KR101896908B1 - Method for preparing camellia infused oil and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a method to manufacture a camellia infused oil through multistage fermentation, capable of being effectively used as an antiaging cosmetic material; and a cosmetic composition containing the same. According to the present invention, the method to manufacture a camellia infused oil through multistage fermentation comprises: a step (A) of mixing a mixture of camellia leaf, flower, and seed meal with purified water, and sterilizing the mixture at 120 to 130°C for 20 to 40 minutes; a step (B) of mixing Aspergillus niger with the mixture to perform fermentation at 28 to 32°C for 60 to 64 hours; a step (C) of sterilizing a fermentation product of the step (B) and mixing Bacillus subtilis with the sterilized fermentation product to perform fermentation at 40 to 50°C for 20 to 30 hours; a step (D) of sterilizing the fermentation product of the step (C) and mixing Lactobacillus sakei therewith to perform fermentation at 35 to 45°C for 20 to 30 hours; and a step (E) of sterilizing the fermentation product of the step (D) and extracting camellia oil from the sterilized fermentation product.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for manufacturing a fused oil which is a camellia through multi-stage fermentation and a cosmetic composition containing the fused oil,

The present invention relates to a method for producing fused oil which is a camellia through multi-stage fermentation and a cosmetic composition containing the same. More specifically, the present invention relates to a cosmetic composition containing fused oil which is a camellia which is excellent in antioxidative activity and excellent in wrinkle- And to use it as a cosmetic material.

Camellia japonica is an evergreen arboreous tree of the dicotyledonous plant. Camellias are often divided into bushes from below. The bark is grayish white, and the compound eye is long oval. Leaves are alternate, oval or long oval. There are wavy sawtooths on the edges of the leaves, shiny and hairless. The flowers are red one at the end of early spring branch. Five to seven petals are combined at the bottom, spread diagonally, have a lot of stamen, and fall off when attached to the petals. Style is divided into three. The fruit is round, round, 3 ~ 4cm in diameter with 3 seeds and contains black-brown seeds. Plants and flowers are ornamental, and seeds are oiled. It is also used as a mordant for lye.

Seeds have been used as cooking oil and cosmetic oil resources. Camellia oil contains 9.1 ~ 11.5% of saturated acids, 85.6 ~ 89.4% of oleic acid, and 1.3 ~ 2.9% of linoleic acid. The stem has been used as a raw material for high-grade charcoal, and in the area without tea trees, young leaf of camellia was used as tea material. In the Oriental medicine book "Honbo Gangmok" . Pharmacological effects of camellia leaves are effective for psoriasis, throat pain and burns. Branches and fruits are responsible for hair dandruff, blood, hemorrhage, hemorrhage, cartilage, menstrual disorders, diuretic, throat pain, intestinal hemorrhage, It is known that there is an effect.

Korean Patent Laid-Open Publication No. 10-2005-0058635 entitled "Camellia extract having anti-inflammatory and antioxidant activity and cosmetic composition containing the same", "camellia extract obtained by supercritical extraction from camellia seeds has excellent anti-inflammatory and antioxidative effects" Korean Patent No. 10-1560526 entitled " Composition for Preventing Skin Contamination Containing Camellia sinensis Extract as an Active Ingredient ", " Combined Extract of Camellia sinensis Extract or Camellia sinensis Extract and Camellia sinensis Extract Extract is a natural or environmental pollutant Has an effect of inhibiting collagenase activity in induced fibroblasts, improving collagen synthesis and DEJ protein expression, thereby preventing skin contamination and thereby improving skin condition.

The present inventors conducted studies to effectively extract components having skin-improving activity contained in camellia as a cosmetic, and multi-stage fermentation of a mixture of camellia leaves, camellia flowers and camellia seeds using specific microorganisms, , It was confirmed that a fused oil which is a camellia which is excellent in antioxidative activity and wrinkle-improving activity can be produced. Thus, the present invention has been completed.

It is an object of the present invention to provide a method for producing a fused oil which is excellent in antioxidation activity and excellent in the effect of improving wrinkles by using multi-stage fermentation.

It is another object of the present invention to provide a cosmetic composition containing fused oil which is a camellia produced by the above method.

(A) mixing purified water with a camellia leaf, a camellia flower and a camellia seed mixture, and sterilizing the mixture at 120 to 130 ° C for 20 to 40 minutes; (B) fermenting the mixture with Aspergillus niger at 28 to 32 ° C for 60 to 64 hours; (C) sterilizing the fermented product of step (B), mixing Bacillus subtilis and fermenting the mixture at 40 to 50 ° C for 20 to 30 hours; (D) sterilizing the fermented product of step (C), mixing Lactobacillus sakei and fermenting the mixture at 35 to 45 ° C for 20 to 30 hours; And (E) sterilizing the fermented product of step (D) and extracting it with a camellia oil.

In the step (A), the mixture is washed and dried, and the camellia leaves, camellia seeds and camellia seeds are pulverized and mixed at a dry weight of 1: 2 to 1: 2: 1 to 2, respectively.

In the step (E), it is preferable that the fermented product of step (D) sterilized is dipped in a camellia oil and extracted at 2-8 ° C for 15-30 days.

According to another aspect of the present invention, there is provided a cosmetic composition comprising 0.1 to 20% by weight of fused oil, which is a camellary produced by the above-described production method, based on the total weight of the cosmetic composition. The cosmetic composition can be used for antioxidation and wrinkle improvement.

The copper foil fused oil produced by the production method according to the present invention is safe for human body and exhibits excellent antioxidative effect and skin wrinkle reducing effect, and thus can be usefully used as an anti-aging cosmetic.

Hereinafter, the present invention will be described in detail.

Infused oil refers to a leached oil that has been dipped in a vegetable oil for a certain period of time and has been subjected to fat-soluble active ingredients. It has an advantage that nutrients contained in vegetable oil used as a solvent together with the active ingredient contained in the extracted raw material can be used together.

Sunflower seed oil, macadamia oil, olive oil and the like are generally used as solvents in the manufacture of the infused oil. Since the activity of the infused oil produced according to the extraction method such as the kind of the extraction source, the type of the oil used as the solvent, the extraction temperature, and the extraction period may differ, it is preferable to determine the activity of the infused oil . ≪ / RTI >

(A) mixing purified water with a camellia leaf, a camellia flower and a camellia seed mixture, and sterilizing the mixture at 120 to 130 ° C for 20 to 40 minutes; (B) fermenting the mixture with Aspergillus niger at 28 to 32 ° C for 60 to 64 hours; (C) sterilizing the fermented product of step (B), mixing Bacillus subtilis and fermenting the mixture at 40 to 50 ° C for 20 to 30 hours; (D) sterilizing the fermented product of step (C), mixing Lactobacillus sakei and fermenting the mixture at 35 to 45 ° C for 20 to 30 hours; And (E) sterilizing the fermented product of step (D) and extracting it with a camellia oil.

First, the mixture of camellia leaves, camellia flower and camellia seed which are the extraction source is sterilized. At this time, it is preferable that the mixture is prepared by mixing washed and dried camellia leaves, camellia seeds and camellia seed pellets in a ratio of 1: 2: 1 to 2: 1 to 2, respectively, by dry weight after pulverization. Compared to extracting camellia leaves, camellia blooms and camellia seeds, when they were mixed in an appropriate ratio, they exhibited superior antioxidative and wrinkle-reducing activities.

Then, the mixture is sterilized. According to one embodiment of the present invention, the mixture is mixed with purified water and sterilized at 120 to 130 ° C for 20 to 40 minutes in a sterilizer. The sterilized mixture is mixed with Aspergillus niger and fermented at 28 to 32 ° C for 60 to 64 hours to conduct primary fermentation.

The fermentation product obtained through the primary fermentation is sterilized in the same manner as in the step (A). Subsequently, Bacillus subtilis is mixed and fermented at 40 to 50 ° C for 20 to 30 hours to conduct secondary fermentation again.

The fermentation product obtained through the secondary fermentation is sterilized by the same method, and Lactobacillus sakei is mixed and further fermented at 35 to 45 ° C for 20 to 30 hours for the third fermentation.

Next, the fermentation product obtained through the third fermentation is sterilized by the same method, and the camellia oil is used as a solvent to produce a camellia fused oil. Preferably, the sterilized fermented product is immersed in a camellia oil and then extracted at 2 to 8 ° C for 15 to 30 days.

Thus, a fermentation product is prepared by applying a three-step fermentation process to a mixture of camellia leaves, camellia flowers and camellia seeds, and then fused with a camellia fuse produced by extracting the camellia oil as a solvent at 2 to 8 ° C for 15 to 30 days The de-oil showed better antioxidative activity and wrinkle-reducing activity than the infused oil prepared without using the three-stage fermentation process of the present invention or using other oils as a solvent.

The fusible oil, which is the camellia produced by the above-mentioned production method, is contained in an amount of 0.1 to 20% by weight based on the total weight of the cosmetic composition. The cosmetic composition may be used for antioxidant and wrinkle-improving purposes.

The formulation of the cosmetic composition in the present invention is not particularly limited, but may be applied to various cosmetic compositions such as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, And may be applied to any one form selected from the group consisting of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow.

      [Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Example 1: Preparation of camellia fermented fused oil

Dried, crushed camellia leaves, camellia flowers and camellia seeds were placed in Petri dishes in an amount of 50 g each, and 300 g of purified water was placed in the flask and sterilized at 121 ° C for 30 minutes using a sterilizer. Then, Aspergillus niger isolated from yeast was treated at 1.0 × 10 ⁸ cfu / mL. It was then fermented in Incubator for 64 hours at 30 ° C. After sterilization at 121 ° C for 30 minutes, Bacillus subtilis was treated at 1.0 × 10 ⁸ cfu / mL and incubated at 45 ° C for 24 hours in an incubator. After sterilization at 121 ° C for 30 minutes, Lactobacillus sakei was treated at 1.0 × 10 ⁸ cfu / mL and incubated at 45 ° C for 24 hours in an incubator. After completion of the fermentation process, the fermentation was completed using a sterilizer, sterilized at 121 ° C for 30 minutes, cooled naturally to room temperature, the fermented product was taken out, immersed in 3L of pressed camellia oil and extracted at low temperature for 15 days at 4 ° C. Thereafter, the mixture was filtered through a 100-mesh filter paper to prepare a camellia fermented fused oil.

Examples 2 to 5: Preparation of camellia fermented fused oil

Dried and pulverized camellia leaves, camellia flower and camellia seed were mixed in a composition ratio shown in the following Table 1 (total of 150 g), and fused oils of camellia fermentation were prepared in the same manner as in Example 1 above.

ingredient Weight ratio Example 2 Example 3 Example 4 Example 5 Camellia leaves One One 2 2 Camellia 2 2 One 2 Camellia seeds One 2 One One

Comparative Example 1: Production of fused oil of camellia fermentation

Dried, crushed camellia leaves, camellia flowers and camellia seeds were placed in Petri dishes in an amount of 50 g each, and 300 g of purified water was placed in the flask and sterilized at 121 ° C for 30 minutes using a sterilizer. Then, Aspergillus niger isolated from yeast was treated at 1.0 × 10 ⁸ cfu / mL. It was then fermented in Incubator for 64 hours at 30 ° C. After sterilization at 121 ° C for 30 minutes, Bacillus subtilis was treated at 1.0 × 10 ⁸ cfu / mL and incubated at 45 ° C for 24 hours in an incubator. After sterilization at 121 ° C for 30 minutes, Lactobacillus sakei was treated at 1.0 × 10 ⁸ cfu / mL and incubated at 45 ° C for 24 hours in an incubator. After completion of the fermentation process, the fermentation was completed using a sterilizer, sterilized at 121 ° C for 30 minutes, naturally cooled to room temperature, taken out of the fermented product, immersed in MCT Oil 3L, and extracted at 4 ° C for 15 days. Thereafter, the mixture was filtered through a 100-mesh filter paper to prepare a camellia fermented fused oil.

Comparative Example 2: Manufacture of fused oil which is camellia

Dried and crushed camellia leaves, camellia seeds and camellia seedlings were dipped in 3 L of camellia oil and extracted at low temperature for 14 days at 4 ° C. Thereafter, the mixture was filtered through a 100-mesh filter paper to prepare a camellia fused oil.

Comparative Example 3: Production of fused oil which is camellia

Dried and crushed camellia leaves, camellia seeds and camellia seedlings were dipped in 3 L of MCT Oil and extracted at low temperature for 14 days at 4 ° C. Thereafter, the mixture was filtered through a 100-mesh filter paper to prepare a camellia fused oil.

Test Example 1: Antioxidant effect test using DPPH method

The antioxidant activity was determined by DPPH radical scavenging method. 56 uL of the sample was dissolved in isooctane, 200 μL of the solution was added to a 25 mL test tube, and 3 mL of 250 uM DPPH isooctane solution was added. The solution was allowed to stand at room temperature for 30 minutes at 517 nm Absorbance was measured. The absorbance of the control group to which no sample was added was measured, and the absorbance was calculated by the following equation. The results are shown in Table 2 below.

Antioxidant activity (%) = (control absorbance - sample absorbance) / (absorbance of control) × 100

division Example Comparative Example One 2 3 4 5 One 2 3 Antioxidant activity (%) 68.8 54.5 61.2 55.4 62.5 48.3 17.5 26.7

As can be seen from the above Table 2, the fused oil, which is a camellia produced by extracting with camellia oil after the multi-stage fermentation of the embodiment of the present invention, is much higher than that of the camellia fused oil produced without using fermentation or using MCT oil solvent Showed excellent antioxidant activity. Among them, the most excellent activity was shown in Example 1.

Test Example 2: MMP-1 inhibitory effect

To confirm the effect of wrinkle improvement on the infused oil prepared in the above Examples and Comparative Examples, normal human fibroblasts were inoculated into each well of a 24-well plate at a density of 1.0 × 10 ⁶ cells in a laboratory condition, ≪ / RTI > for 24 hours. Subsequently, the sample concentration was changed to 500 μg / ml and the control group was replaced with serum-free DMEM medium, and the control group was replaced with the serum-free DMEM medium containing no sample. After culturing, the supernatant of each well was collected and the amount of MMP-1 was measured using the MMP-1 assay kit. TGF-beta (10 ng / mL, Roche, USA) was used as a positive control, and the inhibition rate of MMP-1 production was calculated by the following equation.

Inhibitory rate (%) of MMP-1 production = [1- (amount of MMP-1 in the test group / amount of MMP-1 in the control group)] × 100

division Inhibition rate of MMP-1 production (%) Example 1 75.4 Example 2 57.4 Example 3 65.1 Example 4 61.1 Example 5 64.6 Comparative Example 1 52.1 Comparative Example 2 43.9 Comparative Example 3 45.6 Positive control group
(TGF-beta (10 ng / mL)). 68.5

As can be seen from the above Table 3, the fused oil, which is a camellia produced by extracting with camellia oil after the multi-stage fermentation of the present invention, is free from collagen, which is not fermented or produced using MCT oil solvent, It was found that the production of MMP-1 having the degradation characteristic was greatly suppressed.

Example 6: Preparation of camphor fused oil containing cream

The creams containing the camellia fused oils prepared in the above Examples and Comparative Examples were prepared according to the conventional methods with the compositions and contents shown in Table 4 below.

ingredient Control Example Comparative Example 4 Example 6 The fused oil which is camellia (Comparative Example 1) - 3.0 - The fused oil which is camellia (Example 1) - - 3.0 Wax 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 Sorbitan sesquioleate 0.6 0.6 0.6 Mineral oil 5.0 5.0 5.0 Sorbitan stearate 1.0 1.0 1.0 Stearic acid 1.5 1.5 1.5 Glyceryl stearate / phage-100 stearate 1.0 1.0 1.0 Dipropylene glycol 1.0 1.0 1.0 Carbomer 0.2 0.2 0.2 Triethanolamine 0.2 0.2 0.2 Preservative, fragrance, pigment a very small amount a very small amount a very small amount Purified water To 100 To 100 To 100

Test Example 3: Confirmation of wrinkle improvement effect

In order to confirm the effect of the cosmetic composition containing the camellia fused oil of the present invention prepared by extracting with camellia oil after the multi-stage fermentation using camellia leaves, camellia flowers, and camellia seed pellets, Thirty subjects were tested. In order to confirm the effect of improving the wrinkles of the skin, the creams of the control, comparative, and example were applied to the facial area twice a day for 10 days for 10 weeks. After 4 weeks, the degree of wrinkle improvement was evaluated by image analysis of wrinkles. Immediately after the experiment, the replica was collected from the same area under the eyes, and the density of the wrinkles was measured by two-dimensional analysis of the wrinkles through image analysis . The results of the measurement of the wrinkle density by image analysis are shown in Table 5 as a reduction rate relative to the wrinkle density before use.

division Wrinkle density reduction rate (%) Control Example 10 Example 6 40 Comparative Example 4 31

As shown in the above Table 5, the cream of Example 6 containing the fused oil which is the camellia of the example of the present invention exhibited a wrinkle-reducing effect superior to that of the control or comparative example.

Claims (6)

(A) mixing purified water with a camellia leaf, a camellia flower and a camellia seed mixture, and sterilizing the mixture at 120 to 130 ° C for 20 to 40 minutes;
(B) fermenting the mixture with Aspergillus niger at 28 to 32 ° C for 60 to 64 hours;
(C) sterilizing the fermented product of step (B), mixing Bacillus subtilis and fermenting the mixture at 40 to 50 ° C for 20 to 30 hours;
(D) sterilizing the fermented product of step (C), mixing Lactobacillus sakei and fermenting the mixture at 35 to 45 ° C for 20 to 30 hours; And
(E) sterilizing the fermented product of step (D) and extracting it with a camellia oil. The method according to claim 1, wherein the mixture in step (A) is prepared by mixing 1 to 2: 1 to 2: 1 to 2 of dried camellia leaves, camellia leaves and camellia seeds, Wherein the fusible oil is a camellia. The method according to claim 1, wherein the fermented product of step (D) is sterilized in step (E), and the fermented product is dipped in camellia oil and extracted at 2 to 8 ° C for 15 to 30 days. ≪ / RTI > A cosmetic composition comprising 0.1 to 20% by weight of a fused oil, which is a copper oxide produced by any one of claims 1 to 3, based on the total weight of the composition. 5. The cosmetic composition according to claim 4, wherein the cosmetic composition is for antioxidation. The cosmetic composition according to claim 4, wherein the cosmetic composition is for improving skin wrinkles.