KR20100027359A - A composition for improving atopic dermatitis containing extracts from guaiacum - Google Patents

Abstract

PURPOSE: A composition containing Guaiacum extract for relieving atopic dermatitis is provided to relieve itch of atopic dermatitis with safety to human body and prevent resistant bacteria. CONSTITUTION: A composition for relieving atopic dermatitis contains essential oil extract from leaves, stem, or wood of Guaiacum as an active ingredient. The extract is prepared through supercritical fluid extraction, stream extraction and solvent extraction. The composition contains 0.1-30 weight% of the extract. The composition is used in the form of skin lotion, nutrition lotion, nutrition cream, massage cream, cleansing foam, cleansing water, and pack.

Description

Composition for Improving Atopic Dermatitis Containing Extracts from Guaiacum

The present invention relates to a composition for improving atopic skin containing essential oil components of lychee tree, and more particularly, to provide atopic skin improvement effect by containing protein and / or recombinant human epidermal growth factor of sap coming from fluent tree. It relates to a composition.

Atopic skin is named for innate hypersensitivity to certain types of substances that are unique to humans and means allergic predispositions that appear either household or genetically. However, recent studies have shown that environmental pollution not only makes the genetic potential predisposition of atopic skin more powerful, but also causes atopic skin in people without genetic predisposition. Atopic skin is an overreaction of the immune system to changes in the environment (food, climate, and certain substance changes). Atopic skin symptoms, even in people without genetic predisposition, because environmental pollution destroys the human immune system. This is described as frequent.

Symptoms of atopic dermatitis are high fever, asthma, and atopic dermatitis, which are often related to family history, and have a high positive rate of intradermal reactions to various allergens (eg, egg white, pollen, dust, etc.) Allergic support) is present and may respond abnormally to various stresses (temperature, humidity, mental strain, infection, etc.). Atopic dermatitis, also called atopic dermatitis, is associated with allergic diseases such as chronic asthma and hay fever.

On the other hand, about 20% (9,535,246) of Koreans suffer from atopic dermatitis. Among them, 25% (2,383,811) in infants (under 2 years), 70% (6,674,672) in childhood (2-10 years), Adolescents and adults (11 years of age or older) develop 5% (476,762).

Patients are treated with steroid ointments and oral medications (including injections) at hospitals, clinics and pharmacies when atopic dermatitis develops. Side effects such as erythema have been reported. Side effects such as endocrine system, secondary adrenal insufficiency, diabetes, digestive system side effects, peptic ulcer, gastritis, psychiatric nervous system side effects, depression, headache, musculoskeletal side effects, osteoporosis, protein metabolism Side effects include night dogs, nitrogen imbalance, and electrolyte side effects, which are known to cause increased blood pressure, increased intraocular pressure, glaucoma, and other effects of skin infections, steroid acne, steroid dermatitis, and pituitary adrenal glands. It has serious side effects such as suppression of system function. It is.

In particular, 1,787,858 (75% of 2,383,811 infants) occurred before the age of 1, so the side effects of using steroids are more serious than adults.

In addition, atopic dermatitis has been shown to be the main cause of IgE production. As a therapeutic agent, interferon γ and interferon α (Hanifin JM et al., J.AM Acad, dermatol. 1993; 28, 187-97) Although it is used, there is a tendency to relapse within a short time after the administration of the drug, and due to side effects, it is not suitable for long-term administration, the situation is required to develop a rapid drug.

Recently, a bacterium called Staphylococcus aureus, known as Staphylococcus aureus, is known to form colonies, increase their amount, and play an important role in the development and exacerbation of atopic dermatitis.

In other words, the severity of atopic dermatitis correlated with the density of Staphylococcus aureus and the number of bacteria decreased significantly according to clinical improvement. The Staphylococcus aureus is detected in less than 5% of the normal human skin, but in more than 90% of the lesions of atopic dermatitis, it secretes various exotoxins and these exotoxins act as super antigens. It aggravates atopic dermatitis by stimulating skin lesions and inflammatory cells in the blood. In particular, exocytosis secreted by Staphylococcus aureus acts as a superantigen that stimulates T cells 1000 times more powerfully than conventional antigens, exacerbating and sustaining inflammation.

Therefore, based on these findings, there is a report that the combination of antibiotics targeting Staphylococcus aureus (TARGET) showed a better effect than the use of conventional steroids alone for the treatment of atopic dermatitis. However, the emergence of resistant bacteria is also emerging as a side effect of the use of antibiotics.

In fact, in the treatment of Staphylococcus aureus infection, penicillin has been widely used since the 1950s, but since 1964, more powerful antibiotics have been developed due to the abuse of antibiotics. did.

In addition, Korean Patent Application No. 10-2002-0060286 applying antimicrobial components derived from natural products and preventing the emergence of resistant bacteria due to overuse of these antibiotics has been proposed for atopic dermatitis alleviation and prevention cosmetic composition containing cinnamon extract. No. 10-2003-0038851 contains extracts obtained by mixing soybean fermented by Baek, Jaso, Echinacea and Bacillus subtilis in a weight ratio of 2 to 30: 1 to 25: 1 to 25: 6 to 35. Cosmetics having improved atopic healing properties have been disclosed, but there is still a need for a composition derived from natural products which is safe for the human body and is more effective in treating and preventing atopic skin.

There is a therapeutic and preventive effect on the atopic skin as described above, according to the needs of a safe composition for the human body to use the fluency wood to develop the composition.

Yuchang-mok (木, guaiacum) is an evergreen tree of the dicotyledonous rats, the genus Acacia, and its scientific name, Guaiacum officinale, is distributed in the West Indies, southern Mexico, and Central and South America. It is a small tree about 15m high and grows very slowly. The leaves are opposite each other. Even leaflets have 4 to 6 small leaves.

The leaves are broad egg-shaped, leathery, and about 3cm long. Sapwood is yellow, heartwood is greenish brown, dense, hard, one of the hardest trees, and strong. Wood has acidity and aroma.

If you use fluent wood in Korean, it is a tree that heals wounds. It is a little unfamiliar because of its growth in South America, which is the opposite of Korea, but is widely known in the United States and Europe. It is a small tree growing about 15m in height that grows in the Great Plains (Greenchao) region of South America. In the United States, it is called lignum bait (life tree) or green heart (green heart), and in Spanish it is called palosanto (sacred tree).

Ebony is often known as the world's largest tree, but the largest tree is fluent. The proportion of ebony is about 1.1, and that of changchang is about 1.37. It is not only hard but also durable. Thus, until the early 20th century, it was used as a screw shaft or bowling ball for ships.

Ryuchang is used for the construction of mechanical parts that require lubricating oil because it contains resin, and it is also used for furniture and handicrafts. The flowers are light blue and run in inflorescences at the end of branches. Fruits are lichen and ripe when they become yellow or orange. The color of the tree is yellow but turns greenish brown when exposed to the sun. Wood has acidity and aroma.

The sap from the spearwood contains gutta-percha-like resin and saponin, which acts as an antioxidant, and has a beneficial effect on the human body such as sterilization, disinfection, anti-inflammatory, deodorization, detoxification, cell growth, anti-virus, antioxidant, and diuresis. Because of this, it has been used for the treatment of chronic skin diseases such as rheumatoid arthritis, seborrheic dermatitis and atopy, cancer laryngitis and even syphilis.

Resin is used as an antioxidant for oils and fats and is used as a medicine along with timber and bark. G. sanctum, native to the West Indies, is similar to lychee tree and is used for the same purpose.

The value of fluent wood is in incense rather than hardness and specific gravity of wood. If you hold it in your hand, the essence oil will bleed out constantly enough to make the moisture feel subtle. It is known that this balm heals many diseases.

As described above, the present invention was completed according to the necessity of a natural product composition having improved atopy healing effect, safe for human body, and excellent treatment and prevention effect for atopic skin by using the characteristics of the fluent tree as described above.

The present invention relates to a composition for improving atopic skin containing essential oil components, which are safe for the human body, alleviate the itch of atopic skin diseases, and improve or prevent atopic skin, and prevent the appearance of resistant bacteria. It is to provide a cosmetic composition for improving atopic skin.

An object of the present invention can provide a composition for improving atopic dermatitis comprising an essential oil component extract extracted from fluent wood as an active ingredient.

At this time, according to an additional feature of the present invention, the composition is preferred to improve atopic skin through antibacterial, antifungal, free radical scavenging, anti-inflammatory and moisturizing action.

In addition, according to an additional feature of the present invention, it is preferable that the content of the lychee tree extract in the composition is 0.1 to 30% by weight.

In addition, according to an additional feature of the present invention, it is preferable that the formulation of the cosmetic composition consists of skin lotion (cosmetic water), nutrition lotion, nutrition cream, massage cream, face wash foam, face wash water, pack or baby powder.

According to the present invention, the cosmetic composition for improving atopic skin containing essential oil of changwoo wood is safe for the human body, alleviates the itch of atopic skin diseases, and improves or prevents atopic skin disease, and prevents the appearance of resistant bacteria. The derived composition can be provided.

In order to achieve the above object, the present invention provides a composition for improving atopic dermatitis comprising essential oil extract extracted from the leaves, stems or wood flour of lychee tree.

Hereinafter, the present invention will be described in detail.

The present inventors confirmed that essential oil extracts extracted from the leaves, stems, or wood meals of the lychee tree have a very excellent therapeutic and preventive effect on atopic skin and are safe for the human body and have no side effects such as the appearance of resistant bacteria. To complete.

In the present invention, the method for obtaining the essential oil component extract of lychee tree may be applied to conventional methods of extracting the active ingredient from the plant. One example is a supercritical fluid extraction method, steam extraction method or solvent extraction method.

Preferably, since the extract is obtained at a relatively low temperature, it is possible to prevent decomposition and deterioration due to heat, and to separate the components and the like to have excellent selectivity, and to use a supercritical fluid extraction method for obtaining a high purity product. In addition, the stem of the fluent wood may be chopped and immersed in a lower alcohol such as ethanol, methanol, propanol or butanol or an aqueous solution of alcohol, and then extracted.

If the composition for improving atopic dermatitis of the present invention includes the extract of the essential oil of the fluency tree, the formulation is not particularly limited, the formulation of the cosmetic skin lotion (cosmetic water), nutrition lotion, nutrition cream, massage cream, face wash foam, It can be manufactured in various kinds such as face-washing water, pack or baby powder, and it contains skin extracts (nutrition lotion), nutrition lotion, nutrition cream, massage cream, face wash foam, and face wash while containing essential oil extract of changchang wood as an active ingredient. Conventional methods that may be applied in the preparation of water, packs or baby powders, or may be applied to the human body according to its efficacy may be applied as a medicament, ie, ointment.

Preferably in the composition for improving atopic dermatitis, the content of the extract of essential oil component of lychee tree is 0.1 to 30% by weight, and if it contains too small an amount out of the above range, the therapeutic and prophylactic effect of the skin of atopy is insignificant. If it contains a large amount, skin trouble may occur.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

Experimental Example

Example 1

Steam distillation was carried out as follows to obtain the extract from the fluent tree. First, the leaves, stems or wood flour were collected and chopped, and then put into 2 L and 5 L flasks, and 500 ml to 1 L of distilled water was added thereto, and the resultant was heated for 7 to 9 hours using a Karlsruker apparatus. After that, the essential oil component of the upper layer was collected using diethyl ether, and separated by defiltration with anhydrous sodium sulfate. In addition, the liquid extract from which the essential oil component of the upper layer was separated was distilled under reduced pressure at 40 or less to remove the solvent and concentrated to prepare an essential oil extract of lychee tree.

Example 2

300 g of leaves, stems or wood flour of lychee tree were immersed and extracted in 1 Kg of 40% ethanol aqueous solution for 3 days, and then filtered to prepare 500 g of lychee tree essential oil extract.

The essential oil extract extracted by the above procedure was tested for the effect of atopic dermatitis on the extract of Ryuchangmok essential oil using a mouse.

[Experiment 1]

First, as shown in FIG. 1, the back of the Nc / Nga mouse, which is 5 to 6 weeks of age, is epilated cleanly and left for 24 hours to heal fine wounds on the skin after depilation.

Thereafter, 200 μl of a 1% DNCB solution (acetone: olive oil = 3: 1) is applied to the back area to proceed with the immunological procedure. After 4 days, 150 μl of 0.2% DNCB solution is applied to the back area 2-3 times a week. After 4 to 5 weeks of treatment, dermatitis is sufficiently induced to remove all the back skin and facilitate the penetration of the therapeutic drug.

At this time, if a new dermatitis is formed in this area and the scratching action is intensified, the DNCB treatment is stopped, the sensory evaluation is performed, the blood is precipitated, the blood cells are precipitated, and only serum is taken and stored at -7 ° C.

Immediately before the end of the DNCB treatment, the drug to be tested for treatment is dissolved in a suitable solvent (a solution in which the compound is completely dissolved and without skin irritation) at a desired concentration, and twice a day, one day after the end of the DNCB treatment, 5 Daily drug solution is applied topically to the back (150 μl).

On the 5th day of drug treatment, the sensory evaluation is performed, then blood is taken and the skin at the back is excised and stored in a 10% formalin solution. The blood precipitates the blood cells, and only serum is taken and stored frozen at -70 ° C.

2. Evaluation method

[Equality evaluation]

For dermatitis of NC / Nga mice, a visual evaluation method commonly used in atopic dermatitis was used. The visual evaluation result is the sum of the scores of each of the following five items. The endpoints are divided into erythema, itching and dry skin, edema and escoriation, erosion, and lichenification. Each of these items is scored as none (0), weak (1), severity (2), and severe (3). Therefore, you can get a score between at least 0 and at most 15. In general, when DNCB is used to cause dermatitis, it can be judged that the dermatitis peaked at about 12 to 13 points.

[ELISA] (Pharmingen: manufacturer's recommindation)

After dermatitis, the blood lgE is quantified by capturing the fluid from the eye mucosa using capillaries at intervals of about two weeks. Usually, when the disease is induced and the clinical score exceeds 6-7 points, the blood lgE concentration can be measured by diluting the blood about 200-500 times as the concentration of lgE exceeds 10 μl / ml. Therefore, 50 μl of blood volume is sufficient.

Capture Ab was 250 times uncoated in the coating buffer, and 50 µl each was added to a 96 well plate for ELISA (overnight incubation at 4 ° C.) and the wells were washed five times with PBST.

Block each well with 10 μl assay diluent at room temperature for 1 hour, while blocking standard soln (100/50/25 / 12.5 / 6.3 / 3.3 / 1.6 ng / ml, 4.15 μl lgE standard soln in 200 μl assay diluent ) And sample soln (sample 2 μl in 1 mL assay diluent). 50 μl Tlr of standard soln is added to each well, incubated for 2 hours at room temperature, and the wells are washed 5 times with PBST.

Then, 50 μl of a working detector (detection Ab + Av + HRP in assay diluent) was added to each well, then covered with a wrap, incubated for 1 hour at room temperature, washed 5 times with PBST, and 50 TMB substrates were added to each well. Add μl and incubate for 30 minutes at room temperature. At this time, 25 μl of stop soln was added thereto to stop the reaction, and the absorbance was measured at 450 nm.

[Tissue Staining and Observation]

a) Mouse skin tissue is biopsied 1.5 x 1.5cm 2 around the affected area around the back and fixed in 10% formaldehyde solution and stored at 4 ° C until tissue staining. Skin tissues were observed mainly on the overall condition of the skin, infiltration and degranulation of mast cells, and eosinophil infiltration. To this end, tissues were stained using haematoxylin-eosin staining, toludine blue staining, and eosin staining, and observed at a magnification of about 200 times on an optical microscope.

b) The thickness change of the epidermis is obtained by using the proportional expression compared with the length of 200 times grid. Three tissue sections were obtained from 6 to 8 mice in each group, and then the average of the epidermal thicknesses of the five sections was obtained from one section.

Again, these three values were averaged to be the average epidermal thickness of one mouse, and these values of 6. 8 each were averaged to the epidermal thickness of each group.

c) Mast cell infiltration and degranulation were obtained from each of three tissue sections from 6 to 8 mice in each group, and then counted the number of mast cells infiltrated into 5 sections from one section, It is classified into three groups and then averaged. Again, these three values are averaged to determine the average value of one mouse, and these values of 6 to 8 animals are averaged to obtain mast cell infiltration values of each group.

d) Eosinophil infiltration is obtained by adding three tissue sections from each of 6 to 8 mice in each group and then adding the number of eosinophils shown in four fields in one section. The mean values of the three mice were averaged again to obtain the average value of one mouse, and these values of 6 to 8 animals were averaged to be the eosinophil infiltration value of each group.

[Data processing / evaluation method]

1) sensory evaluation

Sensory evaluation is performed twice at the end of DNCB treatment and at the end of treatment treatment. Take pictures and keep them after the medication is finished.

The results were evaluated by comparing the scores before and after the treatment with the scores before and after the treatment. Then, the results were compared with the reduction rates in the vehicle-treated group.

2) blood lgE concentration

Blood lgE concentrations are performed twice each at the end of DNCB treatment and at the end of treatment with the drug using the ELISA method. The resultant treatment was compared with the blood lgE concentration before and after the drug treatment to find the reduced rate, and then compared with the reduction rate of the vehicle treated group to verify the significance.

A. Drug treatment group: [lgE (drug) before treatment-lgE (drug) after treatment] / lgE (drug) before treatment

B. Vehicle treatment group: [lge (Vehicle) before treatment-lgE (Vehicle) after treatment] / lge (Vehicle) before treatment

3) biopsy

The biopsy method was used to perform biopsy once after the treatment drug treatment, and the results were significant compared to the vehicle treated group, such as epidermal thickness, mast cell infiltration and eosinophil infiltration. Compare with the decrease.

Experiment result

According to the above experiment, as shown in the state of the mouse shown in Figs. 2 to 4 attached to each experimental group, the result is better than the general atopic improved state results of the positive control treatment as shown in the table shown in FIG. It can be seen that the condition of mice treated with the essential oil extract of changwoo wood improved. As a result, skin cell constituent cell changes were improved in a) toluidine blue b) H & E (hematoxylin & eosin) as shown in FIG. can see.

According to the experiments and results as described above, the extract extracted from the leaves, stems or wood meal of the lychee tree according to the present invention can be prepared in a variety of formulations with a certain ratio.

Hereinafter, as an example of the formulation of the present invention, supple cosmetics (skin), nourishing cosmetics (lotion), essences, nutrition creams, massage creams, packs, face washes (cleansing foams), soaps, but includes the cosmetic composition of the present invention Formulation is not limited thereto.

Formulation 1.Flexible Lotion (Skin)

In order to prepare a flexible skin lotion for acne skin containing the lychee oil of the present invention, it may be blended as described in Table 1, and may be prepared according to a conventional manufacturing method in the cosmetic field.

TABLE 1

ingredient Content (% by weight) Rhodiola oil 0.1 to 30% 1,3-butylene glycol 3.0 glycerin 5.0 Polyoxyethylene (60) hardening castor oil 0.2 ethanol 8.0 Citric acid 0.02 Sodium citrate 0.06 antiseptic a very small amount Spices a very small amount Purified water To 100

Formulation Example 2. Nutritional Lotion (Lotion)

In order to produce a nourishing lotion for acne skin containing the lychee oil of the present invention, it can be formulated according to the preparation method in the conventional cosmetic field as shown in Table 2 below.

TABLE 2

ingredient Content (% by weight) Rhodiola oil 0.1 to 30% 1,3-butylene glycol 8.0 glycerin 5.0 Squalane 10.0 Monooleic acid polyoxyethylene sorbitan 2.0 Triethanol amine 1.5 Glyceryl Stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxy Vinyl Polymer 0.1 Arginine 0.1 antiseptic a very small amount Spices a very small amount Purified water To 100

Formulation Example 3. Essence

In order to prepare an essence for acne skin containing the fluency oil of the present invention, it may be formulated according to the preparation method in the conventional cosmetic field as shown in Table 3 below.

TABLE 3

ingredient Content (% by weight) Rhodiola oil 0.1 to 30% Cytostolol 1.7 Polyglyceryl 2-oleate 1.5 Ceramide 0.7 Ceteares-4 1.2 cholesterol 1.5 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Carboxy Vinyl Polymer 0.2 Xanthan Gum 0.2 antiseptic a very small amount Spices a very small amount Purified water To 100

Formulation Example 4 Facial Cleanser (Cleansing Foam)

To prepare acne skin cleansing agent (cleansing foam) comprising the fluency oil of the present invention, it may be combined as described in Table 4 below, and may be prepared according to a manufacturing method in a conventional cosmetic field.

TABLE 4

ingredient Content (% by weight) Rhodiola oil 0.1 to 30% N-acyl glutamate 20.0 glycerin 10.0 PEG-400 15.0 Propylene glycol 10.0 POE (15) oleyl alcohol ether 3.0 Lauric derivatives 2.0 Methyl paraben 0.2 EDTA-4Na 0.03 Spices 0.2 Purified water To 100

Formulation Example 5 Preparation of Nutritional Cream

To prepare a nourishing cream for acne skin containing the lychee oil of the present invention, it is prepared according to the preparation method in the conventional cosmetic field as described in Table 5 below.

TABLE 5

Ingredient Content (% by weight) Rhodiola oil 0.1 to 30% vaseline 7.0 Liquid paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.5 Sorbitan sesquioleate 1.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Xanthan Gum 0.5 Tocophenyl Acetate 0.1 Incense, preservative a very small amount Purified water To 100

      Formulation Example 6 Massage Cream Preparation

To prepare a massage cream for acne skin containing the lychee oil of the present invention, it is prepared according to the manufacturing method in the conventional cosmetic field as shown in Table 6 below.

TABLE 6

Ingredient Content (% by weight) Rhodiola oil 0.1 to 30% Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocophenyl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Xanthan Gum 0.5 Incense, preservative a very small amount Purified water To 100

      Formulation Example 7 Pack Preparation

It is prepared according to the manufacturing method in the general cosmetic field as described in Table 7 to prepare a pack for acne skin containing the fluency oil of the present invention.

TABLE 7

Ingredient Content (% by weight) Rhodiola oil 0.1 to 30% Propylene glycol 2.0 glycerin 4.0 Polyvinyl alcohol 10.0 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Incense, preservative a very small amount Purified water To 100

The present invention is not limited to the embodiments described above, and can be variously modified and changed by those skilled in the art, and can be applied to cosmetics of various uses including other color cosmetics, It can be used in the manufacture of a medicament that can be applied thinly applied to the human body, that is, ointment, which is included in the spirit and scope of the present invention as defined in the appended claims.

1 is a Nc / Nga experimental mice 5 to 6 weeks old according to the present invention

FIG. 2 is a diagram showing the mouse state of the result of DNCB treatment (21 days in total) of the mouse of FIG. 1.

3 is a diagram showing the mouse state of the result of positive control (total 28 days) by the combination treatment of Demacot S and hydrocortisone (100mg each) of the mouse of FIG.

Figure 4 is a view showing the mouse state of the result of treatment of the essential oil components of G.officinale (G.officinale) for 10 days of the present invention (total 28 days)

5 is a diagram showing the results of dermatitis symptoms according to the experiment of FIGS.

Figure 6 is a diagram showing the measurement results of the skin tissue composition changes according to the experiment of the essential oil extract of changchang wood according to the present invention

Claims (4)

Atopic skin improvement composition comprising an essential oil extract extracted from the leaves, stems or wood flour as an active ingredient. The method of claim 1, The composition is an atopic skin improvement composition, characterized in that to improve atopic skin through antibacterial, antifungal, free radical scavenging, anti-inflammatory and moisturizing action. The method of claim 1, A composition for improving atopic dermatitis, wherein the content of the lychee tree extract is 0.1 to 30% by weight in the composition. The method of claim 1, wherein Formulation of the cosmetic composition is a skin lotion (cosmetics), nutrition lotion, nutrition cream, massage cream, face wash foam, face wash, pack or baby powder, characterized in that the composition for improving atopic skin.

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