KR102302047B1 - Composition for hepatoprotective and ameliorating hangover - Google Patents

Abstract

The present invention relates to a composition for liver protection or hangover relief, comprising: a mixed extract extracted from a mixture consisting of milk thistle and dandelion; pear juice; aronia juice; And honey; by containing a mixture consisting of an active ingredient, it is effective in relieving a hangover and protecting the liver, and since it is non-toxic, it can be consumed in the form of food. It can improve, prevent or treat liver disease.

Description

Thistle, dandelion, pear, aronia and a composition for hepatoprotective or hangover containing a mixture consisting of honey {Composition for hepatoprotective and ameliorating hangover}

The present invention relates to a composition for protecting the liver or relieving a hangover containing a mixture consisting of a mixture of milk thistle and dandelion extract, pear juice, aronia juice, and honey as active ingredients.

Alcohol, when consumed in moderation, promotes metabolism and is a means of relieving stress, but excessive intake of alcohol can cause thirst, general malaise, fatigue, memory loss, bloating, indigestion, vomiting, diarrhea, and vitamin deficiency. suffer from hangovers. Also, alcohol dilates the blood and can cause microvascular rupture. Therefore, excessive alcohol consumption causes thin blood vessels to appear on the face and body. Also, as it removes moisture from the cellular tissue, keratinization occurs and the skin becomes rough with neutral or oily skin. In addition, usually the next day after drinking too much, the face or eyes are swollen and pimples appear.

This hangover phenomenon is known to be caused by the action of alcohol and acetaldehyde accumulated in liver cells and the body. Ingested ethanol is absorbed through the digestive tract, reaching peak blood levels between 20 and 120 minutes after ingestion. Absorbed ethanol is metabolized in all organs including the liver, some excreted through respiration, urine or sweat.

Ethanol entering the liver is converted to acetate by the action of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cytoplasm, which is excreted out of the liver cells through the circulation. That is, alcohol is oxidized to acetaldehyde by alcohol dehydrogenase (ADH) and coenzyme NAD+ in the liver, and acetaldehyde is decomposed into acetic acid by aldehyde dehydrogenase (ALDH) and NAD+. It is also oxidized to acetaldehyde by cytochrome P-450 type 2E1 (CYP2E1) and catalase. Acetaldehyde and acetic acid are known to cause cytotoxicity, headache or abdominal pain, and dehydration due to changes in osmotic pressure through lipid peroxidation. have.

Among liver diseases, diseases caused by chronic drinking are largely divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis, but it is rare that only one pure disease appears in one person, and each disease appears to varying degrees for each individual. It is the most common cause of cirrhosis in the United States and the second most common cause of cirrhosis in Korea after viral hepatitis.

As a prior art for a composition for liver protection, Korean Patent Registration No. 10-0477957 (a novel pyrrole derivative having a liver-protective activity isolated from Goji berry and a composition containing the same), Republic of Korea Patent No. 10-0633851 (heat-dried process) There is a composition for liver protection or prevention and treatment of liver disease containing wild garlic extract as an active ingredient), and Korean Patent Registration No. 10-1106499 (food composition for liver protection effect containing extract of Heotgae sprigs) and the like.

In addition, for hangover remedies, Korean Patent Laid-Open No. 2002-0081995 (Hangover Relief and Liver Function Improving Agent by Formulated Food and Manufacturing Method), Republic of Korea Patent Publication No. 2002-0064151 (Hepatotoxicity and hangover relieving activity isolated from H. Although it has been reported that lower alcohol insoluble extract fractions and polysaccharide substances with have.

Therefore, in the direction of the development of functional food related to liver health, it is advantageous to develop a complex formulation of natural products that have effects on each factor that causes liver disease, rather than using a single natural product that helps maintain liver health as a raw material. For this purpose, it can be said that it is necessary to evaluate the activity of each liver disease factor by targeting many natural products.

Republic of Korea Patent Registration No. 10-0477957 Republic of Korea Patent Publication No. 2002-0081995

An object of the present invention is a mixed extract obtained by extracting a mixture consisting of thistle and dandelion; pear juice; aronia juice; and honey; to provide a composition for liver protection or hangover relief containing a mixture consisting of an active ingredient.

In addition, another object of the present invention is a mixed extract obtained by extracting a mixture consisting of thistle and dandelion; pear juice; aronia juice; And honey; to provide a pharmaceutical composition for the prevention or treatment of alcoholic liver disease containing a mixture consisting of an active ingredient.

In addition, another object of the present invention is a mixed extract obtained by extracting a mixture consisting of thistle and dandelion; pear juice; aronia juice; And honey; to provide a food composition for the prevention or improvement of alcoholic liver disease containing a mixture consisting of an active ingredient.

In addition, another object of the present invention is to provide a method for preparing a composition for the liver protection or hangover relief.

The composition for liver protection or hangover relief of the present invention for achieving the above object is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; pear juice; aronia juice; and honey; may contain a mixture consisting of an active ingredient.

The mixed extract may be an extract extracted at 80 to 100° C. by mixing milk thistle and dandelion in a weight ratio of 1: 0.2-1.

Based on 100 parts by weight of the mixed extract, 1 to 30 parts by weight of pear juice, 1 to 20 parts by weight of aronia juice, and 0.1 to 5 parts by weight of honey may be mixed.

The thistle may be mixed with a mixture of flowers, stems and leaves, and roots of thistles in a weight ratio of 1:14-25:0.1-0.9.

In addition, the pharmaceutical composition for the prevention or treatment of alcoholic liver disease of the present invention for achieving the above other object is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; pear juice; aronia juice; and honey; may contain a mixture consisting of an active ingredient.

The alcoholic liver disease may be alcoholic hepatitis, alcoholic fatty liver or alcoholic cirrhosis.

In addition, the food composition for preventing or improving alcoholic liver disease of the present invention for achieving the above another object is a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; pear juice; aronia juice; and honey; may contain a mixture consisting of an active ingredient.

In addition, the method for preparing a composition for liver protection or hangover relief of the present invention for achieving the above another object comprises the steps of (A) obtaining a mixed extract by mixing and extracting milk thistle and dandelion; (B) mixing by adding pear juice and aronia juice to the mixed extract; and (C) mixing by adding honey and stevia to the mixture in which the pear juice and aronia juice are mixed.

Hepatoprotective or hangover relief composition of the present invention; Or the composition for the prevention, treatment or improvement of alcoholic liver disease promotes the decomposition of alcohol and acetaldehyde in the blood, and relieves hangover that increases the activity of aldehyde dehydrogenase; And alcoholic hepatitis, alcoholic fatty liver or alcoholic liver disease, such as alcoholic liver cirrhosis, can be prevented, treated or improved, and can be consumed in the form of food because it is non-toxic.

The present invention relates to a composition for protecting the liver or relieving a hangover containing a mixture consisting of a mixture extracted from a mixture consisting of milk thistle and dandelion, pear juice, aronia juice, and honey as active ingredients.

Hereinafter, the present invention will be described in detail.

Hepatoprotective or hangover relief composition of the present invention; Alternatively, the composition for the prevention, treatment or improvement of alcoholic liver disease contains a mixture consisting of a mixture extracted from a mixture consisting of milk thistle and dandelion, pear juice, aronia juice and honey as an active ingredient. In addition, stevia and agar may be added in addition to the active ingredients.

thistle

The thistle used in the present invention may be a white thistle (Silybum marianum (L.) Gaertn.), a spiny thistle (Cirsium japonicum Fisch ex DC. var. spinossimum Kitam.), or a mixture thereof, preferably a white thistle. Thistle alone is used.

The white patterned thistle (Silybum marianum (L.) Gaertn.) is a deep, shiny green plant with milky white leaf veins, and a wavy edge at the edge and is a thorny root-leaf divided into long thin plants. It is mainly found on levee fences and abandoned lands. The useful parts of the plant are the whole herb, roots, leaves, seeds, and husks, and thistle seeds contain a flavonoid compound known as silymarin. Thistle seed extract contains 70-80% silymarin.

The thistle according to the present invention is a mixed extract containing a mixture of flowers, stems and leaves, and roots of thistle.

The flower of the thistle has a purple or red corolla and has a length of 19-24 mm, and it is preferable to use only those with a length of 10 to 15 mm and not use those with a longer length than that for toxicity.

In addition, the thistle stem and leaves are used by mixing in a weight ratio of 1: 1-4, preferably 1: 2-3. If the content of the leaf based on the stem is less than the lower limit, the bitter taste may be strong, and if it exceeds the upper limit, it is not preferable for relieving a hangover and preventing, treating or improving alcoholic liver disease.

In addition, unlike flowers, stems and leaves, which are harvested twice a year, the thistle root is harvested once every three years, so it is preferable to use it after drying.

The flower, stem and leaf mixture, and root of the thistle are mixed in a weight ratio of 1:14-25:0.1-0.9, preferably 1:16-19:0.4-0.8 by weight. If the content of the mixture of stems and leaves and roots based on the flower is less than the lower limit, the effect on relieving a hangover and the prevention, treatment or improvement of alcoholic liver disease may not be excellent. It is strong and the effect on the prevention, treatment or improvement of hangover relieving and alcoholic liver disease may be reduced.

The mixed extract of the present invention is a hot water extract extracted at 80 to 100 °C for 20 to 30 hours, preferably for 24 to 28 hours. If the extraction temperature and time are less than the lower limit, the active ingredient may not be extracted, and if the extraction temperature and time are more than the upper limit, the burnt taste may be strong and the nutrients may be destroyed. In addition, when extraction is performed by other methods, such as organic solvent extraction, in addition to water bath extraction, the effect on relieving a hangover and prevention, treatment or improvement of alcoholic liver disease is insignificant, and a strong bitter taste may occur.

Specifically, the mixed extract is extracted by mixing flowers, and a mixture of stems and leaves, and separately extracting the roots in a bath and mixing them afterwards. Unlike the present invention, in the case of extracting the hot water by mixing all of the mixture and roots of flowers, stems and leaves, the bitter taste is strong, and the effect of relieving a hangover and preventing, treating or improving alcoholic liver disease may be reduced.

Specifically, the method for preparing such a thistle mixed extract includes the steps of: (a) mixing a mixture of flowers, stems and leaves of thistle, and extracting the extract with hot water at 80 to 100°C; (b) extracting the root of thistle at 80 to 100° C. to obtain an extract; and (c) lyophilizing the extracts obtained in steps (a) and (b), respectively, to powder them and mixing them. In addition, after step (c), (d) drying and pulverizing the residue remaining after extraction in step (b); and (e) mixing 100 parts by weight of the powder mixed in step (c) and 0.1 to 3 parts by weight of the powder of step (d); may further include.

Unlike the steps (a) and (b), when extracting by mixing all of the flowers, stems and leaves, and roots, the bitter taste is strong and, rather, the effect of relieving hangover and preventing, treating or improving alcoholic liver disease may be lowered; In the case of extracting flowers, a mixture of stems and leaves, and roots, respectively, in a bath, the yield may be reduced.

When the root residue powder prepared in step (d) is added, the effect on disease can be significantly improved compared to the case where the root residue powder is not added.

dandelion

The dandelion (Taraxacum platycarpum) is also called Geumjamcho, Jijeong, Pogongyeong, Pogongcho, Anjilbang, and also has a nickname called Satseunbang. The leaves or stems contain proteins, carbohydrates, fats, ash, minerals, vitamins, etc. evenly. Young shoots are used as greens or soup. In oriental medicine, the roots and the outpost before flowering are said to have antipyretic, anti-inflammatory, diuretic, and gastric effects, so it is used as a treatment for hypothyroidism, sore throat, bronchitis, lymphadenitis, ocular disease, mastitis, hepatitis, cholecystitis, indigestion, indigestion, and constipation. use it

In the present invention, milk thistle and dandelion are mixed in a weight ratio of 1:0.2-1, preferably 1:0.4-0.6.

If the content of dandelion based on milk thistle is less than the above lower limit, the effect of relieving hangover and preventing, treating or improving alcoholic liver disease may be lower than when each substance is used alone, and if it exceeds the upper limit, hangover relief and may have little effect on the prevention, treatment or improvement of alcoholic liver disease.

The mixed extract mixed with thistle and dandelion of the present invention is obtained by mixing a mixture of thistle and dandelion and an extraction solvent in a weight ratio of 1:1 to 100, preferably 1: 5 to 20, and then 40 to 50 at 80 to 100 ° C. Extract over time to prepare an extract. When the weight ratio of the mixture and the extraction solvent is out of the above range, the active ingredient of the milk thistle and dandelion mixture may be extracted in a small amount in the extract.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The extraction solvent is not particularly limited, but the extract extracted with water shows an excellent effect.

pear juice

The pear juice is sterilized at 90 to 110 ° C. for 3 to 10 minutes by squeezing a fruit, a pear.

When used alone, the pear juice has little effect in relieving a hangover and preventing, treating or improving alcoholic liver disease, but when used with the milk thistle and dandelion mixed extract and aronia juice, pear juice alone, the mixed extract alone or aronia It shows 1.5 to 20 times superior efficacy compared to the case of using the juice alone.

The pear juice is used in an amount of 1 to 30 parts by weight, preferably 5 to 20 parts by weight, based on 100 parts by weight of the mixed extract. If the content of pear juice is less than the lower limit, there may be no effect of relieving a hangover and preventing, treating or improving alcoholic liver disease.

aronia

The aronia juice is sterilized at 90 to 110° C. for 3 to 10 minutes by squeezing aronia.

When used alone, the aronia juice has little effect in relieving a hangover and preventing, treating or improving alcoholic liver disease, but when used with the milk thistle and dandelion mixed extract, aronia juice alone or the mixed extract alone It shows 1.5 to 20 times superior efficacy compared to the case.

The aronia juice is used in an amount of 1 to 20 parts by weight, preferably 1 to 10 parts by weight, based on 100 parts by weight of the mixed extract. If the content of aronia juice is less than the lower limit, there may be no effect of relieving a hangover and preventing, treating, or improving alcoholic liver disease, and if it exceeds the upper limit, the sensuality may be reduced.

honey

When used alone, the honey is effective only in relieving a hangover, but when used in combination with the milk thistle and dandelion mixed extract, pear juice and aronia juice, it is effective not only in relieving a hangover, but also in preventing, treating or improving alcoholic liver disease.

The honey is used in an amount of 0.1 to 5 parts by weight, preferably 0.5 to 3 parts by weight, based on 100 parts by weight of the mixed extract. If the content of honey is less than the lower limit, there may be no effect of relieving a hangover and preventing, treating or improving alcoholic liver disease, and if it exceeds the upper limit, the sensuality may be reduced and the manufacturing cost may be improved.

Stevia

The stevia ( Stevia rebaudiana ) grows around rivers or wetlands, and has been using stevia leaves as a sweetener since ancient times. The leaves contain stevioside, a sweetening substance, about 6-7% by weight, and the content varies greatly depending on the individual. The sweetening ingredient is 300 times that of sugar, and it is used as a sweetener in soft drinks, as a substitute for making tea or chewing gum.

In the present invention, by using stevia, it is effective in preventing, treating or improving not only stevia, but also hangover relieving and alcoholic liver disease when used together with milk thistle and dandelion.

The stevia is mixed in an amount of 0.1 to 5 parts by weight, preferably 0.2 to 1 parts by weight, based on 100 parts by weight of milk thistle.

When the content of stevia is less than the lower limit, the effect of relieving hangover and preventing, treating or improving alcoholic liver disease may be lower than when each substance is used alone. And the effect of preventing, treating or ameliorating alcoholic liver disease may be reduced.

In addition, the present invention is a composition for liver protection or hangover relief; Or it provides a method for preparing a composition for preventing, treating or improving alcoholic liver disease.

The method of preparing a composition for relieving hangover and preventing, treating or improving alcoholic liver disease of the present invention comprises the steps of (A) obtaining a mixed extract by mixing and extracting milk thistle and dandelion; (B) mixing by adding pear juice and aronia juice to the mixed extract; and (C) mixing by adding honey and stevia to the mixture in which the pear juice and aronia juice are mixed.

First, in step (A), a mixture of milk thistle and dandelion is extracted with an extraction solvent to obtain a mixed extract.

The mixing conditions, extraction solvent and extraction conditions of the mixture are the same as those mentioned above.

Next, in step (B), pear juice and aronia juice are added to the mixed extract at room temperature (23-27° C.) to prepare a mixture of pear juice and aronia juice.

When preparing a mixed extract by mixing milk thistle and dandelion in step (A), instead of adding pear juice and aronia juice to extract, pear juice and aronia juice are added separately later to increase sensibility and relieve hangover and alcoholic liver disease It is possible to further improve the preventive, therapeutic or ameliorating effect of When extraction is performed by adding pear juice and aronia juice to milk thistle and dandelion, the effect of relieving hangover and preventing, treating, or improving alcoholic liver disease may be insignificant. It is preferable to add

Next, in step (C), honey and stevia are added to the mixture in which the pear juice and aronia juice are mixed at room temperature and mixed.

The honey and stevia are added after the pear juice and aronia juice are sufficiently mixed with the mixed extract, and when the pear juice and aronia juice and honey are added together, the organoleptic properties may be significantly reduced.

The form of the composition of the present invention may be prepared in the form of gel, puree, juice, pills, large pills and granules.

For example, when it is prepared in the form of gel and puree, the mixture of pear juice and aronia juice prepared in step (B) is concentrated to 1/3 to 1/5 of the volume of the mixture with a concentrator. Then, step (C) is performed using the concentrate. That is, after mixing the concentrate, honey, and stevia, agar is added, stirred, and then sterilized to prepare gel and puree.

As another example, when it is prepared in the form of juice, the mixture prepared in step (C) is sterilized and used as it is.

As another example, when it is prepared in the form of a ring, a large ring and a granule, the mixture in which the pear juice and aronia juice prepared in step (B) are mixed is used with a concentrator in a volume of 1/5 to 1/7 compared to the volume of the mixture After concentrating with , honey and stevia are added to the concentrate, aged for 40 to 50 hours in a water bath at 60 to 70 ° C., and then dried and molded to prepare pills, large rings and granules.

the mixed extract of the present invention; pear juice; aronia juice; and honey; a mixture consisting of a mixed extract in a broad sense; pear juice; aronia juice; and honey; a mixed product formulated to be administered to an animal, such as a mixed extract; pear juice; aronia juice; and honey mixed powder. Although in the present invention the mixed extract; pear juice; aronia juice; and honey; although the experiment was conducted with a mixture consisting of, a mixed extract; pear juice; aronia juice; And it will be expected by those skilled in the art that the desired effect can be achieved even in the form of a mixed processed product of honey.

On the other hand, in the present specification, the term 'contained as an active ingredient' means a mixed extract; pear juice; aronia juice; and honey; is meant to include an amount sufficient to achieve the efficacy or activity of the mixture consisting of For example, the mixed extract; pear juice; aronia juice; and honey; is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. mixed extract; pear juice; aronia juice; And honey as a natural product, even when administered in excess, there is no side effect on the human body, so the mixed extract contained in the composition of the present invention; pear juice; aronia juice; And the quantitative upper limit of honey can be carried out by selecting within an appropriate range by those skilled in the art.

The pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, a disintegrant, a sweetener, a binder, a coating agent, a swelling agent, a lubricant, A lubricant or flavoring agent may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectables. For formulation in the form of, for example, tablets or capsules, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.

Furthermore, by using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the art, it can be preferably formulated according to each disease or component.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration. .

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration mode, age, weight, sex, morbidity, food, administration time, administration route, excretion rate and response sensitivity of the patient, An ordinarily skilled physician can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dose of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person of ordinary skill in the art to which the present invention pertains. or may be prepared by incorporation into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion in oil or an aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or stabilizer.

In addition, the present invention is a mixed extract; pear juice; aronia juice; And honey; provides a food composition for the prevention, treatment or improvement of hangover relieving and alcoholic liver disease containing a mixture consisting of an active ingredient.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, sweets, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice cream, vitamin complexes, health supplements etc.

The food composition of the present invention is a mixed extract as an active ingredient; pear juice; aronia juice; and honey, as well as ingredients commonly added during food production, including, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink and beverages, the mixed extract of the present invention; pear juice; And in addition to honey, citric acid, fructose liquid, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be further included.

The present invention is the mixed extract; pear juice; aronia juice; And it provides a health functional food comprising a food composition for the prevention, treatment or improvement of hangover relief and alcoholic liver disease containing honey as an active ingredient. Health functional food is a mixed extract; pear juice; aronia juice; And honey is added to food materials such as beverages, teas, spices, gum, and confectionery, or is a food manufactured by encapsulation, powdering, suspension, etc. Contrary to this, it has the advantage that there are no side effects that may occur when taking the drug for a long time because it uses food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. Mixed extracts in such health functional foods; pear juice; aronia juice; And the amount of honey added varies depending on the type of health functional food subject to it and cannot be defined uniformly, but it may be added within a range that does not impair the original taste of the food, and is usually 0.01 to 50% by weight relative to the target food, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention is a mixed extract for the manufacture of drugs or food for the prevention, treatment or improvement of hangover relieving and alcoholic liver disease; pear juice; aronia juice; and honey use. mixed extracts as described above; pear juice; aronia juice; And honey can be used for hangover relief and alcoholic liver disease.

In addition, the present invention provides an effective amount of a mixed extract for a mammal; pear juice; aronia juice; And it provides a method of relieving a hangover and improving, preventing or treating alcoholic liver disease, comprising administering a mixture of honey.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term “effective amount” refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as conceived by a researcher, veterinarian, physician or other clinician, which an amount that induces amelioration of the symptoms of a disease or disorder. It is apparent to those skilled in the art that the effective amount and frequency of administration for the active ingredient of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of the active ingredient and other components contained in the composition, the type of formulation, and the age, weight, and general health of the patient It can be adjusted according to various factors including condition, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and concurrently used drugs. In the prevention, treatment or improvement method of the present invention, for adults, a mixed extract; pear juice; aronia juice; and honey; when the mixture is administered once to several times a day, it is preferably administered in a dose of 0.001 g/kg to 10 g/kg.

Mixed extract in the treatment method of the present invention; pear juice; aronia juice; and honey; as an active ingredient, a composition comprising a mixture consisting of oral, rectal, intravenous, intra-arterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes to be administered in a conventional manner. can

Hereinafter, preferred examples are presented to aid the understanding of the present invention, but the following examples are merely illustrative of the present invention and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1.

production of thistle

1 part by weight of the flower of thistle, 18.6 parts by weight of a mixture of stem and leaf (stem:leaf=1:2 weight ratio) and 45 parts by weight of water were mixed and extracted at 95° C. for 24 hours to obtain an extract A, followed by 0.4 parts by weight of thistle root. Extract B was obtained by extracting parts by weight and 45 parts by weight of water at 95° C. for 24 hours, and then lyophilized to prepare powder A and powder B, respectively, and mixed to prepare a mixed extract powder of thistle.

Preparation of mixtures

After mixing the prepared milk thistle and dandelion in a weight ratio of 1:0.42, the mixture and water were mixed in a weight ratio of 1:9 and extracted at 90° C. for 48 hours to prepare a mixed extract. Add 6.8 parts by weight of sterilized pear juice and 4.5 parts by weight of sterilized aronia juice to 100 parts by weight of the mixed extract and mix at room temperature for 10 minutes, then add 1.0 parts by weight of honey and 0.2 parts by weight of stevia and mix at room temperature for 10 minutes Thus, a mixture consisting of a mixed extract, pear juice, aronia juice, honey and stevia was obtained.

Example 2. Addition of Root Residue of Thistle

In the same manner as in Example 1, except that when the milk thistle powder was prepared, the thistle root was extracted with hot water and the remaining residue was dried and pulverized to obtain powder C (root residue powder), and then powder A, powder B and powder C were prepared. Mixing (0.3 parts by weight of powder C relative to 100 parts by weight of powder A + powder B) was performed to prepare a mixed extract powder of thistle, and then a composition was prepared using this.

Comparative Example 1. Using only the mixed extract

It was carried out in the same manner as in Example 1, except that only the mixed extract was used without using pear juice, aronia juice, honey and stevia.

Comparative Example 2. Different ratio of milk thistle

1 part by weight of the flower of thistle and 11 parts by weight of a mixture of stem and leaf (stem:leaf=1:2 weight ratio) were mixed and extracted in hot water at 95°C for 24 hours to obtain an extract A, and 1.0 parts by weight of thistle root at 95°C Extract B was obtained by extraction with hot water for 24 hours, and then lyophilized to prepare powder A and powder B and mixed to prepare a mixed extract powder.

Comparative Example 3. Pear juice omitted

A mixture was obtained in the same manner as in Example 1, except that pear juice was not used.

Comparative Example 4. Aronia juice omitted

A mixture was obtained in the same manner as in Example 1, except that pear juice was not used.

Comparative Example 5. Omitting thistle

The same procedure as in Example 1 was performed, except that a mixed extract was obtained without using milk thistle when the mixed extract was prepared, and then pear juice, aronia juice, honey and stevia were mixed to obtain a mixture.

Comparative Example 6. Dandelion omitted

It was carried out in the same manner as in Example 1, except that the mixed extract was obtained without using dandelion when preparing the mixed extract, and then the pear juice, aronia juice, honey and stevia were mixed to obtain a mixture.

<Test Example>

Test Example 1. Evaluation of Efficacy of Reducing Ethanol in Blood

The effect of the mixtures prepared in Examples and Comparative Examples of the present invention on an increase in blood ethanol concentration according to intake of ethanol was confirmed.

Male 6-week-old (n=5) SD rats were divided into 8 groups, and the first group was a control group without any administration, and the second group was administered orally with 20% (v/v) ethanol for 2 days. Administration, the 3rd day is an ethanol administration group (alcohol+vehicle) administered with 40% (v/v) ethanol, and the third to eighth groups are administration groups in which the mixtures of Example 1 and Comparative Examples 1 to 5 are administered, respectively . The group administered with the mixture of Examples and Comparative Examples was pre-treated with the mixtures prepared in Examples and Comparative Examples at 400 mg/kg for 3 days before ethanol administration, and when ethanol was administered, 400 mg/kg/kg 30 minutes before ethanol administration kg of the mixtures prepared in Examples and Comparative Examples were administered, respectively. After the last administration of ethanol, the mixtures prepared in Examples and Comparative Examples, 30 minutes, 60 minutes, 120 minutes, and 180 minutes after the orbital blood sample and the blood sampled from the abdominal vena cava were centrifuged at 3000 rpm for 20 minutes to the serum obtained. The concentration of ethanol contained was measured using an ethanol measuring kit (abcam, ab65343).

division Blood Ethanol Concentration (nM) 0 minutes 30 minutes 60 minutes 120 minutes 180 minutes normal group 10.2 10.4 12.1 11.8 11.4 Ethanol administration group 10.5 14.6 17.5 18.1 14.9 Example 1 Administration group 10.1 12.7 13.9 12.5 11.8 Example 2 Administration group 10.2 12.2 13.5 12.0 11.1 Comparative Example 1 Administration group 10.2 15.0 17.7 17.6 14.2 Comparative Example 2 Administration group 10.2 14.4 17.3 17.1 14.0 Comparative Example 3 Administration group 10.2 14.1 17.0 16.9 13.9 Comparative Example 4 Administration group 10.5 14.2 16.9 16.5 13.7 Comparative Example 5 Administration group 10.1 14.0 16.8 16.7 14.1 Comparative Example 6 Administration group 10.3 13.8 16.2 16.1 13.8

As shown in Table 1 above, the Example 1 administration group and the Example 2 administration group, which ingested the mixture prepared according to Example 1 of the present invention, increased up to 60 minutes, and gradually recovered and returned to an almost normal concentration after 3 hours. confirmed that.

Moreover, it was confirmed that the Example 1 administration group and the Example 2 administration group had a lower blood ethanol concentration over all time compared to the ethanol administration group and the administration group of Comparative Examples 1 to 6, and the administration group of Comparative Examples 1 to 6 had a blood ethanol concentration up to 60 minutes. It was confirmed that the concentration of ethanol in the blood gradually decreased after 120 minutes after the concentration was increased and maintained at 60 to 120 minutes.

Although the administration group of Comparative Examples 1 to 6 had a lower blood ethanol concentration from 120 minutes, it was confirmed that it was higher than that of the normal group.

Test Example 2. Measurement of aldehyde dehydrogenase (ALDH) activity and acetaldehyde (ADH) production in blood

ALDH activity and ADH content were measured using the serum obtained in Test Example 1. ALDH activity was measured using an aldehyde dehydrogenase activity colorimetric assay kit (BioVision), and ADH was measured using an aldehyde quantification assay kit.

division ALDH activity (mU) 0 minutes 30 minutes 60 minutes 120 minutes 180 minutes normal group 1.8 1.4 1.6 1.5 1.8 Ethanol administration group 2.1 1.7 1.5 1.3 0.8 Example 1 Administration group 1.6 1.6 1.8 1.6 1.5 Example 2 Administration group 1.7 1.5 1.7 1.5 1.5 Comparative Example 1 Administration group 1.9 1.4 1.3 1.3 0.8 Comparative Example 2 Administration group 1.8 1.4 1.3 1.4 0.9 Comparative Example 3 Administration group 1.9 1.4 1.4 1.3 1.1 Comparative Example 4 Administration group 1.8 1.5 1.6 1.3 1.1 Comparative Example 5 Administration group 1.8 1.4 1.3 1.4 1.0 Comparative Example 6 Administration group 1.9 1.5 1.5 1.4 1.2

As shown in Table 2 above, the activity of aldehyde dehydrogenase showed a tendency to gradually decrease by ethanol administration, but Example 1 administration group and Example 2 ingesting the mixture prepared according to Examples 1 and 2 of the present invention In the administration group, it was confirmed that the activity of aldehyde dehydrogenase was maintained.

Moreover, it was confirmed that the activity of the aldehyde dehydrogenase was maintained in the group administered in Example 1 and the group administered in Example 2, compared to the ethanol administration group in which the activity of aldehyde dehydrogenase was gradually decreased and compared to the administration group of Comparative Examples 1 to 6.

division ADH content (mM/ml) 0 minutes 30 minutes 60 minutes 120 minutes 180 minutes normal group 97.8 82.4 99.6 102.4 91.5 Ethanol administration group 93.7 80.4 108.6 122.1 123.4 Example 1 Administration group 92.4 81.1 101.7 102.6 96.5 Example 2 Administration group 92.8 83.5 100.5 102.0 95.3 Comparative Example 1 Administration group 93.7 83.5 107.7 121.4 119.7 Comparative Example 2 Administration group 93.4 83.9 106.4 120.1 119.2 Comparative Example 3 Administration group 92.9 94.1 104.0 116.8 109.5 Comparative Example 4 Administration group 92.5 82.7 103.5 114.1 111.5 Comparative Example 5 Administration group 94.7 84.0 104.7 116.5 113.0 Comparative Example 6 Administration group 94.8 83.6 102.8 108.7 108.9

As shown in Table 3 above, the blood concentration of acetaldehyde in the ethanol-administered group continued to rise for 3 hours, but the Example 1 administration group and the Example 2 administration group ingested the mixture prepared according to Examples 1 and 2 of the present invention It was confirmed that it was maintained at a level almost similar to that of the normal group.

Moreover, it was confirmed that the Example 1 administration group and the Example 2 administration group maintained a low blood concentration of acetaldehyde at all times compared to the ethanol administration group and the administration group of Comparative Examples 1 to 6.

Test Example 3. Hangover Relief Related Sensory Test

The effect of improving hangover and fatigue was measured after ingesting the mixture prepared in Example 1 before drinking for 40 men and women aged 27 to 48, who drink frequently (half to two bottles of soju, on average, 3 times a week). .

Hangover improvement effect 20 headache relief 10 Fatigue improvement 3 easy to wake up in the morning 5 no effect 2

As shown in Table 4 above, 50% of clinical subjects recognized the effect of improving hangover, followed by improvement in headaches and a majority of opinions that it is easy to wake up in the morning.

Test Example 4. Measurement of GOT and GPT levels _ Liver disease

The blood collected from the abdominal artery of the rat in Test Example 1 was left at room temperature for 30 minutes and then centrifuged at 3,000 rpm at 4 ° C. for 15 minutes to separate the serum, and then the serum enzyme was analyzed using an automatic serum analyzer (Dri-ch em 2000, Fujifilm). , Tokyo, Japane) were used for analysis. Four types of serum enzymes were measured: glutamate-pyruvate (GPT), glutamate-oxaloacetlate (GOT), g-GT (gammaphosphatase), and alkaline phosphatase (AP). As enzymes used for the diagnosis of liver disease, GOT, GPT, g-GT, and AP tests are widely used. The increase in the activity of these enzymes is not only highly correlated with the degree of cytotoxicity, but also fluctuates more sensitively than other blood enzymes.

Among these experiments, blood GOT and GPT levels are shown in Table 5 below.

division GOT(U/L) GPT(U/L) normal group 75.9 51.2 Ethanol administration group 120.6 85.8 Example 1 Administration group 78.7 64.2 Example 2 Administration group 78.0 60.8 Comparative Example 1 Administration group 104.7 82.8 Comparative Example 2 Administration group 103.1 81.3 Comparative Example 3 Administration group 97.2 76.8 Comparative Example 4 Administration group 96.5 77.1 Comparative Example 5 Administration group 98.0 77.9 Comparative Example 6 Administration group 97.4 77.0

As shown in Table 5 above, it was confirmed that the GOT and GPT values were maintained at almost the same level as the normal group in the Example 1 administration group and the Example 2 administration group ingesting the mixture prepared according to Example 1 of the present invention. .

On the other hand, it was confirmed that the administration group of Comparative Examples 1 to 6 had lower values of GOT and GPT compared to the administration group of Example 1 and Example 2 administration group.

Test Example 5. AP Level Measurement_ Liver Disease

division AP(U/L) normal group 153 Ethanol administration group 280 Example 1 Administration group 179 Example 2 Administration group 169 Comparative Example 1 Administration group 249 Comparative Example 2 Administration group 240 Comparative Example 3 Administration group 206 Comparative Example 4 Administration group 210 Comparative Example 5 Administration group 209 Comparative Example 6 Administration group 208

As shown in Table 6 above, it was confirmed that the AP level was maintained at a level almost similar to that of the normal group in the Example 1 administration group and the Example 2 administration group ingesting the mixture prepared according to Examples 1 and 2 of the present invention. .

On the other hand, it was confirmed that the administration group of Comparative Examples 1 to 6 had lower AP values than the Example 1 administration group and the Example 2 administration group.

Test Example 6. Measurement of g-GT level _ Liver disease

division g-GT (U/L) normal group 5.8 Ethanol administration group 6.7 Example 1 Administration group 5.9 Example 2 Administration group 5.7 Comparative Example 1 Administration group 6.6 Comparative Example 2 Administration group 6.5 Comparative Example 3 Administration group 6.4 Comparative Example 4 Administration group 6.3 Comparative Example 5 Administration group 6.3 Comparative Example 6 Administration group 6.4

As shown in Table 7 above, in the Example 1 administration group and the Example 2 administration group ingesting the mixture prepared according to Examples 1 and 2 of the present invention, the g-GT level was maintained at a level almost similar to that of the normal group. Confirmed.

On the other hand, it was confirmed that the administration group of Comparative Examples 1 to 6 had a lower level of g-GT compared to the administration group of Example 1 and Example 2 administration group.

According to these results, it means that the administration group of Example 1 and the administration group of Example 2 are effective for liver disease compared to the administration group of Comparative Examples 1 to 6.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Preparation of powder

500 mg of the mixture powder obtained in Example 1

Lactose 100 mg

talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2. Preparation of tablets

300 mg of the mixture powder obtained in Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3. Preparation of capsules

200 mg of the mixture powder obtained in Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

600 mg of the mixture powder obtained in Example 1

mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

Formulation Example 5. Preparation of liquid formulation

4 g of the mixture powder obtained in Example 1

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 g by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.

Formulation Example 6. Preparation of granules

1,000 mg of the mixture powder obtained in Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

1.7 mg of nicotinic acid amide

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

potassium phosphate monobasic 15 mg

Dicalcium Phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the production of a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

1,000 mg of the mixture powder obtained in Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use.

Claims (8)

100 parts by weight of a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; 1 to 30 parts by weight of pear juice; 1 to 20 parts by weight of aronia juice; And 0.1 to 5 parts by weight of honey; contains a mixture consisting of an active ingredient,
The composition for liver protection or hangover relief, characterized in that the milk thistle is a mixture of flowers, stems and leaves, and roots of thistle in a weight ratio of 1: 14-25: 0.1-0.9. The composition for liver protection or hangover relief according to claim 1, wherein the mixed extract is an extract obtained by mixing milk thistle and dandelion in a weight ratio of 1: 0.2-1 and extracted at 80 to 100 °C. delete delete 100 parts by weight of a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; 1 to 30 parts by weight of pear juice; 1 to 20 parts by weight of aronia juice; And 0.1 to 5 parts by weight of honey; contains a mixture consisting of an active ingredient,
The milk thistle is a pharmaceutical composition for the prevention or treatment of alcoholic liver disease, characterized in that the mixture of flowers, stems and leaves, and roots of thistles in a weight ratio of 1:14-25:0.1-0.9. The pharmaceutical composition for preventing or treating alcoholic liver disease according to claim 5, wherein the alcoholic liver disease is alcoholic hepatitis, alcoholic fatty liver or alcoholic cirrhosis. 100 parts by weight of a mixed extract obtained by extracting a mixture consisting of milk thistle and dandelion; 1 to 30 parts by weight of pear juice; 1 to 20 parts by weight of aronia juice; And 0.1 to 5 parts by weight of honey; contains a mixture consisting of an active ingredient,
The milk thistle is a food composition for the prevention or improvement of alcoholic liver disease, characterized in that the mixture of flowers, stems and leaves, and roots of thistle in a weight ratio of 1: 14-25: 0.1-0.9. (A) obtaining a mixed extract by mixing and extracting milk thistle and dandelion;
(B) mixing by adding 1 to 30 parts by weight of pear juice and 1 to 20 parts by weight of aronia juice to 100 parts by weight of the mixed extract; and
(C) adding and mixing 0.1 to 5 parts by weight of honey and stevia to the mixture in which the pear juice and aronia juice are mixed;
The method for producing a composition for liver protection or hangover relief, characterized in that the milk thistle is a mixture of flowers, stems and leaves, and roots of thistle in a weight ratio of 1:14-25:0.1-0.9.