KR102366919B1 - Functional health food composition for inhibiting muscle reduction comprising a mixed extract of mulberry twig, Eucommia bark, Acanthopanax, and black bean - Google Patents

Abstract

The present invention relates to a functional health food composition for inhibiting muscle reduction comprising a mixed extract of mulberry twig, eucommia bark, acanthopanax, and black bean. A health food composition according to the present invention has no cytotoxicity and exhibits an effect of increasing the survival rate of muscle cells due to oxidative stress, and thus can be usefully used to prevent or improve muscle reduction related diseases such as sarcopenia.

Description

Functional health food composition for inhibiting muscle reduction comprising a mixed extract of mulberry twig, Eucommia bark, Acanthopanax, and black bean

The present invention relates to a functional health food composition for inhibiting muscle loss comprising a mixed extract of upper extremity, cephalothorax, oleracea and black bean.

Recently, Korea has already entered an aged society due to a decrease in the fertility rate and an extension of life expectancy, and a super-aged society is approaching. According to data from the National Statistical Office, the elderly population aged 65 and over will account for 16.5% of the population in 2021 and is expected to exceed 25% within 10 years.

There are various physiological changes with aging, and one of the most common changes is a decrease in muscle mass.

Skeletal muscle is the largest organ constituting the human body and occupies 40% to 50% of the human body. It is known that the skeletal muscle tends to decrease by about 1% every year in our body after the age of 30, but decreases sharply after the age of 65, which is about half that of those in their 30s. As aging progresses, the functional ability of muscles (muscle strength, power) also decreases gradually. This decrease in muscle causes daily inconveniences such as changes in body shape and difficulty in maintaining posture, as well as lowering basal metabolic rate and thus Ultimately, it is attracting attention as a cause of adult diseases including obesity, diabetes, and hyperlipidemia by overcoming the imbalance between energy supply/consumption.

Sarcopenia is a compound word of 'Sarco', which means muscle, and 'penia', which means reduced muscle, which is derived from the Greek word, and refers to a decrease in muscle mass associated with aging. This aging process was considered only a natural phenomenon in the past, but since the World Health Organization (WHO) assigned a disease code (ICD-10-CM M62.84) to senile sarcopenia in 2016, senile sarcopenia has been regarded as a disease.

Recently, it is recognized that the sarcopenia has a serious impact on the occurrence of metabolic diseases, functional disorders, quality of life, medical costs, etc., and various efforts are being made to prevent and improve the sarcopenia. Research on the cause of sarcopenia and its correlation with the occurrence of adult diseases is being actively conducted.

Therefore, as the importance of improving muscle health for the prevention of metabolic diseases, aging prevention, and improvement of quality of life has emerged, the search for functional materials is being actively conducted.

Due to such social interest, many studies are being carried out in anticipation to develop natural functional materials of plant origin that are effective in preventing sarcopenia and are safe and economical. am.

Accordingly, the present inventors conducted a study to develop an effective naturally-derived ingredient for the prevention, treatment or improvement of sarcopenia. It was found that there is a synergistic effect on the inhibition of muscle loss and completed the present invention.

Korean Patent Publication No. 10-2110040

It is an object of the present invention to provide a health food composition for preventing or improving muscle loss-related diseases, comprising a mixed extract of upper extremity, cephalosporidium, oleracea and black bean.

Another object of the present invention is to provide a health functional food composition for the prevention or improvement of muscle loss-related diseases comprising a mixed extract of upper extremity, cephalosporidium, oleracea and black bean.

Another object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of muscle loss-related diseases, comprising a mixed extract of upper extremity, cephalothorax, oleander and black bean.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. There will be.

In order to achieve the above object,

An object of the present invention is to provide a health food composition for preventing or improving muscle loss-related diseases, comprising a mixed extract of upper extremity, cephalothorax, oleander and black bean.

In addition, the present invention provides a health functional food composition for the prevention or improvement of muscle loss-related diseases comprising a mixed extract of upper extremities, cephalothorax, oleracea and black bean.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of muscle loss-related diseases, comprising a mixed extract of upper extremity, cephalothorax, oleracea and black bean.

The health food composition for the prevention or improvement of muscle reduction-related diseases, comprising a mixed extract of upper extremity, cephalothorax, oleander and black bean according to the present invention, has no cytotoxicity and has an effect of increasing the survival rate of muscle cells due to oxidative stress. As such, it can be usefully used to prevent or improve muscle loss-related diseases such as sarcopenia.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a graph showing the cell viability evaluation result of a mixed extract of upper extremity, cephalosporidium, oleracea and black bean of the present invention and a comparative example.
2 a) is a graph showing the measurement results of cell viability by concentration in myoblasts due to the treatment of the mixed extract of upper extremity, cephalosporidium, oleracea and black bean of the present invention, b) is the upper extremity, cephalothorax of the present invention , It is a graph showing the measurement results of cell viability of myoblasts when the mixed extracts of oleracea and black bean and Comparative Examples were treated at the same concentration.
Figure 3a) is a graph showing the measurement results of cell viability by concentration in the myotube caused by the treatment of the mixed extract of upper extremity, cephalosporidium, oleracea and black bean of the present invention, b) is the upper extremity of the present invention, It is a graph showing the measurement results of the cell viability of myotubes when the mixed extracts of oleander and black bean and the comparative example were treated at the same concentration.

Hereinafter, the present invention will be described in more detail. However, the present invention may be embodied in various different forms, and the present invention is not limited by the embodiments described herein, and the present invention is only defined by the claims to be described later.

In addition, the terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. In the entire specification of the present invention, 'including' any component means that other components may be further included, rather than excluding other components, unless otherwise stated. In addition, the 'comprising' also means using the component alone.

Food and health functional food composition

One aspect of the present invention provides a health food composition for preventing or improving muscle loss-related diseases, comprising a mixed extract of upper extremities, cephalothorax, oleander and black bean.

In the present specification, the upper limb (mulberry twig) means a dried tender branch of a plant of the genus Morus, has a slightly bitter taste, mainly collected between late spring and early summer, and has anti-inflammatory, diuretic, tonic, sweating and wind-removing efficacy It has been used as a herbal medicine for a long time.

In addition, Eucommia bark means a deciduous tree belonging to Eucommiaceae or a crude drug obtained by drying the bark thereof. The stems and leaves have been consumed as tea since ancient times, and it contains a lot of vitamin C and is effective in preventing heart disease, diabetes, and arteriosclerosis as well as enhancing the function of the adrenal glands. The stems and leaves have been consumed as herbal medicines useful for various diseases such as high blood pressure, stress, arthritis, and hangover. Its value is being recognized again.

Also, Acanthopanax refers to the bark of Acanthopanax sessiliflorum Seeman or its roots, stems and branches belonging to the Araliacease family. According to Donguibogam, Ogapi is the dried bark of Acanthopanax Sessilifloum Seem, and it grows in the foothills and valleys of the mountain. It is said that the fruit of ogapi cures male genital warts and genital pruritus, and eliminates paralysis and burns. It has been recorded that it has many effects such as protecting the stomach, improving energy, and purifying the blood, and has been used as a tonic in oriental medicine for a long time. It is known as a herbal medicine that has been used to treat diseases such as beriberi, beriberi and musculoskeletal weakness.

Also, black bean means black bean. Soybeans are edible crops of the Rosaceae legume family, dicotyledonous plants, and are complete protein foods containing 35% to 40% protein. Soy protein has the advantage of being absorbed faster than general proteins and amino acids and providing protein nutrition without burdening the stomach. is being reported In particular, black beans have a similar nutrient content compared to regular beans, but contain four times as many anti-aging ingredients, and are known to be more effective in preventing adult diseases and losing weight.

The present inventors have completed the present invention by finding out that the mixed extracts of upper extremity, cephalosporidium, oleracea, and black bean, each having different efficacy, are effective in protecting muscle cells.

In one embodiment of the present invention, the mixture may contain 10 to 30 parts by weight of the cephalosporus, 10 to 30 parts by weight of the scallops, and 10 to 30 parts by weight of the black bean, based on 100 parts by weight of the upper extremity.

In an embodiment of the present invention, the mixture may contain 17 to 23 parts by weight of the cephalosporus, 17 to 23 parts by weight of the scallops, and 17 to 23 parts by weight of the black bean, based on 100 parts by weight of the upper extremity.

In an embodiment of the present invention, the mixture may contain 19 to 21 parts by weight of the cephalothorax, 19 to 21 parts by weight of the scallops, and 19 to 21 parts by weight of the black bean, for example, based on 100 parts by weight of the upper extremity. For example, based on 100 parts by weight of the upper extremity, 20 parts by weight of the cephalothorax, 20 parts by weight of the ogapi, and 20 parts by weight of the black bean may be included.

In one embodiment of the present invention, the mixed extract may be obtained by extracting the mixture with water, a linear or branched chain alcohol having 2 to 4 carbon atoms, or a mixture thereof, for example, water, A solvent capable of extracting the mixture is not limited as long as it is obvious in the art.

In one embodiment of the present invention, the muscle loss-related disease occurs due to muscle loss induced by oxidative stress, for example, sarcopenia, muscular atrophy, muscular dystrophy, atonia and myasthenia gravis selected from the group consisting of It may be one or more, for example, but not limited to, sarcopenia.

The health food composition of the present invention is safe without cytotoxicity, and has excellent protective effect against oxidative stress, and has excellent cytoprotective effect from oxidative stress, so it can be used for preventing or treating muscle loss, furthermore, It can be used as a functional composition for strengthening and repairing oxidative muscle damage.

One aspect of the present invention also provides a health functional food composition for the prevention or improvement of muscle loss-related diseases, comprising a mixed extract of upper extremities, head, head, and black bean.

In one embodiment of the present invention, the mixed extract of the upper extremity, cephalothorax, serrata and black bean may be the same as the mixed extract of the above aspect, and the description of the mixed extract is replaced by the description of the above aspect.

There are no special restrictions on the type of food. Examples of foods to which the active substance of the present invention can be added include drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, There are various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and processed dairy products, and includes both health food and health functional food in the ordinary sense.

The health food and health functional food composition containing the active substance according to the present invention may be added to food as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active substance may be suitably determined according to the purpose of its use (for prevention or improvement). In general, the amount of the composition in health food and health functional food may be added in an amount of 0.1 to 90 parts by weight based on the total weight of the food. However, in the case of long-term intake for health maintenance or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

The health food and health functional food composition of the present invention is not particularly limited in other ingredients except that it contains the active substance of the present invention as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates are added as additional ingredients like conventional beverages. may contain. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 of the dietary supplement composition of the present invention.

In addition to the above, health food and health functional food composition containing the active substance of the present invention are various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health food and health functional food composition of the present invention may contain natural fruit juice, fruit juice, and fruit for the production of fruit juice drinks and vegetable drinks.

These components may be used independently or in combination. The proportion of these additives is not so important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health food and health functional food composition containing the active substance of the present invention.

pharmaceutical composition

One aspect of the present invention also provides a pharmaceutical composition for the prevention or treatment of muscle loss-related diseases comprising a mixed extract of upper extremity, cephalosporidium, oleracea and black bean.

In one embodiment of the present invention, the mixed extract of the upper extremity, cephalothorax, serrata and black bean may be the same as the mixed extract of the above aspect, and the description of the mixed extract is replaced by the description of the above aspect.

The active substance of the present invention can be administered in various oral and parenteral dosage forms during clinical administration, and when formulating, commonly used fillers, extenders, binders, wetting agents, disintegrants, and diluents or excipients such as surfactants are used. is manufactured by

Solid preparations for oral administration include tablets, patients, powders, granules, capsules, troches, etc., and these solid preparations include at least one or more excipients, for example, starch, calcium carbonate, It is prepared by mixing sucrose, lactose, or gelatin. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Liquid formulations for oral administration include suspensions, solutions, emulsions, or syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. can

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspension solutions, emulsions, lyophilized formulations, suppositories, and the like. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerol, gelatin, and the like can be used.

In addition, the effective dosage for the human body of the active substance of the present invention may vary depending on the patient's age, weight, sex, dosage form, health status and disease degree, and is generally about 0.001-100 mg/kg/day, Preferably it is 0.01-35 mg/kg/day. Based on an adult patient weighing 70 kg, it is generally 0.07-7000 mg/day, preferably 0.7-2500 mg/day, and once a day at regular time intervals according to the judgment of a doctor or pharmacist It may be administered in several divided doses.

The present invention will be described in more detail through the following examples. However, the following examples are only intended to embody the contents of the present invention, and the present invention is not limited thereto.

Example

Example 1. Preparation of mixed extract

2 Kg of upper extremities and 0.4 kg of each of the upper extremities, scallops, cephalothorax and black bean were mixed in a weight ratio of 5: 1: 1: 1, added 100 kg of water, heated at 110 ° C for 4 hours, cooled naturally to 40 ° C, and filtered. Mixed extracts of upper extremities, scapulae, duchung and black bean were prepared.

Comparative Example 1. Preparation of upper extremity extract

In Example 1, the upper extremity extract was prepared in the same manner as in Example 1, except that the upper extremities were used as a single material instead of the upper extremities, the scorpion spp.

Comparative Example 2. Preparation of extract

In Example 1, the extract was prepared in the same manner as in Example 1, except that, in place of the upper extremities, scorpion hornwort, cephalothorax, and black bean, oxalis spp. was used as a single material.

Comparative Example 3. Preparation of Duchung Extract

In Example 1, an extract was prepared in the same manner as in Example 1, except that Duchung was used as a single material instead of upper extremity, scapulae, Duchung and Heukdu.

Comparative Example 4. Preparation of black bean extract

Heuk bean extract was prepared in the same manner as in Example 1, except that in Example 1, black bean was used as a single material instead of upper extremity, scapulae, duchung and heuk bean.

Comparative Example 5. Preparation of mixed extracts of oleracea, duchung and black bean

In Example 1, the same method as in Example 1 was carried out, except that, instead of upper extremity, scorpion spp., and black bean, a mixed material was used as a mixed material of 1: 1: 1 by weight ratio, , Duchung and Heukbean mixed extracts were prepared.

Reference Example 1. Preparation of experimental materials

C2C12 cells were purchased from ATCC and the mixture was diluted in water.

Reference Example 2. Cell culture

C2C12 cells were cultured in a DMEM medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin as a culture medium at 37 °C, CO ₂ incubator. During differentiation, 2% HS (horse serum) was used instead of 10% FBS. Incubated for 6 days at 37 °C, 5% CO ₂ conditions.

Reference example 3. Statistical processing

The statistical significance of the experimental results was expressed as the mean ± standard deviation, and the difference between each group was analyzed for significance by using the SPSS 25 (SPSS Inc. USA) program, and by performing post-hoc tests using ANOVA and Duncan test. Significance was recognized when P<0.05 or less, and statistical significance with the normal group was indicated in the drawing. Each experiment was performed at least 3 times for statistical processing.

Experimental Example 1. Evaluation of cell viability

Cell viability evaluation of the sample for C2C12 was measured using MTT (thiazoly blue tetrazolium bromid). That is, the C2C12 cells were seeded in a 96-well plate at a concentration of 1×106 cells/mL the day before the experiment, and the samples (extracts) obtained in Example 1 and Comparative Examples 1 to 4 were applied to 0.1 mg/mL to 1 mg/mL. treated and incubated for 24 hours.

After that, MTT stored at 4 ℃ is dissolved in PBS at a concentration of 1 mg/mL, and then, using a pipet, take an amount equal to 20% of the 96-well medium volume, carefully add it to each well, and attach the plate to the bottom. It was mixed by gently shaking it back and forth with a left and right side.

After culturing in a CO ₂ incubator for 4 hours, the culture medium is removed, formazan is eluted by adding 200 μL of DMSO, and the eluted solution is transferred to a new 96-well plate and measured at the absorbance value at 570 nm using ELISA. is shown in Table 1 and Figure 1 below:

[Table 1]

Referring to Table 1 and FIG. 1, when each extract was treated from 0.1 mg/mL to 1 mg/mL by concentration, the upper extremity extract of Comparative Example 1 and the Duchong extract of Comparative Example 2 were each 68.28± at a concentration of 0.6 mg/mL. Cytotoxicity was shown at 2.03%, 81.93±2.01%, and the extract of Comparative Example 3 showed cytotoxicity at a concentration of 0.8 mg/mL at 45.03±7.28%, whereas in the mixed extract of Example 1, the cells were cytotoxic at all concentrations. It was confirmed that there was no toxicity.

Experimental Example 2. H ₂ O ₂ Evaluation of the protective effect of cell damage caused by oxidative stress according to treatment

MTT assay was used to confirm the protective effect on cell damage caused by oxidative stress according to H ₂ O ₂ treatment. To this end, myoblasts of C2Cl2 cells and myotubes differentiated therefrom were dispensed at 1×106 cells/well, respectively, in a 6 well plate for cell culture, and after stabilizing for 48 hours, the mixed extract of Example 1 And the extracts of Comparative Examples 1 to 5 were pre-treated at a concentration of 0.1 mg/mL to 0.4 mg/mL for 2 hours, followed by H ₂ O ₂ . After incubation for 24 hours, the MTT reagent was diluted to a concentration of 1 mg/mL and treated, and then reacted again at 37° C. for 4 hours. After the reaction was completed, the MTT reagent was removed, and all formazin generated in each well was dissolved using DMSO. Then, 200 μL was transferred to a 96 well plate, and the absorbance value at 570 nm was measured using ELISA.

In myoblast, cell viability values for each concentration due to the treatment of the mixed extract of Example 1 are shown in a) of FIG. 2, when each of the extracts of Comparative Examples 1 to 5 and Example 1 were treated with 0.1 mg/mL The measured values of cell viability are shown in Table 2 below and in FIG. 2 b):

[Table 2]

Referring to FIG. 2 a), when Myoblast was treated with H ₂ O ₂ 0.8 mM, the viability was measured to be 47.24±4.25% compared to the control (CON), and the mixed extract of Example 1 0.1, 0.2 and 0.4 mg/ It was confirmed that the decreased survival rate when treated with H ₂ O ₂ alone at 78.43±2.22, 78.11±1.81, and 92.75±16.66 %, respectively, when treated with mL concentration, blocks the decrease in survival rate due to oxidative stress in a concentration-dependent manner during the mixed extract treatment. could

In addition, referring to Table 2 and b) of FIG. 2, at a concentration of 0.1 mg/mL of each extract, the upper extremity of Comparative Example 1, Duchung of Comparative Example 2, S. oleraceae of Comparative Example 3, Heukbean single extract of Comparative Example 4 and Compared with the mixed extracts of Duchung, Ogapi, and Heukpea of Comparative Example 5, the best cell protective activity was shown in the mixed extract of upper extremity, Duchung, Ogapi, and Heukpea of Example 1, and thus it was confirmed that there was a synergistic effect of each extract.

In Myotube, the cell viability value for each concentration due to the treatment of the mixed extract of Example 1 is shown in a) of FIG. 3, when each of the extracts of Comparative Examples 1 to 5 and Example 1 were treated with 0.1 mg/mL The measured values of cell viability are shown in Table 3 and Figure 3b) below:

[Table 3]

Referring to FIG. 3 a), when Myotube is treated with H ₂ O ₂ 0.8 mM, the viability of 65.98±1.25% compared to the control (CON) was measured, and the mixed extract of Example 1 0.1, 0.2 and 0.4 mg/ When treated with mL concentration, the reduced survival rate when treated with H ₂ O ₂ alone at 127.46±9.76, 103.48±2.09 and 99.67±3.05%, respectively, was not concentration-dependent when treated with the mixed extract, but it was found that it blocks the decrease in survival rate due to oxidative stress. Confirmed.

In addition, referring to Table 3 and b) of FIG. 3 , at a concentration of 0.1 mg/mL of each extract, the upper extremity of Comparative Example 1, the cephalothorax of Comparative Example 2, the oleander of Comparative Example 3, and the single black bean extract of Comparative Example 4 and Compared with the mixed extracts of Duchung, Ogapi, and Heukpea of Comparative Example 5, the best cell protective activity was shown in the mixed extract of upper extremity, Duchung, Ogapi, and Heukpea of Example 1, and thus it was confirmed that there was a synergistic effect of each extract.

Manufacturing example of health food

The active substance according to the present invention can be manufactured into various types of health food depending on the purpose. The following exemplifies the manufacturing method of several health foods containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health food production example 1> Production of dairy products

0.01-1 parts by weight of the active substance of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health food production example 2> Preparation of wire

Brown rice, barley, glutinous rice, and barley radish were pregelatinized by a known method and dried, and then roasted and prepared as a powder having a particle size of 60 mesh with a grinder. Black soybeans, black sesame, and perilla were also steamed and dried by a known method, and then roasted and prepared into powder having a particle size of 60 mesh with a grinder. The active material of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. It was prepared by mixing the dry powders of grains, seeds and active substances prepared above in the following ratios.

Grains (34 parts by weight of brown rice, 19 parts by weight of barley, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame),

Active substance (2 parts by weight),

Reishi (1.5 parts by weight), and

Rehmannia (1.5 parts by weight).

Manufacturing example of health functional food

The active substance according to the present invention can be manufactured into various types of health functional food depending on the purpose. The following exemplifies the manufacturing method of several health functional foods containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health functional food manufacturing example 1> Manufacture of health functional food

100 mg of active substance

appropriate amount of vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

potassium phosphate monobasic 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food in a preferred embodiment, the composition ratio may be arbitrarily modified. Then, the granules can be prepared and used in the production of a health functional food composition according to a conventional method.

<Health functional food production example 2> Preparation of health functional beverage

100 mg of active substance

100 mg citric acid

100 mg of oligosaccharides

Plum Concentrate 2 mg

Taurine 100 mg

Add purified water to total 500 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, after stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized container, sealed and sterilized, and then refrigerated. used in the manufacture of health beverage compositions. Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demand country, and use purpose.

Preparation example of drug

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following exemplifies some formulation methods containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Pharmaceutical Preparation Example 1> Preparation of powder

2 g of active substance

1 g lactose

After mixing the above ingredients, the powder was prepared by filling in an airtight cloth.

<Pharmaceutical Preparation Example 2> Preparation of tablets

Active substance 100 mg

Corn Starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, tablets were prepared by tableting according to a conventional manufacturing method of tablets.

<Pharmaceutical Preparation Example 3> Preparation of capsules

Active substance 100 mg

Corn Starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, the capsules were prepared by filling in gelatin capsules according to a conventional manufacturing method of capsules.

<Pharmaceutical Preparation Example 4> Preparation of injection

Active substance 10 μg/ml

Dilute hydrochloric acid BP until pH 3.5

Sodium Chloride BP for Injection up to 1 ml

The active substance according to the present invention was dissolved in an appropriate volume of sodium chloride BP for injection, the pH of the resulting solution was adjusted to pH 3.5 using dilute hydrochloric acid BP, the volume was adjusted using sodium chloride BP for injection, and the mixture was sufficiently mixed. . The solution was filled in a 5 ml Type I ampoule made of clear glass, sealed under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120° C. for 15 minutes or more to prepare an injection solution.

<Pharmaceutical Preparation Example 5> Preparation of nasal absorbent (Nasal spray)

Active substance 1.0 g

0.3 g sodium acetate

Methylparaben 0.1 g

Propylparaben 0.02 g

Sodium Chloride Appropriate amount

HCl or NaOH pH adjustment appropriate amount

Purified water appropriate amount

According to a conventional method for preparing nasal absorbents, prepare to contain 3 mg of active substance per 1 mL of saline (0.9% NaCl, w/v, solvent is purified water), fill it in an opaque spray container, and sterilize to prepare nasal absorbent prepared.

<Pharmaceutical Preparation Example 6> Preparation of liquid preparation

100 mg of active substance

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each ingredient is added to purified water to dissolve, lemon flavor is added, the above ingredients are mixed, then purified water is added to adjust the total to 100 mL, filled in a brown bottle and sterilized to prepare a liquid preparation did

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (12)

A health food composition for preventing or improving muscle loss-related diseases caused by oxidative stress, comprising a mixed extract of upper extremities, cephalothorax, oleander and black bean.
The method of claim 1,
The health food composition, characterized in that 10 to 30 parts by weight of the tofu, 10 to 30 parts by weight, and 10 to 30 parts by weight of the black bean, based on 100 parts by weight of the upper extremity.
3. The method of claim 2,
The health food composition, characterized in that 17 to 23 parts by weight of the tofu, 17 to 23 parts by weight of the ogapi, and 17 to 23 parts by weight of the black bean, based on 100 parts by weight of the upper extremity.
4. The method of claim 3,
Based on 100 parts by weight of the upper extremity, 19 to 21 parts by weight of the duchung, 19 to 21 parts by weight of the ogapi, and 19 to 21 parts by weight of the black bean are included.
The method of claim 1,
The mixed extract is a health food composition, characterized in that the mixture is extracted with water, a straight or branched chain alcohol having 2 to 4 carbon atoms, or a mixture thereof.
6. The method of claim 5,
The mixed extract is a health food composition, characterized in that extracted with water.
The method of claim 1,
The muscle loss-related disease is a health food composition, characterized in that at least one selected from the group consisting of sarcopenia, muscular atrophy, muscular dystrophy, atonia and myasthenia gravis.
delete The method of claim 1,
The health food is a health food composition, characterized in that it is selected from various drinks, meat, sausage, bread, candy, snacks, noodles, ice cream, dairy products, soup, beverages, alcoholic beverages, gum, tea and vitamin complexes.
A health functional food composition for preventing or improving muscle loss-related diseases caused by oxidative stress, comprising a mixed extract of upper extremities, cephalothorax, oleander and black bean.
delete It contains a mixed extract of upper extremity, cephalosporus, oleracea, and black bean,
A pharmaceutical composition for the prevention or treatment of one or more muscle loss-related diseases selected from the group consisting of sarcopenia, muscular atrophy, muscular dystrophy, atonia and myasthenia gravis induced by oxidative stress.

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