KR20130064882A - The composition for the prevention and treatment of diabetic or diabetic complications containing the extracts of herbal medicine - Google Patents

Abstract

PURPOSE: A pharmaceutical composition and a health functional food composition containing one or more extracts selected from the group consisting of a Panax notoginseng extract, an Angelica gigas Nakai extract, an Acanthopanax extract, a Lycii radicis cortex extract, and a safflower seed extract or a fraction thereof are provided to effectively prevent and treat diabetes or diabetes complication. CONSTITUTION: A composition for preventing or treating diabetes or diabetes complication contains one or more selected from the group consisting of a Panax notoginseng extract, an Angelica gigas Nakai extract, an Acanthopanax extract, a Lycii radicis cortex extract, and a safflower seed extract. The extracts are prepared using water, C1-C5 alcohol, and a mixture thereof. The composition contains a fraction which is prepared by fractioning one or more extracts with hexane, methylene chloride, ethyl acetate, and butanol. [Reference numerals] (AA) Panax notoginseng; (BB) Angelica gigas Nakai; (CC) Acanthopanax; (DD) Lycii radicis cortex; (EE) Safflower seed

Description

Composition for the prevention and treatment of diabetic or diabetic complications containing the extracts of herbal medicine

The present invention relates to a composition for the prevention or treatment of diabetes mellitus or diabetic complications containing herbal extracts.

Diabetes, which is rapidly increasing as the awareness of health increases due to the improvement of the living standard of the modern people and the increase of human lifespan, is a chronic disease that is attracting worldwide attention.

Diabetes mellitus is a metabolic syndrome characterized by a deficiency of insulin hormone or insulin resistance in the beta cells of the pancreas, as well as hyperglycemia resulting from both of these defects. These diabetes can be divided into insulin dependent diabetes mellitus (IDDM; Type I) and insulin independent diabetes mellitus (NIDDM; Type II) caused by insulin resistance and insulin secretion impairment. Type I diabetes is a destructive lesion of pancreatic β cells. As a result of insulin deficiency, type II diabetes is caused by decreased insulin secretion and insulin resistance. The type 2 diabetes is divided into insulin secretion predominant diabetes mellitus and insulin resistance prevalence diabetes mellitus according to the degree of involvement of these two factors.

Diabetes mellitus, chronic hyperglycemia, as well as lipid metabolism, lipid and protein metabolic disorders are also caused. The condition is diverse and is directly caused by hyperglycemia, and includes diabetic peripheral neuropathy, diabetic retinopathy, diabetic nephropathy, diabetic cataract, keratosis, and diabetic atherosclerosis in the retina, the kidney, the nervous system, and the cardiovascular system. The number of diabetic patients is steadily increasing due to changes in lifestyle such as overeating, lack of exercise, westernized eating habits, stress, drinking and smoking.

In Type I and Type II diabetes, diverse complications such as heart disease, bowel disease, ophthalmic disease, neurological disease, and stroke occur, which can lead to chronic neuropathy and cardiovascular disease due to elevated blood glucose and insulin levels over a long period of time And short-term hypoglycemia and hyperglycemia reaction cause acute complications.

Diabetes mellitus is medication, exercise and diet, and insulin medicines and various blood glucose drugs are used depending on the patient's symptoms. However, diabetes is a complex disease that is characterized by excessive glucose production in the liver, insulin resistance, and decreased ability to process glucose in muscles and fat cells. Therefore, specific treatment alone cannot prevent the occurrence of various side effects. And the use of chemicals are constantly causing side effects and patient resistance following the use of the drug. In addition, synthetic drugs such as Bristol Myers' Glucophage and GlaxoSmithKline's' Avandia ', which are currently widely prescribed in Korea, are limited in that they are not intended for the purpose of treatment and are in constant management. There is a problem in that the body can take the ability to cure the disease itself according to the continuous dose.

In recent years, researches have been made to treat diabetes using natural products that are capable of diet, have fewer side effects, and improve immunity to cure diseases of the human body, and further to prevent and treat diabetic complications. It is necessary.

The present invention is to provide a composition for preventing or treating diabetes or diabetic complications containing one or more selected from the group consisting of Samchil root extract, Angelica extract, Ogapi extract, phalanges extract and safflower seed extract.

In addition, to provide a pharmaceutical composition and health food composition for preventing or treating diabetes or diabetic complications using the composition.

The present invention provides a composition for preventing or treating diabetes or diabetic complications containing one or more selected from the group consisting of Samchil root extract, Angelica extract, Ogapi extract, phalanges extract and safflower seed extract.

The extract may be extracted with at least one selected from the group consisting of water, alcohols having 1 to 5 carbon atoms, and mixtures thereof.

Containing a fraction obtained by fractionating at least one selected from the group consisting of the three-stage root extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract with at least one solvent selected from the group consisting of hexane, methylene chloride, ethyl acetate and butanol, Provided is a composition for preventing or treating diabetes or diabetic complications.

The diabetic complication can be any one selected from the group consisting of diabetic retinopathy, diabetic cataract, diabetic keratosis, diabetic nephropathy, diabetic neuropathy, diabetic peripheral neuropathy, atherosclerosis and diabetic angiopathy .

The present invention provides a health food composition for preventing or treating diabetes or diabetic complications comprising the composition for preventing or improving diabetes or diabetes mellitus.

One or more extracts or fractions thereof selected from the group consisting of Samchil-Geun extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract according to the present invention is excellent in preventing and treating diabetes or diabetic complications, and thus the pharmaceutical composition and health food containing the same It may be utilized as a composition and the like.

Figure 1 shows the herbal medicine of the present invention, Samchil-geun, Angelica, Ogapi, phalanges and safflower seeds.
Figure 2 shows a prototype made of the extract of the present invention.

Provided is a composition for preventing or treating diabetes or diabetic complications, and health food compositions using the same, including at least one selected from the group consisting of Samchil-geun extract, Angelica extract, Ogapi extract, Phalanx extract and safflower seed extract.

Hereinafter, the present invention will be described in detail.

Drug therapy as a treatment for diabetes and diabetic complications, which are used for long term cooling, uses insulin and chemicals, which are a constant problem for side effects caused by taking medications and patient resistance. Therefore, the present invention can be used as a treatment for diabetes and diabetic complications. And while trying to find a natural product with less side effects by using the herbal extracts of the present invention can effectively absorb the peptide-based materials that are difficult to absorb confirmed that the prevention or treatment of diabetes and diabetic complications is excellent and completed the present invention.

The present invention provides a composition for preventing or treating diabetes or diabetic complications containing one or more selected from the group consisting of Samchil root extract, Angelica extract, Ogapi extract, phalanges extract and safflower seed extract.

In the Samchil root extract, Angelica extract, Ogapi extract, phalanx extract and / or safflower seed extract, the extraction method is not particularly limited, but preferably selected from the group consisting of water, alcohol having 1 to 5 carbon atoms and mixtures thereof Using the above as an extraction solvent may be obtained by a conventional plant extraction method.

The extract may be prepared according to a conventional method for producing a plant extract, preferably by pressure extraction, heat extraction, reflux extraction, hot needle extraction, ultrasonic grinding extraction, etc., but is not limited thereto.

In addition, the extract prepared as described above may then be concentrated under reduced pressure filtration or further concentrated and / or lyophilized to remove the solvent, the extract of the present invention is concentrated to dry and conventional methods It can be used to include water.

The present invention can maximize the extraction efficiency by extracting, concentrating, and drying the herbal medicine by examining the content of the indicator component.

The present invention provides a composition for the prevention or treatment of diabetes or diabetic complications containing a fraction obtained by fractionating one or more selected from the group consisting of Samchil-Geun extract, Angelica extract, Ogapi extract, Jigolpi extract and safflower seed extract.

The fractionation method is not particularly limited, but may be preferably fractionated with one or more solvents selected from the group consisting of hexane, methylene chloride, ethyl acetate and butanol.

One or more extracts and fractions thereof selected from the group consisting of the three-stage root extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract of the present invention remove the cause of diabetes and activate the functions of organs including the pancreas to secrete insulin from the pancreas It was found to improve the function and blood glucose control ability.

The diabetic complications may refer to various complications in which the hyperglycemic and protein reactions due to diabetes are irreversibly progressed to produce a final glycated product that cross-links with other proteins in the blood or tissue.

Diabetic complications of which the three-stage muscle extract, Angelica extract, Ogapi extract, phalanx extract and / or safflower seed extract and / or fraction of the present invention have a therapeutic effect are not particularly limited, but are preferably diabetic retinopathy, diabetic cataract, diabetic Corneal disease, diabetic nephropathy, diabetic neuropathy, diabetic peripheral neuropathy, atherosclerosis and diabetic angiopathy.

Diabetic complications often occur despite the normal recovery of blood sugar. Final glycosylated products are protein glycosylated products that accumulate in tissues in proportion to the concentration of hyperglycemia. They exhibit specific absorbance and fluorescence. In particular, the amino groups of proteins have cross-link reactions. Once produced, glycosylated products accumulate in tissues without being degraded even though blood sugar is normally regulated, causing abnormal changes in the structure and function of the tissues, and are known to be involved in atheroma atherosclerosis, DNA mutations, aging, Alzheimer's disease, and diabetic complications. have. As the toxicity of aminoguanidine, which is known as an inhibitor of the final glycation endogenous product, has been shown to increase, there is a need for the development of a safe, non-toxic natural product to replace it.

The present invention provides a pharmaceutical composition for preventing or treating diabetes or diabetic complications containing one or more selected from the group consisting of Samchil root extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract.

The pharmaceutical composition for the prevention and treatment of diabetes and complications of the present invention, the total weight of the composition, the extract containing one or more selected from the group consisting of the three roots extract, Angelica extract, Ogapi extract, phalanges extract and safflower seed extract 0.1 to 99 wt%.

The pharmaceutical composition containing the extract of the present invention may further include suitable carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical dosage forms of the compositions of the present invention may also be used in the form of their pharmaceutically acceptable salts and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

Pharmaceutical composition comprising one or more extracts selected from the group consisting of Samchil-Geun extract, Angelica extract, Ogapi extract, Phalanx extract and safflower seed extract according to the present invention, respectively, powders, granules, tablets, capsules, suspensions according to conventional methods Oral formulations such as emulsions, syrups, aerosols, external preparations, suppositories, and sterile injectable solutions. Examples of carriers, excipients and diluents that can be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and such solid form forms at least one excipient such as starch, calcium carbonate, sucrose or lactose. (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the composition of the present invention, including one or more extracts selected from the group consisting of Samchil-Geun Extract, Angelica Extract, Ogapi Extract, Phalanx Extract and Safflower Seed Extract, may be used as the condition and weight of the patient, degree of disease, drug form, Depending on the route and duration of administration, it may be appropriately selected by those skilled in the art.

The composition for preventing or treating diabetes or diabetic complications comprising at least one selected from the group consisting of Samchil-Geun extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract of the present invention is applied to mammals such as mice, mice, livestock, humans, etc. It can be administered by various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

The present invention provides a health food composition for preventing or improving diabetes or diabetic complications containing one or more selected from the group consisting of Samchil root extract, Angelica extract, Ogapi extract, Jigolpi extract and safflower seed extract.

The present invention includes a food supplement acceptable food additive in one or more extracts selected from the group consisting of the three muscles extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract showing the effect of preventing and treating diabetes mellitus and diabetic complications Provide health functional foods. Foods to which the extract of the present invention can be added include, for example, various foods, beverages, gums, tea, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of preventing and treating diabetes and diabetic complications. At this time, the amount of the extract in the food or beverage may be added to 0.01 to 70% by weight of the total food weight.

The health functional beverage composition of the present invention has no particular limitation on the other ingredients other than the above-mentioned extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .

In addition to the above, Samchil-geun extract, Angelica extract, Ogapi extract, phalanx extract and safflower seed extract are flavoring agents, coloring agents and neutralizing agents such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors (cheese) , Chocolate and the like), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

< Example >

Example  1: Extract of Herbal Medicine

Extracts were prepared by extracting Samchil-Geun, Angelica, Ogapi, Phalanx, or safflower hot water.

Example  2: weight change measurement

Sprague-Dawely rats of 140-230g were purchased from Orient Bio Co., Ltd. (Seongnam, Korea) and supplied with sufficient feed and water for 1 week in experiment environment (temperature 22.2 ℃, humidity 55 ~ 66%). , And adjusted to 12 hours of contrast cycle.

Experimental group is four groups of seven per group by the ingot method so that the average weight is similar, that is, the normal control group (Control), diabetes control group (Streptozotocin (STZ)), diabetes induced the composition of the present invention prepared in Example 1 I & H was administered (STZ + I & H), and the composition I & H was administered (I & H) without causing diabetes, and then reared for 4 weeks.

Example  3: Streptozotocin  Diabetes caused by

Diabetes induction by streptozotocin was determined by Cho et al., Cho YJ, Bang MA, Hypoglycemic and Antioxidative Effects of Dietary Sea-Tangle Extracts Supplementation in Streptozotocin-Induced Diabetic Rats, The Korean Nutrition Society, 37 (1): 5-14, 2004).

Specifically, intraperitoneal injection of streptozotocin at 200 mg / kg body weight in 4-week-old male rats induced diabetes, and experimental breeding. After 4 weeks of experimental breeding, mice were fasted for 12 hours under ether anesthesia. The abdominal incision was made to collect whole blood with a syringe from the portal vein, and the serum was separated from the supernatant using a centrifuge. The spleen, liver, and lens of the eyeball were quenched with liquid nitrogen. It was used while storing at -70 ℃ until the experiment.

Example  4: blood glucose measurement

Glucose was measured using a blood glucose meter (Care SensⅡ, Inc., Korea), and the blood glucose content (mg / dl) was measured after blood collection in the vein.

Example  5: inflammation control, immunomodulation and Retinopathy  Adjustable measurement

TNF-α and IFN-γ, STAT1, pSTAT1, JAK1, T-bet, IL-12p35, IL-12p40, STAT4, IL-4, STAT6, pSTAT6, GATA3, IL-6 using Western Blotting , STAT3, pSTAT3 expression level was measured.

Specifically, the isolated spleen and liver were placed in 500 μl of lysis buffer (150 mM NaCl, 1% NP40, 0.5% deoxycholic acid, 0.1% sodium Dodecyl salfate, 50 mM Tris, 1tablet / 20mL protease inhibitor) and left on ice for 10 minutes. It was. This was centrifuged at 12,000 rpm for 20 minutes to obtain the protein dissolved in the buffer solution and the protein was quantified using a BSA protein assay kit. Each protein is mixed with 8%, 10%, 12% SDS sample buffer (4% SDS, 20% glycerol, 0.2% bromophenol blue, 2.8% 2-mercaptoethanol, 125 mM Tris-HCl pH6.8). Proteins were isolated by electrophoresis on 15% SDS-PAGE gel. The gel was electrically transferred to a nitrocellulose membrane and then used for immuno blot. To prevent non-specific reactions on nitrocellulose, membranes were treated with TBS-T (0.5M NaCl, 20 mM) containing 5% skim milk powder.

Block for 60 minutes with Tris-HCl pH7.2 0.2% tween20), wash with TBS-T (0.5M NaCl, 20 mM Tris-HCl pH7.2, 0.2% tween20) 6 times for 5 minutes, and then the primary antibody. Was diluted overnight in TBS-T containing 1 mg / mL BSA at a concentration of 1 μg / ml. The membrane was then washed 6 times with TBS-T for 5 minutes, and the secondary antibody was diluted in TBS-T containing 2% skim milk powder at a ratio of 1: 1000 and reacted for 1 hour, followed by 6 times for 5 minutes with TBS-T. Washed. The ECL solution was mixed with 1: 1 to emit light from the membrane, and then the amount of protein expression was measured.

In order to confirm cataract expression and retinopathy in diabetic rats and to test their inhibitory effects, the lens of the eye also used IL-6, STAT3, iNOS, through Western Blot analysis. The expression level of TNF-α was measured.

Claims (5)

A composition for preventing or treating diabetes or diabetic complications containing one or more selected from the group consisting of Samchil-Geun extract, Angelica extract, Ogapi extract, Phalanx extract and safflower seed extract. The composition for preventing or treating diabetes or diabetic complications of claim 1, wherein the extract is one or more selected from the group consisting of water, alcohols having 1 to 5 carbon atoms, and mixtures thereof. The method according to claim 1, wherein the one or more selected from the group consisting of Samchil-Geun Extract, Angelica Extract, Ogapi Extract, Phalanx Extract and Safflower Seed Extract are obtained by fractionation with one or more solvents selected from the group consisting of hexane, methylene chloride, ethyl acetate and butanol A composition for the prevention or treatment of diabetes mellitus or diabetic complications, containing fractions. According to claim 1, wherein the diabetic complications are diabetic retinopathy, diabetic cataract, diabetic keratosis, diabetic nephropathy, diabetic neuropathy, diabetic peripheral neuropathy, atherosclerosis and diabetic vascular complications Any one selected, a composition for preventing or treating diabetes or diabetic complications. A health food composition for preventing or improving diabetes or diabetic complications, comprising the composition according to any one of claims 1 to 4.

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