KR20210037338A - Preparation method of infused oil for skin soothing and atopy improvement - Google Patents

Abstract

The present invention relates to an infused oil production method for effectively reducing an extraction period, and a method for producing infused oil for skin soothing and atopic alleviation by using the same. According to the method for producing infused oil of the present invention, by using lecithin which is an emulsifier and through specific sequential steps, an active ingredient of a medicinal plant can be extracted within a short time without destroying the active ingredient. In addition, in the case of infused oil prepared by the method for producing infused oil for skin soothing and atopic alleviation of the present invention, it is possible to reduce a transepidermal moisture loss caused by skin barrier damage. Also, it is possible to effectively reduce the concentration of IgE in blood, and thus the produced infused oil can be useful as a functional cosmetic material or a pharmaceutical material.

Description

Preparation method of infused oil for skin soothing and atopy improvement

The present invention relates to a method of manufacturing infused oil that effectively shortens an extraction period, and a method of manufacturing infused oil having a use of soothing skin or improving atopy by using the same.

The skin of the human body is an indispensable organ for life maintenance that physically and chemically protects the body from the outside world and at the same time performs biochemical functions necessary for metabolism of the whole body. All abnormal findings on human skin are called skin diseases, that is, skin diseases. Since the skin covers the surface of the body, there are many opportunities for direct contact with stimuli or various pathogens from the outside world, and is strongly influenced by the body. In addition, even minor changes in the skin can be seen with the eyes and touched with the hands, and a part of the lesion is collected and examined in histopathological pathology, and it is easy to perform tests such as microbial detection, and it is easy to clarify the diagnosis of individual diseases. , The type reaches a considerable number compared to other organs, and the disease name is also complex and diverse. Relatively common skin diseases include atopic dermatitis, contact dermatitis, seborrheic dermatitis, hives, athlete's foot, jock stiffness, psoriasis, herpes simplex, shingles, dry skin, housewife eczema, and acne.

Atopic dermatitis (AD), one of the skin diseases, is caused by multifactorial factors such as immunological, genetic, pharmacological and physiological, and environmental factors.In particular, the etiology and pathogenesis are infection, stress, season and climate. Changes, irritation and allergens are the main causes. Atopic dermatitis is a chronic inflammatory skin disease that is widely caused from infants to adults.

Although the etiology of atopic dermatitis is not yet clear, studies to date have shown that T lymphocytes are burned due to high sensitization reactions caused by an increase in immunoglobulin E (IgE) antibodies or by a decrease in cell-mediated immune function. The lack of functionality due to crack differentiation and blockade of adrenaline receptors present in the skin are presumed to be the cause of the invention.

For the treatment and management of atopic dermatitis, in most dermatological treatments, it is common to prescribe a steroid hormone, that is, a topical corticosteroid, simultaneously to improve the inflammatory reaction together with a moisturizer that maintains moisture on the skin surface. However, when the topical corticosteroid is used for a long period of time, there is a problem in that it causes various side effects of the skin, such as skin atrophy, vasodilation, pigment loss and the occurrence of dilatation striae.

Accordingly, many attempts have been made to find a therapeutic agent with high therapeutic effect and low side effects from natural products with proven safety. Natural plants and herbal medicines are mainly known as anti-atopy-related substances.

Meanwhile, infused oil refers to an oil obtained by extraction using oil as an extraction solvent when extracting the oil component of a plant. These infused oils have a synergistic effect with the functional ingredients of oil used as a solvent to extract the oil components of plants and the active ingredients of oil extracted from plants, resulting in an increase in the content of active ingredients and improved functionality. It has an advantage that can be applied to various products.

However, the conventional manufacturing process of infused oil has a disadvantage that it takes a long extraction period to dissolve the active ingredient in the oil, and to shorten the extraction period, heat may be applied. There is a problem of destroying the activity of.

Therefore, the present inventors studied to find a process that can extract the active ingredient in a short time without destroying the active ingredient of medicinal plants, and as a result, manufacturing infused oil through a specific sequential step using lecithin, an emulsifier. In this case, the present invention was completed by confirming that the active ingredient can be sufficiently dissolved in the oil even in a very short extraction period for 1 to 6 days without the process of applying heat to the oil.

Korean Patent Registration No. 10-1896908 Korean Patent Registration No. 10-1943346

Accordingly, an object of the present invention is to provide a method for producing infused oil that can effectively shorten the extraction period.

Another object of the present invention is to provide a method for preparing infused oil having excellent skin soothing or atopy improving activity by using the above manufacturing method.

In order to achieve the object of the present invention as described above,

The present invention comprises the steps of: a) preparing a first mixture by mixing lecithin and 1,2-hexanediol; b) preparing a second mixture by adding and mixing an aqueous component to the first mixture; c) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture; d) preparing a fourth mixture while stirring after adding oil to the third mixture; And e) aging the quaternary mixture for 1 to 6 days.

In one embodiment of the present invention, the lecithin may be mixed in an amount of 5 to 10% by weight.

In one embodiment of the present invention, the aqueous (aqueous) component may be added in an amount of 5 to 15% by weight.

Of the present invention In one embodiment, the medicinal plant extract may be added in an amount of 1 to 5% by weight.

In one embodiment of the present invention, the oil may be added in an amount of 70 to 80% by weight.

In one embodiment of the present invention, the method of manufacturing the infused oil can shorten the extraction period.

In addition, the present invention comprises the steps of: i) preparing a first mixture by mixing lecithin and 1,2-hexanediol; Ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and then mixing them; Iii) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture; Iv) preparing a fourth mixture while stirring after adding oil to the third mixture; And v) aging the quaternary mixture for 1 to 6 days, providing a method of preparing infused oil for soothing skin or improving atopy.

In one embodiment of the present invention, the medicinal plant extract may be made of yakmomile extract, malt extract, dango elm bark extract, precliper extract, changjigyeongi leaf extract, dapssari extract, purslane extract, and propolis.

In one embodiment of the present invention, the oil may be made of camellia oil, jojoba oil, and olive oil.

In addition, the present invention provides an infused oil for skin soothing or atopy improvement prepared by the above method.

In the method for preparing infused oil of the present invention, the active ingredient can be extracted in a short time without destroying the active ingredient of the medicinal plant by going through a specific sequential step using lecithin, an emulsifier. In addition, in the case of the infused oil produced by the method of preparing the infused oil for skin soothing or atopy improvement of the present invention, transepidermal moisture loss due to damage to the skin barrier can be reduced, and the concentration of IgE in the blood can be effectively reduced. Bar, it can be usefully used as a functional cosmetic material or a pharmaceutical material.

Figure 1 schematically shows the manufacturing process of the infused oil for skin soothing or atopy improvement of the present invention.
Figure 2 is a graph showing the measurement of transepidermal moisture loss according to treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> in an animal model of atopic dermatitis (Normal : Normal mouse, Vehicle: negative control, BM: positive control).
3 is a graph showing the measurement of skin thickness according to treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> in an animal model of atopic dermatitis (Normal: normal Mouse, Vehicle: negative control, BM: positive control).
4 is a graph showing the measurement of blood IgE concentration according to treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> in an animal model of atopic dermatitis (Normal: Normal mouse, Vehicle: negative control, BM: positive control).

The present invention comprises the steps of: a) preparing a first mixture by mixing lecithin and 1,2-hexanediol; b) preparing a second mixture by adding and mixing an aqueous component to the first mixture; c) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture; d) preparing a fourth mixture while stirring after adding oil to the third mixture; And e) aging the quaternary mixture for 1 to 6 days.

The term'infused oil' referred to in this specification is O'Neill which is used by immersing herbs or plants' flowers or roots in vegetable oil and applying heat, or brewing in sunlight for several weeks and taking the last remnants. In other words, it means brewed oil. Since these infused oils have a very high content of vitamins extracted from natural plants, they provide rich moisture and nutrients to the skin to make skin smooth, and natural pigments come out without additional coloring. As for infused oil, Sain John's Wort (Sung Yohan Full) and Calendula are known as representative.

The conventional manufacturing process of infused oil has a disadvantage that it takes a long extraction period to dissolve the active ingredient in the oil, and it may undergo a process of applying heat to shorten the extraction period, but such a process does not activate the active ingredient. There has been a problem of destroying.

In the present invention, it has been devised to solve the conventional problem as described above, and has a characteristic of extracting the active ingredient in a short time without destroying the active ingredient of the medicinal plant by going through the steps a) to e) sequentially. .

Step a) of the present invention is a step of preparing a first mixture by mixing an emulsifier and a preservative, in detail, a water in oil (W/O type) emulsifier capable of forming a water-oil (WO) interface. In this step, a first mixture is prepared by mixing phosphorus lecithin and 1,2-hexanediol as a preservative.

In one embodiment of the present invention, the lecithin may be mixed in 5 to 10% by weight, preferably 6 to 8% by weight.

Step b) of the present invention is a step of adding and mixing an aqueous component to the lecithin emulsifier composition, and in detail, adding an aqueous component to the first mixture prepared through step a) and mixing This is the step of preparing a mixture.

The term "aqueous component" referred to herein refers to a component having the property of water.

In one embodiment of the present invention, the aqueous (aqueous) component may be added in an amount of 5 to 15% by weight.

Step c) of the present invention is a step of mixing by adding a medicinal plant extract, in detail, preparing a tertiary mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture prepared through step b). to be.

The term "medicinal plant" referred to herein refers to a herb or plant capable of imparting a pharmacological action in infused oil.

In one embodiment of the present invention, the medicinal plant extract may be added in an amount of 1 to 5% by weight.

Step d) of the present invention is a step of adding and mixing oil, in detail, adding oil to the tertiary mixture prepared through step c) and then preparing a fourth mixture while stirring.

The term “oil” referred to in the present specification refers to an oil that is generally vegetable, has a long storage period, and is stable as a solvent for brewing an active ingredient. For example, olive oil, macadamia oil, sunflower seed oil, chapula oil, sweet almond oil, peanut oil, safflower seed oil, camellia oil, jojoba oil, and the like may be exemplified, but are not particularly limited thereto.

In one embodiment of the present invention, the oil may be added in an amount of 70 to 80% by weight.

The method for producing infused oil including steps a) to e) of the present invention can effectively shorten the extraction period compared to the prior art.

In addition, the present invention comprises the steps of: i) preparing a first mixture by mixing lecithin and 1,2-hexanediol; Ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and then mixing them; Iii) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture; Iv) preparing a fourth mixture while stirring after adding oil to the third mixture; And v) aging the quaternary mixture for 1 to 6 days (storing at room temperature), providing a method for preparing infused oil for soothing skin or improving atopy.

In one embodiment of the present invention, the medicinal plant extract may be made of yakmo wheat extract, malt extract, dango elm bark extract, precliper extract, changjigyeongi leaf extract, dapssari extract, purslane extract, and propolis.

In another embodiment of the present invention, the oil may be made of camellia oil, jojoba oil and olive oil.

In addition, the present invention provides an infused oil for skin soothing or atopy improvement prepared by the above method.

In addition, the present invention provides a functional cosmetic composition comprising the infused oil for skin soothing or atopy improvement as an active ingredient.

The infused oil of the present invention may be included in an amount of 0.00001 to 20.0% by weight based on the total weight of the cosmetic composition.

If the content of the infused oil is less than 0.00001% by weight, it is not preferable because the intended skin soothing or atopy improvement effect cannot be sufficiently achieved.If the content exceeds 20% by weight, there is a problem in the formulation stability of the cosmetic. Can occur.

The cosmetic composition of the present invention includes moisturizers, thickeners, surfactants, emulsifiers, preservatives, antioxidants, alcohols, fragrances, pH adjusters, natural extracts, etc., as well as skin within the scope of not impairing the effects of the present invention other than the infused oil. Chemical ingredients for doubling the soothing or atopy improvement effect can be blended in an appropriate amount.

On the other hand, the cosmetic composition prepared by containing the infused oil may be prepared in various formulations such as lotions, essences, skins, lotions, creams, and hemp, specifically hair conditioner, hair tonic, It may be prepared in the form of hair cream, hair lotion, hair oil, hair spray, shampoo or conditioner, and may be prepared using a conventional cosmetic preparation method.

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin soothing or atopic dermatitis comprising the infused oil as an active ingredient.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

< Example 1>

Of the present invention Skin soothing And for atopy improvement Infused Oil manufacturing

<1-1> Material preparation

The source of purchase of the material used in the manufacture of the infused oil of the present invention is as follows.

Ingredients Used to Make Infused Oil and Where to Buy It Ingredients Where to buy lecithin Amikos Co., Ltd. 1, 2-hexanethiol Amikos Co., Ltd. Chamomile flowers Amikos Co., Ltd. Aloe Vera Leaf Juice Amikos Co., Ltd. Yakushi extract Amikos Co., Ltd. Malt extract Amikos Co., Ltd. Tang Elm Bark Extract Amikos Co., Ltd. Preclipair extract Amikos Co., Ltd. Changjilgyeongi Leaf Extract Amikos Co., Ltd. Dapsari extract Amikos Co., Ltd. Purslane extract Amikos Co., Ltd. Propolis extract Amikos Co., Ltd. Camellia oil Amikos Co., Ltd. Jojoba Oil Amikos Co., Ltd. Olive oil Amikos Co., Ltd.

<1-2> of the present invention Infused Oil manufacturing

In order to prepare the infused oil of the present invention, first, lecithin (7% by weight) + 1,2-hexanediol (2.7% by weight) were mixed. Thereafter, chamomile flower water (6.9% by weight) and aloe vera leaf juice (6.1% by weight) were added to the mixture, followed by mixing, and then, extract powders of eight medicinal plants (2.4% by weight = Yakushi extract, malt extract, sugar elm bark) Extract, Precliper extract, Changjilgyeongi leaf extract, dappsari extract, purslane extract, and propolis extract) were added and the plant extract powder was dissolved while stirring at room temperature at 5000 rpm. Thereafter, camellia oil (37% by weight), jojoba oil (10% by weight), and olive oil (27.9% by weight) were added and stirred again at room temperature at 5000 rpm. Finally, the raw material was sealed and aged for one week to prepare the infused oil of the present invention. For reference, the 8 kinds of medicinal plant extract powders were mixed and used in the same amount of each.

< Comparative example 1>

Through conventional methods Infused Oil manufacturing

Camellia oil (40% by weight), jojoba oil (15% by weight), and olive oil (30% by weight) 10 kinds of medicinal plant powders (5% by weight = Yakushi extract, malt extract, sugar elm bark extract, Precliper extract , Changjigyeongi leaf extract, dappsari extract, purslane extract and propolis extract) were added and mixed to prepare infused oil while stirring at a temperature of 32 to 38°C for 3 to 5 weeks. For reference, the 10 kinds of medicinal plant extract powders were mixed and used in the same amount of each.

< Experimental example 1>

Of the present invention Infused Oil's inhibitory effect on atopic dermatitis

<1-1> Atopic dermatitis animal model production and Infused Oil treatment

In order to investigate the atopic dermatitis inhibitory effect of the infused oil of the present invention, oxazolone, a hapten-forming substance that artificially induces dermatitis, was used in BALB/c mice (Orient Bio Inc., Seongnam, Republic of Korea). ) Was applied to the back of atopic dermatitis-like mouse model, and then the effects of the treatment of the infused oil of the present invention on the symptoms of atopic dermatitis were examined.

First, after hair removal on the back of the mouse cleanly, atopic dermatitis was repeated by applying a 10, 0.3% concentration of oxazolone solution (a solution of 10% and 0.3% of oxazolone dissolved in ethanol solvent) to the back of the mouse for 16 days. Was induced, and 50 μl each of the infused oil of the present invention prepared through <Example 1> or the infused oil prepared through <Comparative Example 1> twice daily from the 7th to the 16th of application Treated on the skin. On the other hand, as a positive control group, betamethasone, which has the largest anti-inflammatory action among steroids, was used at a concentration of 0.01%. When 24 hours elapsed after the last treatment, the mice were sacrificed and used in the following experiment.

<1-2> due to damage to the skin barrier Transepidermal moisture loss Measure

In this experiment, TEWL was measured using a vapometer (Delfin®) and measured in units of the amount of water lost per unit area and per hour (g/m ² h).

As a result, as shown in FIG. 2, it was confirmed that the degree of transepidermal moisture loss was reduced when the infused oil of the present invention was treated (applied) to the mouse causing atopic dermatitis. The above results indicate that the infused oil treatment (application) of the present invention can suppress the incidence of atopic dermatitis symptoms by reducing damage to the skin barrier and effectively reducing skin moisture loss. In particular, it was confirmed that the infused oil of <Example 1> of the present invention can more effectively reduce skin moisture loss compared to the infused oil of <Comparative Example 1>.

<1-3> Measurement of skin thickness

In this experiment, thickness (mm) was measured using a caliper as a folding method in order to evaluate the degree of hyperproliferation of the epidermis as an indicator of the inflammatory response.

As a result, as shown in FIG. 3, it was confirmed that the thickness of the epidermal layer of the mouse was decreased when the mouse inducing atopic dermatitis was treated (applied) with the infused oil of the present invention. In particular, the effect of reducing the thickness of the mouse epidermal layer was not significant when treated with the infused oil of <Comparative Example 1>, but the thickness of the epidermal layer of the mouse was significantly reduced when the infused oil of <Example 1> of the present invention was treated. Appeared to be.

<1-4> in blood IgE Concentration measurement

On the end of the experiment, blood was collected from the orbital vein of the mouse and then centrifuged for 3 minutes at 5,000 rpm and 4°C to separate the serum. It was stored in a -70°C freezer until it was used in the experiment, then melted and used in the experiment. The concentration of serum IgE in hairless mice was measured using an ELISA kit for mice (SHIBAYAGI Co., Ltd, Japan), respectively. IgE was quantified according to the method of the ELISA kit for IgE mice (SHIBAYAGI Co., Ltd, Japan). All reagents were adjusted to room temperature and used immediately after preparation.

In detail, after washing the plate three times with the washing buffer, 50 μl of the IgE standard solution or sample solution (sample 5 μl + buffer 45 μl) was dispensed into each well, gently mixed with a microplate shaker, and then at room temperature for two hours. After incubation and reaction at (20-25°C), the plate was washed 3 times with a buffer solution. Dispense 50 μl of biotin-conjugated anti-IgE antibody into each well, mix lightly with a microplate shaker, incubate for two hours at room temperature, react, and wash the plate three times with a washing buffer. Then, 50 μl of HRP-avidin solution was added to each well, mixed lightly with a microplate shaker, and incubated for an hour at room temperature to react, 50 μl of a chromagenic substrate solution was added, and a microplate shaker (microplate shaker). shaker), and then incubated at room temperature for 20 minutes to react, 50 μl of a reaction stopper was added to each well, mixed well to stop discoloration, and the absorbance was measured at 450 nm within 30 minutes.

As a result, as shown in FIG. 4, when the mouse inducing atopic dermatitis was treated (applied) with the infused oil of <Example 1> of the present invention, the negative control group (vehicle) and the infused oil of <Comparative Example 1> Compared to the oil-treated group, it was confirmed that the concentration of IgE in the blood was significantly reduced. In particular, it was found that the concentration of IgE in blood was further reduced in the experimental group treated with the infused oil of <Example 1> of the present invention compared to the positive control group treated with betamethasone.

< Experimental example 2>

Of the present invention Infused Oil skin irritation test

Using a band-aid for irritation test, absorb 1 ml of the infused oil of the present invention into the gauze part of the band-aid, and then attach it on the upper arm of a person. After attaching, the bandage was removed after 48 hours, and the skin was observed 1 hour later. The evaluation method was evaluated according to the evaluation table below.

The determination was conducted on 50 subjects (total of 100 subjects) for each example, and the results are shown in the following table.

Skin irritation evaluation table rank state 0 There is no reaction to the skin at all One Partial skin erythema occurs 2 Overall skin erythema occurs 3 Skin erythema occurs and the skin is swollen 4 Redness of the skin occurs and the skin is severely cracked.

Skin irritation of the infused oil of the present invention Evaluation item Comparative Example 1 Example 1 Skin irritation 0.1 0.1

As shown in Table 3, in the case of the infused oil of <Comparative Example 1> and <Example 1>, it was found that there was little skin irritation, and it was confirmed that it was stable to the skin when used.

< Experimental example 3>

Of the present invention Infused Of oil Moisturizing power , Wound healing effect, itching relief effect, skin strengthening and atopic skin increase relief effect experiment

About 50 patients with atopic dermatitis divided by 25 patients into 2 groups for the moisturizing power, wound healing effect, itching symptom relief effect, skin strengthening, and atopic skin symptom relief of the infused oil of the present invention. The effect was evaluated by using it over the area showing symptoms of atopic dermatitis.

At this time, the evaluation was evaluated according to the criteria of very excellent 10 points, excellent 8 points, average 6 points, bad 4 points, and very bad 2 points, and the results are shown in Table 4.

Evaluation of the moisturizing power, wound healing effect, itchiness relief effect, skin strengthening and atopic skin increase relief effect of the infused oil of the present invention Evaluation item Comparative Example 1 Example 1 Moisturizing power 8.6 9.0 Wound healing 7.0 9.2 Relieve itching 5.0 9.2 Skin strengthening 7.0 8.8 Relieve atopic skin symptoms 5.0 9.4

As shown in Table 4, in the case of the infused oil of <Example 1> of the present invention, compared to <Comparative Example 1>, the moisturizing power, wound healing effect, itchiness relief effect, skin strengthening and atopic skin symptom relief effect It could be confirmed that it was excellent.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the above description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (10)

a) preparing a first mixture by mixing lecithin and 1,2-hexanediol;
b) preparing a second mixture by adding and mixing an aqueous component to the first mixture;
c) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture;
d) preparing a fourth mixture while stirring after adding oil to the third mixture; And
e) A method for producing an infused oil comprising the step of aging the fourth mixture for 1 to 6 days. The method of claim 1,
The method, characterized in that the lecithin is mixed in an amount of 5 to 10% by weight. The method of claim 1,
The method characterized in that the aqueous (aqueous) component is added in an amount of 5 to 15% by weight. The method of claim 3,
The method characterized in that the medicinal plant extract is added in an amount of 1 to 5% by weight. The method of claim 1,
The method, characterized in that the oil is added in an amount of 70 to 80% by weight. The method according to any one of claims 1 to 5,
The method of manufacturing the infused oil, characterized in that the extraction period is shortened. I) preparing a first mixture by mixing lecithin and 1,2-hexanediol;
Ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and then mixing them;
Iii) preparing a third mixture while stirring at room temperature after adding the medicinal plant extract to the second mixture;
Iv) preparing a fourth mixture while stirring after adding oil to the third mixture; And
V) A method for producing infused oil for skin soothing or atopy improvement, comprising the step of aging the fourth mixture for 1 to 6 days. The method of claim 7,
The medicinal plant extract is a method characterized in that consisting of yakmo wheat extract, malt extract, dango elm bark extract, precliper extract, changjikyeongi leaf extract, dappa extract, purslane extract, and propolis. The method of claim 7,
The method, characterized in that the oil consists of camellia oil, jojoba oil and olive oil. Infused oil for soothing skin or improving atopy prepared by the method of any one of claims 7 to 9.

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