KR102534945B1 - Cosmetic Compositions for Skin Moisture Comprising Callus Extract of Sageretia thea - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing containing a Sageretia thea callus extract as an active ingredient. Since the callus extract provided by the present invention has an excellent hyaluronic acid production effect, the callus extract can be used as a cosmetic composition for skin moisturizing.

Description

Cosmetic Compositions for Skin Moisture Comprising Callus Extract of Sageretia thea}

The present invention relates to a cosmetic composition for moisturizing the skin comprising a callus extract of Sageretia thea as an active ingredient.

The skin becomes sensitive due to changes in the environment, stress caused by internal factors, and irritation caused by external factors, and its ability to retain moisture may decrease. phenomena such as appearing. When the moisture of the skin decreases, the skin becomes dry, loses elasticity, and wrinkles are formed. In addition, the skin tone darkens and the skin barrier function deteriorates, making it more sensitive to external stimuli, which can increase various troubles. As such, the moisturizing state of the stratum corneum is directly related to the appearance of the skin (skin flexibility and roughness), and the skin moisture content of the stratum corneum affects the skin membrane homeostasis related to the condition and permeability of the skin. The moisture absorption of medicines can change, so skin moisturizing is an important factor. Therefore, there is an increasing demand for a cosmetic composition that maintains the moisturizing power of the skin so that the skin does not lose moisture and elasticity and maintains the effect.

On the other hand, homologous tree (Sageretia thea ) belongs to the genus Sageretia, and about 10 species are distributed in eastern and southern Asia and North America. It grows on the warm southern coast of Korea, on the southern islands, and at the foot of the seaside of Jeju Island. The flowering period is from September to November, and the fruiting period is from April to May of the following year. Fruits are mostly round in black purple. The Sangdong tree is also called the Jeonggeum tree, and the herbal name is Jakmaedeung (雀梅藤), and it is also called Daejeolja, Daegakja, Ssaemija, Sakyunggeun, and Mischaemok by other names. The leaves and twigs of the homologous tree have a strong detoxifying effect and are mainly effective in releasing scabies and poison ivy. In addition, it is a component that makes new skin sprout, and it is very effective for most skin diseases, bruises, scabies, wounds, and burns. The root is called Jaumeeunggeun (雀梅藤根), and is known to be effective for arthritis (Bae Gi-hwan, Korean medicinal plant). In addition, it has been used as a folk remedy for cold treatment, hepatitis, etc., and a novel antioxidant flavonoid substance among the ethyl ether soluble fractions of the leaves of Homo sapiens has been known.

As for the composition technology using S ageretia thea extract, in Korea, Korean Patent Registration No. 10-2171209 "Pharmaceutical composition and health functional food for preventing or treating thrombosis containing homologous extract as an active ingredient"", Republic of Korea Patent Publication No. 10-1831981 "Composition for preventing, improving or treating cancer containing an extract or a fraction thereof as an active ingredient", Korean Patent Publication No. 10-1743200 "Containing Homology extract or a fraction thereof as an active ingredient" A composition for skin whitening containing the fraction as an active ingredient” is disclosed.

Accordingly, the inventors of the present invention found that the callus extract of Sageretia thea can impart an excellent skin moisturizing effect and completed the present invention.

Republic of Korea Patent Registration No. 10-2171209 Republic of Korea Patent Registration No. 10-1831981 Republic of Korea Patent Registration No. 10-1743200

An object of the present invention is to provide a cosmetic composition for moisturizing the skin derived from natural products.

Another object of the present invention is to provide a cosmetic comprising a cosmetic composition for moisturizing the skin derived from natural products.

In order to achieve the above object, the present invention provides a cosmetic composition for moisturizing the skin comprising Sageretia thea callus extract as an active ingredient.

In the present invention, the extract is characterized in that the extract is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof.

In the present invention, the extract is characterized in that it comprises a fraction of the extract.

In the present invention, the fraction is characterized in that it is a solvent fraction selected from the group consisting of n-hexane, ethyl acetate, butanol and water of the extract.

In the present invention, the extract is characterized in that it comprises an enzyme-treated extract.

In the present invention, the enzyme-treated extract is characterized in that it is obtained by treating the extract with pectinex, a glycolytic enzyme.

In the present invention, the extract is characterized in that it comprises a fraction of the enzyme-treated extract.

In the present invention, the extract is characterized in that it contains 0.001 to 20% by weight based on the total weight of the composition.

In the present invention, the extract is characterized in that it has a hyaluronic acid producing effect.

In addition, the present invention provides a cosmetic comprising the composition.

Since the callus extract provided from the present invention has an excellent hyaluronic acid production effect, it can be used as a cosmetic composition for moisturizing the skin.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclatures used herein are those well known and commonly used in the art.

Throughout the present specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated.

According to one embodiment of the present invention, the present invention relates to a cosmetic composition for moisturizing the skin comprising Sageretia thea callus extract as an active ingredient.

The extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention pertains, and specifically, from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof. It may be extracted with a selected solvent.

Specifically, the content of the extraction solvent may be 2 to 100 times, more preferably 5 to 50 times, and most preferably 10 to 20 times the weight of Sageretia thea callus.

In addition, the extract may be extracted by heating or at room temperature under conditions in which the active ingredients of the extract are not destroyed or destruction is minimized. The extraction method is not particularly limited, and ultrasonic extraction, cold needle extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction, etc. may be used depending on the type of extract and extraction method.

Filtration is a process of removing floating solid particles from the extract, and particles may be filtered out using cotton, nylon, paper, etc., or ultrafiltration, cryofiltration, centrifugation, etc. may be used, but is not limited thereto.

The step of concentrating and then drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a step of pulverizing the finally dried extract may be added.

According to one embodiment of the present invention, the extract may be processed and used in various forms, such as a fermentation broth, a dried powder of the extract, and a concentrated filtrate of the extract.

According to one embodiment of the present invention, the extract may include a fraction of the extract.

The fraction may be a solvent fraction selected from the group consisting of n-hexane, ethyl acetate, butanol, and water of the extract.

According to one embodiment of the present invention, the extract may include an enzyme-treated extract.

The enzyme-treated extract may be obtained by treating the extract with pectinex, a glycolytic enzyme.

In addition, the extract may include a fraction of the enzyme-treated extract.

According to one embodiment of the present invention, the homologous tree ( Sageretia thea ) callus extract obtained from the above method may be included in an amount of 0.001 to 20% by weight, more preferably 0.1 to 1% by weight, based on the total weight of the composition. there is. If the extract is less than 0.001% by weight relative to the total weight of the composition, the function as a composition is poor because the hyaluronic acid production effect cannot be sufficiently obtained, and if it exceeds 20% by weight, sufficient efficacy can be exhibited even if it does not contain more than 20% by weight It is undesirable because it is uneconomical and it is difficult to use it as an external preparation because the stability and spreadability of the formulation are lowered.

Since the extract can give hyaluronic acid production effect, it has an effect of implementing fundamental skin moisturizing improvement.

As described above, since the extract has an excellent hyaluronic acid production effect, it can be used as a cosmetic composition for moisturizing the skin.

According to one embodiment of the present invention, when used as the cosmetic composition, external skin ointment, cream, softening lotion, astringent lotion, nutrient lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, spray , Gel, Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Eye Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, titanium oxide, etc. may be used as carrier components. can

In the case where the formulation of the present invention is a body gel, in addition to the above-mentioned active ingredients, carbomer, triethanolamine, menthol, ethanol, glycerin, disodium EDTA, methylparaben, fragrance, etc. this can be used Here, carbomer and triethanolamine act as a thickener, menthol acts as a fragrance component, ethanol functions to impart a refreshing feeling, glycerin acts as a humectant, and disodium EDTA acts as a metal component chelate. and methylparaben is added to function as a preservative. In addition, various flavors such as herbal flavors may be added to the flavor in addition to the menthol flavor.

In addition to the specific components described above, preparing a composition by applying known materials widely used in the art to achieve the functions such as thickener, cool feeling, fragrance component, and moisturizer described above is also within the scope of the present invention. can be included

When the formulation of the present invention is a powder or spray (spray), lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be added as a carrier component, especially in the case of a spray, additionally chlorofluorohydro It may contain a propellant such as carbon, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is added as a carrier component. Examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene glycol. , 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, and sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

According to one embodiment of the present invention, the cosmetic composition may be prepared as a composition such as body gel, body lotion, body cream, body oil, etc. for promoting lipolysis by adding conventional additives, and may also be prepared as an aerosol type. can In this case, the cosmetic composition is preferably used as a method of transdermal administration by applying or spraying directly on the skin.

According to one embodiment of the present invention, the amount of the cosmetic composition used can be appropriately adjusted according to individual differences such as age, degree of skin fat condition, formulation, and shape, and can be usefully used as a composition for skin moisturizing effect. .

The cosmetic composition obtained by the above method can provide excellent cosmetics by exhibiting hyaluronic acid production efficiency.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-explanatory.

<Sample> Homologous tree callus induction and proliferation

The collected homologous tree seeds were sterilized and sterilized, and aseptic in vitro plants were induced using MS medium (Murashige and Skoog) supplemented with 30 g/L. Induced plants were cut and growth regulators (0.5 mg/L 2,4D, 0.5 mg/L NAA, 0.1 mg/L kinetin, 100 mg/L ascorbic acid, 30 g/L sucrose, 2.4 g/L gelrite) was added and cultured for 4 or 8 weeks at 25±1°C in the dark. The medium was adjusted to pH 5.8 using 1N potassium hydroxide and cultured in a 3 L balloon bioreactor with an air injection volume of 0.1 vvm. Homologous tree callus was washed and dried through freeze-drying and hot-air drying, and then used in the experiment.

<Examples 1 to 4> Manufacture of homologous tree callus extract

1 L of 75% (v/v) ethanol aqueous solution was added to 50 g of Homo sapiens callus, followed by extraction by heating at 60-70 ° C. for 3 hours, followed by filtration. The extraction was repeated twice, and the obtained extract was concentrated under reduced pressure to pulverize the extract to obtain a 75% (v/v) ethanol extract (Example 1).

Distilled water was added to the ethanol extract of Example 1 to dissolve it, and then fractionated and concentrated with ethyl acetate to obtain an ethyl acetate fraction (Example 2).

A 10% (v/v) ethanol aqueous solution was added to the ethanol extract of Example 1 to dissolve it. Pectinex (Novozymes, Denmark), a glycolytic enzyme, was added thereto, reacted at 50-60 ° C for 30 hours, filtered, and concentrated under reduced pressure at 40 ° C to obtain an enzyme-treated extract (Example 3).

Distilled water was added to the enzyme-treated extract of Example 3 to dissolve it, and then fractionated and concentrated with ethyl acetate to obtain an ethyl acetate fraction (Example 4).

<Comparative Example 1> Manufacture of homologous tree leaf extract

1 L of 75% (v/v) ethanol aqueous solution was added to 50 g of homologous tree leaves (collected), extracted by heating at 60 to 70 ° C. for 3 hours, and then filtered. The extraction was repeated twice, and the obtained extract was concentrated under reduced pressure to pulverize the extract to obtain a 75% (v/v) ethanol extract (Comparative Example 1).

<Experimental Example 1> Skin moisturizing test: hyaluronic acid production effect

The skin moisturizing effect was confirmed using the extracts of Examples 1 to 4 and Comparative Example 1.

First, human-derived keratinocytes were purchased from ATCC (American Type Culture Collection), and DMEM medium (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) containing 10% FBS (fetal bovine serum, Gibco BRL, USA) was used. were incubated at 37°C and 5% CO ₂ . The cultured cells were dispensed in a 24-well plate at 1X10 ⁵ cells/mL per well, and cultured for 24 hours. After culturing, samples of the extracts of Examples 1 to 4 and Comparative Example 1 were replaced with serum-free DMEM medium diluted by concentration and cultured for 24 hours. The amount of hyaluronic acid synthesis was measured using a hyaluronic acid measurement kit (Hyaluronan ELISA kit, Echelon Biosciences, USA). The absorbance of the cell culture medium not treated with the sample was set as 100% of the control group, and the experiment was performed three times each and expressed as the average value ± standard deviation.

division Hyaluronic acid production effect control group 100 Example 1 146.62 ± 2.01 Example 2 129.56 ± 1.05 Example 3 153.71 ± 1.54 Example 4 252.19 ± 9.90 Comparative Example 1 127.43 ± 1.80 Retinoic acid (positive control) 126.87 ± 2.59

As a result, as can be seen in Table 1, Examples 1 to 4 exhibited a hyaluronic acid production effect compared to the positive control group and Comparative Example 1, and in particular, it was confirmed that the hyaluronic acid production effect was significantly superior in Example 4.

<Experimental Example 2> Skin moisturizing effect evaluation

For the skin moisturizing effect of the extracts of Examples 1 to 4 and Comparative Example 1, Example 4 with excellent efficacy was performed based on the evaluation results of the extracts of Examples 1 to 4 and Comparative Example 1. As shown in Table 2 below, the composition containing Example 4 was a cream-type composition prepared by a conventional manufacturing method.

Unit (weight %) ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 formulation example One Cetearyl Alcohol 1.0 1.0 1.0 2 Glyceryl Stearate/PEG-100 Stearate 1.0 1.0 1.0 3 polysorbate 60 1.0 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 0.3 5 cetyloctanoate 6.0 6.0 6.0 6 squalane 8.0 8.0 8.0 7 Africord Kernel Oil 4.0 4.0 4.0 8 dimethicone 2.0 2.0 2.0 9 glycerin 5.0 5.0 5.0 10 Magnesium aluminum silicate 0.4 0.4 0.4 11 xanthan gum 0.03 0.03 0.03 12 Preservatives and Flavors a very small amount a very small amount a very small amount 13 Purified water (to 100) balance balance balance 14 Example 4 - - 5.0 15 Comparative Example 1 - 5.0 -

As subjects, 20 women aged 20 years or older were given a cream (Formulation Example) containing the composition containing Example 4 on the left side of the face, and a cream not containing the composition containing Example 4 (Comparative Formulation Example 1) on the right side of the face. and 2) were continuously used twice a day for 12 weeks.

In the test, a survey was conducted on the subjective efficacy of the moisturizing effect of the skin. In the evaluation of skin irritation, phenomena such as itchiness, stinging, and erythema of the skin were evaluated. The evaluation was performed according to the five-point criteria of very good (5 points), excellent (4 points), average (3 points), bad (2 points), and very bad (1 point), and the results are shown in Table 3 below. Same as

number moisturizing effect formulation example Comparative Formulation Example 1 Comparative Formulation Example 2 One 5 4 5 2 4 4 4 3 5 3 3 4 5 5 4 5 4 3 4 6 4 4 5 7 5 4 4 8 4 4 5 9 4 3 4 10 5 4 4 11 5 4 5 12 4 3 3 13 4 4 5 14 5 5 4 15 4 4 5 16 4 4 4 17 5 4 5 18 5 4 3 19 5 3 5 20 4 4 3 average 4.50 3.85 4.20

As a result, as can be seen in Table 3, the skin moisturizing effect evaluation score of the formulation example was 4.50 points, showing excellent effects compared to Comparative Formulation Examples 1 and 2.

Having described specific parts of the present invention in detail above, it will be clear to those skilled in the art that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the invention will be defined by the appended claims and their equivalents.

Claims (10)

A cosmetic composition for moisturizing the skin comprising an ethyl acetate fraction of a callus extract of Sageretia thea enzyme-treated with Pectinex as an active ingredient.


delete delete delete delete delete delete delete The method of claim 1, wherein the ethyl acetate fraction of the homologous tree ( Sageretia thea ) callus extract is enzyme-treated with pectinex, characterized in that it has a hyaluronic acid production effect. A cosmetic composition for moisturizing the skin, comprising as a component.
A cosmetic comprising the composition of any one of claims 1 and 9.