KR101001159B1 - Composition for preventing or improving the diabete containing eriobotrya japonica extract - Google Patents
Composition for preventing or improving the diabete containing eriobotrya japonica extract Download PDFInfo
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- KR101001159B1 KR101001159B1 KR1020100054550A KR20100054550A KR101001159B1 KR 101001159 B1 KR101001159 B1 KR 101001159B1 KR 1020100054550 A KR1020100054550 A KR 1020100054550A KR 20100054550 A KR20100054550 A KR 20100054550A KR 101001159 B1 KR101001159 B1 KR 101001159B1
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- Prior art keywords
- extract
- loquat
- composition
- diabetes
- preventing
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/328—Foods, ingredients or supplements having a functional effect on health having effect on glycaemic control and diabetes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
Description
본 발명은 당뇨병 치료 또는 예방용 조성물에 관한 것이다.The present invention relates to a composition for treating or preventing diabetes.
당뇨병은 체내 인슐린 감수성과 인슐린 분비의 균형이 깨어지는 경우, 즉 인슐린이 결핍되거나 인슐린 감수성이 저하되어 혈중의 당분을 이용하지 못해 혈당 조절이 되지 아니하여 소변에 포도당을 배출하는 질환을 의미한다.Diabetes refers to a disorder in which the balance between insulin sensitivity and insulin secretion in the body is broken, that is, insulin deficiency or insulin sensitivity is reduced and glucose is released into the urine because blood glucose is not controlled due to inability to use blood sugar.
상기 당뇨병은 크게 제1형 당뇨병과 제2형 당뇨병으로 구분된다. 상기 제1형 당뇨병 즉, '인슐린 의존형 당뇨병'은 인슐린을 분비하는 췌장 베타 세포(β-cell)의 손상으로 인하여 인슐린의 생산과 분비가 감소하여 발생한다. 상기 제1형 당뇨병을 치료하기 위해서는 일반적으로 외부에서 인슐린을 투여하여 혈당을 낮추고 있다. 상기 제2형 당뇨병 즉, '인슐린 비의존형 당뇨병'은 혈당이 정상 이상으로 높아진 상태를 말하며, 근육, 지방 및 간 등의 말초조직에서 인슐린 작용의 저하와 췌장에서의 인슐린 분비의 균형이 깨어졌을 때 발생한다. 대부분의 당뇨병 환자는 제2형 당뇨병으로 진행되기 이전에 인슐린 저항성과 고인슐린 혈증을 나타낸다. 즉, 제2형 당뇨병은 초기 혈당을 낮추는 인슐린의 작용이 저하되어, 말초조직에서 당을 효과적으로 흡수하지 못하고 에너지로 활용하지 못하는 인슐린 저항성이 발생되며, 이로 인해 유발되는 고혈당을 낮추기 위해 인슐린 분비량이 증가되는 고인슐린 혈증이 나타난다. 이를 극복하지 못하면 결국 제2형 당뇨병으로 진행된다. 우리나라의 경우, 최근 10년 동안 제2형 당뇨병 환자의 수가 증가되고 있다.The diabetes is largely divided into
당뇨병 유병률이 증가하는 원인은 산업화에 따른 경제성장과 식생활 및 생활습관의 변화, 인종이나 가족력 요소와 비만율 증가 등에 의한 것으로 해석되고 있다.The increase in diabetes prevalence is attributed to economic growth, changes in dietary and lifestyle habits, and increased ethnic and family history factors and obesity rates.
당뇨환자의 경우 식이나 약물을 통한 정상혈당의 유지가 중요하다. 그러나 제2형 당뇨의 경우 식이 및 운동을 통한 비 약물적 치료가 시행되더라도 혈당조절이 용이치 않은 경우가 있으며 이때는 경구용 혈당강하제나 인슐린의 투여가 요구된다. 그러나 많이 사용되고 있는 경구용 혈당강하제의 경우 베타세포의 탈진으로 인한 부작용으로 저혈당을 유발될 수 있고 유산증(lactic acidosis)과 같은 부작용이 발생하기도 한다. 또한 인슐린 주사는 사용이 불편하고 저혈당의 위험이 있으면 장기간 사용했을 때 비만이 촉진되어 장기적인 혈당 관리에 어려움을 줄 수 있다. 따라서, 제2형 당뇨병 환자에게 부작용이 적고 투약이 쉬운 천연유래 항당뇨 소재를 개발하고자 하는 요구가 증가되고 있다.In diabetics, it is important to maintain normal blood sugar through diet or medication. However, in case of
본 발명은 상기와 같은 종래기술의 문제점을 해결하기 위한 것으로서, 본 발명은 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물을 제공하는 것을 목적으로 한다.The present invention is to solve the problems of the prior art as described above, it is an object of the present invention to provide a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient.
본 발명은 상기 목적을 달성하기 위하여 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물을 제공한다.The present invention provides a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient in order to achieve the above object.
또한, 상기 목적을 달성하기 위하여, 본 발명은 비파 추출물을 유효성분으로 포함하는 당뇨병 개선 또는 예방용 식품조성물을 제공한다. 본 발명의 식품 조성물의 예로는 식품, 식품첨가제, 음료 또는 음료첨가제를 들 수 있다.In addition, to achieve the above object, the present invention provides a food composition for improving or preventing diabetes comprising a loquat extract as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.
이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명의 발명자들은 부작용이 적고 투약이 쉬운 천연물질 유래 항당뇨 소재에 대하여 연구하던 중, 비파 추출물이 혈당 및 당화헤모글로빈 양을 강하시킬 수 있다는 것을 확인하였으며, 이를 토대로 본 발명을 완성하게 되었다.The inventors of the present invention, while studying a natural substance-derived antidiabetic material with less side effects and easy to administer, it was confirmed that loquat extract can lower the amount of blood sugar and glycated hemoglobin, based on which the present invention was completed.
본 발명에 있어서, 비파(Eriobotrya japonica)는 장미과 상록소교목에 속하는 식물로, 가지가 굵고 잎 뒷면과 더불어 연한 노란빛을 띤 갈색 털이 빽빽이 난다. 잎은 어긋나고 거꾸로 세운 듯한 넓은 바소꼴이며, 가장자리에 이 모양 톱니가 있고, 열매는 구형 또는 타원형으로 식용으로 사용된다. 비파는 기존 민간용법에서 청폐, 진해, 거담, 패열해소 또는 부종 등에 효능이 있는 것으로 알려져 있다. 상기 비파는 바람직하게는 비파 씨 또는 비파 잎, 더욱 바람직하게는 비파 씨일 수 있다. 상기 비파 씨의 경우, 3주 동안 비파 씨 추출물을 투여한 경우, 혈당이 대조군의 절반 이하의 수치로 떨어뜨리고, 당화헤모글로빈의 양도 대조군 뿐만 아니라 비파 잎 추출물에 비하여 현저하게 감소시킬 뿐만 아니라, 대조군과 비교하여 비파 잎 추출물에 비하여 약 2배 이상의 인슐린 분비 증가 효과를 나타내어 혈당 및 인슐린 분비의 측면에서 당뇨병 치료 및 예방을 위한 현저한 효과가 있는 것으로 확인되었다.In the present invention, loquat ( Eriobotrya japonica ) is a plant belonging to the rose family Evergreen arboreal, with thick branches and dense yellowish brown hairs along with the back of leaves. Leaves are alternate, upside-down, broad-shaped bar-shaped, with serrated teeth on the edges, and fruit is spherical or oval, used for food. Loquat is known to be effective in clearing, Jinhae, expectoration, septic breakdown or edema in conventional civilian use. The loquat may preferably be loquat seed or loquat leaf, more preferably loquat seed. In the case of loquat seed, when the loquat seed extract was administered for 3 weeks, blood sugar dropped to less than half of the control group, and the amount of glycated hemoglobin was significantly reduced in comparison with the loquat leaf extract as well as the control group. Compared with loquat leaf extract, it showed an effect of increasing insulin secretion about 2 times or more, and thus, it was confirmed that there was a remarkable effect for treating and preventing diabetes in terms of blood glucose and insulin secretion.
본 발명의 추출물은 추출용매로 추출하거나 추출용매로 추출하여 제조한 조추출물에 분획용매를 가하여 분획하여 제조할 수 있다.The extract of the present invention can be prepared by extracting with an extraction solvent or by adding a fractional solvent to the crude extract prepared by extraction with an extraction solvent.
상기 추출용매는 물 및 유기용매로 이루어진 군에서 선택된 1종 이상일 수 있다. 상기 유기용매는 메탄올, 에탄올 등의 탄소수 1 내지 5의 알코올, 에틸아세테이트 또는 아세톤 등의 극성용매와 에테르, 클로로포름, 벤젠, 헥산 또는 디클로로메탄의 비극성용매 또는 이들의 혼합용매일 수 있다.The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as alcohol having 1 to 5 carbon atoms such as methanol or ethanol, ethyl acetate or acetone, and a nonpolar solvent of ether, chloroform, benzene, hexane or dichloromethane, or a mixed solvent thereof.
본 발명의 추출용매는 바람직하게는 물 및 탄소수 1 내지 4의 알코올로 이루어진 군에서 선택된 1종 이상의 용매일 수 있고, 더욱 바람직하게는 50 내지 99%의 에탄올 등의 탄소수 1 내지 4의 알코올 수용액, 더더욱 바람직하게는 70 내지 95% 에탄올 등의 탄소수 1 내지 4의 알코올 수용액일 수 있다.The extraction solvent of the present invention may be at least one solvent selected from the group consisting of water and alcohols having 1 to 4 carbon atoms, more preferably an aqueous solution of alcohols having 1 to 4 carbon atoms such as 50 to 99% ethanol, Even more preferably, it may be an aqueous solution of alcohol having 1 to 4 carbon atoms such as 70 to 95% ethanol.
본 발명의 추출물은 통상의 육상 식물, 바람직하게는 잎 또는 씨앗을 추출대상으로 한 추출물의 제조방법에 따라 제조된 것일 수 있으며, 구체적으로는 냉침추출법, 온침추출법 또는 열 추출법 등일 수 있으며, 통상의 추출기기, 초음파분쇄 추출기 또는 분획기를 이용할 수 있다.The extract of the present invention may be prepared according to a conventional land plant, preferably a method of preparing an extract for extracting leaves or seeds, and specifically, may be cold extraction, hot extraction or thermal extraction, and the like. Extraction apparatus, ultrasonic grinding extractor or fractionator can be used.
또한, 본 발명의 추출물은 상기 용매로 추출한 조추출물에 대하여 분획용매를 가한 후, 분획과정을 더욱 실시한 분획물일 수 있다. 상기 분획용매는 에틸아세테이트, 에테르, 클로로포름, 벤젠, 헥산, 메틸렌클로라이드 및 이들의 혼합용매로 이루어진 군에서 선택된 용매일 수 있으며, 바람직하게는 헥산일 수 있다. 상기 분획용매 중, 헥산 분획용매로 제조된 분획물의 경우, 시험관내 실험을 통하여 DPP-IV 활성 억제여부를 측정한 결과, 다른 분획용매인 에틸아세테이트나 물을 분획용매로 이용한 경우에 비하여 헥산분획의 억제효율이 동일 농도의 경우 약 350% 내지 400%의 억제효율을 갖고, 헥산 분획 250㎍/mL의 함량으로도 에틸아세테이트 분획 또는 물 분획 1,000㎍/mL을 처리한 경우와 유사한 정도의 억제효율을 나타내는 것으로 확인되어, 상기 헥산 분획의 경우 현저하게 우수한 효과를 갖는 것으로 확인되었다.In addition, the extract of the present invention may be a fraction further subjected to the fractionation process after adding a fractional solvent to the crude extract extracted with the solvent. The fractional solvent may be a solvent selected from the group consisting of ethyl acetate, ether, chloroform, benzene, hexane, methylene chloride and mixed solvents thereof, preferably hexane. Among the fractionated solvents, fractions prepared with hexane fractionation solvents were tested for inhibition of DPP-IV activity through in vitro experiments. As a result, fractions of hexane fractions were compared with those of other fractionation solvents, ethyl acetate or water. At the same concentration, the inhibitory efficiency was about 350% to 400%, and the inhibitory efficiency was similar to that of the ethyl acetate fraction or the water fraction 1,000㎍ / mL with the
상기 제조된 추출물 또는 상기 분획과정을 수행하여 수득한 분획물은 이후 여과하거나 농축 또는 건조과정을 수행하여 용매를 제거할 수 있으며, 여과, 농축 및 건조를 모두 수행할 수 있다. 구체적으로 상기 여과는 여과지를 이용하거나 감압여과기를 이용할 수 있으며, 상기 농축은 감압 농축기, 일예로 회전 증발기를 이용하여 감압 농축할 수 있으며, 상기 건조는 일예로 동결건조법으로 수행할 수 있다.The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.
본 발명의 일예로, 상기 비파 추출물은 하기 방법에 의해 수득될 수 있다.In one embodiment of the present invention, the loquat extract may be obtained by the following method.
비파 잎과 비파 씨를 80% 메탄올을 이용하여 48시간 추출하고, 감압여과의 방식으로 상층액만을 분리하여 제조할 수 있고, 상기 제조된 추출액은 50℃의 수욕상에서 감압농축하고, -70℃의 냉동고에서 24시간 동안 동결하여 건조시킬 수 있다.Loquat leaves and loquat seeds can be extracted with 80% methanol for 48 hours, and can be prepared by separating only the supernatant by vacuum filtration. The extract is concentrated under reduced pressure in a water bath at 50 ° C., and a freezer at −70 ° C. Freeze-dried for 24 hours at.
본 발명은 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물에 관한 것이다.The present invention relates to a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient.
본 발명에 있어서, 당뇨병은 체내 인슐린 감수성과 인슐린 분비의 균형이 깨어지는 경우, 즉 인슐린이 결핍되거나 인슐린 감수성이 저하되어 혈중의 당분을 이용하지 못해 혈당 조절이 되지 아니하여 소변에 포도당을 배출하는 질환을 의미한다. 상기 당뇨병은 크게 제1형 당뇨병과 제2형 당뇨병으로 구분되며, 상기 당뇨병은 바람직하게는 제2형 당뇨병일 수 있다.In the present invention, diabetes is a disease in which the balance between insulin sensitivity and insulin secretion in the body is broken, that is, insulin deficiency or insulin sensitivity is reduced so that glucose cannot be released into the urine because blood sugar is not controlled because blood sugar is not used. Means. The diabetes is largely divided into
본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 조성물 총 중량에 대하여 상기 비파 추출물을 0.001 내지 99.99중량%, 바람직하게는 0.1 내지 99 중량%로 포함할 수 있다.Diabetic treatment or prevention composition comprising a loquat extract of the present invention as an active ingredient may comprise 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight of the loquat extract based on the total weight of the composition.
상기 비파 추출물, 바람직하게는 비파 씨 추출물, 더욱 바람직하게는 비파 씨 알코올 추출물의 헥산 분획물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 천연물질의 추출물이라는 점에서 부작용의 문제가 발생되지 아니하고, 당뇨병의 개선 또는 예방 효과가 있다는 점에서 우수한 효과를 가진다.The loquat extract, preferably a loquat seed extract, more preferably a composition for treating or preventing diabetes comprising the hexane fraction of the loquat seed alcohol extract as an active ingredient does not cause problems of side effects in that it is an extract of natural substances, It has an excellent effect in that it can improve or prevent diabetes.
본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 인간에 직접 적용될 수 있다.Diabetic treatment or prevention composition comprising a loquat extract of the present invention as an active ingredient can be applied directly to humans.
상기 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 상기 비파 추출물을 유효성분으로 단독으로 포함할 수 있고, 이외 제형, 사용방법 및 사용목적에 따라 추가성분 즉, 약제학적으로 허용되거나 영양학적으로 허용되는 담체, 부형제, 희석제 또는 부성분을 추가로 포함할 수 있다.Diabetic treatment or prevention composition comprising the loquat extract as an active ingredient may comprise the loquat extract alone as an active ingredient, other ingredients, pharmaceutically acceptable or nutritious according to the formulation, method and purpose of use. It may further comprise a pharmaceutically acceptable carrier, excipient, diluent or accessory.
보다 상세하게는, 상기 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 상기 유효성분 외에 추가로 영양제, 비타민, 전해질, 풍미제, 착색제, 중진제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 추가로 함유할 수 있다. 또한, 상기 담체, 부형제 또는 희석제는 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 자이리톨, 에리스리톨, 말티톨, 전분, 아키시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로오스, 메틸 셀룰로오스, 미정질 셀루로오스, 폴리비닐 피롤리돈, 물, 메틸하이드록시벤조에이트, 프로필하이드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유, 덱스트린, 칼슘카보네이드, 프로필렌글리콜, 리퀴드 파라핀, 생리식염수로 이루어진 군에서 선택된 1이상 일 수 있으나, 이에 한정되는 것은 아니며 통상의 담체, 부형제 또는 희석제 모두 사용가능하다. 상기 성분들은 상기 유효성분인 비파 추출물에 독립적으로 또는 조합하여 추가될 수 있다.In more detail, the composition for treating or preventing diabetes comprising the loquat extract as an active ingredient may further include nutrients, vitamins, electrolytes, flavors, coloring agents, neutralizing agents, pectic acid and salts thereof, alginic acid and the like in addition to the active ingredient. Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the carrier, excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, acycia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline In the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, saline It may be one or more selected, but is not limited to any conventional carrier, excipient or diluent can be used. The ingredients may be added independently or in combination with the loquat extract, which is the active ingredient.
상기 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 조성물 총 중량에 대하여 상기 비파 추출물을 0.001 중량% 내지 99.9 중량%, 바람직하게는 0.1 중량% 내지 99 중량%, 더욱 바람직하게는 1중량% 내지 50 중량% 포함할 수 있다. 또한, 상기 추가성분의 함량은 바람직하게는 상기 당뇨병 치료 또는 예방용 조성물 100 중량부 당 0.1 내지 20 중량부 범위에서 추가할 수 있다.The composition for treating or preventing diabetes comprising the loquat extract as an active ingredient is 0.001 wt% to 99.9 wt%, preferably 0.1 wt% to 99 wt%, more preferably 1 wt% of the loquat extract, based on the total weight of the composition. % To 50% by weight. In addition, the content of the additional component is preferably added in the range of 0.1 to 20 parts by weight per 100 parts by weight of the composition for treating or preventing diabetes.
또한, 상기 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 약제화하는 경우, 통상의 충진제, 증량제, 결합제, 붕해제, 계면활성제, 항응집제, 윤활제, 습윤제, 향료, 유화제 또는 방부제 등을 더욱 포함할 수 있으며, 경구 또는 비경구 모두 사용 할 수 있다.In addition, the composition for treating or preventing diabetes comprising the loquat extract as an active ingredient, when formulated, common fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, flavors, emulsifiers or preservatives, etc. It may further include, can be used both oral or parenteral.
구체적으로 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 상기 비파 추출물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트(calcium carbonate), 수크로스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제된다. 또한, 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데, 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제 예를 들면, 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다.Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, water, and the like in the loquat extract. Prepared by mixing sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may include, for example, wetting agents, sweeteners, fragrances, and preservatives. have.
또한, 본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물의 제형은 사용방법에 따라 바람직한 형태일 수 있으며, 특히 포유동물에 투여된 후 활성 성분의 신속, 지속 또는 지연된 방출을 제공할 수 있도록 당업계에 공지된 방법을 채택하여 제형화할 수 있다. 구체적인 제형의 예로는 과립제, 산제, 시럽제, 액제, 현탁제, 정제, 주사제, 주정제, 카타플라스마제(cataplasma), 캅셀제, 연질 또는 경질 젤라틴 캅셀 등이 있다.In addition, the formulation of the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may be in a preferred form depending on the method of use, and in particular, provides rapid, sustained or delayed release of the active ingredient after administration to a mammal. It can be formulated by adopting a method known in the art to do so. Examples of specific formulations include granules, powders, syrups, solutions, suspensions, tablets, injections, spirits, cataplasma, capsules, soft or hard gelatin capsules, and the like.
더 나아가 본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 당해 기술 분야의 공지된 적절한 방법을 사용하여 또는 레밍턴의 문헌(Remington's Pharmaceutical Science(최근판), Mack Publishing Company, Easton PA)에 개시되어 있는 방법을 이용하여 바람직하게 제형화될 수 있다.Furthermore, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may be prepared using Remington's Pharmaceutical Sciences (Remington's Pharmaceutical Science, recent edition), Mack Publishing Company, Easton PA using appropriate methods known in the art. Formulated preferably using the method disclosed herein.
본 발명에 따른 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물의 투여량은, 투여방법, 복용자의 연령, 성별 및 체중, 및 질환의 중증도 등을 고려하여 당업자에 의해 적절하게 선택될 수 있다. 일예로, 본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 상기 비파 추출물을 기준으로 할 때, 0.0001 mg/kg 내지 1000 mg/kg으로, 보다 효과적이기 위해서는 0.01 mg/kg 내지 100 mg/kg으로 투여할 수 있다. 투여는 하루에 한번 투여할 수도 있고, 수회 나누어 투여할 수도 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다.The dosage of the composition for treating or preventing diabetes comprising the loquat extract according to the present invention as an active ingredient may be appropriately selected by those skilled in the art in consideration of the method of administration, the age, sex and weight of the recipient, and the severity of the disease. have. For example, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient is 0.0001 mg / kg to 1000 mg / kg, based on the loquat extract, in order to be more effective, from 0.01 mg / kg to It may be administered at 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.
또한, 본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은, 상기 비파 추출물 이외에 공지의 당뇨병 치료 또는 예방 효과를 갖는 화합물 또는 천연물에 대한 추출물을 더욱 포함할 수 있으며, 상기 비파 추출물 100 중량부에 대하여 각각 5 중량부 내지 200 중량부로 포함될 수 있다.In addition, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient, in addition to the loquat extract may further include an extract for a compound or natural product having a known diabetes treatment or prevention effect, the
또한, 상기 목적을 달성하기 위하여, 본 발명은 상기 비파 추출물을 유효성분으로 포함하는 당뇨병 예방 또는 개선용 식품 조성물을 제공한다.In addition, in order to achieve the above object, the present invention provides a food composition for preventing or improving diabetes comprising the loquat extract as an active ingredient.
본 명세서에서 식품이란 함은 영양소를 한 가지 또는 그 이상 함유하고 있는 천연물 또는 가공품을 의미하며, 바람직하게는 어느 정도의 가공 공정을 거쳐 직접 먹을 수 있는 상태가 된 것을 의미하며, 통상적인 의미로서, 건강기능식품, 음료, 식품 첨가제 및 음료 첨가제 등을 모두 포함하는 의도이다.In the present specification, the term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process. It is intended to include all dietary supplements, beverages, food additives and beverage additives.
본 발명의 식품은 예를 들어, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 건강기능식품 등이 있다. 추가로, 본 발명에서 식품에는 특수영양식품(예, 조제유류, 영,유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예, 라면류, 국수류 등), 건강보조식품, 조미식품(예, 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예, 스넥류), 유가공품(예, 발효유, 치즈 등), 기타 가공식품, 김치, 절임식품(각종 김치류, 장아찌 등), 음료(예, 과실,채소류 음료, 두유류, 발효음료류 등), 천연조미료(예, 라면스프 등)을 포함하나 이에 한정되지 않는다. 상기 식품, 건강기능식품, 음료, 식품 첨가제 및 음료 첨가제는 통상의 제조방법으로 제조될 수 있다.Examples of the food of the present invention include various foods, beverages, gums, teas, vitamin complexes, and health functional foods. In addition, the food in the present invention includes special nutritional products (e.g., prepared oils, infants, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( For example, soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruits, vegetable drinks, soy milk, fermented beverages, and the like, and natural seasonings (eg, ramen soup). The food, health functional food, beverages, food additives and beverage additives may be prepared by conventional manufacturing methods.
본 발명에서 건강기능식품이란 식품에 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 식품의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 식품군이나 식품 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 식품을 의미한다.In the present invention, the health functional food is a biological defense rhythm control, disease prevention and the like having a food group or a food composition that has added value to the food by using physical, biochemical, biotechnological techniques, etc. It means a food that is designed and processed to fully express the gymnastics function on recovery.
상기 건강기능식품에는 식품학적으로 허용 가능한 식품 보조 첨가제를 포함할 수 있으며, 건강기능식품의 제조에 통상적으로 사용되는 적절한 담체, 부형제 및 희석제를 더욱 포함할 수 있다.The dietary supplement may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of dietary supplements.
본 발명에서 음료란 갈증을 해소하거나 맛을 즐기기 위하여 마시는 것의 총칭을 의미하며 건강기능음료를 포함하는 의도이다. 상기 음료는 지시된 비율로 필수 성분으로서 상기 비파 추출물을 유효성분으로 포함하는 것 외에 다른 성분에는 특별한 제한이 없으며, 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.In the present invention, the drink refers to a generic term for drinking to quench thirst or to enjoy the taste and is intended to include a functional beverage. The beverage is not particularly limited to other ingredients other than including the loquat extract as an active ingredient in the indicated ratio as an active ingredient, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. .
상기의 천연 탄수화물의 예는 모노사카라이드, 예를 들어 포도당, 과당 등 디사카라이드, 예를 들어 말토스, 수크로스 등 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알코올이다. 상기한 것 이외의 향미제로서 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 식품 조성물 100㎖ 당 일반적으로 약 1 내지 20g, 바람직하게는 5 내지 12g일 수 있다. 그밖에 본 발명의 조성물은 천연 과일 주스, 과일 쥬스 음료, 야채 음료의 제조를 위한 과육을 추가로 함유할 수 있다.Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate may generally be about 1 to 20 g, preferably 5 to 12 g per 100 ml of the food composition of the present invention, in addition to the composition of the present invention for the production of natural fruit juices, fruit juice drinks, vegetable drinks It may further contain pulp for.
상기 외에 본 발명의 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다. 이러한 성분을 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 그렇게 중요하지 않지만, 본 발명의 비파 추출물 100 중량부 당 0 내지 2,000 중량부 범위에서 선택될 수 있다.In addition to the above, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. These components can be used independently or in combination. The proportion of such additives is not so critical, but may be selected in the range of 0 to 2,000 parts by weight per 100 parts by weight of loquat extract of the present invention.
본 발명에서 건강기능음료란 음료에 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 음료의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 음료 군이나 음료 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 음료를 의미한다.In the present invention, the health functional beverage is a biological defense rhythm control or disease prevention of a beverage group or beverage composition which has added value to the beverage by using physical, biochemical and biotechnological techniques to act and express the function of the beverage for a specific purpose. Means a beverage that is designed and processed to fully express the gymnastic function related to recovery and the like.
상기 건강기능음료는 지시된 비율로 필수 성분으로서 본 발명의 비파 추출물을 함유하는 외에는 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.The health functional beverage is not particularly limited to other ingredients except for containing the loquat extract of the present invention as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks.
상기 천연 탄수화물의 예는 모노사카라이드, 예를 들어 포도당, 과당 등 디사카라이드, 예를 들어 말토스, 수크로스 등 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알코올이다. 상기한 것 이외의 향미제로서 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100㎖ 당 일반적으로 약 1 내지 20 g, 바람직하게는 5 내지 12 g이다.Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and xylitol Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.
또한, 비파 추출물을 유효성분으로 포함하는 당뇨병 예방 또는 개선용 식품 조성물에 있어서, 상기 비파 추출물의 양은 전체 식품 중량의 0.01 내지 15 중량%로 포함할 수 있으며, 음료 조성물은 100 ㎖를 기준으로 0.02 내지 5 g, 바람직하게는 0.3 내지 1g의 비율로 포함할 수 있다.In addition, the diabetic preventive or improved food composition comprising a loquat extract as an active ingredient, the amount of the loquat extract may include 0.01 to 15% by weight of the total food weight, the beverage composition is based on 100 ml 0.02 to 5 g, preferably 0.3 to 1g.
본 발명의 비파 추출물은 혈중 혈당 변화, 당화 헤모글로빈 양의 변화 및 인슐린 분비량을 통하여 확인한 결과, 혈당과 당화 헤모글로빈양을 낮추고, 인슐린 분비량을 증가시켜 혈당저하 효능을 포함한 당뇨병 치료 또는 예방 효과를 보이며, 특히 비파의 부위 중 비파 씨 추출물의 경우 비파 잎 추출물에 비해 현저한 혈당 저하 효과 및 인슐린 분비량 증가효과를 나타내고, 상기 비파 잎 추출물의 분획물의 경우 헥산을 용매로 한 비파 잎 추출물의 헥산 분획물은 다른 분획용매를 사용하는 것에 비하여 현저하게 우수한 DPP-IV 활성 억제효과를 나타내어 혈당 저하 효과를 갖는 것으로 확인되었다.The loquat extract of the present invention has been confirmed through the change in blood glucose, the amount of glycated hemoglobin and the amount of insulin secreted, lowers the amount of glucose and glycated hemoglobin, and increases the amount of insulin secreted to treat or prevent diabetes, including hypoglycemic effects, in particular Loquat seed extract of loquat seed extract showed significantly lower blood sugar and insulin secretion effect compared to loquat leaf extract, and the hexane fraction of loquat leaf extract using hexane as the fraction of loquat leaf extract showed different fraction solvent. It was confirmed to have a blood glucose lowering effect by showing a remarkably superior inhibitory effect of DPP-IV activity compared to the use.
상술한 바와 같이, 본 발명의 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물의 섭취는 당화 헤모글로빈양을 감소시키면서 혈당을 강하시킬 수 있고, 인슐린 분비량을 증가시킬 수 있어서, 당뇨병의 치료 또는 예방에 효과가 있을 뿐만 아니라, 인공 화합물질인 기존의 당뇨병 치료제와 달리 천연물질인 천일염은 부작용 등이 문제되지 아니하고, 나아가 우리나라 특히, 전라남도에서 풍부하게 생산되는 특화자원인 비파를 이용하는 것이므로 경제적으로도 유리하여, 비파 추출물을 유효성분으로 포함하는 당뇨병 치료 또는 예방용 조성물은 산업적으로 그 효과가 매우 크다 할 것이다.As described above, ingestion of the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may lower blood sugar while increasing the amount of glycated hemoglobin, and increase the amount of insulin secreted, thereby treating diabetes or Not only is it effective in preventing, and unlike the existing anti-diabetic drugs, which are artificial compounds, natural salt, natural salt does not have any side effects, and furthermore, it is economically economical because it uses specialized resources, abundantly produced in Korea, especially Jeollanam-do. Advantageously, the composition for treating or preventing diabetes containing loquat extract as an active ingredient will be very effective industrially.
도 1은 본 발명의 일 실시예에 따른 실험동물의 사육기간 동안 식이의 섭취량의 변화를 나타낸 그래프이다.
도 2는 본 발명의 일 실시예에 따른 실험동물의 사육기간 동안 물의 섭취량의 변화를 나타낸 그래프이다.1 is a graph showing a change in the intake of the diet during the breeding period of the experimental animal according to an embodiment of the present invention.
2 is a graph showing a change in water intake during the breeding period of the experimental animal according to an embodiment of the present invention.
이하, 본 발명의 바람직한 실시예를 기재한다. 하기의 실시예는 본 발명을 예시하기 위한 것일 뿐 본 발명의 권리범위가 하기 실시예에 의해 한정되는 것은 아니다.
Hereinafter, preferred embodiments of the present invention are described. The following examples are only for illustrating the present invention and the scope of the present invention is not limited by the following examples.
[실시예][Example]
실시예 1: 비파 추출물의 제조Example 1 Preparation of Loquat Extract
대한민국 목포대학교 약초원으로부터 수득한 비파 잎과 완도에서 채취한 비파 씨를 80% 메탄올로 48시간 추출하고, 감압여과의 방식으로 상층액만을 분리하여 각각 비파 잎 추출액과 비파 씨 추출액을 제조하였다. 제조된 추출액은 50℃의 수욕상에서 감압농축하고, -70℃의 냉동고에서 24시간 동안 동결하고, 동결건조기(freeze dryer system)를 이용하여 건조시킨 후 비파 추출물 시료를 제조하였다.Loquat leaves obtained from Mokpo National University herbal medicine and loquat seeds collected from Wando were extracted with 80% methanol for 48 hours, and the supernatant was separated by vacuum filtration to prepare loquat leaf extract and loquat seed extract, respectively. The prepared extract was concentrated under reduced pressure in a water bath at 50 ° C., frozen in a freezer at −70 ° C. for 24 hours, dried using a freeze dryer system, and a loquat extract sample was prepared.
비파 씨 메탄올 추출물의 분획물은 상기 조 추출물 30g을 10배의 증류수 즉, 300g의 증류수로 치환한 후, 동일한 양의 헥산(hexane)을 첨가하고 분획하여 헥산 층만을 분리함으로써 헥산 분획액을 제조하고, 나머지 용액(물층)에 동일한 양의 증류수와 에틸아세테이트를 첨가하고 분획하여 에틸아세테이트 층만을 분리함으로써 에틸아세테이트 분획액을 제조하고, 나머지 용액을 물(water) 분획액으로 사용하였다. 상기 분획을 통해 제조된 각 용매별 등골나물 분획액을 각각 감압농축기를 이용하여 감압 농축한 뒤, -70℃에서 동결하고, 동결건조기를 이용하여 건조시킨 후, 비파 분획물 시료를 제조하였다.Fraction of Vipa seed methanol extract was prepared by replacing 30 g of the crude extract with 10-fold distilled water, that is, 300 g of distilled water, and then adding the same amount of hexane and fractionating to separate only the hexane layer, thereby preparing a hexane fraction. The same amount of distilled water and ethyl acetate were added to the remaining solution (water layer) and fractionated to separate only the ethyl acetate layer to prepare an ethyl acetate fraction, and the remaining solution was used as the water fraction. The spinach sprout fractions for each solvent prepared through the fractions were concentrated under reduced pressure using a vacuum concentrator, and then frozen at -70 ° C, dried using a lyophilizer, and then a loquat fraction sample was prepared.
상기 수득한 추출물 시료 및 분획물 시료는 실험에 사용하기 전까지 냉동고를 이용하여 -20℃에서 보관하였다.
The obtained extract sample and fraction sample were stored at −20 ° C. using a freezer until use in experiments.
실시예 2: 비파 추출물의 당뇨병 치료 효과 측정Example 2 Determination of Diabetic Therapeutic Effect of Loquat Extract
제2형 당뇨 동물 모델(C57BL/Ks db/db mice)인 실험용 쥐를 이용하여 실시예 1에서 제조한 비파 추출물의 당뇨병 치료 효과를 측정하였다.
The anti-diabetic effect of the loquat extract prepared in Example 1 was measured using a laboratory mouse of
2-1. 실험동물의 사육 2-1. Breeding of Experimental Animals
실험동물은 6주령 수컷의 db/db 비만 마우스(C57BL/Ks db/db mice)를 (주)Harlan에서 공급받아 사육하였다. 실험시작 3일전부터 2018S Teklad Global 18% Protein Rodent Diet(Harlan)로 적응시킨 후, 체중을 기준으로 실험군 당 8마리씩 대조군(Control), 비파씨 추출물(E. japonica seeds) 및 비파잎 추출물(E. japonica leaves)을 먹인 실험군으로 구분하여, 총 3그룹으로 나누어 사육하였다. 추출물 투여량은 200mg/kg 용량으로 매일 같은 시간에(오전 10시) 6주 동안 경구 투여 하였으며, 식이와 물은 매일 신선한 것으로 공급하였고, 동물 사육실은 온도, 습도, 채광을 일정하게 유지하여 6주간 사육하였다.
Experimental animals were fed 6 weeks-old male db / db obese mice (C57BL / Ks db / db mice) from Harlan Co., Ltd .. Adapted to 2018S Teklad Global 18% Protein Rodent Diet (Harlan) three days before the start of the experiment, 8 rats per experimental group, based on body weight, control, E. japonica seeds and E. japonica seeds ( E. Japonica leaves) were divided into three groups and fed into three groups. The dosage of the extract was 200 mg / kg at the same time every day (10 am) for 6 weeks. The diet and water were supplied fresh every day, and the animal breeding room maintained the temperature, humidity and light for 6 weeks. Breeding.
2-2. 식이섭취량 및 수분섭취량 측정2-2. Dietary Intake and Water Intake Measurement
상기 실시예 2-1의 실험동물의 식이섭취량 및 수분섭취량은 전날 공급분에서 남은 양을 빼는 방법으로 매주 1회 측정하였고, 그 결과를 도 1 및 도 2에 나타내었다.The dietary intake and the water intake of the experimental animals of Example 2-1 were measured once a week by subtracting the remaining amount from the feed the day before, and the results are shown in FIGS. 1 and 2.
상기 도 1 및 도 2에 나타낸 바와 같이, 식이섭취량과 물 섭취량의 경우 실험기간 동안 대조군에 비해 비파 추출물을 섭취시킨 실험군에서 감소하는 경향을 보였고, 특히 비파씨 추출물을 섭취시킨 경우에는 대조군에 비해 식이 섭취량 및 물 섭취량이 모두 유의적으로 감소하였다.1 and 2, the dietary intake and water intake showed a tendency to decrease in the experimental group ingested loquat extract compared to the control group during the experimental period, especially intake of the loquat seed extract compared to the control group Both intake and water intake were significantly decreased.
당뇨병 특히, 1형 당뇨병의 경우, 세포가 당을 이용하지 못하므로 다식현상이 유발되고 과잉의 혈당을 배설하기 위하여 다뇨의 현상을 보이며 이로 인하여 갈증이 유발하게 되고 생체내의 수분부족현상을 가져와 심하면 탈수현상이 나타날 수도 있다. 이러한 당뇨병의 증상을 고려하면, 식이섭취량과 물 섭취량이 현저하게 감소된 비파씨 추출물의 경우, 당뇨병 치료 또는 예방 효과가 뛰어날 것으로 기대되었다.
In diabetes mellitus, especially
2-3. 혈당 및 인슐린 양의 측정2-3. Measurement of blood sugar and insulin levels
사육이 끝난 실험동물은 희생 전 12시간 절식시킨 후, 심장에서 헤파린을 처리한 실린지를 이용하여 혈액을 채취하여 일부는 혈당 측정에 사용하였고, 남은 혈액은 3000rpm에서 15분간 원심 분리하여 혈청을 얻은 후 냉동보관 하였으며, 상기 혈청으로부터 혈청 인슐린(insulin) 양을 분석하였다.After the animals were bred for 12 hours before sacrifice, blood was collected by using heparin-treated syringes from the heart, and some were used for blood glucose measurement. The remaining blood was centrifuged at 3000 rpm for 15 minutes to obtain serum. Cryopreservation was performed, and serum insulin (insulin) amount was analyzed from the serum.
상기 채취한 혈액으로부터 혈당의 측정은 추출물을 경구투여한지 3주후 및 희생시 2회 측정하였고, 경구 투여 3주 후에는 꼬리 정맥에서 측정하였고, 마지막 희생시는 심장의 혈액으로부터 측정하였으며, 측정은 혈당계(Medisens Optium)를 이용하여 수행하였다. 또한, 인슐린 양은 상기 분리된 혈청으로부터 마우스용 인슐린 ELISA kit(Shibayagi)를 이용하여 측정하였다.Blood glucose was measured from the collected blood three weeks after oral administration of the extract and two times at sacrifice, at three weeks after oral administration, at the tail vein, and at the last sacrifice, from the blood of the heart. (Medisens Optium). In addition, the amount of insulin was measured using the insulin ELISA kit (Shibayagi) for mice from the separated serum.
상기 측정된 결과를 하기 표 1에 나타내었다. 모든 수치는 최소한 2회 이상 실험을 반복하여 수행하고, 평균치±표준오차로 표시하였고, 각 군과의 비교는 ANOVA test후 사후검증은 Duncan의 다중비교를 사용하였다(p<0.05). The measured results are shown in Table 1 below. All the values were repeated at least two times, and the mean ± standard error was compared with each group. After the ANOVA test, post-test was performed using Duncan's multiple comparison (p <0.05).
(insulin, ng/ml)insulin
(insulin, ng / ml)
(E. japonica seeds)Loquat Seed Extract
( E. japonica seeds)
(E. japonica leaves)Loquat Leaf Extract
( E. japonica leaves)
상기 표 1에 나타낸 바와 같이, 공복시 혈당은 추출물 투여 3주후와 실험 종료시 혈당 모두 대조군에 비해 감소하였다. 특히, 비파씨 추출물의 경우 추출물 투여 3주후 혈당이 대조군의 혈당의 절반 정도로 감소되는 것으로 확인되어, 대조군에 비해 유의적으로 감소됨이 확인되었다. 또한, 당화헤모글로빈도 비파씨와 비파잎 추출물 투여군 모두 대조군에 비하여 감소하였으며, 특히, 비파씨 추출물 투여군은 비파잎 투여군에 비해 약 2배의 감소 효과가 있는 것으로 확인되었다. 또한, 인슐린 양의 경우, 비파 추출물 투여시 인슐린의 양이 증가되는 것으로 확인되어 인슐린 부족과 관련된 당뇨병 치료 효과가 우수할 것으로 기재되었다. 특히, 비파씨 추출물의 경우, 비파잎 추출물에 비하여 인슐린 증가량이 약 2.5배여서 현저하게 우수한 치료 효과가 있을 것으로 기대되었다.
As shown in Table 1, fasting blood glucose was decreased compared to the control group after 3 weeks after the administration of the extract and at the end of the experiment. In particular, in the case of
실시예Example 3: 비파 3: loquat 분획물의Fraction 당뇨병 치료 효과 측정 Diabetes Treatment Measures
상기 실시예 2에서 항당뇨 효과가 우수한 것으로 확인된 비파 씨 알코올 추출물에 대한 분획용매의 종류에 따른 분획물의 당뇨병 치료 효과를 측정하기 위하여, 상기 실시예 1에서 제조된 비파 씨 메탄올 추출물의 3종의 분획물에 대한 DPP-IV 활성을 측정하였다.In order to measure the diabetic therapeutic effect of the fraction according to the type of fraction solvent for the vipa seed alcohol extract confirmed to have excellent anti-diabetic effect in Example 2, three kinds of vipa seed methanol extract prepared in Example 1 DPP-IV activity on the fractions was measured.
상기 DPP-IV 활성은 Cell-free system에서 측정하였으며, 보다 구체적으로 다음과 같은 방법으로 수행하였다.The DPP-IV activity was measured in a Cell-free system, and more specifically, in the following manner.
상기 실시예 2-1의 실험동물인 db/db 비만 마우스(C57BL/Ks db/db mice)를 비파씨 추출물 또는 비파씨 분획물을 섭취시키지 않은 것을 제외하고는 상기 실시예 2-1의 조건 및 방법을 응용하여 사육하였다.The conditions and methods of Example 2-1 except that the db / db obese mice (C57BL / Ks db / db mice), which are the experimental animals of Example 2-1, were not ingested with the vifa seed extract or the vifase fraction. Breeding was applied.
상기 사육이 끝난 실험동물은 희생 전 12시간 절식시킨 후, 마취하고 희생시켰다. 상기 희생시킨 실험동물의 복부를 절개 한 후, 대정맥에서 혈액을 채취한 뒤 곧바로 3000 rpm에서 20분 동안 원심분리하였다. 상기 원심분리를 통해 혈청을 분리하여 실험에 바로 사용하였다. 먼저, 96-well microlplate에 상기 분리한 실험동물의 혈청 10 μl을 넣고 상기 실시예 1에서 제조된 3종의 비파씨 분획물 용액 25 μl를 각각 첨가한 후, 최종 농도가 50 μM인 glycyl-prolyl-7-amino-4-methyl-coumarin(Gly-Pro-AMC)를 25 μl씩 첨가하여 60분 동안 37℃에서 반응시켰다. 상기 비파씨 분획물 용액은 용매에 상기 각각의 비파씨 분획물을 최종 농도가 각각 250 μg/mL, 500 μg/mL 및 1,000 μg/mL이 되도록 조절하여 제조하였다.The breeding animals were fasted 12 hours before sacrifice, anesthetized and sacrificed. After cutting the abdomen of the sacrificed experimental animals, blood was collected from the vena cava and centrifuged immediately at 3000 rpm for 20 minutes. Serum was separated through the centrifugation and used directly in the experiment. First, 10 μl of the serum of the isolated experimental animal was added to a 96-well microlplate, and 25 μl of the three kinds of Vipase fractions prepared in Example 1 were added, respectively, and then glycyl-prolyl- having a final concentration of 50 μM. 25 μl of 7-amino-4-methyl-coumarin (Gly-Pro-AMC) was added and reacted at 37 ° C. for 60 minutes. The vifaci fraction solution was prepared by adjusting the respective vifaci fraction in solvent to a final concentration of 250 μg / mL, 500 μg / mL and 1,000 μg / mL, respectively.
상기 반응 후에 반응 정지 시약인 3 M의 아세트산(acetic acid)을 70 μl씩 첨가하고, Multilabel counter(VICTOR3V 1420-040, PerkinElmer Inc, USA)를 이용하여 excitation 370 nm 및 emission 440 nm의 조건에서 형광도를 측정하였다. 상기 측정된 결과를 표 2에 나타내었다.After the reaction, 70 μl of 3 M acetic acid (acetic acid) as a reaction stopping reagent was added, and the fluorescence at the conditions of excitation 370 nm and emission 440 nm using a Multilabel counter (VICTOR3V 1420-040, PerkinElmer Inc, USA) Was measured. The measured results are shown in Table 2.
상기 표 2에 나타낸 바와 같이, 상기 분획용매 중, 헥산 분획용매로 제조된 분획물의 경우, 상기 Cell-free system에서의 시험관내 실험을 통하여 DPP-IV 활성 억제여부를 측정한 결과, 다른 분획용매인 에틸아세테이트나 물을 분획용매로 이용한 경우에 비하여 헥산분획의 억제효율이 동일 농도의 경우 약 350% 내지 400%의 억제효율을 갖고, 헥산 분획 500 μg/mL의 함량으로도 에틸아세테이트 분획 또는 물 분획 1,000 μg/mL을 처리의 약 200%에 해당하는 억제효율을 나타내는 것으로 확인되어, 상기 헥산 분획의 경우 다른 분획용매에 의해 제조된 분획에 비하여 현저하게 우수한 효과를 갖는 것으로 확인되었다.
As shown in Table 2, in the fractionated solvents, fractions prepared with the hexane fractionation solvent were measured for inhibition of DPP-IV activity through in vitro experiments in the cell-free system. The inhibitory efficiency of the hexane fraction was about 350% to 400% at the same concentration compared to the case where ethyl acetate or water was used as the fractionation solvent, and the ethyl acetate fraction or the water fraction even with the content of 500 μg / mL of the hexane fraction. It was confirmed that 1,000 μg / mL exhibited an inhibitory efficiency corresponding to about 200% of the treatment, and the hexane fraction was found to have a remarkably superior effect compared to the fraction prepared by other fraction solvents.
제조예Production Example : : 비파씨Mr. Biff 추출물을 이용한 제제 Formulation with Extract
제조예Production Example 1. One. 산제의Powder 제조 Produce
비파씨 추출물 분말 20 mg
유당 100 mg
탈크 10 mg
상기의 성분들을 혼합하고 기밀포에 충진하여 산제를 제조하였다.
The above ingredients were mixed and filled in an airtight cloth to prepare a powder.
제조예Production Example 2. 정제의 제조 2. Preparation of Tablets
비파씨 추출물 분말 10 mg
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg 2 mg magnesium stearate
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.
After mixing the above components was prepared by tableting according to the conventional manufacturing method of the tablet.
제조예Production Example 3. 캡슐제의 제조 3. Preparation of Capsule
비파씨 추출물 분말 10 mg
결정성 셀룰로오스 3 mg 3 mg of crystalline cellulose
락토오스 14.8 mg Lactose 14.8 mg
마그네슘 스테아레이트 0.2 mg Magnesium Stearate 0.2 mg
통상의 캡슐제 제조방법에 따라 상기의 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조하였다.
According to a conventional capsule preparation method, the above ingredients were mixed and filled into gelatin capsules to prepare capsules.
제조예Production Example 4. 4. 액제의Liquid 제조 Produce
비파씨 추출물 분말 20 mg
이성화당 10 g 10 g of isomerized sugar
만니톨 5 g 5 g of mannitol
정제수 적량 Purified water quantity
통상의 액제의 제조방법에 따라 정제수에 각각의 성분을 가하여 용해시키고 레몬향을 적량 가한 다음 상기의 성분을 혼합한 다음 정제수를 가하여 전체를 정제수를 가하여 전체 100㎖로 조절한 후 갈색병에 충진하여 멸균시켜 액제를 제조하였다.
After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution was prepared by sterilization.
제조예Production Example 5. 건강기능식품의 제조 5. Preparation of dietary supplements
비파씨 추출물 분말 1000 ㎎ Vifase Extract Powder 1000mg
비타민 혼합물 적량 Vitamin mixture quantity
비타민 A 아세테이트 70 ㎍ 70 μg of Vitamin A Acetate
비타민 E 1.0 ㎎ Vitamin E 1.0 mg
비타민 B1 0.13 ㎎ Vitamin B1 0.13 mg
비타민 B2 0.15 ㎎ Vitamin B2 0.15 mg
비타민 B6 0.5 ㎎ Vitamin B6 0.5 mg
비타민 B12 0.2 ㎍ 0.2 μg of vitamin B12
비타민 C 10 ㎎
비오틴 10 ㎍ 10 μg biotin
니코틴산아미드 1.7 ㎎ Nicotinic Acid 1.7 mg
엽산 50 ㎍ 50 μg folic acid
판토텐산 칼슘 0.5 ㎎ Calcium Pantothenate 0.5mg
무기질 혼합물 적량 Mineral mixture
황산제1철 1.75 ㎎ Ferrous Sulfate 1.75 mg
산화아연 0.82 ㎎ Zinc Oxide 0.82 mg
탄산마그네슘 25.3 ㎎ Magnesium carbonate 25.3 mg
제1인산칼륨 15 ㎎ Potassium monophosphate 15 mg
제2인산칼슘 55 ㎎
구연산칼륨 90 ㎎ Potassium Citrate 90 mg
탄산칼슘 100 ㎎
염화마그네슘 24.8 ㎎ Magnesium chloride 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 건강기능식품에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강기능식품 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강기능식품 제조에 사용할 수 있다.Although the composition ratio of the above-mentioned vitamin and mineral mixtures is a composition that is relatively suitable for the health functional food, the composition is mixed in a preferred embodiment, but the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. The granules can then be prepared and used in the manufacture of nutraceuticals in accordance with conventional methods.
Claims (3)
상기 용매 추출물은 탄소수 1 내지 4의 알코올 수용액을 추출용매로 추출한 것인 당뇨병 치료 또는 예방용 조성물. The method of claim 1,
The solvent extract is a composition for treating or preventing diabetes that is extracted from the aqueous solution of alcohol having 1 to 4 carbon atoms as an extraction solvent.
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KR20190103665A (en) | 2018-02-28 | 2019-09-05 | 목포대학교산학협력단 | Food composition for improving or preventing obesity containing extract of saururus chinensis |
KR102106152B1 (en) * | 2018-11-13 | 2020-05-04 | 신원수 | Manufacturing method of sambungnyawa jelly and the jellies thereby |
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KR100673068B1 (en) | 2005-04-07 | 2007-01-22 | 대구한의대학교산학협력단 | A composition comprising the leaf extract of Eriobotrya japonica for treating and preventing obesity disease |
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KR100673068B1 (en) | 2005-04-07 | 2007-01-22 | 대구한의대학교산학협력단 | A composition comprising the leaf extract of Eriobotrya japonica for treating and preventing obesity disease |
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KR20190103665A (en) | 2018-02-28 | 2019-09-05 | 목포대학교산학협력단 | Food composition for improving or preventing obesity containing extract of saururus chinensis |
KR102106152B1 (en) * | 2018-11-13 | 2020-05-04 | 신원수 | Manufacturing method of sambungnyawa jelly and the jellies thereby |
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