KR102500034B1 - A composition comprising an extract of Thymus quinquecostatus Celak for treating and preventing hangover - Google Patents

Abstract

The present invention relates to a composition and health functional food for improving liver function and preventing and treating hangovers containing thyme extract as an active ingredient. In vitro experiments such as alcohol dehydrogenase (ADH) activity measurement experiment and aldehyde dehydrogenase (ALDH) activity measurement experiment (Experimental Example 1) for the thyme extract of the present invention; Prevention of hangover by confirming that the samples of the present invention exhibit strong liver function improvement and hangover treatment and prevention effects through animal experiments (Experimental Example 2), etc. And it can be usefully used in pharmaceutical compositions for treatment, health functional food and health supplement food.

Description

Composition for preventing or treating hangover containing thyme extract {A composition comprising an extract of Thymus quinquecostatus Celak for treating and preventing hangover}

The present invention relates to a composition for preventing or treating hangover containing a thyme extract.

[Document 1] Swift R et al., Alcohol Health Res World 1998;22:54-60

[Document 2] Korea Patent Registration No. 10-0620093

[Document 3] Korean Patent Registration No. 10-1723022

[Document 4] Kwon et al., Journal of the Korean Society of Applied Pharmacy, 2005, 13, p. 107

[Document 5] Korean Patent Publication No. 10-2012-0044807

[Document 6] Republic of Korea Patent Registration No. 10-0828708

[Document 7] Republic of Korea Patent Registration No. 10-0620093

[Document 8] Republic of Korea Patent Registration No. 10-1334887

[Document 9] Korean Patent Registration No. 10-1723022

[Document 10] Jeong Bo-subdeung, Dohae Hyangyakdaejeonjeon, Younglimsa, 1990, p868-869

[Document 11] Blandino, A et al., Biotechnology letters, 1997;19:651-654

[Document 12] Bostian, K et al., Biochemical Journal, 1978;173:787-798

[Document 13] You, Y et al., J Korean Soc Food Sci Nutr , 2016;10:1508-1512

The present invention relates to a composition for preventing or treating hangover, and more particularly, to a composition for preventing or treating hangover containing a thyme extract.

Alcohol acts on the central nervous system of the brain to temporarily improve mood and forget suffering, and is used as a means of socializing and food for relieving stress. However, hangover after drinking is a physical and mental phenomenon, and its typical symptoms include nausea, vomiting, dizziness, thirst, lethargy, headache, and muscle pain (Swift R et al., Alcohol Health Res World 1998;22:54- 60).

In the normal process of ethyl alcohol metabolism, ethyl alcohol introduced into the body is absorbed from the stomach or small intestine, enters the bloodstream, and is transferred to the liver. There is alcohol dehydrogenase (ADH) in hepatocytes, which oxidizes alcohol to acetaldehyde, and the acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase (ALDH) in hepatocytes, resulting in muscle or adipose tissue of the whole body and is finally decomposed into carbon dioxide gas and water. In addition, the acetaldehyde dehydrogenase includes type II, which initiates oxidation even at low concentrations of acetaldehyde, and type I, which does not work unless acetaldehyde is high in concentration, but Asians generally lack or lack type II acetaldehyde enzymes. Because of this, the oxidation of acetaldehyde is slow compared to Westerners. Unoxidized acetaldehyde and/or ethanol interfere with normal metabolism, causing various hangover symptoms. (Korean Patent Registration No. 10-0620093)

After drinking, alcohol is metabolized through three pathways. When the concentration of ethanol is low, alcohol dehydrogenase and acetaldehyde dehydrogenase present in the gastrointestinal tract or liver act. It is metabolized into acetaldehyde and acetic acid by the microsomal ethanol oxidizing system (MEOS) present in the endoplasmic reticulum, and then converted to carbon dioxide (CO ₂ ) through the action of catalase present in the peroxisome. ) and finally decomposed into water (H ₂ O). When an appropriate amount of alcohol is introduced, the above-described metabolic system works smoothly and all symptoms caused by alcohol do not occur. Persimmon, loss of concentration, heartburn and indigestion, etc. may be caused, and liver dysfunction may occur in the long term. (Korean Patent Registration No. 10-1723022).

Acetaldehyde, which is essentially generated in the metabolic process of ingested alcohol, causes the biggest cause of acute alcohol hangovers, namely nausea, vomiting, dizziness, thirst, lethargy, and muscle pain, and causes social and economic losses due to reduced work ability of the general public. do.

Hangover refers to symptoms such as headache, diarrhea, anorexia, nausea, vomiting, chills, and cold sweat that appear after drinking alcohol. It is known that the cause of a hangover is dehydration, toxicity of alcohol and alcohol metabolites (acetaldehyde, formaldehyde, acetone, etc.), and nutrient deficiency (blood sugar, vitamin, and mineral deficiency) due to malabsorption. The degree of hangover varies greatly depending on individual variation according to heredity and environmental conditions (nutritional status, exercise status, dehydration level, health status) (Kwon et al., Journal of the Korean Society of Applied Pharmacy, 2005, 13, p. 107)

In the past, a composition for relieving hangover using various extracts has been developed. For example, Korean Patent Publication No. 10-2012-0044807 discloses a composition for improving liver function or relieving hangover, containing chlorella as an active ingredient; Korean Registered Patent No. 10-0828708 discloses a composition for preventing or treating hangovers, including glutathione-containing yeast extract, emulsified zinc, Rosa roxburghi extract, Engelgarditia chrysolpsis HANCE extract, and Nelumbo nucifera extract; Republic of Korea Patent Registration No. 10-0620093 is anti-hangover containing an extract selected from the group consisting of Rosa roxburghii fruit extract, Engelharditia chrysolepis HANCE leaf extract, Nelumbo nucifera extract, or a combination thereof as an active ingredient or a therapeutic composition; Republic of Korea Patent Registration No. 10-1334887 discloses a pharmaceutical composition for preventing or relieving hangover from alcohol containing at least one selected from the group consisting of an extract of Sophora flavescens, a dried product of the extract, and a concentrate of the extract as an active ingredient. ; Korean Patent Registration No. 10-1723022 discloses a composition for preventing or relieving a hangover containing an extract of Gaepaul tree.

There has been a demand for continuous development of a hangover relieving composition that exhibits a higher sensory relieving effect than conventional hangover relieving compositions such as headache or heaviness, gastrointestinal discomfort, thirst, sweating, lethargy, and the like.

However, nowhere in the above document is there any disclosure or teaching about the therapeutic effect of the single component extract of thyme extract on hangover.

Thyme ( Thymus quinquecostatus Celak.; Lamiaceae: Labiatae) is distributed in high mountains throughout Korea, and its components include Scutellarein-heterosode, luteolin-7-glucoside Ingredients such as , carvacrol, and ursolic acid are known, and are known to be effective for bronchitis and neuritis (Jeong Bo-seop, Dohae Hyangyak Dictionary, Younglimsa, 1990, p868-869).

Therefore, as part of the research to develop an effective hangover cure, the present inventors conducted an alcohol dehydrogenase (ADH) activity measurement experiment and an aldehyde dehydrogenase (ALDH) activity measurement experiment targeting thyme extract. In vitro experiment (Experimental Example 1); Evaluation of blood acetaldehyde concentration efficacy using an acute alcohol animal model Through animal experiments (Experimental Example 2), etc., it was confirmed that the samples of the present invention exhibit strong liver function improvement and hangover treatment and prevention effects, and the present invention completed.

An object of the present invention is to provide a pharmaceutical composition for improving liver function or relieving hangover, health functional food and health supplement containing thyme extract as an active ingredient.

In order to solve the above object, the present invention provides a pharmaceutical composition for improving liver function or relieving hangover containing thyme extract as an active ingredient.

In addition, the present invention provides a health functional food for improving liver function or relieving hangover containing thyme extract as an active ingredient.

The thyme extract defined herein is a solvent selected from water including purified water, lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, or mixed solvents thereof, preferably water or a mixed solvent of water and ethanol, more preferably Contains extracts soluble in water or 10-80% ethanol.

Thyme, as defined herein, includes parts such as shoots, roots, leaves, branches, fruits, and the like.

Hereinafter, the present invention will be described in more detail.

The extract of the present invention can be obtained by the following manufacturing method.

For example, the present invention will be described in detail below.

The thyme extract of the present invention can be prepared as follows. After washing and slicing the dried thyme, water including purified water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, butanol, or a solvent selected from mixed solvents thereof, preferably water or a mixed solvent of water and ethanol, more preferably After mixing with water or 10-80% ethanol several times, ultrasonic extraction method or hot water extraction method at a temperature of 30 ℃ to 150 ℃, preferably 70 ℃ to 120 ℃ for 30 minutes to 48 hours, preferably 1 hour to 12 hours The thyme extract of the present invention can be obtained by filtering, concentrating under reduced pressure, and drying the extract obtained by repeating the room temperature extraction method or the reflux extraction method, preferably the hot water extraction method, about 1 to 20 times, preferably 2 to 10 times.

In vitro experiments such as alcohol dehydrogenase (ADH) activity measurement experiment and aldehyde dehydrogenase (ALDH) activity measurement experiment for the thyme extract obtained above (Experimental Example 1); Efficacy evaluation of blood acetaldehyde concentration using an acute alcohol animal model Improvement of liver function by confirming that the samples of the present invention exhibit strong liver function improvement and hangover treatment and prevention effects through animal experiments (Experimental Example 2) And it is possible to provide a pharmaceutical composition, health functional food and health supplement food for the prevention and treatment of hangover.

Accordingly, the present invention provides a pharmaceutical composition, health functional food or health supplement food for improving liver function and preventing and treating hangover, containing the thyme extract obtained by the above preparation method as an active ingredient.

In addition, thyme is a medicinal material that has been used as edible or herbal medicine for a long time, and has no problems such as toxicity and side effects.

The pharmaceutical composition of the present invention includes 0.1 to 50% by weight of the extract based on the total weight of the composition.

The pharmaceutical composition containing the extract of the present invention may further include suitable carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

Carriers, excipients and diluents that may be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate. , cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical dosage form of the extract of the present invention may be used in the form of a pharmaceutically acceptable salt thereof, and may also be used alone or in combination with other pharmacologically active compounds as well as in a suitable set.

Compositions containing the extract of the present invention are formulated in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterile injection solutions, respectively, according to conventional methods. and can be used.

Specifically, when formulated, it may be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules and capsules, and the like, and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, etc. ), lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solutions for oral use, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Vegetable oils such as propylene glycol, polyethylene glycol, and olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, and glycerogelatin may be used.

The preferred dosage of the extract of the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the type of drug, the route and duration of administration, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention may be administered in an amount of 0.0001 to 100 mg/kg, preferably in an amount of 0.00 1 to 100 mg/kg once or several times a day. In the composition, the extract of the present invention may be formulated in an amount of 0.0001 to 50% by weight based on the total weight of the composition.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes. All modes of administration can be envisaged, eg oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine intrathecal and intracerebroventricular injections.

In addition, the present invention provides a health functional food for improving liver function or preventing or improving hangover containing thyme extract as an active ingredient.

"Health functional food" as defined herein refers to food manufactured and processed using raw materials or ingredients having useful functionalities for the human body in accordance with the Health Functional Food Act No. 6727, and "functional" means It refers to intake for the purpose of obtaining useful effects for health purposes, such as regulating nutrients with respect to structure and function or physiological action.

The health functional food of the present invention contains the extract in a weight percentage of 0.01 to 95%, preferably 1 to 80%, based on the total weight of the composition.

In addition, for the purpose of preventing or improving diseases, pharmaceutical dosage forms such as powders, granules, tablets, capsules, pills, suspensions, emulsions, syrups, etc., or health functional foods in the form of tea bags, leachated tea, health drinks, etc. are manufactured and processing is possible.

In addition, the present invention provides a health supplement for improving liver function or relieving hangover containing thyme extract as an active ingredient.

The extract of the present invention can be used in various ways such as pharmaceuticals, foods and beverages for the prevention and treatment of target diseases. Foods to which the extract of the present invention can be added include, for example, various foods, beverages, gum, tea, vitamin complexes and health supplements, and can be used in the form of powders, granules, tablets and capsules or beverages. there is.

In addition, the health functional food may additionally contain food additives, and the suitability as a "food additive" is determined according to the general rules of the Food Additive Code and general test methods approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the relevant standards and standards.

In addition, the present invention provides a food or food additive for preventing or improving hangover containing thyme extract as an active ingredient.

Examples of items listed in the “Food Additives Codex” include chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as dark pigment, licorice extract, crystalline cellulose, and guar gum, L- Mixed preparations such as monosodium glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations may be mentioned.

Functional foods containing the extract of the present invention include bread, rice cakes, dried confections, candies, chocolates, chewing gum, confectionery products such as jams, ice creams, ice creams, ice cream products such as ice cream powders, milks, low-fat milks, and lactose-decomposed milks. , Processed milk, goat milk, fermented milk, buttermilk, concentrated milk, milk cream, buttermilk, natural cheese, processed cheese, milk powder, dairy products such as whey Processed meat products, processed egg products, meat products such as hamburgers Fish cake, ham Fish meat products such as fish meat products such as sausages and bacon Ramen, dried noodles, fresh noodles, fried noodles, deluxe dried noodles, improved deep-fried noodles, frozen noodles, pasta, etc. Fruit drinks, vegetable drinks, carbonated drinks, soy milk , beverages such as lactobacillus beverages such as yogurt, beverages such as mixed beverages, soy sauce, soybean paste, gochujang, chunjang, cheonggukjang, mixed soy sauce, vinegar, sauces, tomato ketchup, curry, seasoning foods such as dressings, margarine, shortening, and pizza. It is not limited.

The extract of the present invention may be added to food or beverage for the purpose of preventing or treating a target disease. At this time, the amount of the extract in the food or beverage is generally 0.01 to 15% by weight of the total food weight of the health food composition of the present invention, and the health drink composition is 0.02 to 30 g based on 100 ml, preferably may be added at a rate of 0.3 to 10 g.

The health beverage composition of the present invention has no particular limitations on liquid components other than containing the extract as an essential component in the indicated ratio, and may contain various flavors or natural carbohydrates as additional components like conventional beverages. Examples of the aforementioned natural carbohydrates include monosaccharides, such as disaccharides such as glucose and fructose, such as polysaccharides such as maltose and sucrose, such as common sugars such as dextrins and cyclodextrins. , sugar alcohols such as xylitol, sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g. rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can advantageously be used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, the extract of the present invention may contain fruit flesh for the production of natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not critical, but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

In vitro experiments such as alcohol dehydrogenase (ADH) activity measurement experiment and aldehyde dehydrogenase (ALDH) activity measurement experiment (Experimental Example 1) for the thyme extract of the present invention; Prevention of hangover by confirming that the samples of the present invention exhibit strong liver function improvement and hangover treatment and prevention effects through animal experiments (Experimental Example 2), etc. And it can be usefully used in pharmaceutical compositions for treatment, health functional food and health supplement food.

1 is a result of an efficacy evaluation test of acetaldehyde concentration in blood using an acute alcohol animal model of thyme.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only to illustrate the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example 1. Preparation of thyme extract

Add 400 ml of primary distilled water to 15 g of dried thyme ( Thymus quinquecostatus Celak) whole plant purchased from the Seoul Yaknyeongsi Association, extract hot water at 105 ° C for 3 hours, cool it appropriately, connect a vacuum pump, and filter paper (1 ㎛, 150 mm filter paper). After storing the filtered extract in a -80℃ cryogenic freezer (908, Forma 900 series. Thermo scientific, USA), 1.7 g of thyme extract in powder form was prepared through a freeze dryer (FD8512, Ilshin, Seoul, Korea) at -20℃. It was stored and used in the following experimental example (hereinafter referred to as “TQ”).

Experimental example 1. In vitro active experiment

Experiments were performed as follows to determine the effect of the samples of the above examples on the elimination and improvement of liver function.

1-1. Measurement of alcohol dehydrogenase (ADH) activity

In order to examine the effect of the samples of the above examples on the activity of alcohol dehydrogenase (ADH), the following experiment was performed using the method described in the literature (Blandino, A et al., Biotechnology letters, 1997;19 :651-654)

1-1-1. ready to experiment.

The lyophilized enzyme source S9 rat liver homogenate (MOLTOX ^TM , cat. NO;11-01L.2) was dissolved in 8 ml of a 0.1% bovine serum albumin (Sigma aldrich, cat NO; A9647) solution, and a 0.45 μm syringe was used. It was used after filtering with a filter.

The composition of the reaction solution was distilled water 1.4 ml, 1.0 M tris-HCl buffer pH 8.8 (iNtRON, cat NO;IBS-BT080) 0.75 ml, 20 mM β-Nicotinamide adenine dinucleotide hydrate (NAD ⁺ ,Sigma aldrich, cat NO;N7004) 0.3 ml, 0.3 ml of 100% ethanol, and 0.1 ml of Example extracts (sample concentrations of 50, 200, 400, 800, and 1200 μg/ml) are mixed.

1-1-2. Experiment process

After mixing the reaction solution except for the enzyme source in the composition ratio, 0.15 ml of the enzyme source was mixed. After the mixture was placed in a 96-well plate and reacted at 30° C. for 5 minutes, absorbance was measured at 340 nm with a device (EPOCH microplate reader, BioTek, USA).

Finally, in the activity calculation method, the group to which the extract was not added was used as a control group, and the activity of the extract was measured as a relative activity (%) for the control group.

1-1-3. Experiment results

As a result of the above experiment, as a result of the alcohol dehydrogenase (ADH) activity measurement experiment of thyme, 100.43 ± 1.19 % at 50 µg/ml concentration, 102.91 ± 0.49 % at 200 µg/ml concentration, and 108.40 ± 108.40 ± at 400 µg/ml concentration. It was confirmed that the activity increased in a concentration dependent manner, from 117.28±1.83% at 1.24% and 800 μg/ml concentrations to 128.06±1.36% at 1200 μg/ml concentrations. In addition, a significant difference was observed depending on the concentration of thyme, and at 1200 μg / ml of thyme, ADH activity significantly increased 1.28 times compared to the control group (see Table 1).

Thyme alcohol dehydrogenase (ADH) activity measurement test results Relative activity of ADH Experimental group/control group ratio control 100.00±0.49 ^e % experimental group 50 µg/ml 100.43±1.19 ^e % 1.00 200 µg/ml 102.91±0.49d ^% 1.03 400 μg/ml 108.40±1.24 ^c,** % 1.08 800 μg/ml 117.28±1.83 ^b,** % 1.17 1200 μg/ml 128.06±1.36 ^a,** % 1.28 Note: Data are presented in mean ± standard deviation. ^* p < 0.05 versus control, ^** p < 0.001 versus control

1-2. Aldehyde dehydrogenase (ALDH) activity measurement

In order to examine the effect of the samples of the above examples on the activity of aldehyde dehydrogenase (ALDH), an experiment was performed as follows using the method described in the literature (Bostian, K et al., Biochemical Journal, 1978; 173 :787-798)

1-2-1. ready to experiment.

The lyophilized enzyme source S9 rat liver homogenate (MOLTOX ^TM , cat. NO;11-01L.2) was dissolved in 8 ml of a 0.1% bovine serum albumin (Sigma aldrich, cat NO; A9647) solution, and a 0.45 μm syringe was used. It was used after filtering with a filter.

The composition of this reaction solution was distilled water 2.1 ml, 1.0 M tris-HCl buffer pH 8.0 (iNtRON, cat NO; IBS-BT019) 0.3 ml, 20 mM β-Nicotinamide adenine dinucleotide hydrate (NAD ⁺ ,Sigma aldrich, cat. NO; N7004 ) 0.1ml, 1M acetaldehyde (Sigma aldrich, cat. NO; 402788) 0.1ml, 3M KCl (potassium chloride, Sigma aldrich, cat. NO; P9541) 0.1ml, 0.33M2-mercaptoethanol (Sigma aldrich, cat. NO; M3148 ) 0.1 ml, Example sample extract 0.1 ml (sample concentrations were 50, 200, 400, 800, 1200 ㎍ / ml) were mixed.

1-2-2. Experiment method

After mixing the reaction solution except for the enzyme source in the composition ratio, 0.1 ml of the enzyme source was mixed. After the mixture was placed in a 96 well plate and reacted at 30° C. for 5 minutes, absorbance was measured at 340 nm with a device (EPOCH microplate reader, BioTek, USA).

Finally, in the activity calculation method, the group to which the extract was not added was used as a control group, and the activity of the extract was measured as a relative activity (%) for the control group.

1-2-3. Experiment result

As a result of the above experiment, the aldehyde dehydrogenase (ALDH) activity of thyme was measured, 99.63 ± 7.54% at 50 μg / ml concentration, 100.49 ± 6.30% at 200 μg / ml concentration, 105.94 ± 6.62 at 400 μg / ml concentration The activity increased in a concentration dependent manner, with %, 116.37±7.91% at 800 μg/ml concentration and 128.54±7.78% at 1200 μg/ml concentration. In addition, a significant difference was observed depending on the concentration of thyme, and at 1200 μg / ml of thyme, the ALDH activity was significantly increased by 1.29 times compared to the control group (see Table 2).

Thyme aldehyde dehydrogenase (ALDH) activity measurement test results Relative activity of ALDH Experimental group/control group ratio control group 100.00±1.59 ^c % experimental group 50 µg/ml 99.63±7.54 ^c % 1.00 200 µg/ml 100.49±6.30 ^c % 1.00 400 μg/ml 105.94±6.62 ^c % 1.06 800 μg/ml 116.37±7.91 ^b,* % 1.16 1200 μg/ml 128.54±7.78 ^a,** % 1.29 Note: Data are presented in mean ± standard deviation. ^* p < 0.05 versus control, ^** p < 0.001 versus control

Experimental example 2. In vivo Efficacy evaluation

In order to confirm the hangover relief and liver function improvement effects of the samples of the above examples, an experiment to evaluate the effect of acetaldehyde concentration in blood using an acute alcohol animal model was performed by applying the method described in the existing literature (You, Y et al ., J Korean Soc Food Sci Nutr , 2016;10:1508-1512).

2-1. Experiment preparation

This study was conducted according to the ethical standards for experimental animals (Institutional Animal Care and Use Committee, JSR-2020-02-004-001-A).

Experimental animals were 8-week-old 250-300 g Sprague-Dawley rats (male) provided by DBL (Eumseong-gun, Korea), and after a week-long adaptation period, they were raised in pairs in plastic cages.

The breeding farm was controlled for 12 hours from 7:00 am to 7:00 pm by artificial lighting, the indoor temperature was maintained at 18-23 ° C and the humidity was 40-60%, and purified drinking water and feed were freely consumed.

In the acute alcohol animal model, 50 and 200 mg/kg of the example extracts were administered to rats fasted for 18 hours, and 3 g/kg of 30% ethanol was administered 30 minutes after administration, and then 1 ml of blood was collected from the jugular vein at each time point.

Experimental groups are (a) control group, (b) ethanol group, (c) ethanol administration group after administration of 50 mg/kg of the example extract (Example 50 + ethanol group), (d) ethanol administration group after administration of 200 mg/kg of the example extract (Example 200 + ethanol group) was divided into a total of 4 groups.

The blood collection time was five times: before administration of the example extract (0 min), 30 minutes, 60 minutes, 90 minutes, and 300 minutes after administration of ethanol.

2-2. Experiment process (measurement of acetaldehyde level in blood)

The blood collected from the jugular vein of the animal was centrifuged at 4℃ and 2000 rpm for 30 minutes in a centrifuge (Combi 514R, Hanil Science Co., Ltd.), and then the plasma was separated and blood was analyzed using an acetaldehyde analysis kit (megazyme, K-ACHYD). I measured my acetaldehyde concentration.

2-3.Experiment results

As a result of measuring the level of acetaldehyde in the blood of thyme, at 0 minutes before alcohol administration, the control group was 15.40±3.68 g/ml, the ethanol group was 12.93±3.75 g/ml, the thyme 50 + ethanol group was 13.21±3.44 g/ml, and the thyme 200 + Ethanol group was 13.47 ± 3.33 g / ㎖, there was no difference in acetaldehyde level between groups. At 30 minutes after alcohol administration, the control group was 12.35±2.14 g/ml, the ethanol group was 21.51±4.15 g/ml, the thyme 50 + ethanol group was 18.85±3.43 g/ml, and the thyme 200 + ethanol group was 19.90±4.62 g/ml. As a result, the ethanol-administered group showed higher acetaldehyde levels than the control group. At 60 minutes after alcohol administration, the control group was 16.00 ± 3.83 g / ml, the ethanol group was 23.91 ± 4.05 g / ml, the thyme 50 + ethanol group was 19.08 ± 2.60 g / ml, the thyme 200 + ethanol group was 11.30 ± 3.53 g / ml As a result, the thyme-treated group showed lower acetaldehyde levels than the ethanol-only group. At 90 minutes after alcohol administration, the control group was 17.23±2.38 g/ml, the ethanol group was 17.95±3.78 g/ml, the thyme 50 + ethanol group was 15.60±5.10 g/ml, and the thyme 200 + ethanol group was 8.43±1.64 g/ml. The thyme-treated group showed lower acetaldehyde levels than the ethanol-only group. At 300 minutes after alcohol administration, the control group was 11.65±1.19 g/ml, the ethanol group was 18.11±1.58 g/ml, the thyme 50 + ethanol group was 12.10±1.86 g/ml, and the thyme 200 + ethanol group was 14.77±2.39 g/ml. As a result, the thyme-treated group showed lower acetaldehyde levels than the ethanol-only group.

At 90 minutes after alcohol administration, it was observed that the thyme 200 + ethanol group significantly decreased compared to the ethanol group. In addition, it was observed that there was a significant difference between the groups in blood acetaldehyde concentration due to alcohol administration. (See Table 3) In the blood acetaldehyde concentration efficacy evaluation experiment using an acute alcohol animal model of thyme, it was confirmed that there was a significant difference in blood acetaldehyde concentration between groups due to alcohol administration, and thyme at 90 minutes of alcohol administration The concentration of acetaldehyde in the 200 mg/kg administration group was significantly decreased compared to the ethanol group (FIG. 1).

Results of measuring the effect of thyme on the concentration of acetaldehyde in blood (g / ml) 0min 30min 60min 90min 300min control group 15.40±3.68 12.35±2.14b ^,* 16.00± ^3.83b 17.23±2.38 ^a 11.65±1.19 ^c,** ethanol group 12.93±3.75 21.51±4.15 ^a 23.91±4.05 ^a 17.95 ± 3.78 ^a 18.11±1.58 ^a Thyme 50 + Ethanol Group 13.21±3.44 18.85± ^3.43a 19.08± ^2.60b 15.60±5.10 ^a 12.10±1.86 ^c,** Thyme 200 + Ethanol group 13.47±3.33 19.90± ^4.62a 11.30±3.53 ^c,** 8.43±1.64b ^,* 14.77±2.39b ^,*

statistical analysis

Data were expressed as mean ± SD, and statistical analysis was performed using software (SPSS Inc., Chicago, IL, USA). * p <0.05, ** p <0.001 were considered statistically significant.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to limit them, but only to be specifically described.

Formulation Example 1. Preparation of powder

Extract (TQ) ------------------------------------------ 20 mg

Lactose ------------------------------------------------- -- 100 mg

Talc ------------------------------------------------- --- 10mg

A powder is prepared by mixing the above ingredients and filling them in an airtight bag.

Formulation Example 2. Preparation of tablets

Extract (TQ) ----------------------------- 10 mg

Corn Starch -------------------------------------------- 100 mg

Lactose ------------------------------------------------- - 100mg

Magnesium Stearate ------------------------------------- 2 mg

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.

Formulation Example 3. Preparation of capsule formulation

Extract (TQ) ------------------------------------------- 10 mg

Crystalline Cellulose --------------------------------------- 3 mg

Lactose --------------------------------------------- 14.8 mg

Magnesium stearate --------------------------------- 0.2 mg

Capsules are prepared by mixing the above ingredients and filling them into gelatin capsules according to a conventional capsule preparation method.

Formulation Example 4. Preparation of injection

Extract (TQ) ------------------------------------------ 10 mg

Mannitol ------------------------------------------------ 180 mg

Sterile distilled water for injection ----------------------------------- 2974 mg

Na ₂ HPO ₄ ,12H2O ------------------------------------------- 26 mg

It is prepared with the above component content per 1 ampoule (2 ml) according to the conventional method for preparing injections.

Formulation Example 5. Preparation of liquid formulation

Extract (TQ) ------------------------------------------- 20 mg

Iseonghwadang ------------------------------------------------ - 10 g

Mannitol ------------------------------------------------- --- 5g

Purified water ------------------------------------------------- -- Appropriate amount

According to the conventional liquid preparation method, each component is dissolved in purified water, lemon flavor is added in an appropriate amount, the above components are mixed, and then purified water is added to adjust the total volume to 100 ml, and then filled in a brown bottle. Sterilize to prepare a liquid formulation.

Formulation Example 6. Manufacture of health food

Extract (TQ) ------------------------------------------- 1000 mg

Vitamin Blend -------------------------------------------- Appropriate amount

Vitamin A Acetate ------------------------------------- 70 μg

Vitamin E ----------------------------------------------- 1.0 mg

Vitamin B1 --------------------------------------------- 0.13 mg

Vitamin B2 --------------------------------------------- 0.15 mg

Vitamin B6 ---------------------------------------------- 0.5 mg

Vitamin B12 --------------------------------------------- 0.2 μg

Vitamin C ------------------------------------------------ 10 mg

Biotin ------------------------------------------------- - 10 μg

Nicotinamide ----------------------------------------- 1.7 mg

Folic acid ------------------------------------------------- --- 50 μg

Calcium Pantothenate ------------------------------------------ 0.5 mg

Mineral mixture -------------------------------------------- Appropriate amount

Ferrous sulfate --------------------------------------------- 1.75 mg

Zinc Oxide ---------------------------------------------- 0.82 mg

Magnesium Carbonate ------------------------------ 25.3 mg

Potassium Phosphate Monobasic --------------------------------------------- 15 mg

Dibasic Calcium Phosphate --------------------------------------------- 55 mg

Potassium Citrate ---------------------------------------------- 90 mg

Calcium Carbonate ----------------------------------------------------------- 100 mg

Magnesium Chloride ------------------------------------------ 24.8 mg

Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for relatively healthy food in a preferred embodiment, the mixing ratio may be arbitrarily modified. , Granules can be prepared and used in the preparation of health food compositions according to conventional methods.

Formulation Example 7. Manufacture of health drink

Extract (TQ) ------------------------------ 1000mg

Citric acid ------------------------------------------------- 1000mg

oligosaccharide ------------------------------------------------- 100 grams

Plum concentrate ------------------------------------------------ - 2 g

Taurine ------------------------------------------------- ----1 g

Add purified water ------------------------ total 900 ml

After mixing the above components according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2-liter container, sealed and sterilized, and then refrigerated according to the present invention. It is used in the manufacture of health beverage compositions.

Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

Claims (7)

The dried thyme (Thymus quinquecostatus Celak) outpost was washed and cut, mixed with water several times, and then subjected to hot water extraction 2 to 10 times at a temperature of 70 ° C to 120 ° C for 1 hour to 12 hours. The obtained extract was filtered and reduced pressure A pharmaceutical composition for relieving hangover comprising, as an active ingredient, a water extract of thyme spruce obtained through a manufacturing method of concentrating and drying. delete The dried thyme (Thymus quinquecostatus Celak) outpost was washed and cut, mixed with water several times, and then subjected to hot water extraction 2 to 10 times at a temperature of 70 ° C to 120 ° C for 1 hour to 12 hours. The obtained extract was filtered and reduced pressure A health functional food for preventing or alleviating hangovers containing, as an active ingredient, a water extract of thyme spruce obtained through a manufacturing method of concentrating and drying. delete The dried thyme (Thymus quinquecostatus Celak) outpost was washed and cut, mixed with water several times, and then subjected to hot water extraction 2 to 10 times at a temperature of 70 ° C to 120 ° C for 1 hour to 12 hours. The obtained extract was filtered and reduced pressure A health supplement for relieving hangovers containing, as an active ingredient, the water extract of thyme spruce obtained through a manufacturing method of concentrating and drying.
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