KR101182209B1 - Antimicrobial composition containing cinnamomum japonicum essential oil extract - Google Patents

Abstract

PURPOSE: An antibacterial composition containing Cinnamomum japonicum essential oil is provided to ensure antibacterial activity against Malassezia pachydermatis, Malassezia furfur, and Candida albicans; and to be applied in skin formulation and cosmetic materials for external use. CONSTITUTION: An antibacterial composition against Candida albicans contains Cinnamomum japonicum essential oil as an active ingredient. The essential oil contains 25-40 wt% of cineole, 10-30 wt% of cymene, 1-10 wt% of linalool, and 0.5-5 wt% of terpineol. The antibacterial composition is manufactured in the form of a solution, a suspension, a spray, a patch, a pad, a cream, an ointment, and a gel.

Description

Antimicrobial composition comprising essential oil extract essential oil extract {Antimicrobial composition containing Cinnamomum japonicum essential oil extract}

The present invention relates to an antimicrobial composition comprising a wild tree extract essential oil.

Cinamomum japonicum ) is a camphor family distributed in China, Taiwan, and Japan. It grows up to 1,100m above sea level in Jeju Island in Korea and grows in coastal and foothills in warm southern island. It grows to around 15m and reaches 30 ~ 40cm in diameter, and it can be exposed to strong light when it is young water or tree, and grows well in fertile soil with moisture. Cold and dry resistance is weak, but flame resistance and pollution resistance is strong and growth is relatively fast. In oriental medicine, the bark and fruit is used as a medicinal herb, which is mainly used to improve digestion of stomach and vomiting, dysentery and limbs. The leaves are rich in fragrance, so they are used as a substitute for tea or bath, and the fruit extracts confectionery oil. Wood is yellowish white or pale yellow brown industrial material (wood) with unclear distinction between sapwood and heartwood, and its structure is dense and used for building materials and furniture materials. Bark is called 계 (cinnamon), and fruit is called 계 子 (Field). do. The bark contains about 1% essential oil, its components are phellandrene, eugenol and methyleugenol, and the leaves contain about 1% essential oil, and its components are about 60% safrole, about 3% eugenol, 1,8 -cineol and the like. The drug has the effect of warming the stomach, causing acidity, and blood circulation. Abdominal cold chest pain, Hari abdominal pain, vomiting blow, customs pain pain, vaginal hand pain, bladder Hansan, ulcer , Pain (疼痛), such as hemoglobin (血痢 腸 風) to treat.

Essential oils are low-boiling oily substances stored in a specific space outside the cells differentiated from the epidermis or foliar tissue of the plant. It is an essential secondary metabolite for. Essential oils, which represent the unique aromas and flavors of plants, have been used since ancient times in the manufacture of food because of their aromas and their antiseptic properties, and have been applied in a wide variety of industries, such as perfumes, spices and pharmaceuticals. Until the Second World War, natural essential oils were used, but the development of the chemical industry increased the use of synthetic fragrances, reducing the use of plant essential oils. However, since the mid-1980s, there has been an increase in living standards, increased interest in health, problems with chemical processes, environmental pollution, and side effects of synthetic materials. Is increasing. In addition, as the antimicrobial activity of the essential oil, antioxidant effect, anticancer effect, sedation and other effects are known a lot of attention has been focused. Essential oils, which were mostly used as perfumes or giddys, are used in the manufacture of daily necessities such as cosmetics, soaps, and toothpastes. Recently, they are used in aromatherapy.

Since the 1970s, environmental pollution has sharpened and end-users' awareness of environmentally friendly products has been increasing since 2000, and regulations and monitoring of environmental products have been strengthened at the level of government and social organizations. It is expected to contribute to national health contribution, import substitution effect and improvement of forest related industry as a future-oriented industry through the application of wood natural oil as a clean natural material in various fields. In terms of foreign industrial aspects, Japan has developed antibacterial and deodorant fibers, plastics, filters, pigments, paints, and purifiers using essential oils, while Japan Oil & Technology Co., Ltd. has developed deodorants using quinones from blind bamboo. However, natural products are used in extremely small quantities. Most antimicrobial agents use synthetic fungicides, ceramics, silver salts, and the like. France crushes forest litter residues to produce 300 tons of phytoncide annually, earning 9 million francs of foreign currency, and Robert and Biorang (the company) occupy the world market, maximizing profits by selling licenses to 25 countries. .

Skin is the body's primary protective barrier and has a unique function of protecting internal organs from changes in temperature and humidity, and stimulation of the external environment such as ultraviolet rays and pollutants. However, the skin has an environment suitable for the growth of bacteria. As a result, the skin, which was sterile at the time of birth, receives bacterial contamination immediately after birth, and various bacteria are present. It is more than 100 years ago that bacteria on the surface of the skin began to be the cause of atopic dermatitis. In particular, Gram-positive cocci were considered to be the cause of eczema, a key symptom of dermatitis, and Staphylococcus aureus is a representative example. However, fungi present in normal skin as well as bacteria are known to affect the development of atopic dermatitis. If the skin barrier is damaged by scratching the skin due to atopic dermatitis, fungi can penetrate through the skin and cause an immune response, which can act as an exacerbation factor for atopic dermatitis.

According to the research team of Professor Nam Dong-ho of Ajou University Hospital, Candida albicans acts as an allergen in severe atopic dermatitis rather than house dust mite. The research team studied 523 patients with atopic dermatitis from February 2008 to September last year for two types of house dust mites and three types of fungi present in the skin (Malacesia, Candida, and Bacillus pneumoniae). As a result, 75% (394) of the patients showed strong positive responses to any one or more of them, of which 344 were positive for house dust mites and 298 were mildly positive. . Among allergens, 23% (120 patients) had higher levels of allergic fungi than home dust mites. Among fungi, people with allergies to Candida fungi were three times more likely to have severe atopic dermatitis than those without fungi.

Candida is a genus of incomplete bacillus fungi, which are spherical or oval in unicellular form, and are present in the mouth and skin of humans or animals, which are harmless to the human body under normal conditions. When this weakness, the abnormal growth in the body causes candidiasis. In areas with high frequency of infection, itching and pain occur in the mucous membranes of the mouth and genital area.

According to Professor Park Kyung-hwa of the Department of Infectious Medicine, Chonnam University School of Medicine, the number of cases of candidiasis in Korea is 1.3, which is not much lower than that of 1.5 in the United States. It is emerging. According to a study analyzing 639 Candida strains collected from 20 institutions in Korea, 38% of Candida albicans (C. albicans), 26% of Candida parapsilosis (C. parapsilosis) and Candida Tropicalis (C. tropicalis) 20%, and 11% of Candida glabrata resistant to fluconazole.

Malassezia (Pityrosporum ovale) is a lipid-friendly bacterium that belongs to the normal flora of the skin and naturally exists on the surface of the skin. Form and survive. Colonization by Malassezia is known to occur most often from late puberty to early adult, when the sebaceous glands are active immediately after birth. Malassezia eats fat and protein from the skin, which is highly lipase-active, secretes proinflammatory free fatty acids and increases the production of inflammatory cytokine, resulting in pityrosporum folliculitis, pityriasis versicolor, and seborrhea. Causes of derboritis dermatitis and dandruff.

Steroids, anti-inflammatory drugs and antimicrobial antibiotics are used as drugs to treat them. However, these drugs are toxic and long-term use may cause developmental problems in children. Diabetes, hypertension, or disease may worsen even in adults. There are various side effects such as lumbar, trunk enlargement, skin ancestry, clinical findings of hirsutism and electrolyte and water metabolic disorders. Currently, antifungal agents are mainly obtained from molds or chemically synthesized. Among them, chemically synthesized antifungal agents include polyene and azole derivatives, and arylamine-thiocarbamates. Recently developed azole compounds (ketoconazole, itraconazole, etc.) have been reported to have less serious toxicity, but side effects that inhibit the synthesis of steroid hormones in the endocrine system by inhibiting enzymes that depend on cytochrome P-450 have been reported. Development of Technology for Antibacterial, Antifungal and Hair Growth Functional Substances, Seoul National University, 2007].

Thus, the present inventors have identified the antimicrobial activity against fungi causing dermatitis, and confirmed that the essential oil extracts of the wild tree extract have antimicrobial activity against fungi causing dermatitis while studying natural antimicrobial agents that are safe from the side effects of chemical synthetic drugs. The invention was completed.

Accordingly, an object of the present invention is to provide an antimicrobial composition comprising essential oil extracted from wild wood.

Another object of the present invention is to provide an external preparation for skin including essential oil extracted essential oil.

In addition, the present invention has another object to provide a cosmetic comprising a wild tree extract essential oil.

As a means for solving the above problems, the present invention provides an antimicrobial composition comprising a wild tree extract essential oil.

In addition, as another means for solving the above problems, the present invention provides a skin external preparation including a wild tree extract essential oil.

In addition, as another means for solving the above problems, the present invention provides a cosmetic comprising a wild tree extract essential oil.

The present invention relates to an antimicrobial composition that is safe from the side effects of chemical synthetic drugs through the verification of the antimicrobial activity against fungi that cause dermatitis by extracting natural substances from the bark.

Figure 1 shows a natural fragrance (essential oil) extraction method through the steam distillation method.
Figure 2 shows the extraction process of essential oil extracted from wild wood.
Figure 3 shows the crude oil extracted essential oil by the solvent extraction method.
Figure 4 is a chromatographic result of analyzing the fragrance components of the essential oil extracted from wild wood.
Figure 5 shows the main component structure of the wild essential oil extracted essential oil.
Figure 6 confirms the antimicrobial activity against the causative agent of dermatitis of the bark extract.

The present invention relates to an antimicrobial composition comprising a wild rose extract essential oil as an active ingredient.

The raw bark extract of the present invention, preferably the essential oil component, can be extracted using a general extraction method known in the art, or by distillation extraction or solvent extraction:

Steam distillation includes water distillation, in which plants are put directly into the water and applied heat, steam distillation, which is extracted by injecting hot steam on a shelf or sieve, and water and steam distillation, both of which are combined. The principle is that when hot heat is applied, the cells containing essential oils are destroyed in the plant, distilled with steam, and the oil-soluble essential oils and water-soluble water vapors are separated while passing through a cooler to extract only essential oils.

Solvent Extraction (Solvent Extraction) is a method mainly used when it is difficult to extract the steam distillation with a very small amount of fragrance, and has a disadvantage that the wax component rather than volatile components can be extracted compared to the fragrance extracted by distillation. . There are various kinds of solvents such as petroleum ether, hexane and alcohol (preferably methanol, ethanol). Recently, carbon dioxide is used, and animal and vegetable oils are also used. This method was first applied by Robiquet and has been widely used today as a modern method by Buchner and Favrot, which improved the equipment, solvents and operating methods. The operation is performed at room temperature by putting fresh ingredients into an extraction apparatus and adding a solvent to extract the fragrance. It is possible to extract without changing the oil component by the soft extraction method, and the extract is transferred to an evaporator such as a rotary concentrator, concentrated at low temperature, and decompressed to completely remove the solvent, which is called concrete (concrete oil). The concrete contains some wax, protein, and pigments. Absolute can be obtained by shaking in high concentrations of alcohol, filtration and cooling again to remove the insoluble wax.

Birch bark extract essential oil of the present invention contains 25 to 40% by weight of the cineol, 10 to 30% by weight of cimenol, 1 to 10% by weight of linarol and 0.5 to 5% by weight of terpineol.

Among the composition components, cineol is a colorless liquid natural organic compound, and fresh cineol is a colorless liquid natural organic compound. It has a fresh camphor odor, aroma, and a cool taste. It contains more than 90% of eucalyptus vegetable fragrance oil. It is contained in bay leaves, mugwort, hibiscus, rosemary, sage, and aromatic plant leaves. It is used as a spice, perfume, and cosmetic. It is valuable as a treatment for chronic obstructive pulmonary disease and asthma. It is effective in relieving inflammation and pain and kills leukemia cells. It is also used as a suppository to treat respiratory anxiety, and is known to be an effective treatment for vaginal sinusitis that has not been removed in a temporarily suppressed experiment. Ximen is a compound with a methyl and arisopropyl group bonded to the benzene nucleus and has a floral scent. Linarool is a chain-shaped monoterpene alcohol that exists in natural products and has the same direction as lily of the valley, aloe, etc. It is used as a fragrance. Terpineol is a lilac-scented liquid found in orange oil, cedar leaf essential oil, cypress essential oil, and pine and plant leaves.

The wild rose extract essential oil is Malassezia , known as the causative agent of dermatitis pachydermatis ), Malassezia furfur ) and candida albicans ( Candida albicans) has an excellent antimicrobial effect can be confirmed by the disk diffusion method.

Therefore, the rhododendron extract essential oil of the present invention is excellent in antimicrobial effect, there is no side effect can be usefully used as an antimicrobial composition, skin external preparation, cosmetics.

The essential oil extract is essential oil to remove harmful bacteria and fungi is not particularly limited in the formulation, at least one active ingredient exhibiting the same or similar function in addition to the essential oil extracted essential oil. It may contain.

The antimicrobial composition may be an external preparation for skin, and specifically, may be prepared as a solution, a suspension, a spray, a patch, a pad, a cream, an ointment, or a gel.

In addition to the active ingredients, the solution may be prepared by conventional excipients such as solvents, solubilizers and emulsifiers such as water, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, dimethylformamide, oils, especially cottonseed oil, peanut oil, camellia oil, aloe vera, glycerin, natural corn seed oil, olive oil, castor oil, almond oil and sesame oil, glycerol, glycerol form alcohol, tetrahydrofur Fatty acid esters of furyl alcohol, polyethylene glycol and sorbitan, or mixtures of these materials, and may include methyl or propyl-p-hydroxybenzoate or sorbic acid, and the like as preservatives.

Suspending agents, in addition to active ingredients, include conventional excipients, for example liquid diluents such as water, ethyl alcohol and propylene glycol, polyethylene glycol, and suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol, sorbitan Esters, cellulose derivatives and hydrogenated edible fats and oils), microcrystalline cellulose, aluminum metahydroxide, bentonite, agar and tragacanth, injectable esters such as ethyl oleate or mixtures of these materials.

Ointments, creams and gels may be used in addition to the active compounds, including conventional excipients such as animal and vegetable fats, wax paraffins, starches, targacantes, cellulose derivatives, polyethylene glycols, silicones, bentonite, silicic acid, talc and zinc oxide or these substances It may contain a mixture of.

In addition, the composition may be a cosmetic composition, it is not particularly limited in the formulation may have a conventional formulation. Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Essence, Nutrition Essence, Pack, Liquid or Solid Soap, Shampoo, Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser and bathing agent.

The cosmetic composition may include fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or Nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives or auxiliaries commonly used in the field of dermatology such as lipid vesicles It may contain. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

Based on in vitro and in vivo experiments, the extract from the wild tree is preferably 0.00001 to 50 parts by weight, more preferably 0.0001 to 10 parts by weight, most preferably to the total weight of the antimicrobial composition of the present invention. 0.001 to 0.1 parts by weight may be included.

Hereinafter, the present invention will be described in detail by examples and production examples.

However, the following Examples and Preparation Examples are merely illustrative of the present invention, the contents of the present invention is not limited by the following Examples.

Example 1 Extraction and Yield Analysis of Wild Tree Essential Oil

Extracted from Wando Arboretum in August 2010, the leaves and stems were separated, washed with water, and dried hawthorn was extracted by solvent extraction.

500 g of dalberry was crushed with a grinder, soaked in about 3 L of 6-fold hexane (n-Hexane, 95%), extracted for 24 hours, filtered with Whatman No.2 filter paper, and concentrated under reduced pressure at 40 ° C. Removal gave concrete oil (yield 1.2%) [FIGS. 2 and 3].

Example 2: Analysis of Essential Oil Fragrance Extract

The essential oil obtained by solvent extraction in Example 1 was analyzed by fragrance component composition by gas chromatography mass spectrometer (GCMS-QP2010 Plus, Shimadzu).

Gas Chromatography Mass Spectrometry (GC / MS) Analysis Conditions GC / MS
Column
Carrier gas
Temperature

-Column Oven
-Injection
-Ion source
Interface
Detector voltage SHIMADZU, GCMS-QP2010 Plus
Rtx-5MS (60m × 0.25mm × 0.25㎛)
He: 1.7ml / min

(5 ℃ / min)
35 ° C (1min) -----------> 250 ° C (5min)
250 ℃
200 ℃
250 ℃
1.2 kV

Composition of Flavor Components in Birch Concrete Oil division R. time Area Area % compound One 5.332 3,358,785 0.77 Methyl cyclopentane 2 5.926 3,915,049 0.90 Benzene hexahydride 3 11.179 5,418,751 1.25 3-Hexen-1-ol 4 11.570 3,897,193 0.90 1-Hexanol 5 13.520 507,559 0.12 alpha-Thujen 6 13.795 2,751,397 0.63 alpha-pinene 7 15.126 5,843,513 1.35 (+)-sabinene 8 15.287 2,830,082 0.65 (+)-Beta-pinene 9 15.587 7,472,121 1.72 Beta-Myrcene 10 16.147 14,489,252 3.34 alpha-phellandren 11 16.579 2,335,751 0.54 alpha-Terpine 12 16.814 89,744,927 20.66 cymene, cymol 13 17.006 13,122,114 3.02 beta-Phellandren 14 17.125 139,304,232 32.07 1,8-cineol 15 17.472 450,286 0.10 (3E) -3,7-Dimethyl-1,3,6-octatriene 16 17.927 2,935,940 0.68 Gamma-terpinene 17 18.295 1,359,870 0.31 4-Thujanol 18 18.940 1,769,339 0.41 cis-D-Dihydrocarveol 19 19.171 18,424,458 4.24 (+-)-Linalool 20 19.325 705,060 0.16 4-Thujanol 21 19.849 2,402,100 0.55 Benzen ethanol 22 20.522 938,030 0.22 Cyclopentasiloxane, decamethyl 23 20.962 1,538,515 0.35 2,6-Dimethylocta-1,5,7 trien-3-ol 24 21.472 2,952,871 0.68 2,6-Dimethylocta-1,5,7 trien-3-ol 25 21.797 9,235,952 2.13 4-terpineol 26 21.968 1,505,261 0.35 1-Methyl-4- (alpha-hydroxyisopropyl) benzene 27 22.178 42,874,192 9.87 2- (4-Methyl-3-cyclohexen-1-YL) 2-propanol 28 22.555 5,922,193 1.36 A-Phellandrene epoxid 29 23.576 4,327,115 1.00  A-Phellandrene epoxid 30 25.511 1,040,254 0.24 2,2,4,4,6,6,8,8,10,10,12,12-Dodecamethylcyclohexasiloxane 31 27.388 4,255,944 0.98 Alpha-copaen 32 27.511 14,432,158 3.32 Alpha-copaen 33 27.861 1,295,248 0.30 8-Isopropyl-1-methyl-5-methylene-1,6-cyclodecadiene 34 28.383 1,418,493 0.33 3- (Hydroxymethyl) -6-isopropyl-2-cyclohexen-1-one 35 28.506 2,714,870 0.63 Aromadendrene 36 28.785 8,266,676 1.90 2,6,10,10-Tetramethylbicyclo (7,2,0) undeca-1,6-diene 37 29.667 723,168 0.17 Alpha-caryophyllene (Alpha-humulene) 38 29.883 3,306,449 0.76 Aromadendrene 39 - 9,523,007 1.05 unknown total 434328332 100.00

As a result of analyzing the fragrance components of the bark of the wild tree extract, the main fragrance components were 32.1% cineol, 20.7% cymene, 9.9% 2- (4-methyl-3-cyclohexene-1-YL) 2-propanol, 4.2% linalool, 3.3% α-phellandrene, 3.3% α-copan, 3% β-phellandrene, 2% 4-terpineol. In addition, 1.9% Tedramethylbicyclo undecadiene, 1.7% β-myrcene, 1.4% A-phellandrene epoxide, 1.4% (+)-sabinene and 1-hexanol, aromadendrene, γ-ter It consists of trace components, such as pinene, (beta) -pinene, (alpha) -pinene, (alpha) -terpine, and 4-tuzene (FIGS. 4 and 5).

Example 3: Determination of antimicrobial activity against the causative agent of dermatitis

The harmful bacteria used in the experiment were Malassezia pachydermatis (ATCC14522), Malassezia a furfur, ATCC14521), Candida albicans (Candida albicans, ATCC10259) were used. Three food poisoning bacteria were inoculated in Nutrient Broth (Difco 234000) liquid medium and incubated for 24 to 48 hours at 37 ± 1 ℃, and Malassezia fascimatis was treated with Saboruraud's glucose broth (Difco 0382) and Malassezia purpur. Inoculated in YM broth (Difco 0711) with% olive oil was incubated for 24 to 48 hours at 37 ± 1 ℃. Candida albicans was inoculated in YM broth (Difco 0711) and enriched for 24 to 48 hours at 24 ± 1 ° C.

The antimicrobial activity of the extract was measured by the disc diffusion method. The extracted fragrance was dissolved in ethanol (99%) at a concentration of 10 mg / 100 μl, absorbed by 50 μl in sterile paper disc (Toyo, 8mm, Japan) and dried to remove all solvents.

Dilute the liquid broth at 600 nm wavelength so that the absorbance is 0.3 ~ 0.5, and inoculate 0.1 mL of each plate on the plate medium, and put the paper disk on which the sample is absorbed and put it on the plate coated with bacteria. Incubation at 24 ° C. for 48 hours was performed to measure the diameter (mm) of the inhibition zone generated around the paper disc.

As a result, the extract showed a particularly strong antimicrobial activity against Candida albicans, Malassezia fascimatis, Malassezia purpuro bacteria causing dermatitis [Fig. 6].

Results of Determination of Antimicrobial Activity of Extracts by Trees division Cypress Cedar Birch Tree Brushwood camphor tree dermatitis
Causative bacteria C. albicans - - 36 - + M. pachydermatis - - *Measure
Impossible + + M. furfur - - + +

  -: Does not show antimicrobial activity,

  +: Diameter measurement is not possible but shows inhibitory activity,

*: Bacteria cannot grow due to strong antibacterial activity

Formulation Example 1 Preparation of Ointment

The ointment was prepared by a conventional method according to the following composition.

54.1 parts by weight of purified water

Glycerin 8.0 parts by weight

Butylene glycol 4.0 parts by weight

15.0 parts by weight of liquid paraffin

Beta glucan 7.0 parts by weight

Carbomer 0.1 parts by weight

Birch Extract Essential Oil 1.0 parts by weight

Caprylic / Capric Triglyceride 3.0 parts by weight

Squalane 1.0 parts by weight

Cetearyl Glucoside 1.5 parts by weight

Sorbitan stearate 0.4 parts by weight

Cetearyl Alcohol 1.0 part by weight

Beeswax 4.0 parts by weight

Formulation Example 2: Lotion Preparation

The lotion was prepared by a conventional method according to the following composition.

0.1 ml by weight of essential oil extracted

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

51.7 parts by weight of purified water

Claims (8)

Candida albi containing essential oil of Cinamomum japonicum , which contains 25 to 40% by weight of cineol, 10 to 30% by weight of cymenol, 1 to 10% by weight of linarol, and 0.5 to 5% by weight of terpineol. Antimicrobial composition against Candida albicans . delete delete delete delete delete delete delete

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