KR20200101114A - Food composition comprising Chrysanthemum zawadskii var extract - Google Patents

Abstract

The present invention relates to a health functional food for alleviating fatigue. The health functional food for improving fatigue of the present invention contains a mixed extract mixing flowers, stems, and roots of chrysanthemum zawadskii var as an active ingredient, thereby reducing and alleviating accumulation of the fatigue. In addition, the health functional food for alleviating fatigue are non-toxic and can be consumed in a form of food.

Description

Health functional food for improving fatigue containing Gujeolcho extract as an active ingredient {Food composition comprising Chrysanthemum zawadskii var extract}

The present invention relates to a health functional food containing an extract of Gujeolcho that can improve fatigue as an active ingredient.

Fatigue can be divided into physical fatigue and mental fatigue, and in the normal case, it is easily eliminated by 7 to 9 hours of rest, but when fatigue is excessively accumulated, it is not easily resolved by sleep or rest alone.

Physical fatigue, which is caused irregularly and excessively for a long period of time, leads to deterioration of the performance of the muscular and skeletal system, and various symptoms such as muscle pain, body aches, and sleepiness may occur, and may cause chronic fatigue syndrome accompanied by mental fatigue symptoms.

Modern people in a busy life are easily fatigued due to lack of activity, mental stress, lazy habits, and obesity, and the interest and desire to maintain health without disease is increasing.

In general, physical fatigue is caused by excessive exercise or physical labor, which causes damage to muscle cells due to factors such as increase of reactive oxygen species, accumulation of lipid peroxides, etc. from oxidative stress caused by excessive energy metabolism, and thus lactic acid is produced. It causes fatigue due to formation and accumulation. Therefore, supplementation of energy sources that can protect against oxidative stress and reduction of accumulation of fatigue substances can be regarded as typical fatigue improvement effects.

Accordingly, modern people suffering from chronic fatigue prefer to consume health functional foods to supplement nutrients for fatigue recovery, health promotion, and disease prevention.

On the other hand, Gujeolcho has only known effects such as anti-diabetes, and the effects related to fatigue improvement are not known. Accordingly, in the present invention, the extract of Gujeolcho was treated on mouse-derived myoblasts to induce oxidative stress and then the protective effect of muscle cells was confirmed. Based on this, the present invention intends to provide a health functional food for improving fatigue using Gujeolcho extract.

Korean Registered Patent No. 1888756 Republic of Korea Patent Publication No. 2018-0052514

An object of the present invention is to provide a health functional food containing a mixed extract of Gujeolcho flowers, stems, and roots that can improve fatigue as an active ingredient.

Health functional food that can improve the fatigue of the present invention to achieve the above object may contain a mixed extract of Gujeolcho flower, stem and root mixed as an active ingredient.

The Gujeolcho mixed extract may be obtained by mixing the flowers, stems, and roots of Gujeolcho in a weight ratio of 1: 1-3: 0.5-1.

Health functional food containing the Gujeolcho mixed extract of the present invention as an active ingredient is excellent in fatigue improvement effect.

In particular, Gujeolcho is a natural medicinal plant and can be edible, so the composition of the present invention comprising an extract derived therefrom as an active ingredient has a safe advantage even for long-term use.

1 is a graph showing the fluorescence intensity of reactive oxygen species in myoblasts when using the Gujeolcho mixed extract of the present invention.

The present invention relates to a health functional food containing Gujeolcho extract as an active ingredient to improve fatigue.

Hereinafter, the present invention will be described in detail.

The health functional food capable of improving fatigue of the present invention contains a mixed extract of Gujeolcho flowers, stems and roots as an active ingredient.

Gujeolcho ( Chrysanthemum zawadskii var.latilobum (Maxim.) Kitam.) used in the present invention is a perennial herb of the chrysanthemum family, and is also referred to as yaguk (野菊), sunmocho (仙母草), gobong (苦蓬), wild chrysanthemum. It grows wildly in mountainous and highlands nationwide, and grows in China, Russia, Mongolia, and Japan, and has been used in the form of a pill or decoction in the form of a pill after collecting stems and leaves in the private sector in the fall. It reproduces while the rootstock in the ground extends sideways, and the flowering period is August-October and the fruiting period is October-November. The herbal names of Gujeolcho outpost are called Seonmocho, Gujeolcho, and the flowers are elegant and beautiful, so they are widely cultivated for landscape creation. Gujeolcho outpost and flower spikes have been used for pneumonia, bronchitis, cough, cold, women's disease, poor circulation, gastrointestinal disease and high blood pressure.

Gujeolcho extract according to the present invention is a mixed extract of Gujeolcho flowers, stems and roots mixed, the Gujeolcho mixed extract is the flowers, stems and roots of Gujeolcho in a weight ratio of 1: 1-3: 0.5-1, preferably 1 : 1-2: extracted by mixing at a weight ratio of 0.6-0.8. If the content of the stem and root based on the flower of Gujeolcho is outside the above range, the effect of improving fatigue may be poor.

When the Gujeolcho mixed extract of the present invention is not used, and a Gujeolcho flower extract, a Gujeolcho stem extract, or a Gujeolcho root extract is used, the fatigue improvement effect may be significantly reduced compared to the Gujeolcho mixed extract. In particular, Gujeolcho flower extract hardly causes fatigue improvement effect.

The extract of the present invention is an extract extracted for 5 to 10 hours, preferably 7 to 8 hours at 60 to 90 ℃. When the extraction temperature and time are less than the lower limit, the active ingredient may not be extracted, and when the extraction temperature and time are greater than the upper limit, the burnt taste is strong and the nutrient component may be destroyed.

The extraction solvent is not particularly limited, but may include water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. Since the extract extracted with water is superior to the extract extracted with an organic solvent, water It is preferable to use as an extraction solvent.

The Gujeolcho mixed extract prepared as described above may be used as an extract itself or may be powdered for convenience. The method of powdering the Gujeolcho mixed extract is to reduce the volume by concentrating the extract under reduced pressure, and lyophilize it with a freeze dryer to powder it.

The Gujeolcho mixed extract of the present invention also includes a Gujeolcho mixed product formulated so that a mixture of Gujeolcho flowers, stems and roots can be administered to an animal in a broad sense, such as a mixed powder of Gujeolcho flowers, stems and roots. Has meaning. Although the experiment was conducted with a mixed extract of Gujeolcho flowers, stems and roots in the present invention, it is expected by those skilled in the art that the desired effect can be achieved even in the same form as a mixed processed product of Gujeolcho flowers, stems and roots. It will be possible.

Meanwhile, in the present specification, the term "containing as an active ingredient" means including an amount sufficient to achieve the efficacy or activity of the mixed extract obtained by mixing Gujeolcho flowers, stems and roots. For example, the mixture of Gujeolcho flowers, stems, and roots is used in a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. The mixture of Gujeolcho flowers, stems, and roots is a natural product and does not have side effects on the human body even when excessive administration is applied. Therefore, the upper limit of the quantity of the mixture of Gujeolcho flowers, stems and roots included in the composition of the present invention is selected within an appropriate range It can be done.

The present invention provides a food composition that can improve fatigue by containing a mixed extract of Gujeolcho flowers, stems and roots as an active ingredient.

The food composition according to the present invention can be used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gum, ice cream, vitamin complexes, health supplements. Etc.

The food composition of the present invention may include not only a mixture containing a mixed extract of Gujeolcho flowers, stems and roots as an active ingredient, but also ingredients commonly added during food production, for example, proteins, carbohydrates, Includes fats, nutrients, flavoring and flavoring agents. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made of drinks and beverages, in addition to the mixture containing the Gujeolcho flower extract or Gujeolcho stem extract of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts And the like may additionally be included.

The present invention provides a health functional food comprising a food composition for improving fatigue comprising a mixed extract of Gujeolcho flowers, stems and roots as an active ingredient. Health functional foods are foods prepared by adding a mixture containing a mixed extract of Gujeolcho flowers, stems and roots to food materials such as beverages, teas, spices, gums, confectionery, etc., or by encapsulation, powdering, suspension, etc. However, when ingested, it means that it has a specific effect on health, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be consumed on a daily basis. The amount of the mixed extract of Gujeolcho flower, stem, and root mixed in such health functional foods cannot be uniformly regulated depending on the type of health functional food to be applied, but is added within the range that does not damage the original taste of the food. It is sufficient, and is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight, based on the target food. In addition, in the case of health functional foods in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides the use of a mixed extract of Gujeolcho flowers, stems and roots for the manufacture of food for improving fatigue. As described above, the mixed extract of Gujeolcho flowers, stems and roots may be used for fatigue improvement.

In addition, the present invention provides a method for improving fatigue comprising administering a mixed extract of Gujeolcho flowers, stems and roots in an effective amount to a mammal.

The term "mammal" as used herein refers to a mammal that is the subject of treatment, observation or experimentation, and preferably refers to a human.

Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention, but the following examples are only illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It is natural that such modifications and modifications fall within the appended claims.

Example 1. Gujeolcho mixed extract

Gujeolcho flowers, stems, and roots were washed, freeze-dried, and pulverized to obtain respective powders. Gujeolcho flower powder, Gujeolcho stem powder, and Gujeolcho root powder were mixed in a weight ratio of 1:1.2:0.7. 100 g of the mixed powder and 900 ml of purified water were mixed, extracted for 8 hours in a bath at 80° C., filtered under reduced pressure, concentrated, and freeze-dried to obtain a mixed extract powder of Gujeolcho.

Comparative Example 1. Gujeolcho flower extract

It was carried out in the same manner as in Example 1, but instead of a mixture of Gujeolcho flowers, stems and roots, only Gujeolcho flowers were used to obtain Gujeolcho flower extract powder.

Comparative Example 2. Gujeolcho stem extract

It was carried out in the same manner as in Example 1, but instead of a mixture of Gujeolcho flowers, stems and roots, only Gujeolcho stems were used to obtain Gujeolcho stem extract powder.

Comparative Example 3. Gujeolcho root extract

It was carried out in the same manner as in Example 1, but instead of a mixture of Gujeolcho flowers, stems, and roots, only Gujeolcho roots were used to obtain Gujeolcho root extract powder.

Comparative Example 4. Content

In the same manner as in Example 1, Gujeolcho flowers, stems, and roots were mixed in a weight ratio of 1:0.5:3 to obtain Gujeolcho mixed extract powder.

<Test Example>

Test Example 1. Yield measurement

The yield for the Gujeolcho mixed extract prepared according to Example 1 was calculated according to the following [Equation 1].

[Equation 1]

Yield (%) = (Weight of freeze-dried powder (g) / Weight of sample (g)) X 100

division Weight of sample (g) Weight of lyophilized powder (g) yield(%) Example 1 100 12.03 12.03 Comparative Example 1 100 9.15 9.15 Comparative Example 2 100 10.49 10.49 Comparative Example 3 100 13.15 13.15 Comparative Example 4 100 11.89 11.89

As shown in Table 1 above, it was confirmed that the yield of the Gujeolcho mixed extract prepared according to Example 1 of the present invention was about 12%.

Test Example 2. Cytotoxicity evaluation

The cytotoxicity of the Gujeolcho extract prepared in Examples and Comparative Examples on mouse-derived myoblasts (C2C12 cell line) was confirmed.

The cytotoxicity of Gujeolcho extract was analyzed using MTT{3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide}assay. Mouse-derived myoblasts were cultured in DMEM medium containing 10% FBS (Fetal bovine serum, fetal bovine serum) at 37°C, and then cultured cells were cultured in a 96-well plate using DMEM medium containing 10% FBS. After inoculation at a concentration of 1×10⁴ cell/ml and cultured for 24 hours, the Gujeolcho mixed extract prepared in Example 1 was treated in DMEM medium at 1000 μg/mL in each well and cultured for 24 hours. Thereafter, 10 μL of MTT reagent at a concentration of 5 mg/ml was treated in each well, and after 3 hours, the culture solution was removed, and 100 μL of dimethyl sulfoxide (DMSO) was dispensed into each well, and the absorbance was measured at 595 nm.

[Equation 2]

Cell viability (%) = (A/B)X100

A: absorbance of hot water extract treatment group

B: absorbance of the control group

division Concentration (mg/ml) 0 0.01 0.05 0.1 0.5 One Example 1 100 99.9 99.9 99.8 96.8 97.8 Comparative Example 1 100 98.9 98.6 97.1 97.4 96.9 Comparative Example 2 100 98.6 98.1 98.3 97.6 97.0 Comparative Example 3 100 97.5 98.0 97.7 97.1 96.1 Comparative Example 4 100 98.6 99.0 98.1 97.5 96.8

As shown in Table 2 above, the Gujeolcho mixed extract prepared according to Example 1 of the present invention and the extracts of Comparative Examples 1 to 4 did not show cytotoxicity according to all concentrations of 0 to 1000 μg/mL in C2C12 myoblasts. Confirmed.

Therefore, future experiments were performed using a high concentration of 1000 μg/mL.

Test Example 3. Cell protection effect confirmation from oxidative stress

In order to confirm the protective effect of the Gujeolcho extract prepared in Examples and Comparative Examples for protecting mouse-derived myoblasts (C2C12 cell line) from oxidative stress, the antioxidant stress inhibitory ability of Gujeolcho extracts of Examples and Comparative Examples was measured.

To protect muscle cells from oxidative stress, cultured cells were inoculated at a concentration of 1X10⁴ cells/ml using DMEM medium added with 10% FBS in a 96-well plate and incubated for 24 hours, followed by examples in each well. The Gujeolcho mixed extract prepared in 1 was treated in DMEM medium at 1000 μg/mL for 1 hour before, and H ₂ O ₂ 500 μM was treated and reacted for 24 hours to induce oxidative stress. Thereafter, 10 μL of MTT reagent at a concentration of 5 mg/ml was treated in each well, and after 3 hours, the culture solution was removed, and 100 μL of dimethyl sulfoxide (DMSO) was dispensed into each well, and the absorbance was measured at 595 nm.

Table 3 below is a measure of the cell survival rate of muscle cells from oxidative stress in myoblasts, and Table 4 below shows the effect of protecting muscle cells from oxidative stress in myoblasts of Gujeolcho extracts of Examples and Comparative Examples.

division Control H ₂ O ₂ treatment concentration (mM) One 0.5 0.25 Cell viability (%) 100 40.1 63.1 82.5

Classification (cell viability, %) Control H ₂ O ₂ 0.5 mM treatment 0 μg/ml 1000 μg/ml Example 1 100 63.1 75.8 Comparative Example 1 100 63.1 63.4 Comparative Example 2 100 63.1 63.7 Comparative Example 3 100 63.1 63.1 Comparative Example 4 100 63.1 66.8

As shown in Tables 3 and 4 above, H ₂ O ₂ showed low cell survival rate by inducing oxidative stress on muscle cells, and Gujeolcho mixed extract prepared according to Example 1 (1000 μg/mL) treated group The cell viability of was 75.8%, which showed a significant protective effect of about 19% against H ₂ O ₂ induced cell damage.

On the other hand, it was confirmed that the extracts prepared according to Comparative Examples 1 to 4 did not exhibit a protective effect against cell damage induced by H ₂ O ₂ .

That is, it was confirmed that the mixed extract of Gujeolcho of Example 1 had a protective effect on muscle cells from oxidative stress induced by H ₂ O ₂ .

Test Example 4. Analysis of active oxygen content in cells

The formation of harmful reactive oxygen species in the body is naturally produced in the process of using oxygen. Oxygen can become a powerful oxidizing agent because it is easy to acquire electrons. The degree of occurrence of these reactive oxygen species (ROS) was measured using the DCFH-DA (Dichlorofluorescin diacetate) method.

The ROS removal effect resulting from oxidative stress was determined by inoculating cultured muscle cells in a 96-well black plate at a concentration of 1X10⁴ cells/ml using DMEM medium with 10% FBS and incubating for 24 hours, and then in each well. The Gujeolcho mixed extract prepared in Example 1 was treated in DMEM medium at 1000 ug/mL for 1 hour before, and H ₂ O ₂ 500 uM was treated and reacted for 2 hours to induce oxidative stress. Thereafter, a DCFH-DA reagent at a concentration of 10 uM/ml was treated with 20 uL in each well, and after 30 minutes, fluorescence was measured under the conditions of λ excitation 485 nm and λ emission 535 nm using a fluorometer.

1 is a graph showing the fluorescence intensity of reactive oxygen species in myoblasts when using the Gujeolcho mixed extract of the present invention.

Table 5 below shows the area for free radicals in myoblasts when using the extracts of Examples and Comparative Examples.

Classification (ROSX10 ⁶ ) Control H ₂ O ₂ 0.5 mM treatment 0 μg/ml 1000 μg/ml Example 1 7.5 42.8 24.9 Comparative Example 1 7.5 42.8 41.7 Comparative Example 2 7.5 42.8 41.8 Comparative Example 3 7.5 42.8 42.3 Comparative Example 4 7.5 42.8 38.5

1 and Table 5 above, H ₂ O ₂ induced oxidative stress in muscle cells, and Gujeolcho extract was omitted (H ₂ O ₂ process; 0+H ₂ O ₂ ) significantly increased the amount of intracellular ROS generation compared to the control group, but in the group treated with Gujeolcho mixed extract (1000 μg/mL) prepared according to Example 1, in the group omitting Gujeolcho extract. In comparison, it was confirmed that the amount of ROS was significantly decreased.

On the other hand, it was confirmed that the extracts of Comparative Examples 1 to 4 did not decrease the amount of ROS incidence compared to the group omitting the Gujeolcho extract.

That is, it was confirmed that the mixed extract of Gujeolcho of Example 1 significantly reduced the amount of ROS generated from oxidative stress induced by H ₂ O ₂ , thereby having a protective effect on muscle cells.

Test Example 5. Antioxidant component analysis

5-1. Total polyphenol content (mgGA/g): The total polyphenol content of the Gujeolcho mixed extract prepared in Example 1 was measured using the Folin-Denis method. A test solution was prepared by adding distilled water to 1 mg of sample to make 1 mL. To 0.5 mL of test solution (1 mg/mL), 1 mL of 2% Na ₂ CO ₃ solution and 50 μL of Folin-Ciocalteu's reagent were sequentially added, and the absorbance was measured at 720 nm with a spectrophotometer, and the measured absorbance was Gallic acid equivalent. Converted from the standard curve and expressed as a content

5-2. Total flavonoid content (mg Rutin/g): The total flavonoid content of the Gujeolcho mixed extract prepared in Example 1 was measured. A test solution was prepared by adding distilled water to 1 mg of sample to make 1 mL. After adding 75 μL of 5% NaNO ₂ solution to 1 mL of test solution (1 mg/mL), reacting at room temperature for 5 minutes, 30 μL of 10% AlCl ₃ solution and 200 μL of 1M NaOH were added, and then at 510 nm with a spectrophotometer. After measuring the absorbance, the measured absorbance was converted from the Rutin equivalent Standard curve and expressed as a content.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Content of polyphenol (mgGA/g sample) 45.36 31.15 30.89 29.02 35.94 Flavonoid content (mg Rutin/g sample) 38.18 22.12 22.45 20.18 26.11

As shown in Table 6 above, the Gujeolcho mixed extract (1 mg/mL) prepared according to Example 1 of the present invention exhibited a polyphenol content of 45.36 mg GA/g compared to the standard gallic acid, compared to the standard substance rutin. It was confirmed that the flavonoid content of 38.18 mg Rutin/g was shown.

On the other hand, it was confirmed that the extracts of Comparative Examples 1 to 4 had lower polyphenol content and flavonoid content than the extract of Example 1.

Test Example 6. Antioxidant activity confirmation

6-1. ABTS radical scavenging activity analysis: ABTS {2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)} radical scavenging activity was analyzed for the extracts of Examples and Comparative Examples to confirm the antioxidant activity of Gujeolcho extract. . ABTS radical scavenging ability was measured using a cation decolorization assay.

After dissolving 1 mM AAPH{2,2'-azobis(2-amidinopropane)dihydrochloride} and 2.5 mM ABTS in 1,000 mL of PBS for 30 minutes in a constant temperature bath at 70° C. to form radicals, absorbance at 734 nm It was measured and diluted with PBS so that the absorbance value became 0.65. To 0.98 mL of the diluted ABTS radical solution, 0.02 mL (1 mg/mL) of the test solution prepared to 1 mL by adding distilled water to 1 mg of the sample was added and reacted for 20 minutes, and the change in absorbance was measured. The measured absorbance was converted from a standard curve using vitamin C and expressed as VCEAC (Vitamin C Equivalent Antioxidant Capacity).

6-2. DPPH radical scavenging ability analysis: In order to confirm the antioxidant effect of Gujeolcho extract, DPPH (α,α-diphenyl-β-picrylhydrazyl) radical scavenging ability was analyzed for the extracts of Examples and Comparative Examples. DPPH radical scavenging activity was measured by modifying the DPPH free radical assay.

To 1.45 mL of a 0.05 mM DPPH solution, 50 μL (1 mg/mL) of the test solution prepared to 1 mL by adding distilled water to a 1 mg sample was added and reacted for 30 minutes in a dark room, and the change in absorbance was changed at 517 nm. Measured. The measured absorbance was converted from the standard curve using vitamin C and expressed as VCEAC.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 ABTS radical scavenging activity (mg VCEAC/g) 30.47 11.58 12.15 10.48 14.11 DPPH radical scavenging activity (mg VCEAC/g) 24.94 9.48 10.09 8.67 13.41

As shown in Table 7 above, the Gujeolcho mixed extract (1 mg/mL) prepared according to Example 1 of the present invention has a free radical scavenging activity of 30.47 mg VCEAC/g when compared with the antioxidant activity of the standard vitamin C. As compared with the antioxidant activity of vitamin C, which is a standard substance, it was confirmed that the free radical scavenging activity of 24.94 mg VCEAC/g was shown.

On the other hand, it was confirmed that the extracts of Comparative Examples 1 to 4 had lower antioxidant activity compared to the extract of Example 1.

From the above results, it was confirmed that the mixed extract of Gujeolcho prepared according to Example 1 has excellent antioxidant activity, and also has an effect of protecting muscle cells from oxidative stress.

Hereinafter, examples of the formulation of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit it, but is intended to be described in detail.

Formulation Example 1. Preparation of functional beverage

1,000 mg of mixed extract powder obtained in Example 1

1,000 mg citric acid

100 g oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 mL with purified water

After mixing the above ingredients according to a normal health drink manufacturing method, stirring and heating the resulting solution at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and stored in a refrigerator. It is used to prepare the functional beverage composition of the invention.

The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences, such as the demand class, the country of demand, and the purpose of use.

Claims (2)

A health functional food for improving fatigue, characterized in that it contains a mixed extract of Gujeolcho flowers, stems and roots as an active ingredient. The health functional food for improving fatigue according to claim 1, wherein the Gujeolcho mixed extract is a mixture of flowers, stems and roots of Gujeolcho in a weight ratio of 1: 1-3: 0.5-1.

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