KR102614182B1 - Cosmetic Compositions Comprising Extract of Zea mays husk - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles containing corn ( Zea mays ) husk extract as an active ingredient.
Because the extract provided by the present invention has excellent collagen production efficacy, it can be used as a composition for improving skin wrinkles.

Description

Cosmetic composition containing corn husk extract as an active ingredient {Cosmetic Compositions Comprising Extract of Zea mays husk}

The present invention relates to a cosmetic composition for improving wrinkles containing corn ( Zea mays ) husk extract as an active ingredient.

Collagen is a major matrix protein produced by fibroblasts of the skin and exists in the extracellular matrix. Its important functions include mechanical rigidity of the skin, connective tissue resistance and tissue adhesion, support for cell adhesion, and cell division and differentiation (organism). It is known to induce growth or wound healing. This collagen decreases due to age and photoaging caused by ultraviolet irradiation, and this is known to be closely related to the formation of wrinkles in the skin. Additionally, as extensive research on skin aging has developed in recent years, the important functions of collagen in the skin have been revealed. There are known active ingredients that promote collagen synthesis and have a wrinkle-improving effect. For example, retinoic acid, transforming growth factor (TGF), protein derived from animal placenta, betulinic acid, chlorella extract, etc. are known as substances that promote collagen synthesis. However, the amount of the active ingredients is limited due to safety issues such as irritation and redness when applied to the skin, or the effect is so minimal that the effect of improving skin function by promoting collagen synthesis in the skin cannot be expected.

Meanwhile, corn ( Zea mays ) is an annual herb, belongs to the monocot plant family Poaceae, and is one of the world's three major food crops along with wheat and rice. Corn grown and distributed domestically can be broadly classified into waxy corn, sweet corn, super-sweet corn, and fried corn. The waxy corn kernels are milky white and translucent, and are mostly used for steaming. Sweet corn is generally divided into sweet corn and sweet corn. Sweet corn has smaller and flatter kernels than sweet corn, and contains a large amount of sugar and moisture, so when dried, it becomes more wrinkled than sweet corn. Sweet corn is not only high in sugar but also low in fiber, so it can be boiled as a snack, eaten raw like fruit, or used in processed foods such as canned foods. Fried corn is often used as a snack because when the eggs are heated, the moisture in the center expands and they are easily fried to about 30 times their original volume.

The calories of corn are 106 kcal per 100g. In addition to moisture (about 64%), corn contains carbohydrates (29.4%), proteins (8.9%), lipids, minerals, and vitamins. Corn germ is rich in linoleic acid, an essential fatty acid. Corn is rich in vitamins B1, B2, E, and minerals such as potassium and iron, and contains a large amount of dietary fiber, which helps with dieting and preventing constipation. Vitamin E is good for preventing aging, increasing skin resistance, preventing cardiovascular disease, cancer, and increasing immunity, and vitamin B helps overcome summer lethargy. Corn germ is rich in linoleic acid, an essential fatty acid, which lowers cholesterol and helps prevent arteriosclerosis. The silky thread-like style connected to the ovary is corn silk, which has a diuretic effect and reduces swelling, so it is widely used as tea. Research results have also been published showing that maycin-based substances contained in corn silk extract are effective in improving and preventing vascular diseases by reducing blood cholesterol and inhibiting the gene expression of enzymes that synthesize cholesterol in the liver.

Cosmetic composition technologies using corn include, in Korea, Republic of Korea Patent Publication No. 10-2247965 “Cosmetic composition for skin whitening containing fermented corn extract” and Korea Patent Publication No. 10-1895969 “Containing corn cob extract.” In Korea, “Anti-inflammatory Composition for Moisturizing the Skin,” and in Korea, Republic of Korea Patent Publication No. 10-2072346, “Cosmetic Composition Containing Fermented Corn Extract for Moisturizing the Skin.”

In addition, in 2008, the present inventors used corn bran, i.e., the pericarp part (see Figure 1), which is the shell surrounding the corn kernels (see FIG. 1), to produce corn bran extract in Korean Patent Publication No. 10-1012118. A patent has been applied for “Cosmetic composition for improving skin wrinkles containing.”

While continuously researching not only the corn bran but also the efficacy of each part of corn, an extract was extracted from the corn husk area (Figure 2), which is the outer husk surrounding the corn stalk with corn kernels attached, which is a discarded by-product. It was confirmed that this collagen production effect exists.

Accordingly, the present inventors discovered that corn ( Zea mays ) hull extract exhibits excellent collagen production efficacy and can provide an effect of improving skin wrinkles, and thus completed the present invention.

Republic of Korea Patent Publication No. 10-2247965 Republic of Korea Patent Publication No. 10-1895969 Republic of Korea Patent Publication No. 10-2072346 Republic of Korea Patent Publication No. 10-1012118

The purpose of the present invention is to provide a cosmetic composition derived from natural products for improving wrinkles.

Another object of the present invention is to provide cosmetics containing the composition.

In order to achieve the above object, the present invention provides a cosmetic composition for improving wrinkles containing corn ( Zea mays ) husk extract as an active ingredient.

In the present invention, the extract is characterized in that it is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof.

In the present invention, the extract is characterized in that it includes a fraction of the extract.

In the present invention, the extract is characterized in that it includes an enzyme-treated extract.

In the present invention, the enzyme-treated extract is characterized in that it is obtained by treating the extract with a glycolytic enzyme.

In the present invention, the extract is characterized in that it includes a fraction of the enzyme-treated extract.

In the present invention, the extract is characterized in that it is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition.

In the present invention, the extract is characterized as having a collagen production effect.

Additionally, the present invention provides cosmetics containing the composition.

Because the extract provided by the present invention has excellent collagen production efficacy, it can be used as a composition for improving skin wrinkles.

Figure 1 is an image showing the names of each part of a corn kernel.
Figure 2 is an image showing the corn husk portion corresponding to the outer husk surrounding the corn stalk.

Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art.

Throughout the specification of the present application, when a part "includes" a certain component, this means that it may further include other components rather than excluding other components unless specifically stated to the contrary.

According to one embodiment of the present invention, the present invention relates to a cosmetic composition for improving wrinkles containing corn ( Zea mays ) husk extract as an active ingredient.

In the present invention, the corn husk corresponds to the outer husk area surrounding the corn stalk to which the corn kernels are attached.

The extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention pertains, and specifically, from the group consisting of water, lower alcohols with 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof. It may be extracted with a solvent of choice.

Specifically, the content of the extraction solvent may be 2 to 100 times the weight of corn ( Zea mays ) husk, more preferably 5 to 50 times, and most preferably 10 to 20 times.

Additionally, the extract can be extracted at room temperature or by heating under conditions where the active ingredients of the extract are not destroyed or destruction is minimized. The extraction method is not particularly limited, and depending on the type of extract and extraction method, ultrasonic extraction, cold needle extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction, etc. can be used.

Filtration is a process of removing floating solid particles from the extract. The particles may be filtered using cotton, nylon, paper, etc., or ultrafiltration, cryofiltration, centrifugation, etc. may be used, but are not limited thereto.

The step of concentrating and then drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, and infrared drying. In some cases, a process of grinding the final dried extract can be added.

According to one embodiment of the present invention, the extract can be processed and used in various forms such as fermentation broth, powder obtained by drying the extract, and concentrate obtained by concentrating and filtering the extract.

According to one embodiment of the present invention, the extract may include a fraction of the extract.

According to one embodiment of the present invention, the extract may include an enzyme-treated extract.

The enzyme-treated extract may be obtained by treating the extract with a glycolytic enzyme.

Additionally, the extract may include a fraction of an enzyme-treated extract.

According to one embodiment of the present invention, the corn ( Zea mays ) husk extract obtained from the above method may be included in an amount of 0.001 to 20% by weight, more preferably 0.1 to 1% by weight, based on the total weight of the composition. . If the extract is less than 0.001% by weight based on the total weight of the composition, the collagen production effect cannot be sufficiently obtained, so the composition's function is reduced, and if it exceeds 20% by weight, sufficient efficacy can be achieved even without containing more than that. Therefore, it is not only uneconomical, but it is also undesirable because the stability and spreadability of the formulation are reduced, making it difficult to use it as an external agent.

Since the extract can provide collagen production effect, it has the effect of fundamentally improving skin wrinkles.

As mentioned above, because the extract has excellent collagen production efficacy, it can be used as a cosmetic composition for improving skin wrinkles.

According to one embodiment of the present invention, when used as the cosmetic composition, ointment for external use, cream, softening lotion, astringent lotion, nourishing lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, spray , gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, eye cream, moisture cream, hand cream, foundation, nutritional essence, sunscreen, soap, It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion, and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, titanium oxide, etc. may be used as carrier ingredients. You can.

When the formulation of the present invention is a body gel, in addition to the above-mentioned active ingredients, ingredients generally used in the production of body gel, such as carbomer, triethanolamine, menthol, ethanol, glycerin, disodium EDTA, methylparaben, fragrance, etc. This can be used. Here, carbomer and triethanolamine act as thickeners, menthol acts as a fragrance ingredient, ethanol functions as a refreshing sensation, glycerin acts as a moisturizer, and disodium EDTA acts as a metal component chelator. It functions as a preservative, and methylparaben is added to function as a preservative. Additionally, in addition to the menthol scent, various scents such as herbal scent may be added to the scent.

In addition to the specific ingredients described above, it is also within the scope of the present invention to manufacture a composition by applying known substances widely used in the present technical field to achieve the functions of thickener, refreshing sensation, fragrance ingredient, moisturizer, etc. described above. May be included.

When the formulation of the present invention is a powder or spray (spray), lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be added as a carrier component. In particular, when the formulation is a spray, chlorofluorohydride may be added. May contain propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is added as a carrier component, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene glycol. , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, and sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide ether. Sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

According to one embodiment of the present invention, the cosmetic composition can be manufactured into a composition such as body gel, body lotion, body cream, body oil, etc. for promoting fat decomposition by adding common additives, and can also be manufactured in an aerosol type. You can. In this case, the cosmetic composition is preferably used by transdermal administration by applying or spraying it directly to the skin.

According to one embodiment of the present invention, the amount of the cosmetic composition used can be appropriately adjusted depending on individual differences such as age and level of skin fat, formulation, and form, and can be usefully used as a composition for improving skin wrinkles. there is.

The cosmetic composition obtained from the above method exhibits collagen production efficacy and can provide excellent wrinkle improvement cosmetics.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it is clear to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-explanatory.

<Examples 1 to 4> Preparation of corn husk extract

The raw material was prepared by separating and drying the husk of Chodang corn grown in Goesan. The weight of the corn husk was measured, 20 times the weight of 75% (v/v) ethanol aqueous solution was added, heated and extracted, and then filtered. The extraction was repeated twice, and the obtained extract was concentrated under reduced pressure to powder the extract to prepare a 75% (v/v) ethanol extract of corn husk (Example 1).

The ethanol extract of Example 1 was dissolved by adding distilled water and 10% (v/v) ethanol aqueous solution, and then fractionated and concentrated with ethyl acetate to obtain a cornhusk ethyl acetate fraction (Example 2).

Distilled water and 10% (v/v) ethanol aqueous solution were added to the ethanol extract of Example 1 and dissolved. Pectinex (Novozymes, Denmark), a glycolytic enzyme, was added thereto, reacted at 50-60°C for 30 hours, filtered, and concentrated under reduced pressure at 40°C to obtain an enzyme-treated extract (Example 3).

The enzyme-treated extract of Example 3 was dissolved by adding distilled water and 10% (v/v) ethanol aqueous solution, and then fractionated and concentrated with ethyl acetate to obtain an ethyl acetate fraction (Example 4).

<Comparative Examples 1 to 3> Preparation of corn bran, corn silk, and corn grain extract

The raw material was prepared by separating dried and powdered corn bran from domestically cultivated maize and corn grown in Goesan into silk and kernel parts and drying them. The weight of each of the corn bran, corn silk, and corn grains was measured, and 20 times the weight of a 75% (v/v) ethanol aqueous solution was added, heated, extracted, and then filtered. The above extraction was repeated twice, and the obtained extract was concentrated under reduced pressure to powder the extract to produce 75% (v/v) ethanol extract of corn bran (Comparative Example 1) and 75% (v/v) ethanol extract of corn silk (Comparative Example). 2) and 75% (v/v) ethanol extract of corn kernels (Comparative Example 3) was obtained.

<Experimental Example 1> Cytotoxicity test: MTT assay

To confirm the cytotoxic effect using the extracts from each part of corn in Examples 1 to 4 and Comparative Examples 1 to 3, a cytotoxicity experiment was conducted using fibroblasts (HS68 cells).

Human-derived fibroblasts (HS68 cells) were purchased from ATCC (American Type Culture Collection) and DMEM medium (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) containing 10% FBS (fetal bovine serum, Gibco BRL, USA). was cultured at 37°C and 5% CO ₂ . Cultured HS68 cells were distributed at 1x10 ⁴ cells/mL per well in a 96-well plate and cultured for 24 hours. After culturing, each extract was replaced with serum-free DMEM medium diluted by concentration and cultured for 24 hours. After 24 hours, 1/10 of the CCK-8 (Cell Counting Kit, Dojindo, Japan) solution was added to the cell culture medium and incubated at 37°C for 2 hours, and then the absorbance was measured at 450 nm using a microplate reader. . The experiment was performed three times each, expressed as the average value ± standard deviation, and calculated using Equation 1 below.

[Equation 1]

Cell viability (%)=100-(absorbance of each sample/absorbance of control) x 100

division Cell survival rate (%) 0 μg/mL 10 μg/mL 20 μg/mL 50 μg/mL 100 μg/mL Example 1 100.0 99.2 ± 1.5 98.4 ± 1.8 99.6 ± 0.8 99.1 ± 1.9 Example 2 100.0 97.6 ± 0.9 106.5 ± 2.9 119.0 ± 5.7 95.9 ± 3.7 Example 3 100.0 95.7 ± 3.9 98.8 ± 2.5 98.7 ± 0.9 97.8 ± 2.6 Example 4 100.0 106.2 ± 1.7 102.1 ± 2.0 117.7 ± 4.4 124.7 ± 2.6 Comparative Example 1 100.0 100.1 ± 1.8 98.5 ± 1.0 1056 ± 0.9 99.8 ± 0.5 Comparative Example 2 100.0 98.8 ± 2.4 100.9 ± 2.3 99.4 ± 1.7 98.9 ± 1.0 Comparative Example 3 100.0 96.7 ± 1.6 98.6 ± 0.7 97.5 ± 2.5 98.2 ± 2.3

As a result, as shown in Table 1, no cytotoxicity was observed up to a concentration of 100 μg/mL in Examples 1, 3, and 4 and Comparative Examples 1 to 3, and in Example 2, cells were observed at a concentration of 100 μg/mL or more. influence was observed. Therefore, Examples 1, 3, 4 and Comparative Examples 1 to 3 were conducted at a maximum concentration of 100 μg/mL, and Example 2 was conducted at a maximum concentration of 50 μg/mL.

<Experimental Example 2> Wrinkle improvement efficacy test: Measurement of collagen production effect

The collagen production effect was measured to determine the wrinkle improvement effect using extracts from each corn part of Examples 1 to 4 and Comparative Examples 1 to 3.

First, cultured fibroblast (HS68 cells) cells were dispensed at 1x10 ⁵ cells/mL per well in a 24-well plate and cultured for 24 hours. After culturing, based on the results of Experimental Example 1, the medium was replaced with serum-free DMEM medium diluted to a concentration of 100 μg/mL for Examples 1, 3, and 4 and Comparative Examples 1 to 3 and 50 μg/mL for Example 2. Cultured for 24 hours. The amount of collagen synthesis was measured by measuring the amount of procollagen type I C-peptide (PICP) using a collagen measurement kit (Procollagen Type IC peptide EIA kit, Takara, Japan). The absorbance of the cell culture medium without any sample treatment was set to 100% of the control group, and the experiment was performed three times each and expressed as the average value ± standard deviation.

division Collagen production effect (%) control group 100.0 Example 1 138.6 ± 19.2 Example 2 150.2 ± 5.9 Example 3 179.9 ± 3.6 Example 4 123.6 ± 6.5 Comparative Example 1 125.1 ± 2.7 Comparative Example 2 122.4 ± 23.1 Comparative Example 3 143.8 ± 20.2 L-ascorbic acid (positive control) 170.8 ± 22.2

As a result, as can be seen in Table 2, all of Examples 1 to 4 had excellent collagen production effects, but among them, Examples 2 and 3 showed a better collagen production effect, confirming that the wrinkle improvement effect was significantly higher. In addition, a collagen production effect was also observed in Comparative Examples 1 to 3, but a significant collagen production effect was not observed in Comparative Example 2.

<Experimental Example 3> Evaluation of skin wrinkle improvement effect and skin irritation

In order to evaluate the skin wrinkle improvement effect and skin irritation, an experiment was conducted on the corn husk extract of Example 3, which had excellent efficacy, based on the evaluation results of the above experimental examples. The composition containing the corn husk extract of Example 3 was a cream-type composition prepared by a conventional manufacturing method as shown in Table 3 below, and a sensory test was conducted using this composition.

ingredient Comparative formulation example (% by weight) Formulation example (% by weight) One Cetearyl alcohol 1.0 1.0 2 Glyceryl Stearate/PEG-100 Stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 squalane 8.0 8.0 7 Africode Kernel Oil 4.0 4.0 8 Dimethicone 2.0 2.0 9 glycerin 5.0 5.0 10 Magnesium Aluminum Silicate 0.4 0.4 11 xanthan gum 0.03 0.03 12 Preservatives and Fragrances a very small amount a very small amount 13 Purified water (to 100) remaining amount remaining amount 14 Cornhusk extract of Example 3 - 5.0

First, 15 women aged 20 years or older were given a cream containing the corn husk extract of Example 3 (test group) on the left part of their faces, and a cream not containing the corn husk extract of Example 3 (control group) on the right part of their faces. was used continuously for 12 weeks, twice a day. The sensory test evaluated the wrinkle-improving effect relative to the wrinkle-improving effect on the skin, and the sensory evaluation of skin irritation evaluated phenomena such as itching, stinging, and erythema on the skin. The evaluation was conducted based on the five-point method criteria of very good (5 points), excellent (4 points), average (3 points), poor (2 points), and very poor (1 point), and the results are as follows.

number Wrinkle improvement effect skin irritation Formulation example Comparative formulation example Formulation example Comparative formulation example One 5 4 5 4 2 3 4 4 5 3 4 4 4 5 4 5 5 5 4 5 5 3 5 5 6 4 4 4 5 7 3 4 5 5 8 4 4 4 4 9 4 3 4 5 10 4 4 5 4 11 5 4 5 4 12 4 3 5 4 13 4 5 5 5 14 5 3 5 5 15 4 4 4 4 average 4.20 3.87 4.60 4.53

As a result, as can be seen in Table 4, the formulation example had a lower degree of skin irritation compared to the comparative formulation example, showing excellent skin safety and superior wrinkle improvement efficacy.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

A cosmetic composition for improving wrinkles comprising, as an active ingredient, an extract obtained by extracting corn ( Zea mays ) husk with ethanol and then treating it with a glycolytic enzyme.
delete delete delete delete delete The method of claim 1, wherein the extract contains as an active ingredient an extract obtained by extracting corn ( Zea mays ) husk with ethanol and then treating it with a glycolytic enzyme, wherein the extract contains 0.001 to 20% by weight based on the total weight of the composition. Cosmetic composition for improving wrinkles.
The cosmetic composition for improving wrinkles according to claim 1, wherein the extract contains as an active ingredient an extract obtained by extracting corn ( Zea mays ) husk with ethanol and then treating it with a glycolytic enzyme, which has a collagen-producing effect.
A cosmetic comprising the composition of any one of claims 1, 7, and 8.