KR101930277B1 - Composition for Preventing and Improving Hangover - Google Patents

Abstract

The present invention relates to a health functional food composition for preventing and alleviating hangover and a pharmaceutical composition for preventing or treating alcoholic fatty liver comprising an extract of Pueraria lobate, Poria cocos, Amomum villosum, Cinnamomum cassia, Lonicera japonica. The health functional food composition for preventing and alleviating hangover and the pharmaceutical composition for preventing or treating alcoholic fatty liver can be safely used using a plant extract, is easy to be taken, can be stored for a long period of time, accelerates the metabolism of alcohol in vivo to rapidly lower the blood alcohol concentration when administered before or after alcohol consumption, thereby exhibiting a hangover relieving effect or preventive effect or therapeutic effect of alcoholic liver diseases.

Description

[Composition for Preventing and Improving Hangover]

A health functional food for preventing and improving hangover, and a pharmaceutical composition for preventing or treating alcoholic fatty liver.

Alcohol ingested by alcohol is easily absorbed in the gastrointestinal tract, of which only 2 - 10% is excreted through the lungs and kidneys, and the remaining 90 - 98% is mainly oxidized in the liver. Alcohol from the liver is mainly oxidized through the alcohol dehydrogenase system in the cytoplasm, but it is also metabolized through the ethanol oxidizing system in the microsome. Alcohol affects carbohydrate and fat metabolism, causing metabolic disturbances such as hyperlipidemia and hyperlipidemia, inhibiting the oxidation of fatty acids in hepatocytes, increasing the synthesis of triglycerides, forming fatty liver, and even progressing to cirrhosis. Alcohol also passes through the walls of the blood vessels causing mental confusion and cranial nerve impairment.

Hangover refers to headache, diarrhea, anorexia, nausea, vomiting, chills, and cold sweating that occur after drinking alcohol. Objective symptoms include cognition, decreased exercise capacity, hematological changes, and changes in hormones. The cause of hangover is known as dehydration, toxicity of alcohol and alcohol metabolites (acetaldehyde, formaldehyde, acetone, etc.), and nutrient deficiency (blood sugar, vitamin, mineral deficiency) Drinking temporarily causes hangover such as throbbing, headache, thirst, motion sickness, gastrointestinal disorder, and diarrhea, and may cause chronic diseases such as fatty liver and cirrhosis. Headaches during hangover are due to the expansion of brain blood vessels. Thirst and dehydration are caused by the diuretic effect of ethanol, which is produced by the suppression of the secretion of vasopressin, an antidiuretic hormone secreted by ethanol in the pituitary gland. In addition, digestive organ abnormalities such as gastrointestinal disorders are caused by an increase in gastric acid secretion, and the main cause is toxicity of ethanol or acetaldehyde accumulated in hepatocytes. In addition to the mechanism of ethanol degradation, toxicological studies of ethanol have been done in various ways. It has been reported that the toxicity of ethanol is not only observed neurologically but also genetically influenced (J. Caballeria, et al Life Sci., 41: 1021-1727, 1986).

After drinking, alcohol is absorbed in small amounts in the esophagus and oral mucosa, about 10% in the stomach and 90% in the small intestine. Alcohol is metabolized through three pathways. When the concentration of ethanol is low, the action of alcohol dehydrogenase and acetaldehyde dehydrogenase, which are present in the gastrointestinal tract or liver, It is metabolized to acetaldehyde and acetic acid by the existing microsomal ethanol oxidizing system (MEOS), and then by the action of catalase present in the peroxisome, carbon dioxide (CO ₂ ) and Water (H ₂ O). When an appropriate amount of alcohol is introduced, the metabolic system described above smoothly works, so that all the symptoms caused by alcohol do not occur. However, when a large amount of alcohol is introduced, the balance of the metabolism system is destroyed and the body homeostasis can not be maintained. In this situation, liver is the most affected organ, and in the long term, various liver dysfunctions such as fatty liver and liver cirrhosis occur, and in the short term, hangover symptoms such as headache or dizziness, loss of concentration, sore throat and indigestion are caused .

Studies on hangover are mostly conducted through animal experiments in Korea, and substantial research is being conducted mainly on human trials abroad. In Japan and the United States, Europe and the United States are actively engaged in the study of toxic substances, toxic agents, and poisoning symptoms of ethanol (methanol). Particularly, since the structure of tetrahydroisoquinoline or tetrahydro-β-carbolines produced as a result of in-vivo metabolism of ethanol is similar to that of morphine, withdrawal symptoms are induced, do. Recently, methanol metabolism and formaldehyde, which are contained in a small amount in alcohol (ethanol), have been pointed out as the cause of hangover. Many reports that alcohol hangover is due to alcohol metabolism, such as acetaldehyde and acetone, (Moser J, Bagchi D, Akubue PI, Stohs SJ. Alcohol Alcohol. 28: 287-295, 1993). Most of the alcohol consumed is oxidized and metabolized, so the amount that is not metabolized and excreted is only 2% of the intake amount, and even if the large amount is consumed, it is less than 10%. Therefore, since the acute toxicity by alcohol is attributed to the modification products of ethyl alcohol, acetaldehyde, acetone and other alcohol metabolites, lowering the blood alcohol concentration after drinking may shorten the stay time in the acute poisoning state, It can be said that it has importance.

In recent years, researches and experiments have been conducted on substances that can alleviate the toxicity of ethanol or inhibit the expression of toxic substances. Especially, a lot of health foods for hangover habit using materials are being developed. Studies on the existing studies have shown that extracts containing extracts obtained from different kinds of raw materials such as persimmon shells, parsley, persimmons, omija, mung bean, chestnut, chestnut, (Korean Unexamined Patent Publication No. 2002-81995) ", " a low-grade hepatotoxic and hangover-removing activity isolated from Hovenia dulcis, " Alcohol-insoluble extract fractions and polysaccharide materials and compositions containing them (Korean Unexamined Patent Application Publication No. 2002-64151), "" Hobbenodolinol, an active ingredient of Jungfujang, a process for producing the same and an alcohol- (Korean Patent Publication No. 2002-86963) ", as well as a variety of plants derived from various plants such as rice gruel (Kurume), alder tree, oak wood vinegar, There have been reports that the hangover effect. As a raw material, silk fibroin (Korea registered patent no. 0528740), ogapi, nogeun, corn, cherry, low river (Korea registered patent No. 0528388), wormwood, gugija, health, dermis, lotus root, (Korea registered patent No. 0512912), astaxanthin, asparaginic acid (Korean registered patent No. 0520985), persimmon leaf, persimmon (Korean registered patent No. 0504351), dermis, Baekbokryeong, (Korea registered patent No. 0496524), Duck (Korean Patent No. 0462329), soybean, bean sprouts, grape seed (Korean Patent No. 455075), active ingredient ingredient of apricot juice, asparagus, green tea, onion, (Korean Registered Patent No. 0452128), radish, black tea, persimmon leaf, persimmon leaf, Saururus chinensis (Korea registered patent No. 0451013), decaccinol (Korean registered patent No. 0448680) (Korean Registered Patent No. 0446061), white mugwort, audi, tosaja, gal (Korean Registered Patent No. 0442771), Pine Needle (Korean Patent No. 0412425), Garnet, Big Thistle Seed, Taurine, Ulgum (Korean Patent No. 0392876), Naringin , Naringenin (Registered Patent No. 0375047), Duckwood, and Rana (Korean Patent No. 0181168). The raw materials used in the other patents include horseradish, mushroom, green tea catechin, ogapi, palm cactus (Paekyukcho), Chunma, Rootstock mushroom, Chopstick, Propolis, However, to date, there have been developed hungable beverages, food, and chewing gums using the above materials, but the effect is not remarkably excellent. In addition, a drink containing some herbal medicine may cause general boredom, abdominal bloating, vomiting, abdominal pain, etc., and there is a problem that it is sold at a high price by using an expensive herbal medicine, and various changes , Only some items have an effect or a small effect.

The liver is the most vital organ of the human body organs. It is a chronic or chronic disorder caused by various causes such as excessive intake of food or alcohol containing fat, harmful substances such as virus infection and various drugs It can be caused by fatty liver, hepatitis, jaundice, cirrhosis and liver cancer. In particular, excessive intake of fat or alcohol through the food causes fat accumulation in the liver tissue where lipid accumulates. In this case, glutamate-oxaloacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT) and γ-glutamyl transferase . As a result of the chronic ethanol consumption, the metabolism results of ethanol by the alcohol dehydrogenase (ADH) and the aldehyde dehydrogenase (ALDH) increase the ratio of NADH / NAD + in the cell and the intracellular NADH / NAD + ratio in relation to the mechanism of hyperlipemia and fatty liver, (Murray RK et al., Haper's Biochemistry, Appleton & Lange, Connechcut, USA, 3rd ed., P260 (1993)) increase the fatty acid oxidation and TCA cycle activity in hepatocytes.

Alcoholic liver disease is divided into alcoholic fatty liver, alcoholic hepatitis and liver cirrhosis. Double fatty liver is the most common, but occasionally these three diseases may overlap and their severity is mainly related to alcohol consumption. Excessive alcohol consumption induces lipid accumulation in the liver tissue, which increases the GOT (glutamate-oxaloacetate transaminase), GPT (glutamate-pyruvate transaminase) and γ-GTP (γ-glutamyl transferase). This lipid accumulation in the liver increases the synthesis of triglyceride in the liver, induces obstacles in the secretion process of lipoprotein synthesis, increases liver inflow of fat liberated from peripheral adipose tissue, while lowering fatty acid oxidation process . Alcoholic hepatitis usually refers to the inflammatory reaction and fibrosis of the liver as well as the adipocyte infiltration. Furthermore, when the liver cell necrosis and the degree of deposition of the collagen increase, the nodule develops into alcoholic cirrhosis. It is easy for anyone to understand that alcoholic liver disease increases the risk of incidence as the number of alcohol drinks increases and the longer the duration of alcohol consumption, the more actual results are supported.

Accordingly, the present inventors have conducted studies to develop a novel composition which has no side effects on the human body and has an excellent effect on hangover symptoms caused by alcohol and alcoholic fatty liver, and it has been found that when extracts of bamboo, bamboo shoots, cinnamon, The composition of the present invention has the effect of rapidly reducing the blood alcohol concentration without toxicity and side effects, thereby completing the present invention.

Korean Patent Publication No. 10-2017-0052868 Korea Patent No. 10-1654737 Korean Patent No. 10-1346244 Korean Patent No. 10-1433188

One aspect of the present invention is to provide a health functional food composition for prevention and improvement of hangover, which comprises extracts of Bacillus thuringiensis, Bacillus thuringiensis, Cinnamon, cinnamon and Staphylococcus aureus.

Another aspect is to provide a pharmaceutical composition for preventing or treating an alcoholic fatty liver comprising the above extract as an active ingredient.

One aspect of the present invention is to provide a health functional food composition for prevention and improvement of hangover, which comprises extracts of Bacillus thuringiensis, Bacillus thuringiensis, Cinnamon, cinnamon and Staphylococcus aureus.

The term " health functional food " as used in the present invention means any food or beverage manufactured and processed in the form of liquid, tablet, capsule, powder, granule, ring or the like using raw materials or components having useful functions in the human body. The health functional food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients that are conventionally added in the art. &Quot; Function " means that the structure and function of the human body are designed to exhibit useful physiological effects such as bio-defense, regulation of biorhythm, prevention of disease, improvement and recovery. The health functional foods of the present invention are useful for the treatment of hangover symptoms associated with acute and chronic alcohol consumption, for example, fatigue, general boredom, abdominal bloating, vomiting, heartburn and decreased cognitive function and other alcohol- For example, it can perform functions related to prevention, improvement, or recovery such as alcoholic liver disease, hyperglycemia, memory loss, reduction in concentration, and decrease in sensitivity.

The formulation of the health functional food can be manufactured without restriction as long as it is a formulation recognized as food and beverage. For example, dairy products, soups, drinks such as beverages for preventing and improving hangovers, tablets, flour, rings, meat, sausages, bread, chocolates, candies, snacks, confectionery, pizza, ramen and other noodles, gums and ice cream , A tea, a drink, an alcoholic beverage, and a vitamin complex, and may be added to juice, tea, jelly, and juice prepared from the extract of the present invention as a main ingredient.

The extract may be included in a health functional food in an amount effective to prevent and inhibit hangover. The effective amount may be appropriately selected depending on the cell or individual selected by a person skilled in the art. As used herein, the term " effective amount " or " as an active ingredient " refers to an amount of a composition sufficient to elicit a biological or medical response of an individual, relieve symptoms, prevent disease, Quot; This may be determined according to various factors including the kind of disease, the severity of the subject, the activity of the administered substance, sensitivity to the administered substance, administration time, administration route and rate of excretion, duration of treatment and concomitant drug. As an example, the effective amount may be in the range of 0.1 mg to 1,000 mg, such as 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 25 mg, 10 mg to 1000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg.

The term " hangover " or " alcohol-induced hangover ", as used herein, refers to alcohol and its metabolism such as side effects after ingestion of alcohol, such as fatigue, general boredom, abdominal bloating, vomiting, heartburn, Which is a complex clinical symptom induced by the product aldehyde.

The term " prophylactic " as used in the present invention means any action that inhibits the occurrence of a hangover or delays progression after administration of the composition of the present invention. The term " improvement " as used in the present invention means all phenomena in which the hangover phenomenon is improved by administration of the composition of the present invention. The term " administering " as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.

"칡" contained in the composition according to the present invention is a perennial plant, and its scientific name is Pueraria lobata Ohwi , which means a plant of soybean which survives most of the stem without freezing in winter. The stem of 칡 is classified as a tree because it grows thicker every year and forms a thick stem. In the present invention, " root " may be an extract extracted from the stem, root, or a part thereof, or a material derived therefrom of the perennial plant. 칡 grows in the shade of the foot of the mountain. It grows well in a place where the moisture and the ground are moderate. The stem extends more than 20m in length. It grows well on the sea, which is strong in the cold but salty. It has been used as a health food since it has been used as a wild plant for a long time. In one room, the root is used as a medicinal substance called 葛根, and it has effects such as sweating and fever. It contains daidzein, daidzin, puerarin, etc. as its main components. It also contains triterpenoid compounds, such as soyasapogenol A and kuzusapogenol B, which are known to induce sedation, hypothermia, and relaxation of blood vessels.

"Byeongryong" contained in the composition according to the present invention may mean a medicament prepared by scarring of Poria cocos Wolf with parasitized parasitic roots of pine root, and removing the outer layer. In the present invention, " bamboo shoot " may be an extract extracted from the sclerotia or a part thereof, or a material derived therefrom. In China and Japan, terms like Korea are used. In the old literature, it is said that it is called "Bongryeong" and "Bokshin." It is considered that the spiritual energy of the pine tree penetrates into the earth and is gathered together. There is also a record of being defeated. This medicine is almost odorless, slightly mucous, flavorful and plain, and the quality is flat without being shifted to one side. Byeongryeo can not see the urine, swelling of the abdomen and whole body, seaweed, vomiting, diarrhea when there is, and forgetfulness caused by nervousness, oil well, and also used for heart swelling. Baekbokgyeong is white, and red is Baekbokryeong. Polysaccharide digestion, which is a substance in which glucose is linked in chains, accounts for about 93%. In addition, pargumic acid, everricosan. It contains polypotenic acid A, C triterpenoid, elgosterol, lecithin, adenine, choline and the like. Herbal medicines have been used for kidney disease, cystitis, and urethritis due to the effects of tonic, diuretic, sedative and so on.

The "sine" included in the composition according to the present invention may refer to the fruit of Amanum villosum LOUR. Which is generally a perennial herbaceous plant belonging to the ginger family. In the present invention, the term " sign " may be an extract extracted from fruit or a part of the perennial herbaceous plant chrysanthemum or its inborn plants, or a material derived therefrom. The origin of Yangchun is Vietnam, Thailand and Cambodia. D-camphor, d-boneol, Bornyl acetate, Linalol, and Linalol are the main effective components of the active ingredients of the sine. · Neroldal and so on. In one room, the taste of medicine is slightly strong and warm. Pharmacotherapy is mainly reported in people who complain of digestive system and have complaints or have appetite.

&Quot; Cinnamon " included in the composition according to the present invention is Cinnamomum cassia Presl (camphor and Lauraceae)), some of which are removed from the juniper, materials derived therefrom, or extracts derived therefrom. Broiler chickens may have different origins and different qualities depending on the area. In particular, Vietnam has divided broilers into five classes. In addition, because broiler chickens have different picking and processing methods, the product standard is relatively comparable in terms of varieties, such as relationships, relationship, government, many. In the oriental medicine, he said, "Because he sees the kidney (kidney) well, he uses it as a medicine to treat the disease caused by the five bowel movements. The quality is hot and the taste is sweet and sweet. It has the efficacy of helping the sheep, defeating the Han, passing through the pores, and shrinking. When her stomachs are cold and her arms and legs are cold, they are used for low back pain, paucity, frequent urination, complete cold headache, cold headache,

The "stalks" contained in the composition according to the present invention include stalks of Lonicera japonica Thunberg, semi-evergreen broad-leaved shrubs, materials derived therefrom, or extracts extracted therefrom. In other terms, it is also referred to as a living thing. The stalks are cylindric in shape, and there are traces of cuts and branches on the nodes. Outer surface is reddish with vertical pattern and fine hairs. The young branches are densely hairy, the skin is easily broken off, and the grayish white neck is visible and fibrous. It is better to have thick branches. In one room, this medicine has little odor, taste is sweet and quality is cold. It is used for fever, detoxification, elimination of the whole body, sweating, arthritis of the extremities, skin itching, boils, infectious hepatitis, muscular pain, and arthritis. Pharmacological action was reported to release small intestinal seizures and inhibit staphylococci.

The " dermis " which may further be included in the composition according to the present invention may be an extract extracted from the skin of the mandarin, the skin of the diseased, the skin of the mandarin orange, the skin of the same plant or a part thereof, or a material derived therefrom. The dermis may mean the mature peri of the citrus unshiu Markovich or the relative plant. In Japan, Citrus unshiu Markovich (Citrus reticulata Blanco: 柑) is used as a dermis and Citrus tachibana (Makino Tanaka: 橘 柑) is used as a citrus peel. Citrus reticulata Blanco : 柑) and its cultivar varieties as dermis. In one room, the dermis releases loose bundles and strengthens the function of the spleen, treating only the abdominal cavity, trimming, vomiting, nausea, indigestion, sluggishness, lingering symptoms and dilated stools. Seawater, sputum and diuretic action. It has been reported that the longer the drug is, the more effective it is with the reading, magpie, dandelion, The dermis contains D-limonene, linalol, linalylacetate, and contains the flavonoids hesperidin, naringin, porcirin and nobiletin. The dermis has been reported to exhibit antiallergic activity, sedative effect, antiviral activity, improved serotonin action, and inhibiting liver damage.

&Quot; Gujuga nuts " which may be further included in the composition according to the present invention is the fruit of what is commonly called Lycium chinense Miller in Korea, the fruit of the same genus, the part of it, the material derived therefrom, Lt; / RTI > From ancient times, there is a record of using ginger fruit as a material. China uses Lycium barbarum L. and Japan uses Lycium chinense Miller and Lycium barbarum L. Ningxia japonica. In one room, there is little smell, it is astringent, and the weakness is slightly sweet and cold. It is dizzy and has no strength in the back and knees, and is used when a man is fat and can not conceive. When the blood is weak, the face is yellow and the hair is whitish, it is effective when it does not sleep. It is also used for long seawater due to fragility. Non-specific immune enhancement, hematopoiesis, cholesterol lowering, anti-hepatic action, blood pressure lowering, blood glucose lowering, growth promotion, and anti-cancer activity have been reported as pharmacological actions.

&Quot; Licorice " which may further be included in the composition according to the present invention may be selected from the group consisting of licorice (Glycyrrhiza uralensis Fischer), European licorice (Glycyrrhiza glabra L.) and other roots and stem parts of peat, , Materials derived therefrom, or extracts extracted therefrom. In China, dried roots of licorice (Glycyrrhiza uralensis Fischer: licorice), licorice (Glycyrrhiza glabra L .: light 果 licorice), licorice (Glycyrrhiza inflata Batal: 果 甘草) are used. In one room, licorice smells unusual and flavor is sweet. It has been reported that the harmful effects of all medicines are made harmonious, the medicinal effects are better, the control of the blood circulation and fraud, the communication of all blood vessels, and the strengthening of muscles and bones are reported. The present invention relates to a flavor enhancer which contains triterpenoid saponins glycyrrhizin and glycyrrhetinic acid and contains flavonoids such as liquiritigenin, isoliquiritigenin, liquiritin, Neoliquiritin and the like and isoflavonoids such as glycestrone, glycyrol, isoglycyrol, licoridine and the like. Known pharmacological activities include anti-inflammatory, anti-ulcerative, anti-inflammatory, antipyretic analgesic and estrogenic activities.

The " filament " which may additionally be included in the composition according to the invention is the Poncirus trifoliata Rafin), in particular, dried, less-ripe tangerine fruit, materials derived therefrom or extracts derived therefrom. Tangerine trees are planted in the central part of Korea and in other regions in the greenhouse. It dries off the less ripe fruit that has fallen to pieces. Use as it is or bran or honey as auxiliary material. In one room, it acts on the nerves (脾 经), the stomach (胃 经). Spread the molten machine, help digestion, and remove the phlegm. In pharmacological experiments, uterine contraction, gastrointestinal hyperactivity, hypertension, hypertension, diuretic effects were found. It can be used for dietary habits, digestive disorders, abdominal distension, constipation, dysentery, uterine drainage, gastrointestinal tract,

"Culprit", which may be further included in the composition according to the present invention, is one of the medicines used in Korea, China and Japan, and includes Senna tora, Cassia obtusifolia L. seeds, Materials or extracts derived therefrom. In the autumn, after the seed is dried and dried by the next stem, the seed is collected and collected. In one room, this medicine has a peculiar smell and taste, and the weakness is sweet, weaving, weaving and a little cold. It is used for night blindness and it treats the symptom that the eyes are congested, swollen and sick, the tear flows, and it is effective for the constipation which heat is piled up in the large intestine. It also reduces blood pressure and helps prevent arteriosclerosis. Pharmacological effects of blood pressure drop, diuretic, transmission, contraction of the uterus, skin fungi suppression, and cholesterol lowering function.

&Quot; Hovenia fruit, " which may be further included in compositions according to the present invention, is a hovenia tree of the Rhamnaceae dulis Thunb . ), Materials derived therefrom, or extracts derived therefrom. In China and Japan, it was not found in process medicines. This medicine has a bit of smell and the taste is good. In one room, the fruit of the hornbug (the earth) is burned due to fever, ejaculation, hiccups, vomiting, etc. It helps the diuretic and heals the upper liver by alcoholism. It is said that the stem skin of the hinoki tree is used as a globe neck to help the blood circulation and release the muscles. A liver protective effect was reported by pharmacological action.

The term " dry ginger " which may further be included in the composition according to the present invention may mean a dry root stem of ginger (Zingiber officinale Roscoe) and its inborn plants, and a part thereof, a material derived therefrom or an extract . In Japan, it is called dried ginger, and in Korea, it also uses health powder. In one room, the medicine has a peculiar smell and the medicine is spicy and hot. Chest and abdomen are cold, there is pain in the stomach, the stomach is not digested, and it is effective for vomiting and diarrhea. It is also used when the belly is cold and diarrhea occurs when the veins are weak, the limbs are cold, the seawater is generated in the cold wind, Pharmacological effect stimulates gastric secretion, activates intestinal peristalsis, promotes digestion, vomiting, and stimulates the heart to stimulate blood pressure and promote blood circulation. It has anti-inflammatory, analgesic and acaricidal action.

The composition ratio of the composition of the present invention is 2 ~ 10 weight ratio, 2 ~ 10 weight ratio, 2 ~ 8 weight ratio, 2 ~ 4 weight ratio, cinnamon weight, 2 ~ 4 weight ratio, Weight ratio. In another embodiment, the weight of the bean curd may be 5 to 7 weight ratio, the weight of the bean curd may be 4 to 5 weight, the sine may be 4 to 5 weight ratio, the cinnamon may be 3 to 4 weight ratio, and the sweet pot stalk may be 4 to 6 weight ratio. In another embodiment, the chewing gum may be 4 to 8 wt%, the chewing gum may be 3 to 6 wt%, the safflower may be 3 to 6 wt%, the cinnamon may be 2 to 4 wt%, and the phyllite may be 4 to 8 wt% .

The composition of the present invention may further comprise an extract of at least one of dermis, gum arabic, licorice, persimmon, cuttlefish, hornblende or dried ginger. In this case, the composition ratio is 2 to 8 weight ratio of the dermis, 1 to 5 weight ratio of the ginger fruit, 1 to 6 weight ratio of the licorice, 2 to 8 weight ratio of the licorice, 1 to 8 weight ratio of the saccharide, And the dried ginger may be contained at a weight ratio of 0.1 to 3 by weight. The saccharide is 4 to 5 weight ratio, the saccharide is 4 to 6 weight ratio, the saccharide fruit is 4 to 6 weight ratio, the saccharide fruit is 4 to 6 weight ratio, the saccharide is 4- to 6 weight ratio, And the dried ginger may be contained in a weight ratio of 0.5 to 1.5. In another embodiment of the present invention, the chewing gum is 4 to 8 weight ratio, the chewing gum is 3 to 6 weight ratio, the cod is 3 to 6 weight ratio, the dermis is 4 to 8 weight ratio, the gingko nut is 2 to 5 weight ratio, 3 to 6 weight ratio of cinnamon, 2 to 4 weight ratio of cinnamon, 3 to 6 weight ratio of flax seeds, 4 to 8 weight ratio of scutellum, 4 to 8 weight ratio of scutellum, 4 to 8 weight ratio of hinoki fruit, Dried ginger may be included at a weight ratio of 0.5 to 2. In another embodiment, the chewing gum is 5 to 7 weight ratio, the chewing gum is 4 to 5 weight ratio, the cod is 4 to 5 weight ratio, the dermis is 5 to 7 weight ratio, the gingko nut is 3 to 4 weight ratio, 4 to 5 weight ratios of cinnamon, 4 to 5 weight ratios of cinnamon, 4 to 5 weight ratios of flax seeds, 4 to 6 weight ratios of scutellum, 4 to 6 weight ratios, 4 to 6 weight ratios of hinoki wood, The ginger may be contained at a weight ratio of 0.5 to 1.5, but is not limited thereto.

Each of the materials constituting the composition of the present invention is not limited to the raw material itself or the pulverized product thereof but may be in various forms including a formulated (e.g., pulverized) work product, an extract of a raw material, Lt; / RTI >

In one embodiment, one or more of the materials that make up the composition of the present invention may be in the form of an extract or a juice obtained by treating the extraction solvent with the raw material. The extract has the meaning of being used as a crude extract in the art, but broadly includes fractions obtained by further fractionating the extract. As well as those obtained by further applying a purification process to the crude extract or fraction. For example, fractions obtained by passing the above extract through an ultrafiltration membrane having a constant molecular weight cut-off value, fractions separated by various chromatographies (prepared for separating by size, charge, hydrophobicity or affinity) And fractions obtained through various purification methods. The term " extract " and " fraction ", as used herein, are intended to encompass a broad range of preparations or preparations of the extracts and fractions of the extracts and fractions or of the dried or obtained extracts and fractions obtained by drying the concentrates, It is used in a sense.

The extract may be extracted with water, an alcohol such as a C1-C6 alcohol, for example, a C1-C4 alcohol or a mixture thereof as an extraction solvent. The C1-C6 alcohol may be methanol, ethanol, propanol, isopropanol, 1,3-propanediol, n-butanol, sec-butanol, isobutanol, tert-butanol, pentanol, hexanol or a mixture thereof. The solvent may be, for example, a mixture of water and an alcohol, that is, an aqueous solution of an alcohol. The alcohol concentration of the alcohol aqueous solution may be 1 to 99.5 (v / v)%, for example, 10 to 99.5 (v / v)%, 1 to 70 (v / 5 to 25 (v / v)%, 7 to 20 (v / v)%, 5 to 25 (v / v)%, or 10 to 20 (v / v)%. The alcohol aqueous solution may be an aqueous ethanol solution.

The materials may be in the form of finely divided particles which are chopped or ground. The materials may be dried or washed.

In one embodiment, the health functional food composition or the pharmaceutical composition provides a health functional food or a pharmaceutical composition comprising a pharmaceutically acceptable food-aid additive. The composition of the present invention may itself be formulated as a health functional food or beverage, or added to various foods and beverages.

In one embodiment, the compositions of the present invention may be formulated as conventional, including, but not limited to, functional foods, nutritional supplements, health foods, and food additives, Can be formulated as a kind of health functional food. As such pharmaceutically acceptable auxiliary additives, any carrier or additive known to be usable in the art for the preparation of the formulation to be prepared may be used.

In another embodiment, the present invention provides a beverage containing the composition for preventing and improving hangover. Such beverages or drinks can be consumed after drinking alone, or in addition to a high alcohol content mainstream before drinking.

Among the health functional foods of the present invention, beverage-type formulations may contain flavorings or natural carbohydrates as additional components such as ordinary beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of the flavoring agent include, but are not limited to, natural sweetening agents such as tau Martin and stevia extract, or synthetic sweetening agents such as saccharin and aspartame. The ratio of the natural carbohydrate is generally 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention. In addition, the health functional beverage of the present invention can be used as a nutritive, a vitamin, a mineral (electrolyte), a flavor such as a synthetic flavor and a natural flavor, a colorant and an enhancer, a pectic acid and its salt, an alginic acid and its salt, Colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. These components can be used independently or in combination, and the proportion of the additive is generally selected in the range of 0 to about 20 parts by weight of the composition of the present invention.

At this time, the amount of the extract in the food or beverage can be appropriately determined according to its use purpose (prevention, health, or therapeutic treatment, etc.). In general, in the case of a health functional food composition, it may be added in an amount of 0.01 to 15 parts by weight, preferably 1 to 5 parts by weight, based on the total weight of the food, and 0.02 to 10 g, May be added in a proportion of 0.3 to 1 g. However, in the case of long-term ingestion for the purpose of controlling health, the amount may be below the stated range.

In another embodiment, the present invention may be a pharmaceutical composition for the prevention or treatment of alcoholic fatty liver disease, which comprises extracts of Lilium, Liliaceae, Cinnamomum, Cinnamon, and Rhizome as active ingredients.

The pharmaceutical compositions of the present invention may be formulated into conventional pharmaceutical dosage forms known in the art. The formulations may be formulated and administered in any formulation, including, but not limited to, oral, injectable, suppository, transdermal, and intranasal formulations. In one embodiment, unit dosage forms of the present invention are in the form of tablets, pills, capsules, sachets, powders, granules, solutions, suspensions, emulsions, syrups, elixirs, extracts or aerosols, For oral administration.

The unit dosage form may further comprise a pharmaceutically or physiologically acceptable carrier conventionally used in accordance with the formulation. The carrier may be at least one selected from excipients, diluents, fillers, extenders, binders, lubricants, wetting agents, disintegrants, stabilizers, preservatives and surfactants. Examples of the carrier include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, But are not limited to, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In one embodiment, the compositions of the present invention are solid formulations for oral administration and may be in the form of tablets, pills, powders, granules, capsules, sachets, and the like. Such solid formulations may, in conjunction with the compositions of the present invention, comprise one or more pharmaceutical or physiologically acceptable excipients such as diluents, lubricants, binders, disintegrants, sweeteners, stabilizers, or preservatives. Specific examples of the diluent include lactose, corn starch, gelatin, calcium carbonate, soybean oil, microcrystalline cellulose, or mannitol, magnesium stearate or talc as a lubricant, polyvinylpyrrolidone or hydroxypropyl cellulose as a binder have. Examples of the disintegrant include carboxymethylcellulose calcium, sodium starch glycolate, polacrilin potassium, or crospovidone; examples of sweeteners include white sugar, fructose, sorbitol, or aspartame; stabilizers include sodium carboxymethylcellulose, White pigments, or xanthan gum; as an antiseptic, methyl paraoxybenzoate, propyl p-hydroxybenzoate, or potassium sorbate may be used.

In another embodiment, the pharmaceutical composition of the present invention may be in the form of an oral liquid formulation, such as a suspension, solution, emulsion, syrup, elixir, and the like. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included. In addition to the above components, natural additives such as natural flavors such as plum flavor, lemon flavor, pineapple flavor and herb flavor, natural fruit juice, natural coloring matters such as chlorophyllin and flavonoid, fructose, honey, sugar alcohol, sugar Or acidic agents such as citric acid and sodium citrate may be mixed and used.

In another embodiment, the pharmaceutical composition of the present invention may be formulated into a parenteral dosage form, including sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, suppositories, and the like. In the case of injections, it may be prepared by using physiologically compatible buffers such as, but not limited to, Hank's solution, Ringer's solution, and physiological saline buffer. As non-aqueous solvents and suspensions, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like, as well as stabilizers and dispersants. The compositions of the present invention may also be prepared in powder form and reconstituted by a suitable carrier, such as sterile water, prior to use. When formulated as a suppository, witepsol, macrogol, tween 61, cacao paper, laurin, and glycerogelatin may be used as the base, but the present invention is not limited thereto. Or may be formulated into a patch-type preparation.

A unit dosage formulation comprising a pharmaceutical composition of the present invention may be administered in a variety of routes to mammals such as humans, monkeys, horses, cows, pigs, goats, rabbits or rats. All modes of administration may be expected, for example, oral, intraarticular, intravenous, muscular, subcutaneous, transdermal, rectal, nasal, intracerebral, or intracerebroventricular administration. The compositions may also employ other methods known in the art, such as those described in the latest edition of Remington ' s Pharmaceutical Sciences. In a most preferred embodiment, the composition can be formulated in unit dosage form for oral administration.

The dosage of the pharmaceutical composition according to the present invention is preferably 3 g to 20 g, but is not limited thereto. The actual dosage may vary depending on factors such as age, body weight, severity of symptoms, alcohol intake, frequency, metabolic rate, responsiveness of the patient, and drug side factors such as dosage form, route of administration, And may be suitably selected by those skilled in the art. In a preferred embodiment, the daily dose of the composition of the present invention may be optionally administered in portions, such that the dose is 3 g to 20 g, preferably 5 g to 15 g, more preferably about 10 g, on an adult basis .

In another aspect, the present invention provides a method for producing a water-in-oil emulsion, comprising: providing 4 to 8 weight ratios, 3 to 6 weight ratios, 3 to 6 weight ratios, 2 to 4 weight ratios of cinnamon, 4 to 8 weight ratios, Extracting the extract using an extraction solvent; Separating the liquid from the extract; Treating the liquid with an enzyme to decompose the enzyme; And a step of inactivating the enzyme of the liquid and then filtering the solution. In the step of providing the ginseng and the like, it is possible to extract by further providing a dermis, a ginseng, a licorice, a ginseng, an alder, a cutter, a hinoki fruit or a dried ginger.

In one embodiment, the step of extracting the extract using the above-mentioned extraction solvent may further comprise the step of extracting the extracts from the above-mentioned extracts, bamboo shoots, cinnamon, cinnamon, alfalfa stalks, dermis, ganoderma lucidum, licorice, The extraction solvent may be added in an amount of from 3 to 10 (vol / g) times, for example, from 3 to 7 (vol / g) times, from 3 to 5 (vol / 10 fold. ≪ / RTI > For example, for 1 kg of the above-mentioned material, 1 kg of the above-mentioned material, the bamboo, the cinnamon, the cinnamon, the stalks and the dermis, the gum, the licorice, the licorice, And adding 3 to 10 L of a solvent.

The extraction may be performed by warmed liquid extraction, pressurized liquid extraction (PLE), microwave assisted extraction (MAE), subcritical extraction (SE), or a combination thereof . The subcritical extraction may be subcritical water extraction (SWE). Sub-critical water extraction is also referred to as superheated water extraction or pressurized hot water extraction (PHWE). The warmed liquid extraction may be a reflux extraction.

The extraction may be carried out at a temperature of from 4 ° C to 100 ° C, for example from 10 ° C to 100 ° C, from 20 ° C to 100 ° C, from 50 ° C to 100 ° C, from 10 ° C to 95 ° C, Deg.] C to 95 [deg.] C, 70 [deg.] C to 95 [deg.] C, 80 to 100 [deg.] C, 80 to 95 [deg.] C, or 90 to 100 [ The extraction time may vary depending on the temperature selected and may range from 1 hour to 2 months, such as 1 hour to 1 month, 1 hour to 15 days, 1 hour to 10 days, 1 hour to 5 days, 1 hour to 3 days 4 hours to 2 days, 1 hour to 1 day, 4 hours to 1 month, 4 hours to 15 days, 4 hours to 10 days, 4 hours to 5 days, 4 hours to 3 days, 4 hours to 2 days, 4 4 hours to 1 day, 4 hours to 1 day, 4 hours to 15 days, 4 hours to 10 days, 4 hours to 5 days, 4 hours to 2 days, or 4 hours to 12 hours.

The step of separating the liquid from the extract may include separating the liquid by a known method such as filtering the plant residue and the extract. The extraction may also include removing the solvent from the resulting extract by known methods, such as concentration under reduced pressure. In one embodiment, the liquid can be separated in the same manner as a filter press.

The enzyme in the step of enzymatically digesting the liquid by enzymatic digestion may be a mixture of α-1,4-galacturonic acid (α-1,4-galacturonic acid) such as pectinic acid, pectin, pectic acid, 1,4-galacturonic acid) bond, and specific examples thereof include pectinase, polygalacturonase, polymethylgalactronase, . The pectinase is an endo-type pectinase which generates oligosaccharides by decomposing randomly from the inside of the [alpha] -1,4 linkages in the action type of the enzyme, and an exoproteinase (pectinase) The former is classified into liquefied, the latter belongs to glycated pectin hydrolase. In addition, specificity to the substrate may also be attributed to pectin having a high methyl esterification or low-pectic acid, so that the enzyme acting on the former is called polymethylgalacturonase and the latter enzyme is called polygalacturonase polygalacturonase).

The step of inactivating and filtering the enzyme of the liquid may be by treating the enzyme digesting solution, heating and then filtering. In one embodiment, the step of filtering after the enzyme inactivation may further comprise the step of concentrating. After the concentrating step, sterilization may be further included. After the sterilizing step, it may further include a specification inspection and packaging step. After the sterilizing step, it may further comprise adding to the health improving food.

A health functional food composition for prevention and improvement of hangover according to one aspect and a pharmaceutical composition for prevention or treatment of alcoholic fatty liver can be safely used using a plant extract, easy to take and can be stored for a long period of time, Administration of the present invention accelerates the metabolism of alcohol in vivo to rapidly lower the blood alcohol concentration, thereby exhibiting a hangover resolution effect or prevention or treatment effect of alcoholic liver disease.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a method for producing a health functional food composition (formulation: beverage) for prevention and improvement of hangover according to the present invention.
Fig. 2 shows the results of the usual hangover symptoms among 13 male and 13 female.
FIG. 3 is a graph showing symptoms of experiencing hangover improvement efficacy as a result of administering the composition according to the present invention to 13 male and 13 female subjects.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1. Prevention and improvement of hangover Preparation of health functional food composition

First, the materials shown in Table 1 were prepared in each dry state. The source of each material used in this embodiment, the detailed use site, etc. are as shown in Table 1 below.

Material name source part Weight (g) 칡 China Root 6.6 Boryeong China Sclerotia 4.95 sign Myanmar seed 4.4 dermis China Fruit skin 5.5 Gujarat tree China Fruit 3.85 licorice Uzbek Root 4.13 cinnamon Indonesia Bark 3.3 House China Fruit 4.67 Indwelling China stem 5.5 Loser India Fruit 5.5 Hovenia fruit China Fruit 5.5 Gun ginger China Root 1.1

1.1. Health functional food composition 1: 칡, bokyong, sin, cinnamon and Stalactite  Composition comprising extract

Compositions of the present invention were prepared in the same manner as the preparation method shown in Fig. 1. Specifically, each material was prepared according to the weights shown in Table 1 and transferred to an extraction tank. Purified water was prepared at 10 times the volume of the raw material and reflux-extracted at 95 ° C for 4 hours. Then, the liquid was separated using a filter press (Korea Filter Co., Ltd., 4 μm pore). After digestion with pectinase (1.3% of the raw material, Sigma Aldrich Korea), the enzyme was digested (50 ° C, 12 hours). Then, the enzyme solution was heated at 90 DEG C for 10 minutes to inactivate the enzyme, and the extract was filtered to obtain an extract. The extract was concentrated to 60-80 Brix and sterilized (95 DEG C, 40 minutes, twice). Thereby completing the beverage formulation.

1.2. Health functional food composition 2: composition further comprising extract of additional material

The mixed extracts of 칡, Byeongryeong, Sine, Dermis, Gugija, Lycopersicum, Cinnamon, Ginseng, Stalks, Cucumber, Hovenia dulcis, and Gunghi were prepared as follows. Each material was prepared according to the weights shown in Table 1 and transferred to an extraction tank. Purified water was prepared at 10 times the volume of the raw material and reflux-extracted at 95 캜 for 4 hours. Then, the liquid was separated using a filter press (Korea Filter Co., Ltd., 4 μm pore). After digestion with pectinase (1.3% of the raw material, Sigma Aldrich Korea), the enzyme was digested (50 ° C, 12 hours). Then, the enzyme solution was heated at 90 DEG C for 10 minutes to inactivate the enzyme, and the extract was filtered to obtain an extract. The extract was concentrated to 60-80 Brix and sterilized (95 DEG C, 40 minutes, twice).

Example  2. Preparation of Beverage Formulation in Health Functional Foods

A beverage containing the composition of Example 1.2. Above was prepared. The detailed contents are shown in Table 2 below. All materials were weighed and placed in a preparation tank, followed by stirring at 85 ° C for 30 minutes.

material content Capacity Composition 5% 5g honey 8% 8g Purified water 87% 87g Sum 100% 100ml

2.1. 40Brix  Manufacturing of beverage formulations

The beverage thus prepared was concentrated to 40 Brix to complete the beverage.

2.2. 75Brix  Manufacturing of beverage formulations

It was also concentrated to 75 Brix to complete beverages of different concentrations.

Example  3. Comparative example  Manufacturing and preparation

3.1. Comparative Example  1: Preparation of mixed extract 1

The mixed extract 1 (55 Brix) was prepared in the same manner as in Example 1.1, except that the components of the mixed extract were changed as shown in Table 3 below.

Herbal medicine  persons  Weight (g) 칡 12.1 Boryeong 8.8 sign 8.8 Mixed Extract 1

3.2. Comparative Example  2: Preparation of mixed extract 2

The mixed extract 2 (55 Brix) was prepared in the same manner as in Example 1.1, except that the ingredients of the mixed extract were as shown in Table 4 below.

Herbal medicine  persons  Weight (g) sign 8.5 Stalactite 10.5 Mixed extract 2

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Experimental Example  1: Prevention and improvement effect of extract hangover

1.1. Preparation of animal experiments

Thirty male 6-week-old ICR (Institute of Cancer Research) mice were admitted to the laboratory for 7 days at a temperature of 22 ± 3 ° C, relative humidity of 50 ± 20% and illumination of 150-300 Lux, Lt; / RTI > During the adaptation period, reverse osmosis ultrapure water using R / O system was used as drinking water, and Purina as feed, and the weight deviation range between test animals was within ± 20% Respectively.

The test animals were divided into 10 groups of 3 animals per group so that the average weight of each group was evenly distributed as much as possible on the day of administration. For the identification of individuals during the test, the tail marking method was used.

1.2. Statistical processing and analysis

The statistical significance of the results of body weight and serum analysis (ethanol, acetaldehyde, CYP2E1) was analyzed by student's t-test for variability from the control value and statistically significant when p value <0.05 Respectively. Specifically, when the p value was less than 0.05, the value was marked with ^* , and when it was less than 0.01, the value was marked with † to judge the difference in significance.

1.3. Analysis of improvement effect of hangover

As the test substance, there were used the compound of Example 1.2, which was prepared in Example 1. (55 Brix, 210, 420 mg / kg B.W.), Example 1.1. (40 Brix, 420 mg / kg B.W.) was used. As a control, 200 μl of saline 0.9%, Comparative Examples 1 to 3, Comparative Hangover Drink 1 (Condition, CJ Healthcare Co., Ltd., 37.4 Brix depressurized concentrate, 400 mg / kg BW) and Comparative Hangover Drink 2 Grammy, 39.2 Brix depressurized concentrate, 400 mg / kg BW) was used. The known materials of Comparative Hangover Drink 1 Condition contain 1.3% of purified water, liquid fructose, microbial fermentation extract (Korean rice, rice gruel, rice bran extract, soybean peptide), rice bran, taurine, , Yeast extract powder (containing 5% glutathione), spot extract powder, Hwanggi lotus extract powder, anhydrous citric acid, DL-malic acid, dextrin, alanine, sodium citrate, nicotinamide, complex gold extract, Honeycomb flavor, honey flavor) 2.4%, and comparative hangover beverage 2 flavor 808. The known ingredients of the 808 include purified water, daffodil concentrate [Domestic: Auckland, Jujube, Ginger) mixed concentrate (Made in China: Honey)] and honey.

Ethanol (Sigma Aldrich Korea) was diluted in distilled water to prepare a 60% ethanol solution. The preparation was carried out immediately before administration, and no separate analysis such as stability or purity was performed.

Fasting was started 18 hours before the administration of the test substance to eliminate differences in alcohol absorption and degradation by diet. Three mice per group were used and each group was treated as shown in Table 6 below.

group Dose Group 1: negative control group Saline 0.9% 200 μl + Saline 0.9% 400 μl / kg B.W. Group 2: ethanol treated group 60% Ethanol 200 μl + Saline 0.9% 400 μl / kg B.W. Group 3: Ethanol + Example 1.1. Treated group 60% Ethanol 200 ul + Example 1.1. 420 mg / kg Group 4: Ethanol + Example 1.2. Low dose treatment group 60% Ethanol 200 ul + Example 1.2. 210 mg / kg Group 5: Ethanol + Example 1.2. High dose treatment group 60% Ethanol 200 ul + Example 1.2. 420 mg / kg Group 6: Ethanol + Comparative Example 1 Treatment group 60% Ethanol 200 ul + Comparative Example 1 420 mg / kg Group 7: Ethanol + Comparative Example 2 Treatment group 60% Ethanol 200 ul + Comparative Example 2 420 mg / kg Group 8: ethanol + comparative hangover beverage 1 treated group 60% Ethanol 200 μl + Hooded Beverage 1 400 mg / kg Group 9: ethanol + comparative hangover drink 2 treated group 60% ethanol 200 μl + comparative hangover beverage 2 400 mg / kg

Specifically, the experimental material was orally administered to each experimental animal group 30 minutes before the ethanol intake. Then, 200 μl of 60% ethanol was orally administered. Two hours after ethanol ingestion, a 5: 1 mixture of anesthetics (zoletil, Rumpun) was diluted 1/7 and administered intraperitoneally at 6 mg / kg B.W.), and then subjected to cardiac collection. Whole blood was centrifuged (4 ° C, 10,000 rpm, 15 min) and the serum was separated and stored at -80 ° C before use. Serum acetaldehyde was measured by ELISA kit (Acetaldehyde kit, Roche, Switzerland) using separate sera. Serum ethanol was measured by ELISA kit (Ethanol kit, Roche, Switzerland) using separate serum, and CYP2E1 was also measured by ELISA kit (Mouse Cytochrome p450 2E1 (CYP2E1) ELISA kit, China) using the separated serum.

1.4. Comparison of Ethanol Concentration in Plasma

The concentration of ethanol in the plasma is shown in Table 7.

group Plasma ethanol concentration (g / l) Group 1: negative control group 1.15 Group 2: ethanol treated group 5.20 Group 3: Ethanol + Example 1.1. Treated group 2.37 Group 4: Ethanol + Example 1.2. Low dose treatment group 1.30 * Group 5: Ethanol + Example 1.2. High dose treatment group 0.38 * Group 6: Ethanol + Comparative Example 1 Treatment group 1.96 Group 7: Ethanol + Comparative Example 2 Treatment group 2.18 Group 8: ethanol + comparative hangover beverage 1 treated group 2.97 Group 9: ethanol + comparative hangover drink 2 treated group 2.08

As shown in Table 7 above, groups 4 and 5, which are health functional food compositions according to the present invention, showed significantly lower ethanol concentration. In particular, Group 4 showed a similar effect compared to the negative control group. Compared with Comparative Examples 1 and 2 prepared by selecting two or three components out of the five components of the present invention, groups 3, 4 and 5 containing all five components exhibited remarkably superior ethanol decomposition effects. Ethanol concentration in plasma was similar or statistically significant compared with comparable hangover beverages 1 and 2 on the market. Therefore, it was confirmed that the composition of the present invention improves the ethanol metabolism rate in the body of ethanol.

1.5. Comparison of Acetaldehyde Concentrations in Plasma

The concentration of acetaldehyde in the plasma is shown in Table 8.

group Plasma acetaldehyde concentration (mg / l) Group 1: negative control group 74.67 Group 2: ethanol treated group 297.49 Group 3: Ethanol + Example 1.1. Treated group 80.05 Group 4: Ethanol + Example 1.2. Low dose treatment group 134.41 Group 5: Ethanol + Example 1.2. High dose treatment group 178.02 Group 6: Ethanol + Comparative Example 1 Treatment group 200.70 Group 7: Ethanol + Comparative Example 2 Treatment group 220.56 Group 8: ethanol + comparative hangover beverage 1 treated group 126.64 Group 9: ethanol + comparative hangover drink 2 treated group 174.432

As shown in Table 8 above, the concentration of acetaldehyde in blood was higher than that of Example 1.2 according to the present invention. Low dose, high dose group, and Example 1.1. And the tendency to show lower tendency in the intake group than in the positive control group. Thus, it was confirmed that the composition of the present invention improves the rate of acetaldehyde metabolism in the body when consumed with ethanol.

1.6. Plasma CYP2E1  Concentration comparison

Plasma CYP2E1 concentrations are shown in Table 9.

group Serum CYP2E1 concentration (pg / ml) Group 1: negative control group 36.5 Group 2: ethanol treated group 50.0 Group 3: Ethanol + Example 1.1. Treated group 49.6 Group 4: Ethanol + Example 1.2. Low dose treatment group 18.1 * Group 5: Ethanol + Example 1.2. High dose treatment group 19.5 * Group 6: Ethanol + Comparative Example 1 Treatment group 19.6 Group 7: Ethanol + Comparative Example 2 Treatment group 20.2 Group 8: ethanol + comparative hangover beverage 1 treated group 30.5 * Group 9: ethanol + comparative hangover drink 2 treated group 49.8

As shown in Table 9, in Group 4 and Group 5, CYP2E1 showed a significantly lower concentration than the positive control group. Therefore, it was confirmed that the composition of the present invention inhibits the expression of CYP2E1, which is associated with the induction of alcoholic liver damage.

Experimental Example  2. Human clinical trials

2.1. Experimental Method

We conducted a questionnaire survey on 13 men and 13 women. Each age group was 20 to 2, 30 to 13, 40 to 9, 50 to 1, and 60 to 1. The average amount of alcohol consumed was one bottle of shochu, and the average intake of alcohol during the experiment was 1.23 bottles. The intake period of the test substances was 18 before drinking, 4 after drinking, and 4 unrecognized.

2.2. Pre-hangover symptom investigation

As shown in FIG. 2, the usual symptom of hangover specific symptoms such as headache, nausea, vomiting, helplessness, insomnia, anorexia, excitement, excitement, fever and heartburn is 3 or 3, The symptoms were headache 20, anorexia 2, nausea 6, vomiting 6, sleeplessness, insomnia, 3 persons, 6 helplessness, 1 excitement, 1 other fever and 1 heartburn.

2.3. Prevention and improvement effects of hangover symptom

There were 23 patients who experienced the prevention and improvement of hangover symptoms and 3 patients who were not experienced. As a result of examining the number of experience efficacy of 23 experienced people, there were 10 people in 2 people and 13 people in 1 person. The results were as follows: 1) improvement of headache, 2) improvement of appetite, 1) improvement of nausea, 5) improvement of vomiting, 1) improvement of insomnia, 4) improvement of excitement, 2) 1 person, moderate ease, 1 late drinker, 1 drinker. These results are shown in Fig. On the other hand, there were 3 experienced side effects: 1 insomnia, 1 headache, 1 heartburn. The intention to purchase was 22 out of 26. As a result, human clinical trials confirmed that the composition according to the present invention had an effect of preventing and improving hangover.

Formulation example  One: Gelatin  Produce

Composition of Example 1.1 ............ 2 g

Bee honey ................................ 0.8 g

Concentrated concentrate ... 2.5 g

Xanthan gum .............................. 0.001 g

Taurine ................................ 0.1 g

Purified water .......................... 4.599 g

The above components were mixed according to a conventional food manufacturing method, and a gel was prepared. It can be used for the production of a health food composition according to a conventional method of manufacturing the food.

Formulation example  2: Manufacture of beverages

2.1. Manufacture of beverages for hangover

To 15 parts by weight of the composition of Example 1.1, 0.1 part by weight of vitamin C, 6 parts by weight of fructose, 4 parts by weight of white sugar, 0.1 part by weight of citric acid and 0.04 part by weight of sodium citrate were mixed and dissolved completely in metered water. After stirring and heating at 85 DEG C for about 1 hour, the solution thus obtained was filtered and sterilized in a sterilized container, followed by sealing sterilization and then refrigerated.

2.2. Manufacture of health drinks

15 parts by weight of the composition of Example 1.2, 0.5 parts by weight of liquid fructose, 2 parts by weight of oligosaccharide, 2 parts by weight of sugar, 0.5 part by weight of salt, and 75 parts by weight of water were uniformly blended and instantly sterilized, Health drinks were prepared by packaging in a small package.

Formulation example  3: Preparation of pharmaceutical preparations

3.1. Sanje  Produce

Composition of Example 1.1 10 g

Lactose ......................... 1 g

Talc ....................... 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

3.2. Preparation of capsules

Composition of Example 1.1 ... 500 mg

Lactose .......................... 250 mg

Corn starch ................... 250 mg

Talc ..........................., 6 mg

Magnesium stearate ............. 5 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Claims (5)

A health functional food composition for prevention and improvement of hangover, which comprises extracts of green tea, green tea, cinnamon, cinnamon, alfalfa, dandelion, ginger, licorice, persimmon, killer, hinoki fruit and dried ginger. The health functional food composition according to claim 1, wherein the extract is extracted using water, alcohol or a mixture thereof as an extraction solvent. The method according to claim 1, wherein the ginger is in a range of 4 to 8: 3 to 6: 3 to 6: 2 Wherein the weight ratio of the active ingredient to the active ingredient is from 4: 4 to 8: 4 to 8: 2 to 5: 3 to 6: 3 to 6: 4 to 8: 4 to 8: 0.5 to 2. delete A pharmaceutical composition for the prevention or treatment of alcoholic fatty liver disease, which comprises as an active ingredient an extract of bamboo shoots, bamboo shoots, cinnamon, cinnamon, dandelion, ganoderma lucidum, licorice root,

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