KR101861784B1 - A composition for improving, preventing and treating pulmonary disease comprising herb extract - Google Patents
A composition for improving, preventing and treating pulmonary disease comprising herb extract Download PDFInfo
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- KR101861784B1 KR101861784B1 KR1020160113233A KR20160113233A KR101861784B1 KR 101861784 B1 KR101861784 B1 KR 101861784B1 KR 1020160113233 A KR1020160113233 A KR 1020160113233A KR 20160113233 A KR20160113233 A KR 20160113233A KR 101861784 B1 KR101861784 B1 KR 101861784B1
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Abstract
본 발명은 불면증 개선, 예방 또는 치료용 조성물에 관한 것으로 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유함으로써, 불면증을 개선, 예방 또는 치료할 수 있다. 또한, 독성이 없으므로 식품의 형태로 섭취할 수 있다.The present invention relates to a composition for insomnia improvement, prevention or treatment of Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and DOUCY (Glycine max) number, improving, preventing or treating insomnia by containing the mixed extract consisting of the active ingredient in have. In addition, since it is not toxic, it can be ingested in the form of food.
Description
본 발명은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유하는 불면증 개선, 예방 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for improving, preventing or treating insomnia containing, as an active ingredient, a mixed extract consisting of Salvia miltiorrhiza, Gardenia jasminoides and Glycine max .
불면증은 상당히 오랜 시간 동안 지속되는 잠의 양 및 질의 면에서 불만족인 상태이다. 상기 불면증은 임상적으로 흔하며 종종 발생하는 질병으로, 그것의 임상적 증상은 잠 드는데 어려움, 잠에서 쉽게 깨는 것, 깬 후에 다시 잠들 수 없는 것, 아침에 너무 일찍 깨는 것, 쪽잠, 밤새 자지 못하는 것, 깊게 자지 못하는 것 또는 졸리지 않는 것을 나타내며, 꿈이 많은 것을 동반할 수 있다. Insomnia is dissatisfied with the amount and quality of sleep that lasts for quite some time. The insomnia is a clinically common and often occurring disease, its clinical symptoms include difficulty sleeping, easy waking up from sleep, unable to sleep again after awakening, prematurely waking up in the morning, sleeping, sleeping overnight , Not sleeping deeply or not sleepy, and can accompany many dreams.
또한, 불면증은 잠에서 깬 후 불편함, 피로, 정신적 활동의 효율 저하 또는 사회적 기능의 방해를 야기할 수 있다. In addition, insomnia can lead to discomfort, fatigue, decreased efficiency of mental activity, or disruption of social function after waking up from sleep.
20세 사람들의 약 20%가 수면 장애로 고통받으며; 이는 55세에서 50%로, 80 세에서 90%로 증가한다. 최근 통계 데이터에 의하면 42.5%가 넘는 사람들이 서로 다른 정도로 수면 장애로 고통받으며, 불면증의 상태는 10%-20%까지 다다른다.About 20% of people aged 20 suffer from sleep disturbances; This increases from 55 to 50%, from 80 to 90%. According to recent statistical data, more than 42.5% of people suffer from sleep disturbances to different degrees, and the state of insomnia reaches 10% -20%.
최근 몇 년 동안, 사람들의 사회적 경쟁 압력, 특히 매우 긴장한 근로 및 연구의 증가와 함께 질병의 상태는 증가하고 있으며, 이는 환자들의 생활, 근로 및 연구의 질에 심각하게 영향을 미치고, 근로, 연구 및 사회적 커뮤니케이션에 부정적인 영향을 야기하였다. In recent years, the societal competitive pressures of people, especially the increasingly tense work and research, have increased the status of the disease, which has seriously affected the quality of life, work and research of the patients, This has had a negative impact on social communication.
교통사고의 45%가 수면 부족과 관련되며, 작업 부상의 50%가 수면 부족과 관련되고, 만성 불면증의 사고 위험은 정상인의 그것에 대하여 4.5 배이다(Da JING, China Medical Tribune, March 20, 2003, Issue 853).45% of traffic accidents are associated with sleep deprivation, 50% of work injuries are associated with sleep deprivation, and the risk of accidents with chronic insomnia is 4.5 times that of normal people (Da Jing, China Medical Tribune, March 20, 2003, Issue 853).
생활의 가속화된 속도, 증가한 일과 압력 등에 의해 불면증 환자들이 점점 더 증가하고 있으므로 효율적으로 불면증을 예방하고 치료할 체료제가 점점 요구되고 있다. Because of the increasing speed of life, increasing workload and pressure, patients with insomnia are increasing more and more.
최근 몇 년 간의 연구들은 진정제 단독의 효율이 좋지 않고, 특히 화학적으로 합성된 약들의 독성 및 부작용이 점점 더 분명해져, 장기 복용 시 반동성 불면증, 불안, 금단증상, 내성, 의존성 등의 부작용이 심각하다.In recent years, studies have shown that the efficacy of sedation alone is poor, especially toxic and adverse effects of chemically synthesized drugs are becoming increasingly serious, and side effects such as rebound insomnia, anxiety, withdrawal symptoms, tolerance and dependence are severe .
이에, 최근에 천연물에서 유래된 불면증 치료제에 대한 개발이 진행 진행되Thus, the development of a therapeutic agent for insomnia derived from natural materials has been progressed recently
고 있으며, 그 결과 미국 및 영국에서 Lemon balm, Valerian, Hops, Passionflower, Kava kava, Sprouted oats, Lavender, Chamomile, St. Johns wort, Jasmine 등의 천연물 치료제가 개발되어 널리 시판 활용되고 있다. 물론, 동양에서 자생하는 천연물중 산조인, 용안육, 후박, 원지, 조구등, 감국, 홍삼 등에 대한 불면개선효과에 대한 연구가 이루어졌으나 상대적으로 연구 및 상품 개발이 적은 편이다.As a result, Lemon balm, Valerian, Hops, Passionflower, Kava kava, Sprouted oats, Lavender, Chamomile, Johns wort, and Jasmine have been developed and widely used in the market. Of course, research on the improvement of insomnia among the natural products of oriental origin, such as Sanjian, Yongan, Yakju, Chungcheong, Chungkuk, Red ginseng and so on, has been studied, but relatively little research and product development is done.
본 발명의 목적은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유함으로써 불면증을 예방 또는 치료할 수 있는 약학 조성물에 관한 것이다.An object of the present invention relates to Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and DOUCY (Glycine max) a pharmaceutical composition which can prevent or treat insomnia by containing the mixed extract consisting of an active substance.
또한, 본 발명의 다른 목적은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유함으로써 불면증을 예방 또는 치료할 수 있는 식품 조성물에 관한 것이다.It is another object of the present invention relates to a Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and a food composition that can prevent or treat insomnia DOUCY by containing the mixed extract consisting of an active ingredient in (Glycine max).
상기한 목적을 달성하기 위한 본 발명의 불면증 예방 또는 치료용 약학 조성물은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유할 수 있다.In order to achieve the above object, the pharmaceutical composition for preventing or treating insomnia according to the present invention may contain, as an active ingredient, a mixed extract of Salvia miltiorrhiza, Gardenia jasminoides and Glycine max .
상기 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)는 1 : 1-15 : 1-10의 중량비, 바람직하게는 1 : 1-10 : 1-5의 중량비로 혼합될 수 있다. Salvia miltiorrhiza, Gardenia jasminoides and Glycine max may be mixed in a weight ratio of 1: 1-15: 1-10, preferably 1: 1-10: 1-5. .
상기 혼합 추출물은 물, 탄소수 1 내지 4의 저급알코올, 에틸렌글리콜, 에틸에테르 또는 이들의 혼합용매로 추출된 것일 수 있다.The mixed extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof.
또한, 상기한 다른 목적을 달성하기 위한 본 발명의 불면증 예방 또는 개선용 식품 조성물은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유할 수 있다.Further, insomnia prevention or food composition for the improvement of the present invention to achieve another object above may contain a mixed extract consisting of Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and DOUCY (Glycine max), as an active ingredient .
상기 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)는 1 : 1-15 : 1-10의 중량비로 혼합될 수 있다.The Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and DOUCY (Glycine max) is from 1: can be mixed in a weight ratio of 1-10: 1-15.
본 발명의 단삼, 치자 및 두시로 이루어진 혼합 추출물을 유효성분으로 함유하는 불면증 개선, 예방 또는 치료용 조성물은 각성 시간을 줄이고 REM 수면 시간 및 NREM 수면 시간을 증가시키므로 불면증을 개선, 예방 또는 치료시키는 효능이 매우 뛰어나 경쟁력 있는 식품 조성물 및 의약 조성물의 제조에 효과적이다. The composition for improving, preventing or treating insomnia containing the mixed extract of Danshen, Gardenia, and Dassia of the present invention as an active ingredient can reduce the awakening time and increase the REM sleep time and the NREM sleep time, Is very effective in the production of competitive food compositions and pharmaceutical compositions.
도 1은 단백질 칩에 기반한 결합분석 시스템을 위한 단백질 칩에 고정된 Cy5-Tryptamine 및 5-HT2c 수용체의 농도별 5-HT2c 수용체의 결합능을 측정한 도면이다.
도 2는 단백질 칩에 기반한 결합분석 시스템을 이용하여 실시예 1에 따라 제조된 추출물의 농도에 따른 5-HT2c 수용체에 대한 길항효과를 나타낸 도면이다.FIG. 1 is a graph showing the binding potency of 5-HT2c receptor for each concentration of Cy5-Tryptamine and 5-HT2c receptor immobilized on a protein chip for a binding assay system based on a protein chip.
FIG. 2 is a graph showing the antagonistic effect on the 5-HT 2c receptor according to the concentration of the extract prepared according to Example 1 using a protein chip-based binding assay system.
본 발명은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물을 유효성분으로 함유하는 불면증 개선, 예방 또는 치료용 조성물에 관한 것이다.
The present invention relates to a composition for improving, preventing or treating insomnia containing, as an active ingredient, a mixed extract consisting of Salvia miltiorrhiza, Gardenia jasminoides and Glycine max .
이하, 본 발명을 상세하게 설명한다. Hereinafter, the present invention will be described in detail.
본 발명의 불면증 개선, 예방 또는 치료용 조성물은 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)로 이루어진 혼합 추출물(CJS03이라 함)을 유효성분으로 함유한다.The composition for improving, preventing or treating insomnia according to the present invention contains as an active ingredient a mixed extract (called CJS03) consisting of Salvia miltiorrhiza, Gardenia jasminoides and Glycine max .
상기 단삼(Salvia miltiorrhiza)은 꿀풀과에 속하는 다년생 초본식물로서, 항균작용, 월경통생리불순 및 산후의 하복부 통증, 만성간염, 간기능 장애, 간경변증의 초기 증상에도 효능이 있다. Salvia miltiorrhiza is a perennial herbaceous plant belonging to the family Lamiaceae. It is also effective in the early symptoms of antimicrobial activity, dysmenorrhea, abdominal pain in the postpartum, chronic hepatitis, liver dysfunction, and liver cirrhosis.
또한, 상기 치자(Gardenia jasminoides)는 꼭두서니과에 속하는 상록활엽관목으로서, 염증성질환, 간염, 황달, 토혈 등의 증상에 이용된다.In addition, the gardenia jasminoides is an evergreen broad-leaved shrub belonging to the madder family, and is used for symptoms such as inflammatory diseases, hepatitis, jaundice, and hematemesis.
또한, 상기 두시(Glycine max)는 콩과의 콩(Glycine max Merrill)의 씨를 삶아서 발효시킨 것으로서, 풍한, 풍열에 쓰며, 번민, 울체된 기운을 풀어 주고 소화를 돕는다.Also, Glycine max is a fermented soybean bean (Glycine max Merrill) seed, which is fermented by the seeds. It is used for a rich, radiant heat, relieves agitation, and helps digestion.
상기 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)는 1 : 1-15 : 1-10의 중량비, 바람직하게는 1 : 1-10 : 1-5의 중량비, 더욱 바람직하게는 1 : 1-5 : 1-3의 중량비로 혼합된다. 단삼을 기준으로 치자 및 두시의 함량이 상기 하한치 미만인 경우에는 불면증을 개선, 예방 또는 치료하는 효과가 각 한약재를 단독으로 사용하는 경우보다도 낮을 수 있으며, 상기 상한치 초과인 경우에는 불면증을 개선, 예방 또는 치료하는 효과가 저하될 수 있다.The Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and DOUCY (Glycine max) is from 1: 1-15: Preferably, a weight ratio of 1-5, and more preferably the weight ratio of 1-10, preferably 1: 1-10 1: 1-5: 1-3. In the case where the content of ginger and persimmon is less than the lower limit based on the ginseng root, the effect of improving, preventing or treating insomnia may be lower than that of each herbal medicine alone. If the content is above the upper limit, The therapeutic effect may be deteriorated.
상기 단삼, 치자 및 두시를 단독으로 사용하는 경우에는 단삼, 치자 및 두시를 함께 사용하는 경우에 비하여 1.5 내지 20배로 낮은 효능을 보인다. In the case of using alone, ginseng, gardenia, and two oysters, the efficacy is 1.5 to 20 times lower than in the case of using ginseng, gardenia, and two oysters.
상기 단삼(Salvia miltiorrhiza), 치자(Gardenia jasminoides) 및 두시(Glycine max)의 혼합물은 추출용매와 1 : 5 내지 25, 바람직하게는 1 : 8 내지 15의 중량비로 혼합하여 60 내지 90 ℃에서 추출하여 추출물을 제조한다. 상기 단삼, 치자 및 두시와, 추출용매의 중량비가 상기 범위를 벗어나는 경우에는 추출물에 단삼, 치자 및 두시 혼합물의 유효성분이 적은 양으로 추출될 수 있다.Extracted at 60 to 90 ℃ were mixed at a weight ratio of 8 to 15: the Danshen (Salvia miltiorrhiza), gardenia (Gardenia jasminoides), and mixtures of DOUCY (Glycine max) is the extraction solvent, and from 1: 5 to 25, preferably 1 The extract is prepared. When the weight ratio of the ginseng root, the grooming root and the extracting solvent is out of the above range, the effective component of the ginseng root, the grooming ginseng root and the mixture of the ginseng root can be extracted in a small amount.
상기 각 추출물을 추출하는 추출용매는 물, 탄소수 1 내지 4의 저급알코올, 에틸렌글리콜, 에틸에테르 또는 이들의 혼합용매이다. 상기 추출용매로는 특별히 한정하는 것은 아니지만 물로 추출된 추출물이 불면증의 개선, 예방 또는 치료에 바람직하게 작용한다. The extraction solvent for extracting each of the above extracts is water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. The extraction solvent is not particularly limited, but an extract extracted with water is preferably used for improving, preventing or treating insomnia.
본 명세서에서 단삼, 치자 및 두시의 혼합물을 언급하면서 사용되는 용어 '추출물'은 추출용매를 처리하여 얻은 조추출물뿐만 아니라 단삼, 치자 및 두시의 혼합 추출물의 가공물도 포함한다. 예를 들어, 단삼, 치자 및 두시의 혼합 추출물은 감압 증류 및 동결 건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조될 수 있다.The term 'extract' used herein to refer to a mixture of red ginseng, gardenia, and persimmon includes not only the crude extract obtained by treating the extraction solvent, but also the components of the mixed extract of red ginseng, gardenia and persimmon. For example, the mixed extract of Danshen, Gardenia and Dixie can be prepared in powder form by an additional process such as vacuum distillation and lyophilization or spray drying.
또한, 본 발명의 단삼, 치자 및 두시의 혼합 추출물은 광의로는 단삼, 치자 및 두시의 혼합물을 동물에게 투여할 수 있도록 제형화된 단삼, 치자 및 두시의 혼합 가공물, 예컨대, 단삼, 치자 및 두시의 혼합 분말도 포함하는 의미를 갖는다. 비록 본 발명에서 단삼, 치자 및 두시의 혼합물로 실험을 진행하긴 하였으나, 단삼, 치자 및 두시의 혼합 가공물과 같은 형태로도 목적하는 효과를 달성할 수 있음은 당업자라면 예상가능할 것이다.In addition, the mixed extracts of Danshen, Gardenia, and Dassia of the present invention can be prepared by mixing a mixture of Danshen, Gardenia, and Dashi, which is formulated so that a mixture of Danshen, And the like. Although the present invention has been carried out with a mixture of ginseng, gardenia, and persimmon, it can be expected that those skilled in the art can achieve the desired effects in the form of a mixture of ginseng, gardenia and persimmon.
한편, 본 명세서에서 용어 '유효성분으로 함유하는'이란 단삼, 치자 및 두시의 혼합 추출물의 효능 또는 활성을 달성하는 데 충분한 양을 포함하는 것을 의미한다. 일예로, 상기 단삼, 치자 및 두시의 혼합 추출물은 10 내지 1500 ㎍/㎖, 바람직하게는 100 내지 1000 ㎍/㎖의 농도로 사용된다. 단삼, 치자 및 두시의 혼합 추출물은 천연물로서 과량 투여하여도 인체에 부작용이 없으므로 본 발명의 조성물 내에 포함되는 단삼, 치자 및 두시의 혼합 추출물의 양적 상한은 당업자가 적절한 범위 내에서 선택하여 실시할 수 있다.
In the present specification, the term " comprising as an active ingredient " is meant to include an amount sufficient to achieve the efficacy or activity of the mixed extract of Danshen, Gardenia and Dassia. For example, the mixed extracts of Phellinus linteus, Grasshopper, and Doshisha are used at a concentration of 10 to 1500 μg / ml, preferably 100 to 1000 μg / ml. Since the mixed extracts of Danshen, Gardenia and Dixi are natural products, there is no adverse effect on the human body even when they are administered in an excessive amount. Therefore, the quantitative upper limit of the mixed extract of Danshen, Gardenia, and Dassia contained in the composition of the present invention can be selected by a person skilled in the art have.
본 발명의 약제학적 조성물은 상기 유효 성분 이외에 약제학적으로 적합하고 생리학적으로 허용되는 보조제를 사용하여 제조될 수 있으며, 상기 보조제로는 부형제, 붕해제, 감미제, 결합제, 피복제, 팽창제, 윤활제, 활택제 또는 향미제 등을 사용할 수 있다.The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.
상기 약제학적 조성물은 투여를 위해서 상기 기재한 유효 성분 이외에 추가로 약제학적으로 허용 가능한 담체를 1종 이상 포함하여 약제학적 조성물로 바람직하게 제제화할 수 있다.The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.
상기 약제학적 조성물의 제제 형태는 과립제, 산제, 정제, 피복정, 캡슐제, 좌제, 액제, 시럽, 즙, 현탁제, 유제, 점적제 또는 주사 가능한 액제 등이 될 수 있다. 예를 들어, 정제 또는 캡슐제의 형태로의 제제화를 위해, 유효 성분은 에탄올, 글리세롤, 물 등과 같은 경구, 무독성의 약제학적으로 허용 가능한 불활성 담체와 결합될 수 있다. 또한, 원하거나 필요한 경우, 적합한 결합제, 윤활제, 붕해제 및 발색제 또한 혼합물로 포함될 수 있다. 적합한 결합제는 이에 제한되는 것은 아니나, 녹말, 젤라틴, 글루코스 또는 베타-락토오스와 같은 천연 당, 옥수수 감미제, 아카시아, 트래커캔스 또는 소듐올레이트와 같은 천연 및 합성 검, 소듐 스테아레이트, 마그네슘 스테아레이트, 소듐 벤조에이트, 소듐 아세테이트, 소듐 클로라이드 등을 포함한다. 붕해제는 이에 제한되는 것은 아니나, 녹말, 메틸 셀룰로스, 아가, 벤토니트, 잔탄 검 등을 포함한다.The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.
액상 용액으로 제제화되는 조성물에 있어서 허용 가능한 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로즈 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile solutions suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.
더 나아가 해당분야의 적절한 방법으로 Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA에 개시되어 있는 방법을 이용하여 각 질환에 따라 또는 성분에 따라 바람직하게 제제화할 수 있다.Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.
본 발명의 약제학적 조성물은 경구 또는 비경구로 투여할 수 있고, 비경구 투여인 경우에는 정맥내 주입, 피하 주입, 근육 주입, 복강 주입, 경피 투여 등으로 투여할 수 있으며, 바람직하게는 경구 투여이다.The pharmaceutical composition of the present invention can be administered orally or parenterally. In the case of parenteral administration, the composition can be administered by intravenous injection, subcutaneous injection, muscle injection, intraperitoneal injection, transdermal administration, etc., .
본 발명의 약제학적 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사는 소망하는 치료 또는 예방에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다. 본 발명의 바람직한 구현예에 따르면, 본 발명의 약제학적 조성물의 1일 투여량은 0.001-10 g/kg이다.The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Usually, a skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g / kg.
본 발명의 약제학적 조성물은 약제학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때 제형은 오일 또는 수성 매질중의 용액, 현탁액 또는 유화액 형태이거나 엑스제, 분말제, 과립제, 정제 또는 캅셀제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수 있다.The pharmaceutical composition of the present invention can be prepared in unit dose form by formulating it with a pharmaceutically acceptable carrier and / or excipient or can be manufactured by inserting it into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.
또한, 본 발명은 단삼, 치자 및 두시의 혼합 추출물을 유효성분으로 함유하는 불면증의 개선, 예방 또는 치료용 식품 조성물을 제공한다.In addition, the present invention provides a food composition for improving, preventing or treating insomnia containing as an active ingredient a mixed extract of ginseng, gardenia, and persimmon.
본 발명에 따른 식품 조성물은 상기 약제학적 조성물과 동일한 방식으로 제제화되어 기능성 식품으로 이용하거나, 각종 식품에 첨가할 수 있다. 본 발명의 조성물을 첨가할 수 있는 식품으로는 예를 들어, 음료류, 알코올 음료류, 과자류, 다이어트바, 유제품, 육류, 초코렛, 피자, 라면, 기타 면류, 껌류, 아이스크림류, 비타민 복합제, 건강보조식품류 등이 있다.The food composition according to the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectioneries, diet bars, dairy products, meat, chocolates, pizza, ram noodles, other noodles, gums, ice cream, .
본 발명의 식품 조성물은 유효성분으로서 단삼, 치자 및 두시의 혼합 추출물뿐만 아니라, 식품 제조 시에 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함한다. 상술한 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수 있다. 예컨대, 본 발명의 식품 조성물이 드링크제와 음료류로 제조되는 경우에는 본 발명의 단삼, 치자 및 두시의 혼합 추출물 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 및 각종 식물 추출액 등을 추가로 포함시킬 수 있다.The food composition of the present invention may contain, as an active ingredient, components commonly used in the manufacture of foods as well as mixed extracts of ginseng, gardenia, and two oysters, and examples thereof include proteins, carbohydrates, fats, nutrients, And flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, various plant extracts and the like are added in addition to the mixed extract of Danshen, .
본 발명은 상기 단삼, 치자 및 두시의 혼합 추출물을 유효성분으로 포함하는 불면증의 개선, 예방 또는 치료용 식품 조성물을 포함하는 건강기능식품을 제공한다. 건강기능식품이란, 단삼, 치자 및 두시의 혼합 추출물을 음료, 차류, 향신료, 껌, 과자류 등의 식품소재에 첨가하거나, 캡슐화, 분말화, 현탁액 등으로 제조한 식품으로, 이를 섭취할 경우 건강상 특정한 효과를 가져오는 것을 의미하나, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용시 발생할 수 있는 부작용 등이 없는 장점이 있다. 이와 같이 하여 얻어지는 본 발명의 건강기능식품은, 일상적으로 섭취하는 것이 가능하기 때문에 매우 유용하다. 이와 같은 건강기능식품에 있어서의 단삼, 치자 및 두시의 혼합 추출물의 첨가량은, 대상인 건강기능식품의 종류에 따라 달라 일률적으로 규정할 수 없지만, 식품 본래의 맛을 손상시키지 않는 범위에서 첨가하면 되며, 대상 식품에 대하여 통상 0.01 내지 50 중량%, 바람직하기로는 0.1 내지 20 중량%의 범위이다. 또한, 환제, 과립제, 정제 또는 캡슐제 형태의 건강기능식품의 경우에는 통상 0.1 내지 100 중량% 바람직하기로는 0.5 내지 80 중량%의 범위에서 첨가하면 된다. 한 구체예에서, 본 발명의 건강기능식품은 환제, 정제, 캡슐제 또는 음료의 형태일 수 있다.The present invention provides a health functional food comprising the food composition for improving, preventing or treating insomnia comprising the mixed extract of Danshen, Gardenia and Dassia as an active ingredient. Health functional foods are foods prepared by adding mixed extracts of Dansam, Gardenia and Dooshi to food materials such as beverages, tea, spices, gum and confectionery, or encapsulated, powdered or suspended, Unlike general medicines, it has the advantage that it does not have side effects that can occur when a drug is taken for a long time by using the food as a raw material. The health functional food of the present invention thus obtained is very useful because it can be ingested routinely. The amount of the mixed extract of ginseng, gardenia and persimmon in such a health functional food can not be uniformly determined depending on the kind of health functional food to be added, but may be added within a range that does not impair the original taste of the food, Is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight, based on the target food. In the case of health functional foods in the form of pills, granules, tablets or capsules, they may be added usually in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.
또한, 본 발명은 불면증의 개선, 예방 또는 치료용 의약 또는 식품의 제조를 위한 단삼, 치자 및 두시의 혼합 추출물의 용도를 제공한다. 상기한 바와 같이 단삼, 치자 및 두시의 혼합 추출물은 불면증의 개선, 예방 또는 치료를 위한 용도로 이용될 수 있다.In addition, the present invention provides the use of mixed extracts of ginseng, gardenia and persimmon for the preparation of medicaments or foods for improving, preventing or treating insomnia. As described above, the mixed extract of Danshen, Gardenia and Dassia can be used for improving, preventing or treating insomnia.
또한, 본 발명은 포유동물에게 유효량의 단삼, 치자 및 두시의 혼합 추출물을 투여하는 것을 포함하는 불면증의 개선, 예방 또는 치료 방법을 제공한다.The present invention also provides a method for the amelioration, prevention or treatment of insomnia comprising administering to a mammal an effective amount of ginseng, gardenia, and a mixed extract of ginseng.
여기에서 사용된 용어 "포유동물"은 치료, 관찰 또는 실험의 대상인 포유동물을 말하며, 바람직하게는 인간을 말한다.The term "mammal " as used herein refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.
여기에서 사용된 용어 "유효량"은 연구자, 수의사, 의사 또는 기타 임상의에 의해 생각되는 조직계, 동물 또는 인간에서 생물학적 또는 의학적 반응을 유도하는 유효 성분 또는 약학적 조성물의 양을 의미하는 것으로, 이는 해당 질환 또는 장애의 증상의 완화를 유도하는 양을 포함한다. 본 발명의 유효 성분에 대한 유효량 및 투여횟수는 원하는 효과에 따라 변화될 수 있다. 그러므로, 투여될 최적의 투여량은 당업자에 의해 쉽게 결정될 수 있으며, 질환의 종류, 질환의 중증도, 조성물에 함유된 유효성분 및 다른 성분의 함량, 제형의 종류, 및 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여 시간, 투여 경로 및 조성물의 분비율, 치료기간, 동시 사용되는 약물을 비롯한 다양한 인자에 따라 조절될 수 있다. 본 발명의 예방, 치료 또는 개선 방법에 있어서, 성인의 경우, 단삼, 치자 및 두시의 혼합 추출물을 1일 1회 내지 수회 투여시, 0.001 g/kg 내지 10 g/kg의 용량으로 투여하는 것이 바람직하다.As used herein, the term "effective amount" refers to the amount of active ingredient or pharmaceutical composition that elicits a biological or medical response in a tissue system, animal, or human, as contemplated by a researcher, veterinarian, physician or other clinician, ≪ / RTI > inducing a reduction of the symptoms of the disease or disorder. The effective amount and the administration frequency for the active ingredient of the present invention can be changed according to the desired effect. Thus, the optimal dosage to be administered can be readily determined by those skilled in the art and will vary with the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, The age, body weight, sex, diet, time of administration, route of administration and fraction of the composition, duration of treatment, concurrent medication, and the like. In the prevention, treatment, or improvement method of the present invention, it is preferable that the dose of 0.001 g / kg to 10 g / kg is administered when the mixed extract of Danshen, Do.
본 발명의 치료방법에서 단삼, 치자 및 두시의 혼합 추출물을 유효 성분으로 포함하는 조성물은 경구, 직장, 정맥내, 동맥내, 복강내, 근육내, 흉골내, 경피, 국소, 안구내 또는 피내 경로를 통해 통상적인 방식으로 투여할 수 있다.
In the treatment method of the present invention, the composition comprising the mixed extract of Danshen, Gardenia and Dixi as an active ingredient can be administered orally, rectally, intravenously, intraarterally, intraperitoneally, intramuscularly, intrasternally, transdermally, topically, In a conventional manner.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시하나, 하기 실시예는 본 발명을 예시하는 것일 뿐 본 발명의 범주 및 기술사상 범위 내에서 다양한 변경 및 수정이 가능함은 당업자에게 있어서 명백한 것이며, 이러한 변형 및 수정이 첨부된 특허청구범위에 속하는 것도 당연한 것이다.It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.
대조구 1. 단삼 단독 사용
한국신약 주식회사(Han Kook Shin Yak Pharmaceutical Co., Ltd., Nonsan, Korea)에서 구입한 단삼과 물을 1 : 10의 중량비로 혼합하여 90 ℃에서 3시간 동안 열수 추출하여 단삼 추출물을 수득하였다.
The mixture was mixed at a weight ratio of 1:10 and the mixture was subjected to hot water extraction at 90 ° C for 3 hours to obtain the extract of Radix Salviae Radix.
대조구 2. 치자 단독 사용Control 2.
상기 대조구 1과 동일하게 제조하되, 단삼 대신 치자를 사용하여 치자 추출물을 수득하였다.
The same procedure as in
대조구 3. 두시 단독 사용Control 3. Doosan sole use
상기 대조구 1과 동일하게 제조하되, 단삼 대신 두시를 사용하여 두시 추출물을 수득하였다.
The control was prepared in the same manner as in
실시예 1. 단삼 : 치자 : 두시 = 1 : 1.66 : 1.25의 중량비Example 1: Weight ratio of ginseng: gardenia: dashi = 1: 1.66: 1.25
한국신약 주식회사(Han Kook Shin Yak Pharmaceutical Co., Ltd., Nonsan, Korea)에서 구입한 단삼, 치자 및 두시를 1 : 1.66 : 1.25의 중량비로 혼합한 후 상기 혼합물과 물을 1 : 10의 중량비로 혼합하여 90 ℃에서 3시간 동안 열수 추출함으로써 단삼, 치자 및 두시의 혼합 추출물을 수득하였다.
Danshen, gardenia, and two oysters purchased from Han Kook Shin Yak Pharmaceutical Co., Ltd., Nonsan, Korea were mixed at a weight ratio of 1: 1.66: 1.25, and the mixture and water were mixed at a weight ratio of 1:10 Mixed and extracted with hot water at 90 캜 for 3 hours to obtain a mixed extract of ginseng, gardenia and persimmon.
실시예 2. 단삼 : 치자 : 두시 = 1 : 5 : 2의 중량비Example 2 Weight ratio of ginseng: gardenia: dashi = 1: 5: 2
상기 실시예 1과 동일하게 실시하되, 단삼, 치자 및 두시를 1 : 5 : 2의 중량비로 혼합하여 혼합 추출물을 수득하였다.
The same procedure as in Example 1 was carried out except that the mixture of Danshen, Gardenia, and Dashi was mixed at a weight ratio of 1: 5: 2 to obtain a mixed extract.
비교예 1. 단삼 : 치자 : 두시 = 1 : 20 : 15의 중량비Comparative Example 1. Weight ratio of ginseng: gardenia: dashi = 1: 20: 15
상기 실시예 1과 동일하게 실시하되, 단삼, 치자 및 두시를 1 : 20 : 15의 중량비로 혼합하여 혼합 추출물을 수득하였다.
The same procedure as in Example 1 was carried out except that the mixture of Danshen, Gardenia, and Dashi was mixed at a weight ratio of 1:20:15 to obtain a mixed extract.
비교예Comparative Example 2. 2. 단삼Sansam : 치자 : : Gardener: 두시Dosh = 1 : 0.5 : 0.5의 중량비 = 1: 0.5: 0.5
상기 실시예 1과 동일하게 실시하되, 단삼, 치자 및 두시를 1 : 0.5 : 0.5의 중량비로 혼합하여 혼합 추출물을 수득하였다.
The same procedure as in Example 1 was carried out except that Danshen, Gardenia and Dassia were mixed at a weight ratio of 1: 0.5: 0.5 to obtain a mixed extract.
비교예 3. 치자 : 두시 = 1 : 0.75의 중량비Comparative Example 3: Weight ratio of gardenia: poise = 1: 0.75
상기 실시예 1과 동일하게 실시하되, 치자 및 두시를 1 : 0.75의 중량비로 혼합하여 혼합 추출물을 수득하였다(상기 중량비는 실시예 1에서 사용된 치자와 두시 간의 중량비와 동일하다).
The mixture was mixed in a weight ratio of 1: 0.75 to obtain a mixed extract (the weight ratio was the same as that of the gardenia used in Example 1).
비교예 4. 단삼 : 야교등 : 두시 = 1 : 1.66 : 1.25의 중량비COMPARATIVE EXAMPLE 4 Dansam: Hachikyo, etc.: Dose = 1: 1.66: Weight ratio of 1.25
상기 실시예 1과 동일하게 실시하되, 단삼, 야교등 및 두시를 1 : 1.66 : 1.25의 중량비로 혼합하여 혼합 추출물을 수득하였다.
The same procedure as in Example 1 was carried out except that the mixture of Dansam, Hajime, etc. and two osmolytes were mixed at a weight ratio of 1: 1.66: 1.25 to obtain a mixed extract.
비교예 5. 단삼 : 치자 : 야교등 = 1 : 1.66 : 1.25의 중량비Comparative Example 5: Weight ratio of ginseng: gardenia: yakigyo etc. = 1: 1.66: 1.25
상기 실시예 1과 동일하게 실시하되, 단삼, 치자 및 야교등을 1 : 1.66 : 1.25의 중량비로 혼합하여 혼합 추출물을 수득하였다.
The same procedure as in Example 1 was carried out except that the mixture of Dansamp, Gardenia, and Hajime was mixed at a weight ratio of 1: 1.66: 1.25 to obtain a mixed extract.
<< 시험예Test Example >>
시험예Test Example 1. One. 길항효과 측정Antagonistic effect measurement
세로토닌(5-HT) 2 수용체는 수면에 중요한 역할을 하며, 5-HT2a, 5-HT2b 및 5-HT2c 수용체인 세가지 아형으로 구분된다. 서파(slow wave) 수면은 다른 아형들보다 5-HT2c 수용체를 조절하는 것이 중요하다. 상기 5-HT2c 수용체는 REM 수면(Rapid eye movement sleep)과 NREM 수면(non rapid eye movement sleep)의 조절에 중요한 역할을 하므로 5-HT2c 수용체에 대한 길항효과를 보이는 추출물을 선택하기 위하여 하기 실험을 수행한다.Serotonin (5-HT) 2 receptors play an important role in sleep and are divided into three subtypes, 5-HT2a, 5-HT2b and 5-HT2c receptors. It is important to regulate the 5-HT2c receptor in the slow wave sleep rather than in other subtypes. Since the 5-HT2c receptor plays an important role in the regulation of REM sleep (rapid eye movement sleep) and NREM sleep (non rapid eye movement sleep), the following experiment was conducted to select an antagonistic effect to the 5-HT2c receptor do.
Stock buffer로 용해되어 있는 5-HT2c 수용체(Serotonin 5-HT2c membrane preparation in HEK293 cells, Product #6110548400UA, Perkin-Elmer)를 500 ug/ml 농도에서 시작하여 Assay Buffer(50 mM Tris-HCl, 10 mM MgCl, 1 mM EDTA, 0.1% BSA pH 7.4)로 10배씩 희석하여 4 ℃에서 16시간 동안 결합시켜 단백질 칩(ProteinChip, Proteogen and Innopharmascreen Inc.) 표면에 고정시킨다. 이를 Washing Buffer(0.05% PBST)로 10분 동안 세척한 후 N2 가스를 이용하여 건조시킨 다음, 상기 건조된 5-HT2c 수용체 칩을 3% BSA blocking buffer로 실온에서 1시간 동안 반응시킨 후 Washing Buffer(0.05% PBST)로 다시 2번 세척하고 Cy5-Tryptamine(500 uM, using 30% glycerol in PBS as buffer, Peptron사)을 농도별로 첨가하여 1시간 동안 반응시킨다. 상기 반응이 수행된 단백질 칩을 PBST 및 DW로 세척시킨 후 N2 가스로 건조시킨 다음 형광 스캐너(GenePix pro A4100)를 이용하여 각 spot의 형광 값을 측정하여 5-HT2c 수용체 결합능을 GenePix pro 6.0(Axon Instruments, CA, USA)을 사용하여 분석하였다.(50 mM Tris-HCl, 10 mM MgCl 2) at 5 μg / ml in 5-HT2c receptor (Serotonin 5-HT2c membrane preparation in HEK293 cells, Product # 6110548400UA, Perkin- , 1 mM EDTA, 0.1% BSA pH 7.4) and fixed at 4 ° C for 16 hours on a protein chip (ProteinChip, Proteogen and Innopharmascreen Inc.). The dried 5-HT2c receptor chip was reacted with 3% BSA blocking buffer for 1 hour at room temperature, washed with Washing Buffer (0.05% PBST) for 10 minutes, dried using N2 gas, 0.05% PBST), and reacted with Cy5-Tryptamine (500 uM, using 30% glycerol in PBS as buffer, Peptron) for 1 hour. The protein chip on which the reaction was performed was washed with PBST and DW, dried with N2 gas, and then fluorescence values of each spot were measured using a fluorescent scanner (GenePix pro A4100) to determine 5-HT2c receptor binding ability using GenePix pro 6.0 Instruments, CA, USA).
도 1은 단백질 칩에 기반한 결합분석 시스템을 위한 단백질 칩에 고정된 Cy5-Tryptamine 및 5-HT2c 수용체의 농도별 5-HT2c 수용체의 결합능을 측정한 도면이다.FIG. 1 is a graph showing the binding potency of 5-HT2c receptor for each concentration of Cy5-Tryptamine and 5-HT2c receptor immobilized on a protein chip for a binding assay system based on a protein chip.
도 1에 도시된 바와 같이, 단백질 칩에 기반한 결합분석 시스템을 위한 Cy5-Tryptamine 및 5-HT2c 수용체의 최적 농도는 각각 500 uM 및 50 ㎍/㎖인 것을 확인하였다.As shown in FIG. 1, optimal concentrations of Cy5-Tryptamine and 5-HT2c receptors for binding assay systems based on protein chips were 500 uM and 50 μg / ml, respectively.
상기 단백질 칩에 기반한 결합분석 시스템은 한약재를 스크리닝하는데 사용된다.
The binding assay system based on the protein chip is used for screening medicinal herbs.
5-HT2c 수용체를 50 ㎍/㎖ 농도로 상기와 동일한 방법으로 단백질 칩 표면에 고정시키고 세척한 후 건조시킨 다음 상기 건조된 5-HT2cR 칩을 3% BSA blocking buffer로 실온에서 1시간 동안 반응시키고 다시 세척한 후, 대조구, 실시예 및 비교예에서 제조된 추출물 0.5 mg/ml을 상기 5-HT2cR 칩에 투입하여 37 ℃에서 1시간 동안 반응시킨다. 이후 Cy5-Tryptamine 500 uM(500 ㎍/㎖)을 첨가하여 1시간 동안 반응시키고 반응이 완료된 5-HT2cR 칩을 세척하고 건조시켜 형광 스캐너를 이용하여 각 spot의 형광 값을 측정하였다.The 5-HT2c receptor was immobilized on the surface of the protein chip at a concentration of 50 μg / ml in the same manner as described above, washed and dried. The dried 5-HT2cR chip was reacted with 3% BSA blocking buffer for 1 hour at room temperature After washing, 0.5 mg / ml of the extract prepared in the control, Examples and Comparative Examples was added to the 5-HT2cR chip and allowed to react at 37 ° C for 1 hour. Then, 500 μM Cy5-Tryptamine (500 μg / ml) was added and reacted for 1 hour. The 5-HT2cR chip was washed and dried, and fluorescence values of each spot were measured using a fluorescence scanner.
위 표 1에 나타낸 바와 같이, 본 발명의 실시예 1 및 2에 따라 제조된 추출물은 대조구 1 내지 3 및 비교예 1 내지 5에 따라 제조된 추출물에 비하여 형광 수치가 낮은 것을 확인하였다.As shown in Table 1, the extracts prepared according to Examples 1 and 2 of the present invention were confirmed to have lower fluorescence values than the extracts prepared according to
상기 형광 수치가 낮다는 것은 가장 효과적인 길항제라는 의미이므로 실시예 1 및 2의 추출물이 다른 군의 추출물에 비하여 5-HT2c 수용체에 대한 우수한 길항제인 것을 확인하였다.Since the low fluorescence value means the most effective antagonist, it was confirmed that the extracts of Examples 1 and 2 were superior antagonists to the 5-HT2c receptor as compared to the extracts of other groups.
상기 실시예 1 및 2의 추출물 중에서도 실시예 1의 추출물이 5-HT2c 수용체에 대하여 가장 우수한 길항제이므로 하기에서는 실시예 1의 추출물을 이용하여 실험을 수행한다.
Among the extracts of Examples 1 and 2, the extract of Example 1 is the most excellent antagonist for the 5-HT2c receptor, so that the experiment is carried out using the extract of Example 1 in the following.
5-HT2c 수용체를 50 ㎍/㎖ 농도로 상기와 동일한 방법으로 단백질 칩 표면에 고정시키고 세척한 후 건조시킨 다음 상기 건조된 5-HT2cR 칩을 3% BSA blocking buffer로 실온에서 1시간 동안 반응시키고 다시 세척한 후, 실시예 1에서 제조된 추출물을 농도별로 상기 5-HT2cR 칩에 투입하여 37 ℃에서 1시간 동안 반응시킨다. 이후 Cy5-Tryptamine 500 uM을 첨가하여 1시간 동안 반응시키고 반응이 완료된 5-HT2cR 칩을 세척하고 건조시켜 형광 스캐너를 이용하여 각 spot의 형광 값을 측정하였다.The 5-HT2c receptor was immobilized on the surface of the protein chip at a concentration of 50 μg / ml in the same manner as described above, washed and dried. The dried 5-HT2cR chip was reacted with 3% BSA blocking buffer for 1 hour at room temperature After washing, the extract prepared in Example 1 was added to the 5-HT2cR chip for each concentration, and reacted at 37 ° C for 1 hour. Then, 500 μM of Cy5-Tryptamine was added to react for 1 hour. The 5-HT2cR chip was washed and dried, and the fluorescence value of each spot was measured using a fluorescence scanner.
도 2는 단백질 칩에 기반한 결합분석 시스템을 이용하여 실시예 1에 따라 제조된 추출물의 농도에 따른 5-HT2c 수용체에 대한 길항효과를 나타낸 도면이다.FIG. 2 is a graph showing the antagonistic effect on the 5-HT 2c receptor according to the concentration of the extract prepared according to Example 1 using a protein chip-based binding assay system.
도 2에 도시된 바와 같이, 5-HT2c 수용체의 농도가 50 ㎍/㎖ 일 때 Cy5-Tryptamine에 대한 실시예 1의 추출물의 IC50은 197.44 mg/ml인 것을 확인하였다. 이러한 데이터는 실시예 1의 추출물이 5-HT2c 수용체와 Cy5-Tryptamine 사이의 상호작용을 차단하는 것을 의미하므로 상기 실시예 1의 추출물이 수면 구조의 조절에 유용한 물질임을 알 수 있다.
As shown in FIG. 2, the IC 50 of the extract of Example 1 against Cy5-Tryptamine was found to be 197.44 mg / ml when the concentration of 5-HT2c receptor was 50 占 퐂 / ml. These data indicate that the extract of Example 1 blocks the interaction between the 5-HT2c receptor and Cy5-Tryptamine, so that the extract of Example 1 is a useful substance for controlling the sleep surface structure.
동물실험Animal experiment
250~300 g의 Wistar 랫트(SAMTAKO, Korea, 숫컷)를 행동 실험에 사용하였다. 아크릴 케이지(45 X 60 X 25 cm3)에서 사육되었으며, 충분한 사료와 물이 공급되며 적절한 인공조도로 12시간의 낮, 밤을 조절하였다(am 8:00부터 낮). 그리고 일정한 온도(20~24 ℃) 및 습도(45~65%)를 유지시켜 주었다. 바뀐 환경에 적응하도록 일주일간 살펴보며 수면주기를 유지하고, 이상행동을 확인하였다. 250-300 g of Wistar rats (SAMTAKO, Korea, male) were used for behavioral studies. The animals were housed in an acrylic cage (45 x 60 x 25 cm 3 ), fed with sufficient feed and water, and adjusted for 12 hours of daytime and night with appropriate artificial illumination (from 8:00 am). It maintained a constant temperature (20 ~ 24 ℃) and humidity (45 ~ 65%). We observed for a week to adjust to the changed environment, maintained the sleep cycle, and confirmed abnormal behavior.
동물을 사용한 모든 실험은 MFDS's(Ministry of Food and Drug Safety) National Institute of Toxicological Research for the care and use of laboratory animals(KHUASP(SE)-14-051)를 준수하고, 대전대 동물실험윤리위원회에서 실험 방법을 승인하였다.All experiments using animals were performed in compliance with the MFDS's National Institute of Toxicological Research for the care and use of laboratory animals (KHUASP (SE) -14-051) .
실험동물은 체중변화가 일정하고 건강한 동물만을 선별하여 임의 배치법에 의해 생리식염수를 투여한 정상군, 대조구 1 내지 3의 추출물을 각각 투여한 대조군 1 내지 3 및 실시예 1의 추출물을 투여한 시료 투여군으로 나누었으며 실험군마다 8마리씩 사용하였다.Experimental animals were divided into a normal group in which physiological saline was administered by randomly selecting healthy animals with constant weight change,
시험예Test Example 2. 수면 구조 측정 2. Measurement of sleep structure
1) EEG 주입1) EEG injection
각각의 랫트들에 EEG 측정을 위한 transmitter(Data Sciences TA11CTA-F40)를 이식해야 한다. transmitter는 가죽 밑으로 심겨져 견갑골 중심에 자리잡게 되며, 두 가닥의 wire가 frontal cortex 부위에 삽입되며, 두 가닥의 wire는 후두부의 근육에 삽입되어 4가닥의 선으로 각각 EEG와 EMG 측정을 한다. 랫트의 늑골 상부에서 두골 사이를 수술용 메스로 갈라내어 frontal cortex 위의 전두골 부위가 보이게 한다. 후두부의 근육을 멸균된 주사기 바늘로 얇게 관통한 후, 바늘 사이로 wire를 통과시켜, 근육에 삽입한다. 삽입된 wire는 흔들리지 않도록 수술용 실을 이용하여 묶어준다. Cortex에 wire를 삽입하기 위하여, 전두골 위의 조직을 메스의 칼등이나 날을 이용하여 깔끔하게 제거한다. 흘러나오는 피는 멸균된 솜을 이용하여 닦아낸다. Frontal cortex에 transmitter wire를 삽입하기 위해 전두골의 좌, 우측에 medical drill을 이용하여 작은 구멍을 뚫은 다음, 피복을 벗긴 wire를 각각의 구멍을 통하여 뇌의 frontal cortex 부위에 삽입한다. 수술 후 구멍은 dental cement로 봉인하여 wire가 빠지는 것을 방지하고 추가 오염도 막도록 하였다. Dental cement가 완전히 굳으면 피부를 단단히 봉합하고, 수술 후 1주일 동안 항생제를 투여하면서 랫트를 회복시킨다.Transmitters (Data Sciences TA11CTA-F40) for EEG measurements should be implanted in each rat. The transmitter is implanted under the skin and placed in the center of the scapula. Two wires are inserted into the frontal cortex, and two wires are inserted into the muscles of the occipital area to measure EEG and EMG, respectively, with four strands of wire. The upper part of the ribs of the rat is divided into the scalp between the scalpel and the surgical scalpel to allow the frontal cortex to be seen on the frontal cortex. Thin muscles of the occiput are penetrated thinly with sterilized syringe needles, then inserted into the muscles through wires between the needles. The inserted wire is bundled using a surgical thread so that it does not shake. To insert the wire into the Cortex, clean the tissue above the frontal bone using a scalpel blade or blade. Bleed blood is wiped off with sterile cotton. To insert a transmitter wire into the frontal cortex, a small hole is drilled on the left and right sides of the frontal bone using a medical drill, and a stripped wire is inserted through the hole into the frontal cortex of the brain. After surgery, the hole was sealed with a dental cement to prevent wire from escaping and to prevent further contamination. When the dental cement is completely hardened, the skin is tightly sutured and the rats are restored by administering antibiotics for one week after surgery.
2) 추출물 투여2) Administration of extract
수술 7일 후 EEG를 측정하기 3시간 전에 대조구 1 내지 3의 추출물 및 실시예 1의 추출물(50 mg/kg씩)을 각각 경구 투여하였다. Seven days after surgery, the extracts of
3) 데이터 수집 및 분석3) Data collection and analysis
EEG 신호에 대하여, transmitter는 -0.5/+0.5 volts per units X 2이고 transmitter에서 측정된 원 신호는 0.5-20.0 Hz이며, 상기 신호는 Data Sciences analog converter에 의해 처리된다.For the EEG signal, the transmitter is -0.5 / + 0.5 volts per units X 2 and the original signal measured at the transmitter is 0.5-20.0 Hz, and the signal is processed by the Data Sciences analog converter.
상기 EEG 기록은 9:00 am에 시작되어 모든 랫트에서 12시간 동안 측정되었으며, EEG 신호는 SleepSign Ver.3 software(Kissei Comtec, Nagano, Japan)를 통해 기록된다. 수면 상태는 SleepSign Ver. 3 software (Kissei Comtec, Nagano, Japan)에 의해 자동으로 각성(Wake), REM 수면 및 NREM 수면으로 구분된다.The EEG recordings were started at 9:00 am and measured for 12 hours in all rats, and EEG signals were recorded via SleepSign Ver.3 software (Kissei Comtec, Nagano, Japan). The sleep state is SleepSign Ver. 3 software (Kissei Comtec, Nagano, Japan) automatically distinguishes between awake, REM sleep and NREM sleep.
EEG 파워 스펙트럼은 0.5 Hz 간격으로 계산되고 평균을 구하였으며, 주파수 영역이 0.65 내지 4 Hz인 델타파 활동, 6 내지 10 Hz인 세타파 활동 및 12 내지 14 Hz인 알파파 활동으로 구분된다. NREM 수면의 EEG 파워 밀도는 각 랫트의 총 EEG 파워(0-20 Hz)로부터 각 구간의 비율을 계산한 것이다.The EEG power spectrum is calculated and averaged at 0.5 Hz intervals and is divided into delta wave activity with a frequency range of 0.65 to 4 Hz, a seta activity of 6 to 10 Hz and an alpha wave activity of 12 to 14 Hz. The EEG power density of the NREM sleep surface is calculated from the ratio of each section from the total EEG power (0-20 Hz) of each rat.
3가지 수면 상태 중에서 각성은 낮은 진폭 EEG 및 높은 전압 근전도 활성을 기지며, NREM 수면은 높은 진폭과 스핀들 EEG 및 낮은 전압 근전도 활성을 가지고, REM 수면은 낮은 진폭 EEG 및 낮은 전압 근전도 활성을 갖는다. Among three sleep states, awakening has low amplitude EEG and high voltage EMG activity, NREM sleep has high amplitude, spindle EEG and low voltage EMG activity, and REM sleep has low amplitude EEG and low voltage EMG activity.
4) 통계 분석4) Statistical analysis
모든 통계 분석은 SigmaStat software(IBM® SPSS® Statistics Ver. 21)를 사용하여 실시되었으며, 분석은 ANOVA 후 Tukey test를 실시하였다.All statistical analyzes were performed using SigmaStat software (IBM ® SPSS ® Statistics Ver. 21). Tukey test was performed after ANOVA.
상기 REM 수면은 전체 수면의 20-25% 정도를 차지하며 합해서 약 90~120 분간 생기고 4~5 번에 걸쳐서 일어난다. 상기 REM 수면은 몸의 활력을 넣어주고 성장에 관계가 있으며 일정한 기억을 확실히 굳혀주는 정신적인 역할을 한다는 등의 여러 가지 학설이 있다.The REM sleep takes about 20-25% of the total sleep, resulting in about 90-120 minutes in total and occurs over 4-5 times. The REM sleep has various theories such as putting the vitality of the body, playing a role in growth, and playing a mental role in firmly fixing certain memories.
상기 NREM 수면은 4 단계로 이루어져 있으며 단계가 진행될수록 깊은 잠이 빠져들므로 피로를 풀어주고, 면역체계를 높이는 등의 신체 효과와 관계가 있다. 그러므로 NREM 수면이 수면의 질과 관련이 있는 것이다.The NREM sleep consists of four steps. As the step progresses, deep sleep falls, which is related to physical effects such as relieving fatigue and increasing the immune system. Therefore, NREM sleep is related to sleep quality.
위 표 2에 나타낸 바와 같이, 본 발명의 실시예 1에 따라 제조된 추출물을 투여한 랫트는 정상군 및 대조군 1 내지 3에 비하여 각성 시간이 줄어들고, REM 수면 및 NREM 수면 시간이 증가한 것을 확인하였다. 특히, 실시예 1에 따라 제조된 추출물을 투여한 랫트는 NREM 수면 시간이 REM 수면 시간에 비하여 더 많이 늘어난 것을 확인하였다.
As shown in Table 2, the rats administered with the extract prepared according to Example 1 of the present invention showed a decrease in awakening time and an increase in REM sleep and NREM sleep time compared to the normal group and the
하기에 본 발명의 분말을 함유하는 조성물의 제제예를 설명하나, 본 발명은 이를 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.
제제예Formulation example 1. One. 산제의Sanje 제조 Produce
실시예 1에서 얻은 추출물 분말 500 mg500 mg of the extract powder obtained in Example 1
유당 100 mg
탈크 10 mg
상기의 성분들을 혼합하고 기밀포에 충진하여 산제를 제조한다.
The above components are mixed and filled in airtight bags to prepare powders.
제제예Formulation example 2. 정제의 제조 2. Preparation of tablets
실시예 1에서 얻은 추출물 분말 300 mg300 mg of the extract powder obtained in Example 1
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mgMagnesium stearate 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.
After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.
제제예Formulation example 3. 캅셀제의 제조 3. Preparation of capsules
실시예 1에서 얻은 추출물 분말 200 mg200 mg of the extract powder obtained in Example 1
결정성 셀룰로오스 3 mgCrystalline cellulose 3 mg
락토오스 14.8 mgLactose 14.8 mg
마그네슘 스테아레이트 0.2 mgMagnesium stearate 0.2 mg
통상의 캡슐제 제조방법에 따라 상기의 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조한다.
The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.
제제예Formulation example 4. 주사제의 제조 4. Preparation of injections
실시예 1에서 얻은 추출물 분말 600 mg600 mg of the extract powder obtained in Example 1
만니톨 180 mg180 mg mannitol
주사용 멸균 증류수 2974 mgSterile sterilized water for injection 2974 mg
Na2HPO4 ,12H2O 26 mgNa 2 HPO 4 , 12H 2 O 26 mg
통상의 주사제의 제조방법에 따라 1 앰플 당 상기의 성분 함량으로 제조한다.
It is prepared by the above-mentioned component content per ampoule according to the usual injection preparation method.
제제예Formulation example 5. 5. 액제의Liquid 제조 Produce
실시예 1에서 얻은 추출물 분말 4 g4 g of the extract powder obtained in Example 1
이성화당 10 g10 g per isomer
만니톨 5 g5 g mannitol
정제수 적량Purified water quantity
통상의 액제의 제조방법에 따라 정제수에 각각의 성분을 가하여 용해시키고 레몬향을 적량 가한 다음 상기의 성분을 혼합한 다음 정제수를 가하여 전체를 정제수를 가하여 전체 100g으로 조절한 후 갈색병에 충진하여 멸균시켜 액제를 제조한다.
Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 g with purified water, To prepare a liquid agent.
제제예Formulation example 6. 과립제의 제조 6. Preparation of granules
실시예 1에서 얻은 추출물 분말 1,000 mg1,000 mg of the extract powder obtained in Example 1
비타민 혼합물 적량Vitamin mixture quantity
비타민 A 아세테이트 70 ug70 ug of vitamin A acetate
비타민 E 1.0 mgVitamin E 1.0 mg
비타민 B1 0.13 mgVitamin B1 0.13 mg
비타민 B2 0.15 mg0.15 mg of vitamin B2
비타민 B6 0.5 mgVitamin B6 0.5 mg
비타민 B12 0.2 ugVitamin B12 0.2 ug
비타민 C 10 mg
비오틴 10 ug
니코틴산아미드 1.7 mgNicotinic acid amide 1.7 mg
엽산 50 ugFolic acid 50 ug
판토텐산 칼슘 0.5 mgCalcium pantothenate 0.5 mg
무기질 혼합물 적량Mineral mixture quantity
황산제1철 1.75 mg1.75 mg of ferrous sulfate
산화아연 0.82 mg0.82 mg of zinc oxide
탄산마그네슘 25.3 mgMagnesium carbonate 25.3 mg
제1인산칼륨 15 mgPotassium monophosphate 15 mg
제2인산칼슘 55 mgSecondary calcium phosphate 55 mg
구연산칼륨 90 mgPotassium citrate 90 mg
탄산칼슘 100 mg
염화마그네슘 24.8 mgMagnesium chloride 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 과립제에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 과립제 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강기능식품 조성물 제조에 사용할 수 있다.
The composition ratio of the above-mentioned vitamins and minerals is comparatively comparatively mixed with the granules according to the preferred embodiment. However, the blending ratio may be arbitrarily changed, and the above components are mixed according to the ordinary granule preparation method, Can be prepared and used in the manufacture of a health functional food composition according to a conventional method.
제제예Formulation example 7. 기능성 음료의 제조 7. Manufacture of functional beverages
실시예 1에서 얻은 추출물 분말 1,000 mg 1,000 mg of the extract powder obtained in Example 1
구연산 1,000 mgCitric acid 1,000 mg
올리고당 100 g100 g of oligosaccharide
매실농축액 2 gPlum concentrate 2 g
타우린 1 gTaurine 1 g
정제수를 가하여 전체 900 mLPurified water was added to the flask to obtain a total of 900 mL
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1 시간 동안 85 ℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2 L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 기능성 음료 조성물 제조에 사용한다. The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 수요계층, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the compounding ratio may be arbitrarily varied depending on the regional and national preferences such as the demand level, the demanding country, and the intended use.
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Non-Patent Citations (2)
Title |
---|
Anal. Methods, 2014, vol. 6, pp. 1868-1874. |
Genomics, 2011, vol.98, pp.272-279. |
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