KR100729833B1 - A composition for improving wrinkle on skin comprising plant extracts - Google Patents

Abstract

A composition for improving wrinkle on the skin comprising plant extracts is provided to accelerate biosynthesis of collagen in the skin without causing skin troubles such as irritation and flare, thereby removing skin wrinkles. The composition for improving wrinkle on the skin comprises 0.0001 to 10.0 wt.%, preferably 0.0001 to 5.0 wt.%, more preferably 0.0001 to 1 wt.% of the extract of maize(Zea mays) leaf as an effective ingredient, wherein the maize(Zea mays) leaf extract is prepared by extracting powder of maize(Zea mays) leaf with water, C1-C4 anhydrous or hydrous lower alcohol, ethylacetate, acetone or hexane, preferably ethanol at 40-100 deg.C for 1-5 hours. The composition is a cosmetic, food or pharmaceutical composition.

Description

A composition for improving Wrinkle on Skin Comprising Plant Extracts}

1 is a graph showing the collagen biosynthesis promoting effect by the plant extract of the present invention ((-) control group: untreated group, RA: 1 uM retinoic acid).

Figure 2 is a picture showing the cytotoxic effect on the plant extract of the present invention ((-) control: untreated group, RA: 1 uM retinoic acid).

The present invention relates to a composition for improving skin wrinkles comprising a plant extract.

In general, skin wrinkles are known to be produced by a number of factors, including skin moisture content, collagen content, and the ability to act on the environment. The greatest influence on the formation of double wrinkles is the amount of collagen produced.

Collagen is a major matrix protein produced in the fibroblasts of the skin and is present in extracellular epilepsy and accounts for 30% of the total weight of biological proteins and has a spiral structure. Its main function is known as a major ingredient that gives skin elasticity through functions such as mechanical firmness of skin, resistance of connective tissue and binding of tissue, and support of cell adhesion. Not only does collagen content decrease due to internal factors such as decreased cellular activity due to natural aging, but also collagen biosynthesis decreases due to external factors such as increased stress caused by various harmful environments and increased free radical species caused by sunlight. There is a problem that collagen decomposition is increased. Overcoming these problems, efforts to improve skin wrinkles have been steadily attempted.

Retinoic acid, TGF-beta, protein from animal placenta (JP8-231370), betulinic acid (JP8-208424), chlorella extract (JP40523), yul extract ( KR-10-1997-0058952) and the like are known. However, retinoic acid is a very unstable substance, which is sensitive to light (ultraviolet rays), moisture, heat, and air (oxygen), and easily causes chemical changes, as well as causing skin problems such as irritation and redness when applied to the skin. Reported. Therefore, there is an urgent need for the development of new collagen synthesis promoters having high safety and stability in vivo and high collagen synthesis rate.

The present inventors screened collagen production promoting material for about 100 kinds of Jeju native plants. As a result of the screening, a number of plant extracts have completed the invention by confirming that collagen biosynthesis may promote collagen biosynthesis, have an excellent effect on improving skin wrinkles, and may be an effective ingredient of a composition for improving skin wrinkles.

One object of the present invention is to provide a wrinkle improvement composition comprising a plant extract newly identified according to the present invention.

Still another object of the present invention is to provide cosmetics, foods and / or pharmaceuticals for wrinkle improvement made of the above composition.

In one embodiment, the present invention is a forsythia extract, fern extract, arborella extract, buttercup extract, peppermint extract, leek extract, sesame leaf extract, Youngsan Hong flower extract, cucumber grass root extract, corn leaf extract, pea extract, rapeseed extract, It relates to a composition for improving wrinkles comprising at least one extract selected from pogongyoung extract.

The present inventors measured about 100 plant extracts for promoting collagen biosynthesis effect used in the screening of wrinkle improvement substances. As a result, it was found that the aforementioned extracts exhibited a phenomenon of promoting collagen production in a concentration-dependent manner.

Forsythia (forsythia koreana ) is a deciduous shrub belonging to the ash family, also used for medicinal purposes. Fruit is used as a medicine, the herbal medicine name is Fengyo or Wang Sudan, and it is effective for antipyretic, detoxification, anti-inflammatory, diuretic and small type. Preferred sites for use according to the invention are flowers and stems.

Fern ( Pteridium aquilinum var.latiusculum ) is a perennial herb of fern family, boiled young leafless sprouts in spring and eat as herbs or as a ingredient of soup, and in Chinese medicine, young sprouts are used as medicinal herbs. It is known to have a mild diuretic effect. Preferred sites for use according to the invention are stems and roots.

Aralia elata is a deciduous broad-leaved shrub of the family Elderaceae , sprouted with edible shoots, dried bark of herbal medicines, dried fruit and roots in oriental medicine, used in seawater, stomach cancer, diabetes, digestive agents. Bark or root to eat diabetes. Preferred sites for use according to the invention are stems and roots.

Buttercup ( Oenanthe javanica (Bl.) DC.) Is a perennial herb. Carrots are effective in clearing heat and water. It is used to treat swollen buns, jaundice, edema, disease, lobster, mumps, rheumatic neuralgia, and myositis. Preferred sites for use according to the invention are leaves, stems and roots.

Peppermint ( Mentha arvensis var. Piperascens ) is a perennial herb of dicotyledon and moss plant. I use it as a refreshing agent. Preferred sites for use according to the invention are leaves and stems.

Leek ( Allium tuberosum ) is a perennial herb of liliaceae family, also known as tennis, sorghum, weed and weed, and scaly stems are used for dry stomach, suit and burn, and seeds are used as herbal medicine for urinary tract. , Soft plants are edible. The sites used according to the invention are leaves and stems.

Houttuynia cordata is a perennial plant of the dicotyledonous plant, which is a tricot herbaceous perennial plant. It uses plants before flowering as diuretics and insect repellents, and when the leaves are crushed and bitten by boils and poisons. In the private sector, it is used for boils, purulent and hemorrhoids, and in oriental medicine, plants are used for gonorrhea, enteritis, urinary tract infections, pneumonia, and bronchitis. Preferred sites for use according to the invention are leaves, stems and roots.

Young Sanhong ( Rhododendron indicum ) is an evergreen broad-leaved shrub of the genus Ericaceae, whose flowers are used in the present invention.

Cucumber ( Sanguisorba officinalis Linne ) belongs to the Rosaceae. Cucumbers have been used for diarrhea, colitis, bleeding, swelling, and burns. In particular, hemostatic action is strong and is used a lot of blood to bleed various kinds of bleeding. There are many similar plants such as wild cucumber, thin leaf cucumber, and big cucumber, and they show the same effect. Preferred sites for use in the present invention are roots.

Corn (maize: Zea mays ) is an annual herb from a monocotyledonous rice plant, whose fruits are harvested after ripening and used for food. It makes cookies, bread, dumplings, and porridge, and is also used for livestock feed. As an industrial raw material, it is used for corn starch, glucose, grass, and brewing in seed. Preferred sites for use according to the invention are corn leaves.

Peas ( Pisum sativum L. ) is a legume growing month, and young sprouts and berries are eaten. Seeds are called peas and are medicinal. Seeds contain phytoaglutinin, abcicin II, and gibberellin A20, and immature seeds contain 4-chloroindolyl-3-acetyl-aspartic acid monomethyl esters. The two families contain gibberellin. It is easy to ingestion, summer, and urine and has the effect of resolving sores. It is used to treat kwakran, jeoneungi, each, carbuncle. Preferred sites for use according to the invention are the leaf and stem portions.

Rapeseed ( Brassica campestris subsp.napus var.nippo-oleifera Makino ) is a biennial herb in Cruciferaceae, oiled with seeds, medicinal roots and leaves are medicinal. The pandemic has the effect of acid blood, swelling. Treat postpartum blood and fish blood, hearth, hemostasis, hemolysis, solitary, heat poisoning, spear, oozing. The pandemic has the effects of haemostasis, destruction, seedling, and sedimentation. Treat postpartum abdominal pain, bleeding, blood clots, poison, chiru, and dreams. Preferred sites for use according to the invention are flowers and stems.

Sasa borealis (Hack.) Makino is an evergreen houseplant of rice plants and uses leaves for dentition. Preferred sites for use according to the invention are the leaf and stem portions.

Pogongyoung (Mongolian dandelion, Taraxacum platycarpum) is a perennial herb of the Asteraceae family, also known as dandelion, ophthalmic room, sitting room, snotweed, blotch, and pogongcho. In spring, young leaves are eaten as herbs and herbal medicine is used as a medicine called pogongyoung before flowering. It is effective for swelling, mastitis, sore throat, appendicitis, peritonitis, acute hepatitis and jaundice due to fever. use. It can also be used as a drug to make milk faster. Preferred sites for use according to the invention are the flower and stem parts.

The plant extract included in the composition of the present invention can be extracted from each plant using water, an organic solvent, or a mixed solvent thereof. All of these components may be mixed and extracted at the same time, or individual components may be extracted and mixed under optimum conditions. In the case of hot water extraction, for example, the components of the composition of the present invention are added to water and heated at a temperature of at least 70 ° C, preferably at least 80 ° C, more preferably at least 90 ° C, for at least 2 hours, preferably at least 3 hours, The heated mixture can be prepared by filtration. When heating at a high temperature, the time is reduced, but the active ingredient is destroyed and can be lost. When heating at a temperature lower than the temperature, it is important to control the temperature and time properly because the active ingredient of the mixture is not completely extracted. This extract can be dried or used as it is. When extracted using an organic solvent, alcohols such as methanol, ethanol, isoprotanol, butanol, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N-dimethylformamide), DMSO (dimethylsulfoxide) Side), mixtures thereof and the like can be extracted with an organic solvent at room temperature or by heating.

In one specific embodiment, the above-mentioned plants are collected and pulverized and refluxed with 5 to 20 volumes of water or 1 to 4 anhydrous or hydrous lower alcohols, ethyl acetate, acetone or hexane based on the dry weight of the mill. The extract may be heated at 40 to 100 ° C. for 1 to 5 hours, or deposited at room temperature for 1 to 30 days.

The extract extracted by heat extraction or cold extraction is filtered to remove suspended solid particles, for example, by filtering the particles using nylon or the like, or filtering using a freeze filtration method, and then using them as they are or using lyophilization, vacuum drying, and hot air. Drying, spray drying, etc. may be used for drying.

Each plant extract in the composition for improving skin wrinkles of the present invention is about 0.0001 to 10.0% by weight, preferably 0.0001 to 5.0% by weight, and more preferably 0.0001 to 1% by weight, based on the dry weight of the composition. It may be included as.

The composition of the present invention exhibited a facilitating activity of collagen synthesis in a dose-dependent manner (Experimental Example 1, FIG. 1) and did not show cytotoxicity in in vitro experiments (Experimental Example 2, FIG. 2). In addition, clinical trials showed an excellent skin elasticity improving effect (Experimental Example 3, Table 3), and was confirmed to be safe in cumulative stimulation experiments on human skin (Experimental Example 4, Table 5).

Plant extracts included in the composition of the present invention can be used as a single active ingredient, by using a combination of these plant extracts can also obtain a better wrinkle improvement effect. These plant extracts may be used as a main ingredient or as an additive for cosmetics, foods, medicines and the like for skin wrinkle improvement.

Thus, in another aspect, the present invention relates to a cosmetic, food or pharmaceutical preparation made with the above-mentioned plant extract.

When used in cosmetics, it can be prepared in any formulation conventionally prepared in the art, astringent lotion, flexible lotion, nourishing cream, essence, face wash, face wash, face wash, nourishing gel, massage cream, moisturizing cream It can be formulated in various forms such as, pack, body shampoo, hair shampoo, hair rinse, hand cream and foundation. In addition, when used in medicine, it may be formulated as an external skin ointment, patch, aerosol, etc., when used as a food, can be processed and used within the scope of ordinary knowledge in the art of functional food. will be.

In a specific specific embodiment, the present invention relates to a cosmetic comprising the above-mentioned plant extract. Especially preferably, it may be formulated as a nourishing cream, astringent lotion, a flexible lotion, an essence, a nourishing gel or a massage cream.

Cosmetics according to the present invention may be prepared according to methods known in the art. Ingredients included in the cosmetic composition of the present invention as an active ingredient may include components commonly used in cosmetic compositions in addition to the above-described plant extract, for example vitamins, peptides, polysaccharides, oils and fats, moisturizers, emollients, interfaces Active agents, organic and inorganic pigments, preservatives, fungicides, antioxidants, pH adjusting agents, alcohols, pigments, flavorings, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils. Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl esters, hydroxystearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like. As surfactant, a nonionic surfactant, anionic surfactant, cationic surfactant, an amphoteric surfactant, etc. are mentioned. Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid. Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like. Examples of the alcohol include higher alcohols such as cetyl alcohol.

When the formulation of the present invention is a paste, cream or gel, animal fats, vegetable fats, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

Hereinafter, the present invention will be described in detail by examples.

Preparation Example 1 Preparation of Plant Extract

Forsythia flowers and stems (Nanji Agricultural Research Institute), fern stems and roots (Nanji Agricultural Research Institute), arbor stems and roots (Nanji Agricultural Research Institute), leaves of buttercups, stems and roots (Jeju traditional market), mint leaves Korean stalk (Jeju traditional market), leek leaf and stalk (Jeju traditional market), Eoseongcho leaf, stem and root (Nanji Agricultural Research Institute), Youngsan Hongflower (Nanji Agricultural Research Institute), cucumber grass root (Jeju Traditional Market), corn leaf (Jeju Traditional Market), Pea Leaf and Stem (Jeju Traditional Market), Rapeseed Flower and Stem (Nanji Agricultural Research Institute), Pole Leaf and Stem (Nanji Agricultural Research Institute), Pogongyoung Flower and Stem (Nanji Agricultural Research Institute) It was collected, finely pulverized and extracted by immersion for 10 days at room temperature with 10 volumes of ethanol based on the dry weight of the pulverized product. After filtration with a filter cloth, the residue was further extracted one or more times in the same manner. The extracts were combined, concentrated under reduced pressure, and lyophilized to obtain a dry extract.

Example 1: Promoting Type I Procollagen Biosynthesis

Collagen (types I, II, III, IV and V) are synthesized in the form of precursors called procollagens. Procollagen includes peptide sequences called propeptides at the amino and carboxy termini. The function of the propeptide is known to assist in the folding of procollagen molecules in the endoplasmic reticulum and to be cleaved and separated from the collagen molecules when collagen polymerization occurs. Therefore, by measuring the amount of the isolated propeptide, it is possible to determine the degree of collagen biosynthesis in the cell.

First, human skin fibroblasts (Pacific) were seeded in a 6-well dish at a certain number of cells (1 × 10 ⁵ cells), incubated for one day, and then cultured in each well containing 0.05% of FBS in the preparation example. The prepared extract was treated by concentration, and then incubated again for 48 hours. After 48 hours, cell media were collected and the amount of type I procollagen in each sample collected was quantified with an ELISA leader at A _{450 nm} using an antibody against procollagen. Retinoic acid was used as a positive control.

All 16 native Jeju plant extracts prepared in Preparation Example 1 increased collagen production rate (FIG. 1).

Laboratory Example 2: Cytotoxicity

To test the cytotoxic effect on plant extracts using human normal fibroblast (Pacific) culture, MTT was cultured in serum-free state after sample treatment with extract prepared in Preparation Example. It was measured using the (3- (4,5-dimetylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) assay method. MTT is a pale yellow substrate that is cleaved by respiratory enzymes in the mitochondria of living cells and produces dark blue formazan. The reaction does not occur in dead cells, so the amount of formazan produced can be used to determine viable cell count. All 16 extracts (1 ug / ml, 10 ug / ml) were confirmed to have no cytotoxic effect in human normal fibroblasts (FIG. 2).

Experimental Example 3: Skin Wrinkle Improvement Effect in Human Body

In order to measure the antiwrinkle effect of the cosmetics containing Jeju native plant extracts prepared in the preparation examples, creams each containing the plant extracts were prepared according to the methods in the table below.

A. Preparation of Cream Containing Jeju Native Plant Extracts

Examples of preparation of nutrition creams containing Jeju native plant extracts are shown in Table 1 below. Purified water, triethanolamine, and propylene glycol, which were aqueous phases, were dissolved by heating at 70 ° C. The oily fatty acid, an oily component, an emulsifier, and the preservative were melt | dissolved by adding the liquid which heated and melted at 70 degreeC here. After the emulsification was completed, the solution was cooled to 45 ° C., added and dispersed asiaticoside and fragrance, and then cooled to 30 ° C.

ingredient Content (% by weight) Jeju native plant extracts (forsythia, fern, araliaceae, buttercup, peppermint, leek, eoseongcho, Youngsan red flower, cucumber grass root, corn leaf, pea, rapeseed, stalk or pogongyoung) 0.01 Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Disteare 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Remaining amount Sum 100

B. Preparation of Cream Containing Purified Water (Comparative Preparation)

Table 2 shows a preparation example of the cream replaced with purified water without adding Jeju native plant extracts in paragraph A.

ingredient Content (% by weight) Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Distearate 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Remaining amount Sum 100

Wrinkle improvement effect was evaluated by measuring the change of skin elasticity using the nourishing cream prepared in Preparation Example A (nutrition cream containing Jeju native plant extracts each 0.01%) and Preparation Example B (nutrition cream containing purified water). .

Skin elasticity was measured in 30 healthy women (25 to 35 years old) who were constantly maintained at a temperature of 24 to 26 ° C. and a humidity of 38 to 40%. After three months of use twice a day, measurements were made using a cutometer SEM 474. The criteria for comparison were relative values of 0 for no skin elasticity and 5 for many cases. The test results are shown in Table 3 below.

Test Items  Cream Preparation A (0.01% plant extract) Cream preparation example B forsythia Bracken Arbor Poongyoung Buttercup mint chives Eoseongcho Youngsan Red Flower Cucumber grass roots Corn Leaves pea Rapeseed Scoop Skin elasticity 3.2 3.1 3.0 2.8 2.9 2.9 3.3 3.1 2.9 2.9 3.2 3.1 2.9 3.0 1.60

As shown in Table 3, Preparation Example A of the present invention was found to be much better wrinkle improvement effect than the Comparative Preparation Example.

Laboratory example 4: safety check on human skin

4-1. Preparation of external skin preparations

In order to confirm whether Jeju native plant extracts, which were found to be excellent in wrinkle improvement as described above, are safe for human skin, external skin preparations containing Jeju native plant extracts were prepared and skin safety verification experiments were performed.

Skin external preparations containing Jeju native plant extracts were prepared with the ingredients in Table 4 below. In the following Table 4, the control group is a skin external preparation that does not include the wrinkle improvement component, and the test group is a skin external preparation including each plant extract.

For the preparation of external skin preparations, purified water, glycerin and butylene glycol were mixed and dissolved at 70 ° C. (aqueous part), and the other components except the above three components and trimethanolamine were dissolved at 70 ° C. (oil phase part). The oily part was added to the aqueous part and stirred first with a homomixer (Tokushu Kika, Japan), to which trimethanolamine was finally added. After removing the bubbles generated in the mixed solution, and cooled to room temperature to prepare a skin external preparation.

ingredient content (%) Control Test group Purified water 72 72 glycerin 8.0 8.0 Butylene glycol 4.0 4.0 Jeju native plant extract (forsythia, fern, arborella, buttercup, peppermint, leek, eoseongcho, young red flower, cucumber grass root, corn leaf, pea, rapeseed, stalk or pogongyoung) 0 1.0 Caprylic Capri Triglyceride 8.0 8.0 Squalane 5.0 5.0 Cetearyl Glucoside 1.5 1.5 Sorbitan stearate 0.4 0.4 Cetearyl Alcohol 1.0 1.0 Trimethanolamine 0.1 0.1 Gross weight 100 100

4-2. Skin accumulation stimulation test

Jeju native plant extracts irritate the skin by performing a total of nine 24-hour cumulative blisters on the upper forearm every other day for 30 healthy adults using each skin preparation prepared in Example 4-1. Was measured.

The patching method used a fin chamber (Finn chamber, Epitest Ltd, Finland). 15 ul of each said skin external preparation was dripped at the chamber, and the patch was performed. The degree of response to the skin each time was scored using the following equation, and the results are shown in Table 5 below.

Average responsiveness = [[response x responsiveness / total number of subjects x highest score (4 points)] x 100] ÷ number of tests (9)

At this time, ± 1 point, + 2 points, + + 4 points in the reactivity, it is determined as a safe composition when the average reactivity is less than 3.

Test substance Number of subjects with response Average reactivity 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + ㅁ + + + Control 2 - - --- --- --- --- --- --- --- --- 0.18 Forsythia extract 2 - - --- --- --- --- --- --- --- --- 0.18 Fern extract 2 - - --- --- --- --- --- --- --- --- 0.18 Elm extract 2 - - --- --- --- --- --- --- --- --- 0.18 Buttercup extract 2 - - --- --- --- --- --- --- --- --- 0.18 Peppermint Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Leek Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Eochocho extract 2 - - --- --- --- --- --- --- --- --- 0.18 Youngsan Hong Flower Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Cucumber Grass Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Corn Leaf Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Pea Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Rapeseed Extract 2 - - --- --- --- --- --- --- --- --- 0.18 Stalk extract 2 - - --- --- --- --- --- --- --- --- 0.18 Pogongyoung extract 2 - - --- --- --- --- --- --- --- --- 0.18 Inspector 30 30 30 30 30 30 30 30 30

In the test group as shown in Table 5, there was no particular side effect. When it calculated in accordance with the formula described above, since the average reactivity of all 14 kinds became 0.18 and became 3 or less, it was judged as a safe composition.

According to the present invention, 14 native Jeju plant extracts have a collagen biosynthesis effect similar to retinol, the main ingredient of skin wrinkle improvement. In addition, no toxicity or irritation occurred in the human safety test, which proved to be safe for human use. Therefore, the 14 native Jeju plants can be usefully used for cosmetics, medicine and foods for the purpose of wrinkle improvement.

Claims (3)

Cosmetic composition for improving skin wrinkles comprising corn leaf extract. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the corn leaf extract is corn leaf ethanol extract. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the content of the corn leaf extract is 0.0001 to 10.0 wt% in dry weight based on the total weight of the composition.

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