KR20150113434A - A composition comprising the extract of ginseng seed for protecting brain cells and preventing, improving and treating depression - Google Patents

Abstract

Provided is a novel use of an extract of ginseng seeds for protecting brain cells and preventing, treating, or relieving depression. A composition containing the extract of ginseng seeds as an active ingredient shows neuron protecting effects against toxicity due to corticosterone which is associated with depression inducement, and improvement effects on the expression of neuronotrophic factors, thereby being effectively used for preventing or relieving the development and progression of depression, or treating depression.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing, improving and treating cranial nerve cell protection and depression including ginseng seed extract,

The present invention relates to a new use of ginseng seed extract for the prevention of neuronal cell protection and prevention, improvement and treatment of depression.

Depression is also referred to as a "cold of the mind" and is the highest suicide rate among all human illnesses. It is known that a person has a higher morbidity rate than any mental illness, have.

The cause of depression has not been clarified. Recently, various molecular mechanisms have been found to cause the depression. It is known that depression is triggered by various mechanisms, not by a single cause.

First, depression is inherited by genetic or biological markers, which are known to be caused by stress. Stress increases the expression of corticotropin releasing factor (CRF) mRNA in the paraventricular nucleus (PVN) of the hypothalamus, increases glucocorticoid receptor expression in amygdala, and many of these patients have abnormal HPA axis. Term depression.

Next, recent studies on stress and depression are centered on the hippocampus. After stress, hippocampal atrophy or pyramidal neuron in hippocampal CA3 region is decreased. Brain imaging studies have also shown that hippocampus volume is reduced in patients with depression or posttraumatic stress disorder. The atrophy of the hippocampus and loss of neurons were reported to be related to the psychopathology of depression or posttraumatic stress disorder.

In addition, the anticipation that antidepressants will reverse the stress-induced morphological changes of hippocampal neurons or the loss of neurons is amplified as they are known to promote brain-derived neurotrophic factor (BDNF) expression in the hippocampus It is a situation. BDNF is known to be involved mainly in neuronal differentiation and survival in the developmental stage of the brain. Actual antidepressant long term treatment has been shown to restore the atrophy of stress-induced nerve cell processes.

Currently, depression-related treatments are mainly psychotherapy such as cognitive-behavioral therapy (CBT), interpersonal therapy (IPT), and counseling, electroconvulsive therapy using electrical stimulation, drug therapy using antidepressants and nystagmus The main purpose of the treatment for depression is to improve the symptoms by activating the actions of 'serotonin' or 'noradrenaline', which are mainly responsible for information transmission between brain cells.

These agents are developed and marketed for each purpose. They include SSRIs (selective serotonin reuptake inhibitors), paroxetine, celtralin, and SNRI (serotonin and noradrenaline), which are used to inhibit serotonin or noradrenaline reabsorption Milaseprin, which is an absorption-suppressing drug, and three generations of generic drugs, aminotropicrin, and amosafin, which are four-generation generic drugs.

However, these preparations have excellent therapeutic effect, but due to the nature of the disease, they require a prescription and require long-term use, and there are side effects such as vomiting due to long-term use, digestive problems, enlargement of the prostate gland and induction of female diseases due to menstrual irregularities.

Therefore, there is a growing need for a preventive or therapeutic agent and a preventive or remedy food containing an effective amount of a natural substance having a low side effect.

On the other hand, ginseng seeds are mainly used in relation to ginseng cultivation and breeding, so the analysis and research results of the ingredients and the commercialization thereof are insufficient. Ginseng (Panax ginseng CA Meyer) is a herb that has been used in Korea, China and Japan for over 2,000 years. It has been used for the purpose of preventing diseases and extending life span, , Anti-cancer, immune function enhancement, anti-diabetic, liver function is known to be effective. However, since the ginseng seed was used only as a means for cultivating ginseng, studies on the oil contained in ginseng seeds, especially ginseng seed, were conducted by analyzing the fatty acid composition and phytosterol content of ginseng seed oil (Non-Patent Document 1) Only the content of polyphenol component is studied.

 J. Agric. Food Chem. 50, 744-750, 2002

The present invention aims at finding components derived from natural materials which can replace conventional drugs that have been used for the prevention, improvement and treatment of neuronal cell protection and depression.

The inventors of the present invention have conducted research on natural products for depression, a representative disease of stress diseases, and have found that the extract of ginseng seed having low industrial value exhibits neuronal cell protective activity and has an effect of preventing, improving or treating depression And completed the present invention.

The present invention relates to a novel use of ginseng seed extract for the prevention and improvement and treatment of neuronal cell protection and depression, a pharmaceutical composition for prevention, improvement and treatment of neuronal cell protection and depression comprising ginseng seed extract as an active ingredient, Comprising administering to a subject an effective amount of a ginseng seed extract. Prevention, improvement, and treatment.

In the following examples, ginseng seed extract was treated with PC12 cell, which is a neuron, to inhibit neuronal cell damage to corticosterone. In addition, neuronal cell differentiation and regeneration inhibited by corticosterone- Induced neurotrophic factor (BDNF) and cyclic adenosine monophosphate response element binding protein (CREB). Therefore, the ginseng seed extract can be used as an active ingredient of a composition for prevention, improvement and treatment of neuronal cell protection and depression.

In the present invention, depression refers to a depressive disorder caused by chronic stress, which is a major symptom of desensitization and depression, and causes a variety of cognitive and psychosomatic symptoms, resulting in impaired function of daily life .

In the present invention, the ginseng seed extract includes all the extracts obtained by the pressing method or the solvent extraction method.

In one embodiment of the present invention, the ginseng seed extract may be extracted from ginseng seed using water, an organic solvent, or a mixture thereof as an extraction solvent. The kind of the organic solvent used at this time and the mixing ratio of water and the organic solvent are not particularly limited.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, as the lower alcohol, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol can be used. The organic solvent may be a polar solvent such as acetic acid, dimethyl-formamide (DMFO) or dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, decane 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1- chloropropane, chlorobenzene , Non-polar solvents such as benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (tetrahydrofuran) may be used.

In one embodiment, the ginseng seed extract may be a hot-water extract or a lower alcohol extract of ginseng seed. Preferably, it may be an ethanol extract of ginseng seeds. In another embodiment, the ginseng seed extract may be a hexane extract of ginseng seed. In the present specification, the extract of organic solvent includes organic solvent alone or extract obtained by using a mixed solvent of organic solvent and water as extraction solvent. For example, the ethanol extract of Sasao includes not only extracts using ethanol alone but also extracts obtained by using a mixed solvent of water and ethanol.

For example, the ginseng seed extract of the present invention may be added to dried ginseng seeds in an amount of 10 to 20 times, preferably 15 times (w / v) of water, a lower alcohol such as ethanol, methanol or butanol, Preferably 70 to 120 ° C, preferably 80 to 110 ° C, in a solvent, preferably water or 5 to 95% ethanol by a conventional extraction method such as a heat extraction method, an ultrasonic extraction method or a reflux extraction method, For a period of time, preferably 3 to 5 hours; A second step of filtrating and concentrating the extract obtained in the above step under reduced pressure; And then lyophilized at -50 캜 to -30 캜, preferably at -45 캜 to -25 캜 for 24 to 72 hours, preferably for 40 to 55 hours. Seed extract can be obtained.

As used herein, the term " extract " also includes fractions obtained by further fractionating the extract. That is, the ginseng seed extract is obtained not only by using the above-mentioned extraction solvent but also by additionally applying a purification process thereto. Further, fractions obtained by passing the above extract or fractions through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), and the like The fractions obtained through various purification methods were also included in the ginseng seed extract of the present invention.

In one embodiment, the ginseng seed extract may be a fraction obtained by fractionating an organic solvent extract of ginseng seed with a second organic solvent. In another embodiment, the ginseng seed extract may be a fraction obtained by re-fractionating a lower alcohol extract of ginseng seed with a second organic solvent. For example, the ginseng seed extract may be a fraction obtained by fractionating an ethanol extract of ginseng seeds with hexane.

As used herein, the term " ginseng seed extract " includes ginseng seed extract as well as crude extract obtained by treating an extract solvent with ginseng seed. For example, ginseng seed extract may be prepared in powder form by further processes such as vacuum distillation and lyophilization or spray drying.

The composition of the present invention may contain the ginseng seed extract in an amount of 0.01 to 50% by weight based on the total weight of the composition. Such a composition is not limited thereto, and may vary depending on the concentration of the ginseng seed extract, the condition of the subject to which the composition of the present invention is administered, the type of disease, and the degree of progression.

As used herein, the term " comprising as an active ingredient " means an amount sufficient to achieve the efficacy or activity of ginseng seed extract. In one embodiment of the invention, the ginseng seed extract in the composition of the present invention is administered in an amount of, for example, 0.01 mg / ml or more, preferably 0.05 mg / ml or more, more preferably 0.1 mg / ml or more And more than 1 mg / ml. Since the ginseng seed extract has no adverse effect on the human body even when administered in an excessive amount as a natural product, the quantitative upper limit of the ginseng seed extract contained in the composition of the present invention can be selected by a person skilled in the art within a suitable range.

The composition according to the present invention can be utilized as a pharmaceutical composition or a food composition.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention can be administered orally or parenterally. In the case of parenteral administration, the composition can be administered by intravenous injection, subcutaneous injection, muscle injection, intraperitoneal injection, transdermal administration, etc., .

The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Usually, a skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.0001-10 g / kg.

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The present invention also provides a food composition for prevention and improvement of neuronal cell protection and depression comprising ginseng seed extract as an active ingredient.

The food composition according to the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectioneries, diet bars, dairy products, meat, chocolates, pizza, ram noodles, other noodles, gums, ice cream, .

The food composition of the present invention may contain not only ginseng seed extract as an active ingredient, but also components that are ordinarily added in the manufacture of food, including, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavors . Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, various plant extracts and the like may be further added in addition to the ginseng seed extract of the present invention .

The present invention provides a health functional food comprising the ginseng seed extract as an active ingredient and a food composition for preventing and ameliorating neuronal cell protection and depression. Health functional foods are foods made by adding ginseng seed extract to food materials such as beverages, tea, spices, gum and confectionary, or encapsulated, powdered, or suspended, However, unlike general medicine, there is an advantage that there is no side effect that can occur when a food is used as a raw material and a drug is taken for a long time. The health functional food of the present invention thus obtained is very useful because it can be ingested routinely. The amount of the ginseng seed extract added to such a health functional food can not be uniformly determined depending on the type of the health functional food to which it is added but may be added within a range that does not impair the original taste of the food, 0.01 to 50% by weight, preferably 0.1 to 20% by weight. In the case of health functional foods in the form of pills, granules, tablets or capsules, they may be added usually in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

The present invention also provides the use of said ginseng seed extract for the manufacture of a medicament or food for the prevention, amelioration and treatment of neuronal cell protection and depression. As described above, the ginseng seed extract can be used for prevention, improvement and treatment of brain nerve cell protection and depression.

The present invention also provides a method for preventing, ameliorating and treating neuronal cell protection and depression, which comprises administering an effective amount of ginseng seed extract to a mammal.

As used herein, the term " mammal " refers to a mammal that is the subject of treatment, observation, or experimentation, preferably a human.

The term " effective amount " as used herein refers to the amount of active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as contemplated by a researcher, veterinarian, physician or other clinician, ≪ / RTI > inducing a reduction of the symptoms of the disease or disorder. It will be apparent to those skilled in the art that the effective amount and the administration frequency of the active ingredient of the present invention will vary depending on the desired effect. Thus, the optimal dosage to be administered can be readily determined by those skilled in the art and will vary with the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, The age, body weight, sex, diet, time of administration, route of administration and fraction of the composition, duration of treatment, concurrent medication, and the like. In the prevention, treatment, or improvement of the present invention, it is preferable that the ginseng seed extract is administered at a dose of 0.001 mg / kg to 10 g / kg once or several times a day.

The composition comprising the ginseng seed extract as an active ingredient in the treatment method of the present invention can be administered orally or rectally through the oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, ≪ / RTI >

The composition comprising the ginseng seed extract of the present invention as an active ingredient exhibits a neuroprotective effect on the toxicity caused by cortisol and a neurotrophic factor expression improving effect related to the induction of depression so that the occurrence and progress of depression can be prevented or improved, Can be effectively used to treat depression.

Figure 1 shows the effect of water and ethanol extracts of ginseng seeds on cytotoxicity (Control: untreated control group, 0: ginseng seed hydrothermal extract, 50: 50% EtOH ginseng seed extract, 80: 80% EtOH ginseng seed extract, Final concentrations of sample: 0.1 mg / ml and 0.5 mg / ml).
Figure 2 shows the protective activity of water and ethanol extract of ginseng seeds from corticosterone (Control: untreated control, control-1: corticosterone treatment (0.2 mM, 48 hr), 0: , 50: 50% EtOH ginseng seed extract, 80: 80% EtOH ginseng seed extract).
Figure 3 shows that the water and ethanol extracts of ginseng seed improve the inhibition of the expression of nerve cell nutrients caused by corticosterone (Control: untreated control, control-1: corticosterone treatment (0.2 mM, 48 hrs ), 0: ginseng hot water extract, 50: 50% EtOH ginseng seed extract, 80: 80% EtOH ginseng seed extract).

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

Example  1. Ginseng seed Heat number  Preparation of extract

The dried ginseng seed sample was ground to obtain 3.2 kg of the ground sample. 36 L of purified water was added and heated at 100 ° C. for 4 hours. The filtrate was concentrated under reduced pressure and freeze-dried at -40 ° C. for 48 hours using a freeze dryer, Ginseng seed hot water extract was used as a sample of the following experimental example.

Example  2. Preparation of Ethanol Extract of Ginseng Seed

2-1. Production of 50% Ethanol Extract of Ginseng Seed

The dried ginseng seed sample was crushed, and 36 L of 50% ethanol was added to the ground sample (3.2 kg), and the mixture was heated at 84 ° C. for 4 hours. The filtrate was concentrated under reduced pressure and freeze-dried at -40 ° C. for 48 hours Dried to obtain a 50% ethanol extract of ginseng seed of the present invention and used as a sample of the following experimental example.

2-2. Production of 80% Ethanol Extract of Ginseng Seed

The dried ginseng seeds were crushed, and 36 L of 95% ethanol was added to the ground sample (3.2 kg), and the mixture was heated at 82 ° C for 4 hours. The filtrate was concentrated under reduced pressure and freeze-dried at -40 ° C for 48 hours Dried to obtain a 95% ethanol extract of ginseng seed of the present invention and used as a sample of the following experimental example.

Experimental Example  1. Cytotoxicity experiment

In order to test the cytotoxicity of the samples obtained in the above examples, MTT assay method (Yun-Lian Lina, Guei-Jane Wang, Chuen-Lin Huan, Yi-Chao Lee, Wei-Chen Liao, Wen- , Nai-Kuei Huan, J. Ethnopharmacology, 122: 417-425 (2009)).

(Gibco-BRL, Gaithersburg, MD) and 1% antimycotics / antibiotics (Gibco-BRL) supplemented with 10% fetal bovine serum cultured in RPMI-1640 medium containing one (Gibco-BRL) the number of cells were cultured to be 10 ⁴ -10 ⁶ cells / ml.

The cultured cells were transferred to a 96-well plate, and the number of cells was cultured at 1 × 10 ⁴ cells / well. Then, 1 mg / ml and 5 mg / ml of the ginseng seed extract obtained in the above Example were dissolved in 5% DMSO, (Final concentrations of 0.1 mg / ml and 0.5 mg / ml) for 48 hours at 37 ° C and then reacted with MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, M2128 , Sigma) Reagent 2 mg / ml was added to each well at 15. After incubation at 37 캜 for 90 minutes, absorbance was measured at 570 nm / 630 nm and calculated using the following equation (1).

[Equation 1]

Cell survival rate (%) = (absorbance _(sample) / absorbance _{(control group)} ) 100

Figure 1 shows the effects of water and ethanol extracts of ginseng seeds on cytotoxicity (Control: no treatment, 0: ginseng seed hydrothermal extract, 50: 50% EtOH ginseng seed extract, 80: 80% EtOH ginseng seed extract, Final concentration of sample: 200 占 퐂 / ml). As shown in FIG. 2, it was confirmed that the ginseng seed extract samples were not cytotoxic.

Experimental Example  2. Corticosterone  Measurement of protective activity of neuron

To examine the protective activity of nerve cells from the toxicity of corticosterone in the samples obtained in the above examples, Xiao-Qing Tang, Jian-Qiang Feng, Jin Chen, Pei-Xi Chen, Jun- Li Zhi, Yu Cui, Rui-Xian Guo, Hui-Min Yu; Brain Research, 1057 57-64 (2005)).

PC12 cells were cultured in a 96-well plate in RPMI-1640 medium containing antimicotics / antibiotics. When the number of cells became 1 × 10 ^{4 per} well, the ginseng seed extract obtained in the above Example was dissolved in 5% DMSO , 15 μl of each of them was added to each well, pre-incubated at 37 ° C for 2-4 hours, treated with 0.2 mM corticosterone for 48 hours to induce oxidative stress, 1 < / RTI > MTT assays. The control group was not treated with anything and the negative control group was treated with 0.2 mM corticosterone for 48 hours.

2 shows the protective activity of nerve cells from corticosterone of water and ethanol extract of ginseng seeds (Control: untreated group, con-1: corticosterone treatment (0.2 mM, 48 hr) 50; 50% EtOH ginseng seed extract; 80; 80% EtOH ginseng seed extract). As shown in FIG. 2, the cell activity was lowered to about 60% due to the oxidative stress induced by corticosterone and showed a high protective activity in the ginseng seed extract.

Experimental Example  3. Corticosterone  Effect of Neural Cell Growth Factors on Neurotoxicity Induced by Neuronal Cells

Experiments were carried out in order to confirm whether the ginseng seed extract obtained in the above Examples promotes the expression of nerve cell growth factor from corticosterone-induced neuronal toxicity.

PC12 cells were cultured in 6-well plate in RPMI-1640 medium containing antimicotics / antibiotics. When the number of cells was 2x10 ^{5 per} well, the ginseng seed extract obtained in the above Example was dissolved in 5% DMSO After dissolving, 15 μl of each solution was pre-incubated at 37 ° C for 2-4 hours, and 0.2 mM corticosterone was treated for 48 hours to induce neuronal cytotoxicity. Growth nutrients and related factors were measured. Controls did not process any samples, and negative control (control-1) was measured by treatment with corticosterone alone.

The brain-derived neurotrophic factor (BDNF) used as a neurotrophic factor and related factor in this experiment is a major factor affecting the survival and expression of neuronal cells as a major neurotrophic factor. CRB (cyclic adenosine monophosphate response element nindint protein) is known to be a factor that closely affects cell signaling pathway and BDNF expression. MAP-4 (microtubule-associated protein 4) is known to be a non-neuronal micortubule-associated protein closely related to the expression of CREB and is known to be associated with the cell cycle.

FIG. 3 shows that water and ethanol extracts of ginseng seed improve the expression of nerve cell nutrients inhibited by corticosterone (Control: untreated control, control-1: corticosterone treatment (0.2 mM, 48 hr) Ginseng seed extract, 50% EtOH ginseng seed extract, 80; 80% EtOH ginseng seed extract). As shown in FIG. 3, the neuronal cell toxicity induced by corticosterone lowered the expression level of neuronal cell growth factor and related factor to about 40-60%. The inhibitory effect of the ginseng seed extract on the expression of related factors Was improved. .

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  One. Sanje  Produce

Ginseng seed hydrothermal or ethanol extract 20 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation example  2. Preparation of tablets

Ginseng seed hot water or ethanol extract 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

Ginseng seed hot water or ethanol extract 10 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed in accordance with a conventional method for producing a capsule, and filled in a gelatin capsule to prepare a capsule.

Formulation example  4. Preparation of injections

Ginseng seed hot water or ethanol extract 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

(2 ml) per ampoule in accordance with the usual injection method.

Formulation example  5. Liquid  Produce

Ginseng seed hydrothermal or ethanol extract 20 mg

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation example  6. Manufacture of health food

Ginseng seeds Hot water or ethanol extract 1000 mg

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

24.8 mg of magnesium chloride

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation example  7. Manufacture of health drinks

Ginseng seeds Hot water or ethanol extract 1000 mg

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (12)

A pharmaceutical composition for the prevention and treatment of neuronal cell protection and depression comprising ginseng seed extract as an active ingredient.
The method according to claim 1,
Wherein the ginseng seed extract is an extract of ginseng seeds, an organic solvent or a mixture thereof.
3. The method of claim 2,
Wherein the organic solvent is at least one solvent selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether.
The method according to claim 1,
Wherein the ginseng seed extract is a lower alcohol extract of ginseng seed.
The method according to claim 1,
Wherein the ginseng seed extract is an ethanol extract of ginseng seed.
The method according to claim 1,
Wherein the ginseng seed extract is a hexane extract of ginseng seed.
The present invention relates to a food composition for preventing and ameliorating neuronal cell protection and depression comprising ginseng seed extract as an active ingredient.
8. The method of claim 7,
Wherein the ginseng seed extract is an extract of ginseng seeds, an organic solvent or a mixture thereof.
9. The method of claim 8,
Wherein the organic solvent is at least one solvent selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether.
8. The method of claim 7,
Wherein the ginseng seed extract is a lower alcohol extract of ginseng seed.
8. The method of claim 7,
Wherein the ginseng seed extract is an ethanol extract of ginseng seed.
8. The method of claim 7,
Wherein the ginseng seed extract is a hexane extract of ginseng seed.

Images