KR101849924B1 - Composition Having Promoting Effect on Induction of Black Hair and Inhibitory Effect on Induction of White Hair - Google Patents

Abstract

A blackening and anti-whitening composition is disclosed. The present invention includes 20 to 30 parts by weight of red ginseng extract, 70 to 95 parts by weight of a strawberry extract, and 1 to 3 parts by weight of a black bean extract. According to the present invention, there is provided a composition for preventing hair loss and preventing hair whorl using only pure natural materials without using chemical additives at all.

Description

[0001] The present invention relates to a composition for preventing and inhibiting the onset of black hair,

The present invention relates to a composition for preventing and inhibiting the onset of black cohosh, and more particularly to a composition for preventing and inhibiting the onset of black cohosh by using a natural substance.

The main component of the hair is protein, and it is composed of three parts: the skin of the eye, the fur, and the water quality. The follicles surrounding the waters are hair thickest (80 ~ 90% by weight), melanin pigment and lipid membrane and TRP-1 (Tyrosinase Related Protein-1), TRP-2 (Tyrosinase Related Protein- It contains a granola called melanomas.

The melanin pigment is synthesized in the melanocyte, the organelle in the melanocyte, and is transferred to the surrounding keratinocyte through the resin dendrites of the melanocyte.

In the synthesis pathway of melanin, tyrosinase is involved first in the stage where amino acid tyrosine is oxidized and then waveguide is oxidized again to generate dopaquinone. After that, the reaction proceeds auto-oxidatively, but TRP-1 and TRP- 2 is involved and is known to accelerate the reaction.

The color of hair is determined by the factors such as the degree of the type and distribution of the eumelanin and peomelanin, which are formed from the amino acid tyrosine, and reveals the hair color unique to each person. Eumelanin is the most common and darkest pigment, making brown and black hair color, while peomelanin is a lighter hue that makes blond hair color.

Gray hair is a phenomenon of physiological aging, which is caused by a decrease in the number of hair melanocytes and a decrease in the production of melanin by the degradation of melanocytes.

Dyeing agents for hiding gray hair have been extensively studied and widely marketed. However, black hair dyeing has to be dyed again by the growth of gray hair in a transient manner, and oxidized black hair dyeing, which is currently widely used, Damage the skin. Accordingly, various studies have been conducted on the anti-whitening agent for preventing the occurrence of white feathers, for example, Korean Patent Publication No. 2002-0059906.

However, conventional anti-shudder agents generally contain a large amount of chemical substances harmful to human body, and in the case of the anti-shudder agent using a natural substance, sufficient effect for preventing white fever and causing black hair has not been confirmed.

Accordingly, it is an object of the present invention to provide a composition for preventing or inhibiting the onset of black cohosh.

In order to accomplish the above object, the present invention provides a composition for preventing and inhibiting the production of black feather, comprising 20 to 30 parts by weight of red ginseng extract; 70 to 95 parts by weight of a strawberry extract; And 1 to 3 parts by weight of a black bean extract.

Preferably, it further comprises 10 to 20 parts by weight of a blueberry extract.

Further, it is characterized by further comprising 1 to 3 parts by weight of a cactus extract.

In addition, it is characterized by further comprising 1 to 3 parts by weight of a Japanese persimmon tree extract.

Further, it is characterized by further comprising 3 to 7 parts by weight of an extract of Bacillus subtilis.

Further, it is characterized by further comprising 1 to 3 parts by weight of the extract of Sasa extract.

Further, it is characterized by further comprising 1 to 3 parts by weight of green tea extract.

According to the present invention, there is provided a composition for preventing and inhibiting the formation of black cohosh using a natural substance.

FIG. 1 is a photographic view showing changes in hair condition of a user who uses the compositions for preventing and inhibiting the production of black feather and white hair according to the present invention for 5 months. FIG.

Hereinafter, the present invention will be described in detail with reference to the drawings. It is to be noted that the same elements among the drawings are denoted by the same reference numerals whenever possible. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

The anti-whitening and anti-whitening composition according to the present invention is prepared by mixing red ginseng extract, bamboo fruit extract, and black bean extract. Specifically, 20 to 30 parts by weight of red ginseng extract, 70 to 95 parts by weight of bergamot extract, 3 to 7 parts by weight of the composition are mixed to prepare a composition.

- Red ginseng extract -

Ginsenoside of red ginseng extract is effective in promoting hair growth. When the amount of red ginseng extract is less than 20 parts by weight, there is a problem that the effect of promoting hair growth is somewhat low in the composition ratio with the bran extract, and the red ginseng extract is more than 30 parts by weight There is a case where the sediment is increased and the fragrance thereof becomes too strong and there is a problem in feeling. Therefore, in the present invention, the red ginseng extract is mixed in 20 to 30 parts by weight.

- Strawberry Extract -

When the extract is less than 70 parts by weight, there is a problem in that the effect of promoting hair growth is somewhat low in the composition ratio with red ginseng extract. When the extract of bananas is more than 95 parts by weight There is a case where the sediment is increased and the fragrance thereof becomes too strong and there is a problem in usability. Therefore, in the present invention, 70 to 95 parts by weight of the berry extract is mixed.

- Black bean extract -

Black bean extract is one of the most amino acids in black beans. Arginine, which is a necessary ingredient for hair growth, is a metabolic precursor of a neutral acicide that promotes hair growth. Cysteine, an essential component of hair growth, It is effective in blackening hair.

On the other hand, when the black bean extract is less than 3 parts by weight, there is a problem in the efficacy of the pharmacological effect manifestation. When the black bean extract is more than 7 parts by weight, the expression of the pharmacological effect of the red ginseng extract and the The black bean extract is mixed in an amount of 3 to 7 parts by weight in the present invention.

In carrying out the present invention, in addition to the red ginseng extract, the bananas extract, and the black bean extract, the extracts of blueberry, cactus, quercus, leaf, root extract, green tea extract, Extracts of henna, ginseng extract, ginseng extract, ginseng extract, seaweed extract, licorice extract, espinoziella, locus hazel, rhododendron extract, birch extract, bupyeongcho extract, bicycle extract, nicotinamide, By further mixing, the effect of preventing the occurrence of the black pigments and preventing the white pigments may be increased.

- Blueberries  extract-

The blueberry extract has an effect on strengthening the hair. When the blueberry extract is less than 10 parts by weight, there is a problem of the effectiveness of the expression of the pharmacological effect. When the blueberry extract is more than 20 parts by weight, There is a problem in that the expression of the pharmacological effect of the extract and the branberry extract is relatively limited. Therefore, in the present invention, the blueberry extract is mixed at 10 to 20 parts by weight.

- Cactus extract -

When the cactus extract is used in an amount of less than 1 part by weight, there is a problem in the effectiveness of the expression of the pharmacological effect. When the cactus extract is more than 3 parts by weight, the pharmacological effect of the red ginseng extract and the cactus , The cactus extract is mixed in an amount of 1 to 3 parts by weight in the present invention.

- Leaf Extract -

When the extract is less than 1 part by weight, there is a problem in the efficacy of the expression of the pharmacological effect. In the case where the extract of the Leptospira is more than 3 parts by weight There is a problem in that the expression of the pharmacological effect of the red ginseng extract and the bamboo fruit extract is relatively limited. Therefore, in the present invention, 1 to 3 parts by weight of the Leaf Leaf Extract is mixed.

- Saururus chinensis leaf / root extract -

The Saururus chinensis leaf / root extract is effective for improving inflammation and blood circulation. When the Saururus chinense leaf / root extract is less than 1 part by weight, there is a problem in the effectiveness of the expression of pharmacological effect. When the Saururus chinensis leaf / root extract is more than 3 parts by weight , There is a problem in that the expression of pharmacological effects of the red ginseng extract and the bananas extract is relatively limited. Therefore, in the present invention, the Saururus chinensis leaf / root extract is mixed with 1 to 3 parts by weight.

- Sasa root / Stem Extract -

When the extract of Sasa roots / stem extract is less than 1 part by weight, there is a problem in the effectiveness of the expression of pharmacological effect. When the extract of Sasa roots / stem extract is more than 3 parts by weight There is a problem that the expression of the pharmacological effect of the red ginseng extract and the bananas extract is relatively limited. Therefore, in the present invention, 1 to 3 parts by weight of Sasa root / stem extract is mixed.

- Green tea extract -

When the green tea extract is less than 1 part by weight, there is a problem in the effectiveness of the expression of the pharmacological effect. When the green tea extract is more than 3 parts by weight, the red ginseng extract, , The green tea extract is mixed in an amount of 1 to 3 parts by weight in the present invention.

- iris extract -

The iris extract has an effect of strengthening and softening the hair. Thus, by adding 0.1 to 0.5 parts by weight of iris extract to the present invention, it is possible to enhance the effect of prevention of the occurrence of the black feather and the prevention of the white fever.

- Henna extract -

The henna extract has the effect of calming the scalp heat. In the present invention, by adding 0.1 to 0.5 part by weight of the henna extract, it is possible to increase the effect of preventing the black feather and the white hair.

- Kiss  extract-

The gold leaf extract has an effect on scalp trouble. In the present invention, by adding 0.1 to 0.5 part by weight of Gold leaf extract, the effect of preventing black feather and preventing white fever can be increased.

- GoSam  extract-

The extracts of gosam are effective for reducing irritation and increasing blood flow. Thus, by adding 0.1 to 0.5 parts by weight of extract of gosam extract, the effect of preventing the occurrence of black blood and preventing white hair can be enhanced.

- Angelicae extract -

The extract of Angelicae Radix guinea pigs is effective for alleviating inflammation and improving blood circulation. Thus, by adding 0.1 to 0.5 parts by weight of Angelica giganta extract to the present invention, it is possible to increase the effect of preventing the occurrence of the black cohosh and the anti-whorl.

- Seaweed extract -

Seaweed extracts are effective to make hair elastic. Thus, by adding 0.1 to 0.5 parts by weight of seaweed extract, it is possible to increase the effect of prevention of black feather and white hair.

- Licorice extract -

The licorice extract has an effect on seborrheic dermatitis. Thus, by adding 0.1 to 0.5 part by weight of the licorice extract to the present invention, it is possible to increase the effect of preventing the occurrence of the black feather and the black spot.

- Espinosilia -

Espinozillia is effective in reducing dandruff and preventing hair loss, promoting hair growth, and alleviating the bruising scalp. Thus, by adding 0.1 to 0.5 parts by weight of Espinolylaa in the practice of the present invention, .

- Location Hazel -

The position hazel is effective for the control of sebum, and in the practice of the present invention, by adding 0.1 to 0.5 parts by weight of the position haze, it is possible to increase the effect of prevention of the occurrence of the black feather and the prevention of the white spot.

- Horsetail extract -

In the present invention, the addition of 0.1 to 0.5 part by weight of the extract of Opuntia ficus may increase the effect of preventing the occurrence of the black cohosh and the prevention of the white cohosh.

- Birch extract -

The birch extract has an effect of cleansing the scalp. Thus, by adding 0.1 to 0.5 parts by weight of the birch extract in the practice of the present invention, it is possible to increase the effect of preventing the occurrence of the black feather and the white hair.

- Bupyeongcho extract -

The extract of Buchungcho has an efficacy for antiinflammation and scalp calming. Thus, by adding 0.1 to 0.5 parts by weight of Puchipcheon extract to the present invention, it is possible to increase the effect of preventing the occurrence of the black feather and the white hair.

- A car  extract-

The extract of the present invention is effective for antiinflammation and scalp calming. Thus, by adding 0.1 to 0.5 part by weight of the extract of a Korean lotus flower to the present invention, it is possible to increase the effect of preventing the occurrence of black feather and white hair.

- Nicotinic acid Amide -

Nicotinic amide is a complex of vitamin B3, and is effective for preventing hair loss and promoting hair growth. Thus, by adding 0.1 to 0.5 part by weight of nicotinic acid amide in the practice of the present invention, the effect of preventing hair whorl and preventing hair whorl can be enhanced.

- Objective extract -

The extract of the present invention is effective for improving scalp trouble. Thus, by adding 0.1 to 0.5 part by weight of the extract of the extract of the present invention, the effect of preventing the occurrence of black feather and preventing white fever can be increased.

- Small palmito -

Sopalmito is effective for improving blood circulation. Thus, by adding 0.1 to 0.5 parts by weight of bovine palmitoate in the practice of the present invention, the effect of prevention of black cohosh and prevention of whitehead can be increased.

- Bank extract -

The extract of the bank is effective in preventing hair loss and white moth. In the present invention, by adding 0.1 to 0.5 part by weight of the bank extract, it is possible to increase the effect of prevention of black cohosh and prevention of white moth.

As shown in the following Examples and Experimental Examples, the inventors of the present invention examined the activity of accelerating the production of melanin in B16F10 cells by extracting dried and whole plants with water and 70% ethanol. As a result, The water extracts showed 32.5% and the 70% ethanol extracts had 26.3% melanogenesis promoting activity compared to the control group.

These cell test results showed similar tendency in animal experiments. Also, in the clinical evaluation performed using the scalp nutrient prepared by mixing the water extract of ranitum tuberculosis and the water extract of ranitic ginseng, the effect of promoting the proliferation of the black blood was evident. The present invention is provided based on these experimental results.

In the present specification, the term "bananas extract" means any extract obtained by immersing the bananas, which are the object of extraction, in water containing distilled water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol or butanol or a mixed solvent thereof, The extract and the crude extract thereof are understood to mean an extract obtained by fractionating the above-listed solvents.

The extraction means includes a concentrated liquid extract or solid form extract from which the extraction solvent has been removed. Since the extraction method does not depend on the extraction method, as long as it is extracted through the step of immersing the extraction object in the extraction solvent, , Reflux, heating, ultrasonic irradiation, and the like can all be applied. Nevertheless, it preferably means a bananas extract obtained by using water, ethanol or a mixed solvent thereof.

In the present specification, the term "red ginseng extract", "black bean extract", "blueberry extract", "cactus extract", " , The extracts of Angelica keiskei keiskei, seaweed extract, licorice extract, rhododendron extract, birch extract, bupyeongcho extract, bicycle extract, and phosphorus phosphorus can be defined in the same manner as the above-mentioned "bananus extract" .

In the present specification, "red ginseng" in red ginseng extract refers to red ginseng obtained by boiling ginseng and drying it. More specifically, after ginseng is germinated, it is dried in sun or shade so as to have a moisture content of about 10 to 15 wt% .

In addition, in the following Examples and Experimental Examples of the present invention, it was obvious that the use of the bananas extract and the red ginseng extract would be similar to the use of the bananas powder and the red ginseng powder, Alternatively, it may be used with the strawberry extract / red ginseng extract and the strawberry powder / red ginseng powder.

In the present specification, the term "active ingredient" means a component that alone exhibits the desired activity or can exhibit activity together with a carrier which is itself inactive.

Meanwhile, in the composition for preventing blood coagulation or anti-whiplash of the present invention, the branberry extract may be contained in any amount (effective amount) as long as it can exhibit the activity of preventing the occurrence of a black cohosh or anti- Will be determined within the range of from 70 parts by weight to 75 parts by weight based on the total weight of the composition.

Herein, "effective amount" refers to the amount of the active ingredient capable of promoting the prevention of black cohosh or white hair.

The composition for preventing a black cohosh or anti-whiplash of the present invention, in a specific embodiment, can be identified as a hair cosmetics composition.

When the hair tonic or anti-whiplash composition of the present invention is identified as a hair cosmetic composition, the cosmetic composition may be manufactured into various formulations such as emulsions, lotions, creams (oil-in-water type, water- Suspensions (anhydrous and aquatic), anhydrous products (oil and glycol), gels, powders, and the like.

The composition of the present invention for preventing or preventing blood coagulation may contain an acceptable carrier in hair cosmetic preparations in addition to the active ingredient, a bananas extract.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, preservatives, wetting agents, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents, flavoring agents and diluents.

Specific compounds or compositions that can be used as the above-mentioned alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosifier, emulsion, stabilizer, ultraviolet screening agent, coloring agent, A person skilled in the art can select and use the appropriate compound or composition.

Examples of alcohols include water-soluble polyhydric alcohols such as higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, sorbitol and polyethylene glycol. Examples of the oils include avocado oil, palm oil, Examples of the preservative include ethylparaben and butylparaben. Examples of the moisturizing agent include hyaluronic acid, chondroitin sulfate, and pyrrolidonecarboxylic acid salt. Examples of diluents include ethanol, isopropanol, and the like .

The composition for preventing a black cohosh or anti-whiplash of the present invention can be identified as a shampoo composition in another specific embodiment.

The shampoo composition of the present invention can be prepared by incorporating a carrier which is acceptable in shampoo formulations other than the active ingredient, the serpentine extract, when the composition for preventing hair whitening or preventing whitening of the present invention is identified as a shampoo composition. Emulsifiers, thickeners, perfumes, hydrotropes, preservatives, pH adjusting agents, bactericides, anti-dandruff agents, tonic agents, moisturizers and the like.

The surfactant, the foam enhancer, the emulsifier, the thickener, the hydrotrope, the preservative, the pH adjuster, the bactericide, the anti-dandruff agent and the tonic agent may be those known in the art. There can be used Alcohol Ethoxy Sulfate (Sodium Lauryl Ethoxylated Sulfate), Alkyl Sulfate, Acyl Sarsosinate, Acyl Grutaminate, Phosphoric Acid, Sucrose Ester and the like. Sodium hydroxide, sodium starch, starch, and the like can be used. As the perfume agent, ethyl alcohol, butyl alcohol, propylene glycol and the like can be used. As the humectant, glycerin Etc. may be used.

The composition for preventing a black cohosh or anti-whiplash of the present invention can be identified as a soap composition in another specific embodiment.

When the composition of the present invention is recognized as a soap composition, the soap composition of the present invention can be prepared by incorporating the active ingredient into a soap base material, and can be prepared as an additive including a skin moisturizer, an emulsifier, a water softener and the like .

Examples of the soap base material include vegetable oils such as palm oil, palm oil, soybean oil, perm free oil, olive oil and palm kernel oil or animal fats such as tallow, lard, sunshine and fish oil. Examples of the skin moisturizers include glycerin, erythritol, polyethylene glycol , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like. Examples of the emulsifier include natural oils, wax fatty alcohols, hydrocarbons, May be used. As the water softening agent, tetrasodium ethylenediate and the like may be used.

The soap composition of the present invention may further contain, as an additive, an antibacterial agent, a dispersant, a foam inhibitor, a solvent, a scouring inhibitor, a corrosion inhibitor, a fragrance, a dye, a sequestering agent, an antioxidant and an antiseptic.

The composition of the present invention can be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may be formulated into a wide variety of dosage forms, including pharmaceutically acceptable carriers, excipients and the like, in addition to the active ingredient, and may be formulated into a wide variety of dosage forms such as a localized preparation such as cream, lotion, ointment (semi-solid external drug), micro loo emulsion, (TTS) (e.g., patches, bandages, etc.), and the like.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (e.g. corn starch, potato starch, etc.), cellulose, derivatives thereof (e.g. sodium carboxymethylcellulose, ethylcellulose, etc.), malt, gelatin, talc, (E.g., peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g., propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g., TWEENS), wetting agents (For example, sodium lauryl sulfate), a coloring agent, a flavoring agent, a stabilizer, an antioxidant, a preservative, water, a saline solution, and a phosphate buffer solution. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

The pharmaceutical compositions of the present invention may be administered orally or parenterally, and preferably topically.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

In another specific embodiment, the composition of the present invention can be identified as a food composition.

The food composition of the present invention can be produced as a health supplement food, a special nutrition supplement food, a functional beverage and the like.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of bamboo fruit extract and red ginseng extract

< Example  1> Manufacture of cranberry extract

&Lt; Example 1-1 > Preparation Example 1 of a bananas extract

5 kg of water was added to 1 kg of dried bamboo shoots, and the mixture was extracted with hot water at 70 ~ 90 ° C for about 6 hours to obtain an extract. The extract was filtered with a filter paper of 2 탆 and concentrated under reduced pressure to obtain a high- .

&Lt; Example 1-2 > Preparation Example 2 of a Strawberry Extract

1 kg of dried bamboo shoots was immersed in 2 liters of 70% ethanol for 24 hours at room temperature to obtain an extract. The extract was filtered with a filter paper having a size of 2 탆, and the filtrate was concentrated under reduced pressure to prepare a solid form of bananas extract.

< Example  2> Production of red ginseng extract

<Example 2-1> Preparation of red ginseng extract Example 1

The extract was filtered through 2 ㎛ filter paper, and the filtrate was concentrated under reduced pressure to obtain a solid red ginseng extract .

<Example 2-2> Preparation of red ginseng extract Example 2

1 kg of three red ginseng was immersed in 2 liters of 70% ethanol for 24 hours at room temperature to obtain an extract. The extract was filtered with a filter paper of 2 탆, and the filtrate was concentrated under reduced pressure to prepare a solid red ginseng extract.

< Example  3> Manufacture of black bean extract

&Lt; Example 3-1 & gt ; Preparation Example 1 of black bean extract

After adding 5 ℓ of water to 1 kg of black beans and extracting with hot water extraction at 70 ~ 90 ℃ for about 6 hours, the extract was filtered with 2 ㎛ filter paper, and the filtrate was concentrated under reduced pressure to obtain a solid black Soybean extract was prepared.

&Lt; Example 3-2 & gt ; Preparation Example 2 of black bean extract

One kg of black beans was immersed in 2 liters of 70% ethanol for 24 hours at room temperature to obtain an extract. The extract was filtered with a filter paper having a size of 2 탆, and the filtrate was concentrated under reduced pressure to prepare a solid black bean extract.

< Experimental Example > Black hair  Induction stimulation effect experiment

Experimental Example 1 Melanin production promoting activity experiment

<Experimental Example 1-1> Measurement of melanin production promotion rate

Mouse-derived B16F10 cells were cultured in DMEM (Dulbeccos modified Eagles media) supplemented with 10% PBS (fetal bovine serum) at 37 ° C and 5% CO ₂ .

Cells were cultured in a 24-well plate at a concentration of 1 × 10 ⁵ cells / well for 18 hours and added to the cell culture medium in the same manner as in [Table 1] below (samples of the extracts of the above- ) Was treated at a concentration of 100 μg / mL and cultured for 3 days. The culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH, and absorbance was measured at 405 nm. As a control group, the melanin production of the sample was measured according to the following equation, in comparison with the melanin content in the control group.

Melanin production promotion rate (%) = (absorbance of each sample / absorbance of control group) x 100

Melanin production promoting activity (%) sample Melanin production promoting activity (%) Control group (untreated group) 100 The extract of Example 1-1 132.5 ± 4.3 ** The extract of Example 1-2 126.3 ± 2.5 ** A mixture of each of the extracts of Example 1-1, Example 2-1, and Example 3-1 147.2 ± 3.7 ** A mixture of each of the extracts of Example 1-2, Example 2-2, and Example 3-2 143.9 ± 5.2 ** - All test results were expressed as mean ± SD, and the significance was verified by Student's t-test (* p <0.05, ** p <0.01)

The results in Table 1 show that melanin production promoting activity of the bananas extract is enhanced when mixed with red ginseng extract and black bean extract.

&Lt; Experimental Example 1-2 >

To determine whether the melanogenesis promoting activity was the result of promoting cell proliferation, the cell proliferation rate was evaluated using the MTT method.

B16F10 cells were adjusted to 1 × 10 ⁵ cells / mL using DMEM medium supplemented with 10% FBS, and then inoculated on a 24-well plate. After 18 hours of culture, the sample of [Table 1] 1-1> and cultured in a 10% CO ² cell incubator at 37 ° C for 3 days. 200 쨉 l of an MTT solution prepared at a concentration of 2 mg / ml of PBS was added thereto and cultured for 4 hours under the same culture conditions. The culture was removed and 200 μl of DMSO was added to each well. Absorbance was measured at 570 nm.

The cell proliferation rate was calculated according to the following formula and the results are shown in Table 2 below.

Cell proliferation promotion rate (%) = (absorbance of each sample / absorbance of control group) x 100

Cell Proliferation Promoting Activity (%) sample Cell Proliferation Promoting Activity (%) Control group (untreated group) 100 The extract of Example 1-1 97.5 The extract of Example 1-2 98.7 A mixture of each of the extracts of Example 1-1, Example 2-1, and Example 3-1 102.4 A mixture of each of the extracts of Example 1-2, Example 2-2, and Example 3-2 99.2

The results of the above Table 2 show that the results of the above Table 1 are not the result of the cell proliferation promoting activity.

<Experimental Example 2> Animal experiments to promote Japanese black inducing activity

Biosci. Biotechnol. Biochem., 69 (5): 873 (2005)), which is a modified version of C ₃ H / Hej mouse, .

Specifically, four-week-old female C3H / Hej mice (SLC, Japan) were purchased and subjected to an adaptation period for one week. Then, mice were divided into four test groups and one control group by five mice. Ketamin (Huons, Korea) and Rompun , Germany), and the hair was removed with a wax hair removal kit (Reckitt Benckiser, England). The samples of Table 1 were dissolved in vehicle (AOO, acetone: olive oil = 4: 1) at 5.0% (w / v) and applied to the back region for 12 weeks. After the application, hair was collected from the dorsal area, melanin was dissolved in 10% DMSO + 1 N NaOH solution, and absorbance was measured at 405 nm with a microplate reader.

As a control group, the melanin production of the sample was measured according to the following equation, in comparison with the melanin content in the control group.

Melanin production promotion rate (%) = (absorbance of each sample / absorbance of control group) x 100

The results are shown in Table 3.

Melanin production growth rate sample Increase in melanin production (%) Control group (untreated group) 100 The extract of Example 1-1 121.7 ± 5.3 ** The extract of Example 1-2 114.4 ± 4.3 ** A mixture of each of the extracts of Example 1-1, Example 2-1, and Example 3-1 129.4 ± 2.9 ** A mixture of each of the extracts of Example 1-1, Example 2-1, and Example 3-1 125.5 ± 4.2 ** - All test results were expressed as mean ± SD, and the significance was verified by Student's t-test (* p <0.05, ** p <0.01)

The results of the above Table 3 were similar to those of the above Table 1, and the addition of the red ginseng extract and black bean extract to the bananas extract showed a higher effect of increasing melanin production.

<Experimental Example 3> cause clinical evaluation of the Japanese black

Clinical evaluations were carried out as follows to confirm the effect of the extract of the above Example on the blood coagulation promoting effect.

First, as shown in [Table 4] below, a scalp nutritional composition containing the extracts of the <Example 1-1> and the red ginseng of <Example 2-1> was prepared, and a male adult For 5 months after shampooing, the dose was applied to the scalp once or twice a day.

The results are shown in the photographs before and after use in FIG. 1, and it can be confirmed that the black hair increased remarkably before and after use.

Components and content of scalp nutritional composition ingredient Content (% by weight) &Lt; Example 1-1 > 7.0 <Example 2-1> Red ginseng extract 3.0 Purified water Balance

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention.

Furthermore, the terms used in the present invention are used only to describe specific embodiments and are not intended to limit the present invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Claims (7)

20 to 30 parts by weight of red ginseng extract;
70 to 95 parts by weight of a strawberry extract;
3 to 7 parts by weight of black bean extract;
0.1 to 0.5 parts by weight of Angelica giganta extract;
0.1 to 0.5 parts by weight of seaweed extract; And
0.1 to 0.5 parts by weight of licorice extract
&Lt; / RTI &gt;
The method according to claim 1,
10 to 20 parts by weight of a blueberry extract.
The method according to claim 1,
Lt; RTI ID = 0.0 &gt; 1, &lt; / RTI &gt; 3 parts by weight of a cactus extract.
The method according to claim 1,
Wherein the composition further comprises 1 to 3 parts by weight of a cinnabar and leaf extract.
The method according to claim 1,
Wherein the composition further comprises 1 to 3 parts by weight of Saururus chinensis leaf and root extract.
The method according to claim 1,
Wherein the composition further comprises 1 to 3 parts by weight of a root extract and a root extract.
The method according to claim 1,
1 to 3 parts by weight of a green tea extract.

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