KR101910013B1 - A composition for improving, preventing and treating of pain comprising herb extract - Google Patents

Abstract

The present invention relates to a composition for improving, preventing and treating pain, which comprises a mixed extract composed of Cyperus rotundus, Lindera aggregate, Inula helenium, Glycyrrhiza uralensis, Platycodon grandiflorum and Citrus aurantium as an active ingredient, and thus pain caused by the administration of reserpine can be ameliorated, prevented or treated. In addition, since the composition is not toxic, the composition can be ingested in the form of food.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for improving, preventing, or treating pain comprising a herbal medicine extract (SOCG)

The present invention relates to a composition for improving, preventing or treating pain comprising as an active ingredient a mixed extract (referred to as SOCG) consisting of a perfume, a fragrance, a moss, a licorice, a longitude and a crust.

There are various categories of pain, but they are divided into acute pain, which acts as a biological warning system depending on the period or nature, and chronic pain, which continues to be painful even though the period of time required for normal healing is exceeded.

Depending on the cause, there are three broad categories: invasive pain, neuropathic pain, and psychogenic pain. The fibromyalgia is a chronic pain disorder with various symptoms including insomnia, general fatigue, and the like, which are the main symptom of chronic pain that is difficult to tolerate by the whole body. Symptoms of fibromyalgia are very diverse.

Symptoms of fibrogenic pain include chronic pain of deep tissues such as muscle tissue and pain accompanied by acupressure. In addition, there are many cases of allodynia such as contact allodynia and cold allodynia, and thermal hyperalgesia.

In pain disorders, the cause of pain is not clear, while the physical or functional impairment of the cause of pain is somewhat clear. The criteria for the diagnosis of fibromyalgia are defined by the American College of Rheumatology as a result of extensive physical pain of the body lasting more than 3 months and the presence of systemic (ligaments, tendons, muscles, etc.) And the tenderness appears at eleven or more of the tender points of the place.

This diagnostic criterion is clearly different from the diagnostic criteria for other pain disorders. In other words, fibromyalgia is an independent chronic disease that is distinct from other pain disorders in terms of symptoms, causes of pain, and criteria for diagnosis.

Recently, drugs such as pregabalin, duloxetine, a SNRI (serotonin nor noradrenaline re-uptake inhibitor) and pramipexole, a dopamine agonist, were statistically significant , But the efficacy of these agents is limited.

Even now, safe and effective treatments for fibromyalgia have not yet been established, and there is a strong demand for the development of new excellent therapeutic agents with sufficient effects and fewer side effects.

Korean Patent No. 1318330 Korean Patent No. 1386791

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating pain comprising as an active ingredient, a mixed extract (called SOCG) consisting of root extract, fragrance, sorghum, licorice,

Another object of the present invention is to provide a food composition for preventing or ameliorating pain comprising as an active ingredient, a mixed extract (called SOCG) consisting of a herbal extract, a fragrant extract, a licorice extract, a licorice extract,

In order to accomplish the above object, the pharmaceutical composition for preventing or treating pain according to the present invention may contain, as an active ingredient, a mixed extract comprising a herbal composition, a fragrance, a herb, a licorice, a ginseng, and a crust.

The perfume, the scent, the seedlings, the licorice root, the root length, and the crust are mixed in a weight ratio of 1: 0.2-0.8: 0.05-0.3: 0.05-0.3: 0.2-0.8: 0.2-0.8, : 0.05-0.15: 0.05-0.15: 0.4-0.6: 0.4-0.6.

The mixed extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof.

The pain may be pain caused by the administration of reserpine.

The pain may be fibromyalgia.

In another aspect of the present invention, there is provided a food composition for preventing or improving pain comprising as an active ingredient, a mixed extract consisting of a herbal supplement, a fragrance, a herb, a licorice, a ginseng, and a crust.

The composition capable of improving, preventing or treating the pain comprising the mixed extract of the present invention as an active ingredient is not toxic and has an analgesic effect on the pain caused by the reserpine It is highly effective in improving, preventing or treating pain and is therefore effective for manufacturing competitive food compositions and pharmaceutical compositions.

FIG. 1 is a photograph showing a process for measuring physical allodynia.

The present invention relates to a composition for improving, preventing or treating pain comprising as an active ingredient a mixed extract (referred to as SOCG) consisting of a perfume, a fragrance, a moss, a licorice, a longitude and a crust.

The pain is pain caused by the administration of reserpine, specifically fibrosis pain.

Hereinafter, the present invention will be described in detail.

The composition for improving, preventing or treating the pain of the present invention contains as an active ingredient a mixed extract consisting of a herbal extract, a fragrance, a moss, a licorice, a longitude and a crust.

Cyperus rotundu has red flowers in July to August, two rows of flowers, and fruit ripens in October. In the oriental medicine, it is used for the penetration of tuberous root, analgesic, cold, landscape, dryness, tornado, hari, wrinkle, neuralgia, pulmonary tuberculosis and women's diseases. Major components are essential oil Cyperene and Cyperol. Medicinal area is rhizome. Is a medicinal herb that has been said to be a gynecological pre-disposition. It has a special effect on menstrual irregularities, prenatal postpartum headache, menstruation, blood circulation, nervous stability, strengthening of strength, weakness of chronic gastric dysfunction, .

Further, the above-mentioned smell ( Lindera aggregate; Lindera syrychnifolia ) is a camphoraceae plant, with oval or egg-shaped leaves staggered, and has a yellow flower. It grows in southern China (Fujian, Zhejiang, Jiangsu, Taiwan) and has 0.1 ~ 0.2% essential oil in roots. The essential oils include terenine compounds such as lindaneol (lindaneol (lindane), lindestrene, linderolactone, etc.), and mainly dried roots are used for medicinal purposes. It is said that the stomach is used for the hyperactivity of the brain, the pericardial paralysis, the headache, and the circulation of the body (气), and the stomach Warming, polyuria, diarrhea, diarrhea, and use in the month.

In addition, the Aucklandia lappa Dene (Aucklandia lappa Dene) is obtained by cooling essential oils (1-5%) in roots and rootstocks, which are called hellenin. And inulin, pseudo-inulin, saponin, bitter substances, and so on. Others include scopoline and umbeliferone. It is good for chest pain, dysentery, vomiting, and the root lunar drip is strong in excretion of phlegm and inhibits anti-inflammatory action, hypertrophic intestinal motility and secretion. Essential oil has side effects and anti-inflammatory action, and it is used for various respiratory diseases such as phlegm, placebo, diuretic, herbal medicine, bronchitis and pulmonary tuberculosis.

The licorice (Glycyrrhiza uralensis FISCH.) Is a perennial herb of soybean, and licorice is harmonized with all the medicines in herbal medicine and not only increases the efficacy, but also alleviates various pain and urgency symptoms through relaxation and analgesic medication. It is used for external use, and itself has a weakness such as a neutralizing action of the poison. For example, licorice has a medicinal effect to control fatigue by protecting the liver and facilitating liver function, and when the licorice is taken, the burden of liver is reduced by its detoxifying action. It has all the detoxification action against bacterial toxins as well as drug poisoning, food poisoning, metabolism, poisoning by containing triterpene saponin and glycyrrhizin in root and root of plant, Action and regulating the amount of thyroid sebum hormone. Licorice exhibits anti-inflammatory and anti-allergic effects, acts antagonistic to acetylcholine, and has the effect of enhancing the adrenaline action of adrenaline

In addition, Gil Kyung is a root of Platycodon grandiflorum A. DC, which is a plant of Campanulaceae. It has been mainly used for the treatment of geomorphic, sea bream, cough and bronchitis. Through NF-kB and NO essay Anti-inflammatory effect, anti-cancer effect, pain relieving effect, suppression of digestive enzyme secretion, and improvement of cholesterol metabolism.

In addition, the crust is dried to the immature fruit of citrus aurantum. (Rice and Rutacceae), and the taste is cold and nasty, and the taste is spicy. The crust increases the excitability of gastrointestinal and uterine smooth muscle, and also regulates the gastrointestinal interactions.

The weight ratio of the fragrance, the fragrance, the horseradish, the licorice, the root length and the crust is 1: 0.2-0.8: 0.05-0.3: 0.05-0.3: 0.2-0.8: 0.2-0.8, preferably 1: 0.4-0.6: 0.06-0.2: Preferably in a weight ratio of 0.06-0.2: 0.4-0.6: 0.4-0.6, more preferably in a weight ratio of 1: 0.45-0.55: 0.1-0.15: 0.1-0.15: 0.45-0.55: 0.45-0.55.

In the case where the contents of omega, sorghum, licorice, licorice, perianth and crust are less than the lower limit based on the herbal supplement, the effect of improving, preventing or treating pain may be lower than that of each medicinal herb alone, The effect of improving, preventing or treating pain may be reduced.

In case of using only the herbal supplement, omega, sorghum, licorice root, gill root, and crust, the efficacy is 1.5 to 20 times lower than that of herbal supplement, omega, sorghum, licorice root,

The mixture of the herbal extract, the ocher, the moss, the licorice, the gill, and the crust is mixed with the extraction solvent at a weight ratio of 1: 5 to 25, preferably 1: 8 to 15, and extracted at 90 to 110 to prepare an extract. When the weight ratio of the mixture of the herbal supplement, the ocher, the mixture of liquorice, liquorice, ginseng, and crust is out of the above range, the active ingredient of the mixture of the herbal supplement, the oyster, the liquorice, the licorice, have.

The extraction solvent for extracting each of the above extracts is water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. The extraction solvent is not particularly limited, but an extract extracted with water is preferably used for improving, preventing or treating pain.

In this specification, the term extract used in reference to a mixture of fragrance, fragrance, vinegar, licorice, licorice, persimmon and crust is not limited to crude extract obtained by treating an extracting solvent, . For example, mixed extracts of herbal extracts, fragrances, sorghum, licorice, longgrass and crust can be prepared in powder form by additional processes such as vacuum distillation and freeze drying or spray drying.

In addition, the mixed extract of fragrant, ocher, sorghum, licorice, longitude, and crust of the present invention can be broadly divided into fragrant fractions, fragrance fragrances, fragrance fragrances, Gypsum, licorice, longitude, and crust, such as a mixed powder of a herbal extract, a mist, a vinegar, a licorice, a long grain, and a crust. Although the present invention has been carried out with a mixture of a perfume, a fragrance, a vinegar, a licorice, a licorice, and a crust, the desired effect can be achieved in the form of a mixture of fragrance, fragrance, Can be expected by one skilled in the art.

As used herein, the term " comprising as an active ingredient " means an amount sufficient to achieve the efficacy or activity of the herbal extract, frail, moss, licorice, For example, the combined extracts of the herbal extract, the scent, the moss, the licorice, the root and the crust are used at a concentration of 10 to 1500 μg / ml, preferably 100 to 1000 μg / ml. Since the mixed extract of the herbal supplement, the miso, the licorice, the licorice, the gill, and the crust is a natural product, there is no adverse effect on the human body even when administered in an excessive amount. Therefore, the quantitative upper limit of the mixed extract of the herbal supplement, May be selected and carried out by a person skilled in the art within a suitable range.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile solutions suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention can be administered orally or parenterally. In the case of parenteral administration, the composition can be administered by intravenous injection, subcutaneous injection, muscle injection, intraperitoneal injection, transdermal administration, etc., .

The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Usually, a skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g / kg.

The pharmaceutical composition of the present invention can be prepared in unit dose form by formulating it with a pharmaceutically acceptable carrier and / or excipient or can be manufactured by inserting it into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

In addition, the present invention provides a food composition for improving, preventing or treating pain comprising as an active ingredient, a mixed extract of a herbal supplement, a fragrance, a moss, a licorice, a ginseng, and a crust.

The food composition according to the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectioneries, diet bars, dairy products, meat, chocolates, pizza, ram noodles, other noodles, gums, ice cream, .

The food composition of the present invention may contain, as an active ingredient, components that are commonly added in the manufacture of food, as well as mixed extracts of herbal ingredients, miscellaneous ingredients, herbs, licorice, , Nutrients, flavoring agents, and flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, it may contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, Various plant extracts, and the like.

The present invention provides a health functional food comprising the food composition for improving, preventing or treating pain comprising the combination extract of the fragrance, the fragrance, the moss, the licorice, the gill, and the crust as an active ingredient. The health functional food is a food prepared by adding a mixed extract of a herbal supplement, a miso, a sorghum, a licorice, a ginseng, and a crust to a food material such as a beverage, a tea, a spice, a gum and a confection or an encapsulation, a powder, This means that it takes a certain effect on health when consumed, but unlike general medicine, there is an advantage that there is no side effect that may occur when a drug is taken for a long time by using food as a raw material. The health functional food of the present invention thus obtained is very useful because it can be ingested routinely. The amount of the mixed extract of the fragrance, the fragrance, the liquorice, the licorice, the ginseng, and the crust of the health functional food can not be uniformly determined depending on the type of the health functional food as a target, , And it is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight based on the target food. In the case of health functional foods in the form of pills, granules, tablets or capsules, they may be added usually in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides the use of herbal extracts, fragrances, sorghum, licorice, ginseng, and crust extracts for the manufacture of medicines or foods for the improvement, prevention or treatment of pain. As described above, the combined extracts of rosemary, fragrance, moss, licorice, rootstock, and crust can be used for improving, preventing or treating pain.

The present invention also provides a method of improving, preventing or treating pain comprising administering to a mammal an effective amount of a combined extract of rosemary, myrrh, sorghum, licorice, licorice, longitude and crust.

The term "mammal " as used herein refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term "effective amount" refers to the amount of active ingredient or pharmaceutical composition that elicits a biological or medical response in a tissue system, animal, or human, as contemplated by a researcher, veterinarian, physician or other clinician, ≪ / RTI > inducing a reduction of the symptoms of the disease or disorder. The effective amount and the administration frequency for the active ingredient of the present invention can be changed according to the desired effect. Thus, the optimal dosage to be administered can be readily determined by those skilled in the art and will vary with the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, The age, body weight, sex, diet, time of administration, route of administration and fraction of the composition, duration of treatment, concurrent medication, and the like. In the prevention, treatment, or improvement method of the present invention, when an adult is administered once or several times a day with a mixed extract of rosacea, fragrance, sorghum, licorice root, Dose is preferable.

In the treatment method of the present invention, the composition comprising the mixed extract of rosemary, fragrance, sorghum, licorice, gill, and crust as an active ingredient can be administered orally, rectally, intravenously, intraarterially, intraperitoneally, intramuscularly, , Intraocularly or intradermally, in a conventional manner.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Example  One. Herbalist : Broken egg : elecampane :licorice: Gakyung : Crust = 1: 0.5: 0.12: 0.12: 0.5: 0.5

0.5, 0.1: 0.12: 0.5: 0.5, weight ratio of herbal extract, miso, liquorice, licorice, gill, and crust purchased from Han Kook Shin Yak Pharmaceutical Co., Ltd., Nonsan, Then, the mixture was mixed with water at a weight ratio of 1:10, and the mixture was extracted with hot water at 90 for 3 hours to obtain a combined extract of herbal extract, fragrant, moss, licorice, longitude and crust.

Comparative Example 1 Weight ratio of fragrance: ocher: corn liquor: licorice: persimmon: crust = 1: 1.5: 0.5: 0.5:

The mixture was mixed in the same manner as in Example 1 except that the fragrance, the scent, the liquorice, the licorice, the root, and the crust were mixed at a weight ratio of 1: 1.5: 0.5: 0.5: 1.5: 1.5 to obtain a mixed extract.

<Test Example>

Control, Examples and Comparative Examples The extract was filtered, and the filtrate was concentrated under reduced pressure at 60 or less.

Test Example 1. Pain test

250-300 g of Wistar rats (SAMTAKO, Korea, male) were used in the experiment. The animals were housed in an acrylic cage (45 x 60 x 25 cm), fed with sufficient feed and water, and adjusted for 12 hours of daytime and night with appropriate artificial illumination (from 8:00 am). And maintained a constant temperature (20 ~ 24) and humidity (45 ~ 65%). We observed for a week to adjust to the changed environment, maintained the sleep cycle, and confirmed abnormal behavior.

All experiments using animals were performed in compliance with the MFDS's National Institute of Toxicological Research for the care and use of laboratory animals (KHUASP (SE) -14-051) .

Experimental animals were divided into two groups according to which the animals were divided into the normal group in which physiological saline was administered by randomly selecting healthy animals with a constant weight change, the control group in which reserpine was administered, the group in which reserpine was administered, And the sample administered with the extract of Comparative Example 1 were used. Five mice were used per experimental group.

The extracts of Example 1 and Comparative Example 1 were administered at the following concentrations (100, 200, 300 mg / kg) at a dose of 1 mg / kg once a day for 3 days Dissolved in saline and orally administered at a dose of 100 μl.

-8 groups of mice -

Normal group: 3 days of physiological saline 5 mg / kg / day

Control group 1: 3 days of lecerpine 5 mg / kg / day

Control group 2: lecerpine for 3 days _1 mg / kg / day + gabapentin _50 mg / kg (intraperitoneally)

Example 1 (100): Lercerpine for 3 days _1 mg / kg / day + Extract of Example 1 100 mg / kg

Example 1 (200): Lercerpine for 3 days _1 mg / kg / day + Extract of Example 1 200 mg / kg

Example 1 (300): Lercerpine for 3 days _1 mg / kg / day + Extract of Example 1 300 mg / kg

Comparative Example 1 (100): Lercerpine for 3 days _1 mg / kg / day + Extract of Comparative Example 1 100 mg / kg

Comparative Example 1 (200): Lecerpine for 3 days _1 mg / kg / day + Extract of Comparative Example 1 200 mg / kg

Comparative Example 1 (300): Lecerpine for 3 days _1 mg / kg / day + Extract of Comparative Example 1 300 mg / kg

Analgesic effect measurement

The physical allodynia test was performed 30 minutes after the oral administration, and the physical allodynia was a painful response when a weak stimulus was applied to cause a simple tactile response without causing pain in a healthy state (Fig. 1) . The animals were placed in a cage with a wire floor for the measurement of mechanical allodynia. The withdrawal threshold was measured in the ipsilateral and / or contralateral hindpaw by von Frey filament (Somedic AB, Malmo, Sweden). The median of the five stimuli provided data points. The half-maximal effective dose (ED50) with a corresponding 95% confidence limit was determined by semi-log regression analysis at maximum activity.

The area under the curve (MA area under the curve) indicates the degree of pain relief.

division Normal group Control 1 Control group 2 Example 1 (100) Example 1 (200) Example 1 (300) Comparative Example 1 (100) Comparative Example 1 (200) Comparative Example 1 (300) area
(A measure of pain) 38 342 202 217 201 210 336 330 325

As shown in Table 1 above, the extract prepared according to Example 1 of the present invention showed similar analgesic effects as the control 2 administered with the analgesic agent. In particular, the group administered with the extract of Example 1 at 200 mg / kg exhibited a similar analgesic effect to that of the control group 2 as compared with the other groups.

On the other hand, it was confirmed that the extract of Comparative Example 1 had less analgesic effect than the extract of Example 1.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  One. Sanje  Produce

500 mg of the extract powder obtained in Example 1

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation example  2. Preparation of tablets

300 mg of the extract powder obtained in Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

200 mg of the extract powder obtained in Example 1

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

600 mg of the extract powder obtained in Example 1

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

It is prepared by the above-mentioned component content per ampoule according to the usual injection preparation method.

Formulation example  5. Liquid  Produce

4 g of the extract powder obtained in Example 1

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 g with purified water, To prepare a liquid agent.

Formulation example  6. Preparation of granules

1,000 mg of the extract powder obtained in Example 1

Vitamin mixture quantity

Vitamin A Acetate 70

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2

Vitamin C 10 mg

Biotin 10

Nicotinic acid amide 1.7 mg

Folic Acid 50

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above-mentioned vitamins and minerals is comparatively comparatively mixed with the granules according to the preferred embodiment. However, the blending ratio may be arbitrarily changed, and the above components are mixed according to the ordinary granule preparation method, Can be prepared and used in the manufacture of a health functional food composition according to a conventional method.

Formulation example  7. Manufacture of functional beverages

 1,000 mg of the extract powder obtained in Example 1

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to the flask to obtain a total of 900 mL

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (8)

Characterized in that the extract comprises an extract obtained by mixing the herbal extract, the scent, the moss, the licorice, the root, and the crust according to a weight ratio of 1: 0.2-0.8: 0.05-0.3: 0.05-0.3: 0.2-0.8: 0.2-0.8 A pharmaceutical composition for preventing or treating pain. delete 2. The method according to claim 1, wherein the perfume, fragrance, vinegar, licorice, root, and crust are mixed in a weight ratio of 1: 0.4-0.6: 0.05-0.15: 0.05-0.15: 0.4-0.6: 0.4-0.6. &Lt; / RTI &gt; The pharmaceutical composition for preventing or treating pain according to claim 1, wherein the mixed extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether or a mixed solvent thereof. The pharmaceutical composition for preventing or treating pain according to claim 1, wherein the pain is pain caused by administration of reserpine. The pharmaceutical composition for preventing or treating pain according to claim 1, wherein the pain is fibromyalgia. Characterized in that the extract comprises an extract obtained by mixing the herbal extract, the scent, the moss, the licorice, the root, and the crust according to a weight ratio of 1: 0.2-0.8: 0.05-0.3: 0.05-0.3: 0.2-0.8: 0.2-0.8 A food composition for preventing or ameliorating pain. delete

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