JP2003192605A - Lipase inhibitant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a lipase inhibitant capable of suppressing and preventing obesity and hyperlipidemia, and useful for the prevention and treatment of acne and dermatitis caused by a bacterial lipase and skin diseases such as dandruff. <P>SOLUTION: This lipase inhibitory agent contains at least 1 kind selected from a group consisting of plants of Arctos taphylos uvae urusi, Primula veris, Ficus religiosa, Asparagus officinalis, Ginkgo biloba, Jatropha macrantha, Solidago gigantea, Tilia europaea, Erythroxylum vaccinifolium, Eucaliptus globulus, Engelhardtia chrysolepis, Rhamnus purishiana, Ononis spinosa, Saraca indica, Polygala senega and a royal jelly. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a lipase inhibitor, and more specifically to obesity by effectively inhibiting pancreatic lipase, which is a key to obesity and hyperlipidemia, which is incapable of digesting and absorbing lipids in vivo. The present invention relates to a highly safe lipase inhibitor which can contribute to the suppression and prevention of hyperlipidemia and is effective in the prevention and treatment of skin diseases such as acne, dermatitis and dandruff caused by bacterial lipase.

[0002]

2. Description of the Related Art Conventionally, abnormal fat deposition in adipose tissue and various organs of the body, resulting in obesity, or hyperlipidemia showing abnormally high serum lipids is associated with hypertension, arteriosclerosis, It is considered to be closely involved in the development of various lifestyle-related diseases such as diabetes. It is said that obesity is caused by constitutional factors, dietary factors, mental factors, central factors, metabolic factors, lack of exercise, etc. As a result, caloric intake exceeds calories burned and fat is accumulated. ing. Lipids in the diet are degraded by pancreatic lipase and absorbed from the small intestine. Therefore, in order to suppress the absorption of lipid, it is considered possible to prevent obesity or suppress hyperlipidemia by using a lipase inhibitor having a function of inhibiting the activity of lipase action.

From this point of view, in particular, a variety of ingredients derived from natural products that can be taken on a daily basis and having a strong lipase inhibitory activity, that is, a natural ingredient having a lipid absorption suppressing activity, is vigorously searched. ing. To date, phosphatidylcholine (K. Taniguti et al., Bull. Facul. Agric. M
eiji Univ. 73, 9-26 (1986), Soy protein (K.
Satouchi et al., Agric. Biol. Chem. 38, 97-101 (19
1974); K. Satouchi et al., Agric. Biol. Chem. 40, 889
~ 897 (1976)), tannin (S. Ahimura et al., Nissho Kogaku, 41, 561-564 (1994)), peony, oren, plantain, button pi, gennoshokou, cha,
Solvent extract of crude drug such as kujin (Japanese Patent Laid-Open No. 64-9013)
1), water extract of bell pepper, pumpkin, shimeji mushroom, maitake mushroom, fuji mushroom, green tea, black tea and oolong tea (JP-A-3-21)
9872), Dokatsu, Ryokyo, Betel nut, Piscine max, Katsumeishi extract (JP-A-5-255100) and the like have been reported as having lipase inhibitory activity, but they are still insufficient in terms of properties and effects of the substance itself. Absent. further,
In such a search, a substance having a large content in the raw material and suitable for industrial production is expected, but a substance sufficiently satisfying such a condition has not been found so far.

In addition, bacterial lipase in the human body is
Microorganisms that are resident on the skin surface (Propionibacterium
Acnes: Propionibacterium acnes, Pitirosporum ovale: Pityrosporum ovale, Micrococcus sp., Etc.), and lipases produced by these lipases decompose triglyceride contained in sebum to produce free fatty acids. Free fatty acids cause an irritating inflammatory reaction to the skin, and are considered to be one of the causes of skin diseases such as acne, dermatitis and dandruff. In particular, there is a correlation between the number of Propionibacterium acnes bacteria, which is the cause of acne, and the amount of free fatty acids produced, and there is a stimulatory inflammatory reaction to the hair follicle wall and the associated hyperkeratosis and comedones. It is believed to cause formation (McGinley, K, J. et al., J. Clin. Microbiol. 12
Vol., 672-675 (1980)).

However, the development of drugs that inhibit or prevent diseases by inhibiting bacterial lipase has not been advanced so far, and 2-Pyridylmethyl-2- (P- (2-methylpropyl) -phen
yl) propinate (common name: ibuprofen piconol)
(Seijin skin, Vol. 47, No. 5, 888-898, 899-908 (1985
Year), tetracycline and metal salts (JP-A-59-18691)
9) and loquat leaf extract as plant extract (JP-A-10-26536)
4) and Cola de Cavallo extract (Japanese Patent Laid-Open No. 11-228338) have been reported, but due to their relationship with other ingredients, they cannot exert lipase inhibitory action, and are safe and effective for topical application. Is not always satisfactory.

[0006]

The object of the present invention is to prevent obesity or suppress hyperlipidemia by using a lipase inhibitor having a function of inhibiting the activity of lipase, or Propionibacterium. An effective and highly safe lipase that is effective in preventing or treating acne and other skin diseases by inhibiting lipase produced by skin-resident bacteria such as Acnes, and has no risk of side effects even after continuous use. It is intended to provide an inhibitor, an anti-obesity agent, a hyperlipidemia-improving agent, a skin-improving agent for acne, and foods, pharmaceuticals and cosmetics containing them.

[0007]

The present inventor has found a strong lipase inhibitory activity in a specific plant and royal jelly, and completed the present invention.

That is, the present invention is directed to Arctos
taphylos uva-ursi), Western primrose (Primula veri)
s), Bodaiju (Ficus religiosa), Asparagus (Asparagus officinalis), Ginkgo bilob
a), Huanarpomacho (Jatropha macrantha), Solidago gigantea, Linden (Tilia
europaea), Katazuba (Erythroxylum vaccinifoliu
m), Eucalyptus (Eucalyptus globulus), Kouki (Enge)
lhardtia chrysolepis), cascara sagrada (Rhamnus
Purshiana), Ononis spinosa, Muyuju (Saraca indica), Polygala senega (Polygala senega) plants, and lipase inhibitors containing at least one selected from the group consisting of royal jelly and lipid absorption containing them. The present invention relates to an inhibitor, an antiobesity agent, a hyperlipidemia improving agent, a skin improving agent for acne, and an oral composition and an external skin composition containing them.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The plants used in the present invention are: Arctostaphylos uva-ursi, Primula veris, Bodaiju (Ficus religiosa),
Asparagus (Asparagus officinalis), Ginkgo (G
inkgo biloba), Huanaru Pomacho (Jatropha macrant)
ha), Solidago gigantea, Linden (Tilia europaea), Katazuba (Erythroxylum v)
accinifolium), Eucalyptus (Eucalyptus globulus),
Kouki (Engelhardtia chrysolepis), Cascara Sagrada (Rhamnus purshiana), Ononis (Ononis spinos)
a), Muyuju (Saraca indica), Senega (Polygala)
senega) is also available, and Royal Jelly (Roy
al jelly) can also be used. These plants and royal jellies may be those commonly used for food.

[0010] Arctostaphylos uva-ursi
Is an evergreen shrub of the dicotyledonous plant of the Ericaceae family, also known as bearberry. It is distributed in highlands in cold regions of Europe, Asia, and North America, and has been growing in rocks, forests, and dry grasslands. Since ancient times, it has been used as a urinary tract disinfectant, astringent, diuretic, urethritis, cystitis, nephritis, nephritis, etc. It has been brewed in.

Western primula (Primula veris) is a perennial plant of the dicotyledonous plant of the Primulaceae family, and is also referred to as Kibananokurinzakura. Native to Europe, Southwest Asia and northern Africa, it is often cultivated in flower beds. In the West, rhizomes, flowers and leaves are bronchitis as an expectorant, pesticide, weak diuretic, laxative and as a syrup,
It is used for coughing. In Japan, it is said that it is effective for people to cut and swell the juice that contains herbal medicine.

[0012] Bodaiju (Ficus religiosa) is a dicotyledonous plant of the Moraceae family, and is also called Indian Bodaiju. It is an evergreen large tree distributed in India and Ceylon. It has broad-ovate heart-shaped leaves and a pointed tip. The Bodhi tree, which is said to have been enlightened by the Buddha under the tree, is the Indian bodige.

Asparagus (Asparagus officinalis)
Is a monocotyledonous plant of the family Liliaceae, also known as the matsubaud or the pheasant beetle, and in China it is known as Ishigashi. Native to Southern Europe, it came to Japan in 1781, and was originally for appreciation, but is now a perennial plant that is cultivated in Hokkaido, the Tohoku region, and Nagano prefecture for food. In China, stone cough is said to have antitussive, expectorant, and anthelmintic effects, and is used for lung fever, worm insects, scabies and the like. In addition, it is also reported that the peripheral blood vessels are expanded by intravenous injection to enhance the contractile function of the heart to improve blood pressure, and the diuretic effect to increase urine volume.

[0014] Ginkgo biloba is a tall tree, a gymnosperm plant of the ginkgo genus. It is a deciduous tree native to China and widely planted all over Japan. It has been used for antitussive and nocturnal enuresis since ancient times. In recent years, an effect of improving blood flow has been reported, and it is also used as a health supplement for the purpose of improving blood flow and improving brain function.

Huanaru Pomacho (Jatropha macrantha)
Is a dicotyledonous plant of the Euphorbiaceae family, which grows naturally on the Andean plateau, and in Peru, stem extracts have been used as aphrodisiac agents since the Inca Empire.

[0016] Solidago gigantea
Is a dicotyledonous plant of the Asteraceae family and is also called as another name. It is a perennial plant that is distributed from Hokkaido to Kyushu and the Korean Peninsula and grows in the mountainous and flat Muyang lands. Decoction is effective for the treatment of experimentally generated bronchitis and also has an expectorant effect. Suppressive against Staphylococcus aureus, Streptococcus pneumoniae, Bacillus aureus, and Shigella. Further, clinically, the preparation is effective for treating respiratory tract infection, tonsillitis, sore throat, bronchitis, etc. It is also used as an antiphlogistic, detoxification, and analgesic for headache during colds, sore throat, jaundice, whooping cough, and the like.

Linden (Tilia europaea) is a dicotyledonous plant of the lindenaceae, and is also referred to as Boletus edulis or lime tree. Flowers have the effect of calming the nerves and are used as tea for sleep, colds, weak stomach, and recovery from fatigue. Also, xylem has an excellent diuretic effect,
Since it promotes the discharge of waste products, it is used to prevent kidney stones.

Caturba (Erythroxylum vaccinifoliu
m) is a dicotyledonous plant of the family Coccinaceae, which is also called tutufassa, tatsuaba, or Paul reposta. It is a plant that grows in the southern part of the Amazon river basin, etc. in Brazil, and it has long been known that it has an aphrodisiac effect,
Used in folklore. Further, in recent years, it has been reported that it has a central nervous system stimulating action and a tonic action, and it is also used for male sexual function recovery (erection force).

Eucalyptus globulus is a dicotyledonous plant of the family Myrtaceae, native to Australia,
It is an evergreen tree that is cultivated all over the world. The leaves show antibacterial action against many bacteria, and also have anthelmintic action and expectorant action. Eucalyptus leaves are used for colds, diarrhea, arthralgia, enteritis, burns, candies, eczema, neurodermatitis, etc.

Kouki (Engelhardtia chrysolepis) is
It is a dicotyledonous plant of the walnut family, and is an evergreen tree that grows naturally in the mountains of Guangdong Province, Guangxi Province in southern China. Since it has a weak sweetness, it has been used as sweet tea for a long time, and it is said to have antipyretic, deficient, analgesic, stomachic and slimming effects.

Cascara Sakurada (Rhamnus purshiana)
Is a dicotyledonous plant of the buckthorn family and is a deciduous shrub that is native to western North America such as Montana and California and is partly cultivated. A small amount of bark is used as a bitter stomach medicine, and an increased amount is used as a laxative.

Ononis spinosa is a dicotyledonous plant of the leguminous family, which is a semi-shrub that is distributed in Asia and Europe and is found in dry grasslands, roadsides, rims of fields and the like. Roots, leaves and flowers are used for diuresis and stone dissolving agents.

Muca (Saraca indica) is a dicotyledon plant of the legume family and belongs to the subfamily Coccinaceae. Also known as Ashokanoki and Ashokaju, it is a tall tree distributed from India to Southeast Asia. The material is hard,
It is used as a building material and furniture material, and is sometimes used as a roadside tree in the tropical zone.

[0024] Senega (Polygala senega) is a dicotyledonous plant of the family Scutellariae, and is called Bienshi in China. It is a perennial shrub that grows in the mountains of eastern North America. It is said that the roots contain saponin glycosides, and its mucosal stimulatory action reflexively stimulates airway secretion and thins viscous sputum. Therefore, it is used as an expectorant and antitussive drug.

Royal jelly is secreted by young worker bees. Pollen and honey eaten by worker bees are digested and absorbed in the intestines to become nutrients.
Furthermore, it is transported to the pharyngeal glands in the head through the spinal column and stored as royal jelly. The worker bees are secreted from the mouth and throat when necessary, and are fed as food for the queen bees and as food for the queen bee larvae. Royal jelly is an open glandular secretory, sour milky milky emulsion, which is why it is also called "royal milk." In recent years, it has been used as a health food with a rejuvenating effect, which is called a “drug for longevity and longevity”.

The plants in the present invention include leaves, stems, buds, flowers,
All parts such as above-ground parts such as wood and bark (bark) and roots, below-ground parts such as tubers, seeds, fruits and resins can be used.

The plant in the present invention is a dried product obtained by drying the plant itself, a crushed product thereof, squeezed juice obtained by squeezing and extracting them, water, or a crude extract with an organic solvent such as alcohol, ether or acetone. , And an extract fraction obtained by purifying the crude extract stepwise by various chromatographies such as partition chromatography and the like. These may be used alone or 2
You may use it in mixture of 2 or more types.

For example, Asparagus offici
3 liters of 99.5% ethanol extract was added to 1 kg of dried plant matter of Nalis), and the extract obtained by soaking overnight at room temperature was used as it was, lipase inhibitor and lipid absorption inhibitor containing them, antiobesity agent, It may be used as a hyperlipidemia-improving agent or a skin-improving agent for acne, or may be purified by combining various chromatographies.

It is a novel finding obtained by the present invention that the dried or extract of the specific plant according to the present invention and the royal jelly according to the present invention have no lipase inhibitory activity.

The plants and royal jelly according to the invention are
It has an excellent lipase inhibitory activity, and can be used as an antiobesity agent, a hyperlipidemia improving agent, a skin improving agent for acne, an oral composition containing the same, and an external skin composition.

The plant and royal jelly of the present invention can be produced as a lipase inhibitor, an antiobesity agent, a hyperlipidemia improving agent, a skin improving agent for acne, and foods, pharmaceuticals and cosmetics containing them.

The lipase inhibitor, the antiobesity agent, the hyperlipidemia improving agent and the medicine containing them of the present invention can be used either orally or parenterally. Upon administration, the active ingredient is orally, transdermally, rectally,
It can be administered in the form of a conventional pharmaceutical preparation by mixing with a solid or liquid pharmaceutical non-toxic carrier suitable for administration methods such as injection. Such formulations include, for example, tablets,
Examples include solid agents such as granules, powders and capsules, solutions such as solutions, suspensions and emulsions, freeze-dried preparations, ointments and suppositories. These preparations are prepared by a conventional formulation method. be able to. Examples of the non-toxic carrier for pharmaceutical use include glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glyceride, polyethylene glycol, hydroxyethyl starch, ethylene glycol, polyoxyethylene sorbitan fatty acid ester, amino acid, gelatin, Albumin, water, physiological saline and the like can be mentioned. Further, if necessary, conventional additives such as a stabilizer, a wetting agent, an emulsifier, a binder and an isotonicity agent can be appropriately added.

As a food, it is eaten as a food for health use or a food material useful for the treatment and prevention of hyperlipidemia or obesity, as it is, or by adding various nutritional components or by including it in foods and drinks. . For example, after adding the above-mentioned suitable auxiliaries, using a conventional means, a form suitable for food, such as granules, granules, tablets, capsules, paste, etc. may be provided for food, Also various foods,
For example, processed meat products such as ham and sausage, processed fish products such as kamaboko and chikuwa, bread, confectionery, butter,
It may be used by adding it to milk powder or fermented dairy products, or by adding it to beverages such as water, fruit juice, milk and soft drinks.

The effective dose of the plant of the present invention is appropriately selected and determined according to the age, body weight, symptom of the patient, degree of the patient, administration route, administration schedule, formulation form, strength of inhibitory activity of the material and the like. However, for example, in the case of oral administration, it is generally about 0.001 to 1000 mg / kg body weight per day, and in the case of a hot water extract of huanarpomacho (Jatropha macrantha), 1
The dose may be about 5 to 300 mg / kg body weight per day, and may be administered in several divided doses per day.

When the plant of the present invention is used and used as a lipase inhibitor-containing cosmetic material or cosmetic material, for example, a Japanese lacquer tree (Arctostaphylos uva-urs) is used.
The dried product or extract of i) is added to wheat germ oil or olive oil to give a lipase inhibitor-containing composition, which can be used as a cosmetic material. The addition amount of the dried product or extract of the plant of the present invention is not particularly limited, but as an example, 0.1% by weight or more and 60% by weight or less with respect to the weight of wheat germ oil or olive oil, preferably 0.5 wt% or more and 50 wt% or less is suitable.

Further, the above lipase inhibitor-containing composition can be directly used as a cosmetic ingredient to produce a cosmetic having a lipase inhibitory action. Cosmetics include oils and fats such as vegetable oils, higher fatty acids, higher alcohols, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, preservatives, sugars, sequestering agents. , A polymer such as a water-soluble polymer, a thickener, a powder component, an ultraviolet absorber, an ultraviolet blocking agent, a moisturizer such as hyaluronic acid, a fragrance, a pH adjusting agent, a desiccant, etc. can be contained. . Other medicinal and physiologically active ingredients such as vitamins, skin activating agents, blood circulation promoting agents, indigenous bacteria control agents, active oxygen scavengers, anti-inflammatory agents, whitening agents and bactericides may also be contained.

The cosmetics include skin lotions such as lotions, emulsions, creams and packs, makeup base lotions, makeup creams, emulsions or creams or ointment type foundations, lipsticks, eye colors,
It can be used as makeup cosmetics such as blush color, body creams such as hand creams, leg creams and body lotions, bath salts, oral cosmetics and hair cosmetics. The cosmetic containing the lipase inhibitor-containing composition obtained by the method of the present invention is preferably, for example, an emulsion, a cosmetic, a face cream, a hand cream, a lotion, an essence, a shampoo, or a rinse from the viewpoint of function.

Such cosmetics can be produced according to a conventional method. The amount of the dried product or extract of the plant of the present invention in the cosmetic is not particularly limited, but as an example, 0.01% by weight or more and 20% by weight or less of the total weight of the cosmetic is suitable. .

Since the plant of the present invention is a natural product, its toxicity is low. For example, a hot water extract of Muyuju (Saraca indica) is orally administered to rats at a daily dose of 1000 mg / kg for 100 days. However, no deaths were observed and no change in body weight was observed.

[0040]

EXAMPLES The present invention will be specifically described below with reference to examples, but the invention is not limited thereto.

Production Example [Extraction of plant] Arctostaphylos uva-urs
i), Western primrose (Primula veris), Bodaiju (Ficus religiosa), Asparagus (Asparagus offic)
inalis), Ginkgo biloba, Huanarpomacho (Jatropha macrantha), Solid (Soli)
dago gigantea), Linden (Tilia europaea), Katazuba (Erythroxylum vaccinifolium), Eucalyptus (Eu)
calyptus globulus), Japanese fir (Engelhardtia chrysole
pis), Cascara Sagrada (Rhamnus purshiana), Ononis (Ononis spinosa), Muyuju (Saraca indic)
a), Polygala senega plant and Royal jelly 10Kg each, 3L distilled water was added,
℃, 1500P.SI for 20 minutes, after boiling, by freeze-drying, each hot water extract dried product 2541, 1965, 2002, 1789,
1452, 986, 1678, 2170, 3123, 2546, 3256, 986, 326
5, 2147, 3269 and 2143 mg were obtained.

Pharmacological test [Lipase inhibitory activity test] {Preparation of substrate (10% soybean oil / gum arabic emulsion)} 1 g of gum arabic is dissolved in about 5 ml of distilled water and dialyzed against 5 L of distilled water overnight. Then, put gum arabic from a dialysis tube into a 20 ml meter glass and make 20 ml while co-washing. Also, weigh 1 g of soybean oil into a 50 ml beaker. To this, add 10 ml of the above 5% gum arabic,
Stir well with a stirrer. {Preparation of copper mixed solution} 6.45% Copper (II) nitrate trihydrate 100m
90 ml of 1M triethanolamine and 10 ml of 1N acetic acid were added to prepare 200 ml of a copper mixed solution. {Preparation of coloring reagent (0.1% DEDC)} 10 mg of sodium N, N-diethyldithiocarbamate trihydrate was dissolved in 10 ml of butanol. {Inhibitory activity test} K. Satouc
Hi et al. (Agric. Biol. Chem. 40, 889-897 (1
976)) and measured by the Duncombe method.

That is, 0.5M Tris-HCl (pH7.4) buffer solution
50μl, 50μl of 10% soybean oil emulsion as a substrate, 5m
M 100 μl of calcium acetate and 100 μl of an aqueous solution of the dried plant extract (final concentration: 500 μg / ml) obtained in the Production Example are added, and then distilled water is added to make 450 μl. This is 37 ° C, 65r.p.
After pre-incubation for 5 minutes at m., 50 μl of lipase (manufactured by Sigma, derived from bovine pancreas) (1 mg / ml) was added, and the mixture was incubated at 37 ° C. and 125 rpm for 20 minutes. Then add 3.5 ml of chloroform and remove the upper layer,
To the remaining lower layer, 1.5 ml of a copper mixed solution is added, and the mixture is stirred with a concentrator for 15 minutes, and then the upper layer is removed. After adding 0.5 ml of coloring reagent (0.1% DEDC) to the remaining lower layer, the lipase inhibitory activity was measured by measuring the absorbance of the free fatty acid at 440 nm, and the final concentration of 500 μg / ml was used as a control ( Table 1 shows the results of the inhibition rate (%) with respect to the one obtained by adding distilled water instead of the dried plant extract.

[0044]

[Table 1]

In addition, as a comparative example, Ketsumeishi, Kola de J., which has already been reported to have lipase inhibitory activity,
Similar tests were conducted on Kavalo. The results are also shown in Table 1.

As can be seen from Table 1, Arctostaphylos uva-ursi of the present invention, Western primrose (P)
rimula veris), Bodaiju (Ficus religiosa), Asparagus (Asparagus officinalis), Ginkgo (Gin)
kgo biloba), Huanaru Pomacho (Jatropha macranth)
a), Solidago gigantea, Linden (Tilia europaea), Erythroxylum vac
cinifolium), eucalyptus (Eucalyptus globulus), kouki (Engelhardtia chrysolepis), cascara sagrada (Rhamnus purshiana), ononis (Ononis spinos)
a), Muyuju (Saraca indica), Senega (Polygala)
It can be seen that the extract of senega plant and royal jelly has high lipase inhibitory activity.

Formulation Example 1 [Production of tablets] Asparagus (Asparagu) obtained in the production example
s officinalis) using hot water extraction according to a conventional method,
A tablet having the following composition was produced. (Composition) (Composition: wt%) Asparagus extract dried product 24 Lactose 63 Corn starch 12 Guar gum 1

Formulation Example 2 [Production of juice] Mujuju (Saraca) obtained in the production example
Indica) hot water extract was used to produce a juice having the following composition according to a conventional method. (Composition) (Composition: wt%) Frozen concentrated Unshu mandarin orange juice 5.0 5.0 Fructose glucose liquid sugar 11.0 Citric acid 0.2 L-Ascorbic acid 0.02 Perfume 0.2 Pigment 0.1 Mujuju extract dried product 2 Water 83.28

Formulation Example 3 [Production of face cream] Using the hot water extract of royal jelly obtained in the production example, a face cream having the following composition was produced according to a conventional method. (Composition) (Formulation: wt%) Isopropyl isostearate 8.0 Jojoba oil 6.0 Cetanol 8.0 Stearyl alcohol 2.0 Polyoxyethylene lauryl ether 1.5 Propylene glycol 6.0 Sorbitol 1.0 Paraben 0.4 Royal jelly extract dried product 0.5 Vitamin E 0.5 Perfume 0.1 Purified water 66.0

[0050]

The plant and royal jelly of the present invention strongly inhibit the lipase activity, and thus can contribute to the suppression and prevention of obesity and hyperlipidemia, as well as acne and dermatitis caused by bacterial lipase, It is effective in preventing and treating skin diseases such as dandruff.

─────────────────────────────────────────────────── ─── Continued Front Page (51) Int.Cl. ⁷ Identification Code FI Theme Coat (Reference) A61K 35/78 A61K 35/78 L S T 35/64 35/64 A61P 3/04 A61P 3/04 3 / 06 3/06 43/00 111 43/00 111 C12N 9/99 C12N 9/99 // A61K 7/00 A61K 7/00 K 7/48 7/48 F term (reference) 4C083 AA071 AA072 AA111 AA122 AC072 AC122 AC132 AC182 AC352 AC482 AD662 CC02 CC05 DD31 EE14 EE23 4C087 AA01 AA02 BB22 MA02 MA52 MA63 NA14 ZA70 ZA89 ZC20 ZC33 4C088 AB02 AB11 AB26 AB34 AB44 AB46 AB59 AB85 AC03 AC04 AC05 AC06 AC08 AC11 AC12 AC13 BA20 C02 MA33 NA89 ZA33

Claims (9)

[Claims] 1. At least one selected from the group consisting of oak, primrose, western primrose, bodaiju, asparagus, ginkgo, huanarpomacho, velvetflower, linden, catazuba, eucalyptus, kouki, cascara sagrada, ononis, muyuju, senega plant and royal jelly. A lipase inhibitor containing a seed. 2. A lipid absorption inhibitor containing at least one plant or royal jelly according to claim 1. 3. An acne preparation containing at least one plant or royal jelly according to claim 1. 4. An anti-obesity agent containing at least one species of Japanese lacquer tree, western primrose, primrose, bodai, asparagus, ginkgo, huanarpomacho, oleander, linden, katava, cascara sagrada, ononis, muyuju, senega plant or royal jelly. 5. An antiobesity agent containing eucalyptus or kouki as a lipase inhibitor. 6. Hyperlipidemia containing at least one plant of crocodile, western primrose, bodice, asparagus, huanarpomacho, oleracea, linden, katava, eucalyptus, kouki, cascara sagrada, ononis, muyuju, senega, or royal jelly. Improver. 7. Ginkgo biloba as a lipase inhibitor,
Agent for improving hyperlipidemia. 8. An oral composition containing any one of claims 1 to 7. 9. A composition for external use for skin, containing the composition according to claim 1.