KR102326660B1 - Compositions for preventing hair loss, promoting hair growth and increasing hair thickness comprising herbal extracts - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss, promoting hair growth, and increasing the hair thickness comprising herbal medicinal extracts. A composition for preventing hair loss, promoting hair growth, and increasing the hair thickness comprising herbal medicinal extracts of the present invention, which is characterized by comprising, as active ingredients, herbal medicinal complex extracts including Houttuynia cordata, Perilla ocymoides, green tea, Glycyrrhiza uralensis, Portulaca oleracea, Eclipta prostrate, Platycladus orientalis, Saururus chinensis, leaves of guava, Fallopia multiflora, Ligustrum japonicum, black soybeans, leaves of Acorus, and Lycium chinense, soothes and softens the scalp and relieves skin inflammation, thereby improving scalp health, increases the hair thickness by strengthening the hair root, promotes hair growth and prevents hairs loss. In addition, the composition including the herbal medicinal extracts of the present invention has an excellent anti-dandruff and anti-itch effect, a hair moisturizing effect and an anti-hair breakage effect. Accordingly, the composition of the present invention has excellent effects of: preventing hair loss; promoting hair growth; increasing the hair thickness; and being harmless or non-toxic to the human body, and thus can be usefully used as a cosmetics and pharmaceutical composition for preventing hair loss, promoting hair growth and increasing the hair thickness.

Description

Composition for preventing hair loss, promoting hair growth and increasing hair thickness, including herbal herbal extracts {Compositions for preventing hair loss, promoting hair growth and increasing hair thickness comprising herbal extracts}

The present invention relates to a composition for preventing hair loss, promoting hair growth and increasing hair thickness, comprising an oriental herbal extract, and more particularly, Eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, It is a composition containing as an active ingredient a complex extract of oriental herbs consisting of Gwangnam, black bean, calamus leaf, and goji berry. It soothes and softens the scalp and relieves skin inflammation to improve scalp health, and increases hair thickness through strengthening the hair root. , It relates to a cosmetic or pharmaceutical composition for hair care for preventing hair loss, promoting hair growth and increasing hair thickness, which can promote hair growth and prevent hair loss as well as have no side effects or toxicity to the human body.

Although there are about 80 to 90,000 hairs on the human scalp, it is normal for ordinary people to lose 50 to 70 hairs every day, but if you lose about 100 or more hairs excessively without any particular reason after washing your hair or going to bed, it is alopecia. can be suspected of The cause of this hair loss is not yet known precisely, but in general, genetic factors, hormonal imbalance, excessive stress, seborrheic dermatitis, poor blood circulation in the scalp (scalp muscle tension), eating habits, nutritional and iron deficiency, hair pulling (traction alopecia), atopic constitution, hair pollution (frequent perm, dyeing, hair dryer use, etc.), air pollution, infectious and inflammatory diseases, chemical factors (alcohol, drugs, smoking), childbirth, menopausal disorders, etc. is known

In the human body, hair grows from hair follicles in the skin, and hair follicles from which hair grows are complex organs composed of inner root epithelium, outer root epithelial sheath, hair stem, and hair matrix. It is characterized by continuous repetition. In particular, among the cells forming the hair follicle, dermal papilla cells play an important role in hair growth, and during the hair follicle growth phase, the growth of hair follicle constituent cells including keratinocytes of the matrix surrounding the dermal papilla occurs. In the degenerative phase of the hair follicle, hair follicle growth is stopped, and apoptosis, condensation of the dermal papilla, and the reorganization of the extracellular matrix occur. this will remain

Hair is divided into a hair root and a hair shaft, and hair grows from the hair follicle surrounding the hair root. Two important factors regulating the biological properties of hair follicles are the lateral root sheath cells (ORS), which are the hair follicle epithelium, and the mesenchymal-derived dermal papilla (DP), and hair grows and falls out through repetition of the hair cycle. Hair goes through three stages: anagen, catagen, and telogen. In the stratum corneum, hair is made and removed through the proliferation and differentiation of the epidermis and hair follicle, respectively. It is known that mesenchymal-derived metastasis amplification cells exist in the follicle, and play a role in maintaining the dermal papilla of hair follicles during growth. Various hormones (testosterone), cytokines (cytokines) and growth factors (growth factors) are known to be involved in hair morphogenesis and hair growth regulation. An increase in the number and size of hair follicles was observed during the growth phase. Unlike the resting phase, which is located in the dermis, during the growth phase, the hair follicles are located in the deep subcutis, so the increase in the number and size of hair follicles is closely related to the growth phase are closely related

Since hair plays an important role in improving external image as a part of appearance, the importance of research on hair growth, wool and hair loss prevention is increasing recently. In particular, as life patterns become westernized, the quality of life improves and stress increases, concerns about hair loss and scalp diseases are expanding to everyone's concerns, and there are simple genetic factors and acquired factors. The importance and interest are gradually increasing. Since hair loss can cause psychological atrophy, etc., which can lead to interpersonal phobia and loss of self-confidence, it can have a great impact on maintaining an individual's rich life, so the market for hair loss industry is continuously growing rapidly.

Hair loss refers to an increase in abnormally falling hair due to a higher proportion of hair growing in the follicles in the degenerative or resting phase than the hair growing in the follicles in the growth phase during the hair follicle growth cycle. In general, it is known to be caused by an unbalanced diet, excessive drinking and smoking, drug use, lack of sleep, excessive stress, childbirth, menopausal disorders, frequent perms and dyeing, and hormonal imbalance. Hair growing on human hair maintains a certain density as the action of birth, growth and fallout is repeated, but when hair loss progresses due to the above various causes, the hair on the crown or forehead first becomes thin and the power begins to disappear. number will increase.

In other words, the hair is rooted in the scalp and supplied with nutrients from the blood. Over a long period of time, when capillaries are reduced and the scalp becomes thin and hair cannot grow normally, the hair in that area becomes thinner and the hair in that area becomes thinner. This becomes shorter and the shorter lifespan will not only fall out easily, but the hair will not grow again in the pores where the hair is lost as described above, which will eventually show the symptoms of empty hair.

With the recent trend to emphasize appearance, interest in hair loss is also increasing. As the cause of hair loss, there have been discussions about poor blood circulation, excessive male hormone action, excessive sebum secretion, hypothalamus and decreased scalp function due to peroxide and bacteria, genetic factors, aging, stress, etc. The cause is unknown. In general, the most typical form of hair loss is androgenetic alopecia, which occurs due to excessive androgen action, and it occurs dependent on androgen, which is a male hormone. is known Testosterone is converted to DHT by 5 alpha-reductase (5-reductase), and this conversion occurs by 5 alpha-reductase of Type 2 among two types of 5 alpha-reductase, Type 1 and Type 2, It has been reported that the gradual reduction of the dermal papilla and hair follicles is progressed by the converted DHT.

Currently, as a representative hair loss treatment agent approved by the US Food and Drug Administration (FDA), there are minoxidil for application and Propecia for oral use as synthetic drugs, and some side effects are continuously reported. Propecia is effective by inhibiting the conversion of male hormone (testosterone) to dihydrotestosterone (DHT), which is the causative agent of hair loss. Although it was developed as a hair growth agent as it has been reported to promote hair growth through

Natural products useful for hair growth studied so far include soybean (Glycine linne, isoflavone), ginger (Zingiber officinale, 6-gingerol), red pepper (Capsicum annuum), aloe (Aloe arborescens), licorice (Glycyrrhiza glabra), and tea tree (Camellia sinensis). ), ginkgo (Ginkgo biloba), avocado (Persea americana), and white hawthorn (Polygonum multiflorum) have been reported.

In addition, studies based on natural extracts were also conducted. For example, extracts (Patent No. 2012-0015874, Patent No. 2010) such as cheongung (Patent No. 2010-0119761), Sanghwang mushroom (Patent No. 2012-0038426), gold and silver flower (Patent No. 2012-0038426), chemomile, etc. -0091571), Hasuo (Patent No. 2013-0021464, Patent No. 2011-0097386), Calamus (Patent No. 2012-0115980), Pomegranate (Patent No. 2012-7012465), Ginkgo biloba extract (Patent No. 2006-7008793) h), and so on. However, when these hair growth agents or hair restorers are actually used in clinical practice, the effect on the scalp is not so excellent. Exploratory research is being actively conducted.

However, despite efforts to utilize such natural products, commercialization of hair growth promoters or hair loss inhibitors is still minimal due to low activity compared to pharmaceuticals.

Accordingly, the present inventors continued research to develop an effective naturally-derived hair growth agent for preventing hair loss and promoting hair growth, and as a result, Eoseongcho, perilla leaf, green tea, licorice, Machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, suo, gwangnamu, A composition containing a complex extract of oriental herbs consisting of black beans, calamus leaves and goji berries soothes and softens the scalp and relieves skin inflammation to improve scalp health, increase hair thickness by strengthening hair roots, promote hair growth and hair loss The present invention was completed by discovering the fact that it is possible to prevent dandruff and has excellent antibacterial and anti-inflammatory effects to prevent dandruff and itching.

Therefore, the technical problem to be solved in the present invention is to improve scalp health by soothing and softening the scalp and alleviating skin inflammation, increasing hair thickness through strengthening the hair root, promoting hair growth and preventing hair loss, as well as To provide a hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness, which has excellent antibacterial and anti-inflammatory effects that prevent dandruff and itching.

In order to solve the above technical problem, in the present invention, an oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf and goji berry. It provides a hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness, characterized in that it contains as an active ingredient.

Preferably, the oriental herbal complex extract is 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of extract of oleracea based on 100 parts by weight of extract of Eoseongcho. 70 to 80 parts by weight of extract, 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of extract of trifolieae, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of sauerkraut extract, 40 to 60 parts by weight of extract It is characterized in that it consists of 90 to 110 parts by weight of the extract, 70 to 80 parts by weight of the calamus leaf extract, and 20 to 30 parts by weight of the Goji berry extract.

Preferably, the hair care composition is a cosmetic composition.

Preferably, the cosmetic composition may be a hair shampoo, hair conditioner, hair essence, hair nutrition, hair oil, hair gel, hair conditioner, hair treatment or hair dye.

Preferably, the hair care composition is a pharmaceutical composition.

As described above, the composition comprising the oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf, and goji berry of the present invention Soothes and softens, improves scalp health by alleviating skin inflammation, increases hair thickness by strengthening the hair root, promotes hair growth and prevents hair loss. In addition, the composition comprising the oriental herbal complex extract of the present invention is excellent in the anti-dandruff and itching effect, the moisturizing effect of the hair, and the anti-cracking effect. Therefore, the composition of the present invention not only has an excellent effect of preventing hair loss, promoting hair growth and increasing hair thickness, but also has no side effects or toxicity to the human body, so it can be usefully used as a cosmetic and pharmaceutical composition for preventing hair loss, promoting hair growth and increasing hair thickness. is expected to

Hereinafter, the present invention will be described in more detail.

In the present invention, it is characterized in that it contains an oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, succulent, gwangnamu, black bean, calamus leaf and goji berry as an active ingredient. It provides a hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness.

According to one embodiment of the present invention, the oriental herbal complex extract is 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of licorice extract, based on 100 parts by weight of eoseongcho extract. 70 to 80 parts by weight, 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of trifolieae extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of H. to 60 parts by weight, 90 to 110 parts by weight of black bean extract, 70 to 80 parts by weight of iris extract, and 20 to 30 parts by weight of Goji berry extract.

The composition comprising the oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, hawthorn, gwangnamu, black bean, calamus leaf, and goji berry of the present invention soothes and softens the scalp It can improve scalp health by alleviating skin inflammation, increase hair thickness by strengthening the hair root, promote hair growth and prevent hair loss.

The composition comprising the oriental herbal complex extract of the present invention has excellent anti-dandruff and itch-preventing effects, hair moisturizing effects and anti-cracking effects.

As used herein, the term “hair loss” refers to a phenomenon in which hair is completely pulled out of the scalp. In addition, the term "hair growth" in the present specification means that hair grows from the scalp, and the term "hair growth" of the present specification means the length of the hair becomes longer, and has the same meaning as "wool", which is another term used in the art. is used as

The term "prevention" used in the present invention means any action that inhibits or delays the occurrence, spread, and recurrence of hair loss disease by administration of the composition according to the present invention, and the term "treatment" used in the present invention means the present invention By the administration of the composition, the progress of hair loss is delayed or stopped to improve the symptoms of hair loss, or hair growth and hair growth, such as lengthening or increasing the number of hairs, are all acts of beneficially changing the symptoms of hair loss.

Those of ordinary skill in the art to which the present invention pertains, with reference to the data presented by the Korean Medical Association, etc., know the exact criteria of the disease for which the composition of the present invention is effective, and can determine the degree of improvement, improvement and treatment There will be.

Houttuynia cordata Thunberg, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth, and increasing hair thickness of the present invention, refers to the above-ground part of the flowering period of yak buckwheat belonging to the family Siberiaceae. It is a name and the taste is slightly astringent. As a traditional medicinal plant, it is effective in detoxification and diuresis, and it is known to be externally applied to lung abscess, phlegmon, pneumonia, hydrocephalus, beriberi, urinary tract infection, white sores, hyperemia, polyps, and ulcers. It has the effect of reducing fever, detoxification, diuresis, and alleviating swelling. Antibacterial action, immune enhancing action, anti-inflammatory action, diuretic action and antitussive action have been reported as pharmacological actions of Eoseongcho. The main components are flavonoids such as quercetin, quercitrin, isoquercetin, afzerin, rutin, and aristolactam A, AII, B, BII, piperolactam A, norcepharadione B, cepharadione B, splendidine, etc. have been reported as Alkaloids.

Perilla frutescens (L.), an active ingredient of the hair care composition for preventing hair loss, promoting hair growth, and increasing hair thickness of the present invention, is the leaf and tip of chazugi or wrinkled lobule belonging to the Lamiaceae (Labiatae). It is dried and used medicinally. Perilla leaves have traditionally been used to treat chills, fever, cough, asthma, and chest tightness caused by colds, as well as food poisoning caused by fish and crabs. It is known that there is

According to one embodiment of the present invention, the perilla leaf extract is used in an amount of 40 to 60 parts by weight based on 100 parts by weight of the eoseongcho extract. In this case, when the perilla leaf extract is used in an amount of less than 40 parts by weight or in an amount of more than 60 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the desired effect of the present invention.

Green tea, which is an active ingredient of the hair care composition for preventing hair loss, promoting hair growth, and increasing hair thickness of the present invention, is processed from the buds or leaves of Camellia sinensis O Kuuntze belonging to the Camellia family. Green tea contains catechins, caffeine, amino acids, and fiber It contains various ingredients such as , pectin, and vitamins. These ingredients show various effects such as antioxidant action, antibacterial action, anti-inflammatory action, deodorant action, astringent action, caries prevention effect, and anti-aging action against skin damage caused by UV rays. In particular, EGCg, ECg, EGC, etc. catechins have an affinity for proteins, so they have the effect of cleaning the contaminated stratum corneum and enhancing the skin's moisturizing power, inhibiting bacteria, and removing body odors.

According to one embodiment of the present invention, the green tea extract is used in an amount of 40 to 60 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the green tea extract is used in an amount of less than 40 parts by weight or in an amount of more than 60 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Licorice (Glycyrrhiza uralensis Fischer, Glycyrrhiza glabra Linne) which is an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention is a perennial herbaceous plant belonging to the legume family. Pharmacological action is effective for detoxification, hepatitis, urticaria, dermatitis, and eczema. It has anti-inflammatory, expectorant, muscle relaxation, diuretic, and anti-inflammatory effects, and suppresses peptic ulcer.

According to one embodiment of the present invention, the licorice extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the licorice extract is used in an amount of less than 70 parts by weight or in an amount of more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Portulaca oleracea, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth, and increasing hair thickness of the present invention, is also called purslane, Jangmyungchae, gwayeopchae, and five elements. Machihyun has antipyretic, detoxifying and hemostatic effects, and antibacterial effects have also been reported.

According to one embodiment of the present invention, the extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the extract is used in less than 70 parts by weight or in an amount of more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Eclipta prostrats, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, is a plant of the Asteraceae family, and is classified as a wetland plant because it is mainly observed in very humid soil. It is a plant that grows in tropical and subtropical wetlands around the world, including India. The leaves have a slightly spicy and sour taste. In Donguibogam, it is stated that orchids make hair longer and darken white hair. If nutrients are not properly supplied to the scalp due to the heat of the body, the hair loss process is accelerated.

According to one embodiment of the present invention, the orchid extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the Eoseongcho extract. At this time, when the orchid extract is used in an amount of less than 70 parts by weight or in an amount of more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Chinese arbor vitae, which is an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, contains thujene, pinene, and the like among volatile essential oil components. Arborvitae is known to contain a lot of phytoncide, a natural antibacterial component.

According to one embodiment of the present invention, the cypress extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the arborvitae extract is used in an amount of less than 70 parts by weight or in an amount of more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Saururus chinensis, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness, is a perennial herb grown in Jeju Island and some areas of Jirisan Mountain. It is known to have an excellent anti-aging effect by facilitating the flow.

According to one embodiment of the present invention, the extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the extract is used in an amount of less than 70 parts by weight or more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Guava leaf (Psidium guayava LINN.), an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, contains vitamin C and inorganic components such as phosphorus, iron, calcium, potassium and magnesium and tannin components. It contains a large amount of high-functional ingredients, and is known to have preventive and therapeutic effects against gastrointestinal disorders, aging, hypertension, diabetes, and cancer.

According to one embodiment of the present invention, the guava leaf extract is used in an amount of 20 to 30 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the guava leaf extract is used in an amount of less than 20 parts by weight or in an amount of more than 30 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Polygonum Multiflorum root, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth, and increasing hair thickness of the present invention, refers to a drug made from the tuberous root of Polygonum multiflorum Thunberg (Polygonaceae), It is known to darken the skin, prevent aging, activate the brain, replenish blood, and enhance energy.

According to one embodiment of the present invention, the sewage extract is used in an amount of 90 to 110 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the extract is used in less than 90 parts by weight or in an amount of more than 110 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Ligustrum japonicum, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, is an evergreen shrub belonging to the family Oleaceae, and its fruits include oleanicacid, mannitol, glucose, palmitic acid, and stearic acid. , oleic acid, and linolenic acid were contained. The peel contained oleanolic acid, acetyl oleanolic acid, and ursolic acid, and the seeds contained 14.9% of fat oil. Among fatty oils, palmitic acid and stearic acid are 19.5%, oleic acid, linoleic acid, etc. 80.5%. Oleanolic acid has mild cardiac and diuretic action, and mannitol has a relieving action and contains a large amount of glucose. Gwangnam tree is used for ornamental and medicinal purposes, and in oriental medicine and folk medicine, the fruits are used for tonic, boil, medicinal spirit, boik, beriberi, and punghyeol.

According to one embodiment of the present invention, the Gwangnam extract is used in an amount of 40 to 60 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the Kwangnam extract is used in an amount of less than 40 parts by weight or in an amount of more than 60 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

Black beans, an active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, are known to relieve the poison of all drugs by controlling the kidneys, eliminating edema, and facilitating blood circulation. It contains a component (cysteine) that promotes growth and is effective in preventing hair loss.

According to one embodiment of the present invention, the black bean extract is used in an amount of 90 to 110 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the black bean extract is used in an amount of less than 90 parts by weight or in an amount of more than 110 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

The active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, iris, is rich in tannins and vitamins, and contains a large amount of aromatic essential oil. Therefore, it is effective in calming the scalp and hair damaged by UV rays or other chemicals, emitting the characteristic scent of iris, and keeping the scalp clean.

According to one embodiment of the present invention, the calamus leaf extract is used in an amount of 70 to 80 parts by weight based on 100 parts by weight of the eoseongcho extract. At this time, when the iris leaf extract is used in an amount of less than 70 parts by weight or in an amount of more than 80 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

The active ingredient of the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention is Goji berry (Lycii fructus). . According to Donguibogam, goji berries are effective for nourishment, tonicity, blood, and jigal. It is recorded that it is good for diseases that paralyze the nerves and improves the eyesight by promoting the functions of the lungs and kidneys. Goji berry is effective for chronic hepatitis. When taken for liver cirrhosis, etc., inflammation is removed and functions are activated.

According to one embodiment of the present invention, the Goji berry extract is used in an amount of 20 to 30 parts by weight based on 100 parts by weight of the Eoseongcho extract. At this time, when the Goji berry extract is used in an amount of less than 20 parts by weight or in an amount of more than 30 parts by weight, it is difficult to obtain the effect of preventing hair loss, promoting hair growth and increasing hair thickness, which is the purpose of the present invention.

As used herein, the term “extract” refers to a preparation obtained by squeezing oriental herbal herbs with an appropriate leaching solution and evaporating the leachate, and is not limited thereto, but is not limited thereto, but is an extract obtained by extraction, a diluted or concentrated solution of the extract, or an extract. It may be a dried product obtained by drying, a crude product or a purified product thereof.

In the present invention, the extract of each herbal herb leaf, young shoot, main body, main body shell, stem, stem peel, root, root shell, rhizome, seed, fruit, immature, mature, fruit, pericarp, flower, It can be obtained from any one selected from the group consisting of androecium, gynoecium, calyx, stamens, petals, calyx fragments, carpels, and combinations thereof.

The oriental herbal extract of the present invention can be prepared using general extraction methods, separation and purification methods known in the art. As the extraction method, a method such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used, but is not limited thereto.

Specifically, oriental herbal extracts are used by pulverizing oriental medicinal herbs as they are, or after drying and pulverizing using a hot air dryer or freeze dryer, an extraction solvent, such as water, anhydrous or hydrous lower alcohols having 1 to 3 carbon atoms, and mixed solvents thereof The solvent selected from the group consisting of may be extracted using an extraction solvent, preferably water or ethanol, and the amount of the extraction solvent may be 2 to 20 times by weight, preferably 5 to 15 times by weight of the dry weight of the natural plant. have.

According to the present invention, extract and mix eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf and goji berry, respectively, or mixed or eoseongcho, perilla leaf, green tea, Licorice, machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf and goji berry may be mixed and then extracted, but there is no particular limitation.

That is, according to one specific embodiment of the present invention, eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, suo, gwangnamu, black bean, calamus leaf, and goji berry about 1 of each sample weight Alcohol having 1 to 5 carbon atoms, including water, methanol, ethanol, propanol, isopropanol, butanol, etc. Or a mixed solvent of water and methanol having a mixing ratio (kg/l) of about 1:0.1 to 1:10, preferably 1:0.2 to 1:9, for about 2 to 7 days, preferably 3 to 5 days. Using extraction methods such as stirring extraction, hot water extraction, chilled extraction, reflux cooling extraction, or ultrasonic extraction, preferably, the extract obtained after ultrasonic extraction is filtered, concentrated under reduced pressure, or dried, Extracts, licorice extract, horseradish extract, chrysanthemum extract, chrysanthemum extract, trifolieae extract, guava leaf extract, hawthorn extract, gwang tree extract, black bean extract, calamus leaf extract and goji berry extract are obtained and then mixed to prepare a herbal complex extract. can

In addition, according to one specific embodiment of the present invention, eoseongcho, perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, suo, gwangnamu, black bean, calamus leaf and goji berry are mixed in advance, and then the sample Carbon number including water, methanol, ethanol, propanol, isopropanol, butanol, etc. in an amount of about 1 to 40 times, preferably about 10 to 20 times the total weight A mixed solvent of 1 to 5 alcohol or water and methanol having a mixing ratio (kg/l) of about 1:0.1 to 1:10, preferably 1:0.2 to 1:9, for about 2 to 7 days, preferably is extracted using an extraction method such as stirring extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction for 3 to 5 days, preferably after ultrasonic extraction, filtering, concentrating or drying the obtained extract under reduced pressure to extract oriental herbal complex extract can be obtained.

The oriental herbal extract may be one in which moisture has been completely removed through concentration and freeze-drying of the obtained extract, and the oriental herbal extract from which the moisture has been completely removed is used in powder form, or the powder is dissolved in distilled water or a common solvent. Can be used.

In the hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention, the complex oriental herbal extract contains 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 60 parts by weight of licorice extract based on 100 parts by weight of eoseongcho extract. 80 parts by weight, 70 to 80 parts by weight of Machishu extract, 70 to 80 parts by weight of orchid extract, 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of extract of chrysanthemum extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of Suo extract It is characterized in that it is mixed in an amount of 40 to 60 parts by weight of Gwangnam extract, 90 to 110 parts by weight of black bean extract, 70 to 80 parts by weight of iris extract, and 20 to 30 parts by weight of Goji extract.

As used herein, the term 'comprising as an active ingredient' means including an amount sufficient to achieve the efficacy or activity of the oriental herbal complex extract. In the present invention, the oriental herbal complex extract may be included in an amount of 1 to 90% by weight, preferably 10 to 80% by weight, more preferably 30 to 60% by weight based on the total weight of the composition.

The hair care composition comprising the herbal complex extract of the present invention as an active ingredient is alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, hereditary hypotrichosis simplex ( hereditary hypotrichosis simplex, circumscribed alopecia, alopecia congenitalis, alopecia pubis, seborrheic alopecia (alopecia seborrheica), alopecia senilis, alopecia totalis, total alopecia (alopecia totalis) alopecia universalis), and telogen effluvium can be used to improve, prevent, and treat alopecia of any one selected from the group consisting of alopecia.

The present invention relates to 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of Machishu extract, 70 to 80 parts by weight of chrysanthemum extract based on 100 parts by weight of extract of Eoseongcho. , 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of trifolieae extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of sauerkraut extract, 40 to 60 parts by weight of Gwangju extract, 90 to 110 parts by weight of black bean extract , It provides a cosmetic composition for preventing hair loss, promoting hair growth and increasing hair thickness, characterized in that it contains an oriental herbal complex extract consisting of 70 to 80 parts by weight of iris extract and 20 to 30 parts by weight of Goji berry extract as an active ingredient.

The cosmetic composition of the present invention can be prepared by including a cosmetically effective amount of the active ingredient of the present invention described above and a cosmetically acceptable carrier.

The appearance of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It is in any dosage form suitable for topical application, for example solutions, gels, solids, kneaded dry products, emulsions obtained by dispersing the oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition is not particularly limited in its formulation, for example, a form selected from the group consisting of tonic, cream, pack, lotion, spray, pomade, powder, gel, sol gel, emulsion, oil, wax and aerosol. It may be a cosmetic for hair or scalp.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

According to one embodiment of the present invention, the cosmetic composition may be a hair shampoo, hair conditioner, hair essence, hair nutrition, hair oil, hair gel, hair conditioner, hair treatment or hair dye.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the active ingredients. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.

In the cosmetic composition of the present invention, a component contained in a general cosmetic composition may be further blended with the functional additive as needed. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

The present invention relates to 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of Machishu extract, 70 to 80 parts by weight of chrysanthemum extract based on 100 parts by weight of extract of Eoseongcho. , 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of trifolieae extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of sauerkraut extract, 40 to 60 parts by weight of Gwangju extract, 90 to 110 parts by weight of black bean extract , It provides a pharmaceutical composition for preventing hair loss, promoting hair growth and increasing hair thickness, characterized in that it contains an oriental herbal complex extract consisting of 70 to 80 parts by weight of iris extract and 20 to 30 parts by weight of Goji berry extract as an active ingredient.

The pharmaceutical composition may include powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations of aerosols, liquid, cream, lotion, paste or solid transdermal topical application formulations (external preparations for skin), suppositories , it may be one or more formulations selected from the group comprising sterile injectable solutions and sprays. Preferably, it may be in the form of an external preparation for skin. The external preparation for skin is a generic term that can include anything applied outside the skin, and various types of pharmaceuticals and cosmetics can be included here.

The therapeutically effective amount of the pharmaceutical composition of the present invention may vary depending on several factors, for example, administration method, target site, patient's condition, and the like. Therefore, when used in the human body, the dosage should be determined as an appropriate amount in consideration of both safety and efficiency. It is also possible to estimate the amount used in humans from the effective amount determined through animal experiments. These considerations in determining effective amounts are found, for example, in Hardman and Limbird, eds., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 10th ed. (2001), Pergamon Press; and E.W. Martin ed., Remington's Pharmaceutical Sciences, 18th ed. (1990), Mack Publishing Co.

The pharmaceutical composition of the present invention may also include a carrier, diluent, excipient, or a combination of two or more commonly used in pharmaceutical formulations. A pharmaceutically acceptable carrier is not particularly limited as long as it is suitable for in vivo delivery of the composition, see, for example, Merck Index, 13th ed., Merck & Co. Inc. Compounds described in , saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components can be mixed and used, and if necessary, other antioxidants, buffers, bacteriostats, etc. Conventional additives may be added. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to formulate into injectable formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules or tablets. Furthermore, it can be preferably formulated according to each disease or component using an appropriate method in the art or a method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA, 18th, 1990).

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable additive, wherein the pharmaceutically acceptable additive includes starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, calcium hydrogen phosphate. , lactose, mannitol, syrup, gum arabic, pregelatinized starch, corn starch, powdered cellulose, hydroxypropyl cellulose, Opadry, sodium starch glycolate, lead carnauba, synthetic aluminum silicate, stearic acid, magnesium stearate, aluminum stearate, Calcium stearate, sucrose, dextrose, sorbitol and talc and the like can be used. The pharmaceutically acceptable additive according to the present invention is preferably included in an amount of 0.1 to 90 parts by weight based on the composition, but is not limited thereto.

The pharmaceutical composition of the present invention may be administered parenterally (for example, intravenously, transdermally, intraperitoneally or topically) or orally according to a desired method, and the dosage may vary depending on the weight, age, sex, health of the patient. The range varies depending on the condition, diet, administration time, administration method, excretion rate, and the severity of the disease.

Liquid formulations for oral administration of the pharmaceutical composition of the present invention include suspensions, internal solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, such as wetting agents, sweeteners, and fragrances , preservatives, etc. may be included together. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, suppositories, and the like.

Since the composition for preventing hair loss, promoting hair growth and increasing hair thickness according to the present invention contains natural oriental herbs as an active ingredient, even when used as a cosmetic composition or pharmaceutical composition, side effects may be less than that of general synthetic compounds, so it is safe to use a cosmetic composition or It can be usefully included in a pharmaceutical composition.

The composition comprising the oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, hawthorn, gwangnamu, black bean, calamus leaf, and goji berry of the present invention soothes and softens the scalp It can improve scalp health by alleviating skin inflammation, increase hair thickness by strengthening the hair root, promote hair growth and prevent hair loss.

In addition, the composition comprising the oriental herbal complex extract of the present invention is excellent in the anti-dandruff and itching effect, the moisturizing effect of the hair, and the anti-cracking effect.

Therefore, the composition of the present invention not only has an excellent effect of preventing hair loss, promoting hair growth and increasing hair thickness, but also has no side effects or toxicity to the human body, so it can be usefully used as a cosmetic and pharmaceutical composition for preventing hair loss, promoting hair growth and increasing hair thickness. is expected to be

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of a composition for preventing hair loss, promoting hair growth, and increasing hair thickness, including herbal herbal complex extract

Eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, sagebrush, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf, and goji berry 1kg each in purified water and heat extraction at 100℃ for 1 hour to obtain each eoseongcho, Perilla leaf, green tea, licorice, machihyun, chrysanthemum, chrysanthemum, trifolieae, guava leaf, sauerkraut, gwangnamu, black bean, calamus leaf and goji berry extracts were obtained. The extract was filtered under reduced pressure with Advantec No. 2 (110mm) filter paper to remove insoluble substances, and then concentrated under reduced pressure at 40° C. using a concentrator equipped with a cooling condenser. In order to completely remove the solvent of the concentrated extract under reduced pressure, 0.5ℓ of purified water was added and suspended, and then each eoseongcho extract, perilla leaf extract, green tea extract, licorice extract, horseradish extract, chrysanthemum extract, chrysanthemum extract, trifolieae extract , guava leaf extract, sauerkraut extract, gwangnam tree extract, black bean extract, calamus leaf extract and goji berry extract were obtained.

The obtained eoseongcho extract, perilla leaf extract, green tea extract, licorice extract, horseradish extract, chrysanthemum extract, chrysanthemum extract, trifolieae extract, guava leaf extract, sauerkraut extract, guava leaf extract, black bean extract, calamus leaf extract and goji berry extract A composition for preventing hair loss, promoting hair growth and increasing hair thickness was prepared by mixing according to the composition ratio (unit g) shown in Table 1.

<Comparative Examples 1 to 10>

A composition was prepared in the same manner as in Example 1 according to the composition ratio shown in Table 1 below.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Eoseongcho Extract 200 200 200 200 200 200 200 200 200 200 200 Perilla leaf extract 100 - - 100 100 100 100 100 100 100 100 green tea extract 100 - 100 - - 100 100 100 100 100 100 Licorice Extract 150 150 - 150 150 - - 150 150 150 150 Machiba extract 150 150 150 - 150 150 150 - - 150 150 orchid extract 150 150 150 150 - 150 150 150 150 - - Arborvitae Extract 150 150 - 150 150 - 150 150 150 150 150 Trifolium Extract 150 150 150 150 150 150 - 150 - 150 - Guava Leaf Extract 50 50 50 - 50 50 50 - 50 50 50 Hawthorn Extract 200 200 200 200 - 200 200 200 200 200 - brine extract 100 100 100 100 100 - 100 100 100 - 100 Black Bean Extract 200 - 200 200 200 200 - 200 200 200 200 Calamus Leaf Extract 150 150 150 150 150 150 150 - 150 150 150 Goji berry extract 50 50 50 50 50 50 50 50 - - 50

<Test Example 1> Hair growth promoting efficacy analysis

The hair growth efficacy through cell proliferation was confirmed in the composition for preventing hair loss, promoting hair growth and increasing hair thickness prepared in Example 1 and the compositions prepared in Comparative Examples 1 to 10.

Human hair follicle dermal papilla cells (breast papilla cells, DP cells) were purchased from Cell Bio, and low glucose DMEM medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin was used as a culture medium at 37°C, CO ₂ incubator. cultured in

The DP cell suspension was added to a 96-well plate at an appropriate concentration and then stabilized in an incubator for 24 hours. Each was treated at an amount of 2 μg/ml and incubated for 48 hours. After 48 hours, the media was removed and washed with PBS. 100 μl of MTT solution dissolved in PBS at a concentration of 1 mg/ml was dispensed and incubated for 4 hours in an incubator at 37°C, then the supernatant was removed, 200 μl DMSO was added to completely dissolve formazan, and then an ELISA plate reader was used. to measure the OD value at 540 nm.

The statistical significance of test results was expressed as mean ± standard deviation, and the difference between each group was analyzed for significance by using the SPSS 25 (SPSS Inc. USA) program, and by performing post-hoc tests using ANOVA and Duncan's test. A case of P<0.05 or less was considered significant. Each experiment was performed at least 3 times and statistically processed, and the proliferation rate (%) of dermal papilla cells is shown in Table 2 below.

dermal papilla cell proliferation rate (%) DMSO 100.00±0.00 minoxidil 105.12±0.21 Example 1 107.23±0.09 Comparative Example 1 101.00±0.12 Comparative Example 2 101.00±0.23 Comparative Example 3 100.24±0.15 Comparative Example 4 100.27±0.24 Comparative Example 5 101.12±0.09 Comparative Example 6 100.54±0.22 Comparative Example 7 100.32±0.28 Comparative Example 8 101.10±0.16 Comparative Example 9 100.28±0.08 Comparative Example 10 100.29±0.32

As shown in Table 2, the minoxidil (MNXD) group, a positive control, increased to 105.12±0.21% compared to the control group, which was 100.00±0.00%, and Comparative Examples 1 to 10 showed a microscopic effect, whereas the practice of the present invention In Example 1, it can be seen that the proliferation rate of dermal papilla cells was significantly increased to 107.23±0.09.

<Test Example 2> Anti-dandruff growth inhibitory ability analysis

A growth inhibition test for Pityrosporium ovale (P. ovale), a dandruff bacterium that exists in hair and scalp, and causes hair loss by growing nutrients of hair follicles as food, was conducted. The strain of ATCC14521 was purchased from the cell line bank and used. Inoculate P. ovale into the prepared dandruff plate medium and incubate for 48 hours at 37°C in 5% CO2. After culturing, P. ovale bacteria are dissolved in a diluted solution using a platinum tooth to make a suspension with a concentration of 108 CFU/ml. The bacterial suspension is evenly inoculated into the dandruff culture medium using a sterile cotton swab, and a sterile disk with a diameter of 8.0 mm is placed on the center of the medium. After dropping 60 μl of a sample diluted to 50% with purified water onto a disk, it was incubated at 37° C. and 5% CO 2 conditions for 48 hours. After measuring the total diameter of the sample and the low zone, the growth inhibition zone (low fat ring) was obtained according to Equation 1 below.

W: Width of growth block (mm)

T: Total diameter of sample and low-lying area (mm)

D: diameter of sample (mm)

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 low-growth
width
(mm) 13.9 8.7 8.3 8.7 8.2 8.6 8.6 8.2 8.5 8.3 8.4

As a result of examining the antifungal effect for Example 1 and Comparative Examples 1 to 10, the width of the growth inhibition zone for P. ovale, a representative dandruff in Example 1, was 13.9 mm, which is significantly higher than that of Comparative Examples 1 to 10. showed effect.

<Test Example 3> Anti-inflammatory effect analysis

In this experiment, the NO production inhibitory activity was measured to investigate the anti-inflammatory activity of the composition for preventing hair loss, promoting hair growth and increasing hair thickness prepared in Example 1. In order to confirm the anti-inflammatory effect of the inhibition of NO production, a nitric oxide (NO) formation inhibitory test was performed by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278). Specifically, RAW264.7 cells, which are macrophages of mice, were subcultured several times, placed ^{in a 48-well plate at 1.0x10 5} cells/well in one well, and cultured for 18 hours. Then, it was replaced with a new medium containing samples prepared by concentration. At this time, L-NMMA (L-NG-Monomethylarginine), which is a NO-production inhibitor, was treated together as a positive control and incubated for 30 minutes. was treated with 1 μg of LPS (Lipopolysaccharide) and cultured for 24 hours. Take 100 μl of the supernatant, transfer it to a 96-well plate, add 100 μl of GRIESS solution, react for 10 minutes at room temperature, measure the absorbance at 540 nm to determine the NO inhibitory effect, and the results are shown in Table 4 below It was. All result values were expressed as mean ± standard deviation (SD) through three or more independent experiments.

NO production rate (%) LPS(-) (control) 10.37 LPS(+) 100 2-Amino-4-methylpyridine
(positive control) 15.86 Example 1 18.45 Comparative Example 1 54.67 Comparative Example 2 52.42 Comparative Example 3 58.23 Comparative Example 4 60.01 Comparative Example 5 59.34 Comparative Example 6 57.25 Comparative Example 7 55.32 Comparative Example 8 56.28 Comparative Example 9 60.71 Comparative Example 10 57.98

As shown in Table 4, it was confirmed that the expression level of cytokines increased by LPS was reduced by the composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention. This can be expected to have a synergistic effect as the anti-inflammatory effect acts as an auxiliary role in calming the scalp.

<Test Example 4> Analysis of hair growth promoting effect

Test Subject Selection Criteria: Men and women aged 18 to 54 years of age diagnosed with androgenetic alopecia. According to Basic and specific (BASP) classification, the basic type is M2 or higher or C2 or higher or U1 or higher, and the specific type is V1 or higher or F1 or higher. The subjects were male and female androgenetic alopecia patients, male patients diagnosed as 3 or higher by the orwood-Hamilton classification, or female patients diagnosed as abnormal by Ludwig classification.

Equipment used: (1) ^{A computer equipped with a Folliscope ⓡ} (a high-resolution video microscopic camera and a separate folliscope software Phototrichogram) is used to analyze the main characteristics of hair, such as density, thickness, growth rate, etc. system equipment), (2) digital camera Canon EOD 750D) (it was attached to a fixed cradle so that the subject's crown and hairline could be photographed at the same angle and position as used when taking clinical pictures).

Test procedure: Two weeks before the start of the test, the subjects were determined, consent wash out products were issued, and the test site was tattooed. At the 10th and 20th weeks of the test, 20 weeks' worth of test samples were issued after visual evaluation by folliscope motion and photography, stability evaluation, and questionnaire evaluation.

Table 5 below shows the average number of hairs after 10 weeks and 20 weeks of the test.

0 parking Week 10 Week 20 Example 1 78.65±12.95 87.34±14.58 98.29±10.65 Comparative Example 1 79.41±10.02 82.56±09.34 83.26±08.58 Comparative Example 2 78.43±11.23 82.51±12.62 84.72±12.47 Comparative Example 3 79.55±12.28 82.67±17.71 85.34±09.28 Comparative Example 4 78.66±11.87 82.81±14.37 83.27±12.36 Comparative Example 5 79.71±16.23 82.31±07.51 84.26±08.47 Comparative Example 6 79.39±10.15 81.43±08.36 83.69±07.32 Comparative Example 7 78.39±12.56 81.28±08.45 83.58±11.35 Comparative Example 8 78.68±12.65 82.75±09.61 84.28±12.56 Comparative Example 9 78.58±13.38 81.28±11.64 83.24±14.27 Comparative Example 10 79.61±17.38 82.69±10.37 84.29±13.17

As shown in Table 5, in the case of the composition for preventing hair loss, promoting hair growth and increasing hair thickness of Example 1, the hair was at a statistically significant level (p<005), such as 78.65 at 0 weeks, 87.34 at 10 weeks, 98.29 at 20 weeks, etc. number increased. On the other hand, in the case of Comparative Examples 1 to 10, a statistically significant level difference (p<005) could not be confirmed at 0, 10, and 20 weeks.

<Test Example 5> Sensory evaluation

The sensory evaluation was conducted for a total of 10 people of each age group from 20s to 40s, once every evening in a conventional manner, using the hair shampoo containing the composition for preventing hair loss, promoting hair growth and increasing hair thickness of Example 1 After 3 months, hair moisturizing effect, scalp softening effect, hair splitting prevention effect, scalp itching prevention effect, and overall preference were tested. For each evaluation item, the highest excellent condition was rated as 10 points, average condition 5 points, and poor condition 0 points. As Comparative Example 11, a commercially available general hair shampoo was used. The results are shown in Table 6 below.

hair moisturizing scalp softening crack prevention anti-itch overall sign Example 1 10 10 10 10 10 Comparative Example 11 6 7 6 3 5

As shown in Table 6, the hair shampoo containing the composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention has a moisturizing effect on the hair, a softening effect on the scalp, an effect on preventing hair splitting and an effect on preventing itching of the scalp and overall effect, compared to general shampoos. It was confirmed that the degree of acceptance was very good.

<Test Example 6> Formulation stability test

Formulation stability test of the composition for preventing hair loss, promoting hair growth and increasing hair thickness prepared in Example 1 was performed.

The observation period was the same for one week, and stability was observed at various temperatures, and the results are shown in Table 7 below.

Temperature 0℃ 40℃ -10℃ Example 1 Good Good Good

As shown in Table 7, it was confirmed that the composition for preventing hair loss, promoting hair growth and increasing hair thickness prepared in Example 1 had good stability at various temperatures.

<Test Example 7> Human safety confirmation experiment

In order to confirm that the composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention is safe for human skin, a skin safety verification experiment was performed. For this purpose, a cumulative skin irritation test was performed. A formulation was prepared with squalene as a base and 1% of the composition for preventing hair loss, promoting hair growth and increasing hair thickness of Example 1 was added, and using this, 20 healthy adults were treated on the upper forearm area for a total of 9 times every other day 24 times. Whether the composition for preventing hair loss, promoting hair growth and increasing hair thickness of the present invention stimulates the skin was measured by applying time-accumulated patches. The patching method used a pin chamber (Finn chamber, Epitest Ltd, Finland). After 15 µl of each of the above skin external preparations was dripped into the chamber, patching was performed. The degree of the reaction shown on the skin each time was scored using Equation 2 below, and the results are shown in Table 8 below.

In the reactivity, ± is given a score of 1 point, + is given a score of 2, and ++ is given a score of 4, and when the average reactivity is less than 3, it is judged as a safe composition.

test substance
Number of subjects who responded
average
reactivity
1 week
2 weeks
3 weeks
Primary
Secondary
tertiary
4th
5th
6th
7th
8th
9th
±+ ++
±+ ++
±+ ++
±+ ++
±+ ++
±+ ++
±+ ++
±+ ++
±+ ++
control
(Squalene)
One - -
0 - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
0.09
[Test group 1]
0 - -
0 - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
0.00

In Table 8, the number of persons corresponding to ±, + and ++ in Test Group 1 was 0, 0, and 1, respectively, and the average reactivity was 0.00 and 0.00, respectively. Therefore, since the average reactivity shows a reactivity of 3 or less, the composition for preventing hair loss, promoting hair growth and increasing hair thickness according to the present invention was determined to be a safe material for human skin that does not show a distinct cumulative stimulation pattern.

Examples of formulations for the composition of the present invention are exemplified below.

<Formulation Example 1> Preparation of hair shampoo

A hair shampoo containing a composition for preventing hair loss, promoting hair growth and increasing hair thickness having a composition as shown in Table 9 was prepared.

Ingredient name
content(%)
Purified water
45.75
Polyquaternium-10
0.20
Carbomer
0.15
Disodium Cocoamphodiacetate
2.00
Sodium Laureth Sulfate
10.00
lauryl hydroxy sultaine
2.00
cetyl alcohol
0.50
Disodium EDIT
0.05
trihydroxystearin
0.05
Polyquaternium-7
0.30
Cocamide Methyl M.A.
2.50
Phenoxyethanol
0.50
caprylyl glycol
0.10
Spices
0.70
Composition for preventing hair loss, promoting hair growth and increasing hair thickness of Example 1
35.20

<Formulation Example 2> Preparation of hair treatment

A hair treatment containing a composition for preventing hair loss, promoting hair growth and increasing hair thickness having a composition as shown in Table 10 was prepared.

Ingredient name
content(%)
Purified water
39.40
Trehalose
1.00
Hydroxyethyl Cellulose
0.30
glycerin
3.00
Cetrimonium Chloride
0.20
Behentrimonium Chloride
2.40
isopropyl alcohol
0.60
propylene glycol
1.10
Steatrimonium Chloride
1.50
cetyl alcohol
5.00
behenyl alcohol
1.00
Butylene glycol dicaprylate/dicaprate
6.00
dimethicone
2.00
Composition for preventing hair loss, promoting hair growth and increasing hair thickness of Example 1
35.50
Spices
0.50
propanediol
0.40
caprylyl glycol
0.08
Ethylhexylglycerin
0.02

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Prevention of hair loss, promotion of hair growth and hair growth containing a complex oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, sagebrush, guava leaf, hawthorn, gwangnae, black bean, calamus leaf and goji berry as an active ingredient A hair care composition for increasing thickness, comprising:
The oriental herbal complex extract contains 40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of extract of oleracea extract, 70 to 80 parts by weight of oleracea extract with respect to 100 parts by weight of extract of oriental medicinal herb based on 100 parts by weight of extract. 80 parts by weight, 70 to 80 parts by weight of chrysanthemum extract, 70 to 80 parts by weight of trifolieae extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of sauerkraut extract, 40 to 60 parts by weight of Kwangnam extract, 90 to A hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness, comprising 110 parts by weight, 70 to 80 parts by weight of iris leaf extract, and 20 to 30 parts by weight of Goji berry extract. According to claim 1,
The oriental herbal complex extract, Eoseongcho, perilla leaf, green tea, licorice, Machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, haw, gwangnamu, black bean, calamus leaf, and goji berry about 1 to 40 times the weight of each sample of water , methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohol of 1 to 5 carbon atoms including butanol (butanol) or 1:0.1 to 1:10 mixing ratio (kg / ℓ) After ultrasonic extraction for 2 to 7 days with a mixed solvent of water and methanol having , A hair care composition for preventing hair loss, promoting hair growth and increasing hair thickness, characterized in that it is prepared by obtaining and then mixing a trifolieae extract, a guava leaf extract, a sewage extract, a gwang tree extract, a black bean extract, an iris leaf extract and a goji berry extract. Prevention of hair loss, promotion of hair growth and hair growth containing a complex oriental herbal complex extract consisting of eoseongcho, perilla leaf, green tea, licorice, machihyeon, chrysanthemum, chrysanthemum, sagebrush, guava leaf, hawthorn, gwangnae, black bean, calamus leaf and goji berry as an active ingredient As a cosmetic composition for increasing the thickness,
40 to 60 parts by weight of perilla leaf extract, 40 to 60 parts by weight of green tea extract, 70 to 80 parts by weight of licorice extract, 70 to 80 parts by weight of Machisin extract, 70 to 80 parts by weight of orchid extract, 70 to 80 parts by weight of chrysanthemum extract based on 100 parts by weight of eoseongcho extract 70 to 80 parts by weight, 70 to 80 parts by weight of trifolieae extract, 20 to 30 parts by weight of guava leaf extract, 90 to 110 parts by weight of sauerkraut extract, 40 to 60 parts by weight of Gwangnam extract, 90 to 110 parts by weight of black bean extract, iris leaf A cosmetic composition for preventing hair loss, promoting hair growth and increasing hair thickness, comprising as an active ingredient an oriental herbal complex extract consisting of 70 to 80 parts by weight of extract and 20 to 30 parts by weight of Goji berry extract. 4. The method of claim 3,
The cosmetic composition is hair shampoo, hair conditioner, hair essence, hair nutrition, hair oil, hair gel, hair conditioner, hair treatment or hair dye, hair loss prevention, hair growth promotion and hair thickness increase cosmetic composition. 4. The method of claim 3,
The oriental herbal complex extract, Eoseongcho, perilla leaf, green tea, licorice, Machihyeon, chrysanthemum, chrysanthemum, trifolieae, guava leaf, haw, gwangnamu, black bean, calamus leaf, and goji berry about 1 to 40 times the weight of each sample of water , methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohol of 1 to 5 carbon atoms including butanol (butanol) or 1:0.1 to 1:10 mixing ratio (kg / ℓ) After ultrasonic extraction for 2 to 7 days with a mixed solvent of water and methanol having , A cosmetic composition for preventing hair loss, promoting hair growth and increasing hair thickness, characterized in that it is prepared by obtaining and then mixing the extracts of triticale extract, guava leaf extract, hawthorn extract, gwang tree extract, black bean extract, iris leaf extract and goji berry extract.