KR102275824B1 - The anti-diabetes composition containing extract mixture and manufaturing method thereof - Google Patents

Abstract

The present invention relates to a complex natural product composition exhibiting an effect of preventing and alleviating diabetes and a method for manufacturing the same, and more specifically, to a food composition for preventing and alleviating diabetes, including a mulberry extract, a Schisandra chinensis extract and a Calamagrostis epigejos seed extract, and a method for manufacturing the same. A complex extract composition manufactured by the manufacturing method of the present invention is excellent in preventing and alleviating diabetes, and can be stored stably for a long time. In addition, the composition is highly palatable and can be enjoyed by people of all ages and genders.

Description

Complex natural product composition for preventing and improving diabetes and manufacturing method thereof {The anti-diabetes composition containing extract mixture and manufaturing method thereof}

The present invention relates to a complex natural product composition exhibiting an effect of preventing and improving diabetes, and a method for manufacturing the same, and more particularly, to a food composition for preventing and improving diabetes comprising a heartburn extract, a Schisandra extract and a sanjo seed extract, and a method for preparing the same it's about

Diabetes mellitus is a chronic metabolic disease, and over a long period of time, it causes vascular disorders and dysfunction of nerves, kidneys, and retina, and this is a disease that leads to loss of life. Diabetes is largely divided into insulin-dependent diabetes mellitus (type 1 diabetes) and non-dependent diabetes mellitus (type 2 diabetes) according to the mechanism of occurrence.

Insulin-dependent diabetes mellitus is known to occur due to inability to consume blood glucose because pancreatic beta cells are selectively damaged or destroyed and the body cannot produce insulin. indicates It occurs frequently in children, and its treatment method is the administration of insulin.

Non-insulin-dependent diabetes mellitus accounts for more than 90% of diabetic patients and mainly occurs in adults. However, the exact cause has not been identified, but it is estimated that it is caused by a combination of genetic factors and environmental factors such as abnormal diet, stress, exercise, obesity, and aging. The non-insulin-dependent diabetes mellitus generally exhibits resistance to insulin, and a hyperglycemic state usually persists due to insulin insufficiency. If it progresses chronically, complications such as retinopathy, nephropathy, neuropathy, arteriosclerosis, infection and osteopenia appear.

Currently, diet therapy, exercise therapy, sulfonylurea drugs, biguanide-based drugs, α-glucosidase inhibitors, and insulin are used as treatment methods for non-insulin-dependent diabetes mellitus. Litinide-based, thiazolidinidion-based drugs, and insulin with various action times have been developed and applied to clinical practice. However, these therapeutic agents have a problem in that they have low efficacy or cause many side effects, such as liver dysfunction, hypoglycemia, and lactic acidemia. Therefore, there is an urgent need to develop a diabetes treatment agent that reduces these side effects, improves metabolic abnormalities, and has excellent safety even when taken for a long time.

On the other hand, heartburn is a mature fruit of Morus alba L belonging to the family Moraceae and a closely related plant.

Currently, studies on the physiological activity of mulberry-derived products have mainly isolated the glycoprotein, moran A, extracted from the epidermis and reported it as a hypoglycemic agent (Hikino et al.; Planta Medica, 51, pp159-256). Although there are reports of the blood sugar lowering effect of the broken heart as a result of modern medical research, there is still insufficient research on the extract combination that works together with the heartburn extract to enhance the blood sugar lowering action.

Korean Patent No. 1908772

Although there have been reports on the blood sugar lowering effect of the heartbroken, studies on extract combinations that work together with the heartburn extract to enhance the blood sugar lowering action are still insufficient.

Accordingly, the present invention was completed by confirming that, in addition to the heartburn extract, Cuji mulberry fruit extract, Schisandra fruit extract and Sanjo seed extract were additionally included, the blood sugar lowering effect after a meal was improved and palatability was also increased.

It is an object of the present invention to provide a food composition for preventing and improving diabetes, comprising a heartburn extract, a omija extract and a sanjo seed extract.

Another object of the present invention is to provide a method for preparing a food composition comprising the extract.

The present invention for achieving the object as described above provides a food composition for preventing and improving diabetes, comprising a heartburn extract, a omija extract and a sanjo seed extract.

The extract may be a hot water extract.

The extract may be an extract extracted with hot water at 90 - 95 ° C. for 6 - 24 hours.

The extract may further include white ginseng, mulberry fruit, ginseng root, namgasae fruit, and radish seeds or both.

The mixing ratio of each of the above natural products may be heartburn: omija: sanjo seed = 1.3 - 1.7: 1:1.

In order to achieve another object of the invention, (a) heartworm 1.716%, mulberry fruit 1.225%, omija fruit 1.225%, sanjo seed 1.225%, white ginseng 0.735%, ginseng root 0.735%, namgasae fruit 0.735% and radish seed compounding at a weight compounding ratio (w/w) of 0.735%; (b) extracting an extract by hot water extraction at 90 - 95 °C for 6 - 24 hours; and (c) filtering and drying; provides a method for preparing a food composition for preventing and improving diabetes, including.

After the filtration in step (c), a microfluidizer treatment may be additionally performed.

The present invention relates to a food composition for preventing and improving diabetes, comprising a heartburn extract, a Schisandra extract and a sanjo seed extract, and a method for preparing the same. The complex extract composition prepared by the manufacturing method of the present invention is excellent in preventing and improving diabetes, and can be stored stably for a long time. It is also highly palatable and can be enjoyed by people of all ages and genders.

In order to fully understand the present invention, it will be described with reference to preferred embodiments of the present invention. Embodiments of the present invention may be modified in various forms, and the scope of the present invention should not be construed as being limited to the embodiments described in detail below. This example is provided to more completely explain the present invention to those of ordinary skill in the art.

Throughout this specification, when a part "includes" a certain component, it does not exclude other components unless otherwise stated, and may further include other components.

The present invention provides a food composition for preventing and improving diabetes, comprising the heartburn extract, Schisandra extract and sanjo seed extract.

Heartburn (Audrey) is the fruit of Morus alba L., a deciduous tree belonging to the family Moraceae, and contains sugars, fatty acids such as tannic acid, vitamins, and linolic acid. known to contain. These heartbroken ones are collected when they turn from blue to purple and black, and are known to be effective for dizziness, tinnitus, dry mouth, sogal, tonic, pain reliever, insomnia, back pain, and constipation, and can be used for food and medicine.

Mija is a fruit that grows on a deciduous tree of the Omija family. It is called Omija because it has five flavors: sweet, bitter, sour, spicy, and salty. Omija is generally known to have the effects of improving blood flow, preventing high blood pressure, stroke, cardiovascular disease, improving immunity, preventing diabetes, improving liver function, restoring energy, and improving respiratory diseases. On the other hand, omija according to an embodiment of the present invention may be a by-product of omija fruit, skin or omijacheong.

Sanjo seeds are also called sanjoin. Sanjo seeds are the husks of wild jujube seeds, which are commercialized as a treatment for depression in oriental medicine, and are generally used after roasting.

In addition, the extract as defined herein includes a solvent selected from water including purified water, lower alcohols having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably water or a mixed solvent of water and ethanol, more preferably water. The extract of the present invention may be an extract extracted with hot water.

It may be extracted for 6 to 24 hours, preferably for 12 hours by adding water or a mixed solvent of 50 - 99% water and ethanol. If the extraction is less than 6 hours, the active ingredient may not be sufficiently extracted, and if it is extracted for more than 24 hours, the active ingredient may be denatured or unnecessary ingredients may be extracted excessively.

The extract of the present invention is extracted by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. at 10°C to 150°C, preferably at 20°C to 100°C, more preferably at 90°C to 95°C, preferably , a first step of obtaining an extract by performing a cold extraction method or a hot water extraction method; Individual extracts of the present invention can be obtained through the second-step manufacturing process of filtering the extract obtained above and drying the filtrate. Most preferably, the extract may be an extract extracted with hot water at 90 - 95 °C for 6 - 24 hours.

The extract may further include or all of Cuji mulberry fruit, ginseng root, namgasae fruit and radish seeds.

Cudrania is a deciduous small arboreous tree belonging to the genus Cudraniaceae, and it is commonly distributed in southern regions of Korea such as Gyeongnam, Jeollanam-do, and Jeju. In addition, edible leaves and fruits contain anticancer components, and are used as folk remedies for gastric cancer, esophageal cancer, and liver cancer.

Salviae Miltiorrhizae Radix is a perennial plant of the Lamiaceae family. It utilizes dried roots and is mainly used for cardiovascular system, sepsis, and sleep disorders.

Namgasae ( Tribulus terrestris ) is also called by other names such as tiredness. Stems branch from the bottom, split and grow sideways, reaching about 1m in length. The leaves are 1-6 cm long, have short stalks, and have small lanceolate triangular stipules. The small leaves are long oval and both sides are not the same, and there are white eye hairs behind the leaves. The place of origin is known as Korea, India, and Taiwan. It is known to be effective for high blood pressure, ophthalmic diseases, and liver protection.

Radish seeds are also called lepers. Leprosy is a plant belonging to the cruciferous family and is the ripening seed of radish (Raphanus sativus L.). Dried seeds are oval or round ovate, slightly flattened, about 3mm long and 2.5mm wide. The surface is reddish-brown, with several longitudinal grooves on one side and an ovule at one end. The ovule is brown and has a round dot shape. When observed with a magnifying glass, the entire seed has fine net patterns. The quality is hard and when it is cracked, yellowish-white or yellowish grains are visible and the grains contain essential oil. In summer and autumn, when the seeds are ripe, cut the vines, dry them in the sun, rub them with your hands to remove the seeds, remove foreign substances, and dry them in the sun to use as herbal medicine.

The extract may additionally include any one or more selected from the group consisting of gold, ginseng, geunyul, jainja, chrysanthemum, white ginseng, and cheongho.

According to Examples 5 to 7, when gold, ginseng, dryness, traveler, chrysanthemum, white ginseng and cheongho were additionally included, better blood sugar lowering effect (Tables 4 and 5), storage capacity (Table 6) and sensuality (Table 7) ) was confirmed to be visible.

The mixing ratio of each of the above natural products may be heartburn: omija: sanjo seed = 1.3 - 1.7: 1: 1: 1: 1.

The present inventors have confirmed that when prepared in the blending ratio of Example 1, it has the highest preservability and optimal blood sugar lowering effect and sensory properties.

The present inventors conducted experiments on various formulations and mixing ratios of each extract (see Examples 1 to 10). It was confirmed that a better blood sugar lowering effect was obtained when namgasae fruit, radish seeds, cucurbita fruit and dandelion root were added. In addition, it was confirmed that the preservation and sensuality were improved when gold, sasan, geonyul, jainja, chrysanthemum, white ginseng and cheongho were added.

Diabetes as defined herein also includes type 1 diabetes and type 2 diabetes, preferably type 2 diabetes.

As a result of performing a blood glucose measurement experiment using the db/db mouse model of the extract of the present invention obtained by the above method, an excellent blood glucose lowering effect was confirmed.

In addition, the complex natural product extract of the present invention is a drug that has been edible or used as a herbal medicine for a long time, and the extracts of the present invention extracted therefrom also do not have problems such as toxicity and side effects.

The present invention provides a health functional food for preventing and improving diabetes containing the complex natural product extract of the present invention having a hypoglycemic effect as an active ingredient.

"Health functional food" as defined herein means a food manufactured and processed using raw materials or ingredients useful for the human body according to Act No. 6727 of the Health Functional Food Act. It refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients with respect to structure and function or physiological effects.

The health functional food for preventing diabetes and diabetic complications of the present invention contains the extract in an amount of 0.01 to 95%, preferably 1 to 80% by weight based on the total weight of the composition.

In addition, it is possible to manufacture and process health functional foods in the form of tablets, capsules, powders, granules, liquids, pills, etc. for the purpose of preventing diabetes and diabetic complications.

The present invention provides a dietary supplement comprising the extract showing the effect of improving blood sugar, improving lipid metabolism, and preventing and treating diabetes and diabetic complications. Foods to which the extract can be added include, for example, various foods, beverages, gum, tea, vitamin complexes, health functional foods, and the like.

In addition, it may be added to food or beverage for the purpose of improving blood sugar, improving lipid metabolism, and preventing and treating complications of diabetes and diabetic complications. At this time, the amount of the extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, and the health beverage composition may be added in a ratio of 0.02 to 5g, preferably 0.3 to 1g based on 100 ml. .

The health functional beverage composition of the present invention is not particularly limited in other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like. In addition, the sample of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the sample of the present invention.

The complex natural extract of the present invention can also be used as a pharmaceutical composition for preventing and treating diabetes and diabetic complications.

The pharmaceutical composition for preventing and treating diabetes and diabetic complications containing the complex natural extract of the present invention contains the extract in an amount of 0.1 to 50% by weight based on the total weight of the composition.

The pharmaceutical composition comprising the extract of the present invention may further include suitable carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical dosage form of the extract of the present invention may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds as well as in an appropriate group.

The pharmaceutical composition comprising the extract according to the present invention is in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injection solutions, respectively, according to conventional methods. It can be formulated and used. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate , cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the extract, for example, starch, calcium carbonate, sucrose ) or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. As the base of the suppository, Witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, etc. may be used.

The preferred dosage of the extract of the present invention varies depending on the condition and weight of the patient, the degree of disease, the drug form, the route and duration of administration, but may be appropriately selected by those skilled in the art. However, for a desirable effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg/kg per day, preferably at 0.001 to 100 mg/kg. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

The extract of the present invention may be administered to mammals such as rats, mice, livestock, and humans by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection. Most preferably, it may be an oral administration method.

The term definitions for the excipients, binders, disintegrants, lubricants, flavoring agents, and the like of the present invention are those described in documents known in the art and include those having the same or similar functions (Explanation of the Korean Pharmacopoeia, Moonseongsa) , Korea Association of Pharmacy Colleges, 5th edition, p33-48, 1989).

In order to achieve another object of the present invention, (a) 1.716 chestnut berry, 1.225 mulberry fruit, 1.225 omija fruit, 1.225 sanjo seed 1.225, white ginseng 0.735, ginseng root 0.735, namgasae fruit 0.735 and radish seed 0.735 in a weight ratio step; (b) extracting an extract by hot water extraction at 90 - 95 °C for 6 - 24 hours; and (c) filtering and drying; provides a method for preparing a food composition for preventing and improving diabetes, including.

After the filtration in step (c), a microfluidizer treatment may be additionally performed. Example 11 is an extract treated with a microfluidizer, and Example 11 exhibited a better blood sugar lowering effect than that of Example 1. Since the aggregated particles between the active ingredients in the extract are pulverized to prepare a homogeneous dispersion, it is understood that a better effect is exhibited.

Hereinafter, a manufacturing method according to an embodiment of the present invention will be described in detail.

<Example 1>

The extract was obtained by mixing 1.716% chestnuts purchased at Gyeongdong Market (Seoul), 1.225% omija fruit, and 1.225% sanjo seeds in a weight mixing ratio (w/w) and extracting it with boiling water at 95°C for 12 hours to obtain an extract, which was filtered and dried. to obtain a natural product complex extract and used in the following experiment.

<Examples 2 - 4>

division heartbroken Schisandra sanjo seeds white man namgasae fruit radish seeds goujippong fruit ginseng root Example 1 1.7 1.2 1.2 N/A N/A N/A N/A N/A Example 2 1.7 1.2 1.2 0.7 0.7 N/A N/A N/A Example 3 1.7 1.2 1.2 0.7 0.7 0.7 N/A N/A Example 4 1.7 1.2 1.2 0.7 0.7 0.7 1.2 0.7

* Numbers in the table indicate the weight mixing ratio (w/w)

A natural product complex extract was obtained in the same manner as in Example 1, except that the natural products described in Table 1 were additionally used.

<Examples 5 - 7>

division Example 4 Configuration Gold four or three dry rate traveler Chrysanthemum Cheongho Example 5 include 0.7 0.5 0.5 N/A N/A N/A Example 6 include N/A N/A N/A 0.7 0.5 0.5 Example 7 include 0.5 0.5 0.5 0.5 0.5 0.5

* Numbers in the table indicate the weight mixing ratio (w/w)

A natural product complex extract was obtained in the same manner as in Example 1, except that the natural products described in Table 2 were additionally used.

<Examples 8 - 10>

division heartbroken Schisandra sanjo seeds Example 8 1.7 1.7 1.7 Example 9 0.5 0.5 1.7 Example 10 1.7 1.2 0.5

* Numbers in the table indicate the weight mixing ratio (w/w)

A natural product complex extract was obtained in the same manner as in Example 1, except that natural products were mixed and used in the weight mixing ratio of chestnut seed, omija and sanjo seeds described in Table 3 above.

<Example 11>

The extract was obtained by mixing 1.716% chestnuts purchased at Gyeongdong Market (Seoul), 1.225% omija fruit, and 1.225% sanjo seeds in a weight mixing ratio (w/w), followed by hot water extraction at 95°C for 12 hours to obtain an extract, which was filtered. . After filtration, a step of preparing a homogeneous dispersion by pulverizing the agglomerated particles using a microfluidizer (ZEUS, M-110P) was additionally performed. Thereafter, it was dried to obtain a natural product complex extract and used in the following experiment.

<Comparative Example 1>

Comparative Example 1 was prepared without adding a separate natural extract in the preparation method of <Example 1>.

<Experimental Example 1>

Confirmation of hypoglycemic effect on normal rats

Normal mice 3 weeks old (weight 25 ± 2 g) were received and acclimatized in an animal breeding room for 1 week, and then 2 mice were assigned to each experimental group. Two animals per group were divided into 12 groups (Example 1-11 and Comparative Example 1). At 4 weeks of age, blood glucose measurements were performed at 1 day intervals for a total of 3 days.

And the natural product complex extract was orally administered at 10 am and 16 pm every day by using a mouse sonde. Blood glucose was measured twice a day, 10 minutes after breakfast and 10 minutes after taking the extract. Blood glucose was measured in the tail vein of the mouse with a portable glucometer (OneTOUCH@Ultra, Johnson & Johnson, USA).

The measured results are shown in Table 4.

division Blood sugar level 10 minutes after eating
(mg/dL) Blood sugar level after taking the extract
(mg/dL) decline rate
(%) division Blood sugar level 10 minutes after eating
(mg/dL) Blood sugar level after taking the extract
(mg/dL) decline rate
(%) Example 1 250 211 16 Example 2 269 222 17 Example 3 264 215 19 Example 4 240 195 19 Example 5 241 195 19 Example 6 256 206 20 Example 7 230 175 24 Example 8 244 215 12 Example 9 267 235 12 Example 10 301 256 15 Example 11 288 201 30 Comparative Example 1 294 281 4

* Describe the average value of two mice As can be seen from the results shown in Table 4, the dietary supplement of the present invention exhibits a hypoglycemic effect in normal mice.

<Experimental Example 2>

Confirmation of anti-diabetic effect using diabetic animal model

It was performed to verify the preventive or therapeutic effect of antidiabetic and antihyperlipidemia in a representative animal model of type 2 diabetes for comparative verification of the complex natural extract of the present invention with the existing drug metformin.

BKS.Cg-m ^+/+ Lepr ^db/J homozygousdiabetic ( db/db) mice (20g, central experimental animal) 3 weeks old (weight 20 ± 2g) were received and acclimatized in the animal breeding room for 1 week. were assigned to each. Two animals per group were divided into 13 groups (Example 1-11 and Comparative Example 1-2). In Comparative Example 2, metformin (150 mg/kg/day) was administered as a positive control.

At 4 weeks of age, blood glucose measurements were performed at 1 day intervals for a total of 3 days.

And the natural product complex extract was orally administered at 10 am and 16 pm every day by using a mouse sonde. Blood glucose was measured twice a day, 10 minutes after breakfast and 10 minutes after taking the extract. Blood glucose was measured in the tail vein of the mouse with a portable glucometer (OneTOUCH@Ultra, Johnson & Johnson, USA).

The measured results are shown in Table 5.

division Blood sugar level 10 minutes after eating
(mg/dL) Blood sugar level after taking the extract
(mg/dL) decline rate
(%) division Blood sugar level 10 minutes after eating
(mg/dL) Blood sugar level after taking the extract
(mg/dL) decline rate
(%) Example 1 321 263 18 Example 2 365 296 19 Example 3 311 249 20 Example 4 420 332 21 Example 5 301 238 21 Example 6 341 266 22 Example 7 230 170 26 Example 8 356 303 15 Example 9 267 230 14 Example 10 296 252 15 Example 11 306 211 31 Comparative Example 1 305 290 5 Comparative Example 2 342 263 23

* Write the average value of two mice

<Experimental Example 3>

storage capacity evaluation

After storing the complex natural product extracts prepared in the above Examples and Comparative Examples at a storage temperature of 30° C. for 10 days, the degree of occurrence of floating matter and the odor of decay were confirmed.

The degree of occurrence of suspended solids was measured with an absorptiometer, and it was confirmed whether insoluble suspended solids were generated compared to the existing ones. A significant increase in the absorbance value compared to the previous value is indicated by +++, and a case with little change from the initial value is indicated by -.

The odor of putrefaction was measured using an odor meter (OMX-SRM). It can be judged that the higher the number, the more severe the smell of decay.

The storage stability evaluation results are shown in Table 6 below.

division Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 floatation + + + - - - rot 17 15 15 14 19 16 division Example 7 Example 8 Example 9 Example 10 Example 11 floatation - + + + + rot 17 16 15 16 17

Looking at Table 6, the generation of suspended matter was made less or hardly occurred in the complex natural product extract as an example. In addition, the odor of decay was also confirmed to be relatively low. It is judged that the addition of a natural extract, a natural microorganism inhibitor, suppresses the generation of floating matter and prevents the rotting odor.

<Experimental Example 4>

sensory test

A sensory test was performed on the complex natural product extract prepared in Examples 1-11. The extracts of Examples 1-11 were processed into beverages, and the taste, smell, and overall preference of the product (5 = very good, 3 = neither good nor bad, 1 = very bad) were selected by 5 panel personnel, It was tested on a 5-point scale.

Each numerical value for the following sensory test items is a value rounded to the first decimal place after dividing the sum of the scores of sensory test agents by the number of sensory test agents.

division Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 sensuality 10 10 11 12 12 11 division Example 7 Example 8 Example 9 Example 10 Example 11 sensuality 14 9 8 10 15

Looking at Table 7, it was found that the complex natural product extract prepared in Example 11 had the highest functionality.

<Formulation Example 1>

Preparation of powders

20 mg of the natural product complex extract of the present invention

Lactose 100 mg

talc 10 mg

A powder is prepared by mixing the above ingredients and filling an airtight bag.

<Formulation Example 2>

manufacture of tablets

10 mg of natural product complex extract of the present invention

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

<Formulation Example 3>

Preparation of capsules

10 mg of natural product complex extract of the present invention

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

<Formulation Example 4>

manufacture of injections

10 mg of natural product complex extract of the present invention

mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO ₄ 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule (2 ml) is prepared.

<Formulation Example 5>

Preparation of liquids

20 mg of the natural product complex extract of the present invention

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 ml by adding purified water, and then filled in a brown bottle. Sterilize to prepare a solution.

<Formulation Example 6>

manufacturing of health food

1000 mg of the natural product complex extract of the present invention

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is a composition that is relatively suitable for health food in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the ingredients are mixed according to a conventional health food manufacturing method. , to prepare granules, and can be used for preparing health food compositions according to a conventional method.

<Formulation Example 7>

manufacture of health drinks

1000 mg of the natural product complex extract of the present invention

citric acid 1000 mg

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 ml by adding purified water

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L container, sealed and sterilized, and then refrigerated. used in the manufacture of health beverage compositions.

<Formulation Example 8>

Mixed extract powder health food

Prepare mixed extract powder health food mixed in the following mixing ratio.

Dextrin 91.179%

Natural product complex extract of the present invention 8.821% (1.716% of chestnut extract powder, 1.225% of mulberry extract powder, 1.225% of Schisandra fruit extract powder, 1.225% of sanjo seed extract powder, 0.735% of ginseng root extract powder, 0.735% of white ginseng, Fruit Extract Powder 0.735%, Radish Seed Extract Powder 0.735%)

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

A food composition for preventing and improving diabetes, characterized in that it contains a heartburn extract, a Schisandra extract and a sanjo seed extract, and the weight mixing ratio of each natural product is heartburn: omija: sanjo seed = 1.3 - 1.7: 1:1.
delete [Claim 2] The food composition for preventing and improving diabetes according to claim 1, wherein the food composition further comprises a ginseng root extract, namgasae fruit extract, and radish seed extract.
(a) step of mixing in a weight mixing ratio of 1.716 heartburn, 1.225 mulberry fruit 1.225, omija fruit 1.225, sanjo seed 1.225, white ginseng 0.735, dansam root 0.735, namgasae fruit 0.735 and radish seed 0.735;
(b) obtaining an extract by hot water extraction with water at 90 - 95 °C for 6 - 24 hours; and
(C) filtration and drying step; diabetes prevention and method for producing a food composition for improvement comprising a.