KR101952695B1 - Composition having anti-wrinkle effects using the natural plant extract and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a wrinkle-improving cosmetic composition using natural materials, and cosmetics comprising the same. According to the present invention, the wrinkle-improving cosmetic composition has: skin protecting and wrinkle improving effects by mixing a black garlic extract, a lotus root extract, a seaweed extract, a motherwort extract, a Chinese matrimony vine extract, a Lycii Radicis Cortex extract, and a yacon leaf extract at a predetermined mixing ratio to prepare a mixture, thereby obtaining medicinal effects of natural extracts; further enhances a wrinkle improving function by additionally adding a first mixture prepared by mixing a flaxseed extract, a grape seed extract, an Orostachys japonicus extract, a pomegranate seed extract and an Oenanthe javanica extract, a second mixture prepared by mixing a Sanguisorba officinalis extract, a spirulina platensis extract, a fermented red ginseng extract, a Morus alba leaf extract and a safflower extract, a third mixture prepared by mixing a Rubus coreanus extract, a gastrodia rhizome extract, a Codonopsis lanceolata extract, a sophora flavescens extract and a Paeonia lactiflora extract, and a fourth mixture prepared by mixing a rice bran extract, a Caesalpinia sappan Lignum extract, a dictamnus root extract, a Phellinus linteus extract and a Momordica charantia extract to the mixture; and can extend storage and usage lifetime of wrinkle-improving cosmetics by adding a fifth mixture prepared by mixing a Zanthoxylum schinifolium extract, a moss extract, a Pulsatilla koreana Nakai extract and a grapefruit seed extract to the mixture.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for improving wrinkles using a natural substance, and a cosmetic composition containing the same. BACKGROUND ART < RTI ID = 0.0 >

The present invention provides skin protection and wrinkle-reducing effects by the pharmacological effect of natural extracts by mixing black gum extract, rootstock extract, seaweed extract, motherwort extract, gingival extract, gingival extract and yacon leaf extract at a predetermined blending ratio,

Also, a first mixture prepared by mixing flaxseed extract, grape seed extract, wanshong extract, pomegranate seed extract, and fenugreek extract; A second mixture prepared by mixing an oat extract, a spirulina extract, a fermented red ginseng extract, a leaf extract, and a safflower extract; A third mixture prepared by mixing a bokbunja extract, a chewy gum extract, a dodok extract, a gosam extract, and a peony extract; A fourth mixture prepared by mixing a rice bran extract, a root extract, a white roots extract, a mushroom extract, and a Yeoju extract, is further added to further enhance the wrinkle-

A cosmetic composition containing a natural substance capable of prolonging the shelf life and service life of a wrinkle-reducing cosmetic by adding a fifth mixture prepared by mixing a mixture of a mixture of a water extract of Sanchos extract, a moss extract, a mung bean extract and a grapefruit seed extract, and a cosmetic product containing the same .

The most common symptoms of aging are facial sagging, reduced skin elasticity, and increased number of wrinkles. In order to prevent and improve such aging of the skin, improvement of dietary life, exercise, use of cosmetics to improve wrinkles, and the like are performed.

Consumers are increasingly expecting cosmetics to prevent or improve skin aging due to their desire to look younger and more beautiful even when their age increases.

Some wrinkle-improving cosmetics sold in the market in the past have excellent wrinkle-improving effect, but the stability of the material is deteriorated, and there are serious side effects such as skin discoloration and skin cancer incidence in use.

Therefore, there is a growing interest in natural cosmetics which can secure safety and stability in use and enhance the effect of improving wrinkles.

Accordingly, many natural cosmetics using natural extracts have been developed and introduced, but cosmetics containing natural extracts have disadvantages in that they are used and stored for a relatively short period of time compared to general cosmetics due to the nature of natural products. Therefore, the value of the product may be lowered even though it is an expensive product.

Korean Registered Patent No. 10-1239393 (Registration date 2013.02.26) Korean Registered Patent No. 10-1280483 (Registration date 2013.06.25) Korean Registered Patent No. 10-1509424 (Registration date 2014.04.01) Korean Registered Patent No. 10-1562560 (Registration date 2015.10.16) Korean Registered Patent No. 10-1614659 (Registration date 2014.04.15)

The present invention provides skin protection and wrinkle-reducing effects by the pharmacological effect of natural extracts by mixing black gum extract, rootstock extract, seaweed extract, motherwort extract, gingival extract, gingival extract and yacon leaf extract at a predetermined blending ratio,

Also, a first mixture prepared by mixing flaxseed extract, grape seed extract, wanshong extract, pomegranate seed extract, and fenugreek extract; A second mixture prepared by mixing an oat extract, a spirulina extract, a fermented red ginseng extract, a leaf extract, and a safflower extract; A third mixture prepared by mixing a bokbunja extract, a chewy gum extract, a dodok extract, a gosam extract, and a peony extract; A fourth mixture prepared by mixing a rice bran extract, a root extract, a white roots extract, a mushroom extract, and a Yeoju extract, is further added to further enhance the wrinkle-

A cosmetic composition containing a wrinkle improving cosmetic composition using natural substances capable of prolonging the shelf life and service life of a cosmetic wrinkle-improving agent by adding a fifth mixture prepared by mixing a mixture of an acid extract, a moss extract, a Baekjong extract, and a grapefruit seed extract; It is the object of the invention to provide.

In order to achieve the above object,

The present invention relates to a composition comprising 0.01 to 70 wt% of black garlic extract;

Lenticular root extract 0.01 ~ 70 wt%;

Seaweed extract 0.01 ~ 70 wt%;

Motherwort extract 0.01 ~ 70 wt%;

Gugija extract 0.01 ~ 70 wt%;

0.01 - 70 wt.

And 0.01 to 70 wt% of yacht leaf extract; and a cosmetic composition for improving wrinkles using the natural material.

Also provided is a wrinkle-improving cosmetic comprising a natural substance prepared by adding the cosmetic composition to the cosmetic composition in an amount of 0.1 to 50 wt% based on the total weight of the cosmetic composition.

The cosmetic composition for improving wrinkles using natural materials according to the present invention and cosmetics containing the same have the following effects.

First, the cosmetic composition according to the present invention can provide a high wrinkle-reducing effect while enhancing the stability of use such as skin stability by using a natural extract using a natural extract having excellent wrinkle-reducing effect.

Second, a first mixture prepared by mixing flaxseed extract, grape seed extract, wanshong extract, pomegranate seed extract, and fenugreek extract; A second mixture prepared by mixing an oat extract, a spirulina extract, a fermented red ginseng extract, a leaf extract, and a safflower extract; A third mixture prepared by mixing a bokbunja extract, a chewy gum extract, a dodok extract, a gosam extract, and a peony extract; A fourth mixture prepared by mixing rice bran extract, bonito extract, white pine bark extract, phellodendron mugwort extract, and mung bean extract is additionally added to provide a higher wrinkle-reducing effect.

Third, by using the fifth mixture composed of the mixture of Sancho extract, Moss extract, Baekduong extract and Grapefruit seed extract, it is possible to use the natural preservative which is free of skin irritation and ensure the safety of use, The problem of short storage period can be solved.

fourth. It has an advantage that the health state of the skin can be continuously maintained by the pharmacological action of the natural extract.

The above-described technical construction will be described in more detail.

As described above,

The cosmetic composition for improving wrinkles using a natural substance according to the present invention comprises:

Black garlic extract 0.01 ~ 70 wt%;

Lenticular root extract 0.01 ~ 70 wt%;

Seaweed extract 0.01 ~ 70 wt%;

Motherwort extract 0.01 ~ 70 wt%;

Gugija extract 0.01 ~ 70 wt%;

0.01 - 70 wt.

0.01 to 70 wt% of yacht leaf extract;

Hereinafter, natural extracts constituting the cosmetic composition for improving wrinkles will be described in more detail.

<Black Garlic Extract>

When garlic is aged in a high-temperature thermostat for a certain period of time, the garlic is changed into black all the way to the inside by the self-component of the garlic and the enzyme etc. This is called "black garlic".

As the raw garlic turns into black garlic, the unstable and odorous components of raw garlic are changed into a more stable and odorless water-soluble substance and organic sulfur compounds such as S-allylcysteine (SAC), S-allylmercaptocystein (SAMC) and tetrahydro- soluble substances such as diallyl sulfide (DAS), triallyl sulfide, diallyl disulfide (DADS) and diallyl polysulfides. Fructosyl-arginine inhibits the production of thiobarbituric acid reactive substances (TBARS) in the liver and cell damage caused by oxidized LDL. .

The black garlic extract is prepared by mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture at 100 ° C and 100 to 140 rpm for 1 to 5 hours, filtering the resulting extract, And concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours.

More specifically, 1000 g of black garlic was added to 10 L of distilled water, and the mixture was shaken at 100 rpm and 120 rpm for 2 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Root extract>

It is a perennial herb that grows in the ponds and swamps of India, China, Korea, Japan, and Siberia. Roots, lotus leaves, lotus, and livestock are widely used for edible and medicinal purposes.

The rootstock contains 80 ~ 85% moisture, 12 ~ 15% carbohydrate, and contains trace amounts of protein and fat. It contains amino acids such as arginine, tyrosine, and trigonelline as well as about 2% of asparagine. It contains a lot of phospholipid, lecithin, so that water and oil are mixed well. Lt; / RTI &gt;

The extracts were prepared by mixing the dried rootstock powder and ethanol in a weight ratio of 1: 5 to 30, and then dipping the extract at room temperature for 20 to 30 hours. The obtained extract was filtered, And concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours.

More specifically, dried root roots washed with water are dried and then ground to prepare a powder. 1,000 g of rootstock powder is immersed in 1 L of 50% ethanol and extracted at 23 캜 for 20 hours. The extracted extract was filtered with a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA with a vacuum rotation evaporator to obtain 1 L of an extract.

<Algae extract>

Seaweeds are known to produce toxic or growth-limiting substances for use in competition with other organisms and to produce substances of self-defense against living creatures that attach to surfaces, making them valuable for the development of natural antibiotics. From past to present, many studies have revealed the results of physiological activities such as antioxidation, antibacterial, anticancer, anti-inflammation and anti-blood coagulation of seaweed.

In the present invention, seaweeds such as seaweed, kelp, moxa, and rhubarb are mixed at the same weight ratio.

The algae extracts were prepared by drying the seaweed mixed with seaweed, kelp, moxa, and rhubarb in the same weight ratio at 58 to 62 ° C for 10 to 14 hours in a hot air drier, then pulverizing the dried powder and distilled water at a ratio of 1:15 to 20 And the mixture is heated and extracted at 100 ° C for 1 to 3 hours to obtain an extract. The extract is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

Specific examples of the algae extract include,

Dried seaweed, kelp, kelp, and rhubarb were dried at 60 ° C for 12 hours in a hot air dryer, and then 1,000 g of dried powder mixed with dried seaweed, kelp, moxa, and rhubarb were mixed with 15 L of distilled water Then, the extract which has been heated and extracted at 100 ° C for 2 hours is filtered with a filter paper.

The filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Momocho extract>

The motherwort (Leonurus japonicus H.) is a two-year-old herb belonging to Lamiaceae. As the main components, leucine, leonuridine, stachynose, stachydrine, flavonoid, rutin, protocatechol, benzoic acid, vitamin A and fat were found to be alkaloids.

In addition to activating the blood, the motherwort juice also removes toxic blood in the body. In addition, when the blood pressure is high and the blood pressure is high, It is said to be used to treat uterine hemorrhage, hematuria, hemorrhoids, hemorrhoids, and urinary incontinence when it is dizziness and high fever due to hemorrhage and severe pain.

The motherwort extract is prepared by mixing motherwort and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture at 100 to 140 rpm for 1 to 5 hours, centrifuging the extract at 2,000 to 4,000 rpm, After centrifugation for 30 minutes, the supernatant is filtered and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

More specifically, 1,000 g of dried mother liquorice powder was added to 15 L of distilled water, shaken at 100 DEG C for 2 hours, and then centrifuged at 3,000 rpm for 15 minutes to obtain a supernatant from which the debris was removed. The supernatant was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours under vacuum of 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of an extract.

<Gugija extract>

It is called Lycii fructus, the bark of the root is the gill pulp, the leaf is the Lycii foloum, and the young louse and the leaf are the ceiling gill.

Gugija refers to the dried fruit of mulberry trees collected from summer to autumn, and Gugija and Gigolpypa have been used for medicinal purposes. It contains a large amount of carotenoids, cholin, melliscic acid, zeaxanthin, physalien (dipalmityl-zeaxanthin), betain, beta-sitosterol, vitamin B1, rutin, linoleic acid and unsaturated fatty acids.

The gugija extract was prepared by mixing dry gugija and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture at 100 ° C and 100 to 140 rpm for 1 to 5 hours, centrifuging the extract at 3,000 to 5,000 rpm, After centrifugation for 30 minutes, the supernatant is filtered and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPA.

More specifically, 1,000 g of dry gum was added to 20 L of distilled water, shaken at 100 DEG C for 2 hours, and then centrifuged at 4,000 rpm for 20 minutes to obtain a supernatant from which the residue was removed. The supernatant was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours under vacuum of 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of an extract.

<Extract of Gigae Bark>

Lycii Cortex Radicis is the root bark of Gugija, and its gingiva has a unique flavor. Betaine, β-sitosterol glucoside, linoleic acid, scopoletin and kukoamine have been reported.

The extracts were mixed with 1: 5 ~ 30 parts by weight of ethanol and the mixture was shaken at 65 ~ 75 ° C at 100 ~ 120 rpm for 2 ~ 6 hours. The resulting extract was filtered, Under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours.

More specifically, 1,000 g of the dried crustacea powder is added to 10 L of 70% ethanol, and the mixture is extracted at 70 DEG C and 110 rpm for 5 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Yacon leaf extract>

The yacon leaves are native to Peru and Bolivia in the Andean region of South America. It is a perennial plant root (tuberous root) crop belonging to Asteraceae. It has been reported that phenolic compounds such as chlorogenic acid, caffeic acid and ferulic acid have antioxidant activity, antimicrobial activity, hypoglycemia and anti - diabetic activity and anticancer activity.

The major components of yacon leaves are 86% moisture, 0.7% protein, 13.1% carbohydrate, 0.3% ash, 1.1% dietary fiber and inorganic components of calcium, potassium and phosphorus. Yacon leaves contain large amounts of fructose, fructo-oligosaccharide (FOS), glucose, sucrose, and inulin, and fructo-oligosaccharides (FOS) in saccharides account for nearly 80%.

The yacon leaf extract was prepared by mixing the dried yacon leaves powder with ethanol at a weight ratio of 1: 5 to 30, shaking the mixture at 65 to 75 ° C at 100 to 120 rpm for 2 to 6 hours, filtering the resulting extract, And concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of -0.3 mPA.

More specifically, 1,000 g of dried yacon leaf powder is added to 10 L of 70% ethanol and extracted at 70 DEG C and 110 rpm for 5 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The cosmetic composition for wrinkle improvement according to Example 1 is prepared by mixing black garlic extract, root lily extract, seaweed extract, motherwort extract, gugija extract, ginger bark extract, and yacon leaves extract.

Specifically,

Black garlic extract 15 wt%;

Lolium root extract 15 wt%;

Seaweed extract 15 wt%;

Motherwort extract 15 wt%;

Gugija extract 10 wt%;

10% by weight;

And 20 wt% of yacht leaf extract to prepare a wrinkle-improving cosmetic composition according to the present invention.

In the present invention, in order to further improve the wrinkle-improving function of the cosmetic composition,

0.01 to 70 wt% of black garlic extract, 0.01 to 70 wt% of root extract, 0.01 to 70 wt% of seaweed extract, 0.01 to 70 wt% of motherwort extract, 0.01 to 70 wt% of gugija extract, 0.01 to 70 wt% The cosmetic composition for improving basic wrinkles, which is composed of a mixture of 0.01 to 70 wt%

A first mixture composed of a mixture of 0.1 to 50 wt% of a flaxseed extract, 0.1 to 50 wt% of a grape seed extract, 0.1 to 50 wt% of a waxy extract, 0.1 to 50 wt% of a pomegranate seed extract and 0.1 to 50 wt% To thereby form a wrinkle-improving cosmetic composition.

At this time, the amount of the first mixture to be added is 0.01 to 10 wt% based on the total weight of the basic wrinkle-improving cosmetic composition.

<Flaxseed extract>

The flaxseed is Linum usitatissimum and is a seed of the flax (Linaceae) plant. Flaxseed contains vitamins and essential amino acids. However, there are three representative beneficial ingredients that are rich in omega-3 fatty acids, lignans and dietary fiber.

Lignans have antioxidant properties and have very useful antioxidant activities in human body. It has excellent anticancer, anti-cleavage, and anti-toxicity and has a function of strongly inhibiting already formed tumors.

The flaxseed extract is prepared by mixing the flaxseed powder with ethanol at a weight ratio of 1:10 to 15 and then heating and extracting the mixture for 3 to 5 hours. The extract is repeated three times and filtered. The filtrate is filtered at 58 to 63 ° C, 0.1 to 0.3 mPa Under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator.

Specific examples of the flaxseed extract include,

After drying, 1,000 g of the pulverized flaxseed powder is immersed in 10 L of 95% ethanol, and then heated at 62 DEG C for 3 hours. The extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Grape seed extract>

Vitis vinifera Linne is rich in resveratrol, which is known as an antioxidant and phenolic compounds such as anthocyanins, benzoic acid, flavonol, procyanidin, and prophylgallate, as well as organic acids, vitamins and minerals to lower blood cholesterol levels, , Stroke, prevention and treatment of heart disease, anticancer, antioxidant, antifungal action.

Grape seeds contain polyphenol, an antioxidant that converts free radicals in our bodies into harmless substances, inhibits platelet aggregation in the body, prevents cardiovascular disease, and affects blood circulation and lipid metabolism. It contains resveratrol, linolenic acid, vitamin E, phenolic compounds, and sterols.

The grape seed extract is prepared by mixing grape seed powder and distilled water at a weight ratio of 1: 5 to 30, heating the mixture at 100 ° C for 3 to 5 hours, filtering the obtained extract, and filtering the filtrate at a temperature of 58 to 63 ° C under 0.1 to 0.3 mPa And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

More specifically, 1,000 g of the grape seed powder obtained by shaking the grape seed with a homogenizer was added to 10 L of distilled water, and the mixture was heated and extracted at 100 캜 for 4 hours. The obtained extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Wassong extract>

Wa-song (Orostachys japonicus A. Berger) is a perennial plant of the Crassulaceae that grows on the rocks of the old tile roofs or deep mountains.

It is called "warsong" or "rocksol". It has been used for medicinal purposes since it has been used for medicinal purposes because it has been taken from summer to fall, and has been used for medicinal purposes since it has been used for medicinal purposes. It has been used as a folk remedy for a long time in Korea, and has been used for immunity, hemostatic and cancer treatment for hepatitis, Sterol, triterpenoids, flavonoids, and phenolic compounds have been isolated as phytochemical compounds, and these isolates are known to be effective against cancers of digestive system.

In the present study, we investigated the effects of 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, and tannin, gallic acid, and glutinol, And flavonoids such as kaempferol and quercetin have been reported.

The wort extract is prepared by mixing the wort and distilled water at a weight ratio of 1: 5 to 30, refluxing and cooling extraction at 100 ° C for 4 to 7 hours, filtering the obtained extract, and filtering the filtrate under conditions of 58 to 63 ° C and 0.1 to 0.3 mPa And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

More specifically, 1,000 g of raw wort was added to 10 L of distilled water, followed by reflux cooling extraction at 100 캜 for 6 hours. The obtained extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at a temperature of 62 ° C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Pomegranate Seed Extract>

Pomegranate (Punica granatum L.) has been used in a variety of applications such as tonic action, dressing action, tooth decongestion, diarrhea, asthma, whooping cough, and athlete's foot.

The major components of pomegranate are piperidine alkaloids such as isopelletierine, pseudopelletierine, methyl pelletierine and methyl isopelletierine. The total alkaloid content of muscle is 0.6%, the stem shell is 0.5%, and the branch shell is 0.4%. Pomegranate rind contains tannin (terchebin, granatin B. punicacortein A ~ D, casuarinin, 2-O-galloypunicalin), sugars, resins, mannitol, inulin, succinic acid, oxalic acid, pectin, Ca and isoquercetin. -cytosterol, mannitol, seeds contain punic acid, bark manitol, and sorbitol.

The pomegranate seed extract is prepared by mixing pomegranate seed powder with distilled water at a weight ratio of 1: 5 to 30, refluxing and cooling at 100 ° C for 1 to 3 hours, filtering the resulting extract, mPA under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator.

More specifically, 1,000 g of pomegranate seed powder crushed with a homogenizer was added to 10 L of distilled water, followed by reflux cooling and extraction at 100 DEG C for 6 hours. The obtained extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at a temperature of 62 ° C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Extract of turmeric>

Oenanthejavanica is a perennial herbaceous perennial plant belonging to the Umbelliferae.

It has also been used in various folk remedies. It is called Sookeun in Korean medicine. It has fever and diuretic effect and it is used to treat jaundice, water, urine discomfort and hypertension.

Among the minerals, the content of potassium is high, and 392 mg per 100 g is contained. 1320 μg of β-carotene and 15 mg of vitamin C are contained. In addition, the effects of anticancer effects and antimutagenic effects have also been reported in other studies on hemp cultures, including neuroprotective effects, inhibition of angiotensin-I-converting enzyme activity, and toxicity of hepatocytes [37-39] .

The extract of Pyrrhizae radix and ethanol is mixed at a ratio of 1: 5 ~ 30 by weight and extracted at 65 to 75 ° C for 20 to 30 hours. The obtained extract is filtered and the filtrate is filtered at 58 to 63 ° C under 0.1 to 0.3 mPa And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

More specifically, 1,000 g of Piglets are added to 70% ethanol and extracted at 72 캜 for 26 hours. The obtained extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at a temperature of 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The wrinkle improving cosmetic composition according to Example 2 was prepared by adding a first mixture composed of a mixture of flaxseed extract, grape seed extract, wanshom extract, pomegranate extract, and fenugreek extract to a foundation wrinkle improving cosmetic composition .

Specifically,

Black garlic extract 13 wt%;

Lantus root extract 13 wt%;

Seaweed extract 13 wt%;

Motherwort extract 13 wt%;

Gugija extract 10 wt%;

10% by weight;

Yacht leaf extract 20 wt%;

8 wt% of a first mixture of 100 wt% composed of 20 wt% of flaxseed extract, 20 wt% of grape seed extract, 20 wt% of wanshang extract, 20 wt% of pomegranate seed extract and 20 wt% of fennel extract Thereby forming a cosmetic composition for improving wrinkles according to the present invention.

In the present invention, in order to further improve the wrinkle-improving function of the cosmetic composition,

0.01 to 70 wt% of black garlic extract, 0.01 to 70 wt% of root extract, 0.01 to 70 wt% of seaweed extract, 0.01 to 70 wt% of motherwort extract, 0.01 to 70 wt% of gugija extract, 0.01 to 70 wt% The cosmetic composition for improving basic wrinkles, which is composed of a mixture of 0.01 to 70 wt%

A second mixture composed of a mixture of 0.1 to 50 wt% of an oat extract, 0.1 to 50 wt% of a spirulina extract, 0.1 to 50 wt% of a fermented red ginseng extract, 0.1 to 50 wt% of an upper leaf extract and 0.1 to 50 wt% To thereby form a wrinkle-improving cosmetic composition.

At this time, the amount of the second mixture to be added is 0.01 to 10 wt% based on the total weight of the base wrinkle improving cosmetic composition.

<Oylum extract>

Oufa (Sanguisorba officinalis L.) is a perennial plant belonging to the Rosaceae (Rosaceae). It is a perennial plant commonly found in the mountains and fields of the country.

The main components of Olefum include saponin glycosides containing acid guisorbin and porcelain, pomolic acid, sanguisorbic acid, kaempferol, gallic acid, ellagic acid, catechin, etc. , And the outposts and leaves contain components such as tannins, flavonoids, quinone compounds, and vitamin A.

The oat extract is prepared by mixing oat toppings and ethanol at a weight ratio of 1: 5 to 30, shaking at 65 to 75 ° C at 100 to 120 rpm for 2 to 6 hours, filtering the obtained extract, Under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator

More specifically, 1,000 g of the dried ovicle extrudate was added to 10 L of 50% ethanol, and the mixture was extracted at 70 DEG C and 110 rpm for 5 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Spirulina extract>

Spirulina (Spirulinaplatensis) is one of the oldest algae on Earth and is the first photosynthetic creature born about 3.5 billion years ago.

Ingredients include 60-70% protein, 6-9% lipid, carbohydrate 15-20% carbohydrate and large amounts of vitamins, minerals and fiber. Among the lipid components, free-fatty acid accounts for 70 ~ 80%, which is composed of linoleic acid and γ-linolenic acid.

Carbohydrates include glucose, rhamnose, mannose, and xylose. Coloring agents include light yellow carotenoids, green chlorophyll, and blue phycocyanin. It also contains a large amount of vitamin B12 and phenolic acid, tocopherols, and β-carotene, which act as antioxidants.

The spirulina extract is prepared by mixing spirulina and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours, and extracting the obtained extract in a centrifuge at 7,000 to 9,000 rpm for 10 to 30 minutes. The supernatant is then filtered, and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPA.

More specifically, 1,000 g of Spirulina was added to 25 L of distilled water, and the mixture was shaken for extraction at 25 DEG C and 150 rpm in an incubator for 70 hours. The extracted extract is centrifuged at 8,500 rpm for 20 minutes in a centrifuge. The supernatant was collected by filtration through filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Fermented red ginseng extract>

Ginseng has been reported to be one of the most prevalent medicinal herbs since ancient times, and has been reported to have the effects of promoting metabolism, detoxifying toxins, protecting liver, promoting liver regeneration, promoting alcoholysis, improving hyperlipidemia, improving diabetes and improving hypertension have.

In the red ginseng prepared by steamed and dried ginseng, soluble saponins and caffeic acid, saicylic acid, ferulic acids, vanillic acid, maltol, panaxynol, diol and triol saponin belonging to phenolic acids, ginsenoside Rb1 and Rb2, matol (2-methy- 3,3-hydroxypyrone), cephaloridine, panaxadiol, panaxatriol, etc., have been shown to enhance antioxidant activity and antioxidant activity against lipid peroxidation in liver tissues and to protect against hepatotoxicity induced by carbon tetrachloride and galactosamine .

The fermented red ginseng extract is prepared by mixing the fermented red ginseng and water at a weight ratio of 1: 5 to 30 and repeating the process of extracting at 80 to 93 ° C for 5 to 10 hours for 2 to 4 times, extracting the extract, filtering the obtained extract The filtrate is concentrated under reduced pressure using a vacuum rotation evaporator at 0.1 to 0.3 mPA for 5 to 7 hours.

More specifically, fresh ginseng is washed, sun-dried, and then boiled at 92 ° C for 4 hours. After the addition of fresh ginseng, Paecilomyces variotii var. fermented in brunneolus culture at 32 ° C for 50 hours. Fermented red ginseng is dried at 55 ℃ for 15 hours to produce fermented red ginseng.

1,000 g of the fermented red ginseng is added to 5 L of distilled water and extracted at 100 ° C for 7 hours. All three extracts were collected by filtration through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA with a vacuum rotation evaporator for 5 hours to obtain 1 L of an extract.

&Lt;

Mulberry leaves are called mulberry leaf (Mori Folium) and have been used for silkworms since ancient times. Recently, however, various functions have been known and used as a car. The upper leaves are rich in protein, vitamins, minerals and dietary fiber, and contain various physiologically active substances such as flavones, steroids and triterpenes.

The upper leaf extract is prepared by mixing the upper leaves with ethanol at a weight ratio of 1: 5 to 30, shaking at 65 to 75 ° C at 100 to 120 rpm for 3 to 7 hours, filtering the resulting extract, centrifuging at 4,000 to 6,000 rpm After separation, a supernatant is obtained, and the supernatant is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA

More specifically, 1,000 g of the top leaves are added to 10 L of 70% ethanol and extracted at 70 DEG C and 110 rpm for 5 hours. The obtained extract was filtered with a filter paper (Whatman No. 2), and the filtrate was centrifuged at 5,000 rpm for 40 minutes in a centrifuge to obtain a supernatant. The supernatant was concentrated under reduced pressure at 0.2 mPA for 5 hours with a vacuum rotation evaporator to obtain 1 L of an extract.

<Safflower extract>

Safflower is a one-year-old herb that belongs to the Department of Asteraceae (Compositae), also known as red flame and safflower.

The safflower extract is prepared by mixing safflower and ethanol in a weight ratio of 1: 5 to 30, extracting the mixture at 60 to 100 ° C for 2 to 7 hours, filtering the resulting extract, filtering the filtrate at a concentration of 0.1 to 0.3 mPa for 5 to 7 hours The product is concentrated under reduced pressure using a vacuum rotation evaporator.

More specifically, 1,000 g of safflower is added to 10 L of 95% ethanol and then extracted in a heating mantle at 80 DEG C for 4 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The wrinkle improving cosmetic composition according to Example 3 was further blended with a base wrinkle improving cosmetic composition in such a manner that a second mixture composed of an oat extract, Spirulina extract, fermented red ginseng extract, mulberry leaf extract, .

Specifically,

Black garlic extract 13 wt%;

Lantus root extract 13 wt%;

Seaweed extract 13 wt%;

Motherwort extract 13 wt%;

Gugija extract 10 wt%;

10% by weight;

Yacht leaf extract 20 wt%;

8 wt% of a second mixture of 100 wt% composed of 20 wt% of oat extract, 20 wt% of spirulina extract, 20 wt% of fermented red ginseng extract, 20 wt% of leaf extract and 20 wt% of safflower extract Thereby forming a cosmetic composition for improving wrinkles according to the present invention.

In the present invention, in order to further improve the wrinkle-improving function of the cosmetic composition,

0.01 to 70 wt% of black garlic extract, 0.01 to 70 wt% of root extract, 0.01 to 70 wt% of seaweed extract, 0.01 to 70 wt% of motherwort extract, 0.01 to 70 wt% of gugija extract, 0.01 to 70 wt% The cosmetic composition for improving basic wrinkles, which is composed of a mixture of 0.01 to 70 wt%

A third mixture consisting of a mixture of bokbunja extract 0.1 to 50 wt%, chewing gum extract 0.1 to 50 wt%, dodok extract 0.1 to 50 wt%, ginseng extract 0.1 to 50 wt%, and peony extract 0.1 to 50 wt% Thereby forming a cosmetic composition for improving wrinkles.

At this time, the amount of the third mixture to be added is 0.01 to 10 wt% with respect to the total weight of the base wrinkle improving cosmetic composition.

<Bokbunja extract>

Rubus coreanus Miq. Is a deciduous shrub of rosacea, which refers to the fruit of bokbunja strawberry as medicinal material. Quercetin, sanguinin H-5, ellagic acid, 3-O-β-D-galactose, and phenol compounds are the main nutrients. glucuronide have been reported.

The brambly extract is prepared by mixing brambles powder with methanol at a weight ratio of 1: 5 to 30 and extracting the mixture at 75 to 85 ° C for 1 to 3 hours. The extracted extract is filtered for 5 to 7 hours at 0.1 to 0.3 mPa The product is concentrated under reduced pressure using a vacuum rotation evaporator.

More specifically, 1,000 g of the powdery brambled powder obtained by pulverizing dried brambles was added to 7 L of 70% methanol, and the mixture was extracted at 80 DEG C for 1 hour. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Chunma extract>

Gastrodia elata Blume is a member of the Orchidaceae family. It is a plant that grows nourishment from Armillaria Mellea, which is inoculated on rotten roots such as oak, without being nourished by itself. Chromosomes are known to be effective not only for stress and fatigue but also for diseases such as headache, paralysis, cranial nervousness, hypertension and diabetes.

These effects have long been used as medicines in oriental medicine. These effects are known to be due to components such as vanillin, 4-hydroxybenzaldehyde and the like, which are reported to be effective ingredients in chewing gum. It has also been reported that ergothioneine and diosgenin present in mushroom as a powerful antioxidant component are also present in chimaese.

The chewing gum extract was prepared by mixing a mixture of chewing gum and distilled water at a weight ratio of 1: 5 to 30, extracting the mixture at 85 to 95 ° C for 2 to 5 hours using a reflux extractor, and extracting the extract at 4,000 to 5,000 rpm at 3 to 10 ° C Separate the supernatant using a centrifuge, filter the supernatant, filter the remaining precipitate, and concentrate under reduced pressure for 5 to 7 hours at 0.1 to 0.3 mPA with a vacuum rotation evaporator.

More specifically, 1,000 g of dried chestnut was added to 20 L of distilled water and extracted at 90 DEG C for 3 hours using a reflux extractor. The extract is centrifuged at 4,500 rpm and 8 ° C for 15 minutes using a centrifuge. The supernatant was separated, filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours at 0.2 mPA with a vacuum rotation evaporator to obtain 1 L of an extract.

<Deodorant extract>

Codonopsislanceolata is a perennial vine plant belonging to the lantern family and its roots are called radixCodonopsislanceolatae.

Dodec is a carbohydrate, protein, calcium, phosphorus, iron, vitamin B, oleanolic acid, albigenic acid, phytosterol, saponin, inulin, flavonoid, sterol, triterepenoid, cycloartenol, N-formylharman, perloyrine, norharman, 1-carbomethoxy-β-carboline , apinasterol, squalene, triterpenoid, α-spinasterol, β-spinasterol, oleanolic acid, alligenic acid, apigenin, vitamineB1, B2, inulin, triterpene, steroid, flavonoid and volatile flavor components

Dodec, which contains these ingredients, inhibits pediatric bacterium, human type and small tubercle bacillus by strengthening gastrointestinal function, constipation, strengthening of heart function, cholesterol decomposition, blood pressure reduction, vasodilation, blood purification, anti- , Lung cancer, and thyroid cancer.

The dodok extract was prepared by mixing doduk and methanol in a weight ratio of 1:20 to 22, adding the mixture to a reflux extractor, supplying cooling water to a condenser at the upper end of the extractor, and repeating 3 times at 58 to 62 ° C for 3 hours to obtain an extract And the extract is filtered in a vacuum filtration apparatus and concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

Specific examples of the roe extract include,

After mixing 1,000 g of finely divided dodeca with 20 L of methanol, the mixture is added to a reflux extractor, and cooling water is supplied to the condenser at the upper end of the extractor, and extracted three times at 60 ° C for 3 hours. The extract is filtered using a filter paper (Whatman filter paper (No. 2)). The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Gossam Extract>

Gosam (Sophora falvescens Aiton) belongs to the bean family and is also called the nursery, the staff of the thief. It has been used in diabetes, dysentery, fever, eczema, gastrointestinal bleeding and antimicrobial action in the oriental medicine. It is clinically known to be externally used for eczema, skin pyoderma and skin diseases such as female genital warts and also for bacterial dysentery and enteritis .

The components of gosam known to date include alkaloids, flavonoids, saponins, etc., and contain 1 ~ 2% of alkaloids and 0.5% of flavonoids as main ingredients.

The extract was obtained by filtering the extracts at 68 to 72 ° C for 22 to 26 hours, filtering the extracts in a vacuum filtration apparatus, and then washing the extracts with 0.1 to 0.3 mPA Under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours.

Specific examples of the gosam extract include,

1,000 g of the raw body of fresh ginseng is added with 10 L of 90% ethanol, extracted at 70 ° C for 24 hours, and filtered through a reduced pressure filtration apparatus. The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Peony extract>

Peony (Paeonia lactiflora Pall.) Belongs to the family Ranunculaceae. Several of the stems come out of the root and stand straight. The leaves and stems have no hairs and the roots are thick. It is mainly used as a medicinal herb, and its main effects and effects are analgesic, antipyretic, antipyretic, diuretic, laxative, abdominal pain, stomach pain, arthritis, anemia and bruises.

Studies on physiological activity against peony have revealed anti - inflammatory, anti - allergic analgesic, antimicrobial activity and whitening effect. Paeoniflorin, Paeonol, Albiflorin, etc.

The peony extract was prepared by mixing peony powder and ethanol at a weight ratio of 1:10 to 12 and then dipping the peel solution at room temperature for 22 to 26 hours and separating and filtering the supernatant by repeating the procedure three times. And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

Specific examples of the peony root extract include,

1,000 g of peony pellets are mixed with 10 L of 90% ethanol, then immersed at room temperature for 26 hours, and the supernatant is separated and filtered repeatedly three times. The filtrate was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The wrinkle improving cosmetic composition according to Example 4 was further added to the base wrinkle improving cosmetic composition in order to further improve the wrinkle improving function, the third mixture formed by mixing the bokbunja extract, the gmma extract, the dodok extract, the gossam extract and the peony extract .

Specifically,

Black garlic extract 13 wt%;

Lantus root extract 13 wt%;

Seaweed extract 13 wt%;

Motherwort extract 13 wt%;

Gugija extract 10 wt%;

10% by weight;

Yacht leaf extract 20 wt%;

8 wt% of a third mixture of 100 wt% composed of 20 wt% of bokbunja extract, 20 wt% of cheongma extract, 20 wt% of rosemary extract, 20 wt% of rosemary extract and 20 wt% of rosemary extract was mixed, To prepare a cosmetic composition for improving wrinkles.

In the present invention, in order to further improve the wrinkle-improving function of the cosmetic composition,

0.01 to 70 wt% of black garlic extract, 0.01 to 70 wt% of root extract, 0.01 to 70 wt% of seaweed extract, 0.01 to 70 wt% of motherwort extract, 0.01 to 70 wt% of gugija extract, 0.01 to 70 wt% The cosmetic composition for improving basic wrinkles, which is composed of a mixture of 0.01 to 70 wt%

The fourth mixture was prepared by mixing 0.1 to 50 wt% of rice bran extract, 0.1 to 50 wt% of guinea pig extract, 0.1 to 50 wt% of white pine bark extract, 0.1 to 50 wt% of mushroom extract and 0.1 to 50 wt% To further form a wrinkle-improving cosmetic composition.

At this time, the addition amount of the fourth mixture is 0.01 to 10 wt% based on the total weight of the base wrinkle improving cosmetic composition.

<Rice bran extract>

It is called brown rice in which rice husk is threshed and rice husk which is outer surface is peeled off, and brown rice is made and rice husk is removed. Rice bran is rice flour made of rice husks and rice bran, which is produced in the process of turning from brown rice to white rice. It accounts for about 8% of brown rice.

Rice cores are mainly composed of fat, protein, and dietary fiber. Vitamin A, vitamin B such as thiamine, pyridoxine, and niacin, and minerals such as calcium, zinc and iron.

The extract of the rice bran was mixed with ground rice bran and ethanol at a weight ratio of 1:10 to 15, and the mixture was heated and extracted for 3 to 5 hours. The extracted extract was filtered three times and the filtrate was filtered at 58 to 63 ° C And concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours.

Specific examples of the above rice bran extract include,

1,000 g of the ground rice bran was added to 95% ethanol, and the mixture was heated at 62 ° C for 4 hours for extraction. The extract obtained by repeating this operation three times was filtered with filter paper (Whatman Filter paper No. 2, USA), and the filtrate was concentrated under reduced pressure at 62 캜 and 0.2 mPA for 5 hours by a vacuum rotation evaporator 1 L of an extract is obtained.

<Ingredients>

Caesalpiniasappan L. is a deciduous shrub legume plant distributed in tropical Asia, including India, Malaysia and southern China.

The physiological activity studies on the extracts of jojoba include analgesic, antiinflammatory, bloodtonic, expectorant, emmenagogue, spasmolyticactivity, anticonvulsant, The compounds of the present invention are useful for the treatment of inflammatory diseases such as spasmolyticactivity, vasorelaxation, antiatherogenic, anticomplementary, immunomodulation, induction ofimmunomodulation, antimicrobial, cytotoxic, Antiviral, hypoglycemic, and antioxidation have been reported.

The crude extract was prepared by mixing crude extract and ethanol in a weight ratio of 1: 5 to 10, leaching at room temperature for 24 hours, filtering the primary extract, repeating the filtration twice in the same manner, And concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours.

Specific examples of the above-

After drying, 1,000 g of the crushed starch is placed in 15 L of 80% ethanol, leached at room temperature for 24 hours, and then the primary extract is filtered. After repeating filtration twice in the same manner, all the extracts were mixed.

The extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<White pine bark extract>

Ringworm (DictamnusdasycarpusTurez) is a perennial herb that belongs to rutaccae and is called Dictamni Radicis Cortex. It is known that phyllotaxin contained dictamnine, limonin, sitosterol, choline, and Fraxinellone. The extract has an inhibitory effect against various pathogens such as yellow pycnogen, symmetric pycnogenina, and pneumoniae.

The white pine bark extract was prepared by mixing white blood and distilled water at a weight ratio of 1:15 to 20, heating and extracting at 100 ° C for 2 to 3 hours, centrifuging at 2,800 to 3,200 rpm for 10 to 20 minutes to remove debris, , The supernatant is filtered in a vacuum filtration apparatus and concentrated under reduced pressure using a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.

Specific examples of the above-

1,000 g of white radish is mixed with 1.5 L of distilled water, shaken at 100 ° C. for 2 hours, and then centrifuged at 3,000 rpm for 15 minutes to remove debris to obtain a supernatant. The supernatant was filtered through a filtration apparatus under reduced pressure, and the filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Situ mushroom extract>

Mushrooms contain polysaccharides, proteins and fats as well as tocopherols with vitamin E activity and beta-carotene, which is effective for antioxidant activity. One of the ingredients, carotenoids, is known as Vitamin A, which helps vision and antioxidant activity. It also contains components such as lectin, cresustine, and lectin, which are effective for anti-cancer effects.

The mushroom extract is prepared by mixing the mushroom with ethanol at a weight ratio of 1: 5 to 30, extracting the mixture at 120 to 180 ° C for 3 to 7 hours, filtering the obtained extract, And concentrated under reduced pressure using a vacuum rotation evaporator.

More specifically, 1,000 g of Phellinus linteus powder was added to 10 L of 95% ethanol and extracted in a heating mantle at 160 DEG C for 4 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Yeast extract>

Yeast (Momordica charantia L., bitter melon) is a member of the annual Tropical Cave (Cucurnitaceae) in Asia, the Amazon, East Africa and the Caribbean.

Yeoju contains a large amount of vitamin A, vitamin C and iron, and is marketed in Japan as a variety of teas, drinks, and health functional foods. Studies on the physiological activity of Yeoju have been reported on the antidiabetic effect of Yeoju extract and powder, and the antioxidant activity of Yeoju on the variety of Yeoju.

The extracts of Yeoju were prepared by mixing Yeoju and ethanol at a weight ratio of 1:10 to 15, and then repeatedly extracted three times for 22 to 26 hours at room temperature. The extracted extract was filtered, and the filtrate was vacuum dried at 58 to 63 ° C for 5 to 7 hours And concentrated under reduced pressure using a vacuum rotation evaporator.

As a specific example,

1,000 g of Yeoju is immersed in 10 L of 95% ethanol and then extracted three times at room temperature for 24 hours. The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The wrinkle-improving cosmetic composition according to Example 5 was prepared by mixing a fourth mixture composed of a rice bran extract, a root extract, a white roots extract, a mushroom extract, and a Yeoju extract in a base wrinkle improving cosmetic composition .

Specifically,

Black garlic extract 13 wt%;

Lantus root extract 13 wt%;

Seaweed extract 13 wt%;

Motherwort extract 13 wt%;

Gugija extract 10 wt%;

10% by weight;

Yacht leaf extract 20 wt%;

8 wt% of a fourth mixture of 100 wt% composed of a mixture of 20 wt% of rice bran extract, 20 wt% of root extract, 20 wt% of white pine bark extract, 20 wt% of mushroom extract and 20 wt% Thereby producing a wrinkle-improving cosmetic composition according to the present invention.

In the present invention, in order to extend the preservation period of the wrinkle-improving cosmetic composition,

0.01 to 70 wt% of black garlic extract, 0.01 to 70 wt% of root extract, 0.01 to 70 wt% of seaweed extract, 0.01 to 70 wt% of motherwort extract, 0.01 to 70 wt% of gugija extract, 0.01 to 70 wt% The cosmetic composition for improving basic wrinkles, which is composed of a mixture of 0.01 to 70 wt%

A fifth mixture composed of a mixture of 0.1 to 50 wt% of an acidic extract, 0.1 to 50 wt% of a moss extract, 0.1 to 50 wt% of an extract of Ganoderma lucidum, and 0.1 to 50 wt% of a grapefruit seed extract is further added to prepare a cosmetic composition.

At this time, the amount of the fifth mixture is added in an amount of 0.01 to 3 wt% based on the total weight of the wrinkle-improving cosmetic composition.

<Sancho extract>

Zanthoxylum schinfolium (Zanthoxylum schinfolium) is a species native to a mountainous area under 1,000m above sea level in Korea. Its fruit is used as vegetable oil resources and various seasonings, and seed oil is also used as a lubricant for millet.

Sancho is a fruit of Yeoju. It contains 2 ~ 4% of essential oil in the fruit husk and its main components are dipentene (dl-limonene), citronellal, ℓ-β-phellandren, geraniol, . The fruit contains xanthoxine, a phlegmatic toxin, xanthoxine acid, a paralytic component, sterol campesterol, stigmasterol, and β-sitosterol.

The mixture was mixed at a weight ratio of 1: 5 to 30: 1, and the mixture was stirred at room temperature for 22 to 26 hours. The resulting extract was filtered, and the filtrate was filtered through a vacuum rotation evaporator To 0.3 mPA under reduced pressure for 5 to 7 hours.

More specifically, 1,000 g of anthracnose and 10 L of 80% methanol are mixed and extracted with stirring at 25 DEG C for 24 hours. The extract was filtered with filter paper No. 2 and concentrated under reduced pressure at 62 ° C and 0.2 mPA using a vacuum rotation evaporator for 5 hours to obtain 1 L of an extract.

<Moss extract>

Moss has been reported to have excellent antimicrobial and antioxidant effects. When mosses are added to water, the acidity of the surrounding water is increased by the object of mosses in the body, thereby reducing the bacterial activity and preventing the deterioration of water.

There are lichen (moss, moss, Musci), umbrella (liverwort, Hepaticae), and horny lichen (hornwort, Anthocerotae). These lichens inhibit microbial propagation and are highly valuable as natural preservatives because of their antioxidant action.

The moss extract was washed with hot water at 55 to 65 ° C and then dried by hot air blowing. The moss was mixed with 100% methanol at a weight ratio of 1: 5 to 30, and then cooled in a reflux condenser equipped with a cooling tube. The mixture is refluxed and cooled at 62 ° C for 5 to 7 hours, the resulting extract is filtered, and the filtrate is concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator.

More specifically, a moss mixture obtained by mixing moss, large feather moss, and feather moss at the same weight ratio is washed with water and dehydrated and then dried by hot air drying at 62 DEG C for 6 hours. 1,000 g of the pulverized dried sample and 10 L of 100% methanol are mixed in a reflux condenser equipped with a cooling tube and reflux cooled for extraction at 58 ° C. for 7 hours.

The extract was filtered with a pore size filter paper having a pore size of 5 μm and concentrated under reduced pressure at 62 ° C. and 0.2 mPA using a vacuum rotation evaporator for 5 hours to obtain 1 L of an extract.

<Baekhyon extract>

It is a perennial plant belonging to the ranunculaceae family. It is distributed in all parts of Korea and also in northeast China. This herbal medicine has been commonly applied to toothache, fever, and water, and has been used for anti-inflammation, astringency, hemostasis, dysentery, detoxification, scrotum, antibacterial, sedation, and anticancer. It has been reported that this material has been widely used for anti-inflammation, analgesic, antipyretic, and detoxification purposes in Korea.

The Baekguyong extract was washed with water, dried in a freeze dryer, processed into a powder form, powdered Baekjonggwa and distilled water were mixed in a weight ratio of 1: 5 to 15, and the mixture was heated at 100 ° C for 2 to 4 hours under reflux , And the extract is filtered with a filter paper, and the filtered extract is concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator.

As a concrete example of the extract, the powder is prepared by mixing 1,000 g of powdered hymenophorone with 10 L of distilled water to prepare a mixture, and then the mixture is reflux-extracted at 100 ° C for 3 hours. Filter the extract with filter paper. The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Grapefruit seed extract>

Grapefruit is juicy and has a bit of sour taste. Grapefruit seed extract is a natural antimicrobial material, and its main components are ascorbic acid, palmitic acid, glucoside, and naringin glycocortoferol, which inhibit mycotoxin biosynthesis and growth of fungal strains.

The grapefruit seed extract is prepared by mixing grapefruit seed and ethanol at a weight ratio of 1: 5 to 30, refluxing the mixture at a temperature of 60 to 62 ° C for 2 to 3 hours, centrifuging at 10,000 × g for 20 to 40 minutes, The filtered extract is concentrated under reduced pressure for 5 to 7 hours at 0.1 to 0.3 mPA using a vacuum rotation evaporator.

As a specific example of the grapefruit seed extract, 1,000 g of grapefruit seed is added to 10 L of 50% ethanol, and the mixture is refluxed for 3 hours at 60 ° C for extraction. The extracted extract is centrifuged at 10,000 xg for 30 minutes. Take the supernatant and filter it through filter paper. The filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The fifth mixture was tested for antiseptic activity against the bacterial strains Staphylococcus aureus KCCM 11335, Escherichia coli, Pseudomonas aeruginosa KCCM 11802, Candida albicans KCCM 11282 and Aspagillus niger KCCM 60143 which are problematic in cosmetics.

E. coli, KCCM 40271), Candida albicans (C. albicans KCCM 11282), fungi (E. coli KCCM 11282), and yeast strains of Staphylococcus aureus (S. aureus, KCCM 11335), Pseudomonas aeroginosa (P. aeroginosa, KCCM 11802) and Escherichia coli Aspergillus niger (A. niger KCCM 60143) was cultured, and the bacteria were cultured in a shaking incubator adjusted to 37 ° C and fungi at 26 ° C and 250 rpm.

The number of viable cells was measured on days 1, 3 and 7 after inoculation with the bacterial strain thus cultured. As a result, no viable cells were detected.

The wrinkle-improving cosmetic composition according to Example 6 was further added to the base wrinkle-improving cosmetic composition to further improve the wrinkle-improving function, and a fifth mixture composed of a mixture of an acidulant extract, a moss extract, a mung bean extract, do.

Specifically,

Black garlic extract 15 wt%;

Lolium root extract 15 wt%;

Seaweed extract 14 wt%;

Motherwort extract 14 wt%;

Gugija extract 10 wt%;

10% by weight;

Yacht leaf extract 20 wt%;

2 wt% of a fifth mixture of 100 wt% of a mixture of 25 wt% of a sancho extract, 25 wt% of a moss extract, 25 wt% of an extract of Baekduong, and 25 wt% of a grapefruit seed extract was mixed with the wrinkle- To form a composition.

Hereinafter, production examples of wrinkle-improving cosmetics using the wrinkle-improving cosmetic composition shown in Examples 1 to 6 will be described with reference to Examples 7 to 12.

1. A phase composed of 510.6 g of distilled water, 150.0 g of glycerine and 0.1 g of Ethylenediaminetetraacetic Acid (EDTA-2Na) is completely dissolved by heating at 72 ° C.

2. Add 80.0 grams of Cyclomethicone (DC345), 50.0 grams of Cetearyl alcohol, 30.0 grams of Mineral oil, 30.0 grams of Grape seed oil, 30 grams of Cetearyl Glucoside, glucoside, 20.0 g of sorbitan sesquioleate, 15.0 g of polyoxyethylene alkyl ether, 10.0 g of isopropyl myristate, 10.0 g of panthenol, 10.0 g of Glyceryl stearate / PEG-300 stearate, 2.0 g of Dimethicone and 0.3 g of Butylated Hydroxy Toluene (BHT) The B phase is completely dissolved by heating at 72 ° C.

3. Add the completely dissolved B phase to the completely dissolved A phase and stir at 2,800 rpm for 15 minutes to form an emulsion.

4. Xanthan gum (2.0 g) and the cosmetic composition of Example 1 (50.0 g) were mixed at 25 占 폚 to prepare a C-phase.

5. Add the C phase to the emulsion and stir at 4,000 rpm for 10 minutes to complete the wrinkle-improving cream.

In this Example, a cream is prepared through the same procedure as in Example 7 except that 50.0 g of the cosmetic composition of Example 2 is used in the case of using the cosmetic composition.

In this Example, a cream is prepared through the same procedure as in Example 7, except that 50.0 g of the cosmetic composition of Example 3 is used in the case of using the cosmetic composition.

In this Example, a cream is prepared through the same procedure as in Example 7, except that 50.0 g of the cosmetic composition of Example 3 is used in the case of using the cosmetic composition.

In this Example, a cream is prepared through the same procedure as in Example 7, except that 50.0 g of the cosmetic composition of Example 4 is used in the case of using the cosmetic composition.

In this Example, a cream is prepared in the same manner as in Example 7, except that 50.0 g of the cosmetic composition of Example 5 is used in the case of using the cosmetic composition.

[ Test Example 1 ]

1. Elastase inhibition rate measurement

Elastase and N-succinyl- (Ala) _3- p-nitroanilide increased the activity. The degree of inhibition was determined by IC ₅₀ (㎍ / ㎖).

Substrate A 200 μl of 1.0 mM N-succinyl- (Ala) ₃ -p-nitroanilide was mixed with 20 μl of a sample solution diluted with 500 μg / ml of each of the wrinkle-improving cosmetic compositions of Examples 1 to 5 and 10 μl of 2.5 U Elastase After incubation at 25 ° C for 10 min, absorbance was measured at 410 nm with an ELISA reader. Elastase inhibition rate is expressed as the absorbance reduction rate of the addition of the sample solution and the non-addition solution as follows.

Inhibition rate (%) = {1- (absorbance of sample added / absorbance of non-additive)} x 100

The inhibition rate of Example 1 was 52.41%, the inhibition rate of Example 2 was 56.24%, the inhibition rate of Example 3 was 57.78%, the inhibition rate of Example 4 was 50.04% 56.98%, and the inhibition rate of Example 5 was 57.55%.

2. Measurement of collagenase inhibition rate

The degree of inhibition was measured by IC ₅₀ (㎍ / ㎖) at 50% reduction in activity by evaluating the activity of collagenase degrading collagen.

Collagen is dissolved in 20 mM Tris-HCl (pH 7.5) and 5 mM CaCl ₂ buffer solution to make 3 μg / ml of substrate solution. 200 μl of Buffer, 20 μl of the substrate solution, 20 μl of the sample solution diluted to 500 μg / ml of each of the wrinkle-improving cosmetic compositions of Examples 1 to 5, and the mixture was reacted at 37 ° C. for 1 hour with addition of collagenase. 100 μl of 2% ninhydrin reagent was added and reacted for 20 minutes. Absorbance was measured at 570 nm with an ELISA reader.

As a result of the measurement, the inhibition rate in Example 1 was 75.54%, the inhibition rate in Example 2 was 78.12%, the inhibition rate in Example 3 was 81.49%, the inhibition rate in Example 4 was 79.78%, and the inhibition rate in Example 5 was 80.44%.

It was confirmed that the wrinkle-reducing cosmetic composition of Examples 1 to 5 had the wrinkle-reducing effect through the results of the inhibition rate of Elastase and the inhibition rate of collagenase, It is possible to predict the wrinkle-improving effect of cosmetics containing the wrinkle-improving cosmetic composition.

[ Test Example 2 ]

The results of antimicrobial test are presented to confirm the preservation and life extension effect of the cream prepared according to Example 12.

Antimicrobial tests were performed on the strains Staphylococcus aureus KCCM 11335, Escherichia coli, Pseudomonas aeruginosa KCCM 11802, Candida albicans KCCM 11282 and Aspagillus niger KCCM 60143.

E. coli, KCCM 40271), Candida albicans (C. albicans KCCM 11282), fungi (E. coli KCCM 11282), and yeast strains of Staphylococcus aureus (S. aureus, KCCM 11335), Pseudomonas aeroginosa (P. aeroginosa, KCCM 11802) and Escherichia coli Cultured Aspergillus niger (A. niger KCCM 60143) was cultured in a shaking incubator at 37 ° C and fungi at 26 ° C and 250 rpm.

The bacterial strain thus cultured was inoculated into the cream prepared according to Example 12, and the viable cell counts were measured on days 1, 3 and 7, respectively. As a result of measurement, no viable cells were detected.

The wrinkle-improving cosmetic composition using the natural substance according to the present invention and the cosmetic composition containing the natural substance have excellent skin safety by using natural extracts, and are highly industrially applicable because they have excellent wrinkle-removing function using natural extracts.

Claims (7)

The mixture of black garlic and distilled water is mixed at a weight ratio of 1: 5 to 30, and the mixture is shaken at 100 to 140 rpm for 1 to 5 hours. The resulting filtrate is filtered for 5 to 7 hours at 0.1 to 0.3 mPa 0.01 to 70 wt% of black garlic extract concentrated under reduced pressure with a vacuum rotation evaporator;
Dried and mixed with ethanol at a weight ratio of 1: 5 to 30, and then extracted by immersing at room temperature for 20 to 30 hours. The obtained extract was filtered, and the filtered filtrate was concentrated under reduced pressure for 5 to 7 hours under the condition of 0.1 to 0.3 mPa 0.01 to 70 wt% of root rot extract concentrated by vacuum concentrator (vacuum rotation evaporator);
Seaweeds mixed with seaweed, kelp, kelp, and rhubarb were weighed at 58 ~ 62 ℃ for 10 ~ 14 hours at 58 ~ 62 ℃, and the dried powder and distilled water were mixed at 1: 15 ~ 20 weight ratio. 0.01 to 70 wt% of a seaweed extract obtained by concentrating the extract with a vacuum rotation evaporator under a condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure;
The motherwort and distilled water are mixed at a weight ratio of 1: 5 to 30, and the mixture is shaken at 100 ° C and 140 rpm for 1 to 5 hours. The resulting extract is centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes in a centrifuge After separation, the supernatant is filtered, and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator at a concentration of 0.1 to 0.3 mPA for 5 to 7 hours.
The dried gugija and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 100 to 140 rpm for 1 to 5 hours. The resulting extract was centrifuged at 3,000 to 5,000 rpm for 10 to 30 minutes After centrifugation, the supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours at 0.1 to 0.3 mPA.
The mixture of the granular powder and ethanol was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 65 to 75 ° C at 100 to 120 rpm for 2 to 6 hours. The obtained extract was filtered, 0.01 to 70 wt.% Of extracts of geranium bark concentrated under reduced pressure by vacuum rotation evaporator for 7 hours;
The yacon leaf powder and ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 65 to 75 ° C at 100 to 120 rpm for 2 to 6 hours. The obtained extract was filtered and washed with 5 And 0.01 to 70 wt% of a yacon leaf extract concentrated under reduced pressure using a vacuum rotation evaporator for about 7 hours to obtain a wrinkle-improving cosmetic composition using the natural substance.
The method according to claim 1,
The flaxseed powder and ethanol were mixed at a weight ratio of 1:10 to 15, and the mixture was heated and extracted for 3 to 5 hours. The extracted solution was filtered three times and the filtrate was filtered at 58 to 63 ° C under 0.1 to 0.3 mPa 0.1 to 50 wt% of flaxseed extract concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours;
The grape seed powder and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was heated and extracted at 100 DEG C for 3 to 5 hours. The obtained extract was filtered, and the filtrate was filtered at 58 to 63 DEG C under 0.1 to 0.3 mPA 0.1 to 50 wt% of grape seed extract concentrated under reduced pressure using a vacuum rotation evaporator;
The water and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was refluxed and cooled at 100 ° C for 4 to 7 hours. The obtained extract was filtered, and the filtrate was filtered at 58 to 63 ° C under 0.1 to 0.3 mPa, 0.1 to 50 wt.% Of a saliva extract concentrated under reduced pressure using a vacuum rotation evaporator;
The pomegranate seed powder and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was refluxed and cooled at 100 ° C for 1 to 3 hours. The obtained extract was filtered, and the filtrate was filtered at 58 to 63 ° C under 0.1 to 0.3 mPa 0.1 to 50 wt% of pomegranate seed extract concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours;
The extract is filtered, and the filtrate is filtered at a temperature of 58 to 63 ° C and 0.1 to 0.3 mPA at a rate of 5 to 7 mPa, and the extract is filtered at a temperature of 65 to 75 ° C for 20 to 30 hours. By weight of a first mixture of 100 wt% composed of a mixture of 0.1 to 50 wt% of a pine needle extract concentrated under reduced pressure by a vacuum rotation evaporator for 0.01 to 10 wt% By weight, based on the total weight of the cosmetic composition.
The method according to claim 1,
The mixture of the outposts of Olefum and ethanol at a weight ratio of 1: 5 to 30 was shaken at 65 to 75 ° C at 100 to 120 rpm for 2 to 6 hours. The obtained extract was filtered, 0.1 to 50 wt% of an oat extract concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours;
Spirulina and distilled water were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken in an incubator at 20 to 30 ° C and 130 to 160 rpm for 50 to 80 hours. The resulting extract was centrifuged at 7,000 to 9,000 rpm for 10 to 30 minutes, After centrifugation for 30 minutes, the supernatant was filtered, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator at 0.1 to 0.3 mPA for 5 to 7 hours at 0.1 to 50 wt% of spirulina extract;
The fermented red ginseng and water are mixed at a weight ratio of 1: 5 to 30 and extracted at 80 to 93 ° C for 5 to 10 hours to obtain an extract. The obtained extract is filtered, 0.1 to 50 wt% of fermented red ginseng extract concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of mPA;
The upper leaves and ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 65 to 75 ° C at 100 to 120 rpm for 3 to 7 hours. The resulting extract was filtered, centrifuged at 4,000 to 6,000 rpm, 0.1 to 50 wt% of a leaf extract obtained by concentrating the supernatant under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA;
Safflower and ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was extracted at 60 to 100 ° C for 2 to 7 hours. The resulting extract was filtered, and the filtrate was vacuum-dried for 5 to 7 hours at a vacuum of 0.1 to 0.3 mPa and 0.1 to 50 wt% of a safflower extract concentrated under reduced pressure by a rotary evaporator to a composition of 100 wt% is further added so that the weight percentage of the composition is 0.01 to 10 wt% Wherein the cosmetic composition comprises a natural substance.
The method according to claim 1,
The bacterium powder and methanol were mixed at a ratio of 1: 5 ~ 30 by weight and extracted at 75 ~ 85 ℃ for 1 ~ 3 hours. The extracted extract was filtered and washed with 0.1 ~ 0.3 mPA for 5-7 hours 0.1-50 wt% of bokbule extract concentrated under reduced pressure with a rotary evaporator;
Chungma and distilled water were mixed at a weight ratio of 1: 5 ~ 30, and the mixture was extracted at 85 ~ 95 ℃ for 2 ~ 5h using a reflux extractor. The extract was centrifuged at 4 ~ The supernatant was separated and the supernatant was filtered to remove the remaining precipitates. The filtrate was concentrated to a concentration of 0.1 to 50 wt% in a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.
Doduk and methanol were mixed at a weight ratio of 1:20 to 22, and the mixture was added to a reflux extractor. While cooling water was supplied to a condenser at the upper end of the extractor, the extract was obtained by repeating 3 times at 58 to 62 ° C for 3 hours, Was filtered in a vacuum filtration apparatus and then concentrated under reduced pressure using a vacuum rotation evaporator at 0.1 to 0.3 mPA for 5 to 7 hours at a concentration of 0.1 to 50 wt%
The extract was filtered at a reduced pressure filtration apparatus, and then the filtrate was subjected to filtration under the conditions of 0.1 to 0.3 mPa, 0.1 ~ 50 wt.% Of a ginseng extract concentrated under reduced pressure with a vacuum rotation evaporator for 7 hours;
The mixture of peony and ethanol was mixed at a weight ratio of 1:10 to 12, and the mixture was immersed at room temperature for 22 to 26 hours. Separation and filtration of the supernatant were repeated three times, and 5 to 7 0.1 to 50 wt.% Of a crude extract obtained by concentrating the extract at a reduced pressure with a vacuum rotation evaporator for a period of time ranging from 0.01 to 10 wt.%, Based on 100 wt.% Of the third mixture, By weight, based on the total weight of the cosmetic composition.
The method according to claim 1,
The mixture of rice bran and ethanol was mixed at a weight ratio of 1:10 to 15, and the mixture was heated and extracted for 3 to 5 hours. The extracted extract was repeated three times and the filtrate was filtered at 58 to 63 ° C for 5 to 7 hours, 0.1-0.5 wt% of rice bran extract concentrated under reduced pressure with a vacuum rotation evaporator;
The crude extract and ethanol were mixed at a weight ratio of 1: 5 to 10, and then leached at room temperature for 24 hours. The primary extract was filtered, and the filtrate obtained by repeating the filtration twice by the same method was mixed, 0.1 to 50 wt.% Of a crude extract obtained by concentration under reduced pressure using a vacuum rotation evaporator;
The white blood and distilled water were mixed at a weight ratio of 1:15 to 20, heated and extracted at 100 ° C for 2 to 3 hours, centrifuged at 2,800 to 3,200 rpm for 10 to 20 minutes to remove debris, The supernatant is filtered in a vacuum filtration apparatus and then concentrated under reduced pressure using a vacuum rotation evaporator at 0.1 to 0.3 mPA for 5 to 7 hours.
The mushroom and ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was extracted at 120 to 180 ° C for 3 to 7 hours. The resulting extract was filtered, and the filtrate was concentrated under reduced pressure of 0.1 to 0.3 mPA for 5 to 7 hours vacuum rotation evaporator), 0.1 to 50 wt% of mushroom extract;
After mixing the yeast and ethanol at a weight ratio of 1:10 to 15, the extract was repeatedly extracted three times for 22 to 26 hours at room temperature. The extracted extract was filtered, and the filtrate was vacuum-condensed at 58 to 63 ° C for 5-7 hours and 0.1 to 50 wt% of a crude extract obtained by concentration under reduced pressure by an evaporator in an amount of 0.01 to 10 wt% based on 100 wt% of the mixture to be prepared Wherein the cosmetic composition contains a natural substance.
The method according to claim 1,
The mixture is mixed with methanol at a weight ratio of 1: 5 to 30, and the mixture is extracted with stirring at room temperature for 22 to 26 hours. The extract is filtered and concentrated under reduced pressure using a vacuum rotation evaporator. Wow,
The moss was washed with hot air at 55 to 65 ° C and then pulverized. The pulverized moss and 100% methanol were mixed in a ratio of 1: 5 to 30: 1 by weight. The mixture was cooled in a reflux condenser equipped with a cooling tube at 58 to 62 ° C for 5 to 7 The extract was filtered and concentrated under reduced pressure using a vacuum rotation evaporator. The extract was concentrated to a concentration of 0.01 to 50 wt%
Dried in a freeze drier and processed into a powder form. Powdered white radish and distilled water were mixed at a weight ratio of 1: 10 to 12: 1, and the mixture was refluxed at 100 ° C for 2 to 4 hours. The extract was filtered with a filter paper, and the filtered extract was concentrated with a vacuum rotation evaporator at a concentration of 0.1 to 0.3 mPA under reduced pressure for 5 to 7 hours at a concentration of 0.01 to 50 wt%
The mixture of grapefruit seed and ethanol was mixed at a weight ratio of 1: 5 to 10, and the mixture was refluxed for 2 to 3 hours at 60 to 62 ° C., centrifuged at 10,000 × g for 20 to 40 minutes, filtered, and a 100 wt% fifth mixture composed of 0.01 to 50 wt% of a grapefruit seed extract concentrated under reduced pressure for 5 to 7 hours under a vacuum rotation evaporator at 0.1 to 0.3 mPA, % To 0.01 to 3 wt%, so as to have a function of extending the shelf life of the cosmetic product.
Wherein the cosmetic composition is prepared by adding 0.1 to 50 wt% of the cosmetic composition according to any one of claims 1 to 6 to the total weight of the cosmetic composition.