KR102453914B1 - Composition comprising the crude drugs complex for decreation or treating musculoskeletal disorders, Phamacopuncture injection of oriental medicinie containing the same, Injection containing the same - Google Patents

Abstract

The present invention relates to a composition for alleviating and/or treating symptoms of musculoskeletal disorders, and more specifically, a herbal composite composition capable of alleviating and/or treating symptoms of musculoskeletal disorders using natural herbal ingredients using a complex of several herbal ingredients, such as pale pink. to a novel herbal composite composition that can be provided in the form of oriental medicinal herbs or injections.

Description

Composition comprising the crude drugs complex for decreation or treating musculoskeletal disorders, Pharmacopuncture injection of oriental medicinie containing the same, Injection containing the same}

The present invention relates to a herbal composition for alleviating or treating musculoskeletal diseases using a herbal composite component, and is applied to acupuncture for oriental medicine and used as a medicinal acupuncture component of medicinal acupuncture, or by direct injection to alleviate, improve and/or It relates to a novel herbal composition that can be treated.

Musculoskeletal disorders are health disorders caused by factors such as repetitive motion, improper work posture, use of excessive force, physical contact with sharp surfaces, sports injuries, vibration and temperature. , muscles, bones, tendons, intervertebral discs, and surrounding body tissues, which cause pain or decrease in function. Associated Musculoskeletal Disorders (MSD), Cumulative Trauma Disorders (CTDs), Myofascial Pain Syndrome, Myofascial Pain Syndrome (MPS), Cervical postural syndrome (Tension neck syndrome), traumatic inflammation (lateral epicondylitis, e.g. Tennis elbow), medial epicondylitis (e.g. golfer's elbow), subacromial bursitis, bicipital tenosynovitis, supraspinatus tendonitis, ganglion ), lateral lordosis, kyphosis, lumbar sprain, ankle sprain, etc.

According to a survey by the HIRA, in 2019, one in three Koreans visited a hospital for musculoskeletal problems. and lowering productivity.

The clinical characteristics of musculoskeletal disorders include pain, aching, stiffness, numbness, and movement disorders due to pulling. And, there are painkillers, steroids, procedures, surgery, extracorporeal shock wave, acupuncture, moxibustion, cupping, decoction, and chuna therapy for the treatment of diseases of the musculoskeletal system. The reality is that the needs of patients are still not being met.

Republic of Korea Patent Publication No. 10-2020-0087452 (2020.07.21) Republic of Korea Patent Publication No. 10-2020-0055845 (2020.05.21)

As a result of long-term research on methods of alleviating, improving, and treating musculoskeletal disorders, the present inventors have taken a step further than the existing treatment methods, so that the safety that can better solve problems such as blood clots, blood clots, and inflammation, which are the causes of pain By developing various complex compositions with proven natural herbal medicines, it was confirmed that the fundamental improvement was achieved by direct injection into the site of musculoskeletal disorders with the herbal immersion method of natural herbal ingredients rather than the method of taking medicines such as painkillers. That is, the present invention found the optimal composition of natural herbal ingredients effective for alleviating and/or treating musculoskeletal disorders, and found an effective method for applying them to the treatment of musculoskeletal disorders, thereby completing the present invention, and the present invention is a natural herbal complex extract An object of the present invention is to provide a herbal composite composition for improving and treating musculoskeletal diseases using

In order to achieve the above object, the herbal composite composition for alleviation, improvement and/or treatment of musculoskeletal disorders of the present invention contains the essence obtained by distilling the extract obtained by extracting the herbal complex containing the light yellow color with an extraction solvent.

In addition, another object of the present invention is to provide a method for preparing a herbal composite composition for alleviating and/or treating the musculoskeletal disease, the first step of preparing a herbal composite including a pale yellow color; a second step of preparing a mixed solution by adding the herbal compound to an extraction solvent and stirring; Step 3 of heating and distilling the mixed solution to obtain a concentrated extract; and 4 steps of filtering the concentrated extract to obtain a concentrate; a process including a can be performed.

Another object of the present invention is to provide a herbal medicinal acupuncture solution comprising the herbal composite composition for alleviation and/or treatment of musculoskeletal disorders described above and a medicinal acupuncture method using the same.

In addition, another object of the present invention is to provide an injection containing the herbal composite composition for alleviating and / or treating musculoskeletal disorders described above.

The herbal composition for alleviation, improvement and/or treatment of musculoskeletal diseases of the present invention is directly injected into the affected area through acupuncture, injection, etc., unlike existing therapeutic agents that have been absorbed and acted upon by the digestive system by drinking, so the action of the drug is delivered only to the musculoskeletal system As a targeted treatment that enables oriental medicinal acupuncture or injection, it is applied to acupuncture points that are effective for musculoskeletal disorders. By improving blood clots, blood clots, and inflammation in musculoskeletal disorders, pain, aching, stiffness, numbness, and tightness are gradually reduced, and the affected area is restored to normal Qi and blood circulation, so pain is quickly improved and further effective treatment is possible.

Hereinafter, the present invention will be described in more detail.

The herbal composite composition for alleviating or treating musculoskeletal disorders of the present invention (hereinafter, referred to as "the present invention composition") comprises: 1 step of preparing the herbal complex; a second step of preparing a mixed solution after adding the herbal compound to an extraction solvent; Step 3 of heating and distilling the mixed solution to obtain a concentrated extract; and 4 steps of filtering the extract to obtain the essence; it can be prepared by performing a process comprising a.

First, a description will be given of the herbal compound in the first stage.

The herbal complex includes lilac, angelica, cheonma, galgeun, oyak, hyangbuja, sokdan, safflower, licorice, and ginger.

Among the herbal complexes, the light sagebrush is a perennial plant of the Corydalis remotafisch family, and is a dried tuber of this plant. It is a tuber, usually unevenly flattened, and has a diameter of 0.5 to 1.5 cm. The outer surface is yellow to yellowish brown, and the quality is hard and crisp, with irregular net-shaped wrinkles. The cut side is yellow, keratinous, has a waxy luster, has almost no odor, and has a bitter taste. The light color indicates that alkaloids, including coridalin, which are the main components, have a sedative action, analgesic action, antispasmodic action, central anti-inflammatory action, blood pressure lowering action, and cardiac activity inhibitory action. is known In addition, it is used for postpartum vertigo, menstrual pain, clogged gihyeol, abdominal pain, arthralgia, neuralgia and other various pains, bruises, etc. plays a role

Angelica gigas root among the herbal complexes grows wild in deep mountains, but is mainly cultivated. The stem is about 1 to 2 m in height, and the root is used as a drug. The root is thick, contains milk, and has a strong fragrance. The root is about 20-25 cm long and about 1-5 cm in diameter. The main ingredients are essential oils, uterine excitatory ingredients, sucrose, and vitamin E. Its nature is mild, non-toxic, and it has a sour and sweet taste, but has a slightly bitter taste. It has a strong property of entering and acting mainly in the three spheres of the heart, liver, and spleen. It is also effective for menstrual cramps, and it is used in combination with Earl Potion, Yeonhosak, and Hyangbuja. In addition, it is effective for amenorrhea and menstrual irregularities. For symptoms such as palpitations, forgetfulness, insomnia, and mental instability, Angelica is used to purify and calm down. When the body looks dry and exhausted due to non-hyeol-heo, use the yanghyeol-geonbi with Angelica. When you are dizzy, your eyes are sore, your ears are ringing, and you have muscle spasms, you use Angelica for Yanghyeol and Yanggan. In addition, it is used for eohyeol, and it is known to improve and analgesic blood circulation for internal bleeding, blood flow stasis, swelling and pain caused by trauma such as bruises and sprains or vascular diseases.

Among the herbal complexes, the Gastrodia elata ( 天麻 ) is a monocotyledonous perennial parasitic plant of the Orchidaceae of the Orchidaceae family, which lives attached to the mycelium of a mushroom that grows on a rotten stump of an oak type, and uses tubers as medicines. It is known to have analgesic action and to suppress muscle contraction, stiffness, and tremor.

Among the herbal complexes, the kudzu is used as a drug in oriental medicine, and refers to the dried root of kudzu belonging to the leguminosae genus Pueraria genus or the root after removing the bark. Garlic root is known to contain isoflavonoids and triterpenoids, and has traditionally been used for the treatment of cardiovascular diseases and type 2 diabetes, as well as for antipyretic and sedative purposes. In addition, galgeun has the effect of increasing the blood flow and relieving contraction by expanding the muscle, so it is often used for the treatment of cardiovascular diseases such as high blood pressure. is known Although galgeun is low, it has relatively toxic side effects, so caution is required in how to use it.

Among the crude drug complexes, the oyak is a dried root of a plant belonging to the camphor family, 天台烏藥 ( Lindera strychnifolia Vill.), and the root is dug out in spring, washed with water, and dried in the sun. The taste is spicy and the nature is warm, and it is known to act on the gastric and nerves, to promote the circulation of Qi, to warm the stomach, to eliminate cold sores and to stop pain. . In addition, it has medicinal effects on acidosis, digestive disorders, diarrhea, menstrual pain, etc.

Among the herbal complexes, the Hyangbuja (Cyperus rotundus) is a perennial plant belonging to the family Cyperaceae, and grows in the sandy soil of the field banks, roadsides, and rivers. The whole plant is fragrant, and long underground stems grow sideways, and tubers are formed here and there. The flesh of the tuber is white and fragrant. Hyangbuja, a rhizome used in oriental medicine, is a long egg-shaped or spindle-shaped, pointed at both ends, 1-3.5cm in length, and 1cm in diameter, and sometimes has small stems at the tip. It is dark brown on the outside and often has brown, tough hairs. The quality is hard, and the broken side is reddish-yellow, and has a lustrous and aromatic flavor. Hyangbuja is collected in spring and autumn, dried, and then burned to remove hair. It mainly acts on the liver and samchogyeong (三焦经), but also works on menopause and biliary tract. Its nature is flat or slightly cold, non-toxic, spicy, slightly bitter, and sweet. The effects of Hyangbuja include Lee Gi Haeul (Relieves stress by facilitating the flow of Qi) and Landscape Relief (調经止通), and it is effective for pain caused by gas, especially menstrual pain and menstrual irregularities. is known

Among the crude drug complexes, the quickdan (Phlomis umbrosa TURCZ) is a dicotyledonous perennial plant in the order Lamiaceae, which is one of the herbal medicines and is originally a dried root of a hare flower. It is being used as a substitute. The name Sokdan was given because it heals fractures well. Its ingredients are alkaloids, essential oils, and vitamin E, and it has mild weak properties and a bitter taste. It is effective for back pain caused by weakness of the liver or kidneys and poor movement of the bones and muscles of the legs. It is also effective for arthritis and rheumatism.

Among the herbal complexes, the safflower (Carthamus tinctorius L.) is a dried safflower of the Asteraceae plant. It is a biennial herb native to Egypt and has no hairs, and the leaves are alternate and broad lanceolate. Flowers bloom in July-August, and have the shape of a thistle, but are reddish-yellow and hang one at the end of the main stem and one of the branches. The flower is used as a red dye and used for food dyeing, and in oriental medicine, it is used as a medicine for gynecological diseases and pain relief. Young shoots are eaten, and the seeds are used to make oil. Safflower is known to have medicinal effects that promote blood circulation, smooth the flow of meridians, remove eohyeol, and relieve pain.

Among the herbal complexes, the licorice (Glycyrrhiza uralensis FISCH) is used as a herbal medicine by drying the root, and is called licorice because its taste is sweet. Licorice contains sweet glycyrrhizin, glabriic acid, sucrose, glucose, liqueuritin, and licoricidin. Glycyrrhizin detoxifies diphtheria toxin, tetanus toxin, codeine hydrochloride, strychnine acetate, snake venom, pufferfish venom, etc. In addition, it was confirmed that glycyrrhizin has the effect of lowering blood pressure by lowering the cholesterol level in the blood, promoting the secretion of bile, and having antitussive and analgesic effects. As for the digestive system, it is known that the ulcer-suppressing action was shown as a result of ingesting licorice decoction after causing an ulcer in a dog. In addition, it showed a significant inhibitory effect on experimentally induced bladder stones, and it is known that it exhibited a significant inhibitory effect on ascites cancer and liver cancer in an anticancer activity test. Licorice is one of the most used medicinal herbs in oriental medicine.

Among the herbal complexes, the ginger is estimated to be native to southeastern Asia, and its rhizome has a fragrant and tangy taste, so it is used as a seasoning, flavoring, or medicine for food. The leaf-like stem grows up to 1 m tall. The leaves come out of the sheath surrounding the stem, and are 15-30 cm long and two long leaves are vertically opposite. The flowers are about 2.5 cm thick and 4 to 7.6 cm long, with overlapping green bracts with yellow edges, and each bract surrounds a small yellow-green/purple flower. Ginger contains about 2% essential oil, and its main ingredient is zingiberene, and zingerone has a tingling taste. The essential oil is extracted and used to make food and perfume. As a spice, ginger has a slightly bitter taste and is usually dried and ground for use in bread, confectionery, curry dishes, sauces, pickles, and ginger ale. The fresh rhizome of green ginger is used for cooking. The peeled rhizome of ginger is boiled and pickled in syrup, and in some regions, including Japan, it is sliced and eaten during meals to cleanse the palate. Ginger is a plant that is also used as a medicine to treat flatulence and abdominal pain.

The amount of herbal ingredients used in the herbal complex in step 1 used for preparing the composition of the present invention is based on 100 parts by weight of light pink, Angelica 　90 to 110 parts by weight, Chunma 80 to 110 parts by weight, galgeun 　70 to 100 parts by weight, Oyak 　70 ~ 100 parts by weight, 50 to 70 parts by weight of Hyangbuja, 50 to 70 parts by weight of safflower, 50 to 70 parts by weight of safflower, 50 to 70 parts by weight of licorice and 50 to 70 parts by weight of ginger, preferably 100 parts by weight of pale yellow About, Angelica 　 95 ~ 110 parts by weight, Chunma 85 ~ 100 parts by weight, Galgeun 　 75 ~ 95 parts by weight, Oyak 　 75 ~ 90 parts by weight, Hyangbuja 50 ~ 65 parts by weight, Sokdan 　 50 ~ 65 parts by weight, Safflower 55 ~ 70 parts by weight parts, 50 to 65 parts by weight of licorice, and 60 to 70 parts by weight of ginger, more preferably 100 to 110 parts by weight of Angelica 　 　 110 parts by weight, 90 ~ 100 parts by weight of Chunma 　 80 - 90 parts by weight, Oyak with respect to 100 parts by weight of light pink It is recommended to use 80 to 90 parts by weight, 60 to 65 parts by weight of Hyangbuja, 55 to 65 parts by weight of safflower, 60 to 70 parts by weight of safflower, 55 to 65 parts by weight of licorice, and 60 to 70 parts by weight of ginger.

Next, step 2 is a step of preparing a mixed solution by adding the crude drug complex of step 1 to the extraction solvent and sufficiently stirring at 25 to 30 ° C. In this case, 0.50 to 1 L of the extraction solvent per 1 kg of the herbal complex is used. It is preferable to use 0.55 to 0.80 L of the extraction solvent per 1 kg of the herbal complex, more preferably about 0.60 to 0.70 L of the extraction solvent per 1 kg of the herbal complex. At this time, if the amount of extraction solvent used is less than 0.5 L, the amount of extraction solvent used is too small, so the extraction solvent evaporates too quickly during distillation in step 3, which may make it difficult to extract the active ingredient. It takes too long and there may be uneconomic problems, so it is better to use it within the above range.

And, as the extraction solvent, it is preferable to use a mixed solvent in which water and an aqueous alcohol solution are mixed, preferably a mixed solvent containing water and an ethanol aqueous solution having a concentration of 50 to 70% by volume in a ratio of 1:0.2 to 0.5 by volume, More preferably, it is better to use a mixed solvent containing water and an aqueous ethanol solution having a concentration of 60 to 65% by volume in a volume ratio of 1:0.20 to 0.35 in terms of extracting the appropriate medicinal effect from the herbal complex.

Next, step 3 is a process of heating a mixed solution in which the herbal compound and the extraction solvent are mixed to extract the active extract ingredients in the herbal compound, and distilling the solvent to prepare a concentrated extract. Heating and distillation are the volume of the mixed solution It is preferable to perform so that Equation 1 below is satisfied.

[Equation 1]

10% ≤ {(volume of mixed solution before heating-volume of extract after heating)/volume of mixed solution before heating*100%} ≤ 20%, preferably 12.0% ≤ {(volume of mixed solution before heating-volume of extract after heating)/ Volume of mixed solution before heating*100%} ≤ 18.0%, more preferably 14.0% ≤ {(volume of mixed solution before heating-volume of extract after heating)/volume of mixed solution before heating*100%} ≤ 18.0%

At this time, the heating temperature is preferably 75 ~ 90 ℃, preferably heating to 80 ~ 85 ℃, if the heating temperature exceeds 90 ℃ ethanol component evaporates too quickly, the active ingredient may not be sufficiently extracted, heating If the temperature is less than 75 ℃, there may be a problem that the extraction of the active ingredient is rather difficult, and the process of preparing the extract through distillation is too long.

Next, step 3 is a process of obtaining a concentrate (or extract) from the concentrated extract by filtering the extract concentrated in step 2 by a general method used in the art.

The herbal composition for alleviating and/or treating musculoskeletal disorders of the present invention prepared in this way is applied as a herbal medicinal solution or injection, and is administered directly through the skin rather than orally, thereby relieving musculoskeletal disorders faster and more effectively than conventional oral therapeutic drugs. and/or treat.

In addition, the herbal medicinal solution and/or injection solution may further include a lubricant, emulsifier, suspending agent, preservative, etc. harmless to the human body.

And, to give a preferred example of acupuncture using the herbal composition for alleviation and/or treatment of musculoskeletal disorders of the present invention, the composition is used as an herbal medicinal acupuncture solution, the oriental medicinal acupuncture solution is immersed in the oriental medicinal acupuncture solution, and then acupuncture is performed on the affected area can do.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

[Example]

Example 1: Preparation of herbal composite composition for alleviation or treatment of musculoskeletal disorders

(1) Preparation of herbal complexes

With respect to 100 parts by weight of the pale yellow color, Angelica 105 parts by weight, Cheonma 97 parts by weight, Galgeun 85 parts by weight, Oyak 85 parts by weight, Hyangbuja 63.5 parts by weight, Sokdan 60.5 parts by weight, Safflower 65 parts by weight, Licorice 60 parts by weight, and Ginger 65 The herbal complex was prepared by mixing parts by weight.

Parts by weight of each of the above herbal complexes are based on dry weight.

(2) Preparation of extract

A mixed solvent was prepared in which distilled water and an aqueous solution of ethanol having a concentration of 62.5% were mixed in a volume ratio of 1:0.25.

Next, a mixed solution was prepared by mixing 0.625L of the mixed solvent per 1 kg of the herbal compound.

Next, the mixed solution was stirred and heated to 80 ~ 82 ℃, heating and distilling the solution until the volume of the mixed solution satisfies Equation 1-1 below to obtain a concentrated extract.

Next, the concentrated extract was filtered to prepare a herbal composite composition as the essence.

[Equation 1-1]

15.0% ≤ ≤ {(volume of mixed solution before heating - volume of extract after heating)/volume of mixed solution before heating*100%} ≤ 16.0%

Examples 2 to 3 and Comparative Examples 1 to 2

In the same manner as in Example 1 A herbal composition for alleviation and/or treatment was prepared, but the composition of the herbal complex was prepared as shown in Table 1 below, and then the herbal composition was prepared using the composition, respectively.

division
(parts by weight) pale color donkey celestial horse craving oyster rich man quick decision safflower licorice ginger Example 1 100 105 97 85 85 63.5 60.5 65 60 65 Example 2 100 100 100 90 90 70 65 70 55 70 Example 3 100 100 90 80 80 65 57 62 65 60 Comparative Example 1 100 60 60 85 85 63.5 60.5 65 60 65 Comparative Example 2 100 105 97 120 55 40 40 40 60 65

Preparation Example 1: Preparation of injection

The herbal composite composition prepared in Example 1 was mixed with sterile physiological saline (N/S) (Lot No.: 12062, source: JW Pharmaceutical) and diluted to a concentration of 35% by volume to prepare an injection.

Preparation Examples 2-3 and Comparative Preparation Examples 1-2: Preparation of injections

An injection was prepared in the same manner as in Preparation Example 1, but instead of Example 1, an injection was prepared using the herbal compositions of Examples 2-3 and Comparative Preparation Examples 1 and 2, respectively, and Preparation Examples 2-3 and Comparative Preparation Examples 1 and 2 were carried out, respectively.

Experimental Example 1: Measurement of inflammatory cytokine production inhibitory effect

In order to determine whether the herbal composite composition of Preparation Examples 1 to 3 and Comparative Preparation Examples 1 and 2 has an inhibitory effect on inflammation through TLR4 signaling, inflammatory cytokine production of the herbal composite composition in the RAW264.7 cell line stimulated with LPS The inhibitory effect was confirmed.

Specifically, the cultured mouse macrophage Raw264.7 was mixed with RPMI1640 culture solution containing 10% fetal bovine serum (FBS) and 1% antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) with 5% CO In the presence of ₂ , cultured at 37°C. Thereafter, 100 μl of each well of a 96-well microtiter plate was dispensed to the number of 1×10 ⁵ CFU cells and cultured overnight. Cells were treated with 0, 1, 5, and 10 μM of each of the herbal composites (Preparation Examples 1 to 3, Comparative Preparation Examples 1 and 2), respectively, and the positive control group was treated with only LPS 20 ng/ml and reacted for one hour. After that, stimulation with LPS and incubation for 16 hours at 37 ℃ in the presence of 5% CO ₂ by volume. After incubation, protein levels of TNF-α and IL-6 were confirmed by measuring absorbance at 540 nm using an ELISA kit (eBioscience, USA) and an ELISA reader (Molecular Devices, Sunnyvale, CA). The amounts of TNF-α and IL-6 were quantified using the standard protein included in the ELISA kit, and the measurement results are shown in Tables 2 and 3 below.

division TNF-α (ng/ml)
(inhibition rate %) herbal combination
treatment concentration positive control
LPS negative control
(0 μM) 1 μM 5 μM 10 μM Preparation Example 1 5.5 ng/ml
(100%) 5.6 ng/ml 4.7 ng/ml
(14.55%) 3.1 ng/ml
(43.64%) 2.1 ng/ml
(61.82%) Preparation 2 5.6 ng/ml
(100%) 5.5 ng/ml 4.9 ng/ml
(12.5%) 3.5 ng/ml
(37.5%) 2.7 ng/ml
(51.79%) Preparation 3 5.4 ng/ml
(100%) 5.7 ng/ml 5.0 ng/ml
(7.41%) 3.6 ng/ml
(33.33%) 3.0 ng/ml
(44.44%) Comparative Preparation Example 1 5.5 ng/ml (100%) 5.5 ng/ml 5.3 ng/ml
(1.85%) 4.8 ng/ml
(11.11%) 4.2 ng/ml
(22.22%) Comparative Preparation Example 2 5.6 ng/ml (100%) 5.6 ng/ml 4.9 ng/ml
(10.91%) 4.2 ng/ml
(23.64%) 4.0 ng/ml
(27.27%)

division IL-6 (ng/ml) (% inhibition) herbal combination
treatment concentration positive control
LPS negative control
(0 μM) 1 μM 5 μM 10 μM Preparation Example 1 3.6 ng/ml
(100%) 3.5 ng/ml 1.8 ng/ml
(50%) 0.9 ng/ml
(75%) 0.3 ng/ml
(91.67%) Preparation 2 3.5 ng/ml
(100%) 3.5 ng/ml 2.3 ng/ml
(34.29%) 1.3 ng/ml
(62.86%) 0.8 ng/ml
(77.14%) Preparation 3 3.5 ng/ml
(100%) 3.5 ng/ml 2.4 ng/ml
(31.43%) 1.5 ng/ml
(57.14%) 1.1 ng/ml
(68.57%) Comparative Preparation Example 1 3.7 ng/ml (100%) 3.8 ng/ml 3.5 ng/ml
(5.41%) 3.1 ng/ml
(16.22%) 2.4 ng/ml
(35.14%) Comparative Preparation Example 2 3.4 ng/ml (100%) 3.5 ng/ml 2.9 ng/ml
(14.71%) 2.6 ng/ml
(23.53%) 2.0 ng/ml
(41.18%)

Looking at Tables 2 and 3, it can be seen that when Raw264.7 cells subjected to LPS stimulation were treated with 1, 5 and 10 μM, Preparation Examples 1 to 3 had excellent TNF-α and IL-6 inhibitory effects. have.

In contrast, Comparative Preparation Examples 1 to 2 each showed a relatively TNF-α and IL-6 inhibitory effect when compared with Preparation Examples 1 to 3. Through this, it was confirmed that the herbal composites of Preparation Examples 1 to 3 had a very remarkable effect of inhibiting the production of inflammatory cytokines in RAW264.7 cells stimulated with LPS. Through this, it was confirmed that pain relief and improvement were possible through the anti-inflammatory effect on musculoskeletal diseases caused by inflammation.

Experimental Example 2: Measurement of the alleviation effect of chronic musculoskeletal disease through medicinal acupuncture

It was prepared without dilution of the herbal composite composition prepared in Example 1 as a medicinal acupuncture liquid for oriental medicinal acupuncture.

And, 20 patients with chronic musculoskeletal disorders such as shoulder pain, neck pain, sports injuries and aftereffects, traffic accident sequelae, sprains, herniated discs, piriformis syndrome, etc. For acupuncture, herbal medicinal acupuncture was applied to the affected area of pain.

Pharmacupuncture acupuncture was performed 3 times a week for 8 weeks, and the patient's musculoskeletal disease symptoms were checked every 4 weeks, and the results are shown in Table 4 below.

division 2nd week 4 weeks Week 8 very relaxed 6 8 14 ease 8 9 5 slightly relieved 4 2 0 no change 2 One One

Through the above experiment, it was confirmed that the pain of patients with musculoskeletal disorders could be greatly alleviated or improved through oriental acupuncture using the herbal medicinal acupuncture solution of the present invention, and it was determined that musculoskeletal disorders could be treated during acupuncture treatment in the long term. .

Claims (8)

Containing as an active ingredient the extract obtained by distilling the extract obtained by extracting the herbal complex containing licorice, angelica, cheonma, galgeun, oyak, hyangbuja, sokdan, safflower, licorice and ginger with an extraction solvent,
The crude drug complex is based on 100 parts by weight of pale yellow, Angelica 90 to 110 parts by weight, Chunma 80 to 110 parts by weight, Garlic root 70 to 100 parts by weight, Oyak 70 to 100 parts by weight, Hyangbuja 50 to 70 parts by weight, Sokdan 50 to 70 parts by weight, 50 to 70 parts by weight of safflower, 50 to 70 parts by weight of licorice and 50 to 70 parts by weight of ginger,
With respect to 100 parts by weight of the light yellow color, Angelica 90 ~ 110 parts by weight, Chunma 80 ~ 110 parts by weight, Garlic root 70 ~ 100 parts by weight, Oyak 70 ~ 100 parts by weight, Hyangbuja 50 ~ 70 parts by weight, Sokdan 50 ~ 70 parts by weight, Step 1 of preparing a herbal complex comprising 50 to 70 parts by weight of safflower, 50 to 70 parts by weight of licorice and 50 to 70 parts by weight of ginger;
a second step of preparing a mixed solution by adding the herbal compound to an extraction solvent and stirring;
Concentrate by distilling until the volume of the mixed solution satisfies the equation '10% ≤ {(mixed solution volume before heating - extract solution volume after heating)/mixed solution volume before heating * 100%} ≤ 20%' by heating the mixed solution Step 3 to obtain an extract;
A herbal composite composition for alleviating or treating musculoskeletal inflammation and pain disease, which is produced through a process comprising a; 4 step of filtering the concentrated extract to obtain the essence. [Claim 2] The herbal composite composition for alleviating or treating musculoskeletal inflammation and pain diseases according to claim 1, wherein the extract contains 0.50 to 1.00 L of the extraction solvent per 1 kg of the herbal compound. According to claim 1, wherein the extraction solvent is water and an aqueous solution of ethanol having a concentration of 50 to 70% by volume 1: 0.2 to 0.5 volume ratio of musculoskeletal inflammation and pain disease relief or treatment herbal composite composition for treatment. delete delete [Claim 2] An herbal medicinal acupuncture solution comprising the herbal composite composition for alleviation or treatment of musculoskeletal inflammation and pain disease according to claim 1. An injection containing the herbal composite composition for alleviating or treating musculoskeletal inflammation and pain diseases of claim 1 .
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