KR101547758B1 - Composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and stimulating hair growth, more specifically, to a composition containing nicotinoyl tripeptide, biotinoyl tripeptide, cinnamon extract, meadowsweet extract, Epilobium fleischeri extract, Papenfussiella kuromo extract, and wintergreen extract as active ingredients. According to the present invention, the composition: promotes proliferation of dermal papilla cells and keratinocytes; shows excellent antioxidant effects; causes no side effects such as skin irritation if applied to a scalp; and is configured to be excellent in preventing hair loss and stimulate hair growth. Hence, the composition is expected to prevent hair loss and stimulate hair growth without side effects if applied to products such as hair restorers, external skin applications, cosmetics, and cleansers.

Description

Technical Field [0001] The present invention relates to a composition for preventing hair loss and for promoting hair growth and hair growth,

The present invention relates to a composition for preventing hair loss and for promoting hair and hair growth, and more particularly, to a composition for promoting hair loss and hair growth, which comprises nicotinoyl tripeptide, biotinoyl tripeptide, broiler chick extract, A hair extract, and a wintergreen extract as an active ingredient, and a composition for promoting hair loss and hair growth.

The hair grows with a constant cycle and produces different types of hair in the same hair follicle. It does not grow for a lifetime like a nail but grows for a certain period of time and has a hair cycle of hair loss again and growing again (hair cycle) . This cycle of hair is four to seven years for women and three to five years for men. The average rate of hair growth is about 0.35mm / day, which varies depending on the location, age and sex of the hair follicles and grows about 1 to 1.5cm per month. The number of hairs in general people is between 8 and 120,000, and it is normal to lose about 40 ~ 100 strands per day on average. The growth rate of hair is faster in the day than in the day, and in spring and summer in winter. At any point, 85 to 90% of hair corresponds to growing hair, and the growth period is between 3 and 7 years.

In the growing phase, the hair grows continuously. In the stromal cells in the mollusk, the base of the hair follicle, cell division starts rapidly. These cells form the three hair layers, the hair follicle and the epidermal layer, and produce keratin, a strong and elastic protein that dies during the process of moving toward the scalp surface. Growth phase can be divided into three stages again divided into proanagen, mesanagen and metanagen. In the proanagen phase of the growing stage, the growth starts with the synthesis of the hair follicle RNA and DNA, and proceeds to the next step, mesanagen and metanagen. The hair follicle size is maximized at the stage of hair production. However, in the period corresponding to the retrograde period of hair, hair follicle growth activity is abruptly stopped and atrophied, and the hair follicle is shortened by dividing the follicle with the follicle, and the follicle begins to move upward. The dermal papillae shrink, the hair follicles shrivel, the hair follicle upwards, the follicle deeper, the hair follicle completely stopping, starting to fall from the base of the hair follicle, and hair loss begin to occur. The papilla, which had been resting during the resting period, merges with the mossy part, and cell division occurs again to greet the new growth period of the hair. As the new hair grows, the aging hair of the resting paw is pushed out and dropped. The most important thing in the hair growth cycle is the interaction between the epidermis and mesenchymal tissue. Structurally, there is limited contact between the dermal papillae and the epidermis in the retrogressive or dormant period, but in the growing phase the hair epithelium encircles the dermal papillae, resulting in more active influence from the dummy papillae. There are many factors involved in the hair growth cycle, such as structural proteins, enzyme adhesins, growth regulators, transcription regulators, extracellular substances, and substances involved in cell death, all of which are associated with hair loss. The causes of hair loss were summarized as follows. First, as westernized diet increases meat consumption, excessive intake of high protein, high calorie animal fat or protein increases metabolic disease, which causes poor circulation of nutrients to the mother's pelvic floor. As a result, Is accelerated. In addition, the harmful ingredient added to the instant food accumulates in the body, adversely affecting the cell degeneration of the viscera and causing deformation to the hair. Second, it is the most serious cause of stress. Stress is involved in autonomic nerves and hormones. Tension and anxiety are secreted by the endocrine system in the adrenal gland, the sympathomimetic stimulant, epinephrine, which increases heart rate and respiration, increases blood pressure, and releases stored glucose from cells. And reacts. Eventually, hair growth is adversely affected. This stress also affects digestive organs, reducing digestive power, increasing cholesterol levels, and causing blood circulation disorders, resulting in malnutrition of the papilla. In addition, the lack of sleep, blood flow and oxygen, nutrient supply to the scalp is not enough to reduce the body temperature and nutrition can not be continued if the possibility of hair loss is high, the use of chemical drugs or drugs, or long- Repeated exposure to strong ultraviolet rays can cause hair loss and hair loss.

Heretofore hair growth (US Pat. No. 4,139,619) using 6- (1-piperidinyl) -2,4-phenylimidinediamine-3-oxide (minoxidil) as an active ingredient, amino acids (US Patent No. 5,538,945) and short sequence GPIGS peptide (Biol. Pharm. Bull. 28 (3) 485-489, 2005) (Korean Patent No. 10-1045438) Korean Patent No. 10-0770798 discloses wool and hair growth effect of a complex natural product. These hair growth promoters, hair restorers, and hair loss inhibitors have the effect of converting the resting period of hair into a growth phase and extending the growing period of hair. The process of hair growth and degeneration is very complicated and may be caused by one or two causes, but many factors are combined and various factors are linked to each other. Synthetic peptide of physiologically active function restores the function of hair in a short period of time. However, it is not excellent in promoting hair growth as a sole ingredient, and is not excellent in preventing hair loss in all of natural extracts (SI Kang, JO Lee, Research on patent trends of hair loss prevention and hair regrowth, J. Beauty Art Society, 3 (2), 2009).

Accordingly, the present inventors have made extensive efforts to develop new cosmetics that are effective in preventing alopecia, preventing hair loss, and promoting hair growth. As a result, it has been found that nicotinoyl tripeptide, biotinoyl tripeptide, broiler chick extract, Meadowweed extract, The present invention has been accomplished based on the findings that the composition containing Kerry extract, Chrysanthemum morifolium extract, and Wintergreen extract exhibits excellent alopecia prevention, hair loss prevention and hair growth promoting effect without any side effects such as skin irritation and the like.

United States Patent No. 4,139,619 United States Patent No. 5,538,945 Korean Patent No. 10-1045438 Korean Patent No. 10-0770798

S. I. Kang, J. O. Lee, Research on patent trends of hair loss prevention and hair regrowth, J. Beauty Art Society, 3 (2), (2009).

Accordingly, it is a main object of the present invention to provide a composition excellent in alopecia prevention, hair loss prevention and hair growth promoting effect.

It is another object of the present invention to provide an agent for preventing alopecia using the composition or a cosmetic for preventing hair loss and promoting hair growth.

According to one aspect of the present invention, there is provided a pharmaceutical composition comprising a nicotinoyl tripeptide represented by the following formula (1), a biotinoyl tripeptide represented by the following formula (2), a broiler chick extract, , Epilobium flavor extract, epilobium extract, wintergreen extract and an extract of Echilobium flavonacea as an active ingredient, and a cosmetic composition for promoting hair growth and hair growth.

[Chemical Formula 1]

(2)

The nicotinoyl tripeptide used in the present invention is an essential nutrient for hair growth. The nicotinoyl tripeptide is an essential nutrient for hair growth. It is an essential nutrient for niacin (vitamin B3) to prevent aging of scalp, enhance nerve function, (GHK) tripeptide. It is free of cytotoxicity, has excellent cell growth and cleavage promoting function, and is safe for human body.

The biotinoyl tripeptide used in the present invention is a biotin (C ₁₀ H ₁₆ N ₂ O ₃ S) conjugated with a tripeptide of Gly-His-Lys (GHK), and functions as a hair bulb And increases blood circulation of the papilla, thereby reducing hair loss and showing hair growth effect.

The nicotinoyl tripeptide of Formula 1 and the biotinol tripeptide of Formula 2 may be prepared according to known methods or may be purchased commercially.

Broiler (meat) refers to the bark of a broiler tree (scientific name: Cinnamomum Loureirii or Cinnamomum Cassia Blume), originating in China and cultivated in Korea, Indochina and Laos. It is sweating due to the onion, has a good fragrance, is flavorful and irritating. It promotes blood circulation, increases leukocyte count, has excellent antibacterial effect and protects skin cells. In addition, excellent sebum secretion control function makes the scalp clean. In the present invention, it is preferable to use broth obtained by extraction with 50 to 100% (v / v) ethanol. More preferably, 0.5 to 2 L of 50 to 100% (v / v) ethanol is added per 100 g of broiler chickens, and the mixture is extracted at room temperature (10 to 30 ° C) for 5 to 10 days. At this time, it is preferable to conduct the concentration under reduced pressure to completely remove the solvent to obtain a powder state. In the present invention, an extract having an extraction yield of 7 to 15% based on the weight of broiler chickens is preferably used.

Meadow Sweet (Filipendula Ulmaria) is a perennial plant of rosewood rosacea, native to southern Europe and the Mediterranean, and contains a large amount of salicylic acid in its blossoms. 5 5-alpha reductase It excellently inhibits type 2 enzyme and has antibacterial and anti-seborrheic effect and skin astringent effect. It contains a large amount of catechinic tannin and thus has excellent cell activity. In the present invention, it is preferable to use Meadow Sweet Extract obtained by using a mixed solution of butylene glycol and water as a solvent. At this time, Meadow Suites can use outposts. More preferably, an aqueous solution of 40 to 60% (v / v) butylene glycol in an amount of 5 to 10 times the dry weight of the meadow sweets is added and the mixture is extracted at 40 to 95 ° C for 4 to 20 hours. It is good to use it. At this time, it is preferable that the decompression concentration allows the solvent to be removed as much as possible. In the present invention, it is preferable to use an extract having an extraction yield of 1 to 5% based on the weight of mallow juice.

Willowherb (Scientific name: Epilobium Fleischeri) is a perennial herbaceous plumaceae, and is known as an alpine alpine plant. The efficacy of the extract is excellent in the ability to control sebum and is highly inhibitory of IL-8 and IL-1a. Especially, it has excellent effect of inhibiting 5-alpha reductase enzyme and has excellent anti-inflammatory effect. The Efilobium Plaque Kerry Extract has excellent antioxidant properties and prevents damage to skin cells and provides enough nutrients to the scalp and hair follicles to make the scalp and hair healthy. In the present invention, it is preferable to use Ethyl Bee Place Kerry extracted with a mixed solution of glycerin and water as a solvent. At this time, Efilobium Place Kerry can use outposts. More preferably, it is preferable to use an aqueous solution of glycerin in an amount of 40 to 60% (v / v) 5- to 10-fold by weight of the dry weight of epilobium perchelia, followed by extraction at 40 to 95 ° C for 4 to 20 hours. It is good to use it. At this time, it is preferable that the decompression concentration allows the solvent to be removed as much as possible. In the present invention, it is preferable to use an extract having an extraction yield of 3 to 8% based on the weight of epilobium flavonacea.

Papenfussiella Kuromo (Yendo) Inagaki is a kind of papaya, distributed mainly in Japan, and in Korea, it grows in Samcheok, Kisung, Kuryongpo, Ulleungdo, Mokdo, Busan, Yasugi Island, Geomundo and Heuksan Island. It grows up to 50cm in length and has dark brown long hair on the whole surface. It has excellent antioxidant effect and anti-inflammatory effect to prevent scalp inflammation, and it has an excellent antibacterial effect and plays an important role in cell physiological activity. Regulate sebum secretion to normalize scalp function. In the present invention, it is preferable to use a mixture of 50 to 100% (v / v) ethanol extracted from the flaxseed. At this time, we can use outpost. It is more preferable to use 0.5 to 2 L of 50 to 100% (v / v) ethanol per 100 g of flaxseed flesh and extract at room temperature (10 to 30 ° C) for 5 to 10 days, . At this time, it is preferable to conduct the concentration under reduced pressure to completely remove the solvent to obtain a powder state. In the present invention, it is preferable to use an extract having an extraction yield of 5 to 11% based on the weight of the artificial hair.

Wintergreen (Scientific name: Gaultheria Procumbens) is a species of evergreen perennial herb that lives in cold regions and is also known as a presser foot. It is also a component of natural salicylic acid. It is a small blue shrub that is native to Canada. It grows in coniferous forests, bushes and vacant land in North America. Wintergreen has straight branches and leaves alternate with oval, rough, shiny and jagged corners. Native Americans dyed the leaves of this plant to calm headaches, fever, and relieve the redness of the respiratory tract. Wintergreen is a powerful anti-inflammatory drug because it contains a rich methyl salicylate. This material is also used as an analgesic with features that inhibit cyclooxygenase, prostaglandin synthesis, and bradykinin release. Wintergreen leaves contain phenolic compounds such as arbution and various phenolic compounds such as ferulic acid, p-hydrobenzoic acid, p-coumaric acid, caffeic acid, gallic acid, vanillic acid, salicylic acid, syringic acid, protocatechuic acid and gentisic acids . Wintergrain's powerful penetrating effect normalizes scalp function and helps hair growth. In the present invention, it is preferable to use wintergrain extracted with 50 to 100% (v / v) ethanol. At this time, it is better to use leaves for wintergreen. More preferably, 50 to 100% (v / v) ethanol of 5 to 10 times the weight of the dry weight of the wintergreen is added and extracted at 40 to 95 ° C for 4 to 20 hours, . At this time, it is preferable that the decompression concentration allows the solvent to be removed as much as possible. In the present invention, it is preferable to use an extract having an extraction yield of 10 to 30% based on the weight of epilobium flavonacea.

Generally, the peptide component does not exhibit the effect by the reducing power of the active group but exhibits a specific function by the sequence of the amino acid, and therefore, the peptide component is excellent in stability. In addition, since it is a biomaterial, it exhibits fast metabolism and thus has excellent physiological activity. And because the plant extracts are natural products that have been used for a long time by humankind, they have little side effects and are safe for human body. Since the composition of the present invention is composed of these components, it has an advantage that it can exert an excellent effect safely and stably on the human body.

The composition of the present invention can be prepared by mixing each of the above materials in an appropriate amount. According to the present invention, 0.001 to 5% by weight of nicotinoyl tripeptide, 0.001 to 5% by weight of biotinoyl tripeptide, 0.1 to 10% by weight of broiler chick extract, 0.1 to 10% It is preferable that the extract is prepared so as to contain 0.1-10% by weight of the extract, 0.1-5% by weight of the ephibrobium extract, 1-10% by weight of the extract of the foxtail, and 0.1-3% by weight of the extract of wintergreen. More preferably, 0.05 to 2% by weight of nicotinoyl tripeptide, 0.05 to 2% by weight of biotinoyl tripeptide, 1 to 3% by weight of broiler chick extract, 1 to 5% by weight of Meadowoo sweet extract, It is preferable that the amount of the non-dried place-making kerri extract is 0.5 to 2% by weight, the concentration of the sea tangle hair extract is 5 to 7% by weight, and the content of the wintergreen extract is 0.5 to 2% by weight.

The content of each of the above materials is based on the content showing the highest effect as confirmed by the experiment. If the content exceeds the proper amount, the stability of the formulation may occur, and if the content is insufficient, the effect may be significantly reduced.

The composition of the present invention can be used as a hair cosmetic such as a hair cleanser, a hair setting agent, and a skin external preparation for hair loss prevention and hair growth promotion since each component constituting the composition is safe for human body or skin. An auxiliary component such as a hair nutrient component, an antioxidant, an anti-sebum inhibitor, an anti-dandruff agent, and a moisturizer may be added according to circumstances such as enhancement of a hair growth promoting effect function of the composition and a user's constitution. Examples of the components of the hair nutrients, anti-dandruff agent, moisturizing agent and the like include collagen, keratin, and iodopropynyl butylcarbamate. These auxiliary components can be used in such a range as to maintain the formulations without interfering with the effect of the main component, and can be preferably added in an amount of 20 to 90% by weight based on the total weight of the composition.

Formulations of the external preparation for skin may have formulations such as lotions, emulsions, gels, ointments, creams, liquids, aerosols and the like.

When it is used as a cosmetic material, a material arbitrarily selected for the purpose of formulation or use may be added. For example, purified water, an oil component, a surfactant, a moisturizer, a higher fatty acid, a higher alcohol, a thickener, a chelating agent, a pigment, an antioxidant, an antiseptic, a wax, a pH adjusting agent and a perfume may be added.

Examples of the oil component include silicone oil, avocado oil, cetyl ethylhexanoate, butyleneglycolic dicaprylate / dicaprate, macadamia seed oil, mineral oil, apricot seed eucalyptus oil, isopropyl myristate, emu oil, Mink oil, sunflower seed oil, almond oil, green tea seed oil, and the like.

Examples of the surfactant include surfactants such as cetearyl olivate, sorbitan olivate, polysorbate 80, glyceryl stearate, hydrogenated phosphatidylcholine, polysorbate 20, phage-100 stearate, Cocamide deia, and the like.

Examples of the moisturizing agent include sodium hyaluronate, glycerin, sorbitol, dipropylene glycol, butylene glycol, pullulan, betaine and the like.

Examples of the higher alcohol include cetostearyl alcohol, cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol, and the like. Examples of the thickening agent include hydroxyethylcellulose, sodium acrylate / sodium acryloyldimethyltaurate copolymer, ammonium polyacryloyldimethyltaurate / Vpicopolymer, carbomer, xanthan gum, glyceryl acrylate / acrylic acid Copolymers, polyquaternium-10, acrylate copolymers, hydroxypropyl guar hydroxypropyl trimonium chloride, and the like.

As the chelating agent, disodium dithiophosphate, tetrasodium dithiophosphate and the like may be used.

The coloring matters may include black 401, blue 1, blue 102, red 106, yellow 4, yellow 5, and the like.

Examples of the fatty acid include lauric acid, stearic acid, myristic acid, palmitic acid, isostearic acid, and the like.

Examples of the antioxidant include ascorbic acid, tocopheryl acetate, butylated hydroxytoluene, and the like.

Examples of the preservative include methylparaben, butylparaben, propylparaben, phenoxyethanol, and the like.

Examples of the wax include ceresin, jojoba wax, microcrystalline wax, ozokerite, carnauba wax, candelilla wax, beeswax, and wax.

Examples of the pH adjuster include sodium hydroxide, potassium hydroxide, diethanolamine, monoethanolamine, triethanolamine, sodium citrate, citric acid, and the like.

As the fragrance, natural fragrance such as rosemary, lavender, chamomile, synthetic fragrance and the like may be blended.

The formulations of the present invention are not particularly limited, but may be formulations selected from any form of formulation that can be applied to the scalp and hair, such as hair tonics, hair treatments, shampoos, rinses, etc., and may be applied directly to the scalp or hair Spraying or the like.

The amount of the composition of the present invention can be adjusted depending on the formulations and the condition of the subject to be applied, and is not limited. However, it is expected that 0.2 to 2.0 g per day can be applied continuously or intermittently on the basis of an adult subject.

According to the present invention, when the composition of the present invention promotes cell proliferation of dermal papilla cells and keratinocytes and exhibits excellent antioxidative effects and prevents side effects such as skin irritation when applied to scalp, superior hair loss prevention and hair growth promoting effect It can be expected to prevent or prevent hair loss and promote hair growth without side effects when applied to a hair loss prevention agent, external preparation for skin, a cosmetic preparation and a cleaning agent.

1 is a graph showing the effect of the composition of the present invention on human dermal papilla cells and keratinocyte cell proliferation. A is a human dermal papilla cell, and B is a result of keratinocytes.
2 is a graph showing the antioxidant activity of the composition of the present invention measured.
FIG. 3 is a photograph of a result of a clinical experiment showing an effect of preventing hair loss and promoting hair growth of a hair tonic containing the composition of the present invention. A is the hair tonic of the present invention, and B is the result of the comparative example.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1. Preparation of composition

The composition was prepared according to the composition of Table 1 below.

Raw material name Content (% by weight) Nicotinoyl tripeptide 0.50 Biotinoyl tripeptide 0.50 Broiler extract 5.00 Meadow Sweet Extract 3.00 Epilobium Placecurry Extract 3.00 Chestnut hair extract 7.00 Wintergreen extract 0.50 Methylparaben 0.20 ethanol 3.00 Polysorbate 80 1.50 Purified water Suitable amount

The nicotinoyl tripeptide and the biotinoyl tripeptide were synthesized as shown in Formulas 1 and 2, respectively. The extracts of Meadow's Sweet Extract (Silab, France), Ethyl Bee Placecurry Extract (manufactured by Pentapharm, Switzerland) , And wintergreen extract (manufacturer: Alban Muller International, USA) were purchased and used.

At this time, the Meadow's Sweet Extract was extracted by using a butylene glycol solution as a solvent. An aqueous solution of 40-60% (v / v) butylene glycol in a weight of 5-10 times weight was added to the dried Meadow Sweet Outpost, In a distillation apparatus equipped with a distillation apparatus at 40 to 95 ° C for 4 to 20 hours, filtering it with a filter cloth, and concentrating it under reduced pressure. About 2.7% in semi-solid form (based on the weight of the vehicle used in the extraction).

The ephilophyllum cepa extract is obtained by using the above Meadowweed extract extraction method, but using glycerin instead of butylene glycol. About 5.1% in the semi-solid form (based on the weight of the epilubic free saccharide used in the extraction).

The wintergreen extract is a powder prepared by using the above method of extracting Meadowweed extract and using about 95% (v / v) ethanol as a solvent. And has an extraction yield of about 20%.

Broiler chick extract and chondroitin extract were prepared as follows.

Broiler extract

The broiler was washed with running water three times or more to remove impurities, completely dried, and pulverized by a pulverizer to obtain a pulverized product of about 300 mesh. To 100 g of the obtained pulverized product was added 1 liter of a 94% ethanol aqueous solution (purified water), and the mixture was extracted with stirring at room temperature for 7 days. The above procedure was repeated three times. The obtained extracts were combined and concentrated under reduced pressure at 40 ° C to obtain 11.2 g of an extract of concentrated solid form (extraction yield: 11.2%).

Chestnut hair extract

8.3 g of an extract in the form of a solid was obtained (extraction yield: 8.3%) in the same manner as the chicken broth extract, except that soybean flour was used instead of broiler chick.

Experimental Example 1. Confirmation of Growth Effect of Cells by Composition Treatment

The human hair dermal papilla cells used in this experiment were purchased from ScienceCell Research Laboratory (USA), and keratinocytes were purchased from ATCC (USA). Each cell is 10% FBS (Gibco BRL, Grand Island, NY, USA) and 1% antibiotics (Gibco BRL) is added to a Dulbecco's Modified Eagle's Medium (DMEM ; Gibco BRL) , using the medium 37 ℃, 5% CO ₂ conditions Lt; / RTI > To confirm cell proliferation effect, WST assay was performed using Cell Counting Kit (Dojindo, Japan).

Human dermal papilla cells and keratinocytes were seeded at 1.5 × 10 ⁴ cells / well in a 96-well plate and cultured for 12 hours. Then, the mixed samples were treated at concentrations of 0, 10, 50, 100, 500 and 1,000 ppm And cultured for 24 hours. 10 μl of WST-8 solution was added to each well and reacted at 37 ° C and 5% CO ₂ for 3 hours. An ELISA reader (BIO-TEK Instruments Inc., Power wave X340, Winooski, Absorbance at 450 nm. The experimental values were expressed as percentages of the control group in which the samples were not treated, and are shown in Fig. 1 as human dermal papilla cells (A) and keratinocytes (B).

Experimental Example 2. Measurement of DPPH radical scavenging ability

Excessive oxidative stress can convert the growing hair into a dormant period, which can cause various hair conditions and cause hair loss. Therefore, the higher the antioxidant capacity, the more protective the hair dermal papilla cells can prevent hair loss.

The method of Kilani et al. (Kilani et al., 2004) was used to confirm the DPPH radical scavenging ability of the composition of the present invention. 100 μl of samples at various concentrations (10, 50, 100, 500 and 1,000 ppm) and 100 μl of 0.2 mM DPPH solution were mixed well and reacted at room temperature for 10 minutes. Absorbance was measured at 517 nm using an ELISA reader, Respectively. The measured values were expressed as electron donating ability as follows.

Electron donating ability (%) = (1-absorbance of sample addition group / absorbance of no addition group) x 100

Statistical analysis

The quantitative results obtained in this experiment are expressed as mean value and standard deviation. Statistical analysis was performed using Student's t-test.

Example 2. Hair tonic manufacture

Using the composition of Example 1, a hair tonic was prepared as shown in Table 2 below.

Each raw material (% by weight) was weighed, agitated with an Agi Mixer at 100 rpm for 10 minutes, and then filtered.

Mixing order Purpose of blending Raw material name Example 2 Comparative Example One solvent Purified water Suitable amount Suitable amount 2 solvent ethanol 5.00 5.00 3 Moisturizer Butylene glycol 2.00 2.00 4 Moisturizer glycerin 1.00 1.00 5 Chelating agent Disodium iodide 0.02 0.02 6 Hair nutrition The composition of Example 1 30.00 - 7 antiseptic Phenoxyethanol 0.20 0.2 8 antiseptic Methylparaben 0.10 0.10 9 Surfactants Polysorbate 80 0.50 0.50 10 Flavoring agent Spices Suitable amount Suitable amount

Unit: wt%

Experimental Example 3. Clinical Experimental Evaluation

Example 3 The effect of preventing hair loss of hair tonic, wool and hair growth was evaluated.

Hair tonic was applied once / day to the hair loss region of a male patient in his forty years old who had alopecia, and the scalp state was measured after 3 months, and is shown in FIG.

EXPERIMENTAL EXAMPLE 4 Evaluation of Clinical Experiment According to Composition of Composition

The hair tonic was prepared in the composition as shown in Table 3 by varying the components of the composition. The hair condition of the patient was compared at intervals of 15 days after applying the hair once per day to the hair loss region of 40 male patients of 40 or more having alopecia. The evaluated average values are shown in Tables 4 to 6.

The evaluation criteria are as follows.

Eight specimens (agar-agar) × 5 samples per sample = 40 samples (5 samples per sample)

<Post-treatment evaluation status: evaluation score>

- Excellent after-treatment improvement: 4.0

- Excellent effect: 3.0 ~ less than 4.0

- slight effect: 2.0 to less than 3.0

- No progress or minimal effect: 1.0 to less than 2.0

- worse: 0

Purpose of blending Raw material name Example Comparative Example end I All la hemp bar four Ah One solvent Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 2 solvent ethanol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 3 Moisturizer Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 4 Moisturizer glycerin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 5 Chelating agent Disodium iodide 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02



6



sample Nicotinoyl
Tripeptide 0.15 - 0.15 0.15 0.15 0.15 0.15 0.15 Biotinol
Tripeptide 0.15 0.15 - 0.15 0.15 0.15 0.15 0.15 Broiler extract 1.50 1.50 1.50 - 1.50 1.50 1.50 1.50 Meadow Sweet Extract 0.90 0.90 0.90 0.90 - 0.90 0.90 0.90 Epilobium Placecurry Extract 0.90 0.90 0.90 0.90 0.90 - 0.90 0.90 Chestnut hair extract 2.10 2.10 2.10 2.10 2.10 2.10 - 2.10 Wintergreen extract 0.15 0.15 0.15 0.15 0.15 0.15 0.15 - ethanol 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.90 Polysorbate 80 0.45 0.45 0.45 0.45 0.45 0.45 0.45 0.45 7 antiseptic Phenoxyethanol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 8 antiseptic Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 9 Surfactants Polysorbate 80 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 10 Flavoring agent Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Unit: wt%

division 15 days past 30 days past end I All la hemp bar four Ah end I All la hemp bar four Ah hair loss
Prevention 1.6 1.1 1.2 1.5 1.3 1.4 1.5 1.6 2.0 1.3 1.3 1.3 1.4 1.8 2.0 2.0 wool
effect 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.5 1.3 1.5 1.5 1.4 1.3 1.4 1.3 Hair growth
effect 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.5 1.3 1.3 1.6 1.4 1.5 1.3 1.3 Hair growth
effect 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.4 1.0 1.0 1.1 1.2 1.1 1.2 1.3

division 45 days past 60 days past end I All la hemp bar four Ah end I All la hemp bar four Ah hair loss
Prevention 3.1 1.7 1.8 1.9 2.0 2.1 2.7 2.8 3.6 2.2 2.3 2.0 2.5 3.0 3.5 3.6 wool
effect 2.5 1.4 1.5 1.5 2.0 1.7 2.1 2.0 3.6 1.8 2.0 1.8 1.8 1.8 2.5 2.1 Hair growth
effect 2.6 1.5 1.7 1.4 1.9 1.7 1.9 1.9 3.7 1.8 1.8 1.7 2.0 2.0 2.3 2.0 Hair growth
effect 2.2 1.5 2.0 1.5 2.0 1.5 1.6 1.7 2.9 1.8 2.2 1.9 2.0 1.8 2.0 2.1

division 75 days past 90 days past end I All la hemp bar four Ah end I All la hemp bar four Ah hair loss
Prevention 3.7 2.5 2.6 2.7 2.5 2.8 3.5 3.6 4.0 2.7 2.6 3.1 3.0 2.9 3.8 3.7 wool
effect 3.8 1.9 1.9 2.5 2.0 2.2 3.0 2.9 3.9 2.8 2.8 3.0 3.2 2.7 3.5 3.6 Hair growth
effect 3.8 2.1 2.5 2.0 2.4 2.1 2.7 2.3 3.9 2.0 2.2 2.5 2.6 2.7 3.4 3.1 Hair growth
effect 3.1 1.7 2.3 2.0 2.0 1.9 2.3 2.2 3.2 2.1 2.2 1.9 1.9 2.3 2.9 2.7

Claims (3)

A nicotinoyl tripeptide represented by the following general formula (1), a biotinoyl tripeptide represented by the following general formula (2), a broiler chick extract, a Meadowweed extract, an Ephroobiite extract, And wintergreen extract as an active ingredient, and a composition for promoting hair loss and hair growth.
[Chemical Formula 1]

(2)

The method according to claim 1,
The broiler chick extract, chrysanthemum morifolium extract and wintergreen extract were extracted using 50 to 100% (v / v) ethanol as a solvent,
The Meadow's Sweet extract is obtained by extracting with a mixed solution of butylene glycol and water as a solvent,
Wherein the epilobium plaque kerri extract is extracted using a mixed solution of glycerin and water as a solvent, wherein the composition is for preventing hair loss and promoting hair growth. 3. The method of claim 2,
Wherein the nicotinoyl tripeptide is 0.001 to 5 wt%, the biotinol tripeptide is 0.001 to 5 wt%, the broiler chick extract is 0.1 to 10 wt%, the meadow extract is 0.1 to 10 wt% Wherein the extract of Angelica keiskei is 0.1 to 5% by weight, the extract of Angelica keiskei keelis is 0.1 to 5% by weight, the extract of kiwifruit is 1-10% by weight and the extract of wintergreen is 0.1 to 3% A cosmetic composition for promoting hair growth.

Images