KR101919113B1 - Composition comprising Nelumbo Nucifera callus extract for reinforcing skin barrier - Google Patents

Abstract

The present invention relates to a composition for reinforcing the skin barrier, which contains a Nelumbo nucifera callus extract, or a mixture of the Nelumbo nucifera callus extract and a machilia extract. The Nelumbo nucifera callus extract and the machilia extract promote the synthesis of claudin-1 and claudin-4, which constitute tight junctions of the keratinocyte layer, and thus is effective in strengthening the skin barrier damaged by external factors. When the Nelumbo nucifera callus extract and the machilia extract are used after being mixed at a predetermined ratio, a synergistic effect on strengthening the skin barrier by promoting the synthesis of claudin-1 and claudin-4, respectively, can be exhibited more than when using each of the same alone.

Description

[0001] The present invention relates to a composition for enhancing skin barrier containing a callus extract,

The present invention relates to a composition for enhancing skin barrier comprising an extract of soft callus, and more particularly to a composition having a skin barrier strengthening effect including a soft callus extract or a combination of soft callus and extract extract, To a cosmetic composition.

The skin performs various functions essential for the human body to survive. Barrier function to maintain homeostasis in the body in response to environmental changes, sensory function to recognize external changes, and body temperature control are among the most representative functions of the skin. All of these functions correspond to changes in the external environment, And maintains the homeostasis capable of maintaining the same. Among the various functions of the skin, especially the barrier function of the skin, is mainly caused by the stratum corneum which is located at the outermost part of the skin. However, recent studies have shown that the stratum corneum has been reported to affect not only the barrier function but also the function, role, and structure of the inner living cell layer, i.e., the epidermis or dermis, and its importance is continuously increasing . As the importance of the stratum corneum is emphasized, researches on it have been actively carried out. In particular, studies on the structure and function of the keratinocytes in the stratum corneum have been actively carried out.

There are two types of pores associated with the water homeostasis present in the epidermis: aquaporins (AQP) and tight junctions (TJ). Transport of water and solvents exists in a transcellular way through cells through the aquaporin and a paracellular way through the extracellular space through tight junctions.

Among these, tight junctions are located in the intercellular spaces of the epidermal granule cell layer and are located at the outermost part of the intercellular junctions (ICJ) structures, that is, at the top of the epidermis. And it is beneficial to maintain the homeostasis of the human body. Such tight junction structures include tight junction-associated proteins such as occludin (OC), claudin, and junctional adhesion molecules. They are mediated by zona occludens-1 and cingulin, the plaque proteins present in the cytoplasm and actin cytoskeleton and cytosolic regulatory elements proteins and has a biological barrier function that regulates the adhesion of cells and the movement of water-containing solutes and water through the pericellular space. These tight junctions and tight junction-proteins thus serve as normal barriers to prevent transepidermal water loss (TEWL), a water loss through the stratum corneum in healthy skin, Or is up-regulated when injured. So it acts as a rescue system.

In the past, moisturizers were used to increase the moisture content of simple skin stratum corneum by using humectants that absorb moisture and occlusive moisturizers that prevent water evaporation. However, this is to simply attach a substance having a property of absorbing moisture to the skin horny layer lacking moisture or to form a film capable of preventing moisture evaporation, so that the skin horny layer can retain moisture for a long time, The ability of the skin to retain its water content was only a temporary measure to return to its previous state.

In order to overcome the limitations of the past moisturizing agents, researches have been actively conducted to induce a natural moisture homeostasis effect by the normal functioning of the skin barrier through reinforcement of the skin barrier. Skin barrier enhancement is a method that can fundamentally solve symptoms of lack of moisture (dryness) due to skin barrier function by restoring damaged and destroyed skin barrier by physical and chemical stimulation of external environment and further strengthening it, It is regarded as a more advanced type of function that is completely different from the moisturizing concept of.

Accordingly, the inventors of the present invention have found that Nelumbo Nucifera callus extract is effective as an anti-obesity, antioxidant, whitening, anti-wrinkle, anti-inflammatory, anti-cancer agent , And cell protection. However, the effect of skin barrier enhancement was confirmed by paying attention to no research on skin moisturizing. As a result, it was confirmed that the yeast callus extract promotes the synthesis of claudin - 1 and claudin - 4 among the elements that tight junction is strengthened.

Furthermore, the present inventors confirmed that synergistic effects of promoter synthesis of claudin-1 and claudin-4 were synergistically more effective than the case of using only calli extract or succulent extract alone by mixing the extract of Yakitori with the extract of Yakitori, To complete the reinforcing composition.

Accordingly, a problem to be solved by the present invention is to provide a composition for skin barrier enhancement comprising a kite callus extract or a combination of starter callus and starch extract.

Another object to be solved by the present invention is to provide a skin external preparation for skin barrier strengthening comprising a kite callus extract or a combination of kite callus and rosemary extract.

Another object to be solved by the present invention is to provide a cosmetic composition for enhancing skin barrier comprising a kite callus extract or a combination of kite callus and extract.

According to one aspect of the present invention, there is provided a composition for skin barrier enhancement comprising as an active ingredient a soft callus extract.

According to still another aspect of the present invention, there is provided a composition for skin barrier enhancement comprising, as an active ingredient, Yeast callus and a sugar-extracted extract mixture, as an additional ingredient.

Hereinafter, the present invention will be described more specifically.

The present invention provides compositions for enhancing skin barrier having promoting effects of claudin-1 and claudin-4 synthesis, including kite callus extracts or kite callus and rosemary extract mixtures.

Kite is a perennial plant that grows in a pond. The roots stretch to the side, are columnar, have many nodes, and the ends are particularly thick in autumn. The leaves come out from the rhizome and rise high on the water, close to a circle, veins spread all the way, about 40 cm in diameter, are not wetted by water, and there are spiny protrusions on the petiole have. In July ~ August, red, white flowers bloom, 15 ~ 20 cm in diameter, flower buds have thorns like petiole and one flower at the end. In October, the fruit is ripe, the nuts are oval, and the length is about 2 cm. The seeds of the fruit are seeded with green leaves, the green seeds of the mature seeds are green with the lenticular seeds, the white seeds of the seeds of mature seeds are cultivated in the livestock, The root of the root, the root of the root, and the root of the root of the root. It is well known that it is used for diarrhea, headache, dizziness, hematemesis, postpartum hemorrhage treatment, enuresis, and detoxification.

 Callus refers to a specific tissue or cell mass that is produced when a tissue cut from a plant is cultured in a medium containing auxin, a wound is injured to some kind of plant, or a wound is treated with auxin. Usually callus is an amorphous tissue or cell mass that has lost its ability to cause normal organogenesis or tissue differentiation, and is mostly flowcytosed. In a broad sense, it also includes plant tumor tissue caused by infection such as agrobacterium. Callus can be derived from any tissue, including the roots, stems, and leaves of plants. In the present invention, the callus derived from the soft petal leaf is used, but the present invention is not limited thereto.

The kite callus is derived from a seed of kite called arachidon, but is not limited thereto. Ara Hongryun refers to the germination and budding of Yeonan in 700 years after being excavated from Sungsan Mountain in Haman, Gyeongsangnam-do. It is also called Haman Lotus. Ara Hongryn is a long, petal-purple flower with a long petal that can be seen in the feuds of the Goryeo Dynasty. Each time the petal is rolled up again, its color gradually becomes thinner, and later it is characterized by a dark purple color remaining only at the end of the petal. The pure white color of the petal roots gradually fills the petals, and the long, elegant shape of the long petals is proud of its unrivaled shape.

Magnolia bark is a deciduous arboreous tree belonging to Magnoliaceae. It has the efficacy of dryness suppression and hajiman removal, ), Tibia (脘 肥 瀉泻), food gas (食品 气 滯), constipation (腹 便 constipation), and healing 痰 飮 (喘 咳) is treated. Examples of ingredients of the pasta include essential oils such as alpha, beta and gamma-oidesmol, magnolol, honokiol, tetrahydromagnolol, (magnocurarine), magnoflorine, obovatol, obovataldehyde, saponin and the like, and the indicator material of the bean sprouts is determined as "magnolol" by "quality standardization research for herbal medicinal herbs" (Korea Food & Drug Administration, 1996).

The tight junction is located in the intercellular space of the epidermal granule cell layer and is located at the outermost of the intercellular junctions (ICJ) structures, that is, at the top of the epidermis, and protects the internal organ from the external environment It plays a beneficial role in maintaining the homeostasis of the human body. Such tight junction structures include tight junction-associated proteins such as occludin (OC), claudin, and junctional adhesion molecules. These are actin cytoskeletons and cytoplasmic regulatory protein components, mediated by zona occludens-1 (ZO-1) and cingulin, the plaque proteins present in the cytoplasm. Bind to cytosolic regulatory proteins and have a biological barrier function that regulates cell adhesion and the migration of water-containing solutes and water through the periplasmic space.

The clathin-1 and clathin-4 are clathin proteins which are typical protein components constituting tight junctions. They are present in the granular layer of the skin (Stratum granulosum), and play a role of forming a skin barrier and maintaining function . It is known that claudin-1 and cladin-4 increase production as the differentiation of keratinocytes in the epidermis of the skin barrier progresses, and lesions and non-lesions of skin barrier-related diseases such as atopic dermatitis and psoriasis It is known that the production is abnormal or decreased in comparison with healthy skin at the site. Thus, increased protein production on claudin-1 and claudin-4 in skin barriers means that normal skin barrier functions such as skin protection from external factors and prevention of moisture loss in the skin are enhanced.

The skin barrier strengthening means strengthening the barrier function of the stratum corneum at the outermost part of the skin. The stratum corneum, which is the primary barrier of the skin, can be damaged by physical irritation or scars. In the case of people with atopic dermatitis or dry skin, the skin barrier of the stratum corneum is not functioned properly or is damaged. Soothing moisturizers are applied to reduce the treatment or damage of atopic and dry skin. Normal moisturizers can not solve fundamental problems by simply replenishing water or covering the skin. Therefore, in this aspect, the combination of the callus and rosemary extract improves the skin barrier function by solving the fundamental problem by increasing the expression of factors related to the barrier function of the stratum corneum.

The pale flower cell culture powder of the present invention can be obtained by crushing or extracting callus derived from soft flower petal.

The open calli extract of the present invention can be extracted by any extraction method known in the art. For example, purified water, C1 to C4 lower alcohols or a mixed solvent thereof. Specifically, it can be obtained by hot water extraction for 4 hours or more in purified water of 100 degrees or more.

The skin barrier enhancing composition of the present invention may contain 0.001 to 30% by weight, more preferably 0.001 to 10% by weight, and more preferably 0.01 to 10% by weight, based on the total weight of the extract, To 5.0% by weight, based on the total weight of the composition. When the dry callus extract is less than 0.001% by weight based on the total weight of the composition, the skin barrier strengthening effect is insignificant. When the dry caller extract is more than 30% by weight, the increase in skin barrier strengthening effect is not economical.

The starter callus and starch extract mixture of the present invention can be obtained by extracting and combining starch callus and starch with an appropriate solvent, respectively, in an appropriate manner, or by mixing starch callus and starch together and extracting them into a solvent at the same time.

The callus and sugar extract of the composition for skin barrier strengthening according to the present invention may be used in an amount of 0.1: 1 to 10: 1, preferably 0.1: 1 to 5: 1, more preferably 0.5: 1 to 2.5: : 1. ≪ / RTI >

The composition for enhancing skin barrier of the present invention may comprise 0.001 to 30% by weight, more preferably 0.001 to 10% by weight, of dry callus and sugar-extracted extract mixture in terms of dry weight by weight of the total composition, Preferably 0.01 to 5.0% by weight, but is not limited thereto. When the dried callus and sugar beet extract mixture is less than 0.001% by weight based on the total weight of the composition, the skin barrier hardening effect is insignificant. When the dry calli extract is more than 30% by weight, not.

The skin barrier enhancing composition of the present invention has an effect of increasing the expression of clathrin-1 and clathrin-4 in tight junctions.

The external preparation for skin or cosmetic composition of the present invention is not particularly limited in its formulation, and may include a cosmetic composition composition commonly used in the technical field to which the invention belongs according to the formulation to be produced. The cosmetic composition of the present invention can be manufactured into formulations such as lotion, milky lotion, nutritional cream, pack, essence, essence and the like. The emulsion, the emulsifier, the emulsion stabilizer, the moisturizer, the thickener, Can be selected and mixed.

FIG. 1 is a graph showing the amount of clathrin-1 produced according to the concentration of each of the callus extract and the backbone extract and the amount of the callus-1 extract mixture according to the mixing ratio.
FIG. 2 is a graph showing the amount of clathrin-4 produced according to the concentration of each of the callus extract and the backbone extract and the amount of the callus and callus extract mixture.
FIG. 3 is a graph showing the water loss of the hard skin caused by the application of the formulation of the starch callus extract and the mixture of the extracts of the backbone.

Hereinafter, the present invention will be described in detail with reference to Production Examples, Examples and Experimental Examples.

However, the following Production Examples, Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by Production Examples, Examples and Experimental Examples.

Production Example 1. Preparation of Yeast Callus extract

1-1) Induction of Kite callus

Nutrient- treated seeds of Nelumbo Nucifera were cut with a sharp knife to a depth of 3 to 5 cm to obtain a solution containing 40.0 g / L of sucrose, 5 to 10.0 g / L of agar, a-naphthaleneacetic acid (NAA) 0.5 mg / L, and 1.0 mg / L 6-benzylaminopurine (BAP) at pH 5.8. After about two weeks, shoots were formed from calli from calli, and callus was maintained by subculture with a route selection medium.

1-2) Preparation of Yeast Callus extract

The induced callus was dried in liquid nitrogen, finely ground, dissolved in purified water, and then extracted with hot water for more than 4 hours in purified water of 100 degrees or more. The resulting callus was lyophilized to obtain a soft petal cell culture powder, Example 1) was prepared.

Production Example 2. Preparation of extract

The dried pak bark was pulverized and then subjected to CO2 extraction to obtain a commercially available product (Cosphaderm, Magnolia Extract 98 (Example 2)) of a bark extract (Example 2) having a content of Magnolol and Honokiol of 49% , Cosphatec GmbH) was purchased and used.

Preparation Example 3. Preparation of a mixture of extract of callus and sugar extract

The extracts of the callus extract and the extract of the latex extract were 0.5: 1, 1.25: 1 and 2.5: 1, respectively, on the dry weight basis, using the extract of Calli extract (Example 1) 1 to obtain a mixture.

Sample Yeast callus extract: Mixing ratio of extract of hippopotamus extract (dry weight basis) Example 3 0.5: 1 Example 4 1.25: 1 Example 5 2.5: 1

EXPERIMENTAL EXAMPLE 1 Measurement of Accelerating Effect of Claudin-1 and Claudin-4 Synthesis

Yeast callus extract and starch extract were treated at a concentration without cytotoxicity. In the case of the mixture, the callus extract and starch extract were mixed at a ratio of 0.5: 1, 1.25: 1, 2.5: 1 The mixture of the mixed examples 3 to 5 was treated with the cells. The amount of claudin-1 and cludin-4 production was measured as follows.

HEKn (Human Epidermal Keratinocytes, neonatal), a keratinocyte, was added to the medium (8 × 10 ⁵ cells / dish) and cultured in a 5% CO 2 cell incubator at 37 ° C. for 48 hours. After 48 hours, Examples 1 to 5 were treated for each concentration as shown in Table 2 below, followed by culturing for 24 hours, and then proteins were extracted from the cells.

Protein quantification was performed using the Bradford reagent from Bio-Rad to determine the amount of cladin-1, cladin-4 protein expression in the same amount of protein by gamma-globulin standard curve. The same amount of protein was separated by electrophoresis on a 4-20% SDS-polyacrylamide gel, and the protein was transferred to a polyvinylidene fluoride membrane (PVDF membrane), and then clodin-1 and cladin- Bind to the existing claudin-1, claudin-4 protein site. In addition, the band intensity for cladin-1 and cladin-4 protein, which was obtained by treating horse-radish peroxidase (HRP) antibody after treatment with a substrate for HRP, To confirm the amount of protein production.

Sample Concentration in medium (ug / ml) Increase relative to control Claudin-1 (%) Claudin-4 (%) Control group 0.0 0.0 Example 1 (X) 5 2.7 3.4 12.5 20.6 60.2 25 30.9 225.1 Example 2 (Y) 10 20.1 25.6 Example 3 15 8.1 72.3 Example 4 22.5 35.4 200.9 Example 5 35 70.6 420.1 Efficacy predicted by Colby method (E) Example 3 22.3 28.1 Example 4 36.6 70.4 Example 5 44.8 193.1

As can be seen from the results of Example 1 and Example 2 in Table 2, it can be confirmed that the amounts of clone 1 and cladin-4 were increased compared with the control group when used alone or in combination.

In addition, a Colby's Equation method was used to predict the synergistic effect of the mixture to determine if there is a synergistic effect of the callus and extract extract mixture (Ferry, DESIGN AND ANALYSIS OF BIOLOGICAL ASSAYS OF MIXTURES, 2005 Reference). If the result of the actual mixture is larger than the predicted value of Colby, the synergy effect is considered.

[Equation 1]

The result predicted when there is no synergy or antagonism in the mixture (p + q g / L) of A (p g / L) and B (q g / L)

(E) = X + Y - X * Y / 100

X: Result value when using A (p g / L) alone

Y: Result value when using B (q g / L) alone

As can be seen from the results of Examples 3 to 5 and the Colby predictions in Table 2, there was a synergistic effect in Example 5 for the amount of claudin-1 production and a synergistic effect in Examples 3 to 5 for claudin- . In particular, the synergistic effect of Example 5, in which 2.5% by weight of callus extract and 2.5%

Formulation Example 1: Preparation of Solubilized Formulation

The solubilized formulations (Formulation Examples 1-1, 1-2, and 1-3) of Formulation Example 1, each containing a mixture of the callus extract and the backbone extract of Examples 3 to 5, were prepared as shown in [Table 3] below.

Prize ingredient content (%) Awards Purified water To 100 glycerin 10-25 Dipropylene glycol Betaine D-Panthenol Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Available image PIGGY-60 Hydrogenated Castor Oil 0 to 2 PIGGY-40 Hydrogenated Castor Oil 0 to 2 Polyhydric alcohol 0 to 10 Spices Suitable amount ethanol 0 to 20 Addition I Examples 3, 4 or 5 0.01 to 5 Addition II Preservative Suitable amount Other additives Suitable amount

<Manufacturing Method>

(1) The water phase and the usable image were uniformly mixed and dissolved respectively.

(2) The soluble image was put in the water phase and mixed and solubilized.

(3) Addition I was solubilized into a phase containing the mixture of Example 3, 4 or 5, followed by addition and mixing after addition and mixing.

Formulation Example 2: Preparation of Essence Formulation

(Formulation Examples 2-1, 2-2, and 2-3) of Formulation Example 2, which each consisted of the callus extract and the aftercoat extract mixture of Examples 3 to 5, were prepared as shown in Table 4 below.

Prize ingredient content (%) Awards Purified water To 100 Cetearyl-6 olivate 0.1 to 3 glycerin 10-25 1,2-propanediol Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Paid FAGE -100 stearate 0.1 to 1 Glyceryl stearate 0.1 to 1 Polysorbate 60 0.1 to 1 Cetyl alcohol 0.1 to 1 Behenyl alcohol 0.1 to 1 Squalane 5-20 Tocopheryl acetate 0.1 to 0.5 Addition I Examples 3, 4 or 5 0.01 to 5 Addition II incense Suitable amount Preservative Suitable amount Other additives Suitable amount

<Manufacturing Method>

(1) The water phase and the oil phase were warmed and homogeneously mixed and dissolved respectively.

(2) The oil phase was added to the aqueous phase at 75 DEG C, and the mixture was emulsified

(3) Additive Phase I: The additive Phase II was mixed after the mixture containing the mixture of Example 3, 4 or 5 was added as an active ingredient at 50 캜 and mixed.

Formulation Example 3: Preparation of cream formulations

The cream formulations (Formulation Examples 3-1, 3-2 and 3-3) of Formulation Example 3, each containing the soft callus extract and the aftercoat extract mixture of Examples 3 to 5, were prepared as shown in Table 5 below.

Prize ingredient content (%) Awards Purified water To 100 glycerin 10-25 Betaine Sodium hyaluronate Incrementer Suitable amount Metal ion sequestrant Suitable amount Paid FAGE -100 stearate 0.1 to 2 Glyceryl stearate 0.1 to 2 Polysorbate 60 0.1 to 2 Stearic acid 0.1 to 2 Cetearyl alcohol 0.1 to 2 Capric caprylic triglyceride 10 to 30 Tocopheryl acetate 0.1 to 0.5 Addition I Examples 3, 4 or 5 0.01 to 5 Addition II incense Suitable amount Preservative Suitable amount Other additives Suitable amount

<Manufacturing Method>

(1) The water phase and the oil phase were warmed and homogeneously mixed and dissolved respectively.

(2) The oil phase was added to the aqueous phase at 75 DEG C, and the mixture was emulsified

(3) Additive I was added at 50 캜 to a phase containing the mixture of Example 3, 4 or 5 as an active ingredient, and the additive phase II was mixed after mixing.

Experimental Example 2. Confirmation of stability of cosmetic composition

In order to confirm the stability of the formulations containing the combination of starter callus extract and backpack extract, the solubilized formulations, the essence formulations and the cream formulations of Formulation Example 1-3, Formulation Example 2-3, and Formulation Example 3-3 were added at 4 ° C , 30 ° C, 45 ° C and daylight for 12 weeks, and then color, texture and formulation changes were observed by visual evaluation and sensory evaluation. The results are shown in Tables 6 to 8 . At this time, the degree of change in color and coating form was classified and evaluated according to the following evaluation criteria.

<Evaluation Criteria>

No change: ○

Some changes: △

Significant change: ×

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time Formulation Example 1-3 (Solubilized Formulation) Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

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time Formulation Example 2-3 (Essence Formulation) Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

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time Formulation Example 3-3 (cream formulation) Bite change Color change Formulation change 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 4 ℃ 30 ℃ 45 ° C daylight 1 week ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 8 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ 12 weeks ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

As a result, as shown in Tables 6 to 8, it was confirmed that both the solubilized formulation, the essence formulation and the cream formulation had excellent stability under high temperature and daylight conditions.

Experimental Example 3. Measuring effect of enhancing skin barrier function

Transepidermal water loss (TEWL) was measured for Formulation Examples 3-1 to 3-3 of the cream type with different mixing ratios in order to confirm whether the annual callus and rosemary extract mixture actually enhanced skin barrier function Respectively. The light skin moisture loss measurement measures the amount of water loss (g / m ² h) through the stratum corneum in the dermis, and the lower the TEWL value, the better the skin barrier function. It is used to indirectly measure the state of the stratum corneum and the extent to which the skin barrier function is restored.

Twenty healthy adults with no history of illness or drug use that could affect the test were included. The age ranged from 20 to 55 years with an average age of 31.636 years.

The total duration of the experiment was 14 days. Subjects were asked to wash their forearms with soap, wipe them with physiological saline, dry them naturally, and square the 4 x 4 cm square symmetrically on the forearm, and then measure the TEWL of the skin surface. After measuring the TEWL before application, the skin barrier was damaged by proceeding with sodium lauryl sulfate (SLS) patch, and TEWL was again measured. Thereafter, 0.5 ml of the experimental group (Formulation Examples 3-1 to 3-3) was uniformly applied to the marked parts of the left forelimb, and the control arm (placebo cream) was applied to the right forelimb. Each cream prescription was applied 3 times a day for 2 weeks and the TEWL of the skin surface was measured at intervals of one week during application.

In each TEWL measurement, the measurement site was exposed in a constant temperature and humidity chamber having an average temperature of 25.0 ° C and an average humidity of 50%, and then the sample was stabbed for 30 minutes. Thereafter, an evaporimeter Tewameter TM 210 (Courage + Khazaka, Cologne, Germany ).

The measurement results for 14 days are shown in Table 9.

Sample -1 day
(Before application) 0 days
(Immediately after papermaking) 14 days Reduction rate Control group 7.782 ± 2.0 45.154 + - 22.4 18.451 ± 9.3 -26.703 + - 13.1 Formulation Example 3-1 7.601 + - 2.4 46.131 ± 20.3 17.851 + - 7.1 -28.280 ± 13.2 Formulation Example 3-2 7.720 ± 1.3 45.874 ± 21.6 15.343 + - 8.2 -30.531 + - 13.4 Formulation Example 3-3 7.760 + 1.7 47.031 + - 25.3 12.127 + - 4.7 -34.904 ± 20.6

As can be seen in Table 9, the mean amount of change of TEWL before and after use of the cream after SLS pellet was -26.703 ± 13.1 in the control group, -28.280 ± 13.2, -30.531 ± 13.4 in Formulations 3-1 to 3-3, 34.904 ± 20.6, indicating that the amount of decrease in TEWL in all experimental groups is larger than that in the control group. In particular, it was confirmed that the decrease in TEWL was 14 days after use of the cream of Formulation Example 3-3 with a 2.5: 1 mixture ratio of callus and hornblende extracts was the largest, which indicates that Formulation Example 3-3 reduces moisture loss Is the largest.

Claims (10)

delete delete delete delete A composition for tightening tight junctions in the skin comprising Nelumbo Nucifera and Magnolia officinalis extract mixture as active ingredients, wherein said starch callus and starch extract mixture is present in a dry weight ratio of from 0.1: 1 to 10 : 1. &Lt; / RTI &gt;
delete 6. The method of claim 5,
Wherein the starch callus and starch extract mixture is contained in an amount of 0.001 to 30% by dry weight based on the weight of the total composition.
6. The method of claim 5,
Wherein the composition has the effect of promoting the synthesis of Claudin-1 and Claudin-4 of tight junctions.
6. The method of claim 5,
Wherein the tight junction in the skin epidermis is for enhancing skin barrier function.
9. An external preparation for tightening tight junctions in skin epidermis comprising the composition of any one of claims 5 and 7 to 9.

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