KR101575722B1 - Composition comprising the cornus officinalis extract for anti-oxidant, anti-bacterial, anti-inflammatory effect - Google Patents

Abstract

The present invention relates to an antioxidant, antibacterial or anti-inflammatory composition containing a corn oil extract.
According to the present invention as described above, it is possible to improve the skin diseases such as acne, atopy, athlete's foot, psoriasis, eczema or dermatitis by using a composition having an antioxidant, antibacterial and anti-inflammatory effect, .

Description

FIELD OF THE INVENTION [0001] The present invention relates to antioxidant, antimicrobial and anti-inflammatory compositions containing an extract of corn oil,

The present invention relates to an antioxidant, antibacterial or anti-inflammatory composition containing a corn oil extract.

Acne occurs in most people of all ages and is a skin disease that forms jaundice or pustule. The cause of acne is caused by complex factors such as excessive sebum secretion, hormone imbalance, abnormal keratinization in hair follicle, proliferation of Propionibacterium acnes and inflammatory reaction. P. acnes , a causative agent of acne, is an anaerobic skin fungus that proliferates in the hair follicle, and triglycerides of sebum are produced by free radicals produced by hydrolysis of lipase, and by the stimulation of the hair follicles by the chemotactic factor, Reaction. The treatment of acne caused by P. acnes infection is most commonly used with antibiotics, but its effectiveness may be deteriorated due to resistance to antibiotics. Therefore, studies on herbal medicine-derived substances for the treatment of acne have been actively conducted.

Recently, various cosmetic products using natural products have been developed to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin but also have a high level of consumer value, which is a development value as a raw material for cosmetics.

Korean Patent No. 10-1253374 (Cosmetic composition for improving acne skin) is a cosmetic composition for improving acne skin using fermented extract of Kimchi lactic acid bacteria and fermented soybean extract, and Korean Patent No. 10-0879032 (natural antibacterial and anti-inflammatory Antimicrobial, antioxidant and antiinflammatory agents having an effect and a cosmetic composition containing the same) provide a cosmetic composition having antimicrobial effect by using the herbal medicine licorice, zymo, chewyum, yellow whites, have.

It is a medicinal plant belonging to the Comaceae family. Its fruit is a circular shape with a length of about 1.5 cm. It is widely used as a natural food having a natural tonic effect. Mountainous oils include crystalline organic acids, gallic acid, malic acid, and wine acids, and iridoid glycosides such as moronic acid and roganin are known. According to the records of oriental medicine, it is known that corn oil is tasteful and warm in nature, and has been used as a treatment for diabetes, back pain, tinnitus, pulmonary tuberculosis and the like, and has pharmacological actions such as diuretic action, hypotensive action, and anticancer.

However, there are not many examples of application of corn oil as a cosmetic composition or a composition for external application for skin, and the effect of the corn oil on the fruit has been studied. Corn flour is almost toxic to the human body because it can be drunk by car. Also, the prior art discloses only the effect of the mixture of corn syrup mixed with other natural materials, and there is no example applied to the dermatitis such as acne and atopy by utilizing the effect of corn syrup itself.

Korean Patent No. 10-0361040 (a cosmetic composition containing a niozyme stabilized with yellow rose, rhubarb, rhubarb, passerine, angelica, clove and band extract) is an extract of yellow, white, Is effectively stabilized in the nyoxis, thereby improving the penetration of the skin, thereby promoting skin elasticity and providing a continuous moisturizing effect. The prior art is merely using a mixture of natural fruit with corn oil. Accordingly, the present inventors intend to provide a new use of the corn oil extract, furthermore the corn oil flower extract.

It is an object of the present invention to provide a novel use of a marine oil extract, which is a natural material without side effects, and to provide a composition having antioxidative, antibacterial and anti-inflammatory effects containing a marine oil extract as an effective ingredient.

In order to achieve the above object, the present invention provides an antioxidant, antibacterial or anti-inflammatory composition containing Cornus officinalis extract as an active ingredient.

Wherein the acidified milk extract is characterized in that it is a sour milk flower extract.

The mushroom oil extract is characterized by being extracted with a solvent extraction method, a supercritical extraction method, or an ultrasonic extraction method.

At this time, the solvent extraction method is a method in which the solvent extraction is carried out using water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, dipropylene glycol, glycerin, acetone, ethyl acetate, chloroform, methylene chloride, butyl acetate, diethyl ether, dichloromethane, And an extraction solvent selected from the group consisting of:

The extract of corn oil is characterized by containing 1 to 99% by weight of the antioxidant, antibacterial or anti-inflammatory composition.

The present invention also provides a composition for external application for skin containing the above antioxidant, antibacterial or anti-inflammatory composition.

The composition for external application for skin is characterized by improving any one of skin diseases selected from the group including acne, atopy, athlete's foot, psoriasis, eczema and dermatitis.

The composition for external application for skin is characterized by inhibiting the growth of Propionibacterium acnes or Staphylococcus epidermidis .

The composition for external application for skin is characterized in that it is formulated into an ointment, solution, cream, emulsion, lotion, lotion, gel, essence, foundation, pack, stick, bath or soap.

The present invention also provides a health functional food containing the antioxidant, antibacterial or anti-inflammatory composition.

According to the present invention, by containing a corn oil extract as an active ingredient, it provides a composition having excellent antioxidative, antibacterial and anti-inflammatory effects and is effective for improving skin diseases such as acne, atopy, athlete's foot, psoriasis, eczema or dermatitis .

The composition containing the extract of corn oil according to the present invention can be used not only for Propionibacterium acnes which is a cause of acne but also for Staphylococcus epidermidis which is a second skin infectious microorganism after acne infection, And excellent anti-inflammatory effect, which inhibits inflammation-related cytokine TNF-α in macrophages and suppresses NO production secreted by macrophages after LPS stimulation.

Fig. 1 shows a process for producing a corn oil extract.
Fig. 2 shows the results of antioxidant activity of DPPH extract of corn oil.
Fig. 3 shows the results of TNF-a inhibitory activity of the extract of Cornus syrup.
Fig. 4 shows the NO (nitric oxide) inhibitory activity of the extract of corn oil.

Hereinafter, the present invention will be described in detail.

The present invention provides an antioxidant, antibacterial or anti-inflammatory composition containing Cornus officinalis extract as an active ingredient.

It is preferable that the above-mentioned crude oil extract is a flower oil extract.

The crude oil extract may be extracted by a solvent extraction method, a supercritical extraction method or an ultrasonic extraction method, and may be extracted by cold extraction, hot water extraction, reflux cooling extraction or heat extraction. The solvent extraction method can be carried out in the presence of water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, dipropylene glycol, glycerin, acetone, ethyl acetate, chloroform, methylene chloride, butyl acetate, diethyl ether, dichloromethane and hexane It is preferable to use at least one extraction solvent selected from the group

More specifically, the extract of corn oil according to the present invention comprises (1) washing and drying the corn oil, the flower or the mixed material thereof; (2) a step of immersing the dried crude fermented milk in an extraction solvent to prepare a fermented milk extract; And (3) filtering and concentrating the prepared ferulic acid extract.

In the step (1), drying is preferably performed at 20 to 60 ° C for 1 to 2 days.

In the step (2), the extraction step may be carried out at a temperature of 10 to 20 times, preferably 10 to 15 times the weight of the raw materials of the acidic or unsaturated fatty acids, water, an anhydride or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, The reaction is carried out at a temperature of 5 to 100 ° C, preferably 20 to 100 ° C, more preferably 20 to 100 ° C, in the presence of an alcohol, glycol, glycerin, acetone, ethyl acetate, chloroform, methylene chloride, butyl acetate, diethyl ether, dichloromethane, Extraction at a temperature of 50 to 90 占 폚 for 12 to 36 hours shows the optimum effect. The extraction process is preferably repeated one to five times, and methanol, ethanol or butanol may be used as the lower alcohol.

The extract of corn oil is contained in an amount of 1 to 99% by weight, preferably 20 to 40% by weight, based on the antioxidant, antibacterial or anti-inflammatory composition.

The present invention also provides a composition for external application for skin containing the above antioxidant, antibacterial or anti-inflammatory composition.

The composition for external application for skin may be used as a cosmetic composition for improving skin and may be used for improving skin diseases selected from the group including acne, atopy, athlete's foot, psoriasis, eczema and dermatitis, There is an effect of preventing loss.

The composition for external application for skin has an effect of inhibiting the growth of Propionibacterium acnes or Staphylococcus epidermidis .

The present invention is based on the finding that the extract extracted from corn oil flowers has excellent antimicrobial activity against Staphylococcus epidermidis which is a secondary skin infectious bacterium after acne fungus infection as well as Propionibacterium acnes which is an acne cause bacteria It has an excellent anti-acne effect, and it has excellent anti-inflammatory effect which inhibits inflammation-related cytokine TNF-α in macrophages and suppresses secretion of NO secreted by macrophages after LPS stimulation. Antibiotics such as tetracycline have limitations in use such as side effects and the appearance of resistant bacteria, while the extract of Sanskrit flowers according to the present invention is safe and has no side effects and is suitable as a composition for external application for skin.

The composition for external application for skin may be formulated into an ointment, solution, cream, emulsion, lotion, lotion, gel, essence, foundation, pack, stick, bath or soap.

The present invention also provides a health functional food containing the antioxidant, antibacterial or anti-inflammatory composition.

Various formulation examples including the extract of corn oil of the present invention are described below, but the present invention is not intended to be limited but is to be specifically described.

Formulation Example 1. Flexible lotion (skin lotion)

Corn oil extract of the example of the present invention ..... 5.0

Glycerin ............................... 3.5

Oleyl alcohol ...................... 1.5

Ethanol ................................... 5.5

Polysorbate 80 ............... 3.2

Carboxyl vinyl polymer ............ 1.0

Butylene glycol ...................... 2.0

Propylene glycol .................. 2.0

Preservative, fragrance ........................

Purified water ................................... Remainder

Total .............................................. 100

Flexible lotion was prepared according to the above composition in a conventional manner (unit weight%).

Formulation Example 2. Nourishing lotion (milk lotion)

Corn oil extract of the example of the present invention 5.0

Glycerin ................................................ 3.0

Butylene glycol .......................................... 3.0

Propylene glycol ...................................... 3.0

Carboxyvinyl polymer 0.1

Wax ................................................. 4.0 ... 4.0

Polysorbate 60 ...................................... 1.5

Caprylic / Capriculiglyceride ........ 5.0

Squalane ................................................. ... 5.0

Solvatescequioleate .............................. 1.5

Cetearyl Alcohol ......................................... 1.0

Triethanolamine ............................................ 0.2

Preservatives, fragrances ............................................... Suitable amount

Purified water................................................. ......... Remaining

system................................................. ................... 100

Nutrition lotion was prepared according to the above composition in a conventional manner (unit weight%).

Formulation Example 3. Nourishing cream

Corn oil extract of the example of the present invention 3.0

glycerin................................................. .3.5

Butylene glycol ...............................................

Liquid paraffin ...............................................

Beta Glucan ................................................ 7.0

Cabomas ................................................ ......... 0.1

Caprylic / Capriculiglyceride ......... 3.0

Squalane ................................................. .... 5.0

Cetearyl Glucoside ........................... 1.5

Sorbitan stearate ........................... 0.4

Polysorbate 60 ......................................... 1.2

Triethanolamine ........................................... 0.1

Preservatives, fragrances ............................................... .. Good

Purified water................................................. ............ Remaining amount

system................................................. .................... 100

Nutritive creams were prepared according to the above composition in a conventional manner (unit weight%).

Formulation Example 4. Pack

The extract of corn oil of the Example 8.0 ... 8.0

Glycerin ..................................... 4.0

Polyvinyl alcohol ........................... 15.0

Hyaluronic Acid Extract 5.0

Beta Glucan ................................. 7.0

Allantoin ....................................... 0.1

Nonyl phenyl ether ......................... 0.4

Polysorbate 60 ............... 1.2

Preservative, fragrance ..............................

Purified water ......................................... Remaining amount

system................................................. ... 100

A pack was prepared according to the above composition in a conventional manner (unit weight%).

Formulation Example 5. Pharmaceutical preparation for local administration (patch)

The extract of corn oil of the example ....................................... 4.0

Beta-1,3-glucan ........................................... 3.0 3.0

Diethylamine ............................................... ........ 0.7

Sodium Sulfite ................................................ ..... 0.1

Polyoxyethylene lauryl ether (EO = 9)

Polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000) ......... 1.0

Viscous paraffin oil .............................................. .2.5

Caprylic acid ester / capric acid ester (Cetiol LC) 2.5

Polyethylene glycol 400 ............................................... 3.0

Polyacrylic acid (Carbopol 934P) ....................................... 1.0

Purified water................................................. .......... Remaining

system................................................. ............... 100

According to the above composition, a medicament for topical administration was prepared by a conventional method (unit% by weight).

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

EXAMPLES Example 1. Preparation of Corn oil extract

The crude oil flowers were washed once or twice with distilled water, and then dried at 20 to 60 ° C for 1 to 2 days to prepare an extraction raw material.

50 g of the prepared corn oil was dipped in 750 ml of distilled water, methanol and 70% ethanol, respectively, and extracted at 50 to 70 ° C for 12 to 36 hours. Thereafter, the insoluble component was washed with 1-2 times of water and naturally filtered with a filter paper having a pore size of 5 쨉 m or less, and then concentrated with a reduced pressure concentrator until a Brix of about 3 to 6 as measured by a sugar content meter Lyophilized and homogenized with a blender in the absence of moisture (Fig. 1).

The following experiment was carried out using the prepared extract of Sanskrit flowers.

Preparation Yes. Experimental strain and culture

In the experiment according to the present invention, Propionibacterium acnes KCTC3314, which is a causative agent of acne, and Staphylococcus epidermidis KTTC12228, which is a gram-positive skin preparation, were used.

P. acnes was inoculated into Reinforced clostridial (RC) medium, sealed in a gaspack system in an anaerobic incubator, anaerobically incubated at 37 ° C for 3 days, and aerobic strain S. epidermidis was inoculated on Mueller-Hinton medium And then cultured at 37 ° C for 24 hours.

Experimental Example 1. Antioxidant activity of the extract of corn oil

Antioxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picryl hydrazyl) method and L-ascorbic acid was used as a comparative substance. DPPH and L-ascorbic acid (Sigma) were used as a positive control. DPPH was a stable, relatively stable free radical which was reduced by the sample and decolorized. Therefore, DPPH is used to measure antioxidative activity.

The extracts were continuously diluted (1000ug-0ug) twice in 200 μl of 0.2 mM DPPH ethanol solution and reacted at room temperature for 30 minutes. Absorbance was measured at 517 nm. The free radical scavenging activity was determined using the following equation, and the electron donating ability was represented by the absorbance reduction rate of the addition group of the sample and the no addition group.

Free radical scavenging ability (%) = (1-absorbance of sample addition group / absorbance of no addition group) x 100

The free radical scavenging ability of the extract inhibitor, ie, the ability to reduce or counteract the free radical DPPH, ie, oxidation inhibition, was evaluated by comparing the antioxidant activity of the positive control L-ascorbic acid with that of the 70% EtOH extract In the case of MeOH extract, the antioxidant activity was about 85% or more at a treatment concentration of 31.25 ug / ml or more (FIG. 2). Therefore, it was confirmed that the extract of corn oil has an excellent antioxidative effect.

Experimental Example 2. Antimicrobial activity of the extract of corn oil

The antimicrobial activity was measured by the disk diffusion method.

The acne causative organism P. acnes was cultured in a Reinforced clostridial (RC) liquid medium for 3 days, and then the suspension of the bacteria was adjusted to a constant concentration (optical density at 620 nm, absorbance 0.5) on an RC agar plate using a sterile cotton swab. . After that, the plate was dried and placed on a plate surface (Φ 8 mm, Adventec. USA) inoculated with the strain to absorb the extract sample by concentration. The plate treated with the sample was incubated in the anaerobic incubator for 3 days using the gaspack system, and the antimicrobial activity was measured in the inhibition zone size around the disk.

The antimicrobial activity evaluation against the aerobic strain S. epidermidis was carried out in the same manner as the above-mentioned P. acnes except that the antimicrobial activity was cultured at 37 DEG C for 24 hours using Muller-Hinton medium.

As a result, when 1 mg of tetracycline as a positive control group was treated, the diameter of the inhibitory circle was about 12 mm. Thus, it can be seen that the extract of corn oil has excellent antimicrobial activity against both P. acnes and S. epidermidis. Antibiotics such as tetracycline have limitations in use such as side effects and appearance of resistant bacteria, while the extract of corn oil according to the present invention is safe and has no side effects and is suitable as a composition for external application for skin.

EXPERIMENTAL EXAMPLE 3 Cytotoxicity of Cornicola oil Extract

Human acute monocytic leukemia cells (THP-1) (human acute monocytic leukemia) cultured in RPMI-1640 medium without FBS were cultured in 96-well microplates at a concentration of 5 × 10 ⁴ cells / well. The supernatant was removed and the supernatant was removed. 100 μl of the MTT solution was added to the solution in a solution of 36 Lt; 0 > C for 4 hours. After removing the culture medium and treating with 50 μl of DMSO, the absorbance was measured with an absorbance meter (ELISA) at 540 nm and the measured value was calculated by the following formula.

Cytotoxicity (%) = (1-absorbance of sample-added group / absorbance of no-added group) × 100

Experimental Example 4. Anti-inflammatory activity of crude oil extract - TNF-α inhibition

Human acute monocytic leukemia cells (THP-1) (human acute monocytic leukemia) cultured in RPMI-1640 medium without FBS were cultured in 96-well microplates at a concentration of 5 × 10 ⁴ cells / well. The extracts were incubated for 24 hours, washed three times with FBS, and the extracts were pre - cultured for 2 hours. Lipopolysaccharide (LPS) was then treated for 6 hours. After centrifugation, the supernatant was dispensed into a TNF-α ELISA Kit (BD 555212) and absorbance was measured at 450 nm with an absorbance analyzer ELISA reader.

In the inflammatory response induced by LPS, the degree of inhibition of TNF-α, a typical cytokine, was measured. The amount of TNF- [alpha] secreted by the LPS-induced inflammatory reaction was measured and expressed as the percentage of TNF- [alpha] secreted in the extract-treated group when this value was taken as 100. The cytotoxicity of extracts was compared with that of extracts to determine whether the extract reduced TNF-α production induced by LPS or caused cell population deterioration due to cytotoxicity of the extract.

As a result, when the extracts were treated with cells at a concentration of 100 ug / ml, the survival rate of the cells was more than 95%, indicating that the extract of Cornus nipponensis did not affect the cell survival and inhibited TNF- (Fig. 3).

Experimental Example 5. Anti-inflammatory activity of oxalic acid extract-NO (nitric oxide) inhibition

Using a RAW 264.7 cell line, 200 μl aliquots were added to DMEM containing 10% (v / v) FBS, 100 units / ml penicillin and 100 ug / ml streptomycin at a density of 5 × 10 ⁴ cells / And cultured in a 5% CO ₂ incubator at 37 ° C for 6 hours. Then, LPS-added extract samples were added at different doses and cultured for 16 hours.

For NO analysis, the amount of nitrate produced during cell culture was measured. 150 μl of the cell culture supernatant was centrifuged, and 100 μl of the supernatant was reacted with 100 μl of Griess reagent for 5 minutes, and the absorbance was measured at 540 nm using an ELISA reader. At this time, the NO inhibitory activity of each treatment was compared with the relative amount of NO produced finally based on the standard NaNO ₂ , and the activity was determined as an index of anti-inflammatory activity. When the inflammatory reaction is induced, NO production is induced, which is measured by the greiss reagent after stabilizing nitrate to nitrate. The amount of NO produced in the group to which LPS alone was added was regarded as 100%, and the addition of the sample was evaluated in terms of%.

As a result, LPS did not inhibit NO because it was a control group that induced inflammation reaction, and normal cells without LPS treatment showed almost 100% NO inhibition effect because inflammation reaction was not induced. The NO inhibitory effect of each extract sample was increased to 50 ug-200 ug as the concentration increased. Treatment with 200 ug / ml of MeOH extract showed about 32% inhibition of NO (FIG. 4).

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

(1) washing and drying Cornus officinalis flowers;
(2) a step of preparing the dried extract of corn oil by immersing the dried corn oil in an extraction solvent and extracting the corn oil at a temperature of 5 to 100 ° C for 12 to 36 hours; And
(3) an antimicrobial agent which contains the extract of a fermented soybean oil flower produced according to the step of filtrating and concentrating the above-prepared corn oil floral extract as an active ingredient and inhibits the growth of a Staphylococcus epidermidis strain Or anti-inflammatory composition. delete delete delete delete delete delete delete delete delete

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