KR101026857B1 - Composition for preventing or improving the diabete containing eriobotrya japonica extract - Google Patents

Abstract

The present invention relates to a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient. In particular, the composition for the treatment or prevention of diabetes comprising the loquat extract according to the present invention as an active ingredient can control blood sugar, glycated hemoglobin and insulin, so it is not only excellent in the treatment and prevention of diabetes, but also natural substances as an active ingredient. It can be widely used to treat or prevent diabetes without causing problems of side effects.

Loquat, diabetes, blood sugar, insulin

Description

COMPOSITION FOR PREVENTING OR IMPROVING THE DIABETE CONTAINING ERIOBOTRYA JAPONICA EXTRACT}

The present invention relates to a composition for treating or preventing diabetes.

Diabetes refers to a disorder in which the balance between insulin sensitivity and insulin secretion in the body is broken, that is, insulin deficiency or insulin sensitivity is reduced and glucose is released into the urine because blood glucose is not controlled due to inability to use blood sugar.

The diabetes is largely divided into type 1 diabetes and type 2 diabetes. The type 1 diabetes mellitus, 'insulin dependent diabetes mellitus', is caused by a decrease in insulin production and secretion due to damage to the pancreatic beta cells (β-cells) that secrete insulin. In order to treat the type 1 diabetes, generally, insulin is externally administered to lower blood sugar. The type 2 diabetes, that is, 'insulin-independent diabetes' refers to a state in which blood sugar levels are higher than normal, and when the balance of insulin secretion and the secretion of insulin from the pancreas is broken in peripheral tissues such as muscle, fat, and liver. Occurs. Most diabetics have insulin resistance and hyperinsulinemia before progressing to type 2 diabetes. In other words, type 2 diabetes has a decrease in the action of insulin to lower the initial blood sugar, resulting in insulin resistance that does not effectively absorb sugar and utilize it as energy in peripheral tissues, resulting in insulin secretion to lower high blood sugar Increased hyperinsulinemia occurs. Failure to overcome this will eventually lead to type 2 diabetes. In Korea, the number of patients with type 2 diabetes has increased in recent decades.

The increase in diabetes prevalence is attributed to economic growth, changes in dietary and lifestyle habits, and increased ethnic and family history factors and obesity rates.

In diabetics, it is important to maintain normal blood sugar through diet or medication. However, in case of type 2 diabetes, glycemic control may not be easy even though non-pharmaceutical treatment is performed through diet and exercise. In this case, oral hypoglycemic agents or insulin are required. However, oral hypoglycemics, which are widely used, can cause hypoglycemia as a side effect of beta cell exhaustion, and side effects such as lactic acidosis may occur. In addition, insulin injection is inconvenient to use and there is a risk of hypoglycemia, long-term use of obesity can be promoted, it may cause difficulties in long-term blood sugar management. Therefore, there is an increasing demand for developing a naturally-derived antidiabetic material with less side effects and easy administration in type 2 diabetes patients.

The present invention is to solve the problems of the prior art as described above, it is an object of the present invention to provide a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient.

The present invention provides a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient in order to achieve the above object.

In addition, to achieve the above object, the present invention provides a food composition for improving or preventing diabetes comprising a loquat extract as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention, while studying a natural substance-derived antidiabetic material with less side effects and easy to administer, it was confirmed that loquat extract can lower the amount of blood sugar and glycated hemoglobin, based on which the present invention was completed.

In the present invention, loquat ( Eriobotrya japonica ) is a plant belonging to the rose family Evergreen arboreal, with thick branches and dense yellowish brown hairs along with the back of leaves. Leaves are alternate, upside-down, broad-shaped bar-shaped, with serrated teeth on the edges, and fruit is spherical or oval, used for food. Loquat is known to be effective in clearing, Jinhae, expectoration, septic breakdown, or edema in existing civilian use. The loquat may preferably be loquat seed or loquat leaf, more preferably loquat seed. In the case of loquat seed, when the loquat seed extract was administered for 3 weeks, blood sugar dropped to less than half of the control group, and the amount of glycated hemoglobin was significantly reduced in comparison with the loquat leaf extract as well as the control group. Compared with loquat leaf extract, it showed an effect of increasing insulin secretion about 2 times or more, and thus, it was confirmed that there was a remarkable effect for treating and preventing diabetes in terms of blood glucose and insulin secretion.

The extract of the present invention can be prepared by extracting with an extraction solvent or by adding a fractional solvent to the crude extract prepared by extraction with an extraction solvent.

The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as alcohol having 1 to 5 carbon atoms such as methanol or ethanol, ethyl acetate or acetone, and a nonpolar solvent of ether, chloroform, benzene, hexane or dichloromethane, or a mixed solvent thereof.

The extraction solvent of the present invention may be at least one solvent selected from the group consisting of water and alcohols having 1 to 4 carbon atoms, more preferably an aqueous solution of alcohols having 1 to 4 carbon atoms such as 50 to 99% ethanol, Even more preferably, it may be an aqueous solution of alcohol having 1 to 4 carbon atoms such as 70 to 95% ethanol.

The extract of the present invention may be prepared according to a conventional land plant, preferably a method of preparing an extract for extracting leaves or seeds, and specifically, may be cold extraction, hot extraction or thermal extraction, and the like. Extraction apparatus, ultrasonic grinding extractor or fractionator can be used.

In addition, the extract of the present invention may be a fraction further subjected to the fractionation process after adding a fractional solvent to the crude extract extracted with the solvent. The fractional solvent may be a solvent selected from the group consisting of ethyl acetate, ether, chloroform, benzene, hexane, methylene chloride and mixed solvents thereof, preferably ethyl acetate.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In one embodiment of the present invention, the loquat extract may be obtained by the following method.

Loquat leaves and loquat seeds were extracted with 80% methanol for 48 hours, and prepared by separating only the supernatant by depressurizing filtration. The prepared extract was concentrated under reduced pressure in a water bath of 50 and 24 in a freezer of -70. Freeze drying for time.

The present invention relates to a composition for treating or preventing diabetes comprising a loquat extract as an active ingredient.

In the present invention, diabetes is when the balance between insulin sensitivity and insulin secretion in the body is broken, that is, insulin deficiency or insulin sensitivity is not available because the blood sugar is not used to control the blood sugar to release glucose in the urine Means disease. The diabetes is largely divided into type 1 diabetes and type 2 diabetes, and the diabetes may be preferably type 2 diabetes.

Diabetic treatment or prevention composition comprising a loquat extract of the present invention as an active ingredient may comprise 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight of the loquat extract based on the total weight of the composition.

Diabetic treatment or prevention composition comprising the loquat extract as an active ingredient does not cause a problem of side effects in that it is an extract of natural substances, and has an excellent effect in that there is an improvement or prevention effect of diabetes.

Diabetic treatment or prevention composition comprising a loquat extract of the present invention as an active ingredient can be applied directly to humans.

Diabetic treatment or prevention composition comprising the loquat extract as an active ingredient may comprise the loquat extract alone as an active ingredient, other ingredients, pharmaceutically acceptable or nutritious according to the formulation, method and purpose of use. It may further comprise a pharmaceutically acceptable carrier, excipient, diluent or accessory.

More specifically, the composition for treating or preventing diabetes comprising the loquat extract as an active ingredient may further include nutrients, vitamins, electrolytes, flavors, coloring agents, neutralizing agents, pectic acid and salts thereof, alginic acid and salts thereof in addition to the active ingredient. , Organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the carrier, excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, acycia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline In the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, saline It may be one or more selected, but is not limited to any conventional carrier, excipient or diluent can be used. The ingredients may be added independently or in combination with the loquat extract, which is the active ingredient.

The composition for treating or preventing diabetes comprising the loquat extract as an active ingredient is 0.001 wt% to 99.9 wt%, preferably 0.1 wt% to 99 wt%, more preferably 1 wt% of the loquat extract, based on the total weight of the composition. % To 50% by weight. In addition, the content of the additional component is preferably added in the range of 0.1 to 20 parts by weight per 100 parts by weight of the composition for treating or preventing diabetes.

In addition, the composition for treating or preventing diabetes comprising the loquat extract as an active ingredient, when formulated, common fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, flavors, emulsifiers or preservatives, etc. It may further include, can be used both oral or parenteral.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, water, and the like in the loquat extract. It is prepared by mixing sucrose, lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may include, for example, wetting agents, sweeteners, fragrances, and preservatives. have.

In addition, the formulation of the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may be in a preferred form depending on the method of use, and in particular, provides rapid, sustained or delayed release of the active ingredient after administration to a mammal. It can be formulated by adopting a method known in the art to do so. Examples of specific formulations include granules, powders, syrups, solutions, suspensions, tablets, injections, spirits, cataplasma, capsules, soft or hard gelatin capsules, and the like.

Furthermore, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may be prepared using Remington's Pharmaceutical Sciences (Remington's Pharmaceutical Science, recent edition), Mack Publishing Company, Easton PA using appropriate methods known in the art. Formulated preferably using the method disclosed herein.

The dosage of the composition for treating or preventing diabetes comprising the loquat extract according to the present invention as an active ingredient may be appropriately selected by those skilled in the art in consideration of the method of administration, the age, sex and weight of the recipient, and the severity of the disease. have. For example, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient is 0.0001 mg / kg to 1000 mg / kg, based on the loquat extract, to 0.01 mg / kg to be more effective. To 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient, in addition to the loquat extract may further include an extract for a compound or natural product having a known diabetes treatment or prevention effect, the loquat extract 5 parts by weight to 200 parts by weight with respect to 100 parts by weight, respectively.

In addition, in order to achieve the above object, the present invention provides a food composition for preventing or improving diabetes comprising the loquat extract as an active ingredient.

In the present specification, the term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process. It is intended to include all dietary supplements, beverages, food additives and beverage additives.

Examples of the food of the present invention include various foods, beverages, gums, teas, vitamin complexes, and health functional foods. In addition, the food in the present invention includes special nutritional products (e.g., prepared oils, infants, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( For example, soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruits, vegetable drinks, soy milk, fermented beverages, and the like, and natural seasonings (eg, ramen soup). The food, health functional food, beverages, food additives and beverage additives may be prepared by conventional manufacturing methods.

In the present invention, the health functional food is a biological defense rhythm control, disease prevention and the like having a food group or a food composition that has added value to the food by using physical, biochemical, biotechnological techniques, etc. It means a food that is designed and processed to fully express the gymnastics function on recovery.

The dietary supplement may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of dietary supplements.

In the present invention, the drink refers to a generic term for drinking to quench thirst or to enjoy the taste and is intended to include a functional beverage. The beverage is not particularly limited to other ingredients other than including the loquat extract as an active ingredient in the indicated ratio as an active ingredient, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. .

Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate may generally be from about 1 to 20 g, preferably from 5 to 12 g per 100 food compositions of the invention The composition of the present invention is intended for the preparation of natural fruit juices, fruit juice drinks, vegetable drinks It may further contain a pulp for.

In addition to the above, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. These components can be used independently or in combination. The proportion of such additives is not so critical, but may be selected in the range of 0 to 2,000 parts by weight per 100 parts by weight of loquat extract of the present invention.

In the present invention, the health functional beverage is a biological defense rhythm control or disease prevention of a beverage group or beverage composition which has added value to the beverage by using physical, biochemical and biotechnological techniques to act and express the function of the beverage for a specific purpose. Means a beverage that is designed and processed to fully express the gymnastic function related to recovery and the like.

The health functional beverage is not particularly limited to other ingredients except for containing the loquat extract of the present invention as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks.

Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and xylitol Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1 to 20 g, preferably 5 to 12 g per 100 compositions of the invention.

In addition, in the diabetic prevention or improvement food composition comprising a loquat extract as an active ingredient, the amount of loquat extract may include 0.01 to 15% by weight of the total food weight, the beverage composition is 0.02 to 5 based on 100 g, preferably 0.3 to 1 g.

The loquat extract of the present invention is confirmed through the change in blood glucose, the amount of glycated hemoglobin, and the amount of insulin secreted, lowering the amount of glucose and glycated hemoglobin, and increasing the amount of insulin secreted to show the effect of treating or preventing diabetes including hypoglycemic effect It became.

As described above, ingestion of the composition for treating or preventing diabetes comprising the loquat extract of the present invention as an active ingredient may lower blood sugar while increasing the amount of glycated hemoglobin, and increase the amount of insulin secreted, thereby treating diabetes or Not only is it effective in preventing, and unlike the existing anti-diabetic drugs, which are artificial compounds, natural salt, natural salt does not have any side effects, and furthermore, it is economically economical because it uses specialized resources, abundantly produced in Korea, especially Jeollanam-do. Advantageously, the composition for treating or preventing diabetes containing loquat extract as an active ingredient will be very effective industrially.

Hereinafter, preferred embodiments of the present invention will be described. The following examples are only for illustrating the present invention and the scope of the present invention is not limited by the following examples.

[ Example ]

Example 1 Preparation of Loquat Extract

Loquat leaves obtained from Mokpo National University herbal medicine and loquat seeds collected from Wando were extracted with 80% methanol for 48 hours, and the supernatant was separated by vacuum filtration to prepare loquat leaf extract and loquat seed extract, respectively. The prepared extract was concentrated under reduced pressure in a water bath of 50, frozen in a freezer at -70 for 24 hours, and dried using a freeze dryer system to prepare a loquat extract sample.

Extracts prepared in this way were stored in freezer -20 until used in the experiment.

Example 2 Determination of Diabetic Therapeutic Effect of Loquat Extract

The anti-diabetic effect of the loquat extract prepared in Example 1 was measured using a laboratory mouse of type 2 diabetes animal model (C57BL / Ks db / db mice).

2-1. Breeding of Experimental Animals

Experimental animals were fed 6 weeks-old male db / db obese mice (C57BL / Ks db / db mice) from Harlan Co., Ltd .. Adapted to 2018S Teklad Global 18% Protein Rodent Diet (Harlan) from 3 days before the experiment, 8 rats per group based on body weight, Control, E. japonica seeds, and Loquat leaf extract ( E. Japonica leaves) were divided into three groups and fed into three groups. The dosage of the extract was 200 mg / kg at the same time every day (10 am) for 6 weeks. The diet and water were supplied fresh every day, and the animal breeding room maintained the temperature, humidity and light for 6 weeks. Breeding.

2-2. Dietary Intake and Water Intake Measurement

The dietary intake and the water intake of the experimental animals of Example 2-1 were measured once a week by subtracting the remaining amount from the feed the day before, and the results are shown in FIGS. 1 and 2.

1 and 2, the dietary intake and water intake showed a tendency to decrease in the experimental group ingested loquat extract compared to the control group during the experimental period, especially intake of the loquat seed extract compared to the control group Both intake and water intake were significantly decreased.

In general, type 2 diabetes is not as severe as type 1 diabetes, but the cells do not use enough sugar to cause polymorphism, and to show the symptoms of polyuria to excrete excess blood sugar, causing thirst. Dehydration may occur due to lack of moisture in the stomach. Considering the symptoms of diabetes, it was expected that the loquat seed extract, which had a significantly reduced dietary intake and water intake, was effective in treating or preventing diabetes.

2-3. Measurement of blood sugar, glycated hemoglobin (HbA1c) and insulin levels

After the animal was bred for 12 hours before sacrifice, blood was collected using a heparin-treated syringe from the heart, and some of them were measured for blood sugar and glycated hemoglobin, and the remaining blood was centrifuged at 3000 rpm for 15 minutes for serum. After obtaining the cryopreservation, the serum insulin (insulin) amount was analyzed from the serum.

Blood glucose was measured from the collected blood three weeks after oral administration of the extract and two times at sacrifice, at three weeks after oral administration, at the tail vein, and at the last sacrifice, from the blood of the heart. (Medisens Optium). In addition, glycated hemoglobin was measured from the whole blood collected from the heart after sacrifice using an in2it (I) analyzer (Bio-Rad). In addition, FER (Food Efficiency Ratio) represents the dietary efficiency, calculated as the weight gain compared to the intake (g). In addition, the amount of insulin was measured using the insulin ELISA kit (Shibayagi) for mice from the separated serum.

The measured results are shown in Table 1 below. All the values were repeated at least two times, and the mean ± standard error was compared with each group. After the ANOVA test, post-test was performed using Duncan's multiple comparison (p <0.05).

TABLE 1

division FER Blood glucose (mg / dl) HbA1c insulin
(insulin) 3rd week Final (%) (ng / ml) Control
(Control) 0.031 ± 0.005 330.5 ± 23.42 391.87 ± 24.79 11.87 ± 0.27 5.26 ± 0.48 Loquat Seed Extract
( E. japonica seeds) 0.042 ± 0.003 159.1 ± 13.58 375.14 ± 45.01 9.76 ± 0.36 8.30 ± 0.72 Loquat Leaf Extract
( E. japonica leaves) 0.02 ± 0.003 286.75 ± 18.26 414.28 ± 17.08 10.81 ± 0.41 6.60 ± 0.61

As shown in Table 1, fasting blood glucose was decreased compared to the control group after 3 weeks after the administration of the extract and at the end of the experiment. In particular, in the case of vipa seed extract 3 weeks after the administration of the extract blood sugar was confirmed to be reduced to about half of the blood sugar of the control group, it was confirmed that significantly reduced compared to the control group. In addition, unlike the blood glucose level, which is affected by short-term environmental changes, the glycated hemoglobin, which is a standard that can change the blood glucose level, is compared with the control group, and both the group treated with the vifa seed extract and the group with the loquat leaf extract decreased the glycated hemoglobin level. In particular, the loquat seed administration group showed a reduced amount of about 2 times compared to the control group compared to the loquat leaf administration group, it was confirmed that there is a remarkably excellent glycated hemoglobin reduction effect, that is significantly excellent glycemic control effect. In addition, in the case of insulin amount, the administration of loquat extract was confirmed to increase the amount of insulin compared to the control group, it was expected to be able to compensate the insulin resistance of type 2 diabetes. Particularly, in the case of the vipa seed extract, compared to the bifa leaf extract, the increase in insulin was found to be about 2.5 times based on the amount of insulin in the control group, thereby remarkably excellent diabetes treatment by compensating for type 2 diabetes and related insulin resistance. It was also expected to have a preventive effect.

Production Example : Formulation using loquat extract

Production Example  One. Powder  Produce

Loquat Extract Powder 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

Production Example  2. Preparation of Tablets

Loquat Extract Powder 10 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components was prepared by tableting according to the conventional manufacturing method of the tablet.

Production Example  3. Preparation of Capsule

Loquat Extract Powder 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients were mixed and filled into gelatin capsules to prepare capsules.

Production Example  4. Liquid  Produce

Loquat Extract Powder 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water quantity

According to the conventional method of preparing a liquid solution, each component is added to the purified water to dissolve, the lemon flavor is appropriately added, the above components are mixed, the purified water is added, the whole is adjusted to 100 by adding purified water, and then filled into a brown bottle and sterilized. To prepare a liquid formulation.

Production Example  5. Preparation of dietary supplements

Loquat Extract Powder 1000

Vitamin mixture quantity

Vitamin A Acetate 70

Vitamin E 1.0

Vitamin B1 0.13

Vitamin B2 0.15

Vitamin B6 0.5

Vitamin B12 0.2

Vitamin C 10

Biotin 10

Nicotinamide 1.7

Folic acid 50

Calcium Pantothenate 0.5

Mineral mixture

Ferrous Sulfate 1.75

Zinc Oxide 0.82

Magnesium Carbonate 25.3

Potassium Phosphate Monobasic 15

Dicalcium Phosphate 55

Potassium Citrate 90

Calcium Carbonate 100

Magnesium chloride 24.8

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a composition suitable for a health functional food in a preferred embodiment, the compounding ratio may be arbitrarily modified. The granules can then be prepared and used in the manufacture of nutraceuticals in accordance with conventional methods.

1 is a graph showing a change in the intake of the diet during the breeding period of the experimental animal according to an embodiment of the present invention.

2 is a graph showing a change in water intake during the breeding period of the experimental animal according to an embodiment of the present invention.

Claims (5)

delete delete Loquat ( Eriobotrya japonica ) A composition for treating or preventing diabetes comprising the extract as an active ingredient. Bipa ( Eriobotrya japonica ) food composition for preventing or improving diabetes comprising an extract as an active ingredient. delete

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