KR102124592B1 - Hand-disinfectant composition for antibacterial and antivirus containing the extracts of natural material - Google Patents

Abstract

The present invention relates to a hand disinfectant composition having antibacterial and antiviral activity, containing a natural extract and, more specifically, to a hand disinfectant composition having antibacterial and antiviral activity, containing a natural extract, which comprises a bracken ethanol extract and a Lepidium meyenii extract as active ingredients, wherein the Lepidium meyenii extract is a Lepidium meyenii butanol fraction.

Description

Hand-disinfectant composition for antibacterial and antivirus containing the extracts of natural material}

The present invention relates to a hand sanitizer composition having antibacterial and antiviral activity containing a natural extract.

Hand sanitizers, which are currently sold as personal hygiene products, are made on the basis of alcohol, and are made by adding aloe vera to enhance moisture retention. Alcohol is usually produced at 60% or more, and in this case, it is necessary to prepare a hand sanitizer by adjusting the alcohol concentration to 60% or less because it is inconsistent with Korea's fire fighting method. Is required. In addition, since the irritating odor and odor caused by the large amount of alcohol contained in the hand sanitizer is a fundamental cause of deteriorating consumer preference, there is a need to select an excellent material having an effect of deodorizing activity to counteract it. As such a prior art, Patent No. 10-1130691 discloses a hand sanitizer composition for enhancing antimicrobial activity including a natural substance extract.

Hand sanitizers are currently used in parts of hospitals and marts in Korea, but they have not been widely used. On the other hand, it is widely used in everyday life in the United States and abroad. Hand sanitizers are widely used for the prevention of colds in addition to the purpose of preventing contamination of microorganisms through the hands.

On the other hand, ferns are distributed from tropical to temperate worldwide, with about 60 genera and 1500 species. Of these, 33 genera of 12 genera are used for medicinal purposes, and mainly the muscle and perianal regions are used. The distribution of ferns in Korea is widely distributed in the south and can be classified into 8 types of hemostatic drugs, 14 types of antidote drugs, and 24 types of blue fever. As a herbal medicine, the above-ground part is used, and as the flavonoid, astragalin, isoquerecetin, rutin, and tannin are included as a flavonoid (Medicinal Botany Rec. Soc. Korean, 2001) ). The fern (Pteridium aquilinum) that is cultivated in Korea belongs to the genus Pteridium (Dennsteadtiaceae). The entire plant has hairs, and root roots (root stems) crawl deeply in the ground and leaves are sparse. Ferns are harvested from April to May, and only young leaves are taken, boiled, dried, dried, and stored for edible use. In oriental medicine, the young leaves are called gyeok (뿌리), and the roots are called gyeokgeun (蕨根). When jaundice, sore throat, and high fever, the stem was cut at the root in the autumn and dried in the sun to be prescribed as a medicine.

Maca (Lepidium meyenii Walpers, maca) is a plant belonging to the family Brassisicaceae, and is an annual plant grown mainly in the highlands of Peru's Andes over 4,000m above sea level. It contains carbohydrates, proteins, lipids, vitamins, minerals, and fiber, and has a high nutritional value and has been found to exist in various physiological and functional substances such as alkaloids, steroids, tannins, and saponins.

Research on maca (maca) includes alcoholic beverages containing maca extract (Republic of Korea Patent Publication No. 10-2007-0003979), beverages that contain maca extract and have antioxidant and memory enhancing effects (Republic of Korea Patent Publication No. 10-2010- 0118816) and octacosanol and maca extract as an active ingredient, a cosmetic composition having an effect of increasing skin vitality (Republic of Korea Patent Publication No. 10-2010-0060780) has been disclosed. However, the above results do not consider the effect of the maca extract on antibacterial and antiviral diseases.

Thus, the present inventors completed the present invention by confirming that the composition of the present invention utilizing the fern extract and the maca butanol fraction has excellent efficacy as a hand sanitizer that promotes antibacterial and antiviral activity.

Republic of Korea Registered Patent No. 10-1130691 (2012.03.20)

Accordingly, an object of the present invention is to provide a hand disinfectant composition having antibacterial and antiviral activity containing fern hot water extract and maca butanol fraction as active ingredients.

In order to achieve the above object, the present invention includes fern hot water extract and maca extract as an active ingredient, and the maca extract is a hand sanitizer having antibacterial and antiviral activity containing a natural extract characterized in that it is a maca butanol fraction. Provided is a composition.

According to the present invention, the hand sanitizer composition of the present invention contains a natural extract, which has excellent antibacterial and antiviral activity and excellent human safety, and also contains a low content of alcohol, thereby reducing the irritating scent of alcohol and disinfecting hand sanitizer. By using it, it can prevent harmful bacteria residing in the skin and prevent infectious diseases including food poisoning and increase personal hygiene, thereby contributing to the promotion of national health.

In order to achieve the above object, the present invention includes fern ethanol extract and maca extract as an active ingredient, the maca extract is a hand sanitizer having antibacterial and antiviral activity containing a natural extract, characterized in that the maca butanol fraction Provided is a composition.

More preferably, the present invention provides a hand sanitizer composition comprising at least one of the group consisting of ethanol, carbomer, glycerin, propylene glycol, triethanolamine, aloe vera gel, hyaluronic acid and tocopherol.

The hand disinfectant composition of the present invention includes a viscosity modifier in order to form a formulation and impart a suitable viscosity for use, and among various viscosity modifiers, carbomer is most preferred in terms of mixability and usability with fern extract and maca extract. Do.

The hand disinfectant composition of the present invention also includes a carbomer neutralizing agent to assist in the formation of the viscosity of such carbomers, and such neutralizing agents are used alone or in combination with sodium hydroxide, potassium hydroxide, ammonium hydroxide, triethanolamine and the like. It is preferable, and triethanolamine is most preferable in terms of feeling of use and the like.

In the hand disinfectant composition of the present invention, as a moisturizing agent, aloe extracts such as glycerin, propylene glycol, hyaluronic acid or sodium hyaluronate, aloe vera gel, tocopherol, and the like may be used alone or in combination.

Preferably, the hand sanitizer composition of the present invention is 0.1 to 5% by weight of fern ethanol extract relative to the total weight of the composition, 0.1 to 5% by weight of maca butanol fraction, 20 to 50% by weight of ethanol, 0.1 to 5% by weight of carbomer, 0.1 to glycerin 5 wt%, propylene glycol 0.1 to 5 wt%, triethanolamine 0.1 to 5 wt%, aloe vera gel 0.1 to 5 wt%, hyaluronic acid 0.1 to 5 wt%, tocopherol 0.1 to 5 wt%, purified water 20 to 50 wt% %.

The hand sanitizer composition of the present invention may include additional components such as a surfactant, a flavoring agent, and a coloring agent within a range that does not impair the object of the present invention, but is not limited thereto.

Preferably, in the hand sanitizer composition of the present invention, the maca butanol fraction is obtained by sequentially extracting and separating maca methanol extract extracted with maca with hexane, ethyl acetate, butanol.

Hereinafter, the present invention will be described in more detail through examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

<Example 1>

1) Preparation of natural extracts

200 g of fern was extracted three times with 0.5 liter of ethanol at 45° C. for 24 hours, extracted and shaken for 24 hours, filtered and concentrated under reduced pressure at 60° C. with a rotary vacuum concentrator to obtain 30 ｇ of fern extract.

Thereafter, the maca powder was washed, dried, and dried to pulverize 100 g of maca powder three times at 45°C with 0.5 liter of methanol for 24 hours. Methanol extract was obtained 30 cc. After adding 0.5 liter of water to the methanol extract and suspending it, the mixture was extracted by adding 0.5 liter of hexane twice, followed by separating the hexane layer and concentrating the hexane layer under reduced pressure to obtain a hexane fraction. Subsequently, the remaining aqueous layer was successively fractionated with 0.5 liter of ethyl acetate and 0.5 liter of butanol in the same manner as described above, and then concentrated under reduced pressure to obtain an ethyl acetate fraction and a butanol fraction, respectively.

Thereafter, fern ethanol extract and maca butanol fraction were used in the following hand sanitizer composition.

2) Preparation of hand sanitizer composition

The fern ethanol extract and maca butanol fraction were used in preparing the hand sanitizer composition according to Table 1 below. To prepare the hand sanitizer composition, carbomer was dissolved in purified water, dissolved by adding fern ethanol extract, maca butanol fraction, ethanol, glycerin and propylene glycol, and then triethanolamine was added to form a viscosity. Aloe vera gel, hyaluronic acid and tocopherol were added thereto to prepare the mixture by stirring.

<Example 2>

In the same manner as in Example 1, the composition of Example 2 was prepared under the conditions shown in Table 1 below.

<Comparative Examples 1 to 7>

In the same manner as in Example 1, but for each of the fern extract and maca extract, Comparative Example 1 is a fern ethanol extract (excluding the maca butanol fraction), Comparative Example 2 is a maca butanol fraction (excluding the fern ethanol extract), Comparative Example 3 Silver fern hot water extract, Comparative Example 4 is fern hot water extract and maca butanol fraction, Comparative Example 5 is fern ethanol extract and maca methanol extract, Comparative Example 6 is fern ethanol extract and maca hexane fraction, Comparative Example 7 is fern ethanol extract and maca Using the ethyl acetate fraction, the compositions of Comparative Examples 1 to 7 were prepared under the conditions shown in Table 1 below.

Ingredient (% by weight) Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 95% ethanol 42.0 42.0 42.0 42.0 42.0 42.0 42.0 42.0 42.0 Carbomer 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 Propylene glycol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Triethanolamine 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Aloe veragel 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Hyaluronic acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Tocopherol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Fern extract One 2 3 - 3 One One One One Maca extract 2 One - 3 - 2 2 2 2 Purified water 51.7 51.7 51.7 51.7 51.7 51.7 51.7 51.7 51.7 Sum 100 100 100 100 100 100 100 100 100

<Experimental Example 1> Antibacterial activity confirmation

After processing the samples of Examples and Comparative Examples on a paper disc, the disks were arranged at regular intervals under a sterile operation on the medium on which the strain was spread, and cultured for 2 days to measure antibacterial activity. Evaluation of the antibacterial activity of the sample was evaluated by measuring the diameter (mm) of the growth inhibition region of the bacteria around the disk. Staphylococcus aureus and Salmonella were used as strains to be tested, and each strain can be used by pre-sale from a domestic microorganism depository institution (Biotechnology Research Institute Genetic Bank, Korea Microbial Conservation Center, Agricultural Gene Resource Center).

The strains were inoculated in a medium (Malt Extract Agar 6%, Ox-bile 2%, Tween-40 1%, Glycerol mono-oleate 025%) and incubated at 37°C for 24 hours.

The cultured strains were diluted to 1/100 ratio and plated on a solid medium of the plate, and the growth inhibition ring (growth inhibition zone) was measured after 2 days of arranging the disc. The growth inhibition ring measurement results are shown in Table 2 below.

division
(Unit: mm) Staphylococcus aureus Salmonella Typhimurium Example 1 23.3 22.4 Example 2 21.6 20.4 Comparative Example 1 12.5 11.6 Comparative Example 2 11.6 10.6 Comparative Example 3 4.3 3.2 Comparative Example 4 6.5 4.5 Comparative Example 5 13.4 11.2 Comparative Example 6 13.6 10.4 Comparative Example 7 14.3 12.3

As shown in Table 2, the antibacterial activity of Comparative Example 1 (fern ethanol extract) and Comparative Example 2 (maca butanol fraction) is low, while Comparative Example 3 (fern brackish water extract) has very low antibacterial activity, whereas fern ethanol extract The hand sanitizer compositions of Example 1 and Example 2 in which the and maca butanol fractions were mixed exhibited significantly increased antibacterial activity.

<Experimental Example 2> Confirmation of antiviral activity

After influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with MDCK (Mardin Darby Canine Kidney) cell line, each of the compositions of Example 1, Example 2, and Comparative Examples 1 to 2 was 2 μg-10. Antiviral activity was analyzed by treatment with a concentration range of mg/ml.

MDCK cell lines used in the experiment were cultured regularly in Eagle's minimum essential medium (MEM) containing 10% fetal bovine serum (FBS), Hyclone Thermo Scientific and 1% penicillin-streptomycin solution (Gibco). I prepared it.

1×10 ⁴ MDCK cells per ml were dispensed into 96-well plates and cultured at 37° C. for 16 hours using a 5% CO ₂ incubator. MDCK cells of a single layer were washed twice with PBS, and then influenza virus type A (H1N1) and seasonal influenza virus type A (H3N2) were infected with 100 TCID ₅₀ at 37°C for 2 hours, followed by washing of uninfected viruses. Came out.

Infected cells were serially diluted in a virus culture solution (0.3% Bovine serum albumin, 1% Penicillin-Streptomycin solution) containing the compositions of the above Examples and Comparative Examples and 1 μg/ml L-1-tosylamido-2-phenylethyl The cells were incubated at 37°C for 48 hours until a viral cytopathic effect (CPE) was observed in the MEM culture medium containing trypsin-TPCK treated with chloromethyl ketone. The antiviral CPE-lowering ability of the compositions was expressed as a 50% value (EC ₅₀ ) for viral inhibition, and a 50% value (CC ₅₀ ) of cytotoxicity concentration was determined based on morphological modification of cells. The ability of the anti-influenza virus of the compositions was expressed by the selectivity index (SI) divided by the CC ₅₀ value divided by the EC ₅₀ value. SI (selectivity index, selectivity index) is CC ₅₀ /EC ₅₀ , the larger the SI value, the greater the virus growth inhibitory effect. Table 3 shows the results of comparing the anti-influenza virus activity against H1N1 and H3N2 of the compositions in MDCK cells.

division CC ₅₀ (μg/ml) EC ₅₀ (μg/ml) SI H1N1 H3N2 H1N1 H3N2 H1N1 H3N2 Example 1 7247 7238 74.6 108.5 97.1 66.7 Example 2 7180 7154 76.1 112.7 94.3 63.5 Comparative Example 1 7345 7388 313.4 351.8 23.4 21.0 Comparative Example 2 7668 7660 284.0 312.7 27.0 24.5 Comparative Example 3 - - - - - - Comparative Example 4 7294 7296 210.8 215.2 34.6 33.9 Comparative Example 5 6945 6989 213.0 222.6 32.6 31.4 Comparative Example 6 7548 7580 246.7 256.9 30.6 29.5 Comparative Example 7 7439 7450 202.7 228.5 36.7 32.6

As can be seen in Table 3, in relation to the anti-influenza virus activity against H1N1, the SI value of Comparative Example 1 (fern Fern ethanol extract) is 23.4, Comparative Example 2 (Maca butanol fraction) is 27.0, while Fern ethanol extract It can be seen that Example 1 in which the and maca butanol fractions were mixed showed a remarkably elevated effect to 97.1.

In addition, Comparative Example 3 (Hornia hot water extract) showed almost no anti-influenza virus activity, and Comparative Examples 5 to 7 were mixed with Maca methanol extract, Maca hexane fraction, and Maca ethyl acetate fraction, respectively, in Fern ethanol extract. Compared to Example 1 in which the ethanol extract and the maca butanol fraction were mixed, the SI value of Example 1 was significantly increased.

Meanwhile, the above detailed description should not be construed as being limited in all respects, but should be considered as illustrative. The scope of the invention should be determined by rational interpretation of the appended claims, and all changes within the equivalent scope of the invention are included in the scope of the invention.

Claims (3)

Contains fern ethanol extract and maca extract as active ingredients,
The maca extract is a hand sanitizer composition having an antibacterial and antiviral activity containing a natural extract characterized in that it is a maca butanol fraction obtained by sequentially extracting and separating maca methanol extract extracted with maca with hexane, ethyl acetate, and butanol. .
According to claim 1,
The composition is a hand sanitizer composition having an antibacterial and antiviral activity containing a natural extract containing at least one of the group consisting of ethanol, carbomer, glycerin, propylene glycol, triethanolamine, aloe vera gel, hyaluronic acid and tocopherol .
According to claim 1,
The fern ethanol extract is a hand sanitizer composition having an antibacterial and antiviral activity containing a natural extract, characterized in that the fern is extracted three times with ethanol at 45°C for 24 hours and concentrated under reduced pressure with a rotary vacuum concentrator.