oxide 2.0 19. Perfume appropriate amount 20. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 9 are dissolved by heating and kept at 80 ° C to form an oil phase. The component 10 is swelled well in the component 20, and subsequently, the components 1 and 11 to 14 are added and mixed uniformly.
Components 15 to 18 pulverized and mixed with a pulverizer are added to this, and the mixture is stirred with a homomixer and kept at 75 ° C to form an aqueous phase. The oil phase was added to this aqueous phase while stirring, cooled, and the component 1 was added at 45 ° C.
Add 9 and cool to 30 ° C with stirring to obtain a product.

[0034]     Example 10 Bath agent Prescription amount   1. 1.0 part of hot water extract of Black Reishi (Production Example 1)   2. Sodium hydrogen carbonate 50.0   3. Yellow No. 202 (1) Suitable amount   4. Fragrance suitable amount   5. Adjust the total amount to 100 with anhydrous sodium sulfate [Manufacturing method] Components 1 to 5 are uniformly mixed to obtain a product.

Example 11 Ointment Formulation Amount 1. Ethanol extract of Black Reishi (Production Example 2) 1.0 part 2. Polyoxyethylene cetyl ether (30EO) 2.0 3. Glycerin monostearate 10.0 4. Liquid paraffin 5.0 5. Cetanol 6.0 6. Methyl paraoxybenzoate 0.1 7. Propylene glycol 10.0 8. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 5 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring.

Next, in order to explain the effects of the present invention in detail, experimental examples will be given.

Experimental Example 1 Melanin production inhibitory test using B16 mouse melanoma 3 melanomas in logarithmic growth phase were placed in a 60 mm dish.
E containing 10 ⁴ cells and containing samples of various concentrations
add agles' MEM (containing 10% fetal calf serum),
It was cultured under the conditions of 37 ° C. and 5% CO ₂ . After 5 days of culturing, the cells were detached from the petri dish, the cells were ultrasonically disrupted, 2N aqueous sodium hydroxide solution was added, and the mixture was treated at 60 ° C. for 2 hours and then subjected to OD with a spectrophotometer. D. 475 nm was measured. In addition, the cell lysate after sonication is Lowry
Method (J. Biol. Chem., 193, 265-.
275, 1951) to quantify the protein, and compare the amount of melanin per amount of protein to obtain an index for the melanin production inhibitory action. Ascorbic acid was used as a positive control.

The results of these experiments are shown in Table 1. As a result, the hot water extract of Black Reishi showed a superior melanin production inhibitory action as compared with the hot water extract of Reishi (Akashiba). Furthermore, when the hot water extract of black reishi and the hot water extract of reishi (Akashiba) were used together, a remarkable melanin production inhibitory effect was shown.
In addition, the extracts of other production examples also showed excellent melanin production inhibitory action.

[0039]

[Table 1]

Experimental Example 2 Histamine Release Inhibitory Action The histamine release inhibitory action was measured using mast cells collected from the abdominal cavity of male Spraque-Dawley rats. That is, 1 μg / mL of compound 48
The effect of inhibiting the release of histamine from mast cells by / 80 was determined as the release inhibition rate. Mast cells are Sulli
van et al. (J. Immunology, 114,
1473, 1975) and the histamine quantification was determined by M
The method of Ay et al. (J. Allergy, 46, 12, 19
70). Indomethacin was used as a positive control.

The results of these experiments are shown in Table 2. As a result, the hot water extract of Black Reishi showed a superior histamine release inhibitory effect as compared with the hot water extract of Reishi (Akashiba). Furthermore, the combined use of the hot water extract of black reishi and Reishi (red shiba) showed a remarkable inhibitory effect on histamine release. In addition, the extracts of other production examples also showed excellent melanin production inhibitory action.

[0042]

[Table 2]

Experimental Example 3 Usage Test 1 Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, 30 women (21) suffering from spots and freckles.
-46 years old) was used for a one-month use test. After use, the effect of improving spots and freckles was evaluated by a questionnaire.

The results of these tests are shown in Table 3. As a result, when the creams obtained in Examples 1 to 3 were used,
Compared with the case where the creams obtained in Comparative Examples 1 and 2 were used, the effect of improving skin stains and freckles was shown. That is, the cream containing the hot water extract of Black Reishi and the cream using the hot water extract of Black Reishi and Reishi (Akashiba) in combination exhibited a remarkable effect. During the test period, there was no skin trouble and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0045]

[Table 3]

Experimental Example 4 Usage Test 2 Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, 30 women (19 to 4) suffering from rough skin and dryness.
A one-month use test was conducted for 8 years old. after use,
The effect of improving rough skin and dryness was evaluated by a questionnaire.

The results of these tests are shown in Table 4. As a result, when the creams obtained in Examples 1 to 3 were used,
Compared with the case where the creams obtained in Comparative Examples 1 and 2 were used, the effects of improving rough skin and dryness were exhibited. That is, the cream containing the hot water extract of Black Reishi and the cream using the hot water extract of Black Reishi and Reishi (Akashiba) in combination exhibited a remarkable effect. During the test period, there was no skin trouble and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0048]

[Table 4]

Experimental Example 5 Use Test 3 Using the creams of Examples 1 to 3 and the conventional creams of Comparative Examples 1 and 2, 30 women suffering from wrinkles and tarmi (30 to 5)
A one-month use test was conducted for 8 years old. after use,
The effect of improving wrinkles and tarmi was evaluated by a questionnaire.

The results of these tests are shown in Table 5. As a result, when the creams obtained in Examples 1 to 3 were used,
Compared to the case where the creams obtained in Comparative Examples 1 and 2 were used, the effect of improving wrinkles and tarmi was shown. That is, the cream containing the hot water extract of Black Reishi and the cream using the hot water extract of Black Reishi and Reishi (Akashiba) in combination exhibited a remarkable effect. During the test period, there was no skin trouble and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0051]

[Table 5]

The same usage test was carried out on Examples 4 to 11, and it was safe and showed excellent stains, freckles, and
It showed the effects of improving rough skin, dryness, wrinkles and tarmi.

[0053]

From the above, the extract of Black Ganoderma lucidum of the present invention has a particularly excellent melanin production inhibitory action and histamine release inhibitory action as compared with the extract of Ganoderma lucidum (red turf),
It was also excellent in stability. Furthermore, the combined use of the black reishi and the Reishi (red shiba) extract showed a synergistic effect. Further, the external preparation for skin containing these extracts showed safe and excellent anti-aging, whitening and anti-inflammatory effects.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Tomonori Katata             2-7 Torimicho, Nishi-ku, Nagoya-shi Japan Menard             Cosmetics Research Institute F-term (reference) 4C083 AA082 AA111 AA112 AA122                       AB032 AB232 AB242 AB312                       AB352 AB432 AB442 AC012                       AC022 AC072 AC102 AC122                       AC182 AC242 AC302 AC352                       AC422 AC432 AC442 AC482                       AC542 AC842 AD092 AD112                       AD272 AD352 CC02 CC04                       CC05 CC07 CC12 CC25 DD31                       DD41 EE12 EE13 EE16                 4C088 AA06 AC16 AC17 BA09 BA10                       CA05 CA06 CA08 MA63 NA14                       ZA89 ZB11

Claims (3)

[Claims] 1. A skin external preparation containing an extract of Black Reishi. 2. An external preparation for skin comprising the extract of black reishi and reishi (red shiba). 3. The external preparation for skin according to claim 1, wherein the extract is an extract with a solvent selected from one or more selected from water, lower alcohols and liquid polyhydric alcohols.