KR20220142074A - Composition for improving liver function comprising Cudrania tricuspidata fruit and white grub as an effective ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing or treating a liver disease for improving a liver function comprising a Cudrania tricuspidata fruit extract and cicada larva as active ingredients. In accordance to thereof, the present invention is harmless to the human body as a natural material, and eliminates a fishy taste and a bad smell, thereby having excellence in improving a liver function and preventing or treating a liver disease while being easily consumed.

Description

Composition for improving liver function comprising Cudrania tricuspidata fruit and white grub as an effective ingredient}

The present invention relates to a composition for improving liver function, preventing or treating liver disease, comprising an extract of cucurbita fruit and slugs as active ingredients.

The liver is the largest organ in the human body, and its main function is to process various substances introduced from outside the body or generated in the body, and to synthesize and supply important substances. In addition, there are several proteins in the blood that play an important role in our body, and about 90% of them are made in the liver. In addition, the liver plays the role of detoxification and immune organs, and various drugs and harmful substances entering our body are converted into harmless substances in the liver and excreted through urine or bile. In addition, the liver has immune cells called Kupffer cells, which can break down bacteria, toxins, or foreign substances that enter the body and discharge them out of the body.

As such, since the liver performs various and important functions, various problems occur when liver function is deteriorated. In particular, fatty liver, which may be caused by excessive alcohol intake, is one of the representative liver diseases. Most of the fat accumulated in fatty liver is triglyceride, and fatty liver can be largely divided into alcoholic fatty liver caused by excessive drinking and non-alcoholic fatty liver caused by obesity, diabetes, hyperlipidemia, or drugs. Alcoholic fatty liver occurs because excessive alcohol intake promotes fat synthesis in the liver and prevents normal energy metabolism.

Modern people cannot avoid the accumulation of toxins in the human body due to air pollution, pollution by pesticides or heavy metals, and overdose of antibiotics. In addition, since the liver function may be deteriorated along with the decrease in immunity when exposed to excessive stress, the development of foods or drugs for preventing the deterioration of the liver function and improving the liver function has been steadily made.

On the other hand, recently, functional foods using insects or pharmaceutical compositions using insects as a raw material of natural products have been developed. These insects have also been used as traditional herbal medicines, and there are about 30 species such as slugs, silkworms, cicadas, Cordyceps, and centipedes.

Among them, the slug is a beetle larva, which is a larva of insects centered on chafers and stag beetles, and the larva of Protaetia brevitarsis or the larva of Allomyrina dichotoma is representative. Although slugs are widely known as traditional herbal medicines, they are difficult to consume due to their fishy taste and odor.

Also, Cujippong is a small tall tree that grows at the foot of a sunny mountain or around a village. It is 3-8 m high. There are thorns on the branches. The leaves are ovate, sometimes divided into 3 parts, 6-10cm long, 3-6cm wide, and the top gradually becomes narrower and the tip becomes dull. Flowers bloom in male and female trees in May-June. In male inflorescences, small flowers are gathered to form a head, and short, soft hairs are gathered. The fruit is aqueous, several are gathered to form a ball, and is fleshy and ripens from red to black. It grows mainly in the western and southern coastal areas south of Hwanghae Province in Korea. It is also distributed in Japan and China. The fruits are edible or medicinal, and the leaves are used as feed for silkworms.

Korean Patent No. 10-1616688 Korean Patent No. 10-1328668

An object of the present invention is a liver function comprising, as active ingredients, a cudrania tricuspidata fruit extract and slugs, which are harmless to the human body as a natural material and are effective in relieving hangover and improving liver function while being easy to consume by removing fishy taste and odor To provide a food composition for improvement.

Another object of the present invention is to include, as an active ingredient, a Cudrania tricuspidata fruit extract and slugs, which are harmless to the human body as a natural material and have an effect of preventing or treating liver diseases such as alcoholic fatty liver while being easy to ingest by removing the fishy taste and odor. To provide a pharmaceutical composition for preventing or treating liver disease.

According to one aspect of the invention,

Cudrania tricuspidata A food composition for improving liver function is provided, comprising a fruit extract and slugs as active ingredients.

The kkujippong fruit extract may be extracted with water, alcohol having 1-4 carbon atoms, or a mixed solvent thereof.

The slug may be a dry powder of larvae of Protaetia brevitarsis .

The food composition may include 5 to 10 parts by weight of slugs based on 100 parts by weight of the cujibbong fruit extract.

The food composition may be for relieving a hangover or for improving alcoholic liver damage.

According to another aspect of the present invention,

Cudrania tricuspidata ) There is provided a pharmaceutical composition for preventing or treating liver disease comprising a fruit extract and slugs as active ingredients.

The liver disease may be any one selected from fatty liver, alcoholic fatty liver, hepatitis, cirrhosis and liver cancer.

The pharmaceutical composition may be used for reducing the blood concentration of one or more selected from glutamyl oxaloacetic transaminase (GOT), glutamyl pyruvic transaminase (GPT), and lactate dehydrogenase (LDH).

The pharmaceutical composition may be used for enhancing the expression of one or more antioxidant enzymes selected from SOD (superoxide dismutase) and catalase in liver tissue.

The food composition for improving liver function, comprising the Cudrania tricuspidata fruit extract and slugs as active ingredients, is a natural material that is harmless to the human body and removes fishy taste and odor, so it is easy to consume and is effective in relieving a hangover and improving liver function. It has an excellent effect.

The pharmaceutical composition for preventing or treating liver disease, comprising the Cudrania tricuspidata fruit extract and slugs as active ingredients, is harmless to the human body as a natural material, and it is easy to ingest by removing the fishy taste and odor, and liver disease such as alcoholic fatty liver. It has excellent preventive or therapeutic effects.

1 is a blood GOT and GPT concentration analysis result according to Experimental Example 1. FIG.
2 is a blood LDH effect analysis result according to Experimental Example 2.
3 is a measurement result of weight gain (%) according to Experimental Example 3.
4 is a measurement result of liver weight change according to Experimental Example 3.
5 is a result of analysis of the expression of antioxidant enzymes in liver tissue according to Experimental Example 4.
6 is a result of DPPH radical scavenging ability analysis according to Experimental Example 4.

Hereinafter, the present invention will be described in detail.

The pharmaceutical composition for preventing or treating liver disease of the present invention includes a Cudrania tricuspidata fruit extract and slugs as active ingredients.

The extraction solvent for extracting the kkujippong fruit extract is water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. The lower alcohol may include 20 to 80% methanol, ethanol, butanol or propanol.

의 열수에서 1 내지 10시간 동안 추출한 것이 바람직하다.The extraction solvent is not particularly limited, but 80 to 100

It is preferable to extract for 1 to 10 hours in hot water of

In the present specification, the term 'extract' used while referring to the cucurbita fruit extract includes not only the crude extract obtained by treating the extraction solvent, but also the processed product of the cujippong fruit extract. For example, the kujippong fruit extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

In addition, the cujippong fruit extract of the present invention has a meaning including a cujippong fruit processed product formulated to be administered to an animal in a broad sense, for example, cujippong fruit powder. Although the experiment was carried out with cujippong fruit in the present invention, it will be expected by those skilled in the art that the desired effect can be achieved even in the same form as the cujippong fruit processed product.

The slug may be a dry powder of larvae of Protaetia brevitarsis.

의 열풍에 건조시킨 후 분말화한 것일 수 있으나 분말화하는 방법은 특별히 한정되지 않는다.The dry powder is 60 to 80 slugs

It may be powdered after drying in the hot air of a, but the method of powdering is not particularly limited.

The pharmaceutical composition preferably contains 5 to 10 parts by weight of slugs with respect to 100 parts by weight of the kujippong fruit extract. If the slug content is less than 5 parts by weight, liver disease prevention or treatment efficacy may be reduced, and if it exceeds 10 parts by weight, fishy taste and odor may increase, and liver disease prevention or treatment efficacy may be reduced. .

The liver disease may be fatty liver, alcoholic fatty liver, hepatitis, cirrhosis, and the like.

In addition, the pharmaceutical composition has a characteristic of reducing the blood concentration of one or more selected from glutamyl oxaloacetic transaminase (GOT), glutamyl pyruvic transaminase (GPT), and lactate dehydrogenase (LDH).

In addition, the pharmaceutical composition can prevent or treat liver disease by enhancing the expression of one or more antioxidant enzymes selected from among superoxide dismutase (SOD) and catalase in liver tissue.

On the other hand, in the present specification, the term 'comprising as an active ingredient' means including an amount sufficient to achieve the efficacy or activity of the complex including the cucurbita fruit extract and slugs. In one embodiment of the present invention, the complex comprising cucurbita fruit extract and slugs in the composition of the present invention is, for example, 0.001 mg/kg or more, preferably 0.1 mg/kg or more, more preferably 10 mg/kg or more. kg or more, more preferably 100 mg/kg. Since the complex containing the cucurbita fruit extract and slugs is a natural product and there is no side effect to the human body even when administered in excess, the upper quantitative limit of the complex including the cucurbita fruit extract and slugs contained in the composition of the present invention is selected and carried out by those skilled in the art by selecting within an appropriate range. can do.

The pharmaceutical composition of the present invention may be prepared by using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, a disintegrant, a sweetener, a binder, a coating agent, a swelling agent, a lubricant, and a lubricant. agent or flavoring agent, etc. may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For formulation in the form of, for example, tablets or capsules, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration mode, age, weight, sex, morbidity, food, administration time, administration route, excretion rate, and response sensitivity of the patient, usually Thus, a skilled physician can easily determine and prescribe an effective dosage for the desired treatment or prevention. According to a preferred embodiment of the present invention, the daily dose of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention may be prepared in a unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient, or may be prepared by internalizing in a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion in oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or stabilizer.

In addition, the present invention provides a food composition for improving liver function comprising an extract of Cudrania tricuspidata fruit and slugs as active ingredients.

In the food composition for improving liver function of the present invention, specific descriptions of the cujibbong fruit extract, slugs, composition ratio, use, and function are the same as those of the pharmaceutical composition, so the specific details will be referred to the above description.

The food composition is preferably used for relieving a hangover or for improving alcoholic liver damage.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, sweets, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice cream, vitamin complexes, health supplements etc.

The food composition of the present invention may include not only a complex including a cucurbita fruit extract and slugs as active ingredients, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, and seasonings. and flavoring agents. Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink and beverage, citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts, red ginseng, in addition to the complex containing the cucurbita fruit extract and slugs of the present invention and the like may be further included.

The present invention provides a health functional food comprising a food composition for improving liver function comprising an extract of Cudrania tricuspidata fruit and slugs as active ingredients. Health functional food is a food prepared by adding a complex containing cucurbita fruit extract and slugs to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspension, etc. It means to bring a specific effect, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The amount of addition of the complex containing cucurbita fruit extract and slugs in such a health functional food cannot be uniformly defined as it varies depending on the type of health functional food subject to it, but it can be added in a range that does not impair the original taste of the food. , It is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight, based on the target food. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides a use of Cudrania tricuspidata fruit extract and slugs for the manufacture of a medicament or food for improving liver function, preventing or treating liver disease.

In addition, the present invention provides a method for improving liver function, preventing or treating liver disease, comprising administering an effective amount of Cudrania tricuspidata fruit extract and slugs to a mammal.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term "effective amount" refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as conceived by a researcher, veterinarian, physician or other clinician, which is the disease in question. or an amount that induces alleviation of the symptoms of the disorder. The effective amount and frequency of administration for the active ingredient of the present invention may vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of active ingredients and other components contained in the composition, the type of formulation, and the age, weight, and general health of the patient It can be adjusted according to various factors including condition, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and concurrently used drugs. In the prevention, treatment or improvement method of the present invention, in the case of adults, when administering the complex containing the kujipon fruit extract and slugs once to several times a day, it is recommended to administer at a dose of 0.001 g/kg to 10 g/kg. desirable.

In the treatment method of the present invention, the composition comprising Cudrania tricuspidata fruit extract and slugs as active ingredients is administered by oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, or intradermal routes. It can be administered in a conventional manner.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

[Example]

Example 1: Cujippong fruit hot water extract and slugs complex

After pulverizing the Cujippong fruit, it was extracted by treatment with hot water at 90° C. for 5 hours, filtered, and the supernatant was dried by spray-drying to obtain a hot water extract powder from Cujippong fruit.

In addition, the larvae of Protaetia brevitarsis were dried by hot air at 70° C. to obtain a slug powder, and the hot water extract of Cudrania fruit and the powder of slugs were mixed in a weight ratio of 95:5 to prepare a hot water extract of cucurbita fruit and a slug complex. did.

Comparative Example 1: slug powder

The slug powder obtained under the same conditions as in Example 1 was used alone.

Comparative Example 2: Cujippong fruit hot water extract

The hot water extract powder of Cujippong fruit obtained under the same conditions as in Example 1 was used alone.

[Experimental example]

Experimental Example 1: Analysis of GOT and GPT concentration in blood

The inhibitory effect on alcoholic hepatotoxicity by oral administration of the cujippong fruit extract + slug squirrel complex of Example 1, the slug powder of Comparative Example 1, and the cujibbong fruit extract of Comparative Example 2 was analyzed through GOT and GPT blood concentrations. Specifically, hepatotoxicity was induced by oral administration of 25% ethanol (EtOH) to 5-week-old male Balb/c mice at an amount of 5 g/kg once a day for a total of 7 days. In addition, the samples of Example 1, Comparative Example 1, and Comparative Example 2 were orally administered at an amount of 5 mg/mouse from 2 days before ethanol administration for 9 days. Blood GOT and GPT concentrations were quantified using DriChem 3500i after separating a serum sample from blood on the first day after the end of ethanol administration, and the results are shown in FIG. 1 . (a) is the blood GOT concentration, (b) is the blood GPT concentration.

According to this, it was found that the blood GOT and GPT concentrations were significantly decreased (**p<0.01) in the group administered with the Kujippong fruit extract + slugs complex of Example 1 (**p<0.01), whereas the slugs alone administration group of Comparative Example 1 , There was no significant difference in the group administered with the cujippong fruit extract of Comparative Example 2. Statistical significance was examined through Student's two tailed t-test for the group treated with ethanol only. In other words, it was confirmed that the cujippong fruit extract + slugs complex of Example 1 of the present invention was more effective in inhibiting alcoholic hepatotoxicity than the cujibbong fruit extract alone or slugs alone.

Experimental Example 2: Analysis of blood LDH effect

It is known that the blood concentration of LDH enzyme in hepatocytes increases due to hepatocyte damage during the onset of liver disease. The inhibitory effect of the cujippong fruit extract+slug complex of Example 1, the slug powder of Comparative Example 1, and the cucurbita fruit extract sample of Comparative Example 2 on the increase in blood LDH concentration in liver disease induced by alcohol administration was analyzed. Specifically, hepatotoxicity was induced by oral administration of 25% ethanol (EtOH) to 5-week-old male Balb/c mice at an amount of 5 g/kg once a day for a total of 7 days. In addition, the samples of Example 1, Comparative Example 1, and Comparative Example 2 were orally administered at an amount of 5 mg/mouse from 2 days before ethanol administration for 9 days. The concentration of LDH in the blood was quantified using DriChem 3500i by separating a serum sample from the blood on the first day after the end of ethanol administration, and the results are shown in FIG. 2 .

According to this, it was found that the concentration of LDH in the blood was significantly decreased (*p<0.05) in the group administered with the Kujippong fruit extract + slugs complex of Example 1 (*p<0.05), compared with the slugs alone administration group of Comparative Example 1, compared There was no significant difference in the group administered with the cucurbita fruit extract of Example 2. Statistical significance was examined through Student's two tailed t-test for the group treated with ethanol only. In other words, it was found that the cujibbong fruit extract + slugs complex of Example 1 of the present invention was superior to the effect of inhibiting the rise in blood LDH concentration compared to the cujibbong fruit extract alone or the slugs alone.

Experimental Example 3: Analysis of the effect of inhibiting alcohol-induced weight loss and liver weight loss

The inhibitory effect of the cujippong fruit extract+slug complex of Example 1, the slug powder of Comparative Example 1, and the cujibbong fruit extract sample of Comparative Example 2 on weight and liver weight reduction induced by alcohol administration was analyzed. Specifically, 25% ethanol (EtOH) was orally administered to 5-week-old male Balb/c mice at an amount of 5 g/kg once a day for a total of 7 days to induce a decrease in body weight and liver weight. In addition, the samples of Example 1, Comparative Example 1, and Comparative Example 2 were orally administered at an amount of 5 mg/mouse from 2 days before ethanol administration for 9 days. Body weight and liver weight were measured 1 after the end of ethanol administration, and the measurement results for the weight gain (%) are shown in FIG. 3 , and the measurement results of the change in liver weight are shown in FIG. 4 .

According to this, in Example 1 kkujippong fruit extract + slughead complex administration group showed a significant increase in body weight compared to the control group, and the degree of recovery of liver weight was slightly increased, but there was no significant difference.

Experimental Example 4: Antioxidant enzyme expression analysis in liver tissue

The expression synergistic effect of SOD (superoxide dismutase) and catalase, which are oxidative reductases in liver tissue, of Cuji mulberry fruit extract + Slugfish complex of Example 1, Slugfish powder of Comparative Example 1, and Cuji mulberry fruit extract samples of Comparative Example 2 analyzed. Specifically, hepatotoxicity was induced by oral administration of 25% ethanol (EtOH) to 5-week-old male Balb/c mice at an amount of 5 g/kg once a day for a total of 7 days. In addition, the samples of Example 1, Comparative Example 1, and Comparative Example 2 were orally administered at an amount of 5 mg/mouse from 2 days before ethanol administration for 9 days. The concentration of LDH in the blood was quantified using a kit for quantifying the expression level of each enzyme after extracting the liver tissue to measure the expression level of SOD and catalase, which are oxidation reductase enzymes, on the 1st day after the end of ethanol administration, and SOD in the liver tissue The concentration measurement result (a) and the catalase concentration measurement result (b) are shown in FIG. 5 .

According to this, the concentration of SOD and catalase in the liver tissue was significantly increased in the group administered with the Kujippong fruit extract + slughead complex of Example 1 compared to the control group. That is, it can be seen that the kkujippong fruit extract + slugwort complex of the present invention has an effect of protecting the liver tissue by promoting the expression of antioxidant enzymes in the liver tissue.

On the other hand, DPPH (1,1'-dipheny-2-picrylhydrazyl) radical scavenging ability was analyzed for the antioxidant effect according to the dosage of the cucurbita fruit extract + slug larvae complex of Example 1. Specifically, the DPPH radical scavenging ability was determined by adding 20 μl of the composite sample of Example 1 to 80 μl of 0.2 mM DPPH ethanol solution by concentration, mixing for 10 seconds, reacting at room temperature for 10 minutes, and measuring absorbance at 492 nm using an ELISA reader. analysis, and the results are shown in FIG. 6 .

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Preparation of powder

30 mg of slug powder of Example 1

500 mg of Kujippong fruit extract powder of Example 1

Lactose 100 mg

talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2. Preparation of tablets

20 mg of slug powder of Example 1

300 mg of Kujippong fruit extract powder of Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3. Preparation of capsules

12 mg of slug powder of Example 1

200 mg of Cujipon fruit extract of Preparation Example 2

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

32 mg of slug powder of Example 1

600 mg of Cujipon fruit extract of Preparation Example 2

mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

Formulation Example 5. Preparation of liquid formulation

400 mg of slug powder of Example 1

7.5 g of Cujipon fruit extract of Preparation Example 2

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added and dissolved in purified water, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 g by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.

Formulation Example 6. Preparation of granules

100 mg of slug powder of Example 1

1,900 mg of Cujippong fruit extract of Preparation Example 2

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

100 mg of slug powder of Example 1

1,900 mg of Cujippong fruit extract of Preparation Example 2

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as demand class, demanding country, and use.

In the above, although embodiments of the present invention have been described, those of ordinary skill in the art can add, change, delete or add components within the scope that does not depart from the spirit of the present invention described in the claims. The present invention may be variously modified and changed by, etc., and this will also be included within the scope of the present invention.

Claims (9)

Cudrania tricuspidata ) A food composition for improving liver function comprising a fruit extract and slugs as active ingredients. The method of claim 1,
The kkujippong fruit extract is a food composition for improving liver function, characterized in that it is extracted by water, an alcohol having 1-4 carbon atoms, or a mixed solvent thereof. The method of claim 1,
The slug is a food composition for improving liver function, characterized in that it is a dry powder of larvae of Protaetia brevitarsis . The method of claim 1,
The food composition is a food composition for improving liver function, characterized in that it contains 5 to 10 parts by weight of slugs with respect to 100 parts by weight of the cucurbita fruit extract. The method of claim 1,
The food composition is a food composition for improving liver function, characterized in that it is for relieving a hangover or for improving alcoholic liver damage. Cudrania tricuspidata ) A pharmaceutical composition for preventing or treating liver disease comprising a fruit extract and slugs as active ingredients. 7. The method of claim 6,
The liver disease is a liver disease prevention or treatment pharmaceutical composition, characterized in that any one selected from fatty liver, alcoholic fatty liver, hepatitis, liver cirrhosis and liver cancer. 7. The method of claim 6,
The pharmaceutical composition is a pharmaceutical composition for preventing or treating liver disease, characterized in that it is used for reducing the blood concentration of one or more selected from glutamyl oxaloacetic transaminase (GOT), glutamyl pyruvic transaminase (GPT) and lactate dehydrogenase (LDH). 7. The method of claim 6,
The pharmaceutical composition is a pharmaceutical composition for preventing or treating liver disease, characterized in that it is used for enhancing the expression of one or more antioxidant enzymes selected from SOD (superoxide dismutase) and catalase in liver tissue.

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