KR100834108B1 - Composition for preventing falling out of the hair and promoting growth of the hair - Google Patents

Abstract

A composition is provided to show hair loss preventing effect and hair growth effect without causing any side effects even for a long use time, thereby being usefully applied as a cosmetic for preventing the hair loss and promoting the hair gross. A composition for preventing hair loss and promoting hair growth comprises 5-15 parts by weight of an extract of Panax ginseng C.A Meyer, 4-5 parts by weight of an extract of Saposhnikovia divaricata Schiskin, 2-5 parts by weight of an extract of Schizonepeta tenuifolia, 0.5-15 parts by weight of an extract of baicalin extracted from Scutellaria baicalensis, 1-5 parts by weight of an extract of Sophora flavescens, 0.1-5 parts by weight of an extract of Glycyrrhiza uralensis, 0.5-5 parts by weight of an extract of Angelica gigas Nakai, 5-15 parts by weight of an extract of Porphyra tenera, 0.5-5 parts by weight of an extract of Pleuropterus multflorus, 10-20 parts by weight of an extract of Cimicifuga davurica maxim roots, and 1-5 parts by weight of an extract of Perilla frutescens var. acuta kudo. Further, the extract of Panax ginseng C.A Meyer, the extract of Saposhnikovia divaricata Schiskin, the extract of Sophora flavescens, the extract of Pleuropterus multflorus and the extract of Perilla frutescens var. acuta kudo are extracted from a mixture solvent of water and ethanol which is mixed at a weight ratio of 1:1 to 10.

Description

Composition for preventing falling out of the hair and promoting growth of the hair}

Figure 1 shows the structure and ¹ H NMR spectrum of Baikal contained in the composition according to the present invention,

Figure 2 shows the results of HPLC analysis of the baccalin contained in the composition according to the present invention (a) is a standard material, (b) is a result of the material prepared in the preparation of the present invention,

Figure 3 is a photograph showing the hair growth promoting effect according to Experimental Example 2 of the present invention,

Figure 4 is a photograph showing the hair root cell activity effect in the skin section according to Experimental Example 3 of the present invention (a) is a solvent control group, (b) Preparation Example 9 extract (10% by weight), (c) is a preparation example 9 extract (5% by weight), (d) is the result of treatment with minoxidil (5% by weight).

The present invention relates to a composition for preventing hair loss and promoting hair growth.

Hair protects and protects the body from external stimuli such as buffering and external stimuli such as ultraviolet rays, temperature, and friction, and absorbs heavy metals such as arsenic, mercury, and zinc that are unnecessary to the body and releases them to the body. It is an important aesthetic factor in the expression of external images.

Hair is only a mammal, and as an appendage to the skin, hair development starts from 9 to 12 weeks of birth and is inherited by parental inheritance. Hair grows in follicles. The hair is divided into three periods according to the hair cycle (anagen, catagen, and telogen). Hair grows only in the growth phase, and each hair growth cycle is independent and has its own cycle. The whole picture does not fall off at one time. During the resting period, there are 70 to 120 hairs per day, and naturally drop out every day. Hair cycles are divided into five to six years of growth, two to three weeks of degeneration, and two to three months of rest. Hair growth is influenced by various internal and external factors, such as nutritional status of the individual, hormonal temperature consistent systemic and disease, and various chemicals. Especially, when the epidermal overgrowth of the scalp is caused by some cause, It is known.

These days, the physical rhythm is broken due to various causes such as environmental, genetic, mental, and physical, and the hair follicles as well as the scalp are greatly affected. Because of this, hair damage is basic, and it becomes dry or sensitive, and it appears in various forms such as hair loss.

Accordingly, research has been conducted on compositions that can improve the condition of the scalp and hair by preventing abnormalities of various scalp and hair including hair loss. Such compositions should exhibit excellent hair loss prevention, hair growth, and wool effects.

In addition, since the product of the composition used for the scalp and hair is frequently used for a long time, it should not show side effects such as irritation or damage. However, related products on the market are insufficient to show the expected improvement of the scalp and hair, and the main ingredient is often a chemical, which often causes side effects on the scalp.

Therefore, there is a need for the development of a composition for preventing hair loss and promoting hair growth that is excellent in preventing hair loss and promoting hair growth and having no side effects even in long-term use.

It is an object of the present invention to provide a composition for preventing hair loss and promoting hair growth and having no side effects even when used for a long time, and for promoting hair growth, in particular, by activating hair follicle cells of the scalp, the hair growth promoting effect is excellent. And, it is an object to provide a composition for preventing hair loss and hair growth without any side effects even using for a long time using only components derived from natural materials.

Accordingly, the present inventors developed a composition containing an optimal ratio of Baikal extracted from natural products such as red ginseng, windproof, hyungae, ginseng, licorice, donkey, seaweed, sewage, horse riding, leaflets and gold, thereby causing abnormal symptoms of the scalp and hair. Invented a composition for preventing hair loss and promoting hair growth, which shows excellent improvement effect for all and does not cause side effects even in long-term use.

The present invention relates to a composition for preventing hair loss and promoting hair growth, and more specifically, red ginseng, folding screen, mold opening, Baikalin, red ginseng, licorice, donkey, seaweed, sewage, horse riding and leaflet (蘇葉), characterized in that do.

The substance contained in the active ingredient according to the present invention is preferably an extract extracted from water, an organic solvent or a mixed solvent thereof, 5 to 15 parts by weight of red ginseng extract, 4 to 5 parts by weight of windproof extract, 2 to 5 parts by weight of extract Part, Baikal 0.5 to 15 parts by weight, red ginseng extract 1 to 5 parts by weight, licorice extract 0.1 to 5 parts by weight, Angelica extract 0.5 to 5 parts by weight, seaweed extract 5 to 15 parts by weight, sewage extract 0.5 to 5 parts by weight, It is preferable to contain a mixture consisting of 10 to 20 parts by weight of the horse riding extract, and 1 to 5 parts by weight of the leaflet extract as an active ingredient, and there is no need to limit the content of the active ingredient, but it can be adjusted according to the formulation and the use, but the whole It is preferable that it is 0.1-15 weight% of a composition.

Baikalin contained in the composition according to the present invention is extracted from the natural material of gold, preferably in a mixed solvent of ethanol and water, heated and concentrated under reduced pressure to prepare a primary extract, the primary extract It was prepared through the step of removing impurities by adding hexane, dissolving the primary extract from which impurities were removed in ethyl acetate, and recrystallization.

The red ginseng, windproof, ginseng, sewage and lobular extracts contained in the composition according to the present invention are each extracted from a mixed solvent of water: ethanol 1: 1.2 to 4 weight ratio, and the mold is a mixture of hexane: ethanol 1: 2 to 10 weight ratio. Extracted from the solvent, Angelica extract and horse riding extract is hot water extraction, licorice extract is extracted with ethyl acetate, seaweed extract is concentrated under reduced pressure after hot water extraction, and recrystallized in a mixed solvent of water: ethanol 1: 1.2 ~ 4 weight ratio Do.

The composition of the present invention is excellent in hair loss prevention effect, hair growth effect, wool effect, and in particular characterized in that it has a hair loss prevention and hair growth promoting effect through the activation of hair follicle cells.

In addition, the composition for preventing hair loss and promoting hair growth according to the present invention contains only components derived from natural products as an active ingredient, and has an advantage that there are no side effects due to long-term use compared to the case where the active ingredient is a chemical.

Hereinafter, each component contained in the composition of the present invention will be described in detail.

Red ginseng (Panax ginseng C. A Meyer) contained in the composition of the present invention contains glycosides, essential oils, oils, amino acids, alkaloids, carbohydrates, vitamins, resins, trace elements and the like in the root portion. Glycoside components of red ginseng include glycoside mixtures such as panakuilon, panaksik, panaxol, ginsenin, or panaxadiol-2-glucoside, and the like, and panaxadiol-3-glucoside such as panaxone and panaxin. Also exists. In particular, the red ginseng used in the present invention is ginsenoside (ginsenoside) Rg ₃ , Ginsenoside Rg ₅ , It contains a large amount of ginsenoside Rh ₂ , which enhances its effect. In particular, ginsenoside Rg ₃ enhances vasodilation and blood flow, platelet aggregation, brain nerve cell protection, and blood corticosteroids during stress. It has been reported that there is an increase inhibitory effect of the cortosterone, anticancer and immune function enhancement, and ginsenoside Rg ₅ has an inhibitory effect on platelet aggregation.

The main components of the wind-resistant ( Saposhnikovia divaricata Schiskin ) contained in the composition of the present invention contains essential oils, mannitol, high glycosides, polysaccharide oils and organic acids in the root, and α-piphenin ( About 66 chemical components are known, including α-pinene, β-pipene, β-pinene, limonine, palmitic acid and bisabolene, and coumarins. There are imperatorin, ledeboriellol, and polysaccharides include saposhikovan A, B, and C. Pharmacological action is antipyretic, analgesic, anti-inflammatory, anticonvulsant, smooth muscle relaxation, antibacterial action.

Mold opening contained in the composition of the present invention ( Schizonepeta Tenuifolia ) contains the volatile oil (Volatile oils), among which alpha-methonine (α-menthone). Pharmacological action is antibacterial action, antipyretic, analgesic action, and inhibits a number of bacteria and fungi.

Baikalin contained in the composition of the present invention is a component extracted from gold. The pharmacological action of Baikal isolated from gold is central inhibition, temperature control, blood pressure lowering, capillary strengthening, anti-arteriosclerosis, lipid metabolism improvement, hepatic injuries, dipharymia, anti-digestive ulcers , Anti-inflammatory effect, anti-inflammatory action, anti-allergic action, prostaglengin biosynthesis inhibitory action, anti-tumor action, diuretic action, kidney action, detoxification action, antiviral action, sebaceous gland Action, antibacterial action, and the like.

The ginseng ( Sophora flavescens ) contained in the composition of the present invention is the main ingredient is matrin (sofocarpidine), oxymatrine, sophoranol, anagirin, methylcytizin, babifflin, sopocarpine, allomatrine isohydro Icarridine, noranhydroicaridine, glucoluteolin, camphorol-3-sorboside, henistein, etc. in the outpost, pharmacological action is anti-allergic, diuretic, antibacterial, uterine contraction.

Licorice ( Glycyrrhiza uralensis ) contained in the composition of the present invention as a main component, flavonoids, tripene-based saponins, saponaretin, licuraside, glabridine, etc., pharmacological action detoxification, food poisoning, jaundice, cirrhosis, chronic Liver disease, rheumatism, anti-inflammatory action, myrrhosis, anti-ulcer, anti-cancer activity and so on.

Angelica gigas Nakai contained in the composition of the present invention is the component is bergaptene, isoprimpinellin, nicotinic acid, vitamin B12, n-butylidenephthalate, ligustilite, decursin, decursinol, coumarin, noda Kenerine, Umbeliferon, Nodakenin, Xanthotoxin, Isomepipinelin, Ostol, Umbelifrinin, etc. Pharmacological actions include blood pressure lowering, diuresis, sedation, protein synthesis, and cell tissue , Metabolism active, analgesic and anti-inflammatory action.

The laver contained in the composition of the present invention contains agar, hemicellulose, sorbitol, threonine, valine, leucine, isoleucine, lysine, methionine, phenylalanine, tryptophan, sodium, potassium, calcium, phosphorus, iron, carotene, vitamin A, Riboflavin, niacin, vitamin C, fucoerythrobin, dimethylsulfide, glycine, alanine and the like, pharmacological action, sedation, protein synthesis, cell activity, metabolic activity and the like.

The sewage contained in the composition of the present invention includes lecithin, crude crude fat, minerals, cinnacotoxin, polyoxypregnan ester, civicicoside D, etc., and the pharmacological action makes the hair black, pain relief, Anti-inflammatory, cell regeneration.

Riding in the composition of the present invention as a component β-sitosterol (β-sitosterol), cimicifugoside (cimicifugoside), ferulic acid (ferulic acid), cimigenol (cimigenol), methylcimicifugoside (methylcimicifugoside) And cycloatan (cycloartane) triterpene (triterpene) and the like pharmacological action is antipyretic, detoxification, intestinal, detoxification, pediatric urine, migraine tonsillitis, cell renewal metabolic activity.

The leaflets contained in the composition of the present invention (Perilla frutescens var. Acuta kudo) is the main component of L-perillaaldehyde L-limonene, alpha-pinene, dihydrocumin, cyanine chloride, parakumaric acid ester , Vitamin B1 linoleic acid, linoleic acid and the like, pharmacological action is an antiperspirant, sedative effect, diuretic effect.

Hereinafter, a method for preparing the composition of the present invention will be described.

The red ginseng, folding screen, mold opening, ginseng, licorice, donkey, seaweed, sewage, horseback riding and leaflet contained in the composition of the present invention is preferably prepared by drying each sample and then extracting hot water or organic solvent.

The red ginseng, windproof, ginseng, sewage and lobular extracts contained in the composition according to the present invention are each extracted from a mixed solvent of water: ethanol 1: 1.2 to 4 weight ratio, and the mold is a mixture of hexane: ethanol 1: 2 to 10 weight ratio. Extracted from the solvent, Angelica extract and horse riding extract is hot water extraction, licorice extract is extracted with ethyl acetate, seaweed extract is concentrated under reduced pressure after hot water extraction, and recrystallized in a mixed solvent of water: ethanol 1: 1.2 ~ 4 weight ratio Do.

Baikalin contained in the composition according to the present invention is extracted from the natural material of gold, preferably in a mixed solvent of ethanol and water, heated and concentrated under reduced pressure to prepare a primary extract, the primary extract It was prepared through the step of removing impurities by adding hexane, dissolving the primary extract from which impurities were removed in ethyl acetate, and recrystallization.

It is more preferred that glycolytic enzymes such as cellulase are added to water or a mixed solvent of water and ethanol during the preparation of the equestrian extract and bicalin and the heating temperature is maintained at 40 to 60 ° C.

After obtaining the extract of each component as described above, red ginseng 5 ~ 15 parts by weight, windproof 4 ~ 5 parts by weight, mold opening 2 ~ 5 parts by weight, Baikalin 0.5 ~ 15 parts by weight, red ginseng 1 ~ 5 parts by weight, licorice 0.1 ~ 5 It is preferable to prepare a mixture mixed by weight parts, Angelica 0.5 to 5 parts by weight, laver 5 to 15 parts by weight, sewage 0.5 to 5 parts by weight, horse riding 10 to 20 parts by weight, and leaflets 1 to 5 parts by weight. In the case of the mixing ratio, the optimum effect is obtained. When preparing a composition for preventing hair loss and promoting hair growth containing the mixture as an active ingredient, it is not necessary to limit the content of the active ingredient mixture, but preferably 0.1 to 15% by weight of the total composition.

Ingredients which may be further contained in the cosmetic composition or pharmaceutical composition for preventing hair loss and promoting hair growth according to the present invention may be applied directly to the scalp and the local surface of the hair so as to enhance the scalp and hair improvement effects. It may be used, and specifically, there are tyrosinase activators, penetration promoters, blood flow promoters, follicle inactivators or 5-reductase inhibitors, scalp nutrients, itching inhibitors and the like.

In particular, the tyrosinase activity promoter may be an extract of green tea, green tea extract, or the like. Penetration accelerator and blood flow promoting agent may be specifically iris extract, Angelica extract, witch hazel extract, Myeongmyeoncho extract, Cheongung extract, chamomile extract and the like. The blanket deactivator may be specifically clove extract, turmeric extract, sancho extract, and the like. The 5-reductase inhibitor may specifically be a fennel extract, a marigold extract, an aloe extract, a celery extract, a turmeric extract, or the like. Scalp nutrients specifically include one or more vitamins or vitamin precursors consisting of iron, calcium, manganese, essential amino acids, pyridoxine hydrochloride, biotin, vitamin B1, vitamin B2, vitamin B6, vitamin D and folic acid, menthol die-panthenol, polyquater Other than nium-7, tricetylmonium chloride, dimethicone / isolaures-6, cetyltrimethylammonium chloride, cetearyl alcohol, keratin, amino acid, cyclopentasiloxane / dimethicone, dimethicone / isolaures-6 And various minerals. The itch inhibitor may specifically be a compressor extract, panchae extract, peppermint and mixtures thereof.

The cosmetic composition for preventing hair loss and promoting hair growth of the present invention may add a base component that can be blended with general scalp and hair external preparations as needed. Specific examples include dissolution aids, surfactants, moisturizers, thickeners, pH adjusters, preservatives, antioxidants, metal ion sequestrants, fungicides, anti-inflammatory agents, antimicrobial agents, solvents, colorants, or flavoring agents. Dissolution aids may specifically be isopropyl myristate, polyethylene glycol, medium chain fatty acid triglycerides, hydrocarbons, glycols, and the like.

Among the surfactants, anionic surfactants are specifically ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium Lauryl ether sulfate (1 to 3 ethylene oxides) and the like. Among the surfactants, nonionic surfactants may specifically be polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene, cured castor oil derivatives, fatty acid diethanolamides, glyceryl stearate, and the like. The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like. Among the surfactants, the amphoteric surfactant may specifically be betaine, amidebetaine type, sulfobetaine type, steartrimonium chloride, or the like.

Moisturizing agent may be specifically glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol and the like.

The thickener may specifically be a water-soluble high molecular compound such as methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxymethyl cellulose, hydroxymethyl cellulose and the like.

The pH adjusting agent may specifically be citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid and the like.

Preservatives may specifically be benzoic acid, paraoxybenzoic acid esters, mixtures of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin and the like.

Antioxidants may specifically be dibutylhydroxytoluene, ascorbic acid and the like.

Specifically, the metal ion blocking agent may be ethylenediaminetetraacetate acetate, ethylenediaminetetraacetate tetrasodium, or the like.

Fungicides may specifically be chlorhexidine gluconate, quaternary ammonium salts, pyroxtonolamines, zinc pyrithione suspensions, iodopropynyl butylcarbamate, salicylic acid and the like.

The anti-inflammatory component may specifically be monoammonium glycyrrhinate, dipotassium glycyrizine, stearyl glycyrizinate, chamomile, alpha bisabolol, allantoin and mixtures thereof.

The antimicrobial component is specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, pyroxtonolamine, ketoconazole, arnica extract, iodopropynylbutylcarbamate, benzalkonium chloride, benzenetonium chloride, benzoic acid and salts thereof, benzyl alcohol, lavenda , Rosemary, salicylic acid, triclocarban, zinc pyrithione suspensions and mixtures thereof and the like.

The solvent may be specifically ethanol, purified water, Tween 20, cyclomethicone, mineral oil, dimethicone, and the like.

Colorants and flavoring agents can use the bases used in general scalp and hair preparations.

Hair loss prevention and hair growth promoting cosmetic composition of the present invention can be prepared in general in any formulation that can be applied to the scalp and hair, for example, shampoo, hair treatment, hair tonic, hair pack, mixed type of shampoo and rinse Can be mentioned. It is preferable to use the cosmetic composition for preventing hair loss and promoting hair growth of the present invention by a transdermal administration method such as directly applying or spraying to the scalp or hair.

Meanwhile, the pharmaceutical composition for preventing hair loss and promoting hair growth of the present invention may be prepared by further containing a pharmaceutically acceptable and physiologically acceptable adjuvant, and the adjuvant may include a solvent, a disintegrant, a sweetener, a binder, blood Solubilizers such as replication, swelling agents, lubricants, lubricants or flavoring agents can be used.

The pharmaceutical composition for preventing hair loss and promoting hair growth of the present invention may be formulated to include at least one pharmaceutically acceptable carrier, in addition to the active ingredient described above for administration.

Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, as necessary, including antioxidants, buffers, Other conventional additives such as bacteriostatic agents can be added.

In addition, the pharmaceutical composition for preventing hair loss and promoting hair growth according to the present invention may be added to a diluent, a dispersant, a surfactant, a binder, and a lubricant by adding an injectable formulation such as an aqueous solution, a suspension, an emulsion, a pill, a capsule, a granule, or a tablet. It can be formulated as.

The pharmaceutical composition for preventing hair loss and promoting hair growth of the present invention may be intravenous, intraarterial, intraperitoneal, intramuscular, intraarterial, intraperitoneal, sternum, transdermal, nasal, inhalation, topical or rectal. Administration can be in conventional manner via the oral, intraocular or intradermal route.

The amount of the composition of the present invention can be appropriately adjusted according to the individual differences, the dosage form, the form of age, the extent of the lesion, etc., preferably used in the range of about 200 to 1000 mg per day. The components contained in the composition of the present invention are components that have been clinically applied for a long time and demonstrated human stability, and do not cause side effects even when used for a long time. The composition of the present invention can be usefully used as a cosmetic, pharmaceutical, or quasi-drug for improving the scalp and hair condition.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Preparation Example 1. Preparation of Red Ginseng Extract

Red ginseng is selected from high-quality raw materials and added to the extraction tank, and extracted by pressurized heating three times using 80% by weight ethanol aqueous solution. After extraction, the resultant was filtered using filter paper and concentrated under reduced pressure to obtain a red ginseng extract.

Preparation Example 2. Windproof, Gosam, Sewage, Leaflet Extract

Windproof, red ginseng, sewage, leaflets are each added 70% by weight of ethanol aqueous solution, left for 3 days at room temperature, filtered, and concentrated under reduced pressure to prepare a windbreak, gosamchu, sewage, leaflet extract, respectively.

Preparation Example 3 Preparation of Mold Open Extract

The mold opening is added to a solvent in which 90% by weight of ethanol and 10% by weight of hexane are added, and the mixture is left to stand at room temperature for 3 days, filtered, and concentrated under reduced pressure to obtain a mold opening extract.

Preparation Example 4 Preparation of Baikal from Golden Extract

Cellulase was added to an aqueous solution containing 70% by weight of ethanol to 1% by weight, maintained at 60 ° C for 24 hours, filtered, concentrated under reduced pressure, eluted with a nucleic acid, eluted with nucleic acid to remove impurities, and then added to ethyl acetate. The eluted solution is concentrated and then recrystallized in a mixture of ethanol and water to obtain high-purity bicalin.

The structure and ¹ H-NMR spectra of the prepared baikalin is shown in Figure 1, the HPLC results of the Baikallin is shown in FIG. Figure 2 (a) is the HPLC results of known pure Baikal, (b) is the HPLC results of Baikal prepared in this Preparation Example. It was confirmed from the ¹ H-NMR and HPLC results that 92.5% purity of bicalin was obtained.

Preparation Example 5 Licorice Extract Preparation

Licorice was added to ethyl acetate, left at room temperature for 3 days, filtered and concentrated under reduced pressure to obtain a licorice extract.

Preparation Example 6 Preparation of Angelica Extract

Angelica is extracted with hot water at 80 ° C., filtered, concentrated under reduced pressure to remove moisture, and Angelica extract is obtained.

Preparation Example 7 Preparation of Seaweed Extract

The seaweed is extracted with hot water at 80 ° C., filtered, concentrated under reduced pressure to remove water, immersed in 95% alcohol, and only the crystals are recovered to obtain a seaweed extract.

Preparation Example 8 Preparation of Horse Riding Extract

Horse riding is added to 1% by weight of cellulase to purified water and then extracted with hot water at 50 ℃ filtered, concentrated under reduced pressure to remove the water and immersed in alcohol and recover only the crystals to obtain a horse riding extract.

Preparation Example 9 Preparation of an Active Ingredient Mixture According to the Present Invention

Extract of each component prepared in Preparation Examples 1 to 8 red ginseng 10 parts by weight, windproof 5 parts by weight, mold opening 3 parts by weight, Baikalin 8 parts by weight, red ginseng 3 parts by weight, licorice 3 parts by weight, Angelica 3 parts by weight, seaweed An extract mixture (JH-IJ AC extract) was prepared by mixing 8 parts by weight, 3 parts by weight of sewage, 15 parts by weight of horse riding, and 3 parts by weight of leaflets.

Example 1. Preparation of a shampoo for preventing hair loss and promoting hair growth

By mixing the raw materials as shown in Table 1 to prepare a shampoo composition for hair loss prevention and hair growth.

Raw materials Nos. 1 to 13 shown in Table 1 below were added to the main working tank, and cooled to 45 degrees after 80 ° C stirring and melting. Raw materials No. 14-18 and raw material No. 19 are added and stirred.

TABLE 1

Examples 2 and 3. Preparation of a pack for preventing hair loss and promoting hair growth

In the preparation of the hair loss and the hair improver used as the samples of the experiment shown in Table 2, each of the raw materials No. 1 to 5 and the raw materials No. 6 to 19 were put into each sub-working tank, stirred and dissolved in an 80 ° C. heating furnace, and then transferred to the main working tank. After stirring to cool uniformly to 50 ℃. Raw material No. Add 20 ~ 21 and 22 and stir to mix uniformly.

TABLE 2

Experimental Example 1. Acute toxicity test

In the preparation of the present invention, 25 ± 5g of ICR mice (Korean experimental animals) and 235 ± 10g of SPF sprag-dooli (Sprague Dawley, Biogenomics) rats were divided into 36 groups of 3 mice each. Each of the prepared red ginseng, windproof, hyunggae, ginseng, licorice, donkey, seaweed, sewage, horse riding and lobular extract, Baikal, and mixtures thereof were intraperitoneally administered at doses of 20 mg / kg, 10 mg / kg, and 1 mg / kg, respectively. The toxicity was observed for 24 hours.

As a result, no deaths were observed in all 36 groups, and no significant symptoms were found in weight gain and feed intake. Therefore, it was confirmed that the extract of the present invention is a safe drug.

Experimental Example  2. Hair Growth Experiment I

Five-week-old mice (C57BL, females) were obtained from a central experimental animal, and after approximately one week of acclimation, the general state of health was observed and only healthy animals were used for the test. Animal breeding conditions were conducted at the Chungbuk National University Laboratory Animal Research Support Center, which satisfies the KFDA conditions. The feed for mice (Samyang Feed) and water were freely provided. Animal breeding room was maintained at a temperature of 23 3 ℃, relative humidity 50 10%, 12 hours each day.

To evaluate the hair growth promoting effect, 6 week old of resting hairs in which the color of the dorsal skin is pink was used. The animal clipper was carefully trimmed with an animal clipper, 0.2 ml of sample was applied to the cut area using a brush, massaged for about 3 minutes, and rinsed thoroughly with distilled water. Sample treatment was performed once daily, and observed for 3 weeks while hairs were completely covered in the positive control group.

Samples to be treated were 10% by weight of the solvent control group (saline), 10% by weight of the extract mixture according to Preparation Example 9 (JH-IJ AC extract), 5% by weight, and 5.0% by weight of minoxidil as the positive control. Used.

Evaluation criteria were anesthetized with pentobarbital every 4 days, 7 days, 10 days and 14 days after the start of the test and photographed on the development of hair. The results are shown in FIG. 3. . The results for the hair growth were determined as described in Table 3 below, and the evaluation results are shown in Table 4 below.

TABLE 3

TABLE 4

Referring to the results of Table 4 and Figure 3, it can be seen that the composition according to the present invention is very excellent hair growth effect.

Experimental Example  3. Hair Growth Experiment II (Histological Analysis)

Two mice were autopsied at the end of the test, and the sample application site was extracted using an anatomical apparatus and fixed with formalin. After dehydration with ethanol and xylene in each step and embedded in paraffin, 5 μm sections were made using a tissue sectioner and paraffin was removed again with ethanol and xylene. Staining with hematoxylin & Eosin (H & E) was used to observe the histological changes of hair follicle tissues under an optical microscope. The results are shown in FIG. 4. Figure 4 (a) is a solvent control group, (b) the extract of Preparation Example 9 (10% by weight), (c) the extract of Preparation Example 9 (5% by weight), (d) results of minoxidil (5.0wt%) It is shown. As a result of applying the composition according to the invention it can be seen that the hair follicle cells are activated.

From the above results, it can be seen that the composition for preventing hair loss and promoting hair growth according to the present invention has superior hair follicle cell activation and hair growth promoting effects compared to a composition containing the chemical minoxidil as an active ingredient, and the composition according to the present invention. Since all of the active ingredients are derived from natural materials and do not have any side effects on long-term use, there is an advantage that they are more excellent for applying to cosmetics and the like compared to compositions containing chemicals such as minoxidil.

In the present invention, containing red ginseng, windproof, mold opening, Baikal, red ginseng, licorice, donkey, seaweed, sewage, horse riding, and lobule, hair growth effect, wool effect, excellent, prevent hair loss that does not cause side effects even in long-term use and It can be usefully applied to cosmetics as a composition for promoting hair growth.

Claims (4)

A composition for preventing hair loss and promoting hair growth, which comprises red ginseng extract, windproof extract, mold extract, Baikal, red ginseng extract, licorice extract, Angelica extract, seaweed extract, sewage extract, horse riding extract and leaflet extract. The method of claim 1, The composition of the red ginseng extract 5 ~ 15 parts by weight, windproof extract 4 ~ 5 parts by weight, mold extract 2 ~ 5 parts by weight, Baikalin 0.5 ~ 15 parts by weight, red ginseng extract 1 ~ 5 parts by weight, licorice extract 0.1 ~ 5 parts by weight Containing 0.5 to 5 parts by weight of Angelica extract, 5 to 15 parts by weight of laver extract, 0.5 to 5 parts by weight of sewage extract, 10 to 20 parts by weight of horse riding extract, and 1 to 5 parts by weight of leaflet extract. Hair loss prevention and hair growth promoting composition characterized in. The method of claim 1, Wherein the Baikal is a golden solvent in a mixed solvent of ethanol and water, heated and concentrated under reduced pressure to prepare a primary extract, the step of removing impurities by adding hexane to the primary extract, the primary extract from which impurities are removed A composition for preventing hair loss and promoting hair growth, which is prepared by dissolving and concentrating in ethyl acetate and recrystallization. The method of claim 3, wherein The red ginseng, windbreak, ginseng, sewage and leaflet extracts were each extracted from a mixed solvent of water: ethanol 1: 1.2-4 weight ratio, and the type of extract was extracted from a mixed solvent of hexane: ethanol 1: 2-10 weight ratio. , Angelica extract and horse riding extract is extracted from hot water, licorice extract is extracted with ethyl acetate, seaweed extract is concentrated under reduced pressure after extraction of hot water, water: ethanol 1: 1.2 ~ 4 weight ratio to prevent hair loss characterized in that recrystallized in a mixed solvent And a composition for promoting hair growth.

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