KR101901133B1 - Cosmetic composition for skin anti-wrinkle comprising Heracleum moellendorffii extract as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin wrinkle reduction comprising a Heracleum moellendorffii extract as an effective component. More specifically, the Heracleum moellendorffii extract of the present invention is a natural extract, thereby being safe and not causing side effects to the skin; remarkably increases the generation amount of collagen to provide a skin wrinkle reduction effect. Therefore, the composition of the present invention which contains the Heracleum moellendorffii extract as an effective component can be usefully used as a material for a functional cosmetic product for reducing skin wrinkles.

Description

[0001] The present invention relates to a cosmetic composition for improving skin wrinkles containing an aquatic extract as an active ingredient,

The present invention relates to a cosmetic composition for improving skin wrinkles containing an aquatic extract as an active ingredient.

Skin is the frontal defense body of the human body that protects our body from external stimuli, and its interest is increasing from an aesthetic point of view. Accordingly, various functional cosmetics have been actively researched. Among them, various functional cosmetics for wrinkle reduction and whitening function are being marketed.

Several causes of wrinkles have been reported. First, when the skin is exposed to excessive ultraviolet rays, collagen decomposition, which is a component of the skin, is accelerated, and the skin may lose elasticity and wrinkles may be formed. In addition, if the skin becomes excessively dry due to excessive temperature change, humidity reduction, wind or the like, skin function as a barrier against the outside may be deteriorated and wrinkles may occur. When the skin is exposed to reactive oxygen species or free radicals, lipid peroxidation is produced by the oxidative action, and skin texture proteins such as collagen may be deformed, resulting in wrinkles. Various cosmetic compositions have been studied to suppress wrinkles caused by external factors and internal factors. In particular, functional cosmetics containing chemical components cause skin irritation, which is not suitable for sensitive skin, and attention is focused on functional cosmetics derived from natural plants that are less irritating to skin and environmentally friendly.

On the other hand, eosuri (Heracleum moellendorffii Hance) is a perennial herbaceous herbaceous perennial herbaceous plant, distributed in Korea, China and Japan. It grows in mountains and fields, stems straight, 70-150cm high, hollow cylindrical, with vertical streaks, coarse hairs, and thick branches. It is a pinnately compound leaf composed of 3 to 5 leaves with alternate leaves. It has hairs. As it goes above the stem, the petiole becomes shorter and the lower part becomes wider and covers the stem. The small leaf attached to the end is heart-shaped and is divided into 3 pieces. The small leaf attached to the side is broad egg-shaped or triangular, 7-20cm long, split into 2-3 pieces, and there are sawteeth deeply pitted. The flowers bloom in July to August in white, and they run on the tips of branches and trunks in a double-inflorescence. The inflorescence is divided into 20 ~ 30 flower stalks again divided into small flower sacks, each with 25 ~ 30 flowers, and the flowers on the edge are larger than those in the middle. There are 6 petals and they are different in size. Outer petals are larger than inner petals. The fruit is a division (fruit divided from the division) and has a flat egg with a length of 7mm upside down and a unique pattern on the upper part. It is mainly used as herbal medicines in oriental medicine, and it is also used as food of herbs.

On the other hand, Korean Patent No. 1313255 discloses a flame-retardant composition comprising an aquatic natural essential oil extracted from a leaf of an aquarium, but the cosmetic composition for improving skin wrinkles containing the aquatic extract of the present invention as an effective ingredient There is no bar.

The present invention provides a cosmetic composition for improving skin wrinkles containing an aquatic extract as an active ingredient. The present invention provides a cosmetic composition for improving skin wrinkles, wherein the active ingredient, aquatic extract, significantly increases collagen production, And confirming that the improving effect is excellent, the present invention has been completed.

In order to achieve the foregoing object, the present invention eosuri (Heracleum The present invention provides a cosmetic composition for improving skin wrinkles, which comprises, as an active ingredient, an extract of Mellendorffii .

Since the aquatic extract of the present invention is a natural-derived extract, it has a safety feature that does not cause side effects to the skin and remarkably increases the amount of collagen production, thereby improving skin wrinkles. Therefore, the composition of the present invention, It can be usefully used as a functional cosmetic material for improving skin wrinkles which does not cause irritation.

FIG. 1 is a graph comparing the efficacy of aquaculture extracts prepared according to an embodiment of the present invention on the skin wrinkle-reducing effect of each of the aquatic extracts prepared by treatment with different types of enzymes, ultra-high pressure homogenization treatment, and enzyme treatment and ultrahigh pressure homogeneous concurrent treatment. CON is a control group with no treatment.

According to an aspect of the invention, the invention is eosuri (Osmunda japonica ) extract as an active ingredient.

In the cosmetic composition for improving skin wrinkles of the present invention, the aquatic extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably ethanol, It is not limited.

In addition, the above-

1) adding an enzyme to a mixture of water of an aquatic powder and hydrolyzing the mixture; And

2) treating the hydrolyzate of step 2) with ethanol,

Preferably,

1) adding Viscozyme to a mixture of 10- to 14-fold (v / w) of water adjusted to pH 3.5-5.5 in australia powder and hydrolyzing the mixture at 30 to 60 ° C for 8 to 12 hours ; And

2) Extracting the hydrolyzate of step 1) by adding 2 to 3 times (v / v) 100% ethanol at 20 to 30 ° C for 1 to 3 hours,

More preferably,

1) adding a viscozyme to a mixture of 13 times (v / w) of water adjusted to pH 4.5 in australia powder, and hydrolyzing the mixture at 50 ° C for 10 hours; And

2) Extracting the hydrolyzate of step 1) by adding 2 to 3 times (v / v) of 100% ethanol and extracting at 20 to 30 ° C for 2 hours, but the present invention is not limited thereto.

In addition, the skin wrinkles may be induced by ultraviolet irradiation, but are not limited thereto.

In addition, the cosmetic composition may be in the form of a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, an eye cream, a moisturizer cream, But is not limited to, one selected from the group consisting of a cleansing foam, a cleansing water, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a soap and a powder.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is an interfacial active agent-containing cleansing, it is preferable to use, as carrier components, aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, perfumes, surfactants, May further comprise one or more adjuvants selected from among emulsifying agents, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, But is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Materials and methods

One. Aquarius  Extract preparation

In order to confirm the wrinkle-reducing effect of the aquatic ethanol extract of the present invention and the treatment of each type of enzyme and the effect of high-pressure homogenization on the wrinkle, Respectively.

(1) Ethanol extraction

After adding 30 ml of 70% (v / v) ethanol to 3 g of aquatic flour, the ethanol extract extracted at room temperature for 12 hours was filtered through a filter paper, concentrated and freeze-dried.

(2) Enzyme treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of Viscozyme and Pectinex were added to 2.5 g of aquatic flour and 50 units / ml and 500 unit / ml, respectively. Lt; / RTI > After the enzymes were treated for optimal time (10 hours and 2 hours), 70 ml of 100% (v / v) ethanol was added, and the mixture was extracted at 180 rpm for 2 hours at room temperature. After centrifugation at 12,000 rpm for 15 minutes, Respectively.

(3) Ultra high pressure homogenization treatment

30 ml of distilled water adjusted to pH 4.5 and 70 ml of 100% (v / v) ethanol were added to 2.5 g of aquatic flour and stirred. The mixture was centrifuged at 12,000 rpm for 15 minutes and filtered to prepare a filtrate. Lt; RTI ID = 0.0 > of < / RTI > the orifice tube.

(4) Enzyme treatment and high pressure homogenization parallel treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of Viscozyme and Pectinex were added to 2.5 g of aquatic flour and 50 units / ml and 500 unit / ml, respectively. Lt; / RTI > The enzyme was treated at the optimal time (10 hours and 2 hours), and then 70 ml of 100% (v / v) ethanol was added. The mixture was extracted at 180 rpm for 2 hours at room temperature (25 ° C) and centrifuged at 12,000 rpm for 15 minutes After filtration, the filtrate was passed through an orifice tube twice at a pressure of 120 MPa.

2. Cell culture

Cells used in the present invention were obtained from ATCC (American Type Culture Collection, CRL-1635) as human normal fibroblasts (HS68). Cell culture was done using 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin (100 U / mL) to a DMEM was added (Dulbeco's modified eagle's medium, Gibco Co, Gaithersburg, MD, USA) medium, CO ₂ And cultured in an incubator (37 ° C, 5% CO ₂ , Sanyo, Japan).

3. PIP ( Procollagen  type I C-peptide measurement

PIP content was measured using PIP (procollagen type I C-peptide) EIA kit (Takara, Korea). The cells 1 × 10 ⁵ cells / mL , And the cells were cultured in a CO ₂ incubator for 24 hours and then measured using a culture solution. Add 100 μl of antibody-POD conjugate solution and 20 μl of aquatic extract to the 96-well plate in the kit, mix well and incubate at 37 ° C for 3 hours. Remove the contents and wash 4 times with Wash Buffer gave. Next, 100 μl of TMBZ (substrate solution) was added, reacted at room temperature for 30 minutes, and 100 μl of stop solution was added to stop the reaction, and the absorbance was measured at 450 nm.

Example  One. Aquarius  Collagen Biosynthesis amount  Increase effect

In Example 1, ultraviolet rays were irradiated to skin fibroblasts, and then the PIP (Procollagen type I C) of the aquatic extracts prepared according to the enzyme water treatment, the treatment with the enzyme type, the presence of ultra high pressure homogenization and the enzyme treatment + -petide) content of the extract. As a result, when the ethanol extract (EtOH extract; 68.74 +/- 0.64) was treated as described in Table 1 and FIG. 1, the decreased PIP content due to the UV treatment was increased, and the paprika produced by treatment with the viscose enzyme When the extract (Viscozyme; 71.58 ± 1.16) was treated, the PIP content was found to be higher than that of the ethanol extract. In the case of aquaculture (Vis + ultrahigh pressure homogenate; 55.97 ± 3.96, Pec + ultrahigh pressure homogeneity; 63.40 ± 2.37) prepared by using ultra high pressure homogenization (44.29 ± 1.89) or high pressure homogenization after enzyme treatment, It was confirmed that the PIP content was further reduced.

Measurement of PIP content change by treatment with aquatic extract Processing method PIP content (%) NO UV 100 UV 55.24 + - 1.02 EtOH extract 68.74 + 0.64 Viscozyme 71.58 ± 1.16 Pectinex 68.90 + - 2.67 Ultrahigh pressure homogeneity 44.29 ± 1.89 Viscozyme + ultrahigh pressure homogeneity 55.97 ± 3.96 Pectinex + ultrahigh pressure homogeneity 63.40 ± 2.37

Claims (7)

A cosmetic composition for improving skin wrinkles containing an extract of Herculesum moellendorffii ethanol as an active ingredient. delete The method according to claim 1, wherein the aquatic ethanol extract
1) adding an enzyme to a mixture of water of an aquatic powder and hydrolyzing the mixture; And
2) extracting the hydrolyzate of step 1) with ethanol, and extracting the extract with ethanol. The cosmetic composition for improving skin wrinkles according to claim 3, wherein the enzyme of step 1) is Viscozyme. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the skin wrinkles are induced by ultraviolet irradiation. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, The cosmetic composition for improving skin wrinkles according to any one of claims 1 to 3, wherein the composition is one selected from the group consisting of an essence, a pack, a cleansing foam, a cleansing water, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a soap and a powder. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises at least one of a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a perfume, Or one or more adjuvants selected from nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, Wherein the cosmetic composition is a cosmetic composition for improving skin wrinkles.

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