KR20140071738A - Cosmetic composition for alleviating dermatitis and skin irritation - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixture of natural substance extract having an activity of alleviating skin irritation and anti-inflammation as an active ingredient. The cosmetic composition for alleviating skin irritation and dermatitis, according to the present invention, contains a mixture of Sorbus commixta HEDL, Urtica thunbergiana, chinese galls knopper, and bamboo extract as an active ingredient. Since the cosmetic composition according to the present invention exhibits an excellent effect of alleviating dermatitis and skin irritation by effectively inhibiting an expression of inflammation-inducing cytokine, the cosmetic composition can be effectively used for preventing and alleviating skin trouble, improving skin redness, and preventing and alleviating dermatitis.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for alleviating skin irritation and alleviating skin irritation,

The present invention relates to a cosmetic composition comprising a natural extract mixture having skin irritation alleviation and anti-inflammatory activity as an active ingredient, and more particularly to a cosmetic composition containing a natural extract mixture having skin irritation alleviation and anti-inflammatory activity as an active ingredient, The present invention relates to a cosmetic composition having an effect of alleviating skin irritation as an active ingredient.

One of the most important functions of the skin is its protective function as a barrier. Skin, which is directly exposed to the external environment, is the most important primary line of defense against body fluids and protecting the body from harmful environments. It also acts as a barrier against toxic substances, microorganisms, physical stimuli or ultraviolet rays. However, when the skin barrier function receives various stimuli outside the skin, the skin barrier is damaged and the skin becomes dry or inflammatory reaction occurs to the skin. In addition, if the initial inflammatory reaction that occurs at this time can not be effectively controlled, it can be exacerbated by severe dermatitis.

In general, dermatitis refers to eczema dermatitis. In the acute phase, eczema (vesicle, small blister), erythema (redness of skin) and edema are seen in the acute phase, while edema and blisters are reduced in the chronic phase, while lichniscation It is a term for a skin disease in which a line of skin and palms normally present on the skin due to hardened skin becomes more pronounced), scale, and changes in skin color. Examples of such dermatitis include contact dermatitis, atopic dermatitis, and seborrheic dermatitis.

The inflammatory response in the skin begins as an action to defend against skin irritation caused by physical stimuli, chemicals, and bacteria, and various immune cells and inflammation-inducing cytokines are involved. IL-6 (interleukin-6) and IL-1β (interleukin-1β) are typical inflammation-inducing cytokines such as TNF-α. The above-mentioned TNF-α is produced by a gram-negative bacterial infection and is produced by lymphocytes activated by lipopolysaccharide (LPS), an endotoxin in the cell membrane of bacteria, and acts on white blood cells and vascular cells, IL-1β is a typical cytokine that is produced by activated mononuclear cells, epithelial cells, and vascular endothelial cells and mediates the inflammatory response. IL-6 acts on T cells and macrophages Which stimulates the immune response and is a pro-inflammatory and anti-inflammatory cytokine. Activated macrophages also activate inflammatory processes by overexpressing nitric oxide (NO) or prostaglandin E ₂ (PGE ₂ ) as well as inflammation-inducing cytokines.

Conventionally, various attempts have been made to develop cosmetics capable of imparting anti-inflammatory function to skin using natural extracts. As a typical example thereof, Korean Patent Publication No. 10-2005-0110149 (published on November 23, 2005) Korean Patent Laid-Open Publication No. 10-2006-0123886 (published Dec. 2006, 2006) discloses a skin whitening cosmetic composition containing stem and bark extract, which is obtained by sugar-hydrolyzing the stem and bark extract of Rakeboreae to increase flavonoid content Korean Patent Laid-Open Publication No. 10-2007-0001359 (published on Apr. 14, 2007) discloses a cosmetic composition having anti-inflammatory effect comprising a rhizome extract for inhibiting the production of flurostarglandine E ₂ , And the patent application No. 0761248 (registered on September 18, 2007) is an effective ingredient for extracting extracts of bamboo and golden (Scutellaria baicalensis) outermost, branches, bark, leaves, And Korean Patent Registration No. 1141802 (registered on Apr. 24, 2012) discloses a composition for the treatment of atopic dermatitis, which shows skin irritation and inflammation-relieving effects caused by acne containing cedar leaf and bamboo extract Korean Patent Publication No. 10-2001-0085371 (published on July 27, 2011) discloses a cosmetic composition containing a natural plant complex component including water lily, ginger and bamboo extract, And a cosmetic composition for improving scalp condition.

However, none of the aforementioned cosmetic compositions or compositions for treating atopy can effectively inhibit the production of inflammation-inducing cytokines synergistically by using a complex extract according to a specific combination of natural products such as the present invention, A cosmetic composition having an inflammation-reducing effect has no suggestion or suggestion at all.

The present inventors investigated the inhibition of the activity of inflammation-inducing cytokines in a wide range of extracts obtained from various natural products and combinations thereof. As a result, it was confirmed that a combination of specific natural products showed a remarkable cumulative synergistic effect, The present inventors completed the present invention by confirming the stimulation relaxation effect by repeating the test.

Accordingly, a first object of the present invention is to provide a cosmetic composition having skin stimulation alleviation and skin irritation alleviating effects by synergistically effectively inhibiting the production of inflammation-inducing cytokines by using a complex extract according to a specific combination of natural products .

The second object of the present invention is to provide a cosmetic composition which can prevent skin irritation and alleviate skin irritation by applying the base formulation of other functional cosmetic composition to prevent and improve skin irritation, improve redness, prevent and improve dermatitis .

According to a preferred aspect of the present invention for achieving the object of the present invention, it is possible to provide a skin irritation mitigation and skin irritation mitigation containing an extract mixture of Raspberry, Nettle, Rhododendron and Bamboo as an active ingredient A cosmetic composition is provided.

In the cosmetic composition according to the present invention, the extract mixture of the above-mentioned Rawan, Nettle, Rhododendron and Bamboo contains 20 to 30% by weight of the bark of bamboo, 20 to 30% by weight of the nettle leaves, 20 to 30% by weight of the bamboo, And 20 to 30% by weight of the extract.

In the cosmetic composition according to the present invention, the extract mixture of the above-mentioned Recta, Nettle, Rhododendron, and Bamboo may be an extract for an equal weight crushing mixture of the bark of the Rake, the leaves of the nettle, the dwarf, and the bamboo stalk .

In the cosmetic composition according to the present invention, it is preferable that the above-mentioned extraction mixture is contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition according to the present invention can be used in cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, pharmaceuticals, ≪ / RTI > ointment, and the like.

As described above, the cosmetic composition containing the extract mixture of Raspberry, Nettle, Opium, and Bamboo according to the present invention as an active ingredient effectively inhibits the expression of the inflammation-inducing cytokine, exhibits excellent skin irritation mitigation and skin inflammation relieving effect Therefore, it can be effectively applied to prevention and improvement of skin troubles, improvement of redness, prevention and improvement of dermatitis, and the like.

1 is a graph showing the results of Experimental Example 1 showing the degree of suppression of gene expression of inflammation-inducing cytokines in case of induction of inflammation-inducing cytokines by LPS (lipopolysaccharide) alone and in case of combination application as an extract mixture .
FIG. 2 shows the results of Experimental Example 2 in which skin irritation caused by induction of inflammation-induced cytokines by LPS is shown as mean skin response (mean score) in the case of single extract application and in the case of compound application as an extraction mixture FIG.

The cosmetic composition for alleviating skin irritation and alleviating skin irritation according to the present invention contains an extractive mixture of Rowan, Nettle, Rhododendron, and Bamboo as an effective ingredient for inhibiting the activity of inflammatory cytokines, thereby alleviating skin irritation and skin inflammation I have.

In the present invention, the bamboo is used as a raw material for the raw materials for the extraction, nettle, dwarf and bamboo in the case of the rowan, the leaf is used in the case of the nettle, and the whole is used in the case of the bamboo, And the stem is used. These raw materials are dried and dried by shade or air drying, and then used as raw materials for extraction. Preferably, the raw materials are pulverized by pulverization to improve extraction efficiency and used as raw materials for extraction.

Sorbus commixta is a deciduous broad-leaved arboreous tree distributed in Korea and Japan. It grows up to 6-8m in height. Its bark is gray-brown, there are no twigs and no hairs in winter, and the winter eye is long oval and sticky. Leaves are alternate phyllotaxis and 9 ~ 13 small leaves are lanceolate or long oval, with long pointed tip and long, pointed sawtooth at edge. There are no hairs on both sides of the small leaf, and the back side is light green. The stipule leaves early, and white flowers bloom at the end of the branch. Inflorescence has no hair, flowers are 8 ~ 10mm in diameter, and 3 styles. Spherical fruit is yellow in summer and red in autumn. In one room, the taste of the fruit is sweet and spicy, the quality is plain, the poison is not used, the taste of young branches and shells is used, the nature is cold and poisonous, the waste heat is eliminated, the cough is stopped, It is effective in the treatment of pulmonary tuberculosis, asthma, seawater, gastritis, stomach pain, vitamin A and C deficiency, and bark and fruit are used to treat paralysis.

The nettle (Urtica dioica) is a perennial plant that grows in the mountainous areas of the southern and central parts of the peninsula. The leaves and stems have hairs that contain plenty of formic acid. Leaves are opposite, petiole is long and egg-shaped. It is one male and one female in the fall, with one peduncle in the leaf axil, usually in the lower part of the peduncle, and the female flower in the upper part. The height is about 1m and the fruit is aquatic. It blooms in July and August and fruit in September and October. It has a smooth diuretic effect, which increases the amount of urine and has a good effect on the inflammatory diseases and prevention of urethra. It also acts as an adjunct to the treatment of rheumatism. Other folk remedies are also used to treat diabetes.

Rhus semialata is an insect hump made by the parasitic on the leaves of Rhus verniciflora (Rhus verniciflua) and the rhizome of Rhus verniciflora with aphids. Its appearance is irregular and irregularly divided into 2 ~ 4 pockets. The outer side is grayish grayish brown with short hairs in light brown color and is hard and brittle. The genus is empty, but grayish white color or dead worms and secretions may remain. It is used to treat chronic seawater, old cough, old diarrhea, diarrhea, cold sweat, and oil wells caused by lung collapse due to strong convergence. It is used for fecal bleeding, nosebleeds, uterine hemorrhage, trauma, premature, dermatitis and itching . The pharmacological action is known to exhibit astringent effect, antibacterial action, hepatic function-protecting action, and antioxidative action.

Bamboo or bamboo (Bamboo) is a perennial plant of rice bran and bamboo asparagus. It is also the fastest growing plant in the world for evergreen plants. The growth rate of bamboo (up to 60 cm per day) depends on the soil and climate of the area. After 4 to 5 years of budding, they all grow up. The length of the whole bamboo grows about 20m on average, and some bamboos grow up to 40m. In one room, bamboo shoots are bamboo shoots, leaves are bamboo shoots, It has the effect of eliminating wind and inflammation, lowering heat, strengthening intestines, and stimulating energy. In Byeongbok, bamboo is said to release all the readings, cleanse the whole body, release the stool, cleanse the blood, excel in prevention of aging, prevention of strokes, cold cough and fever. Hypertension, paralysis, when the eyes are tender, and neuralgia.

The extract mixture of the above-mentioned Recta, Nettle, Rhododendron and Bamboo contained in the cosmetic composition according to the present invention contains 20 to 30% by weight of the bark of birch tree, 20 to 30% by weight of the leaves of the nettle, 20 to 30% And 20 to 30% by weight of the stem, wherein the mixed weight of the bark, the nettle leaf, the obtusa, and the bamboo stem of the above-mentioned trefoil is mixed with each other and the like, May be a mixed extract.

The above bamboo leaves, nettle leaves, dwarf bamboo, and bamboo stalks are shredded or shredded, and are preferably ground and pulverized in order to increase extraction efficiency. .

In the present invention, it is preferable in the present invention to mix the four raw materials together to extract the solvent, from the viewpoint of process efficiency. However, in some cases, the same solvent and solvent amount may be used for each of the four raw materials, Next, they may be mixed in an equal amount or mixed in a weight percentage ratio as described above. The extract may be in the form of a concentrated liquid or a lyophilized powder.

In the cosmetic composition according to the present invention, the above-mentioned extraction mixture is contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition according to the present invention may be formulated into any formulation known in the art, such as a soft lotion, a nutritional lotion, a nutritional cream, a moisturizing cream, a nutritional essence, a gel, a lotion, or an ointment.

Examples of the extraction solvent which can be used for obtaining the above-mentioned extraction mixture contained as an active ingredient in the cosmetic composition according to the present invention include purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol Recycled, and butylene glycol, or a mixed solvent of two or more thereof may be used, but the present invention is not limited thereto. The addition amount of the extraction solvent is preferably 1 to 20 times the volume of the total volume of the mixed raw materials.

More specifically, the above-mentioned extraction mixture contained in the cosmetic composition of the present invention is prepared by pulverizing or crushing the bark, leaves of the nettle, leaves of the nettle, and the bamboo stalks of the leguminous leaves to form powders, immersing the above-mentioned extraction solvent for 24 to 72 hours Lt; / RTI > at room temperature. Then, the obtained extraction filtrate is filtrated and dried under reduced pressure to obtain a concentrated and mixed extract or lyophilized to obtain a powder.

The cosmetic composition according to the present invention can be formulated into, but not limited to, a basic cosmetic composition containing the above-described extraction mixture, a makeup cosmetic composition, a hair cosmetic composition, and a body cosmetic composition. For example, formulations such as soft lotion, nutritional lotion, nutritional essence, nutritional oil, moisturizing oil, nutritional cream, moisturizing cream, powder, pack, foundation, makeup base, stick, cleansing, shampoo, gel, lotion and ointment And may be used in various forms such as liquid, cream, paste, and solid, and may be formulated using conventional cosmetic preparation methods.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments, comparative examples, experimental examples, and formulation examples, but the present invention is not intended to be limited thereto.

Example 1: Preparation of Extract Mixtures of Raspberry, Nettle, Opium and Bamboo

A total of 100 g of 25 g each of the powder obtained by crushing the bark of a rowanbark, the nettle leaf, the rhizomes, and the bamboo stalks were milled with a ball mill, and the mixture was immersed in 1 L of 70 wt% ethanol aqueous solution as an extraction solvent and left at room temperature for 24 hours To obtain an extract. The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a concentrated extract mixture.

Comparative Example 1:

As a comparison, 100 g each of each of the individual raw material powders of the bark of the rowanberry, the nettle leaf, the dandelion, and the bamboo stalk were respectively taken out and placed in a separate container, immersed in 1 L of a 70% by weight aqueous ethanol solution as an extraction solvent, and left at room temperature for 24 hours To obtain an extract. The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain concentrated extracts.

Example 2:

The extract mixture obtained in Example 1 was dissolved in DMSO (dimethyl sulfoxide) as a solubilizer at a concentration of 1% by weight and used in Experimental Example 1 below.

Comparative Example 2:

Each of the extracts obtained in Comparative Example 1 was dissolved in DMSO (dimethyl sulfoxide) at a concentration of 1% by weight as a dissolving agent and used in Experimental Example 1. [

Example 3:

The extract mixture obtained in Example 1 was dissolved in a mixed solvent of purified water and butylene glycol at a weight ratio of 3: 2 at a concentration of 1% by weight and used in Experimental Example 2 and Formulations 1 to 3.

Comparative Example 3:

Each of the extracts obtained in Comparative Example 1 was dissolved in a mixed solvent of purified water and butylene glycol in a weight ratio of 3: 2 at a concentration of 1 wt%, and used in Experimental Example 2.

Experimental Example 1: Evaluation of anti-inflammatory efficacy

In order to evaluate the antiinflammatory effect of the mixed extracts, the expression level of IL-1β, IL-6, IL-8 and TNF-α, which is an inflammatory-inducing cytokine, was measured by treatment with LPS (lipopolysaccharide) . More specifically, HaCaT cells, which are keratinocytes, were first treated with LPS at a concentration of 1 μg / ml for 3 hours, and then the respective raw materials prepared in Example 2 and Comparative Example 2 were added to a final concentration of 10 μg / ≪ / RTI > for a further hour. Then, mRNA was extracted from the cells, synthesized as cDNA, real-time PCR was performed using a target primer, and the degree of gene expression of the inflammatory cytokine was finally evaluated.

As a result, when each cytokine was induced by LPS, the degree of inhibition could be evaluated for each ingredient, and the extraction mixture for the four raw materials showed the highest and remarkable induction inhibitory effect on all cytokines. Therefore, it was confirmed that the extract mixture according to the present invention is a very useful raw material for a cosmetic composition having a high anti-inflammatory effect.

The results are shown in Fig.

Experimental Example 2: Assessment of skin irritation mitigation effect

To evaluate the effect of skin irritation mitigation, 31 patients were subjected to a patch test on the arm of the arm using an IQ chamber. 0.1% by weight sodium lauryl sulfate (SLS) was used to induce skin irritation, and each extract and extract mixture prepared in Example 3 and Comparative Example 3 was applied to the skin in an amount of 1% by weight together with the same amount of SLS. The skin patch was applied for 24 hours. After removing the patch, the test area was marked with a marking pen, and skin irritation of the test area was judged at 30 minutes and 24 hours, respectively. The skin response was tested according to the method described in the following Table 1 in the CTFA (The Cosmetic, Toiletry and Fragrance Association) guidelines and the mean skin response was calculated according to the following formula 1, The degree of stimulation was judged according to the result judgment table.

Skin response Reaction grade Clinical observation 0.0 It does not show signs of inflammation; Healthy and normal skin 0.5 The appearance of the site is glossy or barely noticeable. 1.0 Some erythema 2.0 Moderate erythema, the possibility of accompanied by barely appreciable edema at the edge, the possibility of papules 3.0 Moderate erythema with total edema 4.0 It is accompanied or accompanied by erythema of intensity accompanied by edema of strength, small blisters 5.0 Reaction of intensity spreading beyond the patch area

[Equation 1]

Mean skin response (mean score)

                              (Reaction value x reaction number)

 Mean value (MS) = --------------------------------------------- - × 100

Maximum value x Total number of people x No. of visual scoring

As a result, it was confirmed that the extraction mixture of the four raw materials significantly inhibited the stimulation caused by SLS. Therefore, the extract mixture according to the present invention proved to have excellent skin irritation-reducing effect, and thus it was confirmed to be very useful as a cosmetic composition for alleviating skin irritation.

Formulation Example 1: Lotion

Lotions containing the extract mixture obtained in Example 3 were prepared in a conventional manner according to the compositional components and composition ratios shown in Table 2 below.

Composition of lotion ingredient Content (% by weight) Raspberry, nettle, rhubarb, bamboo mixed extract 3.0 glycerin 5.0 ethanol 3.0 Polyoxyethylene hardened castor oil 0.5 Polysorbate 80 0.5 Butylene glycol 3.0 Carboxyl vinyl polymer 0.5 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example 2: Nutritional essence

Nutritional essences containing the extract mixture obtained in Example 3 were prepared in a conventional manner according to the compositional components and composition ratios shown in Table 3 below.

Composition of nutritional essence ingredient Content (% by weight) Raspberry, nettle, rhubarb, bamboo mixed extract 3.0 glycerin 7.0 Butylene glycol 3.0 Glyceryl stearate / phage-100 stearate 1.0 Polysorbate 60 0.5 Sorbitan sesquioleate 0.5 Squalane 3.0 Caprylic / capric triglyceride 5.0 Shea butter 2.0 Stearyl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Arginine 0.2 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example 3: Moisturizing cream

A moisturizing cream containing the extract mixture obtained in Example 3 was prepared in a conventional manner according to the compositional components and composition ratios shown in Table 4 below.

Composition of moisturizing cream ingredient Content (% by weight) Raspberry, nettle, rhubarb, bamboo mixed extract 3.0 glycerin 7.0 Butylene glycol 5.0 Glyceryl stearate / phage-100 stearate 2.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Phitosqualan 5.0 Jojoba oil 3.0 Caprylic / capric triglyceride 10.0 Aloe butter 3.0 Wax 1.0 Stearyl alcohol 0.5 Beeryl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Xanthan gum 0.2 Arginine 0.3 Dimethicone 0.5 Beta Glucan 0.2 Preservative, fragrance Suitable amount Purified water Balance

Claims (5)

A cosmetic composition for relieving skin irritation and alleviating skin irritation, comprising an extract mixture of Raspberry, Nettle, Rhubarb, and Bamboo as an active ingredient. The method according to claim 1, wherein the extracted mixture of Raw, Nettle, Rhododendron, and Bamboo comprises 20 to 30% by weight of bark of bamboo, 20 to 30% by weight of nettle leaves, 20 to 30% by weight of bamboo, And 30% by weight of a composition for relieving skin irritation and alleviating skin irritation. The method according to claim 1, wherein the extract mixture of the above-mentioned Rectiline, Nettle, Rhododendron and Bamboo is an extract for the crushing mixture of Rectum bark, Nettle leaf, Rhododendron, A cosmetic composition for relieving inflammation. 4. The cosmetic composition for relieving skin irritation and alleviating inflammation according to any one of claims 1 to 3, wherein the extract mixture is contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 4, wherein the cosmetic composition is any formulation selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a moisturizing cream, a nutritional essence, a gel, a lotion and an ointment. Composition.

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