KR101921211B1 - Cosmetic composition for skin regeneration comprising Ainsliaea acerifolia extract or its fraction as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition for regenerating skin containing an extract of a single-puff extract or a fraction thereof as an active ingredient. More particularly, the present invention relates to a cosmetic composition for skin regeneration, which is safe and has little toxicity and side effects, And since the skin regeneration effect is excellent, the composition of the present invention containing it as an active ingredient may be usefully used as a material for functional cosmetics for skin regeneration which does not cause irritation to the skin.

Description

[0001] The present invention relates to a cosmetic composition for skin regeneration comprising an extract of A. pneumoniae or a fraction thereof as an active ingredient,

The present invention relates to a cosmetic composition for skin regeneration, which comprises an extract of Artemisia oryzae or a fraction thereof as an active ingredient.

Skin is the primary protective membrane of the human body. It protects the internal organs from external stimuli such as temperature and humidity changes, ultraviolet rays, and pollutants, and plays an important role in keeping the body homeostatic such as body temperature control. The keratin, which is located at the outermost part of the skin, is a tissue formed by the change of skin cells. The stratum corneum is composed of dead cells. In the dermal layer, the dermally formed cell layer in the connective tissue is lifted to the epidermis, and the cell becomes viable and changes into a firm and regular cell layer. The nucleus and cytoplasm escape and form hard stratum corneum like bricks. This stratum corneum acts as a defense line to protect the moisture of our body from the outermost part of the skin tissue and to protect the various substances coming in from outside while firmly supporting the skin.

Skin cell formation and keratinization are important cycles to maintain skin health. If the skin cell regeneration ability is good, the skin cells are rapidly formed in the dermis layer and quickly become keratinized, damaged by external stimuli, and aged keratin is replaced with newly formed keratin to help maintain healthy skin. As with other organs of the human body, the skin becomes less senile and aging progresses with age. As a result, the elasticity of the skin is lost, the old horny skin is not replaced, the skin becomes horny, The structure and fibers are reduced, wrinkles are generated, and the skin is shrunken.

Accordingly, efforts have been made to develop physiologically active substances having skin regeneration-promoting effect in order to maintain healthy skin by maintaining skin regeneration ability and maintaining healthy and elastic skin. Tretinoin (trans-retinoic acid) and retinol have begun to be used in cosmetic raw materials and since then, female hormone-like substances and antioxidants extracted from various plants have been introduced into cosmetics as a raw material for such efficacy. However, most of these cosmetic raw materials have various problems such as insufficient effects or side effects such as skin irritation. Accordingly, researches on raw materials having skin regeneration improving effect without inducing skin side effects are actively proceeding.

On the other hand, Ainsliaea acerifolia is a perennial herbaceous plant of Asteraceae which grows in mountains of each country. The growing environment grows in moist, partly cloudy. It is largely distributed in Korea, China and Japan. There are no studies on the physiological functioning of sweetweeds in addition to the use of lice blossoms in folk remedies and edible foods.

Meanwhile, Korean Patent Laid-Open Publication No. 2017-0000888 discloses a health functional composition using a single-puff extract and a functional beverage containing the extract, but it has not yet been found that the cosmetic composition for skin regeneration containing the extract of the present invention or the fraction thereof as an active ingredient .

The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a skin regeneration ability reduced by UV irradiation when treated with fibroblast (HS68) cells, respectively, , The biscuit enzyme treatment, and the ultraviolet pressure homogenization treatment were superior in skin regeneration effect to that of the above-mentioned single-bladed ethanol extract, the present invention was completed.

In order to achieve the foregoing object, the present invention danpungchwi (Ainsliaea acerifolia ) extract or a fraction thereof as an active ingredient.

The extract of the present invention can be used for skin for a long period of time because it is safe, has little toxicity and side effects, and has excellent skin regeneration effect. Therefore, the composition of the present invention containing the extract as an active ingredient, It can be usefully used as a functional cosmetic material for skin regeneration that does not cause skin irritation.

FIG. 1 is a graph showing the cell viability of HS68 cells treated with the ethanol extract of A. japonica and fractions thereof according to an embodiment of the present invention. CON is a control group with no treatment.
FIG. 2 is a result of examining the skin regeneration efficacy of the ethanol extract of A. bovis and its fractions according to an embodiment of the present invention. CON is a control group with no treatment.
FIG. 3 is a graph showing the skin regeneration effects of each of the single-puff extracts prepared by treating the single-bladed ethanol extract, the enzyme type treatment, the ultra-high pressure homogenization treatment, and the enzyme treatment with the ultra-high pressure homogenous combination treatment according to an embodiment of the present invention. CON is a control group with no treatment.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for skin regeneration comprising an extract of Ainsliaea acerifolia or a fraction thereof as an active ingredient.

In the cosmetic composition for skin regeneration of the present invention, the above-mentioned single-puff extract can be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably ethanol, It does not.

In the cosmetic composition for skin regeneration of the present invention, the above-

1) adding Pectinex to a mixture of water and water, and then hydrolyzing the mixture; And

2) extracting the hydrolyzate of step 1) with ethanol,

Preferably,

1) Pectinex is added to a mixture of 10-14 times (v / w) of water adjusted to pH 3.5-5.5 and the hydrolysis is carried out at 25-45 ° C for 1-3 hours. ; And

2) adding 2 to 3 times (v / v) 100% ethanol to the hydrolyzate of step 1), and extracting the mixture at 20 to 30 ° C for 2 hours.

More preferably,

1) adding pectinex to a mixture obtained by adding 13 times (v / w) of water adjusted to pH 4.5 to a single-stranded powder, and then hydrolyzing the mixture at 37 ° C for 2 hours; And

2) adding 100% ethanol to the hydrolyzate of step 1) in an amount of 2 to 3 times (v / v) and extracting the mixture at 20 to 30 ° C for 2 hours, but it is not limited thereto .

In addition, the above-

1) adding a viscozyme to the mixture to which water is added to the monodisperse powder and then hydrolyzing the mixture;

2) extracting the hydrolyzate of step 1) with ethanol; And

3) subjecting the ethanol extract of step 2) to ultra-high pressure homogenization,

Preferably,

1) adding Viscozyme to a mixture of 10-14 times (v / w) of water adjusted to pH 3.5-5.5 in the dried pale yellow powder and hydrolyzing the mixture at 30-60 ° C for 8-12 hours ;

2) adding 2 to 3 times (v / v) 100% ethanol to the hydrolyzate of step 1) and extracting at 20 to 30 ° C for 1 to 3 hours; And

3) homogenizing the ethanol extract of step 2) at a pressure of 110 to 130 MPa under ultrahigh pressure,

More preferably,

1) adding a viscozyme to a mixture obtained by adding 13-fold (v / w) of water adjusted to pH 4.5 to the odoriferous dry powder, and then hydrolyzing the mixture at 50 ° C for 10 hours;

2) adding 2 to 3 times (v / v) 100% ethanol to the hydrolyzate of step 1) and extracting at 20 to 30 ° C for 2 hours; And

3) homogenizing the ethanol extract of step 2) at 120 MPa under high pressure, but the present invention is not limited thereto.

In addition, the fraction may be, but is not limited to, the ethyl acetate fraction of the ethanol extract of A. pneumoniae.

In addition, the cosmetic composition may be in the form of a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, an eye cream, a moisturizer cream, But is not limited to, one selected from the group consisting of a cleansing foam, a cleansing water, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a soap and a powder.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is an interfacial active agent-containing cleansing, it is preferable to use, as carrier components, aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, perfumes, surfactants, May further comprise one or more adjuvants selected from among emulsifying agents, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, But is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Materials and methods

One. A single blow  Extract and its Fraction  Produce

In order to examine the skin regeneration efficacy of the ethanol extract of the present invention and its fractions, the treatment of each kind of enzyme, and the skin rehabilitation efficacy according to the presence or absence of the ultrahigh pressure homogenization, the ethanol extract and its fractions were treated according to the enzyme type, the presence of ultra high pressure homogenization, Deep-blotting extracts according to the treatments were prepared as follows.

(1) Ethanol extraction and its fraction

30 g of 70% (v / v) ethanol was added to 3 g of fresh-air dried powder, and the ethanol extract was extracted at room temperature for 12 hours, filtered through a filter paper, concentrated and freeze-dried.

In order to produce the fraction of the above-mentioned single-blasted ethanol extract, hexane was added to the above-mentioned single-blasted ethanol extract, and the fraction was divided into a nucleic acid layer and an aqueous layer with a separating funnel. The nucleic acid fraction was concentrated under reduced pressure to obtain a nucleic acid fraction. Acetate, n-butanol and water were fractionated to obtain ethyl acetate fraction, n-butanol fraction and water fraction.

(2) Enzyme treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of Viscozyme and Pectinex were added to 2.5 g of freshly pungent powder and 50 units / ml and 500 unit / ml, respectively, and the mixture was heated at 50 ° C and 37 ° C at 180 rpm Lt; / RTI > After the enzymes were treated for optimal time (10 hours and 2 hours), 70 ml of 100% (v / v) ethanol was added, and the mixture was extracted at 180 rpm for 2 hours at room temperature. After centrifugation at 12,000 rpm for 15 minutes, Respectively.

(3) Ultra high pressure homogenization treatment

30 ml of distilled water adjusted to pH 4.5 and 70 ml of 100% (v / v) ethanol were added to 2.5 g of freshly pungent powder and stirred. The mixture was centrifuged at 12,000 rpm for 15 minutes and filtered to prepare a filtrate. Lt; RTI ID = 0.0 > of < / RTI > the orifice tube.

(4) Enzyme treatment and high pressure homogenization parallel treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of Viscozyme and Pectinex were added to 2.5 g of freshly pungent powder and 50 units / ml and 500 unit / ml, respectively, and the mixture was heated at 50 ° C and 37 ° C at 180 rpm Lt; / RTI > The enzyme was treated at the optimal time (10 hours and 2 hours), and then 70 ml of 100% (v / v) ethanol was added. The mixture was extracted at 180 rpm for 2 hours at room temperature (25 ° C) and centrifuged at 12,000 rpm for 15 minutes After filtration, the filtrate was passed through an orifice tube twice at a pressure of 120 MPa.

2. Cell culture and cytotoxicity measurement

Cells used in the present invention were obtained from ATCC (American Type Culture Collection, CRL-1635) as human normal fibroblasts (HS68). Cell culture was done using 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin (100 U / mL) to a DMEM was added (Dulbeco's modified eagle's medium, Gibco Co, Gaithersburg, MD, USA) medium, CO ₂ And cultured in an incubator (37 ° C, 5% CO ₂ , Sanyo, Japan).

In order to investigate the concentration range of cytotoxicity of the extract from the leaves and their fractions, MTT (3- ( 4,5-dimeth-ylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) analysis. HS68 cells were inoculated in a 96-well plate at a concentration of 1 × 10 ⁵ cells / mL and cultured for 24 hours. Then, various concentrations of the dried leaf extract and its fractions were treated and further cultured for 24 hours. 10 μl of 5 mg / ml MTT solution was added to each well and the reaction was allowed to proceed for 3 hours. After removing the supernatant, 100 μl of DMSO (dimethyl sulfoxide) solution was added to completely dissolve purple formazan crystals Respectively. Absorbance was measured at 570 nm using a spectrophotometer. Relative cell viability was calculated for the single-puff extract of the present invention and its fraction-treated group based on the survival rate (100%) of the untreated group treated with no cytotoxicity.

3. Skin cell Playability  Measure

HS68 cells were plated at 1 x 10 < ⁵ > cells / mL , And then cultured in a CO ₂ incubator for 24 hours. Then, the medium was removed, and 500 μl of PBS was added. The plate was irradiated with UVC (254 nm) at a distance of 650 mm for 1 minute and 20 seconds. After irradiation with UVC, the serum-free DMEM medium, the monocotyledonous extract and the fractions thereof were treated for each concentration and cultured for 48 hours. After incubation, 50 μl of MTT reagent was added and left for 3 hours. After removing the supernatant, 100 μl of DMSO (dimethyl sulfoxide) was added to the formed formazan, and the absorbance was measured at 570 nm for 30 minutes.

Example  One. A single blow  Extract and its Fraction Cytotoxicity by treatment  And skin regeneration effect

In this Example 1, the extract of the single-puff extract and its fractions were treated with HS68 cells at different concentrations to confirm cytotoxicity. As a result, it was found that, when treated with the extracts and fractions of cutwax leaves other than 100 μg / ml of hexane fraction and 100 μg / ml of ethyl acetate fraction as shown in FIG. 1, cell survival rate was not significantly decreased, I could confirm. As a result of examining the skin regeneration effect of the extract from the leaves and the fractions thereof, it was found that when ethanol extract of 100 .mu.g / ml was treated with ethanol extract, the skin regeneration ability decreased due to UV irradiation and the ethyl acetate fraction / Ml), it was confirmed that the reduced skin regeneration ability was increased by the UV treatment (FIG. 2).

Example  2. Enzyme treatment and ultrahigh pressure Homogenization  Processed A single blow  Skin regeneration effect of extract

In Example 2, the skin regeneration effects of the single-bladed-ventilation ethanol extracts prepared according to the enzymatic type treatment, the presence / absence of ultra-high pressure homogenization, and the combined treatment with the enzyme treatment + ultra-high pressure homogenization were compared . As a result, as shown in Table 1, when treated with the ethanol extract of monocotyledonous bacterium (77.10 ± 2.88) and the monodods extract (Pectinex; 78.15 ± 4.70) prepared by treating pectinex enzyme, (Vis + ultrahigh pressure homogenisation: 80.57 +/- 2.37), which was prepared by using Viscozyme enzyme treatment and ultrahigh pressure homogenization in parallel, was remarkably superior in skin regeneration ability (FIG. 3).

The result of measuring the skin regeneration ability by treatment with the single blight extract (100 μg / ml) Processing method Skin regeneration (%) NO UV 100 UV 55.24 + - 1.02 Ethanol extract 77.10 ± 2.88 Viscozyme 72.19 ± 3.10 Pectinex 78.15 + - 4.70 Ultrahigh pressure homogeneity 74.75 ± 6.13 Viscozyme + ultrahigh pressure homogeneity 80.57 + - 2.37 Pectinex + ultrahigh pressure homogeneity 53.61 ± 2.92

Claims (7)

A cosmetic composition for regenerating skin comprising an extract of Ainsliaea acerifolia or a fraction thereof as an active ingredient, wherein the extract is an extract prepared by adding Pectinex to a pale breeze powder and hydrolyzing it after ethanol extraction; Or viscose is added to monodisperse powder to hydrolyze, followed by ethanol extraction and ultra-high pressure homogenization. The fraction is an ethylacetate fraction of ethanol extract A cosmetic composition for skin regeneration. delete delete delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, The cosmetic composition for skin regeneration according to any one of claims 1 to 3, wherein the composition is one selected from the group consisting of an essence, a pack, a cleansing foam, a cleansing water, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a soap and a powder. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises at least one of a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a perfume, Or one or more adjuvants selected from nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, Wherein the cosmetic composition is a cosmetic composition for skin regeneration.

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