KR102289346B1 - Preparation method of infused oil for skin soothing and atopy improvement - Google Patents

Abstract

The present invention relates to a method for producing an infused oil that effectively shortens the extraction period, and to a method for producing an infused oil having a use for soothing skin or improving atopy by using the same. The infused oil manufacturing method of the present invention can extract the active ingredient within a short time without destroying the active ingredient of the medicinal plant by going through specific sequential steps using lecithin, an emulsifier. In addition, in the case of the infused oil prepared by the method for producing infused oil for skin soothing or atopic improvement of the present invention, it is possible to reduce transepidermal moisture loss due to skin barrier damage and effectively reduce IgE concentration in the blood. It can be usefully used as a bar, functional cosmetic material or pharmaceutical material.

Description

Manufacturing method of infused oil for skin soothing and atopy improvement {Preparation method of infused oil for skin soothing and atopy improvement}

The present invention relates to a method for producing an infused oil that effectively shortens the extraction period, and to a method for producing an infused oil having a use for soothing skin or improving atopy by using the same.

The skin of the human body is an essential organ for maintaining life that protects the body physically and chemically from the outside world and at the same time performs biochemical functions necessary for the metabolism of the whole body. All abnormal findings appearing on the human skin are called skin diseases, that is, skin diseases. Since the skin covers the surface of the body, there are many opportunities for direct contact with stimuli from the outside or various pathogens, and it is strongly influenced by the body. Moreover, even slight changes in the skin can be seen and touched with the naked eye, and it is easy to collect a part of the lesion and examine it histopathologically, or it is easy to perform an examination such as searching for microorganisms, and it is easy to clarify the diagnosis of each disease. , its types reach a significant number compared to other organs, and the disease names are complex and diverse. Relatively common skin diseases include atopic dermatitis, contact dermatitis, seborrheic dermatitis, urticaria, athlete's foot, jock itch, psoriasis, herpes simplex, herpes zoster, dry skin, housewife eczema, and acne.

Atopic dermatitis (AD), one of the skin diseases, is caused by multifactor factors such as immunological, genetic, pharmacological, physiological, and environmental factors. Changes, irritations and allergens are the main causes. Atopic dermatitis is a chronic inflammatory skin disease that is widely induced from infants to adults.

Although the etiology of atopic dermatitis has not yet been clarified, studies to date have shown that T lymphocyte dysfunction is caused by a hypersensitivity response to an increase in immunoglobulin E (IgE) antibodies or a decrease in cell-mediated immune function. Functional deficiency due to fissure differentiation, blockade of adrenergic receptors present in the skin, etc. are presumed to be the cause of the invention.

For the treatment and management of atopic dermatitis, it is common to simultaneously prescribe a steroid hormone, that is, a topical corticosteroid preparation, in order to improve the inflammatory reaction together with a moisturizer that maintains moisture on the skin surface during most dermatological treatments. However, when topical corticosteroids are used for a long period of time, there is a problem in that they cause various skin side effects, such as skin atrophy, vasodilation, loss of pigment and generation of dilated striatum.

Accordingly, many attempts are being made to find a therapeutic agent with high therapeutic effect and fewer side effects from natural products with proven safety. Natural plants and herbal medicines are mainly known as anti-atopic-related substances.

On the other hand, infused oil (Infused oil) refers to an oil obtained by extracting using oil as an extraction solvent when extracting the oil component of a plant. These infused oils have a synergistic effect with the functional ingredients of the oil used as a solvent to extract the oil components of the plant and the active ingredients of the oil extracted from the plant, so that the content of the active ingredient is increased and the functionality is improved. It has the advantage of being applicable to various products.

However, the conventional manufacturing process of infused oil has a disadvantage in that it takes a long extraction period to dissolve the active ingredient in the oil, and a process of applying heat to shorten the extraction period may be performed, but this process There is a problem that destroys the activity of

Therefore, the present inventors studied to find a process that can extract active ingredients in a short time without destroying the active ingredients of medicinal plants, and as a result, infused oil was prepared through specific sequential steps using lecithin, an emulsifier. In this case, the present invention was completed by confirming that the active ingredient could be sufficiently dissolved in the oil even in a very short extraction period of 1 to 6 days without a process of applying heat to the oil.

Korean Patent Registration No. 10-1896908 Korean Patent Registration No. 10-1943346

Accordingly, it is an object of the present invention to provide a method for producing infused oil that can effectively shorten the extraction period.

Another object of the present invention is to provide a method for preparing an infused oil having excellent skin soothing or atopic improving activity using the above method.

In order to achieve the object of the present invention as described above,

The present invention comprises the steps of: a) preparing a first mixture by mixing lecithin and 1,2-hexanediol; b) preparing a second mixture by adding an aqueous component to the first mixture and mixing; c) preparing a tertiary mixture while stirring at room temperature after adding a medicinal plant extract to the secondary mixture; d) preparing a quaternary mixture while stirring after adding oil to the tertiary mixture; And e) provides a method for producing infused oil, comprising the step of aging the quaternary mixture for 1 to 6 days.

In one embodiment of the present invention, the lecithin may be mixed in 5 to 10% by weight.

In one embodiment of the present invention, the aqueous (aqueous) component may be added in an amount of 5 to 15% by weight.

of the present invention In one embodiment, the medicinal plant extract may be added in an amount of 1 to 5% by weight.

In one embodiment of the present invention, the oil may be added in an amount of 70 to 80% by weight.

In one embodiment of the present invention, the method for producing the infused oil can shorten the extraction period.

In addition, the present invention comprises the steps of i) preparing a first mixture by mixing lecithin and 1,2-hexanediol; ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and mixing; iii) preparing a tertiary mixture while stirring at room temperature after adding a medicinal plant extract to the secondary mixture; iv) preparing a quaternary mixture while stirring after adding oil to the tertiary mixture; and v) aging the quaternary mixture for 1 to 6 days.

In one embodiment of the present invention, the medicinal plant extract may be composed of a medicinal plant extract, a malt extract, an extract of elm elm, a pre-clinic extract, a plant extract, a plant extract, a purslane extract and propolis.

In one embodiment of the present invention, the oil may be made of camellia oil, jojoba oil and olive oil.

In addition, the present invention provides an infused oil for skin soothing or atopic improvement prepared by the above method.

The infused oil manufacturing method of the present invention can extract the active ingredient within a short time without destroying the active ingredient of the medicinal plant by going through specific sequential steps using lecithin, an emulsifier. In addition, in the case of the infused oil prepared by the method for producing infused oil for skin soothing or atopic improvement of the present invention, it is possible to reduce transepidermal moisture loss due to skin barrier damage and effectively reduce IgE concentration in the blood. It can be usefully used as a bar, functional cosmetic material or pharmaceutical material.

1 schematically shows the manufacturing process of the infused oil for skin soothing or atopic improvement of the present invention.
2 is a graph showing the measurement of transepidermal moisture loss according to the treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> in the atopic dermatitis animal model (Normal). : normal mouse, Vehicle: negative control, BM: positive control).
3 is a graph showing the measurement of the skin thickness according to the treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> of the present invention in an atopic dermatitis animal model (Normal: normal) Mouse, Vehicle: negative control, BM: positive control).
4 is a graph showing the measurement of blood IgE concentration according to the treatment (application) of the infused oil of <Example 1> or the infused oil of <Comparative Example 1> of the present invention in an atopic dermatitis animal model (Normal: Normal mice, Vehicle: negative control, BM: positive control).

The present invention comprises the steps of: a) preparing a first mixture by mixing lecithin and 1,2-hexanediol; b) preparing a second mixture by adding an aqueous component to the first mixture and mixing; c) preparing a tertiary mixture while stirring at room temperature after adding a medicinal plant extract to the secondary mixture; d) preparing a quaternary mixture while stirring after adding oil to the tertiary mixture; and e) aging the quaternary mixture for 1 to 6 days, characterized in that it provides a method for producing infused oil.

'Infused oil' as referred to in this specification is O'Neal used by immersing herbs or plant flowers or roots in vegetable oil to heat it, or soaking it in sunlight for several weeks and hanging the last residue. , that is, the brewed oil. Since these infused oils have a very high vitamin content extracted from natural plants, they supply abundant moisture and nutrients to the skin to make smooth skin, and natural pigments come out without adding additional pigments. As for the infused oil, Saint John's wort (St. John's grass) and calendula are well known.

The conventional manufacturing process of infused oil has the disadvantage that it takes a long extraction period to dissolve the active ingredient in the oil, and a process of applying heat to shorten the extraction period may be performed, but this process does not There has been a problem of destroying

In the present invention, it has been devised to solve the conventional problems as described above, and by sequentially performing steps a) to e), the active ingredient is extracted within a short time without destroying the active ingredient of the medicinal plant. .

Step a) of the present invention is a step of preparing a primary mixture by mixing an emulsifier and a preservative, in detail, a water-in-oil type (W/O type) emulsifier capable of forming a water-oil (WO) interface. This is a step of preparing a first mixture by mixing phosphorus lecithin and 1,2-hexanediol, which is a preservative.

In one embodiment of the present invention, the lecithin may be mixed in 5 to 10% by weight, preferably 6 to 8% by weight.

Step b) of the present invention is a step of adding and mixing an aqueous component to the lecithin emulsifier composition. Specifically, after adding the aqueous component to the first mixture prepared in step a), the second step is mixed. This step is to prepare a mixture.

As used herein, the term “aqueous component” refers to a component having the properties of water.

In one embodiment of the present invention, the aqueous component may be added in an amount of 5 to 15% by weight.

Step c) of the present invention is a step of adding and mixing a medicinal plant extract, specifically, adding a medicinal plant extract to the second mixture prepared in step b) and then preparing a tertiary mixture while stirring at room temperature am.

As used herein, the term 'medicinal plant' refers to an herb or plant capable of imparting pharmacological action in infused oil.

In one embodiment of the present invention, the medicinal plant extract may be added in an amount of 1 to 5% by weight.

Step d) of the present invention is a step of adding and mixing oil, in detail, adding oil to the tertiary mixture prepared in step c) and then preparing a quaternary mixture while stirring.

As used herein, the term 'oil' refers to a solvent for brewing active ingredients, which is generally vegetable and has a long storage period and is stable. For example, olive oil, macadamia oil, sunflower seed oil, saffflower oil, sweet almond oil, peanut oil, safflower seed oil, camellia oil, jojoba oil and the like may be exemplified, but the present invention is not particularly limited thereto.

In one embodiment of the present invention, the oil may be added in an amount of 70 to 80% by weight.

The method for producing infused oil including steps a) to e) of the present invention can effectively shorten the extraction period compared to the prior art.

In addition, the present invention comprises the steps of i) preparing a first mixture by mixing lecithin and 1,2-hexanediol; ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and mixing; iii) preparing a tertiary mixture while stirring at room temperature after adding a medicinal plant extract to the secondary mixture; iv) preparing a quaternary mixture while stirring after adding oil to the tertiary mixture; and v) aging the quaternary mixture for 1 to 6 days (stored at room temperature).

In one embodiment of the present invention, the medicinal plant extract may be composed of a medicinal plant extract, a malt extract, an elm bark extract, a pre-clinic extract, a plantain leaf extract, a dandelion extract, a purslane extract and propolis.

In another embodiment of the present invention, the oil may consist of camellia oil, jojoba oil and olive oil.

In addition, the present invention provides an infused oil for skin soothing or atopic improvement prepared by the above method.

In addition, the present invention provides a functional cosmetic composition comprising the infused oil for skin soothing or atopic improvement as an active ingredient.

The infused oil of the present invention may be included in an amount of 0.00001 to 20.0% by weight based on the total weight of the cosmetic composition.

When the content of the infused oil is less than 0.00001% by weight, it is not preferable because the original desired skin soothing or atopic improvement effect cannot be sufficiently achieved. may occur.

The cosmetic composition of the present invention contains a moisturizing agent, a thickener, a surfactant, an oily agent, a preservative, an antioxidant, an alcohol, a fragrance, a pH adjuster, a natural extract, etc., as well as a skin A suitable amount of a chemical component to double the sedative or atopic improvement effect may be formulated.

On the other hand, the cosmetic composition prepared by containing the infused oil can be prepared in various formulations such as lotions, essences, skins, lotions, creams, shampoos, and specifically, hair conditioner, hair tonic, It may be prepared in the form of a hair cream, hair lotion, hair oil, hair spray, shampoo or rinse, and may be prepared using a conventional cosmetic preparation method.

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin soothing or atopic dermatitis comprising the infused oil as an active ingredient.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

< Example 1>

of the present invention skin soothing and to improve atopy Infused oil manufacturing

<1-1> Material preparation

The place of purchase of the material used for manufacturing the infused oil of the present invention is as follows.

Ingredients used in the manufacture of infused oil and where to buy them Ingredients Where to buy lecithin Amikos Co., Ltd. 1,2-Hexanethiol Amikos Co., Ltd. chamomile flower water Amikos Co., Ltd. Aloe Vera Leaf Juice Amikos Co., Ltd. Yamba Wheat Extract Amikos Co., Ltd. malt extract Amikos Co., Ltd. Elm Bark Extract Amikos Co., Ltd. Preclipper Extract Amikos Co., Ltd. Parsley leaf extract Amikos Co., Ltd. dapsari extract Amikos Co., Ltd. Purslane Extract Amikos Co., Ltd. propolis extract Amikos Co., Ltd. camellia oil Amikos Co., Ltd. jojoba oil Amikos Co., Ltd. olive oil Amikos Co., Ltd.

<1-2> of the present invention Infused oil manufacturing

To prepare the infused oil of the present invention, first, lecithin (7% by weight) + 1,2-hexanediol (2.7% by weight) were mixed. Thereafter, chamomile flower water (6.9% by weight) and aloe vera leaf juice (6.1% by weight) were added to the mixture and mixed, and then 8 kinds of medicinal plant extract powder (2.4% by weight = about buckwheat extract, malt extract, sugar elm bark) was added and mixed. extract, precliffer extract, plant extract, jasmine extract, purslane extract and propolis extract) were added and the vegetable extract powder was dissolved while stirring at 5000 rpm at room temperature. Thereafter, camellia oil (37% by weight), jojoba oil (10% by weight), and olive oil (27.9% by weight) were added and stirred again at room temperature at 5000rpm. Finally, the infused oil of the present invention was prepared by sealing the raw material and aging for one week. For reference, the 8 kinds of medicinal plant extract powders were mixed and used in the same ratio of each.

< comparative example 1>

through conventional methods Infused oil manufacturing

Camellia oil (40% by weight), jojoba oil (15% by weight) and olive oil (30% by weight) in 10 medicinal plant powders (5% by weight = yam buckwheat extract, malt extract, sugar elm bark extract, preclipper extract , Changkyi leaf extract, dandelion extract, purslane extract, and propolis extract) were added and mixed to prepare an infused oil while stirring at a temperature of 32 to 38° C. for 3 to 5 weeks. For reference, the 10 medicinal plant extract powders were mixed and used in the same ratio of each.

< Experimental example 1>

of the present invention Infused Inhibitory effect of oil on atopic dermatitis

<1-1> Atopic dermatitis animal model production and Infused oil treatment

In order to examine the inhibitory effect of the infused oil of the present invention on atopic dermatitis, oxazolone, a hapten-forming material that artificially induces dermatitis, was administered to BALB/c mice (Orient Bio Inc., Seongnam, Republic of Korea). ) to make atopic dermatitis-like mouse model by applying it to the back, and then the effect of the treatment of the infused oil of the present invention on the symptoms of atopic dermatitis was examined.

First, clean the back of the mouse, and then apply a 10, 0.3% concentration of oxazolone solution (10% and 0.3% oxazolone in ethanol solvent) to the back of the mouse repeatedly for 16 days to induce atopic dermatitis. From the 7th to the 16th day of application, 50 μl each of the infused oil of the present invention prepared in <Example 1> or the infused oil prepared in <Comparative Example 1> was administered twice daily from the 7th to the 16th day of application. applied to the skin. Meanwhile, as a positive control, betamethasone, which has the highest anti-inflammatory action among steroids, was used at a concentration of 0.01%. When 24 hours had elapsed after the last treatment, mice were sacrificed and used in the following experiment.

<1-2> According to skin barrier damage transepidermal water loss measurement

In this experiment, TEWL was measured using a vapometer (Delfin®), and the amount of water lost per unit area and per hour (g/m ² h) was measured as a unit.

As a result, as shown in FIG. 2 , it was confirmed that the degree of transepidermal water loss was reduced when the infused oil of the present invention was treated (applied) to mice induced by atopic dermatitis. The above results indicate that the infused oil treatment (application) of the present invention can suppress the induction of atopic dermatitis symptoms by reducing damage to the skin barrier and effectively reducing skin moisture loss. In particular, it was confirmed that the infused oil of <Example 1> of the present invention can more effectively reduce skin moisture loss compared to the infused oil of <Comparative Example 1>.

<1-3> Measurement of skin thickness

In this experiment, the thickness (mm) was measured using a caliper as a folding method to evaluate the degree of epidermal hyperproliferation as an indicator of the inflammatory response.

As a result, as shown in FIG. 3 , it was confirmed that when the infused oil of the present invention was treated (applied) to a mouse inducing atopic dermatitis, the thickness of the epidermal layer of the mouse was reduced. In particular, when the infused oil of <Comparative Example 1> was treated, the effect of reducing the epidermal layer thickness of the mouse was not large, but when the infused oil of the present invention <Example 1> was treated, the epidermal layer thickness of the mouse was significantly reduced appeared to be

<1-4> blood IgE concentration measurement

At the end of the experiment, blood was collected from the orbital vein of the mouse, and then the serum was separated by centrifugation at 5,000 rpm and 4°C for 3 minutes. It was stored in a -70°C freezer until used in the experiment, then thawed and used for the experiment. The concentration of serum IgE in hairless mice was measured using an ELISA kit for mice (SHIBAYAGI Co., Ltd, Japan), respectively. IgE was quantified according to the method of the ELISA kit for IgE mice (SHIBAYAGI Co., Ltd, Japan). All reagents were used immediately after preparation at room temperature.

Specifically, after washing the plate 3 times with wash buffer, 50 μl of IgE standard solution or sample solution (sample 5 μl + buffer 45 μl) is dispensed into each well, gently mixed with a microplate shaker, and then at room temperature for two hours. After the reaction was incubated at (20-25° C.), the plate was washed 3 times with a buffer solution. 50 μl of biotin-conjugated anti-IgE antibody was dispensed into each well, gently mixed with a microplate shaker, and incubated for two hours at room temperature. After incubation, the plate was washed 3 times with a washing buffer. Then, put 50 μl of HRP-avidin solution into each well, mix lightly with a microplate shaker, and incubate for one hour at room temperature for reaction, add 50 μl of chromagenic substrate solution, and shaker with a microplate. shaker) and incubated for 20 minutes at room temperature for reaction, 50 μl of a reaction stopper was added to each well, mixed well to stop discoloration, and absorbance was measured at 450 nm within 30 minutes.

As a result, as shown in FIG. 4 , when the infused oil of <Example 1> of the present invention was treated (applied) to a mouse inducing atopic dermatitis, the negative control group (vehicle) and the infused oil of <Comparative Example 1> It was confirmed that the concentration of IgE in the blood was significantly reduced compared to the oil-treated group. In particular, compared to the positive control group treated with betamethasone, it was found that the IgE concentration in the blood was further reduced in the experimental group treated with the infused oil of Example 1 of the present invention.

< Experimental example 2>

of the present invention Infused Oil skin irritation test

After absorbing 1ml of the infused oil of the present invention into the gauze part of the band-aid using a band-aid for irritation test, attach it to the upper arm of a person. After 48 hours, the band-aid was removed and the skin was observed after 1 hour. The evaluation method was evaluated according to the evaluation table below.

Judgment was carried out on 50 subjects (100 in total) for each Example, and the results are shown in the table below.

Skin irritation rating table rank state 0 No reaction to the skin at all One Partial skin erythema occurs 2 Overall skin erythema occurs 3 Erythema of the skin occurs and the skin swells 4 Redness of the skin occurs and the skin is severely cracked

Skin irritation degree of the infused oil of the present invention Evaluation items Comparative Example 1 Example 1 skin irritation 0.1 0.1

As shown in Table 3, the infused oil of <Comparative Example 1> and <Example 1> showed almost no skin irritation, confirming that it was stable to the skin when used.

< Experimental example 3>

of the present invention Infused of oil moisturizing power , wound healing effect, itch relief effect, skin strengthening and atopic skin transpiration alleviation effect experiment

Regarding the moisturizing power, wound healing effect, itching symptom alleviation effect, skin strengthening, and atopic skin symptom relief of the infused oil of the present invention, 50 patients with atopic dermatitis were divided into two groups by 25, and administered twice in the morning and in the evening. The effect was evaluated by allowing it to be used on the area showing symptoms of atopic dermatitis.

At this time, the evaluation was performed according to the criteria of very good 10 points, excellent 8 points, average 6 points, bad 4 points, and very bad 2 points, and the results are shown in Table 4.

Evaluation of moisturizing power, wound healing effect, itch relief effect, skin strengthening and atopic skin transpiration alleviation effect of the infused oil of the present invention Evaluation items Comparative Example 1 Example 1 moisturizing power 8.6 9.0 wound healing 7.0 9.2 relieve itching 5.0 9.2 skin strengthening 7.0 8.8 Relief of atopic skin symptoms 5.0 9.4

As shown in Table 4, in the case of the infused oil of <Example 1> of the present invention, compared to <Comparative Example 1>, moisturizing power, wound healing effect, itching alleviation effect, skin strengthening and atopic skin symptom alleviation effect were improved. It was confirmed that it was excellent.

So far, the present invention has been looked at with respect to preferred embodiments thereof. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in modified forms without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (10)

delete delete delete delete delete delete i) preparing a first mixture by mixing lecithin and 1,2-hexanediol;
ii) preparing a second mixture by adding chamomile flower water and aloe vera leaf juice to the first mixture and then mixing;
iii) preparing a tertiary mixture while stirring at room temperature after adding a medicinal plant extract to the secondary mixture;
iv) preparing a quaternary mixture while stirring after adding oil to the tertiary mixture; and
v) A method for producing infused oil for skin soothing or atopic improvement, comprising the step of aging the quaternary mixture for 1 to 6 days. 8. The method of claim 7,
The method according to claim 1, wherein the medicinal plant extract is composed of a medicinal plant extract, malt extract, elm bark extract, pre-clipper extract, spearmint leaf extract, dandelion extract, purslane extract and propolis. 8. The method of claim 7,
The oil is a method, characterized in that consisting of camellia oil, jojoba oil and olive oil. The infused oil for skin soothing or atopic improvement prepared by the method of any one of claims 7 to 9.

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