KR100771829B1 - A composition comprising plant extracts for protecting skin from stress due to harmful environment - Google Patents

Abstract

A cosmetic composition comprising a plant mixture extract is provided to alleviate skin damage caused by stress of the outside environment and show skin protecting effect by inhibiting over-reaction and erythema and maintaining skin moisture, thereby being useful as a skin protecting cosmetic composition. A cosmetic composition having skin protecting effect from active oxygen, skin dryness and allergy comprises a plant mixture extract consisting of 10-15% of green tea, 5-10% of Pinus sylvestris, 5-10% of Glycyrrhiza uralensis, 5-10% of Paeonia suffruticosa Andrews, 8-12% of Scutellaria baicalensis George, 5-10% of Sophora flavescens, 1-5% of Glycine max, 1-5% of aloe, 1-5% of Mori Cotex Radicis, 5-10% of persimmon leaves, 1-5% of kiwi, 5-10% of Polygoni Multiflori Radix, 5-10% of camellia and 10-15% of Panax ginseng, wherein the extract is prepared by extracting the mixture using water, ethanol, methanol, propanol, butanol, acetone, ethylacetate, hexane, benzene, chloroform, glycerine, butylene glycol or propylene glycol at a temperature of 10-37 deg.C for 1 to 15 days and the content of the dried extract is 0.01-10.0 weight/volume %.

Description

A composition comprising plant extracts for protecting skin from stress due to harmful environment

The present invention contains a mixed extract obtained from green tea, pine needles, licorice, bark skin, golden, ginseng, soybean, aloe, lettuce, persimmon leaf, kiwi, sewage, camellia, and ginseng, the effect of protecting the skin from stresses caused by harmful environment It relates to a cosmetic composition having a.

The skin is the largest organ in the body and is always in direct contact with the external environment and acts as a protective shield to protect the living body from various irritation or dry environments. It is an organ in which new cells are actively produced and destroyed compared to other organs. It also plays a very important role in protecting the body from physical abrasions, preventing the loss of moisture inside the body, protecting it from harmful ultraviolet rays from the sun, and regulating the body's body temperature.

Baby skin, like adults, protects the body from germs and releases sweat and heat to regulate body temperature. In addition, the function of the internal organs, such as lungs and kidneys to help remove the waste in the body, but it is different from adults. In addition to the skin, the main appendages, such as the sweat glands, are still immature, so they lack the ability to control their body temperature by sweating and lowering their temperature. In addition, the baby's skin easily loses moisture, dries out and becomes less susceptible to damage. In this way, the skin of the baby is the same as the adult but the appearance is insufficient in function, and after the age of 3 is the thickness similar to the adult skin, so the moisture of the baby's skin is a basic requirement to maintain healthy skin, it is important.

In addition, the baby's skin is soft and thin, fragile and sensitive to external stimuli and bacterial infections, and the penetration rate is much faster than adults, so sweat and bacteria remaining on the skin penetrate into the skin again, causing the skin to crush and Skin problems may occur. Therefore, baby skin tends to cause skin troubles even with small external stimuli, so it is also important to select and use raw materials that protect skin and strengthen skin against external stimuli. <BAYER KOREA (Bayer Korea), BAYER HEALTHCARE >.

The stress of baby skin caused by external stimulation can be divided into physical factors and external environmental factors. Physical factors include, first of all, bad skin care in the baby's bath. Damage to the stratum corneum due to too frequent bathing, long bathing times, or improper care such as using towels or cotton towels, results in lack of natural moisturising factors, or in extremely poor weather conditions. You lose. The baby's skin is dry and taut, and the nourishing cream is hardly absorbed. As the nutrition is not supplied to the skin, the protection of the skin becomes lower and the transepidermal water loss (TEWL) increases and the barrier function of the skin becomes more damaged.

The second is the use of products that are not suitable for baby's skin, products with irritating ingredients, and products that have been improperly stored and altered. Cosmetics are products used for skin protection, beautification and cleanliness, but if you look closely at the cosmetic composition, ingredients different from the original skin protection purpose are indispensable for the formation of the product. For example, there are solubilizers, emulsifiers, preservatives, fragrances, sunscreens and other additives used to impart efficacy and effects, which are commonly the main causes of skin irritation, rashes, edema or allergies. Known as In addition, when using cosmetics, environmental conditions such as temperature, humidity and the wrong use method, the user's constitution and physical condition are often caused by inflammation and allergies.

The stress caused by external environmental factors is the first stress caused by UV rays such as the generation of free radicals, photoallergy, and weakened skin immune system. UV damage to the skin is known a lot. UV B depends not only on geographic factors such as latitude and altitude, but also on the level and time of air pollution, which usually causes acute damage such as DNA damage and erythema. In addition, cells involved in antigen expression in the epidermis, such as Langerhans cells, are damaged, resulting in partial immunity. UV A penetrates deep into the connective tissue in the dermis, causing changes, causing irreversible damage and free radicals over long periods of time, making it an important stimulant for phototoxicity and photoallergy. The second is the stress caused by dry environment, which is the cause of deterioration of moisturizing ability due to the dry environment according to season and living conditions, resulting in deterioration and allergic diseases such as skin immune system deterioration and atopy. The third reason for the stress caused by external environmental factors is yellow dust. Recently, it has been reported that yellow sand contains sulphite gas, quartz (silicon), cadmium, lead, aluminum, copper, etc., as well as dioxin. Thus, thorough health and skin care are required. Fine dust from yellow dust irritates and dries the skin, causing itching or itching. Especially in patients with atopic dermatitis, the symptoms worsen, such as severe itching and red spots. Moreover, baby skin is fragile and incomplete, causing an allergic reaction, leading to a high incidence of contact dermatitis. In addition, dry and strong yellow dust winds take away moisture from the skin and cause dry skin, resulting in white keratin. In addition, there are various soot as a cause of air pollution, various skin diseases caused by bird house syndrome as a cause of indoor pollution, the occurrence and exacerbation of atopy, allergens caused by pollen and the like. Stress of baby skin due to the above mentioned physical factors can be avoided with parents 'attention, but stress caused by external factors is not enough with parents' attention.

Stress caused by external environmental factors can cause contamination, dryness, problems with the skin's immune system, and ultimately cause atopy, allergies and deterioration. need. Therefore, new attempts are being made to keep the baby's skin healthy and strong from stress caused by such external factors.

Therefore, the inventors of the present invention is a mixed extract obtained from green tea, pine needles, licorice, bark skin, golden, ginseng, soybeans, aloe, lettuce, persimmon leaf, kiwi, sewage, camellia and ginseng have excellent safety on the skin and alleviate skin damage caused by stress and The present invention was found to have a skin protective effect.

The present invention includes a blend of extracts from green tea, pine needles, licorice, bark skin, golden, red ginseng, soybean, aloe, lettuce, persimmon leaf, kiwi, sewage, camellia and ginseng, to alleviate skin damage due to stress caused by external environment and It is an object to provide a composition that gives a skin protective effect.

The present invention also aims to provide a cosmetic composition comprising the above mixed extract.

Therefore, in one embodiment of the present invention from green tea, pine needles, licorice, bark skin, golden, ginseng, soybean, aloe, lettuce, persimmon leaf, kiwi, sewage, camellia and ginseng having the effect of protecting the skin from stresses derived from harmful environment It provides a composition comprising the obtained mixed extract.

In the raw material of the mixed extract contained in the composition of the present invention,

Thea sinensis Linne ) is one of the dried young leaves of the tea tree, which is a evergreen blue evergreen tree, the leaves are thick, long oval, and white flowers bloom in autumn. Depending on the processing method, it is divided into green tea, black tea, and flower tea. The green tea is dried after steaming or inactivating oxidase by hot wind without fermenting young leaves. Dark teas have long been used to prevent sunburn.

Pinus densiflora ) is a spring dried young shoots of the pine family belonging to the Pinaceae family. It is a evergreen tree, with bark reddish brown and two leaves. Young shoots are dried in spring. Spring young shoots have also been used to treat scurvy.

Licorice ( Glycyrrhiza glabra L. ) is a perennial herb that is 30-80 cm high, legume (Leguminosae). Branches of red flowers gather together like Isaac. It is planted as a herb in various places around the central region of Korea, and has anti-inflammatory action. This action is known to be due to its main component, glycyrrhizin. Glycirizine is about 40 to 50 times sweeter than sweet. It is not toxic and has a detoxifying effect against various poisonings, eg bacterial poisons. Licorice flavonoids have excellent anti-inflammatory properties and are good ingredients for preventing and relieving inflammation. One of the flavonoids, liqueurside, has three times as strong as papaverine and has anti-inflammatory action. In addition, it has antihistamine and bactericidal action, and is used for the purpose of alleviating and harmonizing the action of other raw materials.

Paeonia suffruticosa ) refers to the root shell of peonies. In autumn, the roots of the peonies are carved and washed in water, then the neck is removed and dried in the sun. It is not poisonous and slightly cold, so it is also used to treat menstrual irregularities, hyperemia, bruises and skin rashes. It is a deciduous broad-leaved shrub. The leaves are divided into two upper right bilobes. The small leaves are ovate or lanceolate, and are divided into three branches or coarse teeth. Flowers are large and are endemic to the end of branches. Flowers bloom in May, fruits are bone stones, nucleus, dense short hairs, ripen in autumn. Pheonol components in the bark skin have anti-inflammatory and sedative effects and hemostatic effects. In particular, the anti-inflammatory action of pheonol inhibits swelling of rat hind legs by acetic acid.

Golden ( Scutellaria baicalensis ) is a perennial herb that is about 1m high. Willow leaf-shaped leaves are facing each other, and light branched flowers gather in summer. The roots of this plant are plucked in spring or autumn, discarding the husks and rotten genus, washed in water and dried in the sun. Its root is yellow and it is called golden. It is a raw material with multifunction such as active oxygen scavenging, uv absorption, anti-allergic, anti-inflammatory and antibacterial action.

Ginseng ( Sophora angustifolia Sieb. ) Is a legume, also known as the cane of thieves, the ginseng, and the sperm of snakes. It grows in sunny meadows. It is green with 80-100cm height, but it is black when young. Stems are straight, leaves are alternate, oddly littered leaf, 15-40 small leaves, long oval or long egg-shaped, 2-4cm long, 7-15mm butterfly. The petiole is long and the edge is flat. A flower stem of about 20cm comes out at the end of the branch in June-August and pale yellow flowers in the shape of butterflies bloom in total. The length of the flower is 15-18mm. Calyxes look like barrels, have hairs on the outside, and have five end tips that are shallowly divided. Fruits are in the form of beads in the form of beads. It has a short stem and is 7-8cm long and ripens in September-October. In oriental medicine, dried root is called ginseng, and it is tasteless and has the effect of ginseng, which is prescribed for indigestion, neuralgia, hepatitis, jaundice, and hemorrhoids. It has strong anti-allergic activity and also has anti-inflammatory action. It is used for scabies, eczema, dry ringworm, etc. It is also used to treat malignancies. In addition, it has fungal and paralytic action.

Beans ( Glycine Max L. ) is an annual herb, also known as soybean (大豆). Beans are widely cultivated as edible crops, with stems of 60 to 100 cm in height, with a straight, vine-like variety. Roots have many roots. Soybeans contain the most protein. Eighty to ninety percent of soy protein consists of essential amino acids: arginine, methionine, threonine, leucine, isoleucine, and valine.

Aloe, also called presbytery or tree presbytery, refers to the whole plant of the genus Aloe or a species. It is native to Africa, has about 300 species worldwide, and is a houseplant that grows in greenhouses or grows at home for use as a medicine. Aloe leaves run on the roots and stems, alternate, semi-cylindrical, rounded on the back of the leaves, slightly in front, with sharp serrated spines on the edges of the leaves, wide at the base, surrounding the stems, and spreading in rosette shape. Flowers bloom orange in summer and run downwards, forming a gunshot inflorescence. It contains aloetin, which is bactericidal and fungicidal to bacteria and fungi, and contains aloeursin, which is effective against ulcers, and aloemitin, which has anticancer effects. Other ingredients include steroids, amino acids, saponins, antibiotics, wound healing hormones, and minerals.

Morus ( Mori) cortex radicis ) is a mulberry root bark that is used in spring or autumn to rip roots and scrape off the bark or dry it in the sun. It is 1-4mm thick and its outer surface is white or pale yellow white. It is relatively flat and has yellow or yellowish brown scale pattern. The inner surface has longitudinal wrinkles that turn yellowish yellow or grayish yellow.

Persimmon Leaf ( Diospyros kaki L. ) is the leaf part of persimmon tree, and the persimmon tree is the leafing tree of Ebenaceae. Its height is 12-15m. The leaves are oval-shaped and stick together. Female and male flowers bloom separately on one tree, and they grow or plant in the mountains of the central and southern parts. Persimmon leaves contain a large amount of vitamin C and vitamin P to increase immunity and resistance, and a lot of alkaline components to increase the blood to improve the metabolic activity of the cells to help proliferation.

Kiwi ( actinidia) chinensis YANGGAO ) is native to the forests of 100-1600m above sea level in Japan, China and Korea. It is also called daerae, and the fruit grown in fruit farms is called daerae or yangdae. Its length is 7m, the trunk of the stem is brown, staircase, fine hairs on young branches, and the trunk is clear. Fruits are egg-shaped circular and ripe in yellow green in October. Kiwi has Vit. C, Vit. A, calcium, Vit. B6, copper, Vit. E, fiber, potassium, etc. are contained. Among them, Vit. The high content of C increases resistance and has a great effect on anti-aging and relieving stress of blood vessels.

Sewage (Polygonum multiflorum Thunberg) is native to China and is grown as a medicinal plant. Root stems extend into the ground, forming thick tubers like sweet potatoes. The main stem is long, branched and hairless. Leaves are alternate, egg-shaped heart-shaped, pointed end, leaf edge is flat, and chin leaf is short, cylindrical. At the bottom of petiole is short petiole. Reddish brown tubers are called sewage in Chinese medicine and used as tonic, tonic and laxative.

Camellia ( Camellia) Japonica L.) is a flower of the evergreen arborescent camellia, which belongs to the tea tree family, and is also called 山 茶花. It is a flower of the evergreen cultivated tree in the mountains and villages near the coast of Ulleungdo and Daecheongdo, south of Korea. It is red in color. Calyxes are 5, different in length, oval-shaped, 1 ~ 2cm long. The petals are 5 sheets at the bottom, and they are attached to the surgery again. The stamens are numerous. Hwasa is white and attached to each other. The anther is yellow.

Panax ginseng schinseng Nees ) is a perennial plant that grows in deep forests all over our country. Its height is 60cm, roots are rods, roots are short, stand straight or at an angle, and one stem comes out at the end. At the end of stem, 3-4 leaves are limbal, petioles are long, and the leaves are palm-shaped double leaves. Small leaves are ovate or obovate, pointed at the end, and there are fine serrated edges. Flowers are male and female, light green, and one umbel is hanged at the end of the long axis from the limbal leaf at the end of the stem. Fruits flatten and ripen red in a round shape. Ginseng saponin, the main ingredient of ginseng, is effective for treating or preventing skin wounds and troubles by reviving skin cells or mucosal cells by supplying nutrients necessary for skin cells or mucosal cells.

The plant mixture extract included in the composition of the present invention may extract the raw plant using water, an organic solvent or a mixed solvent thereof. All of these components may be mixed and extracted at the same time, or each component may be extracted and mixed under optimum conditions.

As an extraction solvent for preparing the mixed extract of the present invention, a group consisting preferably of water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, and propylene glycol One or more solvents selected from can be used. Preferred amounts of extraction solvent are 5 to 30 times the volume amount of the plant raw material.

The mixed extract included in the composition of the present invention can be obtained by various extraction methods. Specifically, extraction may be performed at room temperature or by heating using these solvents or a mixture of solvents, or may be extracted using a single method or a mixture of various known methods for natural products. Preferably it is extracted by dipping in a solvent at 10-37 degreeC for 1 to 15 days, or it extracts by heating at 50-100 degreeC for 3 to 20 hours by adding a solvent.

The composition ratio of the mixed extract included in the composition of the present invention is not particularly limited, but as demonstrated by the following examples, green tea 10-15%, pine needles 5-10%, licorice 5-10%, bark 5-10 %, Golden 8-12%, red ginseng 5-10%, soybean 1-5%, aloe 1-5%, lettuce skin 1-5%, persimmon leaves 5-10%, kiwi 1-5%, sewage 5-10%, When it is used in the range of 5 to 10%, 10 to 15% ginseng flower, it can be seen that particularly excellent skin protection effect.

In one preferred embodiment of the present invention provides a cosmetic composition comprising the above-mentioned mixed extract. The total weight of the mixed extract included in the cosmetic composition is, in its dry weight, in the range of about 0.01 to 20%, preferably 0.5 to 10% of the total composition weight.

The plant used in the composition according to the present invention is a material that has been used for a long time and has less toxicity and side effects of each sample itself. Therefore, the composition containing the natural plant extract according to the present invention can be used as a cosmetic composition without concern for toxicity and side effects.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Cosmetic compositions that protect the skin from the stress caused by the harmful environment of the present invention may be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence And hair cosmetics. Preferably baby lotion (skin lotion), lotion, cream, sunscreen lotion, sunscreen cream, baby bath cleaner, shampoo, soap.

Specific examples of the cosmetic of the present invention include a flexible lotion, nourishing lotion, nourishing cream, essence, cleansing cream, cleansing foam, cleansing cream, cleansing lotion, massage cream, cold cream, moisturizing cream, pack, aftershave cream, tanning cream, suntan Dragon oil, body shampoo, hair shampoo, hair rinse, hand cream and foundation, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, plant fibers, waxes, paraffins, starches, tragacanths, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide are used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

The following Examples and Test Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Spare Experimental Example  One.

In order to know the difference in efficacy according to the content ratio before extracting with various solvents to obtain the best effect of alleviating and protecting the skin damage caused by stress derived from the external environment Using the method of 1 to determine the efficacy after extraction. For the preliminary efficacy test, the anti-inflammatory effect (inhibition of iNOS activity) was selected in Experimental Example 5, and the moisturizing effect was compared to after the experiment using the experimental method of Experimental Example 7. The results are shown in Table 2.

Content ratio by raw material green tea Pine cone licorice Neck skin Gold Gosam bean Aloe White skin Persimmon leaf Kiwi Shouao Camellia Ginseng Preliminary Example 1 One One One One One 0 0 0 0 0 0 0 0 0 Preliminary Example 2 One One One One One One One One 0 0 0 0 0 0 Preliminary Example 3 One One 0 0 0 0 One One 0 0 One 0 One 0 Preliminary Example 4 One 0 0 0 0 0 One 0 One One One One One One Preliminary Example 5 One One One One One One One One One One One One One One Preliminary Example 6 One One One One One One One One 2 2 2 2 2 2 Preliminary Example 7 One One One One 2 2 One 2 2 2 3 3 3 3 Preliminary Example 8 One One One 3 3 3 One One 2 3 3 2 4 4 Preliminary Example 9 2 One One One One 2 2 2 2 2 One One One 2 Preliminary Example 10 2 2 2 2 2 2 2 2 One One One One One One Preliminary Example 11 3 3 3 3 2 2 2 2 One One One One One One Preliminary Example 12 4 2 2 2 3 2 One One One 2 One 2 2 4 Preliminary Example 13 4 3 3 2 2 2 2 2 One One One One One One Preliminary Example 14 4 4 4 3 3 2 2 2 One One One One One One

Anti-inflammatory and moisturizing effect Preliminary Example Application concentration (ug / ml) iNOS activity inhibition (%) Moisturizing Growth Rate Preliminary Example 1 200 10.2 0.93 Preliminary Example 2 200 12.7 0.98 Preliminary Example 3 200 12.1 0.98 Preliminary Example 4 200 14.8 1.01 Preliminary Example 5 200 16.7 1.01 Preliminary Example 6 200 19.9 1.04 Preliminary Example 7 200 20.5 1.02 Preliminary Example 8 200 25.8 1.08 Preliminary Example 9 200 28.1 1.14 Preliminary Example 10 200 24.9 1.08 Preliminary Example 11 200 29.7 1.07 Preliminary Example 12 200 45.8 1.21 Preliminary Example 13 200 32.6 1.05 Preliminary Example 14 200 37.2 1.13

Example  1. Mixed Extract Manufacturer

95% of mixed raw materials consisting of green tea 40g, pine sprout 20g, licorice 20g, bark peel 20g, golden 30g, red ginseng 20g, soybean 10g, aloe 10g, lettuce white pepper 10g, persimmon leaf 20g, kiwi 10g, sewage 20g, camellia 20g and 40g ginseng The mixture was boiled in 10 kg of distilled water and extracted by boiling in an extractor equipped with a cooling condenser for 5 hours, filtered through a 400 mesh filter cloth, cooled to room temperature, left to mature at 5-15 ° C. for 7 days, and filtered through a filter paper of Whatman 2. The extract was filtered with a 0.45 μm filter and concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 10.98 g (dry weight).

Example  2 to 7

Using the solvent of Table 3 below and extracting in the same manner as in Example 1 and the results are shown in Table 3.

Example Extraction solvent Solid content (%) Yield (g) Example 2 Ethyl alcohol 26.2 473 Example 3 30% hydrous ethyl alcohol 29.4 498 Example 4 Butylene glycol 20.6 495 Example 5 30% water butylene glycol 23.5 479 Example 6 Propylene glycol 20.12 472 Example 7 30% water propylene glycol 22.1 474

Experimental Example  One. Superoxide From radicals  Cytoprotective effect experiment

Experiment method

Human fibroblasts were cultured in well plates at a concentration of 2 × 10 ⁵ cells / well, fed 0.2 ml of IMDM medium containing 10% FBS, and then incubated in a CO ₂ incubator for 24 hours. This was treated with a mixed extract dissolved in DMSO and incubated for 30 minutes. 10 μl of 1.5 mM xanthine and 10 μl of 0.25 U xanthine oxidase were added to the cultured cells to generate superoxide radicals, and then cultured in a CO ₂ incubator for 24 hours.

Then, MTT assay was performed to determine the protective capacity of the cells. 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed, and 0.5 ml of DMSO was added to formmazan. The absorbance was measured at 570 nm using an ELISA reader. As a control, vitamin C (Vitamin C, Sigma) having good radical scavenging ability was used.

 Cytoprotective Effect on Plant Mixture Extracts Example Final concentration (µg / ml) of the sample contained in the reaction solution Cell protective effect (%) Example 1 100 30 Example 2 100 37.8 Example 3 100 51.3 Example 4 100 53.4 Example 5 100 69.7 Example 6 100 49.6 Example 7 100 54.8 1) Control group (vitamin C) 50 60.2

Experimental Example  2. Experimental effect on protection of cell from ultraviolet rays

Experiment method

Human fibroblasts were cultured in well plates at a concentration of 2 × 10 ⁵ cells / well, 0.2 ml of IMDM medium containing 10% FBS, and then cultured in a CO ₂ incubator for 24 hours. After treatment with the plant mixture extract dissolved in DMSO and irradiated with UV light amount of 2 MED for 5 minutes and incubated for 24 hours.

Then, MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed, and 0.5 ml of DMSO was added to formmazan. The absorbance was measured at 570 nm using an ELISA reader. Antioxidant EGCG (Epigallocatechin Gallate, Sigma) was used as a control.

Cytoprotective Effect on Plant Mixture Extracts Example Final concentration (µg / ml) of the sample contained in the reaction solution Cell protective effect (%) Example 1 100 41 Example 2 100 35 Example 3 100 39 Example 4 100 43 Example 5 100 56.4 Example 6 100 31 Example 7 100 45 Control (EGCG) 25 52.9

Experimental Example  3. Photosensitization  Cell membrane protection from substances

Human red blood cells are optimal for use as a subject of study of living cell aging with free radicals by themselves. The erythrocyte membrane has been studied extensively structurally, and because of the advantages of easily obtaining a sample, it is a model of a biological membrane, a means of evaluating the effect on cell membranes such as natural products, pharmaceuticals, and cosmetic raw materials, or by active oxygen on the cell membrane. It is used as a target for understanding lipid peroxidation, protein damage and hemolysis mechanism.

Experiment method

The erythrocytes used in the experiment were collected from Albino rabbits of New Zealand as white blood cells were removed. The erythrocyte suspension had an OD of 0.6 at 700 nm and an erythrocyte count of 1.5 × 10 ⁷ cells / ml. 50 μl of the mixed plant extract was added to the red blood cell suspension, pre-incubated in the dark for 30 minutes, and then light-sensitized (rose-bengal) was added and irradiated. After 15 minutes of light irradiation, the degree of destruction of erythrocytes by post-incubation was determined from light transmittance (% transmittance) at 700 nm at 15 minute intervals. The effect of the mixed plant extract on photohemolysis was represented by τ ₅₀ indicating post-incubation time and degree of hemolysis. The τ ₅₀ value is the time taken for red blood cells to 50% hemolysis (minutes). Antioxidant EGCG (Epigallocatechin Gallate, Sigma) was used as a control. The results are shown in Table 6.

Cell membrane protective effect Example Final concentration (µg / ml) of the sample contained in the reaction solution Cell membrane protective action ( τ ₅₀ : min) Example 1 50 156 Example 2 50 165 Example 3 50 183 Example 4 50 192 Example 5 50 215 Example 6 50 154 Example 7 50 170 Control (EGCG) 50 210

Experimental Example  4. Experiment for promoting cell metabolism against plant extracts

Experiment method

This experiment compares the cell proliferation ability of FBS (fetal bovine serum) containing nutrients and various hormones needed for cell growth with that of plant mixture extract. This is an experiment to know the cell proliferation ability.

Human fibroblasts were put into a 24-well plate at a concentration of 5x10 ⁴ cells per well using a hemocytometer and incubated for 24 hours. After 24 hours of incubation, one side of the plant mixed extract was added to the IMDM medium, and 10% FBS (fetal bovine serum) was added to the IMDM medium for the control group. After incubation for 4 days, MTT assay is performed to determine the proliferation of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours, and then the medium was removed, and 0.5 ml of DMSO was added to formmazan. The absorbance was measured at 570 nm using an ELISA reader.

Promoting Cell Metabolism Activity of Plant Mixture Extract Example Final concentration of the sample contained in the reaction solution ㎍ (㎍ / ml) Promote cellular metabolic activity (proliferation) effect (%) Control (FBS added) Addition of plant mix extract (no FBS) Example 1 100 109 105 Example 2 100 111 106 Example 3 100 103 110 Example 4 100 105 109 Example 5 100 110 119 Example 6 100 109 107 Example 7 100 110 104

Experimental Example  5. iNOS  Activity inhibition experiment

Raw264.7 cells, which are macrophages of mice, were used to measure the activity of iNOS (inducible Nitric Oxide Synthase). Suspend 8 x 10 ⁵ cells in 10% FBS-DMEM (Fetal Bovine Serum, Fetal Bovine Serum, Dulbecco's Modified Eagle Medium, Gibco) medium containing no phenol-red to a 24-well culture plate. Inoculated and attached. After one day, the sample was incubated for 18 hours by concentration, followed by incubation for 24 hours by treatment with 1 μg / ml lipopolysaccharide (LPS, lipopolysaccharide, Sigma). Thereafter, the supernatant was collected, 100 µl each was added to a 96-well plate, and the same amount of Gries' reagent (Griess reagent, Sigma Co., Ltd.) was added thereto, and the mixture was gently shaken at room temperature for 10 minutes to measure absorbance at 570 nm. A calibration curve was prepared using sodium nitrite as a standard, and the amount of nitric oxide produced in each culture was determined. The% Yield of each sample was determined by making the amount of nitric oxide produced in the lipopolysaccharide treated group 100%. Alpha bisabolol (α-bisaborol, Sigma) used as a control is a substance that is widely used as an anti-inflammatory and anti-irritant.

Inhibitory Effects of Plant Mixture Extracts on iNOS Activity Sample (treatment concentration 100μg / ml) % Yield LPS + Example 1 49.5 LPS + Example 2 52.2 LPS + Example 3 55.9 LPS + Example 4 68.3 LPS + Example 5 73.2 LPS + Example 6 60.7 LPS + Example 7 61.9 Control group (α-bisaborol) 65.2

Experimental Example  6. Anti-allergy  Experiment (β- hexosaminidase  Emission measurement)

Beta-hexosaminidase Enzymes contained in mast cells are released with histamine when the mast cells are activated by an immune response. Therefore, it can be used as a degranulation indicator for mast cells. Planta Med. 64, 577-578 (1998)>

RBL-2H3 cells are incubated with EMEM containing 10% FBS and glutamine. Dispense ⁵ x 10 ⁵ cells per well into a 24 well plate. At this time, use medium containing 0.5μg / ml mouse monoclonal IgE in EMEM, and incubate in 37 ℃, 5% CO ₂ incubator for 24 hours. Rinse the cells with 500 μl of siraganian buffer I per well, add 160 μl of siraganian buffer II and incubate in 37 ° C., 5% CO ₂ incubator for 10 minutes. . After treatment with the plant mixture extract, incubated for 20 minutes in a 37%, 5% CO ₂ incubator and treated with antigen (1 μg / ml, DNP-BSA) for 10 minutes. The reaction is stopped in a ice bath for 10 minutes to stop the reaction and centrifuge. 20 μl of substrate (1mM ρ-nitrophenyl-N-acetyl-β-D-glucosaminide) was added to the supernatant, followed by incubation for 1 hour in a 5% CO ₂ incubator at 37 ° C, followed by reaction with 0.1 M Na ₂ CO ₃ / NaHCO ₃ . To stop. Absorbance was measured at 405 nm using an ELISA reader.

Antiallergic Evaluation Results of Plant Blend Extracts sample Final concentration of the sample in the reaction solution (µg / ml) β- hexosaminidase % Inhibition of release Example 1. 200 21.3 Example 2. 200 15.3 Example 3. 200 17.7 Example 4. 200 25.7 Example 5. 200 39.3 Example 6. 200 27.1 Example 7. 200 29.8

Experimental Example  7. Corneometer CM  With 825 Moisturizing  Measure

The moisturizing effect of the plant mixture extract was measured using a Corneometer CM 825 by Courage + Khazaka Co., Ltd., and each extraction solvent was used as a control.

For each 5-10-year-old (average 7-year-old) young child per sample, the upper arm is divided into 2 × 2 ㎠ and 8 compartments. After 15 minutes, the samples were applied evenly by dropping 10 μl into each of the two compartments. After 2 hours, five measurements were taken per compartment using a Corneometer. Twice daily, twice a week, for 4 weeks. The experiment was conducted in a constant temperature and humidity room with a room temperature of 21 ± 2 ° C and a relative humidity of 50 ± 5%. The measured value was shown by averaging the value obtained by measuring five times about ten subjects.

The values range from 60 for people with normal to normal skin and 50-60 for dry.

Before application, a value of 55 (mean value) was shown.

Calculation method

① Corneometer Value = S-ΔS

S: Corneometer Value,

ΔS: Corneometer Value 2 hours after sample application

② Moisturizing power increase = Experimental moisturizing power-control moisturizing power

Moisturizing evaluation result of plant mixture extract Extraction solvent Measured value after sample application Increased moisture Example 1 Distilled water 54 0 Example 2 Ethyl alcohol 56 One Example 3 30% hydrous ethyl alcohol 58 3 Example 4 Butylene glycol 61 6 Example 5 30% water butylene glycol 65 10 Example 6 Propylene glycol 62 7 Example 7 30% water propylene glycol 59 4

As a result of measuring the moisture content of the skin using a corneometer, the dry skin value was 55 before application, but the value was higher when the value of normal skin after the application was over 60, and 30% hydrous butylene glycol was applied. Showed that the moisturizing effect was good.

Prescription Example  One.

A prescription example of a lotion (skin lotion) in a cosmetic containing an antistress complex extract is as follows.

Green tea (4), pine needles (2), licorice (2), bark skin (2), golden (3), red ginseng (2), soybeans (1), aloe (1), lettuce (1), persimmon leaf (2) ), Kiwi (1), sewage (2), camellia (2), and ginseng (4) of the plant extract extracted with 30% hydrous butylene glycol is called antistress complex extract.

number Raw material Content (% by weight) One Antistress Complex Extract 3.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Remaining amount Sum 100

Prescription Example  2.

Prescription example of nutrition lotion in cosmetics containing antistress complex extract is as follows.

number Raw material Prescription Example 2 Prescription Example 2-1 Comparative Prescription 2 One Antistress Complex Extract 3.0 6.0 - 2 Beeswax 1.0 1.0 1.0 3 Polysorbate 60 1.5 1.5 1.5 4 Solbitan Sesquioleate 0.5 0.5 0.5 5 Floating paraffin 10.0 10.0 10.0 6 Sorbitan stearate 1.0 1.0 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 0.5 0.5 8 Stearic acid 1.5 1.5 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 1.0 10 Propylene glycol 3.0 3.0 3.0 11 Carboxy Polymer 0.1 0.1 0.1 12 Triethanolamine 0.2 0.2 0.2 13 antiseptic a very small amount a very small amount a very small amount 14 Pigment a very small amount a very small amount a very small amount 15 Spices a very small amount a very small amount a very small amount 16 Purified water Remaining amount Remaining amount Remaining amount 17 water - - 10 Sum 100 100 100

Prescription Example  3

Prescription examples of nutritional creams in cosmetics containing antistress complex extracts are as follows.

number Raw material Prescription Example 3 Prescription Example 3-1 Comparative Prescription 3 One Antistress Complex Extract 5.0 10.0 - 2 Stearic acid 2.0 2.0 2.0 3 Cetyl alcohol 2.0 2.0 2.0 4 Glyceryl Monostearate 2.0 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 1.0 1.0 8 Wax 1.0 1.0 1.0 9 Liquid paraffin 4.0 4.0 4.0 10 Squalane 4.0 4.0 4.0 11 Caprylic / Capric Triglycerides 4.0 4.0 4.0 12 Carboxy Vinyl Polymer 0.3 0.3 0.3 13 Butylene glycol 5.0 5.0 5.0 14 glycerin 3.0 3.0 3.0 15 Triethanolamine 0.5 0.5 0.5 16 Purified water Remaining amount Remaining amount Remaining amount Sum 100 100 100

Prescription Example  4.

Prescription example of the essence of the cosmetic containing the anti-stress complex extract is as follows.

number Raw material Content (% by weight) One Antistress Complex Extract 5.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount Sum 100

apply Experimental Example  One. Primary skin  Efficacy of erythema after stimulation ( Closed Patch Experiment )

In order to confirm the stimulating alleviation effect when applying skin, add 0.5% Sodium Laurly Sulfate, a skin stimulant, to the nutritional creams of Formulations 3, 3-1 and Comparative Example 3 The experiment was conducted. 20 healthy boys, girls, and children aged 3 or older (age range: 3-8 years, average age: 6 years, male 15 years old and older) who have no history of or history of atopic dermatitis and other eczema, and have no current skin disease. , 5 women).

Measure the erythema index (E-index) before applying the sample to the inside of the forearm of both sides of the subject. The patch was attached during. After removing the patch, the subject exposed the test site in a space without air movement and no direct sunlight, and then stabilized for 30 minutes, and then measured the erythema index. After the measurement using a dermaspectometer (Dermaspectometer; Cortex Technology, Hadsund, Denmark) the results are shown as follows.

The room temperature was 22 ° C. and the humidity was 43%.

Skin primary irritation relief test results sample BL D0 D1 D2 W1   compare Prescription Example  3 9.6 13.5 12.1 12.3 11.1 Prescription Example  3 9.9 12.0 10.9 11.4 10.3 Prescription Example  3-1 9.7 11.3 10.1 10.1 10

BL: before sample application, D0: after sample treatment, D1: one day after sample treatment, D2: two days after sample treatment, W1; 1 week after sample processing

As a result of the investigation, it was found that the cosmetic composition of Formulation Example 3 prepared by adding the anti-stress complex extract was superior in stimulating stress relaxation effect than Comparative Formulation Example 3 prepared without addition.

apply Experimental Example  2. For skin sensitization  Contact hypersensitivity contact  hypersensitivity) inhibitory effect

The lotions of Formulation Examples 2 and 2-1 and Comparative Formula 2 were used in the test to determine the effect of inhibiting contact hypersensitivity by skin sensitization.

The abdomen of 8-10-week-old C57BL / 6 mice, which had undergone a one-week acclimation period, were epilated and irradiated with UV B 2.4KJ / ㎡ using an FS-20 sun lamp. It was observed to be inhibited. The lotions of Formulations 2 and 2-1 and Comparative Formula 2 were applied to the mouse abdomen after UV irradiation, and after 3 days, sensitized with 3% trinitrochlorobenzene in the UV irradiation area. After 6 days, 1% trinitrochlorobenzene was applied to the ears of mice, and the swelling state was measured.

sample Immune enhancing effect (%) compare Prescription Example  2 4.3 Prescription Example  2 18.3 Prescription Example 2-1 27.9

When the lotion of Formulation Example 2 and 2-1 and Comparative Example 2 were prepared and tested for hypersensitivity due to skin sensitization, the results showed that when Formulation Examples 2 and 2-1 were applied, the secondary skin after the first stimulation The effect of sensitization on skin irritation, erythema, and edema was effectively reduced, suggesting that the immune enhancing effect was improved, showing skin protection.

apply Experimental Example  3. Vapometer Percutaneous moisture evaporation using TEWL , trans epidermal  water loss ) Measure

In case of applying the lotion of Formulation Example 2, 2-1 and Comparative Example 2, the following clinical confirmation experiment was conducted to confirm the effect of preventing the percutaneous water loss caused by the external environment. 20 healthy males, females, and children over the age of 5 who do not have a history of or history of atopic dermatitis and other eczema and who do not currently have a skin disease (age distribution: 5-10 years, average age: 6 years, 7 men) , 13 women). Trans epidermal water loss (TEWL) was measured using VapoMeter (SWL-3002, Delfin) inside the arm. 20 μl of each sample was applied in the 2 × 2 cm 2 area inside the arm. All experiments were carried out three times in a clean room with a temperature of 25 ° C. and a humidity of 35.5% and measured at 1 hour intervals for 6 hours. Table 13 shows the value measured 6 hours after the last time, and the smaller the value, the better the moisturizing effect.

TEWL (g / m ² h) Sample time One hr 2 hr 3 hr 4 hr 5 hr 6 hr compare Prescription Example  2 7.9 8.2 8.5 8.8 8.3 8.1 Prescription Example  2 6.4 6.3 5.8 6.0 5.7 5.6 Prescription Example  2-1 5.5 5.3 5.3 5.4 5.2 5.1

As a result of measuring transdermal moisture evaporation using VapoMeter (SWL-3002, Delfin Co., Ltd.), the TEWL values of Formulation Examples 2 and 2-1 were low compared to the comparative prescriptions after 1 hour. It was found that the amount of moisture retention increased. In addition, it was found that the amount of moisture loss also decreases over time, indicating a good moisturizing effect.

The composition comprising the mixed plant extract according to the present invention can be widely used for skin protection as a substance having an excellent effect of protecting the skin from stresses derived from harmful environments. In particular, such a skin protection composition is a complex of natural plant extracts can be used in a variety of cosmetics, in particular infant-only cosmetics for the purpose of improving the skin to ensure a variety of efficacy and stability to the cells and human skin.

Claims (11)

It has skin protection effect against free radicals, dry skin and allergy containing plant mixed extract including green tea, pine needles, licorice, bark skin, golden, red ginseng, soybean, aloe, lettuce, red persimmon leaf, kiwi, sewage, camellia and ginseng Cosmetic composition. According to claim 1, wherein the plant mixture extract is based on dry weight green tea 10-15%, pine needles 5-10%, licorice 5-10%, bark 5-10%, golden 8-12%, goose 5-10 %, 1 ~ 5% soybean, 1 ~ 5% aloe, 1 ~ 5% lettuce, 5 ~ 10% persimmon leaf, 5 ~ 10% kiwi, 5 ~ 10% sewage, 5 ~ 10% camellia, and 10 ~ 15% ginseng Cosmetic composition comprising a. The method of claim 1, wherein the plant mixture extract is a mixture of the plants at 10 to 37 ℃ water, or anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene Cosmetic composition, characterized in that extracted for 1 to 15 days by immersion in at least one organic solvent selected from the group consisting of glycol and propylene glycol. The method of claim 1, wherein the plant mixture extract is water or one selected from the group consisting of anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol and propylene glycol Cosmetic composition characterized by the above-mentioned organic solvent was added and extracted by heating at 50-100 degreeC for 3 to 20 hours. The solvent of claim 1 wherein the solvent used for extraction is water or anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol and propylene glycol. Cosmetic composition, characterized in that at least one organic solvent selected. delete The cosmetic composition according to any one of claims 1 to 5, which is a skin lotion (skin lotion), nutrition lotion, nutrition cream, massage cream or nutrition essence. The cosmetic composition according to any one of claims 1 to 5, which is a baby lotion (skin lotion), a lotion, a cream, a sunscreen lotion or a sunscreen cream. The cosmetic composition according to any one of claims 1 to 5, which is a bath cleaner, a shampoo or a soap for infants. The cosmetic composition according to any one of claims 1 to 5, wherein the plant mixture extract is contained in its dry weight in an amount of 0.01-10.0% (w / v). The cosmetic composition according to claim 10, wherein the plant mixture extract is contained in an amount of 0.1-5.0% (w / v) as dry weight thereof.