KR100747415B1 - Composition for improving mastitis comprising herb extracts - Google Patents

Abstract

A composition for improving mastitis comprising herb extracts is provided to alleviate mastitis accompanied with flare, hot flash and pain by controlling female hormone, improving immune response, and exhibiting antiinflammatory and antimicrobial effects. The composition for improving mastitis comprises 0.001-5 wt.% of the extracts of herbs including Trigonella foenum Graecum, Cimicifuga Racemosa, Punica Granatum, soy isoflavones, Linum Usitatissium(linseed) and honey which are prepared by extracting the herbs with a solvent selected from water, C1-C4 lower alcohol, ethylene, acetone, ether, hexane, chloroform, ethylacetate, DMF(N,N-dimethylformamide), DMSO(dimethylsulfoxide), 1,3-butyleneglycol aqueous solution, propylene glycol and a mixture thereof at 50-100 deg.C for 3-24 hours. The composition is formulated as soft lotion, nutrition lotion, nutrition cream, massage cream, essence or pack.

Description

Composition for improving mastitis comprising herb extracts

1 is a graph of cytotoxic effects of phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

Figure 2 is a graph of the cell immunity effect of Phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

Figure 3 is a photograph of the antimicrobial effect of pignegrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

Figure 4 is a graph of the hormonal regulatory effect of phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

Figure 5 is a change picture of the breast due to mammary gland development of fenugreek, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

Figure 6 is a thermographic improvement thermography of the breast of phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture.

The present invention relates to a cosmetic composition having a woman's breast tenderness-reducing effect, and more particularly, to fenugreek ( Trigonella Foenum - Graecum Extract, Horse Riding ( Cimicifuga Racemosa ) Extract, Pomegranate ( Punica) Granatum) extract, soy isoflavones (Soy isoflavones) extract, linseed (Linum Usitatissium (Linseed) relates to a cosmetic composition having a breast tenderness-reducing effect comprising an extract and a honey extract.

Women's breasts start to develop before menarche, and the female hormones estrogen and progesterone are closely related, which leads to controlled growth and a gradual change in life.

At puberty, the secretion of gonadotropins from the anterior pituitary gland increases estrogen secretion, which stimulates breast development and starts ovulation, which starts progesterone secretion, which leads to breast maturation. During this time, you will feel bruising and pain in your chest. Also, about a week before menstruation, the fluid is produced by the effects of the female hormones estrogen and progesterone. At this point, painful monguri occurs.

These breasts can be said to undergo full maturity with pregnancy. Women's breasts undergo pain and breast changes from the early stages of pregnancy, which means that the female hormones estrogen and pregesterone, including lactate producing prolactin (Prolactin) This is due to the action of and Oxytocin (Oxytocin).

Most women begin menopause around 50 years of age, the size of the mammary glands and ducts begin to shrink. In addition, because the fiber tissue that maintains the form of the breast becomes thinner and loses elasticity, the breast sags with age. During this period, the production of female hormones estrogen and progesterone is greatly reduced, irregular menstruation, and breast pain, lumps or papillary discharge may be observed.

Breast swelling is breast congestion that causes the breast to harden and swell for 1-2 days after the beginning of breastfeeding, often with pain and temporary temperature rise. It is a precursor of normal secretion of milk, caused by congestion of the veins and lymph glands, and not by overexpansion of the mammary gland. Breast congestion is due to the fact that the milk already made during pregnancy does not come out. This symptom is called breast meat. Breast fever due to breast congestion is common, duration of fever is 4-16 hours, body temperature rises 37.8 ~ 39 ℃. In addition, before menstruation, during pregnancy or menopausal women, changes in the development of the mammary gland under the influence of the female hormones estrogen and progesterone cause symptoms such as pain or fever.Inflammation due to external infection or mammary gland or vascular abnormalities This may be accompanied. Therefore, in order to improve and prevent breast congestion in women, internal immunity should be strengthened, and hormone secretion should be normalized to improve mammary gland development, to prevent inflammation, and to prevent secondary infections from the outside. It is important.

As part of a study to obtain a substance having excellent relaxation effect on women's breast meat from natural products, the present inventors selected natural extracts for breast meat relaxation from natural products that have been used as cosmetic raw materials. Equestrian, pomegranate, soy isoflavones, flaxseed or honey extracts have been found to have excellent hormone regulation, improved immunity, anti-inflammatory and antimicrobial effects. Furthermore, the mixtures of these extracts have better effects than the individual extracts and complete the present invention. It was.

Accordingly, an object of the present invention is to provide a novel cosmetic material having both hormone regulation, immunity improvement, anti-inflammatory, and antibacterial action, and which can be usefully used for relieving breastfeeding of women. In addition, the present invention is to provide a cosmetic composition comprising a novel cosmetic material having the above efficacy.

The present inventors selected 20 natural products having anti-inflammatory, immunity improvement, and hormonal control effects in order to select natural products effective for alleviating breast congestion (breast meat) in women. NF-kB promoter inhibitory ability by examining the extent of a review of anti-inflammatory herbal extracts, immunity improvement results penyu Greek seed extract (Trigonella Foenum- Graecum Seed Extract, Horse Riding Extract ( Cimicifuga Racemosa Root Extract, Punica Granatum Extract), soy isoflavones, linseed extract ( Linum Usitatissium ( Linseed ) Seed Extract) were found to show excellent anti-inflammatory, immune-improving effect. As a result of measuring the hormonal regulation effect of the natural extracts by progesterone receptor expression level, it was also confirmed that fenugreek, horse riding, pomegranate extract, soy isoflavin, flaxseed extract had excellent hormonal regulation effect.

Known features, efficacy, and effects of each of these components are as follows.

Phenegrig ( Trigonella Foenum-Graecum ) is a year-old plant belonging to the legume and grows up to 30 ~ 60cm. The leaves of three leaves are dark green, but the leaves are white, and the back is white and has three leaves. . Flowers bloom in June-August, one or two butterfly-shaped white flowers bloom in the axilla, and the fruit pods are bent like a new snout. There are about 10 seeds that are gathered like seeds of a defector, and when they are ripe, they become yellowish brown. There is a fragrance throughout the abandonment, but it has a particularly strong aroma to the seeds, and the seeds are sticky when wet. It is widely cultivated in India, China, Southeast Asia, France, Germany, Greece, Sudan, Morocco, Pakistan, Lebanon, and Argentina. This seed extract contains a large amount of 4-hydroxyisoleucine, one of the natural amino acids, which is known as a major indicator of physiological activity, and various papers have recently been published. Notable functions have been shown to be directly involved in the effects of insulin secretion and resistance and in the direct control of blood sugar control and ultimately excellent effect on type 2 diabetes. In addition to antidiabetic effects, Phenegrig extracts have antioxidant effects ("Effect of fenugreek seeds on blood lipid peroxidation and antioxidants in diabetic rats." Phytotherapy Research 13, 197-201, 1996) anti-inflammatory effects ("Trigonella foenum graecum (fenugreek) seed extract as an antineoplastic agent. "Phytotherapy Research 15, 257-259, 2001) and various biological functions for immunological enhancement effects (" Immunomodulatory effects of fenugreek extract in mice ". International Immunopharmacology 3 (2003) 257-265). It is one of the most likely materials to serve as the next generation of functional materials.Phenegrig Seed Extract is also a very safe material, according to Professor Ramesh at the National Food Research Institute of India in 1999. Single Dose 2 Results from Animal Experiments Using Albino Rats and Albino Mice on Bitter-Free Substances No toxic lesions occurred when g / kg to 5 g / kg of substance was administered, and subacute toxicity experiments were conducted with 90, 1, 5, and 10% concentrations of fenugreek seed extract solution for 90 days. Consecutive doses showed no effect on Daily Food Intake and Growth, demonstrating the high safety of the Phenygrig seed extract, and ancient literature has shown that this Phenugrig seed extract was originally a woman. It has been shown to have been used as a natural oral cosmetic that has been widely loved by women of ancient Egypt and Greece because of its effect on making their breasts bigger.It also has various effects such as anti-ulcer, hair loss prevention, boil treatment and endometritis treatment. Known.

Horseback Riding ( Cimicifuga Racemosa ) is distributed in Korea, northeastern Jung-gu, and Ussuri River, and grows in deep mountain forests. It is about 1m high and its root is thick and purplish black. Horse riding contains substances that act like estrogen in women. In addition, it has the same effect as anti-inflammatory and anti-convulsion and is a good tonic for women's womb immediately after delivery. Since 1950, many clinical data have been published that the standardized equestrian extract formulations are used for the treatment of menopausal symptoms, menstrual disorders, and premenstrual syndromes. As has been officially approved for the efficacy of the extract, horse riding is known to have an excellent effect related to gynecological diseases by controlling the estrogen effect.

Pomegranate ( Punica) Granatum ) is the fruit of Punica granatum, a shrub or joiner from Asia, and a few species from Scotra. This fruit belongs to the pomegranate family. Pomegranate is 5 ~ 7m long, has bright green lanceolate leaves about 75mm, and has reddish orange flowers of lobe growing at the end of branches. The calyx is tubular and has 5 to 7 round protrusions. The petals are lanceolate and inserted in the calyx protrusion. Fruits are similar to the size of large oranges and are soft, have skin-like skins, and vary in color from tan to red. Root and stem bark contain 0.3 ~ 0.5% of alkaloids such as Pelethierin, Isopeletierin, Methyl Isobelerin and Pseudopeletierin. Most of the alkaloids in the root bark (30-40% of the total base) are isofelethierin. The alkaloid content varies depending on the variety, part of the tree, and the seawater grown. It is usually 0.2 ~ 0.7% in the root bark and about 0.5% in the bark. The roots and stems also contain 22-25% tannins, which are glucosides of ellagic acid. Others include ursolic acid (fruit-0.6%, leaf-0.45%), beuric acid (bark-0.15%, leaf -0.2%), mannitol, and ecumol. The fruit peel contains tannins (28%), the fruit juice contains sugars (10-12%), and organic acids. Pomegranate is known to be a woman's fruit, the main effect of the pomegranate is found in the unique ingredients contained in the pomegranate, especially in the rich content of potassium, pectin, tannins in addition to sugars, amino acids, vitamins, acids The most important component of pomegranate is the estrogen-like hormone called estradiol, which is almost identical in structure to female hormone secreted by human body than any other plant on earth. The fact that it contains -18 mg has already been announced. Female pomegranate in pomegranate is more water soluble and similar to hormone secreted by human body compared to chemical and synthetic hormones, so it is easy for the receptors in the body to accept and consume the necessary amount. Afterwards, the remaining amount is likely to be safe because it is excreted and not accumulated in the body. It is found that eractan when hydrolyzed by eragtannin combined with tannin is hydrolyzed. It has been found to have strong anticancer activity, and is effective in antioxidant activity and acid secretion. It is known to be effective in the prevention of senile dementia and male prostatitis in relation to the function of hormones, and the effect of pomegranate in Chinese medicine is used as a medicine to protect the gods. Because the pomegranate flower is used for oral inflammation, such as stomatitis, laryngitis and tonsillitis, or the bark as an oral and intestinal disease, abdominal pain, repellent, and leaves to prevent vomiting or appetite. It is also known to have diarrhea, diarrhea, bleeding, and diarrhea, dysentery, oil wells, and cramps.

Soy Isoflavones are the most notable isoflavones among the various components of soy. Isoflavones are a type of phytochemical defect that is produced by plants to protect themselves from harmful factors in nature. The main isoflavones found in soybeans are genistein and dyedzein, both of which act as plant female hormones. Genistein is a phytoestrogen and is known to be effective in preventing osteoporosis and menopausal syndrome, as well as various cancers such as breast cancer, prostate cancer and lung cancer. Genistein is the most representative phytoestrogens. Genistein is a vegetable isoflavone that has a strong anticancer activity. In addition, the concentration of estrogen is reduced by about 30%, resulting in various menopausal symptoms.The menopausal symptoms such as hot flashes, sweating, nervousness, depression, sleep disorders, and hyperhidrosis are improved in postmenopausal women. It is known as a new concept of substance that can replace hormones. Genistein can relieve symptoms with hormone therapy, such as estrogen-progesterone. It is known to induce menstrual cycle changes to prevent PMS and alleviate symptoms when isoflavones are administered. In addition, it has similar activity to estrogens after menopause due to pregnancy and childbirth. In addition to inhibiting, osteoblasts increase the osteoblasts that make bones.

According to the NBC medical journalist Annot's "Breast Cancer Prevention Diet", soybeans, which consume 35 grams per day on the average of Asians, may be the most powerful preventive measure for breast cancer. Genistein, found only in soybeans, plays an important role in the development of breast cancer. It inhibits the carcinogenic effects of female hormone estrogen. In this regard, breast cancer rates in Chinese women who ate large amounts of soybeans decreased by 50%, while breast cancer was twice as high when soybeans were consumed less than once a week.

Linseed (Linum Usitatissium ( Linseed ) Seed) grows in high altitudes in cold winters and has a life span of up to 4 meters below ground to absorb soil nutrients. Apigenin, beta-carotene, beta-sitosterol, camphorsterol, chlorogenic acid, cycloarthenol, lecithin, luteolin, myristic acid, squalene, stigmasterol, bitexin, amino acids, calcium, essential fatty acids, iron, magnesium, manganese, Phosphorus, Potassium, Sulfur, Vanadium, Zinc, Vitamins B1, B2, B3, B5, E, etc.-There are numerous nutrients, the three most important of which are dietary fiber, omega-3 fatty acids and phytochemical lignans to be. In particular, linseed contains linoleic acid and linolenic acid called unsaturated fats. Although the peculiarity of the fat component in flaxseed is plants, it contains a lot of unsaturated fats (omega-3) in fish and animals. Unsaturated fatty acids, called flaxseed omega-3, help regulate the metabolism in our bodies, strengthen the walls of blood vessels, and release triglycerides out of the body to lower cholesterol levels and relieve inflammation and pain. It becomes a material for the brain and retina, and a raw material for synthesizing DHA, which plays a role in the brain's active energy. Unsaturated fatty acids have the effect of inhibiting platelet aggregation, preventing thrombus formation, thus reducing the death rate from heart disease. Omega-3 in flaxseed is a natural food, which is safe and efficient compared to animal omega-3 because it is vegetable and has good safety and absorption rate. Flaxseed contains a large amount of oke-4 fatty acids, and the effects of omega-3 fatty acids, which are also found in fish, have been shown to prevent breast cancer in women from Greenland's Eskimo, who eat the most omega-3 fatty acids on the planet. It turned out not to be determined. However, flaxseed oil has no estrogen effect and has been studied to have an estrogen effect when ingested.

Honey has long been said to be a long-lived predecessor, and is recognized as the best high-level, comprehensive nutritional food in modern medicine. Honey is also a great beauty ingredient for nourishing youth and beautiful skin. In particular, honey is known to have a very strong bactericidal power. In particular, the propolis of honey is described in Propolis in natural therapeutics, Maloine Editeur SA, Paris, Fance (1983); Fragrance Journal, No. 83, pp. 20-28 and pp. 36-43 (1987). As a bee is a dendritic substance stored in a honeycomb, which contains resins, beeswax, essential oils, pollen, and flavonoids, and has been used as a folk remedy since ancient times. The main action of propolis is antibacterial action, antioxidant action, anti-inflammatory action, local anesthesia action, virus growth inhibition action, immunomodulatory action, and the like as the main effective ingredients of the polycarbonate, aromatic carboxylic acid, aromatic aldehyde, etc. are known. . Propolis is collected from sap and flowers of trees by bees, and is used for self-defense of honey bees or repair of beehives.It is reported to have antibacterial, antifungal, antiviral, anti-tumor and wound healing effects. have. The ingredients of propolis vary according to the origin and the main collector, mainly resin (50 ~ 55%), beeswax (30%), essential oil (8 ~ 10%), pollen (5%), various organic components and minerals. It consists of components. Propolis has a strong antimicrobial effect against Gram-positive bacteria, which is reported to be exerted by flavonoids and aromatic acids present in resins. (Marcucci, MC, Ferrers, F., Garcia-Viguera, C., Bankova, VS, Castro, SLD, Dantas, AP, Valente, PHM, Paulino, N. Phenolic compounds from Brazilian propolis with pharmacological activities) , J. Ethnopharmacol, (2001) 74; 105-112). In traditional Egypt, honey was used to treat wounds and has been studied to inhibit bacterial growth (Journal of Applied Microbiology, 2002; 93: 857-863).

The present inventors have anti-inflammatory, immune-improving, and hormonal-modulating effects, and the effects of the phenugrig, horseback riding, pomegranate, soy isoflavone, flaxseed, and honey extracts, which have been finally selected, are significantly increased. It was confirmed (Fig. 2 and 4).

Thus, in one aspect, the present invention provides a cosmetic composition having a breast muscle alleviating effect comprising phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract.

As used herein, the term "extract" refers to an active ingredient isolated from natural products, the present invention is phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed and hormonal regulation, improving immunity, anti-inflammatory, antibacterial Provide honey extract. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or any form formulated using the same.

In the present invention, the term "Phenigrig ( Trigonella Foenum - Graecum ) "includes all organs of natural and hybrid lung nugricks, such as roots, stems, leaves, flowers, seeds, and preferably means seeds of fenyugri. the term "Equestrian (Cimicifuga racemosa)" means any authority, natural and hybrid Riding for example, contains all the roots, stems, leaves, flowers, seeds, and preferably refers to the roots of riding in. in the present invention, The term, "pomegranate ( Punica) Granatum) and "means to include both the peel and fruit of natural and hybrid pomegranates present invention the term" soy isoflavones (Soy Isoflavones) "is meant to include all of the isoflavones derived from natural and hybrid soybean. In the present invention, the term " Linum Usitatissium ( Linseed ) Seed )" means fat contained in the seeds of flax.

Cosmetic composition comprising phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed and honey extracts of the present invention has a synergistic anti-inflammatory, increased immunity compared to each alone extract. Extracts containing all phenegrigs, horseback riding, pomegranate, soy isoflavones, flaxseed and honey had a concentration-dependent NF-kB promoter inhibitory activity and showed significantly higher inhibitory activity than each single plant extract of the same concentration.

In addition, the composition comprising the extract mixture of the present invention has a synergistic female hormone balancing effect as compared to each single extract. It was confirmed that extracts containing all phenegrigs, horse riding, pomegranate, soy isoflavones, flaxseed and honey had more progesterone receptor activity than each single extract.

In addition, the composition of the present invention has antibacterial activity. The mixed composition of the present invention inhibited the growth of microorganisms such as Escherichia coli, Bacillus substilis with higher activity than honey extract known to have strong antibacterial activity.

In addition, the compositions of the present invention do not exhibit cytotoxicity. The composition comprising the extract mixture of the present invention did not affect the viability of the cells.

Lotion of the cosmetic composition of the present invention having more synergistic anti-inflammatory, increased immunity, hormonal regulation effect and antimicrobial activity when used alone when mixed with phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract as described above As a result of formulating and looking at the effect, it was confirmed that the improvement of the firming and firing up the chest. In addition, as a result of examining the degree of fever improvement of the lotion formulation by measuring the body heat of the breast, the heat of the breast was reduced in 65%.

Phenegrig, horseback, pomegranate, soy isoflavones, flaxseed and honey extracts of the present invention may be simultaneously extracted by mixing one or more ingredients. Preferably, the individual components are extracted and mixed under optimum conditions. The extracted liquid is preferably filtered and the filtrate is concentrated to be used.

Extract preparation process of the present invention comprises the steps of grinding natural products; Extracting with a solvent; Filtering the extract; And concentrating the filtrate.

Prepared natural products are extracted by grinding to 10 to 200 mesh size.

As the extraction solvent, organic solvents such as water and alcohols, or mixed solvents thereof are used, and the active ingredient of the herbal medicine is processed in a condition where the active ingredient of the herbal medicine is not destroyed or minimized and is extracted by heat extraction or cold extraction. The extract of the present invention is characterized by increasing the extraction efficiency of the active ingredient of natural products using the ultrasonic extract.

In the case of water extraction, it is preferable to add water to the milled natural product of the present invention and to heat it at 50 ° C to 100 ° C for 30 minutes to 42 hours. Properly adjust the temperature and time so that the active ingredient prevents destruction and that the active ingredient is extracted sufficiently.

When extracted with an organic solvent, methanol, ethanol, isoprotanol, butanol, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N-dimethylformamide), DMSO (dimethyl sulfoxide), It can be extracted with an organic solvent at room temperature or by using 1,3-butylene glycol aqueous solution, propylene glycol or the like. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient may be different, so select an appropriate organic solvent. Preferably extracted at 20 ℃ to 30 ℃, left for about 12 hours to 48 hours.

Phenegrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract of the cosmetic composition of the present invention is preferably included in an amount of 0.001 to 5% by weight based on the total weight of the cosmetic composition.

The blending ratio of phenegrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extracts is not particularly limited, but in the case of phenegrig, horse riding, pomegranate and flaxseed, 1 to 15 times the amount of each of these same dry weights. An extract extracted by adding the same weight of the extraction solvent, an extract extracted by adding the same weight of the extraction solvent and the extraction solvent with respect to 1/2 weight of honey propolis of the weight of each component, and the phenugrick, horse riding, pomegranate or flaxseed Soybean isoflavin extract extracted by adding about 0.05-1.0 times the weight of the extraction solvent to about 100 times the weight of soybean (soybean), the mixing ratio of the volume ratio, fenugreek, horse riding, pomegranate, soy isoflavones, The ratio of flaxseed and honey extracts is from 1 to 3: 3 to 5: 3 to 5: 0.5 to 1.5: 1 to 3: 0.5 to 2, more preferably from 2: 4: 4: 1: 1: 2: 1. Mixing with You can use

The breast muscle relief cosmetic composition of the present invention is not particularly limited in the formulation, for example, may have a formulation such as lotion, nutrition lotion, nutrition cream, massage cream, essence, pack. In addition, in the composition for breast palliative cosmetics of each formulation, other ingredients other than the above-mentioned phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed, honey mixed extract may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. .

The components to be incorporated into the cosmetic composition of the present invention include oils and fats, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, antioxidants, pH adjusters, alcohols, pigments, perfumes, and the like.

The fat or oil component may be an ester fat, a hydrocarbon fat, a silicone fat, a fluorine fat, an animal fat, a vegetable fat or the like.

The moisturizing agent may be a water-soluble low molecular moisturizer, a fat-soluble low molecular moisturizer, a water-soluble polymer moisturizer, a fat-soluble polymer moisturizer or the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl esters, hydroxystearate choleteryl esters, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As the surfactant, nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and the like can be used.

The alcohol may be a higher alcohol such as cetyl alcohol.

In a specific embodiment of the present invention provides a lotion (skin lotion) in Preparation Example 1, nutrition lotion in Preparation Example 2, nutrition cream in Preparation Example 3, essence in Preparation Example 4, the specific cosmetic composition and manufacturing process of Preparation Example 5.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the present invention is not limited by the following examples.

Experimental Example  One

NF-kB luciferase reporter activity test was used to investigate whether or not to suppress immune-related responses from 20 kinds of plant extracts reported to have anti-inflammatory and immune-improving effects. NF-kB promoters have been reported to play an important role in the production of immune-related cytokines. In order to measure NF-kB luciferase activity, the following method was used.

Way

Dispense 1 × ¹⁰ 6 cells into 6 wells of THP-1 cells, which are human monocytes, and then use NF-kB luciferase reporter plasmid DNA (NF-) using a superperfect transfection reagent. kB luciferase reporter plasmid DNA) was transfected. After 24 hours after transfection, lipopolysaccharide (LPS) (100 ng / ml) was treated to activate THP-1 cells and each extract was treated at 1% concentration. After 24 hours, cells were collected and luciferase activity was measured using a luminometer.

result

According to the results of Table 1, NF-kB luciferase inhibitory ability was determined by the Phenygrig seed extract ( Trigonella Foenum - Graecum). Seed Extract, Horse Riding Extract ( Cimicifuga Racemosa Root Extract, Punica Extract Granatum Extract), soy isoflavones (Soy Isoflavones), flax seed extract (Linum Only six extracts, such as Usitatissium ( Linseed ) Seed Extract), were clearly visible, while the other extracts except grapes showed no inhibitory effect or very weak. The results are summarized as follows (Table 1).

Extraction Type NF-kB promoter inhibition Phenegrig Extract ( Trigonella Foenum - graecum Seed Extract) +++ Horse Riding Extract ( Cimicifuga Racemosa Root Extract) +++ Pomegranate Extract ( Pucnica Granatum Extract) +++ Soy Isoflavones Isoflavones ) +++ Flaxseed Extract ( Linum Usitatissium ( Linseed ) Seed Extract) +++ Honey +++ Soybean Extract ( Polygonum cuspidatum Extract) ++ Grape Extract ( Vitis Vinifera Extract) ++ Acid extract ( Orixa japonica Extract) - Shea extract ( Evodia daniellii Extract) + Ganoderma lucidum extract ( Ganodema tsugae Extract) ++ Sewage Extract ( Evodia officinalis Extract) - Citron extract ( Citrus junos Extract) - Derma extract ( Citrus grandis Extract) - Ogapi extract ( Acanthopanax gracilistylus Extract) + Natural Herb Extract ( Zanthoxylum bungeanum Extract) + Bark Extract ( Zanthoxylum piperitum Extract) + Cypress Extract ( Thuja orientalis Extract) - Tangerine extract ( Poncirus trifoliate Extract) - Yellow Wall Extract ( Phellodendron amurense Extract) -

Experimental Example  2

Hormone control effect was measured using the Non-Reproductive tract target tissue response test for the plant extract of Experiment 1.

Phytoestrogens have a function similar to that of estrogens, and supplement the estrogens necessary for performing estrogen-like functions when the body lacks estrogens. On the other hand, phytoestrogens are similar to estrogen receptors when the estrogens are excessively secreted. ) To control the effects of female hormones in the body by preventing excessive estrogen reactions. MCF-7 cell proliferation in vitro (aka E-screen assay) is the most widely used non-reproductive tract target tissue response test. MCF-7 cell line is a hormone-dependent human metastatic breast cancer (Soule et al ., 1973), which has an estrogen receptor (ER) and inhibits cell proliferation by various cell growth responses to estrogens and by anti-estrogens tamoxifen and CI-628. The standard assay employs charcoal-dextran-stripped female human serum and a six-day incubation period and destroys MCF-7 cells to measure proliferation.

Way

After destruction of MCF-7 cells incubated with plant extracts, the amount of expressed progesterone receptor (Progesterone Receptor) mRNA is measured using RT-PCR. Preincubate MCF-7 cells (5X10 ⁶ ) in an strogen-free medium. After adding 1% of the plant mixture extract to the cells and incubating for 4 days, mRNA was isolated from the cells using RNAqueous Kit, quantitated at 260 nm, and RT PCT was performed to measure progesterone receptor expression levels.

result

In the MCF-7 cells, which are breast cancer cells, phenogrig, horseback, and pomegranate extracts, which are known to contain phytoestrogens, were observed in the stimulation of progesterone receptors by hormonal regulation by various extracts with increased estrogen activity. Hormonal regulatory effects on the back were observed. The results are summarized as follows (Table 2).

Extraction Type Expression of PR Phenegrig Extract ( Trigonella Foenum - graecum Seed Extract) +++ Horse Riding Extract ( Cimicifuga Racemosa Root Extract) +++ Pomegranate extract ( Punica Granatum Extract) +++ Soy Isoflavones Isoflavones ) +++ Flaxseed Extract ( Linum Usitatissium ( Linseed ) Seed Extract) ++ Honey + Soybean Extract ( Polygonum cuspidatum Extract) + Grape Extract ( Vitis Vinifera Extract) + Acid extract ( Orixa japonica Extract) - Shea extract ( Evodia daniellii Extract) + Ganoderma lucidum extract ( Ganodema tsugae Extract) + Sewage Extract ( Evodia officinalis Extract) - Citron extract ( Citrus junos Extract) - Derma extract ( Citrus grandis Extract) - Ogapi extract ( Acanthopanax gracilistylus Extract) + Natural Herb Extract ( Zanthoxylum bungeanum Extract) + Bark Extract ( Zanthoxylum piperitum Extract) + Cypress Extract ( Thuja orientalis Extract) - Tangerine extract ( Poncirus trifoliate Extract) - Yellow Wall Extract ( Phellodendron amurense Extract) -

In the present invention according to the experiment 1 and 2 in the present invention fenugreek extract ( Trigonella ) as a plant extract for improving breast congestion (breast meat) of women Foenum - graecum Seed Extract, Horse Riding Extract ( Cimicifuga Racemosa Root Extract), pomegranate extract (Punica Granatum The Extract), soy isoflavones (Soy Isoflavones), flax seed extract (Linum Usitatissium (Linseed) Seed exrtact ), Honey (Honey) extracts were Selection

Fenugreek extract of the present invention ( Trigonella Foenum - graecum Seed Extract, Horse Riding Extract ( Cimicifuga Racemosa Root Extract), pomegranate extract (Punica Granatum Extract), soy isoflavones (Soy Isoflavones), flax seed extract (Linum Usitatissium (Linseed) Seed exrtact ), Honey (Honey) mixing the extract and penyu Greek, horseback riding, pomegranate, soy isoflavones cosmetics containing flaxseed, honey mixed extract The manufacturing method is as follows. In the cosmetic composition of the present invention, phenugrick extract, horse riding extract, pomegranate extract, soy isoflavone extract, flaxseed extract, honey extract mixture can be added to the cosmetic in an amount of 0.1% to 10% by weight of the liquid, and also a cooling condenser The solvent can be added to the cosmetic in an amount of 0.001 to 5% by weight, preferably 0.01 to 3% by weight, as the dry weight thereof, while recovering the solvent evaporated using the distillation apparatus.

Example  One : Phenegrig  Extract Preparation Method

After grinding 100g of dried phenugrig into 10 ~ 200 mesh size with a herbal medicine grinder, 500ml of 80% (v / v) ethanol aqueous solution was added to ultrasonic vibration and ultrasonic vibration was performed to extract the active ingredient including the cell membrane in ethanol. After cooling at room temperature for 24 hours, sterilization was filtered using 0.40 μm filter paper. The concentrated extract obtained by concentrating the filtered extract under reduced pressure at 45 ° C. was used as an extract.

Example  2: manufacturing method of equestrian extract

After 100 g of dried horseback riding was pulverized to a size of 10 to 200 mesh using a herbal medicine grinder, 500 ml of 80% (v / v) ethanol aqueous solution was added to ultrasonic vibration, and ultrasonic vibration was performed to allow the active ingredient including the cell membrane to be extracted in ethanol. After cooling for 24 hours at room temperature, sterilization was filtered using 0.40㎛ filter paper. The concentrated extract obtained by concentrating the filtered extract under reduced pressure at 45 ° C. was used as an extract.

Example  3: pomegranate extract manufacturing method

The method for preparing the pomegranate extract used in the present invention is as follows.

After grinding 100 g of dried pomegranate into 10 to 200 mesh size using a herbal medicine grinder, 500 ml of 80% (v / v) ethanol aqueous solution was added to ultrasonic vibration, and ultrasonic vibration was applied to extract the active ingredient including the cell membrane in ethanol. After cooling for 24 hours at room temperature, sterilization was filtered using 0.40㎛ filter paper. The concentrated extract obtained by concentrating the filtered extract under reduced pressure at 45 ° C. was used as an extract.

Example  4: soy isoflavone manufacturing method

A constant 3 l was added to 10 kg of soybeans, and then soaked for about 4 hours at a temperature of 20 to 30 ° C. This was pulverized with a mixer and filtered through a 200 mesh filter network to obtain 25 L of soy milk emulsion of about 10%. It was heated at 93-95 ° C. for 30 minutes, then cooled to 85 ° C. to coagulate and precipitate the protein by adding gluconodelta-lactone, magnesium chloride and calcium sulfate to separate the supernatant. 5% of ferrite was added to the supernatant, and the filtrate was passed through a reverse osmosis membrane (RO Membrane: CA865PP from De Danske Sukkerfabrikker Co., Ltd.) at 5 to 30 ° C., and inorganic salts such as magnesium chloride used for protein coagulation precipitation. And monosaccharides were removed to give 200 ml of purified concentrate. The purified concentrate was adsorbed resin and flowed to a column of XAD7 (Germany) 300 to 500 liters at a flow rate of 1 to 4 liters per minute so that isoflavones were adsorbed onto the resin. After distilled water was sufficiently flowed into the column through which all the extracts passed, the resin was washed, and then isoflavone was recovered by flowing 70 to 95% ethanol. The end of recovery was based on the case where the absorbance falls below a certain level using isoflavone having a absorbance of 260 nm as a reference material. The recovered ethanol eluate was concentrated under reduced pressure at a temperature of 40 to 80 ° C. to obtain a concentrate. The concentrate was dried under reduced pressure at 30 to 80 ° C. to obtain a pale yellow yellow isoflavone.

Example  5: flaxseed extract manufacturing method

After grinding 100 g of dried flaxseed to 10 to 200 mesh size using a herbal pulverizer, 500 ml of 80% (v / v) ethanol aqueous solution was added to ultrasonic vibration, and ultrasonic vibration was applied to extract the active ingredient including the cell membrane into ethanol. After heating for 20 hours at 50 ~ 90 ℃, it was cooled to room temperature and then filtered using a 0.40㎛ filter paper. The filtered extract was concentrated under reduced pressure at 45 ℃ was used as an extract.

Example  6: method of manufacturing honey extract

500 g of 95v / v% ethanol was added to 50 g of a mixture of honey and propolis (1: 1), and the mixture was shaken at room temperature for 24 hours, followed by centrifugation. The ethanol layer was separated from the centrifuged liquid and concentrated under reduced pressure in vacuo to prepare a propolis ethanol extract. Distilled water was added to the remaining liquid from which the ethanol layer was removed, and the mixture was vibrated and stirred at room temperature for 4 hours, and then the aqueous fraction was separated and concentrated under reduced pressure in vacuo to prepare an extract from which wax was removed.

When the extraction according to the embodiment was compared with the extract extracted by changing the solvent, the components were the same, but there was a slight difference in the amount extracted by different solvent, but it can be confirmed that it is not particularly problematic. there was. However, in consideration of efficiency, it was more convenient to use ethanol aqueous solution as a solvent.

Example  7. Phenegrig Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, Honey Blend Extract

For each of the extracts of Examples 1 to 6 prepared above, the results of Table 3 and Table 4 were obtained by performing the cell immunoactivity test of Experimental Example 1 and the minimum inhibitory concentration test of Experimental Example 5. According to the experiments of Table 3 and Table 4, the extraction mixture was prepared by selecting the optimum active concentration at the same concentration. Cells were activated by treatment with LPS (Lipopolysaccharide) and treated with extracts at various concentrations (0.5%, 1.0%, 2.0%, 4.0%, 8.0%), and the inhibitory effect of the extracts on the NF-kB promoter was confirmed (Table 3). . LPS * indicates that LPS was treated alone.

density NF-kB promoter inhibition Phenegrig riding Pomegranate Soy isoflavones flaxseed honey LPS * 26,597 26,597 26,597 26,597 26,597 26,597 0.5% 26,594 26,554 26,577 24,215 26,587 26,596 1.0% 20,417 26,417 26,511 18,419 21,486 26,514 2.0% 12,214 25,847 25,477 17,975 20,324 25,479 4.0% 12,248 22,415 22,154 17,955 14,817 25,313 8.0% 12,211 22,419 21,279 16,854 13,979 24,367

(LPS: lipopolysaccharide)

Escherichia coli 0.25% 0.5% One% 2% Phenegrig Extract + + + + Equestrian extract + + + + Pomegranate extract + + + + Soy isoflavones + + - - flaxseed + + + - Honey extract + + - -

According to the experimental results, the extract mixture was prepared to contain phenugrick, horse riding, pomegranate, soy isoflavone, flaxseed, and honey extract concentrate in a ratio of 2: 4: 4: 1: 2: 1.

Production Example  One.

Prescription example of a lotion (skin lotion) in the cosmetic composition containing the phenugrig extract, horse riding extract, pomegranate extract, soy isoflavone extract, flaxseed extract, honey extract mixture of Example 7 is as follows.

number Raw material Content (% by weight) One Phenegrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, Honey Extraction Mixture (Example 7) 1.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Remaining amount

<Production method>

11, 2, 3, 4, and 8 are sequentially added and stirred to dissolve. Then, 5 is heated to 60 ° C. to dissolve, and 10 is added to dissolve and 11 is added thereto. Finally, 1, 6, 7, and 9 are added and sufficiently stirred before aging.

Production Example  2.

Prescription example of nutritional lotion in the cosmetic composition containing the phenegrig extract, horse riding extract, pomegranate extract, soy isoflavone extract, flaxseed extract, honey extract mixture of Example 7 is as follows.

number Raw material Content (% by weight) One Phenegrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, Honey Extraction Mixture (Example 7) 1.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solbitan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Remaining amount

<Production method>

10, 11, 13, and 16 were mixed and stirred to be heated to 80 ~ 85 ℃, put into the manufacturing unit, and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 12 was 80 ~ 85 ℃ After dissolving by heating and emulsifying. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then 15 times were added, cooled to 45 ° C., and 14 times were added.

Production Example  3

Prescription example of nutrition cream in the cosmetic composition containing the phenugrig extract, horse riding extract, pomegranate extract, soy isoflavone extract, flaxseed extract, honey extract mixture of Example 7 is as follows.

number Raw material Content (% by weight) One Phenegrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, Honey Extraction Mixture (Example 7) 1.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglycerides 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Remaining amount

<Production method>

While stirring 12, 13, 14, and 16, and heating it between 80 ~ 85 ℃ put into the manufacturing unit and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 80 ~ After heating to 85 ° C. to dissolve, 15 was added and stirred, and added to the production part, followed by emulsification. After emulsification, the mixture is cooled to 35 ° C while stirring using a stirrer and 1 time is added to it, cooled to 25 ° C, and aged.

Production Example  4

Prescription example of the essence of the cosmetic composition containing the phenegrig extract, horse riding extract, pomegranate extract, soy isoflavone extract, flaxseed extract, honey extract mixture of Example 7.

number Raw material Content (% by weight) One Phenegrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, Honey Extract Mixture (Example 7) 1.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount

<Production method>

2, 3, 4, 5 and 6 are called nonionic amphiphilic lipids by homogenizing at a constant temperature. The nonionic amphiphilic lipids are mixed with 1, 7, 8, and 14, and homogenized at a constant temperature to pass through the microfluidizer, followed by the addition of 9 at a constant temperature, homogenized, and then again passed through the microfluidizer. Add 10, 11, 12, 13, and disperse to stabilize and mature.

Experimental Example  3

The cytotoxic effect using the cell culture of the extract mixture prepared in Example 7 was measured.

Way

The medium used for the cytotoxicity test (Mosmann, T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J. Immunol. Meth. 1983, 65, 55-63.) Contains 10% FBS. Seed human fibroblast cell line (ATCC, CRL-2076) in DMEM at 24x plate at 1X10 ⁵ cells / ml. After 24 hours of seeding, the medium is replaced and the pneumatic, horseback, pomegranate, soy isoflavones, flaxseed and honey extract mixtures are added at different concentrations and incubated in a 37 ° C, 5% CO ₂ incubator for 3 days. After adding 1 ml / well of MTT solution at 37 ° C. for 4 hours in a CO ₂ incubator, the medium is removed, and 200 μl of DMSO is added thereto, and the absorbance is measured at 550 nm.

result

Concentrations of Phenegrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed and Honey Extract Mixture (%) Cell survival rate (%) 0.25 101 0.5 100 One 101 5 92 10 93

At various concentrations, there was no inhibition in cell growth for phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extract mixtures. This indicates that the mixed extract of the present invention can be applied as a safe cosmetic composition.

Experimental Example  4

For the mixed extract prepared in Example 7, the effect of cellular immune activity using the NF-kB activation inhibition test was measured.

Way

The experiment was carried out in the same manner as in Experiment 1.

result

Immune-related activities of the Phenygrig, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extract mixtures were observed using NF-kB luciferase experiments. In addition, comparative experiments were performed for the treatment of phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract mixtures at the same concentration and 1% of each plant extract alone.

Phenygrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract mixtures can be seen to inhibit NF-kB luciferase activity in a concentration dependent manner. In addition, when compared to the treatment of 1% of each single plant extract with phenugrick, horseback riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture, Phenugrick, horseback riding, pomegranate, soybean isoflavone, flaxseed, honey The extract mixture showed high inhibitory activity at the same concentration (FIG. 2). These results were verified by synergy between the extracts that make up Phenygrig, Horse Riding, Pomegranate, Soy Isoflavones, Flaxseed, and Honey extract mixture.

Experimental Example  5

The antimicrobial effect of the extract mixture prepared in Example 7 was measured using a minimum inhibitory concentration (MIC) test.

MIC test This is a method of measuring the growth of test bacteria after adding antimicrobial effect to the concentration step in liquid medium, inoculating them with various bacterial strains, shaking culture for 24 hours at 35 ℃.

Way

After incubating each microorganism to be tested in 10 ml of brain heart infusion broth for 24 hours at 37 ° C., the microorganisms were inoculated to make 10 ⁴ cells in 3 ml of the culture medium. Phenegrig, horseback riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture and a single honey extract are added to each medium at different concentrations. Incubate the prepared test tube at 37 ° C. for 24 hours and observe the growth of microorganisms.

result

Fungus 20 ug / ml 50 ug / ml 100 ug / ml 200 ug / ml 400 ug / ml 1 mg / ml honey Esherichia Coli + + + + + - Bacillus substilis + + + + + - Mixed extract Esherichia Coli + + + + - - Bacillus substilis + + + - - -

As shown in Table 6, the fenugreek, horse riding, pomegranate, soy isoflavones, flaxseed, honey mixture extract according to the present invention was found to be 200ug / ml Bacillus, 400ug / ml E. Coli. This is a result proved that the mixed extract of the present invention has a microbial growth inhibitory effect. It was also observed that the antimicrobial activity showed higher antimicrobial activity in the extract mixture compared to the use of honey extract alone, which is known as a single antimicrobial extract.

Experimental Example  6

In order to verify the results in Experimental Example 5, the antimicrobial effect on the microorganisms of the extract mixture was observed by the disc diffusion method. The Disk Diffusion Method inoculates evenly the strain of the test strain with a cotton swab on the surface of the basal agar medium and places an antimicrobial disk on it. During the incubation time, the antimicrobial agent in the disc diffuses into the surrounding agar, so the antibacterial agent is distributed in high concentration in the agar immediately around the disc, but the antimicrobial agent is distributed in low concentration in the agar far from the disc. The antibacterial concentration gradient of the agar thus forms a suppression zone for bacterial growth. That is, the concentration of the antimicrobial agent in the agar at a certain distance from the disk becomes the MIC for the test strain, and the strain does not grow in the agar inside this point, but the strain grows outside this point.

Way

4-5 colonies that are well separated from the pure culture plate medium are selected and inoculated into the enrichment medium with platinum. Incubate the cultures at 37 ° C incubator with sterilized physiological saline or liquid medium until the bacteria grow above the concentration of standard turbidity, equal to the standard turbidity (in vitro). Within 15 minutes after adjusting the concentration of the bacterial culture solution, the sterilized cotton swab is soaked in the bacterial culture solution and twisted while rotating on the inner wall of the test tube to remove excess bacterial solution. Rub the cotton swab moistened with the bacteria on the dry agar plate. Close the plate lid and leave it for 3-5 minutes to let the surface absorb moisture, then use a disk dispenser to place the required disk on an evenly inoculated agar plate medium, and gently press the center of the disk to attach it completely to the agar surface. Each disc contains a cream control that does not contain phenegrick, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture, and a phenegrick, horse riding, pomegranate, soy isoflavone, flaxseed, honey extract mixture The plate on which the disk containing the cream (A), the lotion (B), and the skin (C) is collected is left to stand for about 30 minutes, and then incubated by placing the plate lid downward in a 37 ° C incubator.

result

In Figure 3 it was verified that the antimicrobial effect for various preparation examples of skin, lotion, cream containing fenugreek, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture as shown in Figure 3 (Fig. 3).

Experimental Example  6

For the mixed extract prepared in Example 7, the effect of hormonal regulation using the Non-Reproductive tract target tissue response test was verified.

Way

It carried out similarly to Experimental Example 2.

result

In the MCF-7 cells, which are breast cancer cells, the progesterone receptor activity was promoted by the hormonal regulation of various extracts by increasing the estrogen activity. Could. However, it was observed that the same concentration of mixed extract Phenrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey mixed extract has a higher hormonal regulatory activity for a single extract (Fig. 4).

Experimental Example  7

The mammary gland developmental effect of hormonal control of lotion formulations containing phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extract mixtures was measured as an improvement in firing up of the subject's chest.

Way

A lot of formulations containing and without phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extract mixtures were used to confirm breast improvement. In a clinical trial, 20 women aged 20-40 who had no previous breast history were divided into two groups of two lotion formulations prepared on the chest for 12 weeks by applying each formulation to the chest once a day. .

result

FIG. 5 is a photograph of breast change applied to a woman's breast for 12 weeks with a lotion formulation containing phenegrig, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extract mixture. Phenigrig, horse riding, pomegranate, soy isoflavones, flaxseed, and honey extracts were applied to the chest for 12 weeks, which showed elasticity and improved firing up. The degree of improvement was measured by the angle of the nipple, based on the degree of elevation of the chest of the woman's chest.

Before use 110 ° after use 119 ° Improvement 8.2%

Experimental Example  8

Hormonal secretion imbalances caused by breast thigh, breast congestion, and menopausal disorders are common symptoms such as pain, fever, congestion, infection, and inflammation in the breast. For women with these symptoms, we measured the effect of improving fever due to the improvement of inflammation by using a lotion formulation applied with phenugrig, horse riding, pomegranate, soy isoflavone, flaxseed, and honey extract mixture.

Way

A lotion containing 3% of a phenugrick, horse riding, pomegranate, soy isoflavone, flaxseed, and honey extract mixture was prepared and used in the test. Clinical trials consisted of applying a 20--40 year old woman without a history of breast massage twice a day to a lotion. In addition to the response from the user's questionnaire before and after the use of the product, the change in the temperature of the breast before and after use was measured by means of a thermometer on the chest.

result

so so is average good very good  Number of subjects  2  4  11  3

A survey was conducted on 20 subjects who applied the lotion formulation prepared on the chest for 1 week by massaging twice a day. For good results, 15% felt very good improvement.

Breast heat was measured by a thermometer to measure the improvement of breast fever after 20 weeks of use in a patient who applied the lotion formulation to the breast for 1 week by massaging twice a day. . 65% of the body heat of the induction resulted in a decrease in the body heat of the breast (FIG. 5).

decrease No change The number of the subjects 13 people 7 people Improvement 65% 35%

Phenegrig, horse riding, pomegranate, soy isoflavones, flaxseed, honey extract mixture and cosmetics containing the same according to the present invention has the effect of improving the body fat by controlling female hormones, improving immunity, anti-inflammatory, antibacterial, It is a safe cosmetic that does not have cytotoxicity and has an excellent effect on breast fat. It was possible to use it in various cosmetics.

Claims (7)

A cosmetic composition having a breast tenderness-reducing effect comprising phenugrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract. The cosmetic composition according to claim 1, wherein the total amount of phenegrig, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract is included in an amount of 0.001 to 5% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is used in the formulation of softening cream, nutrient cream, nourishing cream, massage cream, essence or pack. According to claim 1, wherein the extract extracted by adding the same weight of the extraction solvent of 1 to 15 times to the same dry weight of each of the pneugric, horse riding, pomegranate and flaxseed, and the honey of 1/2 weight of the weight of each component Extract the soy isoflavone extract extracted by adding the same extraction solvent as the extraction solvent, and adding 0.05 to 1.0 times the weight of the solvent to 100 times the weight of the phenegrig, horse riding, pomegranate or flaxseed. And the mixing ratios of the volume ratios are 1 to 3: 3 to 5: 3 to 5: 0.5 to 1.5: 1 to 3: 0.5 to 2, respectively, for fenyugrik, horse riding, pomegranate, soy isoflavones, flaxseed and honey extracts. Cosmetic composition containing in proportion. The cosmetic composition according to claim 4, wherein the mixing ratio of the phenugrick, horse riding, pomegranate, soy isoflavones, flaxseed and honey extract is 2: 4: 4: 1: 1: 1. The method of claim 4 or 5, wherein the extraction solvent is water, lower alcohol having 1 to 4 carbon atoms, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N- dimethylformamide), DMSO ( Dimethyl sulfoxide), 1,3-butylene glycol aqueous solution, propylene glycol, and a mixed solvent thereof, and the phenegrig, horse riding, pomegranate, flaxseed and honey extracts are 50-100 ° C., respectively. Cosmetic composition that is extracted by heating for 3 to 24 hours. The method of claim 4 or 5, wherein the extraction solvent is water, lower alcohol having 1 to 4 carbon atoms, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N- dimethyl formamide), DMSO ( Dimethylsulfoxide), 1,3-butylene glycol aqueous solution, propylene glycol, and a mixed solvent thereof, and the phenegrig, horse riding, pomegranate, flaxseed and honey extracts are each 4 ~ 30 ℃ Cosmetic composition that is extracted by dipping for 3 to 20 days.

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