KR102037927B1 - Cosmetic composition composed of including extracts of drynaria fortunei and cosmetics using the same - Google Patents

Abstract

The present invention relates to a cosmetic and cosmetics having excellent antioxidant and skin stability effects, including alone or in a certain ratio, including a natural herbal ingredients, Drynaria fortunei,
More specifically, the present invention provides an extraction method that can maximize the index material of the Drynaria fortunei, and can be used as a cosmetic raw material through the antioxidant and toxicity test of the Bolchibo extract obtained through the extraction method. The present invention relates to a cosmetic composition using a bone chain beam having excellent antioxidative effect and ensuring skin stability by using the bone chain beam extract obtained alone or in a predetermined ratio. .

Description

Multi-functional cosmetic composition consisting of bone clavicle extract and cosmetics comprising the same {COSMETIC COMPOSITION COMPOSED OF INCLUDING EXTRACTS OF DRYNARIA FORTUNEI AND COSMETICS USING THE SAME}

The present invention relates to a cosmetic and cosmetics having excellent antioxidant and skin stability effects, including a single herbal ingredient (Drynaria fortunei) alone or in a certain ratio,

More specifically, by presenting the optimal formulation that can be used as a cosmetic raw material through the antioxidant, toxicity test of the Drynaria fortunei extract, using the bone chain extract obtained alone or in a certain ratio By doing so, the present invention relates to a cosmetic composition using a bone chain beam having excellent antioxidant effect and ensuring skin stability, and a cosmetic comprising the same.

Golchibori is a generic name for a generatic fern belonging to the section of Koran. Its leaf shape is similar to that of the general fern, but its root part is characterized by a lot of fine hairs. Commonly found in valleys or rocky moist areas, it refers to the root of a generous fern that grows in rock formations.

The efficacy of bone clavicle known to date is to promote bone cell function, strengthen the musculoskeletal system and prevent osteoporosis. In particular, it is effective in strengthening the musculoskeletal system by activating alkaline phosphatase and prolyl hydroxylase, and it is effective in preventing height growth and osteoporosis of children because it activates bone cells.

As the aging population grows, anti-aging products and beauty service industries are growing significantly, which are both anti-aging and safe.

In addition, along with the well-being craze, cosmetics have also been riding on this, pursuing health and beauty along with beauty, and cosmetics made up only of chemical components have moved away from consumers, and chemical cosmetics artificially synthesized to manufacture cosmetics using new materials Rather, it started looking for 'natural' materials.

Natural products are mainly used as functional cosmetic materials, and they have various functions such as whitening, moisturizing, sun protection and absorption, removal of harmful oxygen, promoting collagen synthesis, anti-wrinkle immunity and anti-inflammatory, hair growth agents, and so on.

In addition, with the development of cosmetics-related technologies, new natural products are continuously being developed.

Republic of Korea Patent Registration 10-0539298 (Registration Date 2005.12.21) Republic of Korea Patent Registration 10-1315440 (Registration date 2013.09.30) Republic of Korea Patent Registration 10-0515419 (Registration date 2005.09.09) Republic of Korea Patent Registration 10-0530880 (Registration date 2005.11.17) Republic of Korea Patent Registration 10-0858449 (Registration date 2008.09.08) Republic of Korea Patent Registration 10-1483440 (Registration date 2015.01.12) Republic of Korea Patent Registration 10-1809545 (Registration Date 2017.12.11)

The present invention proposes an optimal formulation that can be used as a cosmetic ingredient through the antioxidant and toxicity test of the extract Dolchinaria (Drynaria fortunei), the antioxidant and skin stability including the Dynaria fortunei extract alone or in part It is an object of the present invention to provide a cosmetic composition using an excellent bone clasp and a cosmetic comprising the same.

In order to achieve the above object,

The present invention provides a multi-functional cosmetic composition using a bone clabo extract, which comprises the bone extract as a whole amount or 1.0 ~ 99.0 wt% of the total weight of the cosmetic composition.

Another embodiment provides a multi-functional cosmetic composition using a bone chain beam extract 1.0 ~ 99.0 wt%, and a bone extract extract is composed of a mixture of natural antioxidant extract 1.0 ~ 99.0 wt%.

As another embodiment provides a multi-functional cosmetic composition using a bone chain beam extract 1.0 ~ 99.0 wt%, and a natural skin stabilizing, moisturizing extract 1.0 ~ 99.0 wt% mixture.

And it provides a cosmetic comprising a golbobo extract consisting of the above-described multifunctional cosmetic composition 1.0 to 50.0 wt% of the total weight of the cosmetic.

Multi-functional cosmetic composition and a cosmetic containing the same, consisting of a bone clabo extract according to the present invention has the following effects.

first. Provided is a cosmetic and cosmetics with excellent antioxidant and skin stability effect, including alone or in a certain ratio.

second. By using Drynaria fortunei and natural antioxidant extract together to form a multi-functional cosmetic composition has a high antioxidant properties.

third. The combination of Drynaria fortunei and natural skin stabilizer and moisturizing extract have high skin stability and moisturizing effect.

fourth. By presenting a formulation having excellent skin absorption rate and formulation stability using the Bolchibo extract, it provides a multifunctional cosmetic using Bolchibo extract.

1 is a graph showing the absorbance of each dilution concentration of the SJ-DEF treatment group.
Figure 2 is a graph showing the absorbance of each dilution concentration of the positive control group (SDS treatment group).
Figure 3 is a graph showing the cell survival rate by dilution concentration of the SJ-DEF treatment group.
Figure 4 is a graph showing the cell survival rate by dilution concentration of the positive control group (SDS treatment group).
Figure 5 is a graph showing the absorbance of each dilution concentration of the SJ-DEF treatment group.
Figure 6 is a graph showing the absorbance according to dilution concentration of Ascorbic acid treatment group.
Figure 7 is a graph showing the antioxidant capacity according to the dilution concentration of the SJ-DEF treatment group.
Figure 8 is a graph showing the antioxidant capacity according to the diluted concentration of Ascorbic acid treatment group.
9 is a graph showing the elastase inhibitory activity of Oleanolic acid.
10 is a graph showing the elastase inhibitory activity of the SJ-DEF sample.
11 is a graph showing the degree of skin sebum reduction.
12 is a graph showing the rate of skin sebum improvement.
13 is a graph showing the trend of skin moisture content improvement.
14 is a graph showing the skin moisture content improvement rate.

The present invention is a multi-functional cosmetic composition and a cosmetic comprising the bone clavicle extract made through a test analysis of the skin toxicity, safety and storage safety of bone clavicle extract, and the selection of the optimal formulation using the optimal bone clavicle extract It is about.

Hereinafter, look at the specific technical configuration of the multi-functional cosmetic composition and cosmetics comprising the same as the bone clavicle extract according to the present invention.

First, let's take a look at the indicators of bone breakers.

The indicator substance refers to a substance that is a standard for identifying herbal medicines, plants, etc., and is not included in other raw materials or refers to a substance in which a specific substance is detected and characterized.

According to researches and papers conducted to date, hesperidin and Naringin are widely extracted from the bone clasp (Table 1).

Characteristics of Bone Clavicle Extract Item
Target raw material Bone Clavicle Extract main ingredient Hesperidin
Naringin main ingredient
Chemical formula

line Flavonoid effect Antioxidant, Wrinkle Improvement

Therefore, in the present invention, by setting the two substances as the main target to extract and purify bone extracts extracts and extracts a substance that can exhibit the activity as a cosmetic, it is an indicator material.

The bone chain beam extraction method is divided into hot water extraction process and ethanol extraction process.

In the past, bone crab, which is a herbal ingredient, was mainly used in foods in the form of dried state or extract. There has been no test analysis to apply such a bone clasp to cosmetics.

The hot water extract of Golchibo is added to Golchibo and distilled water to 1 to 10 by weight ratio, Golchibo and distilled water is added in a ratio of 1 to 10, extracted for 3 hours at 80 ~ 95 ℃, the extracted solution is filtered through a filter paper, and the filtrate is rotated Concentrated under reduced pressure concentrator, the concentrate was freeze-dried with a lyophilizer and ground to a powder prepared.

The ethanol extract of the golbobo is added to the golbobo 70% (v / v) ethanol, golbobo and 70% ethanol in a 1:10 weight ratio, and extracted for 12 hours at 25 ~ 35 ℃, the same extraction The process was repeated three times, and the extracted solution was filtered through a filter paper, the filtrate was concentrated with a rotary vacuum concentrator, the concentrate was lyophilized with a freeze dryer, and then pulverized to prepare a powder.

The multifunctional cosmetic composition according to the present invention, as an example, comprises the bone extract as a whole, or comprises 1.0 to 99.0 wt% of the total weight of the cosmetic composition.

In another embodiment, the multi-functional cosmetic composition is a mixture of 1.0 ~ 99.0 wt% of the bone chain beam extract, 1.0 ~ 99.0 wt% of natural antioxidant extract.

At this time, the natural antioxidant extract,

The vortex is added to distilled water, and the vortex and the distilled water are added in a weight ratio of 1:15 to 30, extracted with cooling under reflux at 65 to 80 ° C. for 4 to 7 hours, and the extracted solution is filtered with filter paper (Whatman No. 2). After filtering, the filtrate was concentrated to a temperature of 55 ~ 65 ℃ using a rotary decompression concentrator 10 ~ 30 wt% obtained by extracting;

To dry spirit (Smilacis glabrae Rhizoma, SG) is added to the distilled water, the bokbokyeong and distilled water is added so as to make a weight ratio of 1: 5 to 10, extracted with reflux cooling at 65 ~ 80 ℃ for 4 to 7 hours, The extracted solution was filtered through a filter paper (Whatman No. 2), and then the filtrate was concentrated using a rotary pressure reducer at a temperature of 55 to 65 ° C., 10 to 30 wt%;

Green tea is added to the distilled water at 65 ~ 75 ℃, the green tea and distilled water is added so as to make a weight ratio of 1: 15 to 25, 10 ~ 30 wt% green tea extract obtained by extracting for 10 to 30 minutes in a 70 ℃ water bath;

Rice bran powder is added to distilled water, and the rice bran powder and distilled water are added in a weight ratio of 1:20 to 30, heated to 100 ° C., extracted for 3 to 5 hours, and the extracted solution is filtered with filter paper (Whatman No. 2). Then, 10 ~ 30 wt% of rice bran extract obtained by concentrating the filtrate at a temperature of 55 ~ 65 ℃ using a rotary pressure reducer;

Grape seed powder was added to 70% (v / v) methanol, and the grape seed powder and methanol were added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution was filtered through a filter paper (Whatman No. 2), the filtrate was concentrated by using a rotary decompression concentrator at a temperature of 55 ~ 65 ℃ 10 ~ 30 wt%;

Flaxseed powder was added to 70% (v / v) methanol, and the flaxseed powder and methanol were added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution was filtered through a filter paper (Whatman No. 2), and then the filtrate was concentrated to a temperature of 55 ~ 65 ℃ using a rotary pressure reducer 10 ~ 30 wt%;

Pomegranate seed powder is added to 70% (v / v) methanol, and the pomegranate seed powder and methanol are added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution is filtered through After filtering to (Whatman No. 2), the filtrate was concentrated by using a rotary pressure reducer at a temperature of 55 ~ 65 ° C 10 ~ 30 wt%;

Combination examples of the natural antioxidant extract are shown in Table 2 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Wasong Extract 25 wt% 15 wt% 20 wt% 25 wt% 30 wt% Tobokyeong Extract 25 wt% 20 wt% 25 wt% 20 wt% 10 wt% Green tea extract 10 wt% 15 wt% 10 wt% 10 wt% 10 wt% Rice bran extract 10 wt% 15 wt% 10 wt% 10 wt% 10 wt% Grape seed extract 10 wt% 10 wt% 15 wt% 15 wt% 15 wt% Flaxseed extract 10 wt% 15 wt% 10 wt% 10 wt% 10 wt% Pomegranate Seed Extract 10 wt% 10 wt% 10 wt% 10 wt% 15 wt% Sum 100 wt% 100 wt% 100 wt% 100 wt% 100 wt%

Orostachys japonicus A. Berger, which constitutes the natural antioxidant extract, is a perennial herbaceous plant of Crassulaceae, which is called lymeum, oxsong, leaf herb, etc., for a long time hepatitis, pneumonia, hemostasis, eczema, burns, edema and cancer. It has been used as a cure. The main components of the turmeric include triterpenoids such as friedelin, epi-friedlanol, glutinone and glutinol, sterol-based substances such as β-sitosterol and campesterol, fatty acid esters and flavonoids such as kaempferol and quercetin, 4-hydroxybenzoic acid, 3, 4-dihydroxybenzoic acid and gallic acid aromatic acid

The Sbok is a herb that refers to the root diameter of the blue vines of Smilacis glabrae Rhizoma (SG). Tobok-ryeong is found in the herbaceous tree and has been used as an antidote for the treatment of chronic skin diseases, gonorrhea, diuresis, syphilitic skin diseases. The main components of Tobok-ryeong are saponins such as parillin and smilacin, and they contain glycosides, ophiopogonin and mucus, which are separated from the roots, and are known to play an important role in promoting the body's immunity and protecting organs from various bacterial infections. have.

The main components of the green tea is catechin, caffeine, protein, free amino acid, starch, fiber, pectin and chlorophyll, flavonol derivatives, plant pigments such as anthocyanin and resins, essential oils, vitamins, minerals, etc. Insoluble is 60-65%. Compared to other plants, it contains catechin and caffeine as well as amino acids, potassium, fluorine, zinc, manganese, vitamin C, vitamin E, and chlorophyll. Tea contains body-regulating catechins, caffeine, polysaccharides, antioxidant vitamins, saponins, minerals, and GABA (-aminobutyric acid).

The rice bran contains a large amount of lysine, an essential amino acid lacking in grains, and at least 70% of the constituent fatty acids are composed of unsaturated fatty acids such as oleic acid, linoleic acid and linolenic acid. Among these components, linoleic acid and linolenic acid are omega-6 and omega-3 polyunsaturated fatty acids (PUFA), respectively, belonging to essential fatty acids that cannot be synthesized in the body.

Grapes (Vitis vinifera Linne) are rich in organic acids, vitamins and minerals, as well as phenolic compounds such as anthocyanins, benzoic acids, flavonol procyanidin and prophylgallate, as well as resveratrol, known as an antioxidant, which lowers cholesterol levels in the blood It is effective in preventing and treating stroke and heart disease. It has anticancer, antioxidant and antifungal effects. In particular, grape seeds contain a large amount of vitamin E, a representative antioxidant, and tocotrienol in vitamin E has functions such as antioxidant activity and anticancer aging delay.

Flaxseed acts as an anticancer agent to clear blood and lower blood sugar. It is rich in omega-3s, helping to improve various vascular diseases, lowering cholesterol levels and increasing immune function. Flaxseed contains 28% fiber. Dietary fiber aids in bowel movements, facilitates toxin excretion, and improves troubles and atobi, which also helps skin care.

Pomegranate contains piperidine alkaloids, isopelletierine (primary), pseudopelletierine, methyl pelletierine, and methyl isopelletierine, which are 0.6%, 0.5% of stem bark, and 0.4% of bark. Pomegranate rind contains tannin (terchebin, granatin B. punicacortein A-D, casuarinin, 2-O-galloypunicalin), sugars, resins, mannitol, inulin, succinic acid, oxalic acid, pectin, Ca, isoquercetin, and β on the leaves -Cytosterol, mannitol, seeds contain punic acid, bark contains manitol, sorbitol.

As another embodiment, the multi-functional cosmetic composition is composed of a mixture of 1.0 ~ 99.0 wt% of bone chain beam extract, 1.0 ~ 99.0 wt% of natural skin stability, moisturizing extract,

The natural skin stability, moisturizing extract,

Ogapi was added to distilled water, and the agapi and distilled water were added in a weight ratio of 1:15 to 25, and extracted at 30 to 40 ° C. for 5 to 10 hours, and the extracted solution was filtered with filter paper (Whatman No. 2), 10-30 wt% of the extract of Ogapi obtained by concentrating the filtrate at a temperature of 55-65 ° C. using a rotary pressure reducer;

Dry sulfur white is added to 90% (v / v) ethanol, and the sulfur white and ethanol are added in a 1:10 to 15 weight ratio to extract for 5 to 10 hours at 35 ~ 65 ℃, the extracted solution is filter paper (Whatman No .2) 10 to 30 wt% of a yellow white extract obtained by concentrating the filtrate using a rotary pressure reducer at a temperature of 55-65 ° C .;

Dry Angelica was added to 90% (v / v) ethanol, and the Angelica and ethanol 1: 1 to 10 to 15 by weight ratio was added to extract for 15 to 25 hours at 55 ~ 65 ℃, the extracted solution was filter paper (Whatman No .2), and then the filtrate was concentrated by using a rotary pressure reducer at a temperature of 55 ~ 65 ℃ 10 ~ 30 wt%;

Allantoin is added to 90% (v / v) ethanol, and the allantoin and ethanol are added in a 1:10 to 15 weight ratio, and extracted at 55 to 65 ° C. for 15 to 25 hours, and the extracted solution is filtered by Whatman No. 2), after the filtrate was concentrated using a rotary pressure reducer at a temperature of 55 ~ 65 ℃ allantoin extract 10 ~ 30 wt%;

Add Schizandra chinensis to distilled water, add Schizandra distilled water to a weight ratio of 1: 8 to 15, and extract the mixture by heating to 100 ° C. for 2 to 4 hours using a reflux heater, and extracting the supernatant by centrifugation. After taking, 10 ~ 30 wt% of Schisandra chinensis extract obtained by concentrating the filtrate filtered with filter paper (Whatman No. 2) at a temperature of 55 ~ 65 ℃ using a rotary pressure reducer;

Add lotus leaf, lotus stem, and lotus root in the same weight, and add the powder to 80% (v / v) ethanol, but add the lead powder and ethanol in a ratio of 1: 8 to 12, and then add 4 at 65 ~ 80 ℃. Extracted while cooling under reflux for ˜ 7 hours, the extracted solution was filtered through a filter paper (Whatman No. 2), and then the filtrate was concentrated by using a rotary pressure reducer at a temperature of 55 ~ 65 ℃ 10 extract ˜30 wt%;

Formulation examples of the natural skin stabilizer and moisturizing extracts are shown in Table 3 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Ogapi Extract 20 wt% 15 wt% 10 wt% 25 wt% 30 wt% Yellow White Extract 20 wt% 25 wt% 15 wt% 15 wt% 25 wt% Angelica Extract 20 wt% 20 wt% 25 wt% 15 wt% 10 wt% Allantoin extract 20 wt% 20 wt% 30 wt% 25 wt% 15 wt% Schizandra Extract 10 wt% 10 wt% 10 wt% 10 wt% 10 wt% Lotus extract 10 wt% 10 wt% 10 wt% 10 wt% 10 wt% Sum 100 wt% 100 wt% 100 wt% 100 wt% 100 wt%

Acanthhopanacis cortex (Aantthopanax sessiliflorum Seeman (Araliaceae)) has been used as a medicinal herb as a medicinal herb with no toxicity and no side effects. For the purpose of using the roots and bark of Ogapi.

The baekbaek (黃柏, Phellodendri cortex) is a stem bark peeled off the main bark of the Phellodendron amurense Ruprecht belonging to the rutaceae plants. Yellow and white alkaloids contain various kinds of alkaloids. The main components are obacunone, obaculactone, berberine and mucus. Among the components of baekbaek, berberine is known to have a blood pressure lowering action, antioxidant action, blood coagulation promoting action, antibacterial action, antipyretic action and anti-inflammatory action.

Angelica gigantis Radix is a perennial belonging to the genus Umbeliferae and is a representative blood pill along with succinate, glue, peony. The Angelica gigas is the root of Angelica gigas Nakai (mountain and Umbeliferae).

The taste of Angelica Angelica is slightly bitter and has an unusual aroma. Young leaves are used for edible and ornamental purposes, and because of the blood replenishment effect in oriental medicine, it is known that the roots are used to treat gynecological diseases caused by anemia or blood circulation disorder. The main pharmacological ingredient is decursin, a coumarin derivative, and decursinol, umbelliferon, and β-sitosterol.

The allantoin is a natural compound produced by a variety of organisms, including animals, bacteria and plants, such as perennial grasses of comfrey root, oak, wheat germ, tobacco seeds and urine. The pharmacological action of allantoin mainly activates the cell activity directly and indirectly. Along with the pharmacological action that promotes metabolism, there are no cases of allergies or side effects, and it has higher safety than other chemicals by effectively protecting the skin.

The Schizandra chinensis Baillon is a deciduous woody plant belonging to the Magnoliaceae, and is called the herbal medicine Schizandra (五味子). Common ingredients of Schisandra chinensis consist of 80% moisture, 1.0% fat, 1.2% protein, and 14% total sugar. Fruit juice contains fructose, sucrose, glucose and maltose. It contains sugars and various organic acids, as well as vitamins A and C.

The lotus is one of the traditional Korean ingredients, and its origin is southern Asia and northern Australia, but is widely distributed in Southeast Asia such as China, India, Korea, and Japan, and is a perennial grass growing in ponds and wetlands. Common ingredients of lotus leaf are 63.8g of carbohydrate, 16.9g of protein, 1g of fat and 9.3g of ash per 100g. Calcium content is 20 times higher than green tea per 100g dry sample, vitamin A is the highest as 16.9mg. And lotus root has higher content of vitamin C and potassium than other root plants, and tastes sweet and warm. The effects of lotus root hemostasis and diarrhea and vomiting, releasing the blood, and if you drink the fresh life has the effect of loosening the poison.

Cosmetics comprising the bone chain beam extract according to the present invention consists of the bone chain beam hot water extract alone, or the cosmetic composition comprising a natural antioxidant extract, natural skin stability, moisturizing extract to 1.0 ~ 50.0 wt% of the total weight of the cosmetic It is made to include.

More specifically,

Cosmetic composition 1.0 to 50.0 wt%;

Water 85.0 ~ 92.0 wt%, Disodium EDTA 0.01 ~ 0.05 wt%, Glycerin 2.5 ~ 8.5 wt%, Butylene Glycol 1.5 ~ 7.5 wt%, 10.0 sodium hyaluronate 1% Solution 49.0-90.0 wt% of 100.0 wt% of the first composition composed of 0.2-3.5 wt% of a mixture;

Alcohol 73.0 ~ 79.0 wt%, 1,2-hexanediol 15.0 ~ 23.0 wt%, PIG-40 Hydrogenated Castor Oil 1.5 ~ 6.5 wt %, 100.0 wt% of the second composition 1.0-15.0 wt%;

Cosmetic composition 1.0 to 50.0 wt%,

Water 55 ~ 65 wt%, Disodium EDTA 0.01 ~ 0.1 wt%, Dipotassium Glycyrrhizate 0.05 ~ 0.2 wt%, Butylene Glycol 20 to 35 wt%, Glycerin 2.5 to 12.5 wt%, Allantoin 0.05 to 0.5 wt%, 1% Sodium Hyaluronate 1% Solution 1.0 to 8.5 wt%, Beta-Glucan -Glucan) 35.0 to 65.0 wt% of the third composition, which is composed of a mixture of 0.01 to 0.08 wt%, 1,2-hexane diol (1,2-Hexanediol) 1.5 to 7.5 wt%,

2.0 to 15.0 wt% of a fourth composition formed by mixing 98.0 to 99.8 wt% of purified water and 0.2 to 2.0 wt% of xanthan gum;

10.0 to 30.0 wt% of the fifth composition formed by mixing 98.0 to 99.8 wt% of purified water and 0.2 to 2.0 wt% of carbomer,

2.0 to 15.0 wt% of the sixth composition formed by mixing 95.0 to 99.0 wt% of purified water and 1.0 to 5.0 wt% of triethanolamine,

Composition 7 composed of a mixture of PIG-60 Hydrogenated Castor Oil 2.0 to 9.5 wt%, Alcohol 89.0 to 97.0 wt%, Fragrance 0.2 to 1.5 wt% The essence is formulated with a mixture of 0.5-10.5 wt%.

Cosmetic composition 1.0 to 50.0 wt%,

Water 42.0 ~ 48.0 wt%, Disodium EDTA 0.01 ~ 0.08 wt%, Fiji / Fiji-17 / 6 Copolymer (PEG / PPG-17 / 6 Copolymer) 0.05 ~ 0.5 wt %, Glycerin 29.0 to 37.0 wt%, DL-Panthenol 3.5 to 12.0 wt%, Allantoin 0.05 to 0.5 wt%, Biosaccharide Gum-1 2.0 to 20.0 to 55.0 wt% of an eighth composition formed by mixing 10.5 wt%;

0.1 to 5.0 wt% of a ninth composition formed by mixing 90.0 to 97.0 wt% of butylene glycol and 3.0 to 10.0 wt% of xanthan gum;

10.0 to 30.0 wt% of the 10th composition formed by mixing 96.5 to 99.8 wt% of purified water and 0.2 to 3.5 wt% of hydroxyethylcellulose,

10.0 to 30.0 wt% of the 11th composition formed by mixing 96.5 to 99.8 wt% of purified water and 0.2 to 3.5 wt% of carbomer,

3.0 to 20.0 wt% of composition 12, which is formed by mixing 96.5 to 99.5 wt% of purified water and 0.5 to 3.5 wt% of triethanolamine,

PIG-60 Hydrogenated Castor Oil 2.5 to 7.5 wt%, 1,2-Hexanediol 23.0 to 30.0 wt%, Alcohol 62.0 to 74.0 wt %, Fragrance (Fragrance) A mask sheet is formed by mixing 1.5-15.5 wt% of the 13th composition, which is composed of a mixture of 0.2-2.5 wt%.

And,

Cosmetic composition 1.0 to 50.0 wt%,

Cetyl PEG / PPG-10 / 1 Dimethicone 22.0 ~ 35.0 wt%, Cyclopentasiloxane 42.0 ~ 48.0 wt%, Dimethicone 23.0 ~ 30.0 wt 3.0 to 20.0 wt% of the 14th composition which is formed by the mixing of%,

Water 85.0 ~ 93.0 wt%, Glycerin 2.5 ~ 8.5 wt%, Dipropylene Glycol 1.5 ~ 6.5 wt%, Sodium Chloride 0.2 ~ 2.5 wt% 45.0 to 95.0 wt% of the 15th composition to be formed;

A waterdrop cream is formed by mixing 0.1-7.5 wt% of the 16th composition, which is composed of 95.0-99.0 wt% of 1,2-hexanediol and 1.0-5.0 wt% of fragrance. .

Let us look through the specific examples of the optimal formulation using the cosmetic composition.

Specific formulations of the skin comprising the bone clavicle hydrothermal extract according to the present invention are as follows.

A.

Purified Water 79.53 g

0.02 g of disodium EDTA

Glycerin 5.00 g

Butylene Glycol 4.00 g

1.00 g of 1% Sodium Hyaluronate

B.

Alcohol (8.00 g)

2.00 g of 1,2-hexanediol (1,2-Hexanediol)

0.40 g of PEG-40 Hydrogenated Castor Oil

Fragrance 0.05 g

C.

Golbobo Hot Water Extract 20.00 g

Specific formulation of the essence comprising a bone chain hydrothermal extract according to the present invention is as follows.

A.

Purified water 32.32 g

0.02 g of disodium EDTA

0.05 g Dipotassium Glycyrrhizate

Butylene Glycol 14.00 g

Glycerin 4.00 g

Allantoin 0.05 g

2.00 g Sodium Hyaluronate 1% Solution

0.01 g of beta-glucan

2.00 g of 1,2-hexanediol (1,2-Hexanediol)

B.

9.90 g of purified water

Xanthan Gum 0.10 g

C.

Purified water 19.80 g

Carbomer 0.20 g

D.

Purified Water 10.00 g

0.20 g of Triethanolamine

E.

0.30 g of PIG-60 Hydrogenated Castor Oil

Alcohol (5.00 g)

Fragrance 0.05 g

F.

Golbobo Hot Water Extract 20.00 g

Specific formulation of the mask sheet comprising a bone chain hydrothermal extract according to the present invention is as follows.

A.

20.75 g of purified water

0.02 g of disodium EDTA

0.05 g of PIG / PPG-17 / 6 copolymer (PEG / PPG-17 / 6 Copolymer)

Glycerin 14.00 g

DL-Panthenol 4.00 g

Allantoin 0.05 g

2.00 g of Biosaccharide Gum-1

B.

Butylene Glycol 1.50 g

Xanthan Gum 0.08 g

C.

Purified water 19.80 g

0.20 g of hydroxyethylcellulose

D.

Purified water 19.80 g

Carbomer 0.20 g

E.

10.80 g of purified water

0.20 g of Triethanolamine

F.

0.30 g of PIG-60 Hydrogenated Castor Oil

2.00 g of 1,2-hexanediol (1,2-Hexanediol)

Alcohol (5.00 g)

Fragrance 0.05 g

G.

Golbobo Hot Water Extract 20.00 g

Specific formulation of the water drop cream comprising a bone chain hydrothermal extract according to the present invention is as follows.

A.

3.00 g of cetyl PEG / PPG-10 / 1 Dimethicone

5.00 g of Cyclolopentasiloxane

Dimethicone 3.00 g

B.

Purified Water 78.15 g

Glycerin 5.00 g

3.00 g of dipropylene glycol

Sodium Chloride 0.80 g

C.

2.00 g of 1,2-hexanediol (1,2-Hexanediol)

Fragrance 0.05 g

D.

Golbobo Hot Water Extract 20.00 g

To achieve the same skin formulation as in Example 1,

In place of 20.00 g of bone clavicle hydrothermal extract,

The difference is that a mixture of 10.00 g of bone chain hydrothermal extract and 10.00 g of natural antioxidant extract is used.

At this time, the natural antioxidant extract is composed of a mixture of Waksong extract 2.50 g, Tobokyeong extract 2.50 g, green tea extract 1.00 g, rice bran extract 1.00 g, grape seed extract 1.00 g, flaxseed extract 1.00 g, pomegranate seed extract 1.00 g.

To form the same essence formulation as in Example 2,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of Bolbobo hot water extract and 10.00 g of natural antioxidant extract.

At this time, the natural antioxidant extract is composed of a mixture of Waksong extract 2.50 g, Tobokyeong extract 2.50 g, green tea extract 1.00 g, rice bran extract 1.00 g, grape seed extract 1.00 g, flaxseed extract 1.00 g, pomegranate seed extract 1.00 g.

To form the same mask sheet formulation as in Example 3,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of Bolbobo hot water extract and 10.00 g of natural antioxidant extract.

At this time, the natural antioxidant extract is composed of a mixture of Waksong extract 2.50 g, Tobokyeong extract 2.50 g, green tea extract 1.00 g, rice bran extract 1.00 g, grape seed extract 1.00 g, flaxseed extract 1.00 g, pomegranate seed extract 1.00 g.

In the same water-drop cream formulation as in Example 4,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of Bolbobo hot water extract and 10.00 g of natural antioxidant extract.

At this time, the natural antioxidant extract is composed of a mixture of Waksong extract 2.50 g, Tobokyeong extract 2.50 g, green tea extract 1.00 g, rice bran extract 1.00 g, grape seed extract 1.00 g, flaxseed extract 1.00 g, pomegranate seed extract 1.00 g.

To achieve the same skin formulation as in Example 1,

In place of 20.00 g of bone clavicle hydrothermal extract,

The difference is that a mixture of 10.00 g of bone chain hydrothermal extract and 10.00 g of natural skin stabilizer and moisturizing extract is used.

At this time, the natural skin stabilization, moisturizing extract is composed of a mixture of Ogapi extract 20.00 g, yellow white extract 20.00 g, Angelica extract 20.00 g, allantoin extract 20.00 g, Schisandra chinensis extract 10.00 g, lotus extract 10.00 g.

To form the same essence formulation as in Example 2,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of bone chain supplement hot water extract and 10.00 g of natural skin stabilizer and moisturizing extract.

At this time, the natural skin stabilization, moisturizing extract is composed of a mixture of Ogapi extract 20.00 g, yellow white extract 20.00 g, Angelica extract 20.00 g, allantoin extract 20.00 g, Schisandra chinensis extract 10.00 g, lotus extract 10.00 g.

To form the same mask sheet formulation as in Example 3,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of bone chain supplement hot water extract and 10.00 g of natural skin stabilizer and moisturizing extract.

At this time, the natural skin stabilization, moisturizing extract is composed of a mixture of Ogapi extract 20.00 g, yellow white extract 20.00 g, Angelica extract 20.00 g, allantoin extract 20.00 g, Schisandra chinensis extract 10.00 g, lotus extract 10.00 g.

In the same water-drop cream formulation as in Example 4,

In place of 20.00 g of bone clavicle hydrothermal extract,

There is a difference in using a mixture of 10.00 g of bone chain supplement hot water extract and 10.00 g of natural skin stabilizer and moisturizing extract.

At this time, the natural skin stabilization, moisturizing extract is composed of a mixture of Ogapi extract 20.00 g, yellow white extract 20.00 g, Angelica extract 20.00 g, allantoin extract 20.00 g, Schisandra chinensis extract 10.00 g, lotus extract 10.00 g.

Hereinafter, look at the experimental results according to the present invention.

<Experimental Results>

Key performance indicator

unit
result Objective measuring method sample water
(n≥5)
Test specification 1. Extract yield evaluation % ≥ 10% 20 Certified Test Report 2. Cytotoxicity Assessment % 100 ± 10% 5 Official test report 3. Antioxidant efficacy evaluation % ≥ 80% 5 Official test report 4. Evaluation of wrinkle improvement efficacy % ≥ 40% 5 Official test report 5. Evaluation of stability of raw materials day 30 days 5 Official test report 6. Oily water measurement evaluation % 120% 5 Official test report 7. Patch test Voice / positive voice 5 Official test report

1. Evaluation Indicator 1: Export Yield Evaluation

The details of the hot water extraction method and the ethanol extraction method for evaluating the yield of hot water extract of bone chain beam are as shown in Tables 5 and 6 below.

Hydrothermal Extraction Method Extraction process 1.10 times distilled water
2. Heat 1 to 80 ~ 95 ℃ for 3 hours
3. Filter with filter paper and concentrate with rotary depressurizer.
4. The extract was dried with lyophilizer 3 to prepare a powder. Extraction Example time Temperature division

3 hours 60 ℃ Sample 1 80 ℃ Sample 2 100 ℃ Sample 3
4 hours
60 ℃ Sample 4 80 ℃ Sample 5 100 ℃ Sample 6
5 hours
60 ℃ Sample 7 80 ℃ Sample 8 100 ℃ Sample 9 6 hours 30 ℃ Sample 10

Ethanol Extraction Method Extraction process 1.Add 70% ethanol of sample and 10 times of sample
2. Heat 1 to 25 ~ 35 ℃ for 12 hours
3. Proceed 2 3 times
4. Filter with filter paper and concentrate with rotary depressurizer.
5. The extract of 4 was dried with a lyophilizer to prepare a powder. Extraction Example time Temperature division

12 hours Room temperature Sample 1 40 ℃ Sample 2 50 ℃ Sample 3 60 ℃ Sample 4
24 hours
Room temperature Sample 5 40 ℃ Sample 6 50 ℃ Sample 7 60 ℃ Sample 8

 The average yield of hot water extracts of hot bone extracts through hot water extraction and ethanol extraction process for bone clavicle is shown in Table 7 below.

Average Yield Using Hot Water Extraction and Ethanol Extraction Extraction method row data yield Hydrothermal extraction 18% Ethanol extraction 20%

2. Evaluation index 2: evaluation of cytotoxicity

1) Cell line selection and cell culture

HaCaT cells were used to assess the cell viability of the samples. Cells were seeded at 1.5 × 10 ⁶ cells / dish in a 100 mm ² culture dish, followed by Dulbecco's Modified Eagle's containing penicillin (100 IU / mL), streptomycin (100 μg / mL) and 10% Fetal bovine serum (FBS). Medium (DMEM) medium was added and cultured in an incubator (Sanyo, Japan) containing 37 ° C. and 5% carbon dioxide. Cells were passaged every 2 days and used in this experiment.

2) Cell viability test using MTT (Thiazolyl Blue Tetrazolium Bromide) assay

The MTT assay is a test to measure the ability of mitochondria to reduce MTT tetrazolium, a yellowish water-soluble substrate, to formazan with a reddish purple color by the dehydrogenase action of cells. Reduction of MTT occurs only in cells with active metabolism and is widely used to evaluate cell viability of samples. In this experiment, subcultured HaCaT cells were dispensed in a 96-well plate at 1.5 × 10 ⁴ cells / well, incubated for 24 hours in an incubator containing 37 ° C and 5% carbon dioxide, and the culture media replaced with serum free media. It was. Subsequently, the bone chain hydrothermal extract (hereinafter referred to as 'SJ-DEF') was treated with the cells so that the final treatment concentration was 1 μg / mL, 10 μg / mL, 100 μg / mL, and 1000 μg / mL. (SDS; Sodium dodecyl sulfate) was incubated in the cell culture conditions for 24 hours by intracellular treatment of SDS so that the final treatment concentration is 1 μg / mL, 10 μg / mL, 100 μg / mL and 1000 μg / mL. After incubation for 24 hours, 20 uL of MTT solution (5 mg / mL) was applied to each well in a shaded state, and reacted for 2 hours. Then, the supernatant was removed, and the resulting formazan was completely dissolved in 150 μl of DMSO. Absorbance at 540 nm was measured with Biotek Synergy-HT, USA).

The test was repeated three times, and the result was expressed as the cell survival rate compared to the untreated group by the following equation and expressed as the mean ± standard deviation of the three experiments.

3) results

MTT assay of samples SJ-DFE 1 μg / mL, 10 μg / mL, 100 μg / mL and 1000 μg / mL and positive control SDS 1 μg / mL, 10 μg / mL, 100 μg / mL and 1000 μg / mL The test results of absorbance and cell viability are shown in Tables 8 to 11 below, and the results are graphically represented as shown in FIGS. 1 to 4.

The SJ-DFE treated group showed 91.67% at 1 μg / mL, 83.28% at 10 μg / mL, 75.99% at 100 μg / mL, and 45.13% at 1000 μg / mL, respectively. The treatment concentration showed a statistically significant difference (p <0.05) compared with the untreated group.

The SDS-treated group used as a positive control group showed 88.48% at 1 μg / mL, 18.97% at 10 μg / mL, 4.39% at 100 μg / mL, and 4.23% at 1000 μg / mL, respectively. All treatment concentrations showed a statistically significant difference (p <0.05) compared to the untreated group.

Statistical analysis result of SJ-DEF treatment group (comparison after concentration treatment) X ² Degrees of freedom Significance density 41.960 4 <0.001

Probability p (kruskal-Wallis test)

Absorbance and Cell Viability of SJ-DEF Treated Group (Mean ± Standard Deviation) Sample concentration (㎍ / mL)

0
(Untreated group ^a )
One
10
100
1000 Absorbance (OD 540) 1.208 ± 0.017 1.107 ± 0.028 1.006 ± 0.032 0.918 ± 0.041 0.545 ± 0.024 p-value ^b - <0.001 * <0.001 * <0.001 * <0.001 * Cell survival rate (%) 100.00 ± 0.00
91.67 ± 2.39 83.28 ± 2.47 75.99 ± 3.27 45.13 ± 1.85

a: untreated group-group treated with culture solution only

b: Probability p (Mann-Whitney U test with Holm-Bonferroni correction, Significant; * p <0.05)

Statistical analysis result of positive control group (SDS treatment group) X ² Degrees of freedom Significance density 42.061 4 <0.001

Probability p (kruskal-Wallis test)

Absorbance and Cell Viability (positive ± standard deviation) of positive control group (SDS treated group) Sample concentration (㎍ / mL)

0
(Untreated group ^a )
One
10
100
1000 Absorbance (OD 540) 1.208 ± 0.017 1.068 ± 0.023 0.229 ± 0.011 0.053 ± 0.003 0.051 ± 0.003 p-value ^b - <0.001 * <0.001 * <0.001 * <0.001 * Cell survival rate (%) 100.00 ± 0.00
88.48 ± 2.19 18.97 ± 0.95 4.39 ± 0.26 4.23 ± 0.20

a: untreated group-group treated with culture solution only

b: Probability p (Mann-Whitney U test with Holm-Bonferroni correction, Significant; * p <0.05)

3. Evaluation index 3: evaluation of antioxidant efficacy

1) Antioxidant Evaluation Test

Free radicals with free radicals cause damage to skin cells and tissues as a major cause of skin aging. Therefore, the elimination of free radicals can inhibit aging of the skin. Such antioxidant capacity of the sample can be evaluated by DPPH (2,2-Diphenyl-1-Picrylhydrazyl) assay. When DPPH, which is a relatively stable free radical, reacts with the sample and is reduced, a change in color from purple to yellow occurs, and the antioxidant activity of the sample can be confirmed. In this experiment, 100 μl of 0.15 mM DPPH solution prepared with methanol and ultrapure water were prepared in each well of a 96 well plate so that the final concentration was 1 μg / mL, 10 μg / mL, 100 μg / mL and 1000 μg / mL. 100 μl of each diluted sample SJ-DFE was added, and the light shielding reaction was performed at room temperature for 30 minutes, and then the absorbance of the reaction solution was measured at 540 nm using a microplate reader.

No treatment group: methanol + ultrapure water

Negative Control: 0.15 mM DPPH Solution + Ultrapure Water

Sample treatment group: 0.15 mM DPPH solution + sample solution diluted by each concentration

2) results

DPPH of samples SJ-DFE 1 μg / mL, 10 μg / mL, 100 μg / mL and 1000 μg / mL and ascorbic acid 1 μg / mL, 10 μg / mL, 50 μg / mL and 100 μg / mL as positive controls As a result of the assay test, absorbance and antioxidant capacity are shown in Tables 12 to 15 below, and the results are also graphically represented as shown in FIGS. 5 to 8.

SJ-DFE treated group showed antioxidant activity of 1.77% at 1 μg / mL, 23.67% at 10 μg / mL, 96.94% at 100 μg / mL, and 97.44% at 1000 μg / mL, respectively. There was a statistically significant difference (p <0.05) at 100 μg / mL and 1000 μg / mL treatment concentrations compared to the negative control.

Ascorbic acid treatment group used as a positive control showed 25.99% at 1 μg / mL, 81.51% at 10 μg / mL, 95.78% at 50 μg / mL and 95.89% at 100 μg / mL, respectively. Showed a statistically significant difference (p <0.05) in comparison with the negative control.

Statistical analysis result of SJ-DEF treatment group (comparison after concentration treatment) X ² Degrees of freedom Significance density 42.525 4 <0.001

Probability p (kruskal-Wallis test)

Absorbance and Antioxidant Capacity (Mean ± Standard Deviation) of SJ-DEF Treatment Group

Untreated group ^a

Voice control ^b SJ-DEF concentration (µg / mL) One 10 100 1000 Absorbance (OD 540) 0.036 ± 0.001 0.244 ± 0.004 0.240 ± 0.005 0.194 ± 0.004 0.042 ± 0.004 0.041 ± 0.001 p-value ^c - - 0.136 <0.001 * <0.001 * <0.001 * Antioxidant activity (%) 100.00 ± 0.00 0.00 ± 0.00 1.77 ± 1.63 23.67 ± 1.44 96.94 ± 2.02 97.44 ± 0.33

a: untreated group-methanol + ultrapure water

b: negative control: 0.15 mM DPPH solution + ultrapure water

c: Probability p (Mann-Whitney U test with Holm-Bonferroni correction, Significant; * p <0.05)

Results of statistical analysis of ascorbic acid treatment group (compared after treatment by concentration) X ² Degrees of freedom Significance density 40.981 4 <0.001

Probability p (kruskal-Wallis test)

Absorbance and Antioxidant Activity (Mean ± Standard Deviation) of Ascorbic Acid Treated Group

Untreated group ^a

Voice control ^b Ascorbic acid concentration (㎍ / mL) One 10 50 100 Absorbance (OD 540) 0.036 ± 0.001 0.244 ± 0.004 0.190 ± 0.005 0.074 ± 0.001 0.044 ± 0.001 0.044 ± 0.000 p-value ^c - - <0.001 * <0.001 * <0.001 * <0.001 * Antioxidant activity (%) 100.00 ± 0.00 0.00 ± 0.00 25.99 ± 2.74 81.51 ± 0.33 95.78 ± 0.44 95.89 ± 0.31

a: untreated group-methanol + ultrapure water

b: negative control: 0.15 mM DPPH solution + ultrapure water

c: Probability p (Mann-Whitney U test with Holm-Bonferroni correction, Significant; * p <0.05)

4. Evaluation index 4: evaluation of wrinkle improvement efficacy

1) Elastase inhibition assay

Using N-succinyl- (Ala) ₃ -p-nitroanilide as a substrate, the amount of p-nitroanilide produced at room temperature for 5 minutes was measured at 410 nm. That is, each test substance was prepared at each concentration, and 20 μl was taken in an epp.tube, and 100 μl of 1.6 mM N-succinyl- (Ala) ₃ -p-nitroanilide and 20 μl of 0.4 U / mL porcine pancreas elastase solution were used as a substrate. 60 μl of 10 mM tris-HCl buffer (pH 8.0) was added sequentially, followed by reaction at room temperature for 5 minutes, and the absorbance was measured at 410 nm to determine the elastase inhibition rate.

Elastase inhibition (%) = 100- (absorbance of sample added / absorption of no additive) × 100

2) results

end. Elastase Inhibitory Activity of Oleanolic Acid in Positive Control

Table 16 shows the results of elastase inhibitory activity confirming the positive control of oleanolic acid known to have antioxidant activity. As a result, it showed a concentration-dependent elastase inhibitory activity in the test concentration range (IC ₅₀ : 11.9785 ug / mL), it was confirmed that there is no problem in this test process (Fig. 9).

Elastase Inhibitory Activity of Oleanolic Acid Elastase inhibitory activity (% of control)
Statistical analysis (p-value) Concentration (ug / mL) Primary Secondary 3rd Average Standard Deviation 0 -0.04 -0.04 0.07 0.00 0.06 0.78125 18.34 17.38 18.02 17.91 0.49 0.0002 1.5625 26.51 27.26 24.07 25.95 1.67 0.0014 3.125 30.97 29.49 30.23 30.23 0.74 0.002 6.2 43.19 41.49 42.44 42.37 0.85 0.0001 12.5 68.67 66.97 71.43 69.03 2.25 0.0004 25 73.88 76.32 75.47 75.22 1.24 0.0001 50 79.29 77.59 76.85 77.91 1.25 0.0001

I. Elastase Inhibitory Activity of SJ-DFE Samples

The elastase inhibitory activity of the SJ-DFE sample was confirmed by using N-succinyl- (Ala) 3-p-nitroanilide as the substrate and porcine pancreas elastase as the enzyme source. (Table 17) As a result, the SJ-DFE sample was 1.0 It was confirmed that the concentration-dependent and statistically significant elastase inhibitory activity (IC ₅₀ : 661.228 ug / mL) in the range of to 1000 ug / mL (Fig. 10).

Elastase Inhibitory Activity of SJ-DFE Samples Elastase inhibitory activity (% of control)
Statistical analysis (p-value) Concentration (ug / mL) Primary Secondary 3rd Average Standard Deviation 0 -1.367989 -1.470588 2.838577 9.47E-15 2.458815 0.1 5.198358 5.506156 5.711354 5.471956 0.258202 0.0597 One 6.326949 11.86731 9.610123 9.268126 2.785966 0.0597 10 15.66347 23.35841 21.20383 20.07524 3.969673 0.0034 100 26.94938 39.05609 29.82216 31.94254 6.325748 0.0065 1000 60.80711 65.42408 63.16689 63.13269 2.308672 <0.001

5. Evaluation index 5: Evaluation of stability of raw materials

1) Accelerated test condition

① Lot selection: Use the same prescription, formulation and packaging as commercially available products. In principle, the test should be conducted on 3 lots or more, except when it is judged not to affect the stability.

② Preservation conditions: Appropriate test conditions should be set according to the distribution route and the characteristics of the formulation. Generally, the test is conducted at a temperature higher than 15 ℃ above the designated storage temperature of the long-term storage test. For example, 40 ± 2 ° C / relative humidity 75 ± 5% for room temperature cosmetics and 25 ± 2 ° C / relative humidity 60 ± 5% for cold storage cosmetics.

6. Evaluation index 6: oil and water measurement evaluation

This oil and moisture measurement evaluation is for a cream formulation (Example 4) formulated with the addition of a bone chain hydrothermal extract.

1) Skin oil improvement efficacy evaluation

① Test order

-The subject was informed about the test method, schedule, risk, and possible adverse reactions, completed basic information, and signed a consent form.

-After washing the face using the standard cleanser provided by the tester, tap water with a paper towel to remove water, and then, it was stabilized under constant temperature and humidity conditions (20-24 ° C, 40-60% RH) for 30 minutes.

The measuring device was measured before application of the sample to the test site.

-After the tester applied the test sample to the test site once, the amount of sebum after application of the sample was measured.

-The dermatologist will check for any adverse reactions (pruritus, irritation such as erythema).

② Evaluation and Analysis Method

-Sebum adsorption evaluation using Sebumeter: The change of sebum amount measured before and after sample application was compared by Sebumeter.

-Safety evaluation by dermatologist: During the test period, we evaluated the occurrence of side effects (erythema, edema, tearing, itching, burning, stiffness, tingling and other abnormalities) due to product use.

③ Statistical analysis method

The significance is checked using the Minitab 17 (Minitab® 17.3.1, Minitab Inc.) program.

-If the measured value of the test site is estimated to be normal distribution after the Ryan-Joiner Normality Test, verify the significance through the following statistical method.

-Before and after comparison: For the comparison of the measured value before and after, use the paired t-test at p <0.05 level. In case of repeated measurement more than 3 times, confirm the significance through one-way ANOVA.

-Comparison between groups: Welch's t-test is used to compare the measured values one time before and after the other. When the measurement is repeated three times or more, the significance is confirmed through two-way ANOVA.

-If normality is rejected, the non-parametric statistical method below confirms the significance.

-Before and after comparison: For the comparison of the values measured one time before and after, use Wilcoxon signed rank test. In case of repeated measurement more than three times, the Kruskal-Wallis test confirms the significance.

-Comparison between groups: The comparison of the values measured one time before and after each is done by Mann-Whitney U test. In the case of repeated measurement more than three times, the Friedman test confirms the significance.

-Check the significance using reasonable statistics such as MANOVA and ANCOVA.

All data are summarized as mean and standard deviation for continuous variables, and frequency and percentage for categorical variables.

④ Test result

Basic Information of Subject No. ID Gender Age No. ID Gender Age One 3108 Female 21 12 1918 Female 46 2 2733 Female 21 13 2715 Female 46 3 3106 Female 22 14 1719 Female 46 4 1930 Female 25 15 1675 Female 46 5 1956 Female 28 16 1749 Female 46 6 2639 Female 30 17 1385 Female 47 7 2854 Female 33 18 2939 Female 49 8 2793 Female 37 19 1533 Female 51 9 2528 Female 43 20 2450 Female 54 10 2515 Female 44 21 2155 Female 56 11 2173 Female 44

Subject's Basic Information-Summary Total number of subjects 21 people gender M: 0 Female: 21 Average age 40 years old Age distribution 20's 5 people 30 spaces 3 people 40 spaces 10 people 50 spaces 3 people

Pizza quantity-Sebumeter (Casual sebum level ㎍ / ㎠) ID
Test site Before application After application of sample 3108 55 63 2733 74 86 3106 42 128 1930 67 109 1956 98 251 2639 28 88 2854 82 124 2793 27 112 2528 99 125 2515 51 197 2173 30 84 1918 63 213 2715 34 67 1719 22 91 1675 96 152 1749 66 121 1385 98 199 2939 89 110 1533 94 164 2450 22 90 2155 23 66

Routine sebum level (㎍ / ㎠) regularity test (Ryan-Joiner Normality Test) division Average Standard Deviation N RJ P-value black Before application 60.0 29.03 21 0.961 0.099 Normal distribution After application 125.7 52.36 21 0.953 0.054 Normal distribution

Statistical sebum level (㎍ / ㎠) change statistical results, paired t test division
Observations
Daily sebum level change
T-value
P-value
Average Standard Deviation Before application-after application 21 -65.71 42.49 -7.09 <0.001

Sebum change
division Measures
Improvement
P-value
Before use after use Test site 60.00 125.71 ^*** 52.27% <0.001

*: p <0.05, **: p <0.01, ***: p <0.001

The test site to which the sample was applied once was increased to a statistically significant level (p <0.05) compared to before application, so that the test sample may temporarily help to improve skin oiliness (temporary, individual difference). has exist.)

11 and 12 are graphs of the skin sebum amount improvement trend test results, Figure 11 is a graph showing the degree of skin sebum reduction, Figure 12 is a graph showing the rate of skin sebum improvement.

Post-Product Survey No. Survey contents result One Do you think your face will get greasy after applying the product on your skin? 100% 2 What was the degree of stickiness after applying the product to the skin? 100% 3 What was the smoothness after applying the product to the skin? 100% 4 How moist was it after applying the product on the skin? 95% 5 How was your product color satisfaction? 100% 6 What was the fragrance of the product? 100% 7 Did you have any troubles such as skin irritation and itching while using the product? 100% 8 Are you satisfied with your use of the product? 100% 9 If this product is released at a reasonable price, is there a willingness to purchase? 95%

For each item, calculate the proportion of subjects who answered above 'normal'.

Dermatosis during the trial Erythema edema Inseol itch sting Burning Stiffness Tingling Other
Abnormalities Mild ○ ○ ○ ○ ○ ○ ○ ○ ○ Moderate ○ ○ ○ ○ ○ ○ ○ ○ ○ Severe ○ ○ ○ ○ ○ ○ ○ ○ ○

No unusual symptoms occurred during the trial.

⑤ Conclusion and Discussion

A total of 21 subjects were tested for skin oil improvement efficacy of the 'cream' sample, and all 21 subjects completed the test without dropping out.

As a result of measuring the instrument, the amount of sebum before application was 60.00 and the amount of pizza after application of test sample was 125.71. After application, the amount of skin sebum increased to a statistically significant level (P <0.05) compared to before application. I think it can help. In addition, no adverse events were observed in all subjects during the trial. In conclusion, the test site where the 'cream' sample was used once was found to have a statistically significant level (P <0.05) compared to before applying the sample, indicating that the test sample could temporarily improve skin oiliness. become. In addition, no special adverse reactions were observed during use, so it was considered safe (temporary, individual differences).

2) Evaluation of efficacy of improving skin moisture content

① Test order

-Fill out the Basic Information Questionnaire and Consent Form.

-After washing both face parts using the standard cleaner provided by the tester, tap water with a paper towel to remove water, and then stabilized at constant temperature and constant humidity (20-24 ° C, 40-60% RH) for 30 minutes.

The area of measurement area was 1/2 point of the imaginary line connecting both lip commissuure and ipsilateral tragus so that measurements could be made at the same site every time.

-The corneometer was measured five times and the average value of the measured values was recorded as the measured value.

-The test sample was applied to the test site, waited for 10 minutes in a constant temperature and humidity condition (Room temperature 22 ± 2 ℃, Relative humidity 50 ± 10%), and then the device was evaluated in the same way as before the application.

-A dermatologist confirmed the presence of adverse reactions (pruritus, irritation symptoms such as erythema).

② Evaluation Method

Evaluation of Moisturizing Improvement by Corneometer

-Changes in skin moisture content were measured using Corneometer before and after application of the measurement site, and the improvement rate was confirmed as follows.

Safety assessment by dermatologist

-During the trial period, each visited subject was evaluated for adverse events (erythema, swelling, tearing, itching, pain, burning, stiffness, tingling, and other abnormalities).

③ Statistical analysis method

The significance is checked using the Minitab 17 (Minitab® 17.3.1, Minitab Inc.) program.

-If the measured value of the test site is estimated to be normal distribution after the Ryan-Joiner Normality Test, verify the significance through the following statistical method.

-Before and after comparison: For the comparison of the measured value before and after, use the paired t-test at p <0.05 level. In case of repeated measurement more than 3 times, confirm the significance through one-way ANOVA.

-Comparison between groups: Welch's t-test is used to compare the measured values one time before and after the other. When the measurement is repeated three times or more, the significance is confirmed through two-way ANOVA.

-If normality is rejected, the non-parametric statistical method below confirms the significance.

-Before and after comparison: For the comparison of the values measured one time before and after, use Wilcoxon signed rank test. In case of repeated measurement more than three times, the Kruskal-Wallis test confirms the significance.

-Comparison between groups: The comparison of the values measured one time before and after each is done by Mann-Whitney U test. In the case of repeated measurement more than three times, the Friedman test confirms the significance.

-Check the significance using reasonable statistics such as MANOVA and ANCOVA.

All data are summarized as mean and standard deviation for continuous variables, and frequency and percentage for categorical variables.

④ Test result

Basic Information of Subject No. ID Gender Age No. ID Gender Age One 3108 Female 21 12 1918 Female 46 2 2733 Female 21 13 2715 Female 46 3 3106 Female 22 14 1719 Female 46 4 1930 Female 25 15 1675 Female 46 5 1956 Female 28 16 1749 Female 46 6 2639 Female 30 17 1385 Female 47 7 2854 Female 33 18 2939 Female 49 8 2793 Female 37 19 1533 Female 51 9 2528 Female 43 20 2450 Female 54 10 2515 Female 44 21 2155 Female 56 11 2173 Female 44

Subject's Basic Information-Summary Total number of subjects 21 people gender M: 0 Female: 21 Average age 40 years old Age distribution 20's 5 people 30 spaces 3 people 40 spaces 10 people 50 spaces 3 people

Moisture content in stratum corneum-Corneometer Value ID
Left test part Right test site Before application After application Before application After application 3108 50.00 67.60 50.30 65.40 2733 43.20 56.00 44.60 56.20 3106 40.00 57.00 60.80 61.20 1930 46.70 50.40 48.60 51.40 1956 39.00 61.60 41.80 55.40 2639 41.20 56.10 39.60 54.00 2854 33.21 49.50 30.60 48.50 2793 43.80 65.20 44.00 62.40 2528 37.40 78.00 58.00 68.40 2515 36.80 54.10 39.10 57.20 2173 42.80 48.50 40.40 49.60 1918 30.60 46.50 31.20 44.10 2715 54.60 69.20 53.40 67.80 1719 42.80 57.60 42.30 58.00 1675 46.00 65.20 42.00 69.20 1749 21.90 33.80 22.40 34.60 1385 55.40 70.20 54.20 66.80 2939 46.00 49.00 40.00 47.20 1533 58.20 68.20 55.20 66.80 2450 44.50 59.30 42.70 54.40 2155 36.60 41.50 35.00 42.30

Corneometer value Ryan-Joiner Normality Test division Average Standard Deviation N RJ P-value black left side
exam
part Before the test 37.79 7.168 21 0.976 > 0.100 Normal distribution After application 53.39 9.549 21 0.991 > 0.100 Normal distribution right
exam
part Before the test 38.75 8.371 21 0.985 > 0.100 Normal distribution After application 54.80 9.870 21 0.981 > 0.100 Normal distribution

Comparison statistics before and after corneometer value, paired t test division
Observations
Change in moisture content before and after application
T-value
P-value
Average Standard Deviation Left test site 21 15.24 9.87 7.41 <0.001 Right test site 21 13.03 9.94 7.57 <0.001

Changes in moisture content in the stratum corneum
division
Desquamation Index
Improvement
(Pre-application)
p-value
(Before and after application) Before application After application Left test part 41.61 56.85 ^*** 36.63% <0.001 Right test site 42.76 55.80 ^*** 30.47% <0.001

*: p <0.05, **: p <0.01, ***: p <0.001

The test site to which the test sample was applied once was increased to a statistically significant level (p <0.05) after application, and it is judged that the test sample has an effect of improving skin moisture content. )

13 and 14 are graphs showing changes in skin moisture content (Corneometer value), and FIG. 13 is a graph showing a trend of improving skin moisture content, and FIG. 14 is a graph showing a rate of improvement of skin moisture content.

Dermatosis during the trial Erythema edema Inseol itch sting Burning Stiffness Tingling Other
Abnormalities Mild ○ ○ ○ ○ ○ ○ ○ ○ ○ Moderate ○ ○ ○ ○ ○ ○ ○ ○ ○ Severe ○ ○ ○ ○ ○ ○ ○ ○ ○

No unusual symptoms occurred during the trial.

⑤ Conclusion and Discussion

A total of 22 subjects were tested for improving the skin moisture content of the 'cream' sample, and all 22 subjects completed the test without dropping out. Corneometer measurement (A.U.) at the left test site was 41.61 before application, 56.85 after application, and Corneometer measurement (A.U.) at the right test site was 42.76 before application and 55.80 after application. The stratum corneum moisture content improvement rate in the left test site was 36.63%, and the stratum corneum water content improvement rate in the right test site was 30.47%. After application of the test sample once, the stratum corneum moisture content increased to a statistically significant level (p <0.05), and it was confirmed that the test sample had an effect of improving skin moisture content. In addition, no specific adverse events were observed in all subjects during the trial.

In conclusion, the test site where the 'Cream' test sample was applied once has a statistically significant level (p <0.05) compared to that before the sample application, indicating that the test sample has an effect of improving skin moisture content. do. In addition, this sample is considered to be a safe sample because no special adverse reaction was observed in all subjects during the test period.

7. Evaluation index 7: Patch test evaluation

This patch test is for a cream formulation (Example 4), which was prepared by the addition of Bolchibobo hot water extract.

1) test method

① Subject Selection: 31 subjects who meet the selection criteria and do not meet the exclusion criteria

② Patch area: Patch on the subject's back for 24 hours

③ Observation: Observed 30 minutes, 24 hours, 48 hours after patch removal

④ Evaluation: Evaluation based on self-applied criteria applying International Contact Dermatitis Association (ICDRG) and American Cosmetic Association (PCPC) standards

2) criteria

Visual assessment method-Skin response after patch removal Visual assessment is based on the criteria of the International Contact Dermatitis Research Group (ICDRG) and the Safety Evaluation Guidelines of the Personal Care Products Council (PCPC). Evaluate accordingly.

Skin irritation scoring system score Criteria 0 No signs of inflammation, normal skin 0.5 Doubtful or slight reaction One Slight erythema 2 Moderate erythema with or without partial edema or papules 3 Moderate erythema with diffuse edema 4 Intense erythema with diffuse edema with veicles

Irritant classification Stimulus index Irritation evaluation 0 ≤ <0.02 No irritancy 0.02≤ <0.25 Hypoallergenic low irritancy 0.25≤ <1 Slight irritancy 1≤ <2.5 Moderate irritancy 2.5≤ Severe irritancy

3) results

Skin irritation index 0.000 points, irritant

The present invention comprises a cosmetic composition (Drynaria fortunei) alone or mixed with a natural antioxidant extract, natural skin stability, moisturizing extract to provide a cosmetic with excellent antioxidant and skin stability effect, Industrial application is great as it provides a multifunctional cosmetic by presenting a formulation having excellent formulation stability.

Claims (9)

delete It is composed of a mixture of 1.0 ~ 99.0 wt% bone extract extract and 1.0 ~ 99.0 wt% natural antioxidant extract,
The natural antioxidant extract,
The vortex is added to distilled water, and the vortex and the distilled water are added in a weight ratio of 1:15 to 30, extracted with cooling under reflux at 65 to 80 ° C. for 4 to 7 hours, and the extracted solution is filtered with filter paper (Whatman No. 2). After filtering, the filtrate was concentrated to a temperature of 55 ~ 65 ℃ using a rotary decompression concentrator 10 ~ 30 wt% obtained by extracting;
To dry spirit (Smilacis glabrae Rhizoma, SG) is added to the distilled water, the bokbokyeong and distilled water is added so as to make a weight ratio of 1: 5 to 10, extracted with reflux cooling at 65 ~ 80 ℃ for 4 to 7 hours, The extracted solution was filtered through a filter paper (Whatman No. 2), and then the filtrate was concentrated using a rotary pressure reducer at a temperature of 55 to 65 ° C., 10 to 30 wt%;
Green tea is added to the distilled water at 65 ~ 75 ℃, the green tea and distilled water is added so as to make a weight ratio of 1: 15 to 25, 10 ~ 30 wt% green tea extract obtained by extracting for 10 to 30 minutes in a 70 ℃ water bath;
Rice bran powder is added to distilled water, and the rice bran powder and distilled water are added in a weight ratio of 1:20 to 30, heated to 100 ° C., extracted for 3 to 5 hours, and the extracted solution is filtered with filter paper (Whatman No. 2). Then, 10 ~ 30 wt% of rice bran extract obtained by concentrating the filtrate at a temperature of 55 ~ 65 ℃ using a rotary pressure reducer;
Grape seed powder was added to 70% (v / v) methanol, and the grape seed powder and methanol were added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution was filtered through a filter paper (Whatman No. 2), the filtrate was concentrated by using a rotary decompression concentrator at a temperature of 55 ~ 65 ℃ 10 ~ 30 wt%;
Flaxseed powder was added to 70% (v / v) methanol, and the flaxseed powder and methanol were added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution was filtered through a filter paper (Whatman No. 2), and then the filtrate was concentrated to a temperature of 55 ~ 65 ℃ using a rotary pressure reducer 10 ~ 30 wt%; And
Pomegranate seed powder is added to 70% (v / v) methanol, and the pomegranate seed powder and methanol are added in a weight ratio of 1: 8 to 15, and extracted at 50 to 70 ° C. for 5 to 7 hours, and the extracted solution is filtered through After filtering by (Whatman No. 2), the filtrate was concentrated by using a rotary pressure reducer at a temperature of 55 ~ 65 ℃ 10 ~ 30 wt%; Pomegranate seed extract; Cosmetic composition using a bone clabo extract.
delete The method according to claim 2,
Bolchibori extract is added to distilled water, but the mixture of boulbori and distilled water is added so that the ratio of 1: 8 to 15 by weight, to form a mixture,
The mixture is extracted by applying 80 ~ 95 ℃ heat for 2-4 hours,
The extracted solution was filtered through filter paper and concentrated using a rotary pressure reducer.
Drying the concentrate with a freeze-drier and then pulverized cosmetic composition using a bone clabo extract, characterized in that the powder was prepared.
Cosmetics comprising a golbobo extract, characterized in that the cosmetic composition of claim 2 comprising 1.0 to 50.0 wt% of the total weight of the cosmetic.
1.0 to 50.0 wt% of the cosmetic composition of claim 2;
Water 85.0 ~ 92.0 wt%, Disodium EDTA 0.01 ~ 0.05 wt%, Glycerin 2.5 ~ 8.5 wt%, Butylene Glycol 1.5 ~ 7.5 wt%, 10.0 sodium hyaluronate 1% Solution 49.0-90.0 wt% of 100.0 wt% of the first composition composed of 0.2-3.5 wt% of a mixture; And
Alcohol 73.0 ~ 79.0 wt%, 1,2-hexanediol 15.0 ~ 23.0 wt%, PIG-40 Hydrogenated Castor Oil 1.5 ~ 6.5 wt %, Fragrance (Fragrance) 100.0 wt% of the second composition 1.0 ~ 15.0 wt% of the composition consisting of a mixture of 0.1 to 2.5 wt%; a cosmetic comprising a bone clavicle extract, characterized in that the skin is composed of a mixture of.
1.0 to 50.0 wt% of the cosmetic composition of claim 2,
Water 55 ~ 65 wt%, Disodium EDTA 0.01 ~ 0.1 wt%, Dipotassium Glycyrrhizate 0.05 ~ 0.2 wt%, Butylene Glycol 20 to 35 wt%, Glycerin 2.5 to 12.5 wt%, Allantoin 0.05 to 0.5 wt%, 1% Sodium Hyaluronate 1% Solution 1.0 to 8.5 wt%, Beta-Glucan -Glucan) 35.0 to 65.0 wt% of a third composition composed of a mixture of 0.01 to 0.08 wt% and 1,2-Hexanediol 1.5 to 7.5 wt%;
2.0 to 15.0 wt% of a fourth composition formed by mixing 98.0 to 99.8 wt% of purified water and 0.2 to 2.0 wt% of Xanthan Gum;
10.0 to 30.0 wt% of a fifth composition formed by mixing 98.0 to 99.8 wt% of purified water and 0.2 to 2.0 wt% of Carbomer;
9 to 99.0 wt% of purified water, 2.0 to 15.0 wt% of a sixth composition formed by mixing 1.0 to 5.0 wt% of triethanolamine; And
Composition 7 composed of a mixture of PIG-60 Hydrogenated Castor Oil 2.0 to 9.5 wt%, Alcohol 89.0 to 97.0 wt%, Fragrance 0.2 to 1.5 wt% 0.5 ~ 10.5 wt%; Cosmetics comprising the extract of bone bolster, characterized in that the composition is an essence of the mixture.
1.0 to 50.0 wt% of the cosmetic composition of claim 2,
Water 42.0 ~ 48.0 wt%, Disodium EDTA 0.01 ~ 0.08 wt%, Fiji / Fiji-17 / 6 Copolymer (PEG / PPG-17 / 6 Copolymer) 0.05 ~ 0.5 wt %, Glycerin 29.0 to 37.0 wt%, DL-Panthenol 3.5 to 12.0 wt%, Allantoin 0.05 to 0.5 wt%, Biosaccharide Gum-1 2.0 to 20.0 to 55.0 wt% of an eighth composition composed of a mixture of 10.5 wt%;
0.1 to 5.0 wt% of a ninth composition formed by mixing 90.0 to 97.0 wt% of butylene glycol and 3.0 to 10.0 wt% of Xanthan Gum;
10.0 to 30.0 wt% of the 10th composition, which is formed by mixing 96.5 to 99.8 wt% of purified water and 0.2 to 3.5 wt% of hydroxyethylcellulose;
10.0 to 30.0 wt% of the 11th composition formed by mixing 96.5 to 99.8 wt% of purified water and 0.2 to 3.5 wt% of Carbomer;
3.0 to 20.0 wt% of the twelfth composition formed by mixing 96.5 to 99.5 wt% of purified water and 0.5 to 3.5 wt% of triethanolamine; And
PIG-60 Hydrogenated Castor Oil 2.5 to 7.5 wt%, 1,2-Hexanediol 23.0 to 30.0 wt%, Alcohol 62.0 to 74.0 wt %, Fragrance (Fragrance) cosmetic composition comprising a bone clavicle extract, characterized in that the mask sheet is composed of a mixture of 1.5 to 15.5 wt%;
1.0 to 50.0 wt% of the cosmetic composition of claim 2,
Cetyl PEG / PPG-10 / 1 Dimethicone 22.0 ~ 35.0 wt%, Cyclopentasiloxane 42.0 ~ 48.0 wt%, Dimethicone 23.0 ~ 30.0 wt 3.0 to 20.0 wt% of the 14th composition constituted by a mixture of%;
Water 85.0 ~ 93.0 wt%, Glycerin 2.5 ~ 8.5 wt%, Dipropylene Glycol 1.5 ~ 6.5 wt%, Sodium Chloride 0.2 ~ 2.5 wt% 45.0 to 95.0 wt% of the 15th composition; And
Waterdrop cream composed of a mixture of 0.1-7.5 wt% of the 16th composition, which is a mixture of 95.0-99.0 wt% of 1,2-hexanediol and 1.0-5.0 wt% of fragrance; Cosmetics comprising a bone clavicle extract, characterized in that.

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