KR20190067287A - Anti-aging and anti-allergic cosmetic compositon comprising the extract of the flower, root, callus and seed of Hibiscus syriacus L. as an active ingredient and preparation method of the same - Google Patents

Abstract

The present invention relates to an anti-aging or anti-allergic cosmetic composition comprising a mixture of extracts of flowers, roots, calluses, and seeds of Hibiscus syriacus L. as effective components; and a method of preparing the same. The method comprises a step of extracting the flowers, roots, and seeds of Hibiscus syriacus L. at 60°C using water, ethanol, methanol, butylene glycol, propanediol, or a mixed solvent thereof.

Description

The present invention relates to an anti-aging and anti-allergic cosmetic composition containing an extract of a flower, roots, callus and seed as an active ingredient, and a method for producing the same. and seed of Hibiscus syriacus L. as an active ingredient and preparation method of the same}

The present application relates to an anti-aging and anti-allergic cosmetic composition containing a mixture of extracts of flowers, roots, callus, and seeds of Hibiscus japonica as an active ingredient, and a method for producing the same.

Hibiscus syriacus L. It is a bush that is planted in China and India (India), and is distributed throughout the world. It is distributed in East Asia and Europe. Stems stand upright, branches are split, height is 2-3m. Leaves are alternate phyllotaxis, ovate, broad ovate, shallowly divide into three branches, and have sharp serrations on the edge. The flowers are bloom on both sides of the leaf. The flowers bloom one on each side of the leaf on the upper branch in August-October, and they are reddish purple or white. Calyx is tubular, and is divided into 5 branches. There are 5 petals and the bottom is attached to each other. There are a lot of operations, and the operation table is attached to a bundle. The fruit is capsule, round ovate, ripe when divided into 5 branches. There is a record that it is used for edible, ornamental, and medicinal purposes. The Mugunghwa flower is gorgeous, and five petals are attached to each other at the donation. It is often white and red purple, with dark purplish reddish patterns on the inside (petal top base). It is not a native plant but a planted tree compared to H. hamabo Siebold et Zucc. The leaf is divided into shallow edges and the flower is red or white, not yellow.

Skin is a part of the body that is directly exposed to the external environment. Exposure to excessive ultraviolet rays or pollutants causes skin irritation such as erythema, edema, itching, and inflammatory reaction. These skin troubles not only become cosmetic problems but also cause secondary pigmentation of the substances generated during the inflammatory process and promote the collapse of the skin elastic fibers to increase skin aging. These skin injuries are caused by reactive oxygen species, ROS).

Active oxygen is an unstable oxygen that is completely different from the oxygen we breathe. It destroys cells or breaks the connective tissues of the dermal layer of the skin or causes cross-linking, which can cause wrinkles, skin cancer, cell killing, atopic dermatitis, Causing problems. Superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol remove the active oxygen, but the antioxidant system is inactivated by the age, ultraviolet rays and stress, and the active oxygen is gradually increased It causes skin aging.

Inflammation is a physiological response that protects the body from harmful environmental conditions, such as intrusion of external foreign matter such as bacteria and mechanical damage. This inflammation induces the proliferation of several types of polynuclear leukocytes (PMNs), which defend the body by secreting various kinds of proteolytic enzymes and cytokines. However, the excessive production of proteolytic enzymes from the skin due to hyperinflammation causes damage to the non-connective tissues, and damage to the connective tissues reduces the elasticity of the skin to cause wrinkles as well as cell regeneration and proliferation It has a bad influence.

Allergy is a type of hypersensitivity reaction caused by immune imbalance. It is a reaction that occurs to a specific antigen called allergen, and IgE antibody specific to the antigen is induced to bind to mast cell or basophil . The mast cells or basophils activated by the IgE antibody secrete inflammatory cytokines, prostaglandins, and histamines to cause vasodilation and inflammation, resulting in allergic atopy, asthma, and skin allergy. The incidence of such allergic diseases has been greatly increased, and the importance of mitigating and preventing diseases has been emphasized.

The wrinkles of the skin are said to result from collagen being reduced due to aging and photoaging due to ultraviolet irradiation and skin thinning. Recently, research on skin aging has progressed and the function of collagen has been revealed. Especially, studies on prevention of collagen synthesis or reduction and products using cosmetic composition for skin reinforcement due to this have been put on the market.

Mugungwha is known to contain a large amount of ginseng saponarin, horse divergence, stearic acid, tannic acid, dihydrostroloic acid, mucilage, etc., and in particular, can help the body's physiological activity is reported to contain a large amount of ingredients Researches are being carried out to utilize them as antioxidants or various functional cosmetic ingredients. Under these circumstances, the inventors of the present invention have developed an anti-aging and anti-allergy functional cosmetic composition containing various extracts of flowers, roots, callus, As a result of effortful research, the present application has been completed.

Korean Patent No. 10-1564430 and Registration No. 10-1762750 disclose a prior art related to the present invention. However, the present invention relates to a cosmetic composition containing an extract of Mugunghwa itself, And is heterogeneous. Also, Korean Patent No. 10-1701476 discloses a method for producing an extract of Mugunghwa and a food containing the extract, and the use of a functional cosmetic composition using a mixed extract comprising the same amount ratio of the extract of each part of the Mugungwha plant of the present invention Are neither disclosed nor suggested in any of the above citations.

The object of the present invention is to provide a method for producing a mixed extract of Hibiscus flowers, roots, callus and seeds.

Another object of the present application is to provide a mixed extract of Hibiscus flowers, roots, callus and seeds.

Another object of the present invention is to provide an anti-aging and anti-allergic functional cosmetic composition containing the mixture of extracts of flowers, roots, callus and seeds of Mugunghwa as an active ingredient.

Other objects and advantages of the present application will become more apparent from the following detailed description together with the appended claims and drawings. The contents not described in this specification can be sufficiently recognized and inferred by those skilled in the art or similar fields of the present application, so that the description thereof will be omitted.

Hereinafter, the contents of the present application will be described in detail. On the other hand, the description and the embodiment of the embodiment disclosed in this application can be applied to the explanation and the embodiment of the other aspects with respect to the common matters. Also, all combinations of the various elements disclosed in this application fall within the scope of the present application. In addition, the scope of the present application is not limited by the specific description described below.

In order to accomplish the object of the present application, there is provided a method for preparing a mixed extract of Mugunghwa, which is characterized in that a mixed extract of flowers, roots, callus and seeds of an asparagus plant is obtained as an embodiment. The method comprises the steps of extracting the various parts of the hibiscus, that is, flowers, roots and seeds at a high temperature using water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof to obtain an extract, And extracting the starch extract and mugunghwa callus extract at a blending ratio of the same amount to obtain a mixed extract of flowers, roots, callus and seeds of Mugunghwa. Mixing of the extracts of each part of the plants and the extract of callus horseradish can be carried out using various ratios (W / W) of the above solvents, but preferably the same amount (W / W) can be mixed. The extraction temperature may be in the range of 50 to 90 캜 and may be extracted for 1 to 4 days, preferably at 60 캜 for 1 to 4 days. The mixed solvent may be used in a ratio of 1:10 to 1: 100, but the usable ratio is not limited thereto.

Anti-aging is a concept that includes anti-inflammation, anti-wrinkle, and antioxidant in skin, and can be measured by the amount of reduction of NO production, the amount of collagen production, and the amount of reduction of ROS production, but the biomarkers and measurement methods used are not limited thereto.

The present application provides a method for producing an anti-aging or anti-allergic cosmetic composition containing, as an active ingredient, a mixed extract of a flower, roots, callus, and seeds of Hibiscus as another embodiment. The present cosmetic composition can be formulated into a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, an essence, a shampoo, a surfactant-containing cleansing, a tonic, a scaling and the like. More specifically, it can be manufactured by a formulation such as a soft lotion, a nutritional lotion, a nutritional liquid, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a hair essence,

The present application has an excellent effect of providing an anti-aging or anti-allergic function cosmetic composition comprising a mixed extract of flowers, roots, callus, and seeds of the herbivorous plant as an active ingredient and a method for producing the same.

Fig. 1 is a graph showing the anti-inflammatory activity of Mugungwha seed extract.
FIG. 2 is a graph showing the anti-inflammatory effect of the Hibiscus flower extract.
Fig. 3 is a graph showing the anti-inflammatory activity of the extract of Mugunghwa callus.
Fig. 4 is a graph showing the anti-inflammatory activity of the extract of Aspergillus odorus.
FIG. 5 is a graph showing collagen production proliferative activity of Mugunghwa callus extract.
FIG. 6 is a graph showing collagen production proliferative activity of the Hibiscus flower extract. FIG.
FIG. 7 is a graph showing collagen production proliferative activity of hibiscus root extract. FIG.
8 is a graph showing collagen production proliferative activity of Mugungwha seed extract.
9 is a graph showing the antioxidant efficacy of the Hibiscus flower extract.
FIG. 10 is a graph showing the antioxidant effect of the extract of Mugungwha callus.
11 is a graph showing antioxidant efficacy of Mugungwha seed extract.
Fig. 12 is a graph showing antioxidant efficacy of Hibiscus roots extract.
FIG. 13 is a graph showing the anti-allergic effect of the extract of Aspergillus odorus.
14 is a graph showing the anti-allergic efficacy of the Hibiscus flower extract.
Fig. 15 is a graph showing the anti-allergic efficacy of Mugungwha seed extract.
FIG. 16 is a graph showing the anti-allergic effect of the callus extract of Mugungwha.
17 is a graph showing the antioxidant efficacy of mixed extracts of flowers, roots, callus and seeds of Mugungwha.
18 is a graph showing the anti-allergic efficacy of mixed extracts of flowers, roots, callus and seeds of Mugunghwa.
FIG. 19 is a graph showing collagen production proliferative activity of mixed extracts of flowers, roots, callus and seeds of Mugunghwa.
20 is a graph showing the anti-inflammatory activity of mixed extracts of flowers, roots, callus, and seeds of Mugunghwa.

Hereinafter, the present invention will be described in more detail by way of examples according to the present application. However, the following embodiments of the present application are merely examples of the present application. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and that the scope of the present invention, as set forth in the appended claims, is not limited by these examples. something to do.

Example 1. Preparation of Flowers, Roots, Seeds and Callus Extracts of Mugunghwa Plants and Their Mixed Extracts

The extracts of flowers, roots and seeds of the present invention can be obtained by mixing 1: 10 (w / w) to 1: 1 (w / w) of flowers, roots and seeds of each hibiscus in water, ethanol, methanol, butylene glycol, propanediol, 100 (W / W) at 60 占 폚 for 1 to 4 days.

The callus extract of the present invention was obtained by the following method. First, the seeds of Hibiscus are planted on a medium to induce germination by dark room culture at a temperature of 20 to 27 DEG C (preferably 22 DEG C) and a humidity of 65 to 75% (preferably 55 to 60%), Leafs of leaves of Mugunghwa plants were inoculated into MS basic medium containing indole-3-butyric acid (IBA), sucrose and agar and cultured at 23-27 ℃ to induce callus. Then, the cultured callus of callus was propagated through a subculture, and the callus cultures of the proliferated calli were harvested to remove moisture, followed by drying at a temperature of 45 to 55 ° C. Purified water (or a solvent) was added to the dried callus cultured product to extract hot water (or solvent) to obtain a callus extract.

The extracts of flowers, roots and seeds of callus plants obtained in the above steps and callus extracts were mixed with each other in the same amount (W / W) to obtain a mixed extract thereof.

Experimental Example 1. Measurement of anti-inflammatory activity of flowers, roots, seeds and callus extracts of Hibiscus plants

In order to determine the anti-inflammatory effect of the flowers, roots, seeds and callus extracts of the present invention, RAW 264.7 cells were treated with LPS, which is an inflammation inducing substance, Cells were seeded at 2 × 10 ⁵ cells per well in a 96-well plate and cultured in a 5% CO2 incubator at 37 ° C for 24 hours. After overnight incubation, the cells were replaced with fresh medium. LPS (1 ug / ml) and extracts were added at different concentrations and cultured for 24 hours. After incubation, the supernatant was taken and centrifuged at 13,000 rpm for 3 minutes to collect the supernatant, reacted with Griess reagent 1: 1 and absorbance at 570 nm.

As a result of the measurement, the anti-inflammatory activity of each extract of each of the Mugunghwa plants was measured at 2% LPS concentration (Figs. 1 to 4). Specifically, extracts of Mugungwha, flower, callus and root were 23% The anti-inflammatory activity of inhibition of nitric oxide (NO) production by 19%, 16% and 11% was confirmed. It was confirmed that the extract of Mugungwha extract had the best anti-inflammatory activity among the extracts of each part of the Hibiscus plant according to the present invention.

Experimental Example  2. The flowers, roots, seeds and Callus  Measurement of collagen production proliferation of extract

Dermal fibroblasts (HDFn) were inoculated into 48-well plates at a concentration of 5 × 10 ⁴ cells / well in 0.3 ml of culture medium and cultured at 37 ° C and 5% CO ₂ . After 24 hours, the extracts of Mugungwha were cultured for 24 hours in a fresh medium containing the concentration of each extract. After culturing for 24 hours, the culture broth was collected and the amount of C-terminus of the collagen precursor was measured using an enzymes-linked immunoassay kit (Takara). In addition, a standard curve between collagen concentration of 0 ng and 640 ng was obtained using collagen solution 640 ng / mL, and the amount of collagen in the medium was calculated.

As a result of the calculation, it was confirmed that collagen production proliferation effect of the extracts of the respective plant parts of Mugunghwa plants was observed at the extract concentration of 2% (Figs. 5 to 8). In particular, the extracts of Mugunghwa callus, flower, %, And 5%, respectively. In other words, it was confirmed that the extract of Mugunghwa callus extract showed the best proliferation of collagen.

Experimental Example  3. Flowers, roots, seeds and Callus  Antioxidant efficacy of extract

DCFH-DA (2'7'-dichloro flurescinodiacetate) was used to test the antioxidative activity of flowers, roots, seeds and callus extracts of the present invention. For this purpose, dermal fibroblasts (HDFn) were suspended in DMEM (supplemented with 10% serum and 1% antibiotic) medium, and cultured for 24 hours at 37 ° C in a 5% CO ₂ incubator for 1 × 10 ⁵ cells per well. The extracts of each part of the Hibiscus japonica were added to the dermal fibroblasts obtained in the above step and cultured in a 5% CO ₂ incubator at 37 ° C for 1 hour. Then, 1 mM of H ₂ O ₂ and ₂₀ mM of DCFH-DA After the addition, the excitation was 485 nm and the emission was 535 nm.

spectrofluorophotomer. The antioxidant activity of the test group without the sample was used as a control group and the antioxidant activity was calculated by the following formula (1). All experiments were repeated 3 times.

[Equation 1]

Active oxygen species inhibition rate (%) = control OD-sample treatment group OD / control OD X 100

As a result, the antioxidant activity of the extracts of each of the Mugunghwa extracts was confirmed at the extract concentration of 2% (FIGS. 9 to 12), and the antioxidative activities of the extracts of Mugungwha and callus were 20% and 15% there was. According to the present invention, it was confirmed that the antioxidant effect of the extract of Mugunghwa flower was the best among the extracts of each part of the cows.

Experimental Example 4. Measurement of anti-allergic activity of flowers, roots, seeds and callus extracts of Hibiscus plants

RBL-2H3 cells were suspended in DMEM (Dulbecco's modified Eagle's medium) containing 10% FBS to examine the antiallergic effect of each part of the present invention, flowers, roots, seeds and callus extracts in a 24-well plate To 2 x 10 ⁵ cells per well. After sensitization with 100 ng / mL mouse monoclonal IgE per well, the cells were cultured in a 5% CO ₂ incubator for 24 hours. Cells were rinsed with 500 μl of Tyrode buffer per well and treated with DNP-BSA 100 ng / ml for 1 h at 37 ° C in a 5% CO ₂ incubator for 15 min at 37 ° C. The supernatant was treated with 50 μl of substrate (1 mM p-nitrophenyl-N-acetyl-D-glucosaminide), incubated at 37 ° C in a 5% CO2 incubator for 1 hour, and absorbance was measured at 405 nm using an ELISA reader Respectively.

As a result of the measurement, the antiallergic efficacy of the extracts of the respective hibiscus parts at 2% extract concentration was confirmed (Figs. 13 to 16). Among them, the extract of hibiscus roots showed an antiallergic effect of 27% Excellent.

Experimental Example 5. Anti-inflammation, proliferation of collagen production, antioxidation and anti-allergic activity of mixed extracts of flowers, roots, callus and seeds of Hibiscus plants

Each function was evaluated using the anti-inflammatory, collagen proliferation, antioxidant, and antiallergic activity measurement methods described in Experimental Examples 1 to 4, with mixed extracts of flowers, roots, callus and seeds obtained in Example 1 above. The results of the experiment showed that the mixed extracts of flowers, roots, callus and seeds of Mugungwha showed 25% antioxidant effect (FIG. 17), 20% antiallergic effect (FIG. 18), 16% collagen proliferation effect (FIG. 19) , And 21%, respectively (Fig. 20).

Hereinafter, various cosmetic formulations preferable for using the present invention are disclosed, but it goes without saying that the scope of right of the present invention is not limited to the disclosed formulations.

Production Example 1 toilet water

The lotion containing the mixed extract of flowers, roots, callus and seeds of the present invention (hereinafter referred to as mixed extract) obtained in Example 1 of the present invention was prepared as shown in Table 1 below.

Raw material Raw material Content (% by weight) One The mixed extract of the present invention 10.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

Production Example 2 Lotion

The lotion containing the mixed extract of flowers, roots, callus and seeds of the present invention (hereinafter referred to as mixed extract) obtained in Example 1 of the present invention was prepared as shown in Table 2 below.

Raw material Raw material Content (% by weight) One The mixed extract of the present invention 10.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbidestalate 1.0 7 Chin type mono-stearic acid glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

Production Example 3 cream

The cream containing the mixed extract of flowers, roots, callus and seeds of the present invention obtained in Example 1 of the present invention (hereinafter referred to as mixed extract) was prepared as shown in Table 3 below.

content Raw material Content (% by weight) One The mixed extract of the present invention 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

Production Example 4 Essence manufacturing

The essence containing the mixed extract of flowers, roots, callus and seeds of the present invention (hereinafter, mixed extract) obtained in Example 1 of the present invention was prepared as shown in Table 4 below.

content Raw material Content (% by weight) One The mixed extract of the present invention 10.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application is to be interpreted as being within the scope of the present application, all changes or modifications derived from the meaning and scope of the appended claims and from their equivalents rather than the detailed description.

The present invention relates to an anti-aging or anti-allergic cosmetic composition comprising as an active ingredient a mixed extract of flowers, roots, callus and seed extracts of Hibiscus japonica, and a method of preparing the same. Especially, the present invention relates to cosmetic compositions of various formulations such as creams, lotions, Which is a very useful invention in the natural cosmetics industry.

Claims (6)

Extracting flowers, roots and seeds of each part of the hibiscus flower at 60 ° C using water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof to obtain an extract of each part;
The method comprising the step of mixing the extract of each part obtained in the above step with the extract of Mugunghwa callus to obtain a mixed extract of flowers, roots, callus and seeds of Mugunghwa

2. The method according to claim 1, wherein the mixture of the extract of each part of the cow and the callus extract is mixed with the same ratio (W / W)
3. A mixed extract of Hibiscus ash extracted according to the method of claim 1 or 2.
An anti-aging functional cosmetic composition comprising the extract of claim 3 as an active ingredient
An anti-allergy functional cosmetic composition containing the extract of claim 3 as an active ingredient
The anti-aging or anti-allergy functional cosmetic composition according to claim 4 or 5, wherein the cosmetic composition is any one selected from the group consisting of lotion, lotion, cream and essence

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