KR102172371B1 - A pharmaceutical composition comprising extract of Aloe, quince and corn silk for preventing or treating obesity - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating obesity, comprising an extract of presbyopia, quince, and okchok calligraphy as an active ingredient. The extract is significantly superior in the effect of inhibiting fat accumulation compared to the single extracts of nohoe, quince and okchok calligraphy, and has excellent effect of reducing weight, reducing body fat, and reducing blood triglyceride in an animal model in which obesity is induced. It can be usefully used as a pharmaceutical composition.

Description

{A pharmaceutical composition comprising extract of Aloe, quince and corn silk for preventing or treating obesity} comprising extracts of presbytery, quince and corn silk as active ingredients

The present invention relates to a pharmaceutical composition for preventing or treating obesity, comprising an extract of presbyopia, quince, and okchok calligraphy as an active ingredient.

With the recent rapid industrialization, improvement of income level, and rapid westernization of lifestyle patterns such as eating habits and diet, the number of patients with various adult diseases, such as cancer, diabetes, cardiovascular disease, and obesity, is rapidly increasing.

In general, obesity refers to a state in which fat tissue is abnormally increased by being converted into triglycerides and accumulated in adipocytes of the abdominal and subcutaneous fat tissue. Obesity itself is a disease that interferes with daily life, but there is a more serious problem that obesity causes various complications rather than the meaning of the disease itself.

Obesity causes hyperlipidemia, which increases the amount of cholesterol or triglycerides in the blood, and can lead to fatal diseases such as high blood pressure, cardiovascular disease and stroke, and peripheral tissues (Kelley, DE et al., Diabetes, 49(5)). , 677-683, 2000) and triglyceride accumulation in abdominal adipose tissue (Kelley, DE et al., Am J Physiol Endocrinol Metab., 278(5), E941-948, 2000). It can lead to type 2 diabetes, so long-term management and treatment is absolutely necessary. In addition, in the case of Asians, compared to Westerners, the body mass index is at least severe in abdominal obesity, and since they are highly susceptible to complications caused by arterial-related diseases such as hypertension, diabetes, and hyperlipidemia, obesity management is more important.

Diet to treat obesity, exercise therapy, behavior modification therapy to correct wrong eating and lifestyle habits, surgical therapy to reduce the volume of the intestine or stomach in surgery, appetite suppressants, diuretics, diuretics, or fiber to give a feeling of satiety Various methods, such as drug therapy using, are provided.

As for obesity treatment products developed to date, appetite suppressants, heat metabolism accelerators, digestive inhibitors, hormone regulators, etc. are known. Depending on the mechanism of action, the appetite suppressant classes include central adrenergic receptor stimulants (e.g. benzphetamine, phentermine), serotonin reuptake inhibitors (e.g. fenfluramine), adrenaline and serotonin reuptake inhibitors (e.g. Yes, it is divided into sibutramine). Heat metabolism accelerators (e.g. ephedrine/caffeine combination) increase the efficacy of adrenergic β3 receptors to increase the body's fever and energy consumption, thereby suppressing obesity. Appears outstanding. However, due to the nature of the mechanism of action of reducing appetite by acting on the central nervous system, there is a problem of causing serious side effects such as heart disease, increased blood pressure, increased heart attack, increased depression and suicide, while the lasting effect is inferior when used for a long time.

On the other hand, orlistat, a lipolysis inhibitor-based obesity treatment drug, reduces the absorption of triglycerides and cholesterol by irreversibly binding to and inactivating lipase, a lipolytic enzyme of the pancreas that degrades triglycerides. At the same time, it acted as an anti-obesity mechanism as a mechanism to promote excretion. However, Orlistat also has side effects such as abdominal pain, diarrhea, and inhibition of absorption of fat-soluble vitamins, and it is known to cause serious liver damage when taken for a long time, and safety review is currently underway.

Therefore, since most of the synthetic anti-obesity drugs on the market are known to have serious side effects, there is an urgent need to develop an obesity treatment agent that can effectively treat obesity without any side effects.

Aloe is a perennial herb belonging to the Liliaceae family, also known as aloe. Presbytery is known to be used for dry constipation due to heat and to remove parasites and pests because of its cold and cold properties. In addition, the presbytery has the effect of curing gastrointestinal diseases and asthma, and has the effect of healing cuts, burns, and sprained wounds, so it has been used not only for internal use but also for external use. As a compound isolated from the presbytery, chromone-based compounds such as aloesin and aloeresin are known.

Quince is the fruit of a deciduous tree belonging to the Rosaceae family. It is rich in sugar, calcium, phosphorus, iron, potassium, β-carotene and vitamin C, and contains about 2% saponin, 2~3% organic acids, flavonoids and tannins. In addition, it contains various nutrients.

In addition, quince has long been used for edible and medicinal purposes, and is known to be particularly effective in relieving cough, cold, and bronchitis. However, compared to general fruits, most of the quinces are discarded due to the fact that the moisture content is low, the astringency is strong, and the flesh is rough due to the development of stone cells and wood. Therefore, there is a need to recycle waste resources by evaluating the new functional value of quince and grafting it into various industries.

Corn silk is a yellow or light brown thread-like part that surrounds the fruit part of corn, and is also called corn beard, jade bal or jade mibal. In addition to fatty oils, essential oils, plant rubber substances, fats, bitter glycosides, resins, saponins, alkaloids, and maysin, inositol, cryptoxanthin, vitamin C , Vitamin K, sitosterol, stigmasterol, malic acid, tartaric acid, glucose, xylan, galactan, catechol, etc., and especially vitamin K is contained in large amounts.

The pharmacological action revealed so far has been reported to suppress radiation-induced oxidative stress, suppress the production of final glycosylated products related to complications of diabetes and aging, suppress diabetic nephropathy, antioxidant, and diuretic effects. It is also used as a food, flavoring, and food additive, and is listed as a GRAS (Generally Recognized As Safe) substance by FEMA (Flavor and Extract Manufacturers Association) and FDA (Food and Drug Administration). It is known to be used in baking.

On the other hand, as a prior art related to a composition for treating obesity comprising an extract of presbytery, quince and oyster calligraphy, Korean Patent No. 10-0510239 discloses the anti-obesity effect of a mixture containing corn beard and aloe, and Korean registered patent No. 10-1851639 discloses the anti-obesity effect of the Chinese quince extract, and Korean Patent No. 10-1732146 discloses the anti-obesity effect of the corn beard and tanja complex extract, and previous papers [Pothuraju, R. et al., Phytother Res., 30(2), 200-207, 2016] discloses the anti-obesity effect of the aloe extract, but mentions the anti-obesity effect of the mixed extract including all of the presbytery, quince and oyster calligraphy as in the present invention. Nothing happened.

In the course of researching a composition for anti-obesity, the present inventors were able to complete the present invention by confirming that the extract obtained by mixing presbytery, quince and oyster calligraphy at a specific mixing ratio has particularly excellent anti-obesity effect compared to the single extract.

Korean Patent Registration No. 10-0510239, Method for producing a composition containing corn whiskers and dietary fiber, registered on August 18, 2005. Korean Patent Registration No. 10-1851639, Anti-obesity composition containing quince extract or fractions thereof as an active ingredient, registered on April 18, 2018. Korean Patent Registration No. 10-1732146, Anti-obesity composition containing corn beard and tanja complex extract, registered on April 25, 2017.

Kelley, D. E. et al., Fuel selection in human skeletal muscle in insulin resistance: a reexamination, Diabetes, 49(5), 677-683, 2000. Kelley, D. E. et al., Subdivisions of subcutaneous abdominal adipose tissue and insulin resistance, Am J Physiol Endocrinol Metab., 278(5), E941-948, 2000. Pothuraju, R. et al., Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review, Phytother Res., 30(2), 200-207, 2016.

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating obesity comprising an extract of presbytery, quince and oyster calligraphy as an active ingredient.

The present invention relates to a pharmaceutical composition for preventing or treating obesity, comprising an extract of presbyopia, quince, and okchok calligraphy as an active ingredient.

The presbytery, quince, and okchok calligraphy examples are extracted after mixing in a weight ratio of 1 to 3: 0.5 to 2: 2.5 to 5, and the presbytery extract, quince extract, and okchok calligraphy extract of 1 to 3: 0.5 to 2: 2.5 to 5 It is also possible to mix by weight ratio. However, it is not preferable because the anti-obesity activity is remarkably lowered in the case of mixing noshoe, quince, and okchok calligraphy outside the mixing weight ratio condition.

The extract is a mixture of presbyopia, quince and oyster calligraphy, or each presbytery, quince and oyster calligraphy extracted using water, C1 to C4 alcohol or a mixed solution thereof as a solvent, and the alcohols of C1 to C4 are methanol, ethanol, and propanol. , It may be selected from the group consisting of isopropanol, butanol and isobutanol. Water used for extraction, C1 to C4 alcohol, or a mixed solution thereof may be used alone or in a volume of 2 to 50 times the weight of a mixture of presbyopia, quince, and oyster calligraphy used, and the above process may be repeated 1 to 5 times. Do.

The extraction time of the extract is not particularly limited, but it is preferable to extract within 10 minutes to 1 day, and the extraction method includes all commonly used extraction methods, such as pressure extraction, immersion (cold immersion, warm needle), ultrasonic extraction, A reflux extraction method, etc. may be used, and a conventional extraction device, an ultrasonic grinding extractor, or a fractionator may be used as an extraction device.

The extract may further include filtering to remove suspended solid particles. Cotton, nylon, or the like may be used to filter particles, or ultrafiltration, freeze filtration, centrifugation, etc. may be used, and a method in which a known method used for separation and extraction of plants may be used alone or in an appropriate combination.

In addition, the extract is formed using the extract itself and the extract, such as an extract obtained from the extraction process, a dilute or concentrate of the extract, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof. Includes extracts of all possible formulations.

The pharmaceutical composition according to the present invention may be formulated in a suitable form together with a generally used pharmaceutically acceptable carrier. "Pharmaceutically acceptable" refers to a composition that is physiologically acceptable and, when administered to a human, does not usually cause an allergic reaction or similar reaction such as gastrointestinal disorders, dizziness, and the like. In addition, the compositions may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method.

Carriers, excipients, and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum arabic, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl Cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl paraoxybenzoate, propyl paraoxybenzoate, talc, magnesium stearate, and mineral oil may be included, but are not limited thereto. In the case of formulation, it is prepared using diluents or excipients such as commonly used fillers, stabilizers, binders, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient, for example, starch, microcrystalline cellulose, sucrose or lactose, in the extract of the present invention, It is prepared by mixing low-substituted hydroxypropyl cellulose and hypromellose. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, and preservatives may be included. . Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used as the non-aqueous solvent and the suspension. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin, glycerol, gelatin, and the like may be used. In order to formulate a formulation for parenteral administration, the extract or a pharmaceutically acceptable salt thereof is sterilized and/or an adjuvant such as a preservative, a stabilizer, a hydrating agent or an emulsifying agent, a salt and/or a buffer for controlling the osmotic pressure, and other treatments It can be prepared as a solution or suspension by mixing in water with a useful substance, and it can be prepared in ampoules or vials unit dosage form.

The pharmaceutical composition comprising the extract disclosed in the present invention as an active ingredient can be administered to mammals such as mice, livestock, and humans by various routes. All modes of administration can be expected and can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injection. The dosage is the age, sex, weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the time of administration, the route of administration, the absorption, distribution and excretion rate of the drug, the types of other drugs used, and the prescriber's It will depend on your judgment, etc. Dosage determination based on these factors is within the level of one of ordinary skill in the art, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is from 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be divided several times. The above dosage does not in any way limit the scope of the present invention.

In yet another aspect, the present invention relates to a health functional food for preventing or improving obesity, comprising an extract of presbytery, quince and okchok calligraphy as an active ingredient. The health functional food refers to a food manufactured or processed using raw materials or ingredients having useful functionality, and includes, for example, health supplements, functional foods, nutritional supplements, and supplements.

The extract may be added in an amount of preferably 0.001% to 50% by weight, more preferably 0.001% to 30% by weight, and most preferably 0.001% to 10% by weight based on the total weight of the health functional food. have.

The health functional food of the present invention includes the form of tablets, capsules, pills or liquids, and foods to which the extract of the present invention can be added include, for example, various foods, beverages, gums, teas, vitamin complexes, etc. There is this.

The present invention relates to a pharmaceutical composition for preventing or treating obesity, comprising an extract of presbyopia, quince, and okchok calligraphy as an active ingredient. The extract is significantly superior in the effect of inhibiting fat accumulation compared to the single extracts of nohoe, quince and okchok calligraphy, and has excellent effect of reducing weight, reducing body fat, and reducing blood triglyceride in an animal model in which obesity is induced. It can be usefully used as a pharmaceutical composition.

1 is a result of confirming the change in fat cell accumulation according to the treatment of Examples and Comparative Examples of the present invention in 3T3-L1 cells by Oil red O staining.
2 is a result of confirming the change in triglyceride mass according to the treatment of Examples and Comparative Examples of the present invention in HepG2 cells.
3 is a result of confirming the change in the amount of visceral fat and abdominal fat according to the treatment of Examples and Comparative Examples of the present invention in an obesity-induced animal model.
4 is a result of confirming the change in the amount of triglyceride in blood according to the treatment of Examples and Comparative Examples of the present invention in an animal model in which obesity is induced.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the content introduced herein becomes thorough and complete, and is provided to sufficiently convey the spirit of the present invention to those skilled in the art.

<Example 1. Preparation of extracts containing presbytery, quince and oyster calligraphy>

200 g of nohoe (Gyeongdong market), 100 g of Chinese quince (Gyeongdong market), and 350 g of Okchok calligraphy (Gyeongdong market) were mixed, and then 13ℓ of 95% ethanol aqueous solution was added, followed by stirring and extraction at 20°C at 200 rpm for 72 hours. The extract obtained from the above process was filtered to obtain a filtrate, which was concentrated under reduced pressure at 40° C. with a rotary vacuum concentrator, and then freeze-dried to obtain about 93 g (14.3% yield) of powdery extracts of Noshoe, Chinese quince and Okchok Calligraphy. The freeze-dried powder obtained from the above process was stored at -80°C until use.

<Comparative Example 1. Preparation of noshoe extract>

In the same manner as in Example 1, but instead of mixing 200 g of noshoe, 100 g of quince, and 350 g of Okcheok calligraphy, 650 g of noshoe was used alone to obtain a comparison target noshoe extract.

<Comparative Example 2. Preparation of quince extract>

It was prepared in the same manner as in Example 1, but instead of mixing 200 g of noshoe, 100 g of quince, and 350 g of quince, 650 g of quince was used alone to obtain a quince extract for comparison.

<Comparative Example 3. Preparation of extract of Okchok calligraphy>

It was prepared in the same manner as in Example 1, but instead of mixing 200 g of noshoe, 100 g of quince, and 350 g of Okchok calligraphy, 650g of Okchok calligraphy was used alone to obtain an extract of Okchok calligraphy for comparison.

<Experimental Example 1. Confirmation of anti-obesity effect in adipocytes>

Experimental Example 1-1. Confirmation of fat accumulation inhibitory effect

In the adipocyte 3T3-L1 cells, the effect of inhibiting fat accumulation according to the treatment of the extracts of Presbyopia, Chinese quince and Okchokseoye of Example 1 of the present invention was confirmed by Oil red O staining.

3T3-L1 cells (Korean cell line bank, KCLB) are DMEM (Dulbecco's modified eagle medium) in a medium containing 10% FCS (Fetal calf serum) and 1% Penicillin-Streptomycin (Penicillin-Streptomycin). And cultured in an incubator under conditions of 37° C. and 5% CO ₂ .

For differentiation into adipocytes, 3T3-L1 cells were dispensed into a 6-well plate at 5×10 ⁵ cells/well, and cultured while changing the medium once every two days until the cells became 100% confluent. DMEM medium containing 0.5mM IBMX (3-isobutyl-1-methylxanthine), 1 μM dexamethasone, 1 μM/mL insulin and 10% Fetal bovine serum (FBS) 2 days after the 100% confluent state was confirmed Adipocyte differentiation was initiated by culturing at for 2 days.

In order to induce differentiation, an extract of Example or Comparative Example dissolved in a concentration of 100 µg/ml in 50% ethanol aqueous solution when differentiation was completed with the naked eye when the differentiation was completed with DMEM medium containing 1µg/ml insulin and 10% FBS. Was treated on the cells and cultured for 48 hours.

After 48 hours, the culture medium of 3T3-L1 cells was removed, washed with phosphate buffered saline, and then the cells were fixed in an incubator at 37° C. for 1 hour using 10% formalin solution. Thereafter, the formalin solution was removed, washed with primary distilled water, and then oil red O dyeing solution was added and dyed at room temperature for 2 hours. After dyeing, washing with distilled water and dissolving in 60% isopropanol, the absorbance was analyzed at a wavelength of 500 nm, and the fat globule accumulation rate was calculated from the absorbance analysis result and shown in FIG. 1.

Referring to FIG. 1, the extracts (100 µg/ml treatment) of Nohoe, Chinese quince and Okchok-seoye of Example 1 of the present invention were applied to the adipocytes with a single extract (100µg/ml treatment) as in Comparative Examples 1 to 3. In comparison, the effect of inhibiting fat cell accumulation was excellent.

Particularly, the present inventors mixed and extracted presbytery, quince, and okchok calligraphy at various mixing ratios before carrying out the above experiment, and confirmed the fat globule accumulation rate.As a result, unlike in Example 1, at least one of presbytery, quince and okchok calligraphy If the extract does not contain any ingredients or the mixing ratio of presbytery, quince and quince is out of 1~3:0.5~2:2.5~5 and contains more than 4 times more or less presbytery and quince based on quince It was confirmed that the synergistic effect by was low.

From this, it can be seen that the use of a mixture of presbytery, quince and oyster calligraphy at a specific mixing ratio as in the present invention is the most effective in showing the anti-obesity effect, rather than using the single extract of presbytery, quince and quince calligraphy when obesity prevention or treatment. there was.

Experimental Example 1-2. Checking the effect of inhibiting the accumulation of triglycerides

In order to confirm the effect of inhibiting the accumulation of triglycerides according to the treatment of the present invention presbytery, Chinese quince, and Okchokseoye extract, the amount of triglyceride in HepG2 cells was measured.

First, HepG2 cells were aliquoted into 4×10 ⁵ cells/well, cultured for 24 hours, and then treated with 2mM palmitic acid and cultured for additional 24 hours. Thereafter, it was exchanged with a medium containing Examples or Comparative Examples at a concentration of 100 μg/ml and cultured for 24 hours. After incubation, the culture medium was removed, the cells were collected, and the cells were lysed with 5% Triton X-100. The cell lysate was centrifuged to obtain a supernatant, and then the amount of triglyceride was quantified using a triglyceride measurement kit (Sigma-aldrich, MO, USA), and the results are shown in FIG. 2.

Referring to FIG. 2, it was found that the amount of triglycerides was reduced by two or more times compared to treatment with a single extract as in Comparative Examples 1 to 3 in the Nohoe, Chinese quince, and Okchokseoye extract of Example 1 of the present invention.

Therefore, from the above results, it was found that the extracts of the present invention presbytery, quince and oyster seeds have excellent effects of inhibiting fat accumulation and suppressing triglycerides, and thus can be usefully used as a composition for preventing or treating obesity.

< Experimental example 2. In animal models Anti-obesity Check the effect>

Experimental Example 2-1. Check changes in weight, visceral fat and abdominal fat in obese animals

To build obese animals, 5-week-old male C57BL/6J mice (Orient Bio, Korea) work in an animal testing facility that maintains a temperature of 22~25℃, a relative humidity of 50~60%, a light and dark cycle of 12 hours, and an illuminance of 200~300 LUX. Purified for a week. Two animals were reared in polycarbonate boxes for mice, and normal feed (Purina Co., Korea) and drinking water were freely fed for one week during the acclimatization period.

After acclimatization, 16 animals were separated per group so that the average weight was similar, and the normal group was subjected to a general diet (10% kcal fat diet, Research Diets Inc. NJ, USA), and the control group, Example 1, and Comparative Example 3 were fed a high fat diet. (60% kcal fat diet (Research Diets Inc. NJ, USA) was freely fed for 8 weeks.

In the normal group, 0.5% CMC (carboxymethylcellulose) solution was administered orally once a day for 56 days for a total of 56 times from the day of ingestion, and the control group received 0.5% CMC solution once daily for 56 days from the day of ingestion of the high fat diet. A total of 56 times were administered orally, and in Example 1 or Comparative Example 3, the extract of Example 1 or Comparative Example 3 was prepared daily in a 0.5% CMC solution from the day of ingesting a high fat diet so that the concentration of each of the extracts was 500 mg/kg. A total of 56 times were administered orally once daily for 56 days.

The weight of obese animals was measured twice a week, and the weight of visceral fat and abdominal fat was measured after fasting for 15 hours from the evening after administration on the last day of the experiment, and the animals were sacrificed to separate visceral fat and abdominal fat, and their weights were measured. Changes in weight of obese animals are shown in Table 1, and changes in visceral fat and abdominal fat are shown in FIG. 3.

Condition weight Weight gain (g) Start of test (g) End of test (g) Normal Regular diet +0.5% CMC 20.4±0.2 25.9±0.3 5.5±0.3 Control High fat diet +0.5% CMC 20.3±0.2 36.0±1.1 ^** 15.7±1.0 ^** Example 1 High fat diet +0.5% CMC
+500 mg/kg Example 1 20.3±0.2 31.3±0.9 ^## 11.0±0.8 ^## Comparative Example 3 High fat diet +0.5% CMC
+500mg/kg Comparative Example 3 20.3±0.2 33.5±0.8 ^# 13.2±0.7 ^{# **} p <0.01 vs normal group; ^# p <0.05, ^## p <0.01 vs control

Looking at Tables 1 and 3, when administering Example 1 of the present invention to an animal model in which obesity was induced, the amount of body weight, visceral fat, and abdominal fat was significantly reduced compared to the case of administering the control or Comparative Example 3.

From these results, it can be seen that the presbytery, quince, and okchok calligraphy extracts mixed at a specific mixing ratio as in the present invention have a much better anti-obesity effect than the single administration of the extract of Okchokseoye, which was the most effective among individual herbal medicines. there was.

Experimental Example 2-2. Confirmation of changes in blood triglycerides in obese animals

As in Experimental Example 2-1, the normal group, the control group, Example 1 or Comparative Example 3 were orally administered once a day for 56 days for 56 days for a total of 56 times, and after fasting for 15 hours from the evening of the last test substance administration, 0.4 ml/ Corn oil was orally administered as a head dose. Before corn oil administration (0 hour), 2, 4, 6 hours after administration, blood was collected from the tail of an animal model, and triglycerides were measured with a triglyceride meter (Accutrend plus, Roche, Switzerland), and this is shown in FIG. 4.

Referring to Figure 4, when administering the presbytery, Chinese quince, and Okchokseoye extract of Example 1 of the present invention to an animal model in which obesity is induced, the amount of triglyceride in blood was significantly greater than 100 mg/dL compared to the case of administering the control group. It was confirmed that it decreased. However, in the case of administering the extract of Okchokseo Example of Comparative Example 3 to an animal model in which obesity was induced, unlike Example 1 of the present invention, there was no significant blood triglyceride reduction effect compared to the control group.

Therefore, it was found that the use of presbyopia, quince, and okchok calligraphy at a specific mixing ratio, as in the present invention, has the most excellent effect on treating obesity.

<Formulation Example 1. Preparation of tablets>

20 g of the extracts of Noshoe, Chinese quince and Okchok calligraphy of Example 1 of the present invention were mixed with 175.9 g of lactose, 180 g of potato starch, and 32 g of colloidal silicic acid, respectively. After adding a 10% gelatin solution to this mixture, it was pulverized and passed through a 14 mesh sieve. This was dried, and 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate were added thereto, and the resulting mixture was made into tablets.

<Formulation Example 2. Preparation of capsules>

After mixing 100 mg of presbyopia, quince and oyster calligraphy extract of Example 1 of the present invention, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate, the above ingredients were mixed according to a conventional capsule preparation method, and then in a gelatin capsule. Filled to prepare a capsule.

<Formulation Example 3. Preparation of injection>

1 g of the extract of Noshoe, Chinese quince, and Okchok calligraphy of Example 1, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water to make 100 ml. This solution was put in a bottle and sterilized by heating at 20° C. for 30 minutes.

<Formulation Example 4. Preparation of health functional food>

Presbytery of the present invention Example 1, quince and oyster extract 20g, vitamin mixture appropriate amount, vitamin A acetate 70 ㎍, vitamin E 1.0 mg, vitamin B1 0.13 mg, vitamin B2 0.15 mg, vitamin B6 0.5 mg, vitamin B12 0.2 ㎍, Vitamin C 10 mg, biotin 10 ㎍, nicotinic acid amide 1.7 mg, folic acid 50 ㎍, pantothenate calcium 0.5 mg, an appropriate amount of mineral mixture, ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium monophosphate 15 mg , Dibasic calcium phosphate 55mg, potassium citrate 90mg, calcium carbonate 100mg, magnesium chloride 24.8mg were mixed to form granules, but can be prepared by modifying various formulations according to the use. In addition, the composition ratio of the vitamin and mineral mixture may be arbitrarily modified, and may be prepared by mixing the above ingredients according to a conventional health functional food manufacturing method.

< Formulation example 5. Manufacturing of health functional beverages>

A beverage was prepared by mixing 1 g of an extract of Noshoe, Chinese quince and oyster Calligraphy Example 1 of the present invention, 0.1 g of citric acid, 100 g of fructooligosaccharide, and 900 g of purified water, followed by stirring, heating, filtering, sterilization, and refrigeration according to a conventional beverage preparation method.

Claims (6)

A pharmaceutical composition for preventing or treating obesity, characterized in that a mixture of presbytery, quince, and oyster calligraphy in a weight ratio of 1 to 3: 0.5 to 2: 2.5 to 5 is extracted with ethanol or an aqueous ethanol solution. delete delete A health functional food for preventing or improving obesity, characterized in that a mixture of presbytery, quince and quince, and a mixture in a weight ratio of 1 to 3: 0.5 to 2: 2.5 to 5 is extracted with ethanol or an aqueous ethanol solution. delete delete

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