JP2010501551A - Use of rare earth elements for hair improvement - Google Patents

Abstract

Cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), A rare earth element selected from the group consisting of scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu), and mixtures thereof, Or the use for the hair loss prevention of the salt or oxide, hair growth promotion, hair growth, removal and prevention of dandruff, or black hair production promotion is indicated.
[Selection] Figure 5

Description

  The present invention relates to the use of rare earth elements for hair improvement, more particularly cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), Holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), Use for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair production of rare earth elements selected from the group consisting of lutetium (Lu) and mixtures thereof, or salts or oxides thereof About.

  The human body has about 100,000 to 150,000 hairs, and each hair grows and drops through a growth period (anagen), a degeneration period (catagen), and a rest period (telogen) with different periods. To do. Such a cycle is repeated over 3 to 6 years. As a result, an average of 50 to 100 hairs per day are lost. Alopecia is a phenomenon that occurs when the hair ratio in the growing period is shortened and the hair ratio in the degenerative or resting period is increased. The reason for hair loss is that male hormone androgen is excessively secreted from organs such as hair roots and sebaceous glands, and hair growth is delayed by strongly stimulating hair follicle cells to atrophy the hair follicles. It is done. Other major causes include impaired nutrient supply due to impaired local blood flow, excessive sebum secretion, peroxide, reduced scalp function due to bacteria, strong external stress, and worsening neurological symptoms due to chronic diseases. Yes. Moreover, after entering the modern society, hair loss is more serious due to overwork, environmental pollution and side effects due to misuse of addictive drugs, blood flow disturbance and inheritance of the scalp due to the use of perms, staining, mousse, spray, etc. As it becomes, interest in hair growth is increasing. Recently, not only male-type hair loss, but also obesity hair loss in women, and the tendency of hair loss in the younger layer to be diffused. Agents or hair restorers are commercially available. Commercially available hair growth agents or hair growth agents include vasodilators such as carpronium chloride and minoxidil, and hormone agents such as estrogen and estradiol that inhibit male hormone action, pentadecanoic acid and finasteride. There are male hormone activity inhibitors such as (finasteride).

  The most widely used preparations to date for the treatment and prevention of alopecia and hair growth promotion are Upjohn's minoxidil-containing preparations disclosed in US Pat. No. 4,596,812. It is one of two hair growth ingredients that have been approved by the US FDA. Minoxidil is a therapeutic agent for hypertension developed for the purpose of lowering blood pressure. However, hair growth occurred as a side effect of use, and it has become a more famous drug as a hair growth agent. Although the mechanism of the hair growth action of minoxidil has not been clarified accurately, it is thought that the growth of hair is promoted by supplying nutrients to the hair root by increasing the blood flow volume due to the vasodilatory effect. In this blood flow increase model, minoxidil expresses the expression of vascular endothelial growth factor (VEGF), a growth factor involved in vasodilation, in the dermal papilla, the main cell that makes up the hair root. It is indirectly proved by a recent report of increasing (ref. [Br. J. of Dermatol., 1998; 138; 407-411]). Moreover, in the hair growth effect mechanism of minoxidil, in addition to the vasodilatory effect, hair follicle papillary cell activation during in vitro culture (reference [Skin Pharmacol., 1996; 9; 3-8]) and hair follicle tissue culture (J. Invest. Dermatol., 1989; 92; 315-320]) suggest that minoxidil acts as a direct growth factor on hair roots . Finasteride, the main component of Merck's recently launched Propecia, is a male hormone, testosterone, which is a more powerful form of the male hormone dihydrotestosterone (DHT: dihydrotestosterone) due to the action of 5α-reductase. ) Is suppressed. In December 1997, a 1 mg tablet was approved for use by the FDA as a treatment for androgenetic alopecia. It is now on the market, and clinical results have shown that it has a significant effect (reference [J. Am Acad. Dermatol., 1998; 39; 578-589]). In addition, many attempts have been made to promote hair growth by applying or taking extracts of natural products and herbal ingredients on the scalp.

  Minoxidil and capronium chloride, which are widely used for the prevention and treatment of alopecia, have the problem that they are not excellent in clinical effects. Hormonal agents for suppressing male hormones and male hormone activity inhibitors are: Not only the clinical effect is not very high, there are problems such as side effects that suppress male function, but the active ingredient is a synthetic compound, there are safety problems in the human body, and it is sufficient for hair growth and hair growth It is difficult to obtain an effect. In addition, the composition for improving hair containing various extracts has the fact that its principle of action is not clear and its efficacy has not been sufficiently verified. The skin feels bad, such as remaining sticky, but there are problems that cause skin abnormalities. In order to overcome the limitations of hair growth agents due to side effects of chemicals, a surgical method for transplanting hair to the scalp has been proposed, but not only is it costly in the process of transplanting hair one by one, but the transplant period is also long. There is a problem in that it is merely a temporary prescription and does not become a fundamental treatment method.

  The hair is mainly composed of three parts: the epidermis, fur, and medulla, and the fur that wraps the medulla is the thickest part (80-90%) of the hair, and the melanin called melanosomes It contains granules composed of pigment, lipid membrane, TRP (Tyrosinase Related Protein) -1, TRP-2 and other proteins. Melanin pigments are synthesized in melanosomes, which are organelles in melanocytes, and migrate to the surrounding keratinocytes through the dendrites of melanocytes. Looking at the synthetic pathway of melanin, first, the amino acid tyrosine is oxidized to dopa, and tyrosinase is involved in the further oxidation to produce dopaquinone, and the subsequent reaction proceeds autoxidatively. However, it is known that enzymes such as TRP-1 and TRP-2 are involved to accelerate the reaction. The brightness of hair is determined by factors such as the type and distribution amount of eumelanin and pheomelanin formed from tyrosine, and a unique hair color appears for each person. Eumelanin is the most common and darkest pigment, forming brown and black hair, and pheomelanin is a lighter shade of color, forming a blonde. White hair is white hair formed by dopaquinone that stops its action and is keratinized, and cannot produce tyrosinase, and thus is not oxidized. White hair appears more clearly in races with dark brown hair, and is a phenomenon that occurs because melanin production stops in melanocytes, and is a kind of aging phenomenon. In addition to such senile gray hair, senile gray hair, hereditary white hair, vulgaris vulgaris, acquired white hair due to alopecia areata, white hair due to drugs and chemicals, white hair due to stress, autoimmune diseases White hair due to malnutrition, white hair due to malnutrition and nutritional imbalance, and white hair due to decreased kidney function.

  The white hair generated by a gene can be expected by gene therapy, or the white hair caused by a disease can be solved by treating the disease. In other cases, as a folk remedy, it is known how to use garlic, kinkweed, hijiki, dry yellow, maturity yellow, mulberry, plancino oil, bamboo oil, bear fat, etc. It is a difficult situation to expect.

On the other hand, rare earth elements are trace amounts of metal elements containing about 0.016% in the earth's crust, and 15 elements (La, Ce, Pr, Nd, Pm) from atomic numbers 57 to 71 in the periodic table of elements. , Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Lu) and two members of Group 3A (Sc, Y), mean a mineral containing 17 elements. Rare earth elements are in the form of salts that are ion-bonded with counterions, such as sulfate, nitrate, carbonate, acetoate, phosphate, chloride, and hydroxides. It can exist in the form of oxidized oxides. Rare earth elements have only been over 200 years since they were discovered and are not well known to the general public, but their utility value is so great that the unique electronic structure of rare earth elements makes them unique. In order to show physical and chemical performance, it is used in machinery, petrochemistry, photochemistry, etc. like permanent magnets, superconductors and fluorescent materials. Recently, it is used in various fields such as agriculture, forestry, and livestock industry. But application is being tried. As a biological effect, rare earth elements promote photosynthetic action and chlorophyll formation in higher plants, promote bud germination, root vitality, activation of respiratory action, promote nutrient transfer, water regulation, cells Delaying the senescence process of the leaf, showing functions such as promoting division, promoting transfer of hormone transfer, promoting absorption of nutrient elements and transport, activation of substance metabolism, increased RNA and protein synthesis in the leaf, Increases protein content of green algae, promotes photosynthesis and oxygen release activity, promotes protein and chlorophyll formation. La ³⁺ is known to enhance the function of Ca ²⁺ ions in the body by increasing the activity of (Na ⁺ , K ⁺ ) -ATP enzyme and Mg ²⁺ -ATP enzyme in human erythrocyte cell membrane (References) [The Journal of Biological Chemistry; 1986; 261 (20); 9552-9557]), some rare earth elements have an antibacterial effect (Chem. Pharm. Bull .; 2003; 51 (5); 494-498], which suppresses the production of active oxygen and exhibits antioxidant activity (Literature [Biochemical and Biophysical Research Communication; 2006. 2; 342; 86-91]), and some rare earth elements carry alkaline ions. It is known that it has the effect of suppressing fatigue and aging phenomenon by the action of far infrared rays, the cell activity in the body, the promotion of blood circulation, the enhancement of metabolism, the increase of tissue regeneration ability, Increase and feed conversion rate (reference [J. Anim. Physiol. A. Anim. Nutr .; 2001; 85; 263-270]) In addition, rare earth elements belong to low-toxic substances and are hardly absorbed through the digestive tract and have no special internal accumulation phenomenon, leading to malformations, mutations, or cancer. It was revealed that there was no inducing effect (Reference [Environmental Health Perspectives; 1996; 104; 85-95]).

  Korean Patent No. 569,083 is a metal oxide formed by dissolving cerium oxide with a metal ion having an ionic radius larger than a tetravalent cerium ion and / or a metal ion having a lower valence than the tetravalent cerium ion. An ultraviolet blocking agent containing solid solution cerium oxide and a cosmetic containing the same are disclosed, and it is described that this can also be used as a hair cosmetic. However, this relates to a metal ion solid solution of cerium oxide, which is completely different from the use of rare earth elements, their salts or mixed rare earth. US Pat. No. 5,112,360 discloses hair dyes containing 5,6-dihydroxyindole and / or indole derivatives and at least one rare earth element salt. However, this is related to the hair dyeing effect of a composition comprising an indole compound and a rare earth element salt. When applied to hair roots and hair, the rare earth element alone has a direct black hair production promoting effect. There is no suggestion. Japanese Unexamined Patent Publication No. 2003-137749 (2003.14.14) discloses a natural radioactive rare earth such as lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd) or lutetium (Lu). Natural ore containing elements is differentiated to a particle size of 5 μm or less, and this is mixed with a coloring agent and mixed material mixed in an alcoholic solvent and a viscous binder made of a solute soluble in this into a spray container. A hair thickener contained in a spray container is disclosed. The published patent relates to a hair thickening or false hair thickening agent for treating hair defects and improving the environment near the scalp while making it appear as if the hair has been visually increased, and promoting the regeneration or growth of the own hair. Thus, after spraying, it is described that β-rays released by radioactive rare earth elements attached to the scalp form negative ions and promote blood circulation, thereby showing the effect of promoting self-renewal or hair growth. However, the above-mentioned published patent relates to the blood circulation promoting action by β-ray emission of natural radioactive rare earth element alone and the expected hair growth or hair growth effect. The promotion of cell growth and the suppression of TGF-β expression by the rare earth element. No mention is made of promotion of VEGF expression, tyrosinase activity and expression, or promotion of melanin production.

  In consideration of various problems of existing hair growth agents, the present inventors are excellent in hair loss prevention, hair growth promotion, hair growth, dandruff removal and prevention, black hair generation, which is safe to the human body and causes no side effects. Sustained research has been conducted to develop accelerators. As a result, it was surprisingly confirmed that one or more rare earth elements can be effectively used for the above-mentioned purposes, and the present invention has been completed.

  Accordingly, a first object of the present invention is to provide a composition for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair generation, which contains one or more rare earth elements.

  The second object of the present invention is to provide a use for preventing hair loss of one or more rare earth elements, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair production.

  The third object of the present invention is to provide a method for preventing hair loss, promoting hair growth, growing hair, removing and preventing dandruff, or promoting black hair generation using one or more rare earth elements.

  The fourth object of the present invention is to provide a method for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or producing a black hair production promoter using one or more rare earth elements.

  According to the first aspect of the present invention, as an active ingredient, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er) ), Thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof The present invention relates to a composition for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair production, comprising a rare earth element selected from the group consisting of: or a salt or oxide thereof.

  The second aspect of the present invention includes cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium ( Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof The present invention relates to a use of a rare earth element selected from the group, or a salt or oxide thereof for preventing hair loss, promoting hair growth, growing hair, removing and preventing dandruff, or promoting black hair production.

  The third aspect of the present invention provides cerium (Ce), praseodymium (Pr), promethium (Pm), and europium for subjects that require hair loss prevention, hair growth promotion, hair growth, dandruff removal and prevention, or black hair generation promotion. (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium Hair loss prevention comprising applying a rare earth element selected from the group consisting of (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof, or a salt or oxide thereof, The present invention relates to a method for promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair generation.

  The fourth aspect of the present invention includes cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium ( Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof The present invention relates to a method for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or producing a black hair production promoter using a rare earth element selected from the group, or a salt or oxide thereof.

  Hereinafter, the present invention will be described in detail.

  As used herein, the term 'anti-hair loss' refers to the prevention, inhibition, inhibition and reduction of partial or complete hair loss.

  The term 'promoting hair growth' as used in the present invention is defined as the meaning encompassing the maintenance, induction, stimulation, promotion and regeneration of hair development, the growth of defective hair, or the extension of the growth phase in the hair cycle.

  The term 'hair growth' used in the present invention is defined as a comprehensive meaning that prevents fur from falling off, promotes growth / growth, or converts fur into terminal hair.

  In the present invention, 'hair loss' or 'hair loss' means loss of hair growth and partial or total hair loss, androgenic alopecia or androgenetic alopecia, toxic alopecia, alopecia areata , Resting alopecia, endocrine abnormalities, metabolic disorders, nutritional disorders, drug-induced alopecia, mechanical alopecia, alopecia associated with skin diseases, scarring alopecia, congenital alopecia, tricotyromania, etc. Including but not limited to all.

Since rare earth elements share similar physicochemical properties, there is no particular limitation on the type of rare earth elements that can be used in the present invention, and any one of the rare earth elements can be used alone or as a mixture with other rare earth elements. It is more preferable to use cerium (Ce). Rare earth element salts can be used as long as they are non-toxic, pharmaceutically or cosmetically acceptable, and examples thereof include sulfates, nitrates, carbonates, acetoates, Examples thereof include, but are not limited to, phosphates and chlorides. Similarly, oxides of rare earth elements (for example, Ce ₂ O ₃ , La ₂ O _{3 and the} like) can be used without limitation as long as they are non-toxic pharmaceutically or cosmetically acceptable. In the present invention, the rare earth element oxide is a concept including a rare earth element hydroxide (eg, Ce (OH) ₃ , La (OH) _3, etc.).

  In the present invention, the rare earth element can be used by being pulverized into a fine powder, and is contained as 0.0001 to 10% by weight, preferably 0.001 to 10% by weight, more preferably 0.001 to 0.1% by weight. Is done.

  The composition of the present invention may further contain a pharmaceutically or cosmetically acceptable carrier. The compositions of the present invention are commonly used in the pharmaceutical or cosmetic manufacturing field or can be prepared using any of the well-known devices and methods (see [Remington's Pharmaceutical Science, 15th Edition). , 1975, Mack Publishing Company, Easton, Pennsylvania 18042 (Chapter 87: Balug, Seymour)]). The composition of the present invention usually contains a base, and if necessary, components generally used as chemicals or cosmetic raw materials can be appropriately blended. As the base of the composition of the present invention, any commonly used one can be used, and examples thereof include purified water, mineral water, ethanol, glycerin, squalene, 1,3-propylene glycol, 1, Contains 3-butylene glycol, coconut oil, camellia oil, and liquid petrolatum. Examples of other components blended in the composition of the present invention include, but are not limited to, surfactants, emulsifiers, thickeners, preservatives, antioxidants, and perfumes.

  The surfactant is a cationic surfactant, an anionic surfactant or a nonionic surfactant. Examples of cationic surfactants are alkali salts of higher fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and oleic acid (eg sodium salt, potassium salt, ammonium salt, triethanolamine salt); lauryl sulfate Alkyl sulfate salts such as sodium and lauryl sulfate triethanolamine; and alkyl ether sulfate salts such as polyoxyethylene lauryl ether sodium sulfate and polyoxyethylene lauryl ether sulfate triethanolamine. Examples of nonionic surfactants include polyoxyethylene alkyl ethers such as polyoxyethylene lauryl ether and polyoxyethylene oleyl ether; and alkanolamides such as coconut oil fatty acid diethanolamide and lauric acid diethanolamide. Examples of anionic surfactants include stearyl trimethyl ammonium chloride, cetyl trimethyl ammonium chloride and stearyl bis (diethyl alcohol) hydroxyethyl ammonium chloride.

  Examples of emulsifiers include cetanol, stearyl alcohol and behenyl alcohol.

  Examples of thickeners include sodium alginate, methyl cellulose, carboxymethyl cellulose, polyvinyl alcohol and polyvinyl pyrrolidone.

  Examples of preservatives include ethyl parahydroxybenzoate, butyl parahydroxybenzoate and benzalkonium chloride.

  Examples of antioxidants include butylhydroxytoluene, propyl gallate and butylhydroxyanisole.

  Perfumes are perfumes that are mainly used for general purposes, examples of which include citrus, lavender and floral.

In addition to the above components, the composition of the present invention includes monosaccharides such as glucose, xylose, mannose, and arabinose that serve to supply nutrients to the hair follicles, and disaccharides such as maltose, sucrose, cellobiose, and trehalose. One or more saccharides can be further included, and these amounts are preferably 0.01 to 1% by weight of the total composition. In addition, the composition of the present invention can contain commonly used hair growth promoting auxiliary components, such as carrots, bitter ginsengs, mandarin oranges, green tea, carrots, bellflowers, mulberry coconuts, tanuki beans, stone husks. , Matsuba, Tsukimiko, Konotegasiwa leaves, what neck moth, moth lotus grass, tribulus extract, betaine, mint extract, nettle extract, horsetail extract, lavender extract, hop extract, aloe, elegans, winter moth summer grass, tokkuri Strawberry, Mulberry, Wood vinegar, Bamboo salt, Yellow flowers, Yellowtail, Eggplant, Walnut, Grape, Saflower, Sesame, Sesame, Garlic, Kelp, Wakame, Plum, Mung bean, Black rice, Pentadecanoic acid glyceride, Hinokitiol, Pepper tincture, ginger tincture, cantharis tincture, forskolin, trans-3,4'-dimethyl-3-hydroxyflavanone, a Tocopherols such as tocopherol cetate, nicotinic acid, nicotinamide, retinol palmitate, β-carotene, calciferol, folic acid, biotin, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, calcium pantothenate, pantothenyl ethyl ether , Ascorbic acid, grapefruit seed extract, cephalanthin, nicotinic acid benzyl ester, vitamins A, B ₁ , B ₂ , B ₆ and derivatives thereof, pantothenic acid and derivatives thereof, minoxidil, purple oil extract, arginine, aspartic acid, Methionine, serine, glycine, glutamic acid, cystine, amino acid extract, β-glycyrrhetinic acid, glycyrrhizic acid, pyridoxine hydrochloride, salicylic acid, allantoin, photosensitizer 301, isop Pills methylphenol, piroctone olamine, glycerin, pyrrolidone carbon acid, estradiol, ethinyl estradiol, and 1 one or more components selected from a group consisting of menthol may further contain. These amounts are preferably 0.0001 to 10% by weight of the total composition.

  On the other hand, an excellent TGF-β due to the synergistic action between the two components when the rare earth element of lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd) or lutetium (Lu) and chili tincture are combined. Not only hair loss prevention, hair growth promotion, hair growth, dandruff removal and prevention effect by expression reduction and VEGF expression promotion, but also black hair production promotion effect by tyrosinase activity and expression promotion and melanin production promotion can be achieved. The blending ratio of the two components is not particularly limited as long as the desired synergistic effect is achieved. For example, the rare earth element and chili pepper tincture are in the range of 1: 0.0001 to 1, preferably 1: 0.01 to 1. More preferably, it is contained in a weight ratio of 1: 0.1-1. This is because the desired synergistic effect can be maximized within the above range, but the scope of the present invention is not limited to the specific range. Furthermore, the composition can further contain ginger tincture and / or cantharis tincture. Ginger tincture is a tincture obtained by leaching the rhizome of ginger (Zingiber officinate roscoe) with ethanol, and stimulating ingredients such as zingerone and shogaol stimulate hair roots to promote hair growth. It is known. Cantharis refers to the insects belonging to the family Nymphalidae, and cantalis tincture is a component obtained by ethanol extraction from the insects.

  There is no restriction | limiting in particular in the application object of the composition of this invention, The composition of this invention is applicable not only to a human but all animals including a domestic animal and a pet.

  The composition can be administered transdermally or orally, for this purpose lotions, ointments, gels, creams, patches, sprays, tablets, pills, powders, sachets, elixirs ), Suspensions, emulsions, solutions, syrups or soft or hard capsules.

  In particular, the composition of the present invention can be used by a method such as direct application or application to the hair or scalp. The hair to which the composition of the present invention is applied includes all parts of the hair roots and hair follicles, hair and eyebrows and eyelashes, whiskers, eyelashes, pubic hair, hair roots and hair follicles of the entire body. Therefore, the present invention includes hair nick, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair pack, hair treatment, eyebrow hair growth agent, eyelash hair growth agent or eyelashes Can be used in nutrients or pet shampoos and pet rinses.

The capacity of the rare earth element according to the present invention can be appropriately determined in consideration of sex, age, hair loss symptoms, hair condition and the like. Usually, the daily dose for adults is about 0.1-5 mg / cm ² and can be applied 1-5 times a day.

It is the graph which showed the cell growth promotion effect by the density | concentration of each component. It is the graph which showed the cell growth promotion effect of mixed rare earth and each rare earth element in fixed density | concentration. It is a photograph which shows the hair growth promotion effect of the composition by this invention in an animal. It is the photograph which observed the animal skin tissue with the microscope after the process of the composition by this invention. FIG. 4 is a graph showing TGF-β2 and VEGF expression in animal tissues after treatment of the composition according to the present invention. It is a photograph which shows the hair growth promotion effect of the composition by this invention in a human. It is the graph which showed the tyrosinase activity after processing each element to a human origin melanoma cell strain according to a density | concentration. It is the graph which showed the tyrosinase activity after processing each element to a mouse | mouth origin melanoma cell line according to a density | concentration. It is the graph which showed tyrosinase expression after processing mixed rare earth and each rare earth element to a human origin melanoma cell line and a mouse origin melanoma cell line. It is the graph which showed the melanin production amount after processing mixed rare earth and each rare earth element to a human origin melanoma cell line and a mouse origin melanoma cell line. It is the graph which showed the expression level of TGF- (beta) 2 and VEGF after mix | blending and processing a pepper tincture in a lantern. It is the graph which showed the melanin production amount, after mix | blending a capsicum tincture with a lantern and processing in a human and mouse origin melanoma cell line.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, the scope of the present invention is not limited to these Examples.

<Example 1: Production of composition for improving hair>
Using the components and contents shown in Table 1, the mixed rare earth powder and other components were uniformly mixed, and then filtered to produce a hair improving composition. The produced hair improving composition was light brown, opaque, and had a pH of 5.0 to 8.5.

<Examples 2 to 18: Production of hair improving composition>
In Example 1, instead of mixed rare earth, Ce (Example 2), Pr (Example 3), Pm (Example 4), Eu (Example 5), Tb (Example 6), Dy ( Example 7), Ho (Example 8), Er (Example 9), Tm (Example 10), Yb (Example 11), Sc (Example 12), Y (Example 13), La (Example) Example 14), substantially the same except that individual rare earth elements of Nd (Example 15), Sm (Example 16), Gd (Example 17) and Lu (Example 18) were used respectively. The composition for improving hair was prepared through various processes.

<Example 19: Production of hair treatment composition>
In Example 1, except that 0.1% by weight of mixed powder of La, Nd, Sm, Gd and Lu was used instead of mixed rare earth powder, and 0.01% by weight of chili pepper tincture was further used. In this way, a hair improving composition was produced by substantially the same process. The prepared hair treatment composition was light brown, opaque and had a pH of 5.0 to 8.5.

<Examples 20 to 24: Production of hair treatment composition>
In Example 19, instead of the mixed powder of La, Nd, Sm, Gd and Lu, La (Example 20), Nd (Example 21), Sm (Example 22), Gd (Example 23) and A hair treatment composition was prepared by substantially the same process except that each of the rare earth elements of Lu (Example 24) was used.

<Comparative example>
A composition was prepared by removing the rare earth powder from the composition of Example 1.

<Experimental Example 1: Measurement of Cell Growth Promotion Effect by Rare Earth Elements>
NIH 3T3 cells, which are mouse embryo fibroblasts, were 2 × 10 ⁶ or 4 × 10 ⁶ cells / ml in DMEM (Dulbeceo's Modified Eagle's Medium) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Was maintained in a constant temperature / humidity incubator at 37 ° C. (95% O _2, 5% CO _2, 60% humidity). The MTT (3- (4,5-dimethyl-thiazol-2-yl) -2,5-diphenyltetrazoliumbromide) assay method was used for cell growth promotion experiments. For the MTT experiment, 2 × 10 ³ cells / ml of each cell were sorted into a 96-well plate and a culture solution containing 10% FBS was treated. After stabilizing the cells for 24 hours in an incubator, the culture medium containing FBS is replaced with DMEM without FBS, and mixed rare earth of 10 μg, 1 μg, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg and 1 pg, respectively. , And 10 ng of individual rare earth elements. After incubation for a certain period of time, 50 μl of MTT (2 mg / 1 ml sterilized distilled water) was added per well, reacted for 4 hours, treated with DMSO (dimethyl sulfoxide), shaken for 30 minutes, and then an ELISA reader (550, Bio-rad). ) Was measured at 540 nm.

  FIG. 1 shows the result of cell growth depending on the concentration of each element (mixed rare earth, Ce, Eu, La, Gd), and FIG. 2 shows the result of treatment of each element at a constant concentration. As shown in FIG. 1, the experimental group treated with each element from 10 μg to 1 pg could confirm that the cell proliferation was more active than the untreated group where nothing was treated. In particular, cell growth increased 1.69 times after addition of cerium compared to the untreated group. Therefore, it was confirmed that each element has an effect on cell proliferation. In addition, as shown in FIG. 2, when each element was treated with 10 ng, cell proliferation increased compared to the untreated group, and cerium (Ce), praseodymium (Pr), promethium (Pm), europium ( Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), etc. are approximately equal to each other. It was confirmed to show an effect. In particular, when treated with cerium (Ce), it was confirmed that the cell proliferation was greatly increased. Compared to this, it was confirmed that lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and the like have a relatively inferior effect.

<Experimental Example 2: Measurement of hair growth effect in animals>
The experimental animals are 6-week-old C57BL6 male mice (29 ± 3 g). Before starting the experiment, the animals were allowed to freely take a general chow (Samyang) and water, and a polypropylene box (453 × 293 × 247 (H) mm, 19 l). ) For 1 week (20 to 24 ° C., humidity 60 to 80%). Thereafter, a fixed area (3 × 5 cm ² ) of the back part of the experimental animal group was cut, and the composition treatment group of the comparative example (control group) and the composition treatment group of Example 1 and Example 2 were randomly selected. Divided into composition treatment groups (experimental group). Each group was maintained for 24 hours a day, divided into light and dark states for 12 hours each. A fixed amount (1 ml) of the composition prepared according to Examples 1 and 2 and Comparative Example was applied twice a day at a fixed time in the morning and night. In all experiments, the experiment was not performed in the dark state, and consideration was given to the stability of the experimental mice, and a recovery period of 24 hours before the experiment was allowed when collecting the tissues.

  The results are shown in FIG. As shown in FIG. 3, when treated with the compositions of Example 1 and Example 2, it was confirmed that hair grew over time. When treated with the compositions of Example 1 and Example 2, the speed of hair growth was significantly faster than when treated with the composition of the comparative example, and there was a large difference in the number of hairs. I was able to confirm that I was winning. Further, after 20 days from the composition treatment, the hair growth rate was very fast, and a clear difference between the experimental group and the control group could be confirmed. In particular, the composition of Example 2 to which cerium was added showed almost the same effect as the composition of Example 1 to which mixed rare earth was added.

<Experimental Example 3: Observation of animal skin tissue>
After sacrificing the experimental animal of Experimental Example 2 by cervical dislocation, the skin was separated, foreign matter was removed to the maximum with PBS (Phosphate buffered saline), the experimental site was cut out with scissors, and used for the following experiment did. The separated skin is placed in 10% formaldehyde, fixed for one day, cut into 10 mm ³ size, and automatically infiltrated with an automatic permeator for 12 hours. Then, a paraffin block is made and 4 μm is used using a slicer. Sliced to a thickness of. Then, it dye | stained by hematoxylin & eosin staining conditions. Interpretation and photography were performed at 100x using an optical microscope.

  The results are shown in FIG. As shown in FIG. 4, it was found that the experimental group treated with the compositions of Example 1 and Example 2 and the control group treated with the comparative composition had a large difference in hair growth. In the experimental group, hair follicles originate from the skin and adipose tissue and the dermis and grow and grow on the epidermis side, whereas in the control group, the growth starts. It turned out to be a stage. It was confirmed that the number of hair follicles was higher in the experimental group than in the control group. In particular, the composition of Example 2 to which cerium was added showed almost the same effect as the composition of Example 1 to which mixed rare earth was added. For reference, the experimental animal is not a mosaic type having a different hair cycle like a human being, but a synchronous type in which the hair cycle of the whole hair matches.

<Experimental Example 4: Measurement of TGF-β2 and VEGF expression>
TGF-β is an allogeneic and regulates early development, cell cycle regulation, cell proliferation and differentiation, migration, survival, extracellular matrix production, immune system, blood vessel generation in various cells and tissues Is a multifunctional cytokine that regulates the production of blood cells, cell death induction, skeletal formation, wound healing, etc., and TGF-β is expressed and hair cell death occurs during the degeneration phase of the hair cycle It is known to induce. Vascular epidermal growth factor (VEGF) is an allogeneic glycoprotein, the most common and effective growth factor known to stimulate angiogenesis at the wound site, expressed in many vascular tissues, and epithelial It is known that mitogen specific to cells induces migration and proliferation of epithelial cells and increases vascular permeability. VEGF is a hair growth mechanism of minoxidil that is already on the market. It is thought that hair follicles, which are the main cells that make up the hair roots, supply hair roots by increasing blood flow due to vasodilatory effects and promote hair growth. Yes.

  In order to confirm the expression level of the above protein by Western blotting, the protein obtained from the experimental animal tissue of Experimental Example 3 was separated by 12% SDS-PAGE and then transferred to NC (nitrocellulose membrane). At this time, in Examples 2 and 3, a group treated with the composition of Example 14 to which lanthanum was added was included. NCs were then blocked with Tris-buffered saline (TBS) containing 5% skimmed milk and 1: 1 with primary antibodies (anti-TGF-β2 and anti-VEGF rabbit IgG) diluted 1: 1,000. Each was reacted with a secondary antibody diluted to 1,000,000 (peroxidase-conjugated anti-rabbit IgG), then washed with TBS containing 0.05% twin-20, and using an ECL detection kit. X-ray film was exposed. Thereafter, the OD values were displayed graphically using an image analysis program of LabWork ™ (version 4.5.00.0).

  The results are shown in FIG. As shown in FIG. 5, in the composition-treated group (experimental group) of Examples 1, 2, and 14, the expression level of TGF-β2 was larger than that in the comparative composition-treated group (control group). It turns out that it decreases. It was also confirmed that VEGF was expressed more in the experimental group than in the control group. That is, the composition for improving hair of the present invention decreases the activity of TGF-β and promotes the activity of VEGF, thereby suppressing the death of hair follicle cells by TGF-β and the blood flow volume due to the vasodilation effect. By increasing the nutrients to the hair roots and promoting hair growth, hair loss can be prevented and hair growth can be achieved. In particular, in the composition treatment group of Example 2 containing cerium, an effect superior to that of the composition treatment group of Example 1 containing mixed rare earth appeared. On the other hand, in the composition-treated group of Example 14 containing lanthanum, an effect superior to that of the untreated group appeared, but the effect relatively inferior to the composition-treated groups of Example 1 and Example 2. Appeared.

<Experimental Example 5: Measurement of hair growth and black hair effect in human>
Thirty male and female patients with various alopecia were treated with the composition of Example 1, the composition of Example 2, and the composition of Comparative Example, respectively, and then their effectiveness was evaluated. The scalp was administered twice a day for 2 months every day, and the hair growth state was evaluated after 1 month and 2 months. Judgment criteria are
1. Highly effective-new hair is observed (hard hair)
2. Intermediate effect-new hair is observed (soft hair)
3. 3. Has a little effect-reduces the number of hair loss The results are shown in Table 2.

  As shown in Table 2 above, in the composition according to the present invention, the hair growth and hair growth effects appear in 1 month of treatment and are maintained even in the 2nd month. In addition, after applying the compositions of Example 1 and Example 2, the effect of reducing hair loss appears within 1-2 weeks, and there are individual differences, but after applying the composition, within 5-7 days The effect of reducing dandruff appeared, and as time passed, the dandruff was completely removed and the head itch was completely eliminated. In the case of males and females with white hair among the subjects, it was confirmed with the naked eye that black hair was generated. As can be seen from the above results, in the hair growth time and the hair growth effect, the composition of the present invention is not greatly different among individuals, is effective for hair loss patients, and increases the thickness of the hair in the entire subject, Promoted black hair generation. In addition, no side effects were found. When treated with the composition of the comparative example, fluff grew, hair loss decreased, dandruff removed, etc., but no significance was seen.

  Among the test subjects, 40- to 47-year-old males were photographed before and 2 months after application of the composition of Example 2 and are shown in FIG. As shown in FIG. 6, the test subject began to lose hair, had almost no hair in the frontal area or the top of the brain, and began to show signs of baldness. Symptoms have been severe hair loss due to head itchiness, dandruff, fatigue due to stress, and the like. Therefore, as a result of applying the composition of Example 2 to the scalp twice a day, itching and dandruff in the head disappeared 3 days after application, and the hair loss phenomenon was remarkably reduced. A hair like a fluff started to grow on the hair removal site of the hair, and the hair started to grow continuously, and it became a hair state that could be confirmed with the naked eye after two months of application. From the above results, it was confirmed that the composition according to the present invention not only has an excellent hair growth effect, but is also harmless to the human body even when applied for a long period of time.

<Experimental Example 6: Measurement of tyrosinase activity for evaluating the effect of promoting black hair production>
In order to evaluate the effect of promoting black hair production, the activity of tyrosinase was examined in Sk-mel-31 which is a human melanoma cell and B16F10 which is a mouse melanoma cell. For cell culture, in RPMI-1640 (1% penicillin-streptomycin, 10% fetal bovine serum) medium, Sk-mel-31 had a density of 1 × 10 ⁶ / ml and B16F10 had a density of 2 × 10 ⁵ / ml. While maintaining, the cells were cultured in a constant temperature / humidity incubator at 37 ° C. (5% CO _2, 60% relative humidity). After stabilizing the cells for 24 hours in an incubator, the culture medium containing FBS is replaced with RPMI-1640 without FBS, and mixed with 10 μg, 1 μg, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg and 1 pg, respectively. Rare earth and 10 ng of individual rare earth elements were treated. In order to measure tyrosinase activity, after culturing for a certain period of time, the cells were collected by centrifugation (100 × g) for 10 minutes, and then 0.01 M sodium phosphate buffer (1% Triton X-100, 0.1 mM). Cells were ultrasonically disrupted with PMSF (phenylmethylsulfonyl fluoride), pH 7.4), left on ice for 1 hour, and then centrifuged at 40,000 × g for 20 minutes at 4 ° C., and only the supernatant was collected. 100 mM potassium phosphate (pH 7.1), 4% N, N-dimethylformamide, 0.1% (v / v) Triton X-100 mixed with 250 μl of analytical buffer was mixed with 5 mM L-DOPA (L-3 , 4-dihydroxyphenylalanine) 130 μl, 25 mM MBTH (3-methyl-2-benzothiazolinone hydrazone) 120 μl was added and reacted in a 37 ° C. thermostat for 5 minutes. 100 μg of cell extract was added and reacted again for 2 hours. Thereafter, 500 μl of 1M perchloric acid was added, and centrifuged at 9,000 × g for 3 minutes at room temperature. Thereafter, only the supernatant was taken and the absorbance was measured at 505 nm.

  FIGS. 7 and 8 show the results when sk-mel-31 and B16F10 were treated with mixed rare earth and individual rare earth elements (Ce, Eu, La, Gd), respectively. As shown in FIGS. 7 and 8, in the experimental group treated with mixed rare earth and individual rare earth elements from 10 μg to 1 pg, it is confirmed that the tyrosinase activity is increased as compared with the control group not treated with the mixed rare earth. I was able to. Therefore, it was confirmed that the rare earth element affects tyrosinase activity. That is, it was found that the mixed rare earth and the individual rare earth elements increase the enzyme activity of tyrosinase and promote melanin production, and thus have a black hair production promoting effect. On the other hand, when treated with individual rare earth elements, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er) It has been confirmed that thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), and the like can exhibit substantially the same effect as the mixed rare earth. In particular, when treated with cerium (Ce), it was confirmed that the enzyme activity of tyrosinase was greatly increased. On the other hand, it was confirmed that lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and the like are somewhat less effective than the above elements.

<Experimental example 7: Tyrosinase expression measurement for evaluating the effect of promoting black hair production>
After culturing the cells by the same method as in Experimental Example 6, the cells were collected by centrifugation (100 × g) for 10 minutes. To confirm the expression level of tyrosinase by Western blotting, 100 μg of cell extract was separated by 10% SDS-PAGE, transferred to NC, blocked with TBS containing 5% skim milk, and then 1: 2, A primary antibody diluted to 500 (monoclonal anti-tyrosine hydroxylase clone Th-2) and a secondary antibody diluted to 1: 5,000 (Anti-mouse IgG) were reacted to give 0.2% twin-20. It was washed with the contained TBS and exposed to X-ray film using an ECL detection kit. Thereafter, the OD values were displayed graphically using an image analysis program of LabWork ™ (version 4.5.00.0).

  The results are shown in FIG. As shown in FIG. 9, it was confirmed that the expression of tyrosinase increased when the mixed rare earth was treated as compared with the control group. Therefore, it was found that the mixed rare earth increases the expression of tyrosinase and promotes the production of melanin pigment that determines hair color. On the other hand, when treated with individual rare earth elements, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er) It has been confirmed that thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y) and the like have substantially the same effect as the mixed rare earth. In particular, when treated with cerium (Ce), it was confirmed that the expression of tyrosinase was greatly increased. On the other hand, it was confirmed that lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and the like are somewhat inferior to the above elements.

<Experimental Example 8: Measurement of melanin production for evaluating the effect of promoting black hair production>
After culturing the cells by the same method as in Experimental Example 6, the cells were harvested by centrifugation (100 × g) for 10 minutes. In order to measure the amount of melanin produced, 200 μg (total 1 ml) of cell extract was added to 1N NaOH, left at room temperature for 30 minutes, and the absorbance was measured at 400 nm.

  The results are shown in FIG. As shown in FIG. 10, when mixed rare earth was processed, it turned out that the production amount of melanin increases compared with a control group. From these results, it was found that the amount of melanin increased due to increased activity and expression of tyrosinase, and the effect of promoting black hair production was achieved. On the other hand, it was confirmed that substantially the same effect can be obtained when treated with individual rare earth elements as when treated with mixed rare earth. On the other hand, when treated with individual rare earth elements, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er) It was confirmed that thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), and the like have substantially the same effects as described above. In particular, when treated with cerium (Ce), it was confirmed that the amount of melanin was greatly increased. On the other hand, it was confirmed that lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and the like are somewhat inferior to the above elements.

<Experimental Example 9: Comparison of TGF-β and VEGF expression by lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and chili pepper tincture, and melanin production amount>
In order to investigate the synergistic effect of the combination of lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd) and lutetium (Lu) and chili tincture among rare earth elements, the expression levels of TGF-β and VEGF, And melanin production was compared.

  In order to examine the expression levels of TGF-β2 and VEGF, cells were cultured by the same method as in Experimental Example 1, and then the degree of expression was confirmed by the same method as in Experimental Example 4.

  For the melanin production amount experiment, cells were cultured and harvested by the same method as in Experimental Example 6, and then the melanin production amount was measured by the same method as in Experimental Example 8.

  The results are shown in FIG. 11 and FIG. As shown in FIG. 11, in the case of TGF-β2, when the composition of Example 20 containing lanthanum and chili pepper was processed, it was similar to the case of processing the composition of Example 2 containing cerium. Decreased expression level. In the case of VEGF, when the composition of Example 20 containing lanthanum and chili tincture was treated, the increase in the expression level was similar to that when the composition of Example 2 containing cerium was treated. Also, as shown in FIG. 12, when the composition of Example 20 containing lanthanum and chili pepper was processed, the amount of melanin produced was similar to the case of processing the composition of Example 2 containing cerium. Showed an increase. On the other hand, when the compositions of Examples 21 to 24 in which neodymium (Nd), samarium (Sm), gadolinium (Gd), and lutetium (Lu) and chili tincture were mixed were processed, it was substantially the same as the above. It was confirmed that an advantageous effect was obtained.

  As described above, the composition for improving hair according to the present invention reduces the expression of TGF-β, prevents the death of hair follicle cells, promotes the expression of VEGF, and increases blood flow due to vasodilation. By supplying nutrients to the hair root and promoting hair growth, it not only has excellent hair loss prevention, hair growth promotion, hair growth, dandruff removal and prevention effects, but also activates melanocytes around the hair root to promote melanin By increasing the production of pigments, it has the effect of promoting black hair generation, is harmless and safe for the human body, has high skin absorbability, is non-sticky, has excellent skin feel, and has no side effects on the scalp.

Claims (24)

  As active ingredients, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium Selected from the group consisting of (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof. A composition for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair production, comprising a rare earth element or a salt or oxide thereof.   The composition according to claim 1, comprising cerium (Ce), or a salt or oxide thereof.   The composition according to claim 1, comprising 0.0001 to 10% by weight of a rare earth element, or a salt or oxide thereof. Carrot, bitter, mandarin orange peel, green tea, carrot, bellflower, mulberry coconut, bamboo peas, stone husk, pine needles, moonlight, Konotegasiwa leaves, what neck moths, moth lotus grass, tribulus extract, betaine, mint extract, nettle Extract, Toxa extract, Lavender extract, Hop extract, Aloe, Ezokogi, Winter strawberry summer grass, Tokuri strawberry, Mulberry, Kiso sap, Bamboo salt, Yellow flower twilight, Onitake, Eggplant, Walnut, Grape, Saflower , Sesame, sesame, garlic, kelp, wakame, plum, mung bean, black rice, pentadecanoic acid glyceride, hinokitiol, pepper tincture, ginger tincture, cantalis tincture, forskolin, trans-3,4'-dimethyl-3-hydroxyflavanone, Tocopherols, nicotinic acid, nicotinamide, retinol palmitate, β-carotene, calci Ferrol, folic acid, biotin, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, calcium pantothenate, pantothenyl ethyl ether, ascorbic acid, grapefruit seed extract, cephalanthin, nicotinic acid benzyl ester, vitamin A, B ₁ , B ₂ , B ₆ and derivatives thereof, pantothenic acid and derivatives thereof, minoxidil, mussels extract, arginine, aspartic acid, methionine, serine, glycine, glutamic acid, cystine, amino acid extract, β-glycyrrhetinic acid, glycyrrhizic acid, pyridoxine hydrochloride , Salicylic acid, allantoin, photosensitizer 301, isopropylmethylphenol, piroctone olamine, glycerin, pyrrolidone carbon acid, estradiol, ethinyl estradiol, Characterized in that it contains further one or more hair growth promoting auxiliary component selected from a group consisting of beauty 1-menthol composition according to claim 1.   A rare earth element selected from the group consisting of lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu) and mixtures thereof, or a salt or oxide thereof, and chili pepper tincture The composition according to claim 4, comprising:   The composition according to claim 5, further comprising at least one of ginger tincture and cantharis tincture.   The composition according to claim 1, further comprising a pharmaceutically or cosmetically acceptable carrier or additive.   That the pharmaceutically or cosmetically acceptable carrier or additive is selected from the group consisting of surfactants, emulsifiers, thickeners, preservatives, antioxidants and perfumes, and mixtures thereof. 8. Composition according to claim 7, characterized in that   The composition according to claim 7, wherein the composition is administered by transdermal or oral administration.   Formulated into lotions, ointments, gels, creams, patches, sprays, tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups or soft or hard capsules The composition according to claim 9, wherein   Hair nick, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair pack, hair treatment, eyebrow hair growth agent, eyelash growth agent or eyelash nutrition, or for pets 2. Composition according to claim 1, characterized in that it is used in shampoos and pet rinses.   2. Composition according to claim 1, characterized in that it is for application to humans, domestic animals or pets.   Cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), A rare earth element selected from the group consisting of scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu), and mixtures thereof, Or a salt or oxide for preventing hair loss, promoting hair growth, hair growth, removing and preventing dandruff, or promoting black hair production.   14. Use according to claim 13, characterized in that the rare earth element is cerium (Ce) or a salt or oxide thereof. Rare earth elements, or their salts or oxides, ginseng, bitter, mandarin orange, green tea, carrot, bellflower, mulberry coconut, bamboo bean, stone husk, pine needles, moonflower, Konotegasiwa leaves, garlic lotus, moth lotus, tribulus Extract, betaine, mint extract, nettle extract, laceweed extract, lavender extract, hop extract, aloe, weevil, winter strawberry summer grass, red snapper strawberry, mulberry, wood vinegar, bamboo salt, yellow flower tuna, onitake, Eggplant, walnut, grape, safflower, sesame, egoma, garlic, kelp, seaweed, plum, mung bean, black rice, pentadecanoic acid glyceride, hinokitiol, chili tincture, ginger tincture, cantalis tincture, forskolin, trans-3, 4 '-Dimethyl-3-hydroxyflavanone, tocopherols, nicotinic acid, nicotinamide, pal Retinol mymate, β-carotene, calciferol, folic acid, biotin, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, calcium pantothenate, pantothenyl ethyl ether, ascorbic acid, grapefruit seed extract, cephalanthin, benzyl nicotinate Ester, Vitamin A, B ₁ , B ₂ , B ₆ and derivatives thereof, Pantothenic acid and derivatives thereof, Minoxidil, Murasakixembry extract, Arginine, Aspartic acid, Methionine, Serine, Glycine, Glutamic acid, Cystine, Amino acid extract, β- Glycyrrhetinic acid, glycyrrhizic acids, pyridoxine hydrochloride, salicylic acid, allantoin, photosensitizer 301, isopropylmethylphenol, piroctone olamine, glycerin, pyrrolidone carbon acid, Stra diol, ethinyl estradiol, and 1 one or more hair growth promoting auxiliary component selected from a group consisting of menthol and blended characterized in that it uses, applications of claim 13.   A rare earth element selected from the group consisting of lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu), and mixtures thereof, or a salt or oxide thereof, The use according to claim 15, wherein the use is formulated.   The use according to claim 16, wherein one or more of ginger tincture and cantharis tincture is further blended and used.   For subjects requiring hair loss prevention, hair growth promotion, hair growth, dandruff removal and prevention, or black hair production promotion, cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb) Dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm) Applying a rare earth element selected from the group consisting of gadolinium (Gd), lutetium (Lu) and mixtures thereof, or a salt or oxide thereof, preventing hair loss, promoting hair growth, hair growth, dandruff Removal and prevention, or black hair production promotion method.   The method according to claim 18, wherein cerium (Ce) or a salt or oxide thereof is applied. Rare earth elements, or their salts or oxides, ginseng, bitter, mandarin orange, green tea, carrot, bellflower, mulberry coconut, bamboo bean, stone husk, pine needles, moonflower, Konotegasiwa leaves, garlic lotus, moth lotus, tribulus Extract, betaine, mint extract, nettle extract, laceweed extract, lavender extract, hop extract, aloe, weevil, winter strawberry summer grass, red snapper strawberry, mulberry, wood vinegar, bamboo salt, yellow flower tuna, onitake, Eggplant, walnut, grape, safflower, sesame, egoma, garlic, kelp, seaweed, plum, mung bean, black rice, pentadecanoic acid glyceride, hinokitiol, chili tincture, ginger tincture, cantalis tincture, forskolin, trans-3, 4 '-Dimethyl-3-hydroxyflavanone, tocopherols, nicotinic acid, nicotinamide, pal Retinol mymate, β-carotene, calciferol, folic acid, biotin, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, calcium pantothenate, pantothenyl ethyl ether, ascorbic acid, grapefruit seed extract, cephalanthin, benzyl nicotinate Ester, Vitamin A, B ₁ , B ₂ , B ₆ and derivatives thereof, Pantothenic acid and derivatives thereof, Minoxidil, Murasakixembry extract, Arginine, Aspartic acid, Methionine, Serine, Glycine, Glutamic acid, Cystine, Amino acid extract, β- Glycyrrhetinic acid, glycyrrhizic acids, pyridoxine hydrochloride, salicylic acid, allantoin, photosensitizer 301, isopropylmethylphenol, piroctone olamine, glycerin, pyrrolidone carbon acid, Stra diol, characterized by ethinyl estradiol and 1-menthol configured one or more hair growth promoting selected from the group from the auxiliary component and blended applying method of claim 18.   One or more rare earth elements selected from the group consisting of lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu), and mixtures thereof, or a salt or oxide thereof The method according to claim 20, wherein the method is applied in combination with chili pepper tincture.   The method according to claim 21, wherein one or more of ginger tincture and cantharis tincture is further blended and applied.   The method according to claim 18, which is applied to transdermal or oral administration.   Cerium (Ce), praseodymium (Pr), promethium (Pm), europium (Eu), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), A rare earth element selected from the group consisting of scandium (Sc), yttrium (Y), lanthanum (La), neodymium (Nd), samarium (Sm), gadolinium (Gd), lutetium (Lu), and mixtures thereof, Alternatively, a method for producing a hair loss prevention agent, hair growth promotion, hair growth, hair growth, dandruff removal and prevention, or black hair production promoter using the salt or oxide thereof.