KR20130029284A - Composition of healthy foods for treatment of dementia - Google Patents

Abstract

PURPOSE: A health food composition for improving dementia is provided to cleanse pulmonary alveolus, supply oxygen to the brain, cleanse cerebral blood vessels, and intensify circulation function to cleanly increase blood in cerebral blood vessels. CONSTITUTION: A health food composition for improving dementia comprises an extract of two or more selected from pine rootlets, Tricholoma matsutake, pine nuts, Gastrodiae rhizome, and schizandra. The extract of mixture is comprised with 0.001-10 wt% of each thereof according to the total composition weight. Based on a dried weight of the composition mixture, 1-40 weight% of pine rootlets, 1-40 weight% of pine nuts, 1-40 weight% of Gastrodiae rhizome, or 1-40 weight% of schizandra are included. The health food composition may be in a form of one or more selected from a pill, granules, a soft capsule agent, a hard capsule agent, syrup, a beverage, a soft extract, a dry extract, a double boiled liquid, and an extracted double boiled liquid. The extract may be extracted by water, an organic solvent, or a mixture thereof. The organic solvent may be an alcoholic solvent of carbon number 1-4. The organic solvent may be one or more selected from methanol, ethanol, propyl alcohol, butyl alcohol, glycerine propylene glycol, and butylene glycol.

Description

Composition of healthy foods for treatment of dementia

The present invention relates to a health food composition for improving dementia, and more particularly, to a health food composition for improving dementia containing extracts of two or more kinds of mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schisandra chinensis. will be.

Recently, as the social structure changes rapidly, many medical expenses are incurred due to various diseases or diseases related to aging and aging due to stress diseases and acceleration of aging through social life, and this increase in medical expenses is leading to an increase in social costs. . Aging-related brain disease (dementia), among diseases related to aging, has recently been regarded as a representative disease among elderly diseases. In addition, the number of youths suffering from academic studies, entrance examinations due to various employment, social participation population preparing for various qualification tests for diversification of social participation, and education activities of middle-aged and seniors due to expansion of lifelong education have been expanded. There is an explosive increase in the number of people using it, which has led to a great deal of interest in dementia as the aging progresses. In order to solve this problem, various health functional foods, especially health food materials such as GABA (Gamma amino butyric acid) and phosphatidylserine (Phosphatidylserine), have been developed. Piracetam, a drug known to be effective in slowing the aging of the brain, is also available.

Stress is the source of all diseases, causing incongruity and circulatory disorders in the human body, acting as a cause of disease. When the body is under stress, secretion of epinephrine and norepinephrine is achieved by the hypothalamus-pituitary-adrenal (HPA axis) and the adrenal medulla and sympathetic nervous system. Increased corticotropin releasing factor secretion in the hypothalamus, increased adrenocorticotropic hormone (ACTH) secretion in the anterior pituitary gland, corticosterone and aldosterone in the adrenal medulla Increased secretion results in a stress response. Stress responses include increased blood pressure, increased heart rate, behavioral changes, blood flow from the gastrointestinal tract to skeletal muscle, decreased spleen weight, and decreased immunity due to reduced white blood cell count. If the stress is large and long-lasting, or if our body's stress response system is defective, it can lead to illness. Hypothalamic-pituitary-adrenal system (HPA axia) and hypersympathetic nerve conducting stress response can cause severe chronic diseases, depression, anorexia nervosa, reticulism, panic disorder, diabetes mellitus, hyperthyroidism, central obesity, hypertension It causes diseases like heart disease.

Excessive stress, such as noise, pollution, anxiety or depression affects the immune system, inhibits the body's ability to resist and causes various diseases such as infections, autoimmunity, and degenerative diseases of the brain. In addition, research is focused on the role of the immune system as well as the endocrine system. Cytokines, which play an important role in the immune system, are known to control stress response and cause stress disorders. That is, the release of cytokines in the central nervous system, including the hypothalamus and hippocampus, can cause local inflammation and act as a very detrimental factor to the human body. Exposure to prolonged stress can lead to the loss of nerve cells in the hippocampus and seriously affect learning and memory. However, studies on the relationship between the release of cytokines caused by stress and stress responses such as learning, memory loss and anxiety are ongoing, but the mechanisms and roles of these are not well known. It is virtually impossible to control the environmental stresses that an organism encounters in life, and given the reality that stress-related diseases soar, the understanding of the mechanisms of behavioral symptoms in response to the immune system in response to stress is what makes us stressed. It will help you a lot. Therefore, there is an urgent need for researches that reveal the central immunological mechanisms of stress and provide a theoretical background for the development of new drugs and treatments.

Alzheimer's disease can be caused by a variety of causes, but Alzheimer's disease is the most common cause of senile dementia, and the main cause of the disease is the accumulation of beta amyloid (Ab1-42) in the brain and its neurotoxicity. And Selkoe, Annu. Rev. Neurosci., 17, 489-517 (1994)] and other factors. After all, the root cause of dementia is the deactivation and death of neurons by various intracellular toxicity that occurs with aging. However, tacrine, cognex (donepezil, and aricept) known to date are known to enhance the action of acetylcholine, a neurotransmitter, which may alleviate some of the symptoms of dementia. Vomiting, diarrhea, etc.) are known problems.

In recent years, the emphasis on alternative medicine using natural products and other therapies replacing conventional medicine is increasing interest in natural products or health functional foods using natural products. Indeed, the development of products of natural products and natural products individually certified health food is very active, and thus, researches on fields that are not easily accessible with conventional medicine are being actively conducted. As mentioned above, as the social structure changes rapidly, it is effective to treat various diseases or disorders related to aging and aging through the stress of life and accelerated aging through social life, while having fewer side effects than conventional methods. Is the time to develop natural medicines or dietary supplements that are known to be even better, and through ongoing research, scientific validation and clinical testing of these methods are needed.

Therefore, the inventors of the present invention screened the natural products that are considered to be the most effective for the related diseases with reference to the traditional Chinese medicine and oriental medicine books in Korea, and tried to directly examine the effects of the natural products, among them to find a raw material for improving dementia The dementia improvement effect was verified. Through this method, we find effective natural products based on clinical efficacy and traditional Chinese medicine, find scientific evidence, develop health foods for improving dementia, and have excellent effects in the brain-related market including various age groups. It is expected to secure natural products and thereby secure market competitiveness.

Therefore, the present invention is to provide a healthy food composition for improving dementia by washing the alveoli, smoothing the oxygen supply to the brain, cleans the cerebrovascular vessels, and enhances the circulation function to clean the blood of the cerebrovascular vessels, The purpose is.

In one embodiment of the present invention, there is provided a health food composition for improving dementia containing an extract of two or more mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandra.

The extract of the mixture is contained in an amount of 0.001 to 10% by weight relative to the total weight of the composition, and the composition of the mixture is 1 to 40% by weight of pine twigs, 1 to 40% by weight, pine nuts 1 to 40, based on dry weight. It is contained in an amount of 1% to 40% by weight or Schisandra chinensis.

In addition, the health food composition is one or more formulations selected from the group consisting of tablets, granules, soft capsules, hard capsules, syrups, beverages, pills, soft extracts, dry extracts, water baths and extract baths to be.

In one embodiment of the invention, the extract is extracted with water, an organic solvent or a mixture thereof, the organic solvent is an alcoholic solvent having 1 to 4 carbon atoms, preferably methanol, ethanol, propyl alcohol, butyl alcohol, glycerin propylene At least one selected from the group consisting of glycol and butylene glycol.

The composition according to the present invention is effective in improving dementia by rinsing alveoli, facilitating oxygen supply to the brain, cleans cerebrovascular vessels, and strengthens circulatory functions to clean the blood of cerebrovascular vessels, , Easy to ingest. In addition, there is no risk when drinking using extracts derived from natural, safe to the human body has the advantage that there is no side effect even when taken for a long time.

The present invention is described in more detail as follows.

Pine leaf, pine, pine, cheonma or schisandra chinensis as an active ingredient of the health food composition for improving dementia of the present invention has the following characteristics.

Pinus densiflora ) Stem is 35m in height and 1.8m in diameter, and bark is reddish brown but the bottom is black brown. Needle leaves are put together two by 8 ~ 9cm long and 1.5mm wide. After two years, the needle leaves fall down. Flowers bloom in May, and male flowers hang on the bottom of the new branch, yellow, 1cm long, oval. Female flower hangs on the end of new branch and is purple and 6mm long egg shape. Fruits are egg-shaped, 4.5cm long, 3cm in diameter, 70-100 pieces of fruit, and ripen yellowish brown in September-October the following year.

Pine is slowing aging, bactericidal, anti-inflammatory, cough-stopping, nourishing, convulsive anti-inflammatory, hemostatic, bile secretion promoting, longevity, anticancer, blood pressure lowering, nicotine excretion, Blood cholesterol release, promotion of blood circulation, prevention of high blood pressure, cold, prevention of epidemic meningitis, chronic bronchitis, gastric and duodenal ulcer, fatigue, chronic constipation, dysentery not clear after several months, scurvy, malnutrition in children, Anti-aging and prevention, pediatric eczema, dermatitis caused by pediatric diapers, traumatic bleeding, scrotum's swelling, taste, swelling due to strokes, gum pain, tongue stiffness, gusts, headaches, blisters, backwinds, scabies, bruises, sprains Itching, skin itching, myocardial infarction, weak heart or heart disease, cerebral anemia, arteriosclerosis, cerebral infarction, constipation, hemorrhoids, vaginitis, allergic rhinitis, acute gastritis, chronic alcohol Poisoning, fundus bleeding, bad breath, stroke, all symptoms of stress, rheumatism, pulmonary tuberculosis, conjunctivitis, ulcers, oralitis, neuralgia, back pain, shoulder swelling, gum disease, alveolar bone disease, tonsils swollen, toothache, insomnia, fatigue Boredom, bully, cholesterol suppression, eczema, bruises, swelling, bleeding, heart attack, swelling and heartburn , Symptoms of darkening of the ears, tooth decay, sprouting of new flesh, moisturizing cardiopulmonary, thirst, brightening ears and eyes, neurodermatitis, pediatric baldness, tears in the liver, when the child does not keep his mouth closed Long-term symptoms, gynecological malaise, hair and eyebrow dropout, dizziness, alcohol poisoning, nervous breakdown, vitamin C deficiency, epidemic meningitis, the prevention and treatment of pandemic cold, nutritional number Species, burn, frostbite, customs window, hair promotion, ileum stabilization, customs bone pain, intestinal bleeding, chronic dysentery, teasickness, long-standing onset, gold lance, customary arthritis, veil wounds, pediatric cranial invasion, limb pain, It promotes circulation of the muscles, muscle pain, meridians through, muscle relaxation, bruises caused by blood, rejuvenate the five intestines, etc.

Among them, the roots of pine are 75% α-pinene, camphor, dipentene, α-terpineol, camphor, and p-mentanol. The taste is bitter and the nature is warm and nonpoisonous. It is known to nourish Oro and to treat stiff tooth pain, musculoskeletal pain, upper hand pain, and tooth decay.

Musculoskeletal pain is taken with Songjugeun (Songjugeun) with white wine (白酒). [Honam Pharm] In the blood of blood and bruises, scrape off the rough skin of mami pine root, dry it on a low heat, stir-fry it, and finely powder it. Take it with warm liqueurs. [Gangseo Minchocho Drugstore] Wind chimney and tooth decay shall be cut into pieces of Yusong-geun roots of Mami-song-myo, cut into pieces, and taken with pork pork meat and 4 minutes before bedtime. . [Gangseo folk herb medicine laboratory] (Written / Herb Researcher & Adjunct Professor of Alternative Medicine, Dong-A University)

Tricholoma nauseosum ( Blytt ) Kytovuori ) is an edible fungus of the genus Cortis fungus , Pleurotus eryngii .

The cluster may promote blood circulation and prevent arteriosclerosis and heart disease. The dietary fiber in the cluster is attached to cholesterol and bile acids, which are excreted together to reduce blood cholesterol levels and improve blood circulation to prevent atherosclerosis, heart disease, diabetes, and hyperlipidemia. Matsutake is effective for tonsils, mastitis, and dehydration, and also helps to increase appetite. Clusters are rich in protein and vitamins, and are good for tonsils, mastitis, and dehydration. This helps the stomach and intestines function, promotes appetite and diarrhea. In addition, it promotes blood circulation and can be used when hands and feet are numb and have no power or knee pain.

The cluster helps digestion and improves obesity. The pine mushroom contains digestive enzymes of starch and protein, so the foods with pine mushrooms are well digested, so it is very good for people with high fever or obesity.

Matsutake has anti-cancer effects and prevents cancer. In clusters, there is a 'single protein' that kills cancer cells, which has the highest anti-cancer effect among mushrooms known to date, stabilizes the human heart, cures inflammation, and suppresses tumors.

Among the main components of Matsutake mushroom, free fatty acids have 82.6 ~ 86.7% of fatty acid, which is very high compared to other foods, making it a health food. In addition, the inorganic content is contained in a large amount compared to other mushrooms in comparison with other mushrooms. Among them, the representative mineral potassium of common mushrooms contained 10 times of oysters, about 40 times of mushrooms, and about 3 times of thirsty mushrooms, and iron also showed a tenfold difference from other mushrooms.

Pinus koraiensis Zieb meat Eucc ) is an evergreen tree belonging to the pine family and refers to the fruit of Korean pine or Korean pine. The leaves are similar to pine needles but greener and thicker. Normally pine means two-leaf pine, but pine tree is also known as pine tree because pine needle-shaped leaves come out in five places. Medicinal pine nuts are called Haesongja (海松 子), also known as pine tree (五 葉松), songza (松子), and white porcelain (柏 子).

Like pines other than pine trees, pine nuts are formed in pine cones. On average, about 100 grains are deleted in pine cones of 6 ~ 10cm in diameter and 9 ~ 15cm in length. Pine nuts are not only high in calories, but also rich in B vitamins and contain more iron than hodo or peanuts, which are effective against anemia. Pine nuts have high content of unsaturated fatty acids and excellent amino acid composition.

Pine nuts are known to be rich in unsaturated fatty acids such as oleic acid, linoleic acid, and linolenic acid, which make skin elasticity, lower blood pressure, make skin smooth, lower blood pressure, and help stamina. In addition, it is composed of niacin, sodium, protein, sugar, retinol, beta-carotene, vitamin A, B, C, E group, dietary fiber, zinc, folic acid, phosphorus, lipid, iron, potassium, calcium and the like.

Gastrodia elata ) is a perennial herb that grows in deep mountains all over Korea. The growing environment grows in decaying oaks in damp stone cracks and shade or half shades. The height is 60 ~ 90㎝, there is no leaf, and the shape of fish scale is short, and the yellowish brown stem is raised. Roots are 10 ~ 18㎝ long and 3.5㎝ in diameter, extending in a long oval. The flower is yellowish brown with the length of 10 ~ 30㎝ attached to the stem. Fruits run from September to October, and seeds are dusty and small in the black ovary.

Chunma is used as a preventive and therapeutic agent for hypertension, stroke, insomnia, sports, headache, dizziness, paralysis and neurological diseases. It also has effects on promoting adult disease and liver function such as diabetes, relieving fatigue and stress. It is also effective in increasing learning and memory, and is often used as the main ingredient of Chongmyeongtang. The clinical efficacy of Chunma is reported to be mainly effective in the symptoms of hypertension, headache, paralysis, neurological diseases, diabetes and other diseases, such as stress, fatigue. The origin of this effect in scorpions is presumably related to the antioxidants contained in scorpions. Crude protein content was 5.4%, crude ash 2.6%, crude fat 3.6%, crude fiber 3.3%, moisture 8.1%, and starch was the highest with 77%. Chunma has higher protein content than other crops and is composed of unsaturated fatty acids. If Chunma is compared to wild ginseng, its appearance and calling name may be similar to that of bellflower, but its efficacy is large. Chunma is known to have higher levels of calcium, magnesium, and potassium than other foods, and has recently been recognized for its effectiveness in treating osteoporosis, preventing acidification of body fluids, improving learning ability, and reducing blood cholesterol. It is a plant that is taking its place as a food.

Schisandra chinensis ( Turcz .) Baill ) Is a deciduous deciduous tree that grows in 200 ~ 1,600m above sea level and grows a lot in Jirisan, Sokri and Taebaeksan. The height is 6 ~ 9m, the leaves are 7 ~ 10㎝ long, 3 ~ 5㎝ wide, hairy on the back vein, small tooth teeth on the edge, and wide oval. The flower is slightly reddish yellowish white, about 1.5㎝ in diameter, and blooms one by one on the leaf axilla of 3 ~ 5 new branches. Fruits are sour and strong in red in August ~ September, 0.6 ~ 1.2㎝ in length, and several hang down under grape clusters.

Schisandra chinensis is effective for relieving and asthma, sweating and producing essence, which is effective for small thirst. Oil well (遺精), urination (遺尿), frequent urinating symptoms, dysentery, diarrhea, heart beats and sleep can not be used to dream a lot of symptoms. It is effective in reducing memory and concentration.

Pharmacological action: cerebral cortical excitability, blood pressure lowering, expectoration, antitussive action, respiratory excitability, cardiac action, glucose metabolism and glycolysis, cellular immune function enhancement, uterine excitability, bile secretion, gastric secretion control Action, antibacterial activity has been reported. In addition, it regulates the physiological function in the cardiovascular system, improves blood circulation disorders, increases the responsiveness of the central nervous system, strengthens the brain function and stabilizes the mental function to prevent dementia, and is also good for examinees who need thinking and concentration.

In Chinese medicine, Schisandra chinensis is effective in helping lungs. It is very effective for coughing and asthma in patients with chronic bronchiectasis, and it is effective for coughing and sputum in people who work in an airy environment. When a child coughs frequently, Schisandra we have a lot of benefits if you continue to feed the water. In addition, blood sugar levels are also helpful for quenching thirst in diabetics. In particular, because of its excellent anti-fatigue effect, healthy people eat Schizandra in about 30 minutes, which increases their mental and physical activity and makes them feel less tired. This effect lasts 3 to 4 hours. When you catch a cold, Schisandra chinensis marinated in sugar and eating the concentrate can help prevent colds and boost immunity, and you can expect the effects of autumn remedies to stock up your stamina.

 Extract of a mixture of at least two kinds of herbal materials selected from the group consisting of the above pine twigs, pine, pine, scorpion, schizandra, etc. may be included in an amount of 0.001 to 10 parts by weight based on the total composition. When the extract is included in less than 0.001 parts by weight, dementia improvement effect may be insignificant, when included in more than 10 parts by weight may reduce the stability of the formulation, may not be good taste and aroma when prepared as health food have.

Extracts of two or more mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandrae are contained in an amount of 0.001 to 10% by weight, based on the total weight of the composition, containing less than 0.001% by weight of the extract. If it is, the effect for improving dementia may be insignificant, and if it is contained in an amount of more than 10% by weight, the stability of the formulation may be lowered, and taste and aroma may not be good when prepared as a health food.

The extract of the mixture is a natural natural product that is recovered by filtering a solution obtained by adding a hydrophilic organic solvent such as methanol, ethanol or the like, or a mixture of a hydrophilic organic solvent and water and stirring at predetermined time intervals while depositing for a predetermined time. It can be obtained according to the extraction method. Preferred extraction solvents include alcohol solvents having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, butyl alcohol, glycerin propylene glycol and butylene glycol, water and mixtures thereof. The amount of the extraction solvent added to the natural material may be about 1 to 50 times the total volume of the natural material.

In one embodiment of the invention, it is preferable to use a solvent selected from the group consisting of anhydrous methanol, hydrous methanol, anhydrous ethanol, hydrous ethanol and mixtures thereof as a natural extractant. In another embodiment of the present invention, it is preferable to use a mixed solvent of one of the aforementioned methanol solvents and one of the aforementioned ethanol solvents.

At the time of extraction, the amount of the extraction solvent added is preferably 5-20 ml per 1 g of the dry weight of the natural product material. Extraction conditions of the natural product material may be used by immersion extraction for 1-7 days at room temperature or 1-5 hours at a heating condition of 50-95 ℃, but is not limited to these general solvent extraction used in the art All of the methods can be applied.

The formulation of the health food composition containing the extract of two or more mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandra mentioned above is not particularly limited and may be appropriately selected as desired. For example, the health food composition of the present invention may be prepared in the form of tablets, granules, soft capsules, hard capsules, syrups, beverages, pills, soft extracts, water baths and the like. More specifically, the health food composition of the present invention may be prepared in the form of tablets, granules, soft capsules, hard capsules, syrups, beverages, pills, soft extracts, dry extracts, extract liquid bath.

In addition, the health food composition of the present invention can be used by appropriately formulating excipients, water, ethanol, sweeteners, flavorings, pigments, preservatives, and the like for each formulation in addition to the components described above for the formulation of general foods. .

A composition comprising a substance according to the invention provides a dietary supplement that improves dementia. Examples of the food containing the substance according to the present invention include various foods, beverages, gums, teas, vitamin complexes, and health functional foods. It can also be added to food or beverages.

The health food composition of the present invention has no particular limitation on other ingredients besides the substance according to the present invention as an essential ingredient, and may contain additional ingredients such as various flavors or natural carbohydrates, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a flavoring agent other than the above-mentioned, a natural flavoring agent, taumartin, a stevia extract, for example, rebaudioside A, glycyrrhizin, etc .; And synthetic flavoring agents such as saccharin, aspartame and the like. The proportion of such natural carbohydrates is generally about 1 to 10 g, preferably about 5 to 6 g per 100 ml of the composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, these Examples and Experimental Examples are not intended to limit the scope of the present invention only to aid the understanding of the present invention.

Manufacturing example  1. Preparation of Mixed Extract

To a mixture of 30% by weight pine root, 20% by weight, 20% by weight pine, 20% by weight, 15% by weight and 15% by weight Schisandra chinensis, 80% ethanol was added to a volume of twice the amount of the mixture and cooled for 3 days. Later, the solution was collected and filtered through filter paper, and then the solvent was removed using an evaporator, dried in a freeze dryer, dried for at least 48 hours, and powdered.

Example  1. Preparation of health food for improving dementia

To prepare a health food for improving dementia in the composition of Table 1 below. The units in Table 1 below are by weight.

Example  2. Preparation of health food for improving dementia

To prepare a health food for improving dementia in the composition of Table 1 below. The units in Table 1 below are by weight.

Comparative example  1. Preparation of health food for improving dementia

To prepare a health food for improving dementia in the composition of Table 1 below. The units in Table 1 below are by weight.

Ingredients Content (% by weight) Example 1 Example 2 Comparative example Pine Root, Pine, Pine, Cheonma, Schisandra chinensis extract 1.0 10.0 - Liquid fructose 10.0 10.0 10.0 Arabian sword 1.5 1.5 1.5 Sugar 0.5 0.5 0.5 Citric acid 2.5 2.5 2.5 Soy lecithin 1.5 1.5 1.5 oligosaccharide 3.0 3.0 3.0 Sodium citrate 0.1 0.1 0.1 Polydextrose 1.0 1.0 1.0 Antioxidant Suitable amount Suitable amount Suitable amount Spices Suitable amount Suitable amount Suitable amount Pigment Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Purified water Suitable amount Suitable amount Suitable amount

Experimental Example  1. Animal Experiment

Experimental Example  1-1.Preparation of Test Solution

200 mg of pine root, pine, pine, cheonma and Schisandra chinensis extracts were dissolved in 200 mg of physiological saline containing 1.0% of polyoxyethylene hydrogenated castor oil (Cremophor RH 40 ^® , BASF). Injection formulations containing a concentration of was prepared. The test was carried out using a general forgetfulness model, and the injection preparation was confirmed to have an improvement in memory disorder through animal testing.

Experimental Example  1-2. Experimental animal preparation

C57BL / 6 male mice (5-6 weeks old) were prepared.

Experimental Example  1-3. Experimental Device Preparation

A step-through type passive avoidance test plate (self-made) consisting of a bright room (15 × 10 × 10 cm) and a dark room (20 × 15 × 12 cm) was produced. At the top of the room, the room is illuminated with a 20-W fluorescent lamp. The two chambers were divided into entrances that could be opened and closed up and down. At this time, the grid of the dark room was connected to the electrical stimulation device to give an electric shock to the leg of the mouse.

Experimental Example  1-4. Impact test

The mouse was placed in a clear room and after about 10 seconds the door was opened to allow the mouse to enter the dark room. Immediately after the mouse entered the dark room, the entrance was closed, and after about 5 seconds, an electric shock of AC 45-50V was applied to the mouse for 1 second, and the mouse was immediately taken out of the dark room.

Experimental Example  1-5. Reproduction test

After 24 hours of the impact test, the mice were placed back in the clear room and the same procedure was repeated except that no electric shock was applied. The time it takes for the door to open itself and the mouse to enter the dark room is measured up to 300 seconds.

Experimental Example  1-6. Induced forgetfulness by electric spasms shock

Immediately after the impact, both ears of the mouse received an electric spasm shock (AC 100-140 V, 0.5 sec).

Experimental Example  1-7. Administration of Test Compound

The test drug was divided into five groups, the negative control group and the positive control group {2- (10-hydroxydecyl) -5,6-dimethoxy-3-methyl-2,5-cyclohexadien-1,4-dione)} and Extracts of two or more mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandra according to the present invention were prepared and suspended in physiological saline containing 5% gum. The prepared drug was administered intraperitoneally 30 minutes before the reproduction test in the forgetfulness-induced test by electroconvulsive shock.

Experimental Example  1-8. result

       Table 2 shows the results of the effects on forgetfulness caused by electric cramp shock.

division Dose of Active Ingredient (μg / kg) Number of animals used Retainer (sec)
Mean ± SE % Improvement No treatment - 10 292.4 ± 3.2 - Electric Spasm Shock Treatment - 10 81.0 ± 10.1 Negative control group
(Blank) - 10 83.5 ± 6.1 1.2 Positive control group
(Idebenon) 80 10 108.1 ± 16.8 12.8 Mixed extract 10 10 131.1 ± 13.8 23.7 Mixed extract 50 10 143.2 ± 23.4 29.4

*: Significant difference (P <0.05) with respect to the electroconvulsive shock treatment group.

(1)

As shown in Table 2, it was confirmed that the compound of the present invention showed a marked improvement effect on experimentally induced forgetfulness compared to cerebral metabolism activator (idebenone) and the comparative example.

Experimental Example  2. Clinical Trials of Memory Improvement

Clinical trials were conducted to investigate the memory-enhancing effects of dementia-enhancing health food compositions containing extracts of two or more mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandra. The subjects were aged over 60 years old and drink about twice a day. Thirty subjects were divided into three groups of ten people each to drink the products of Example 1, Example 2, and Comparative Example. While drinking the experimental product, it was decided to prohibit any dementia-related treatments (such as therapy or medication). The symptom was evaluated after drinking, and the results of the clinical trial for improving memory are shown in Table 3 below.

ratio (%) Example 1 Use Group Memory is greatly improved 0 Slightly improved memory 40 No difference 50 Memory deteriorates 10 Example 2 Use Group Memory is greatly improved 10 Slightly improved memory 60 No difference 30 Memory deteriorates 0 Comparative Example Use Group Memory is greatly improved 0 Slightly improved memory 0 No difference 90 Memory deteriorates 10

From the results of Table 3, it was found that the health food composition for improving dementia of the present invention is helpful in improving dementia by improving memory.

While the present invention has been described with reference to exemplary embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. You will know. In addition, many modifications may be made to adapt a particular situation and material to the teachings of the invention without departing from the essential scope thereof. Accordingly, it is intended that the invention not be limited to the particular embodiment disclosed as the best mode contemplated for carrying out this invention, but that the invention be construed as including all embodiments falling within the scope of the appended claims.

Claims (7)

A health food composition for improving dementia comprising extracts of two or more kinds of mixtures selected from the group consisting of pine twigs, pine, pine, cheonma and schizandra. The method of claim 1,
Extract of the mixture is dementia improvement health food composition, characterized in that it is contained in an amount of 0.001 to 10% by weight relative to the total weight of the composition. The method of claim 1,
Improvement of dementia, characterized in that the composition of the mixture is contained in an amount of 1 ~ 40% by weight, pine tree 1-40% by weight, 1-40% by weight, 1-40% by weight or Schisandra chinensis based on dry weight Health food composition for. The method of claim 1,
The health food composition is one or more formulations selected from the group consisting of tablets, granules, soft capsules, hard capsules, syrups, beverages, pills, soft extracts, dry extracts, water baths and extract baths Health food composition for improving dementia. The method of claim 1,
Dementia improvement health food composition, characterized in that the extract is extracted with water, an organic solvent or a mixture thereof. The method of claim 5, wherein
Dementia improvement health food composition, characterized in that the organic solvent is an alcoholic solvent having 1 to 4 carbon atoms. The method according to claim 6,
The organic solvent is a health food composition for improving dementia, characterized in that at least one selected from the group consisting of methanol, ethanol, propyl alcohol, butyl alcohol, glycerin propylene glycol and butylene glycol.