KR101795272B1 - Cosmetic Composition comprising Leibnitzia anandria extract having the effect of regulating the loss of subcutaneous fat - Google Patents
Cosmetic Composition comprising Leibnitzia anandria extract having the effect of regulating the loss of subcutaneous fat Download PDFInfo
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- KR101795272B1 KR101795272B1 KR1020160073237A KR20160073237A KR101795272B1 KR 101795272 B1 KR101795272 B1 KR 101795272B1 KR 1020160073237 A KR1020160073237 A KR 1020160073237A KR 20160073237 A KR20160073237 A KR 20160073237A KR 101795272 B1 KR101795272 B1 KR 101795272B1
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- extract
- cosmetic composition
- present
- skin
- subcutaneous fat
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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Abstract
Description
본 발명은 솜나물 추출물을 함유하는 피하지방 감소 또는 손실 억제용 화장품 조성물에 관한 것이다.The present invention relates to a cosmetic composition for suppressing subcutaneous fat loss or loss containing a bittern root extract.
피하지방 조직은 피부의 볼륨과 강도를 좌우하는 역할을 하며 특히 얼굴 부위의 피부는, 노화가 진행됨에 따라 피하지방의 두께가 감소하여 주름이나, 탄력저하의 원인이 된다 (Fenske et al., J Am Acad Dermatol. 1986, 15:571-585; Rho et al., Bioorg Med Chem Lett. 2014, 24(9):2141-2145.). 그리고, 폐경기 여성에게 나타나는 호르몬, 혈액, 지방, 뼈 등의 대사 이상으로 인한 체내 지방의 재분포는 피부미용에 부정적으로 작용하고, 대사성 질환의 발병율을 높인다. Subcutaneous adipose tissue plays a role in the volume and intensity of skin, and in particular, the skin of the face decreases in thickness of subcutaneous fat as the aging progresses, which causes wrinkles and elasticity decrease (Fenske et al., J Am Acad Dermatol 1986, 15: 571-585; Rho et al., Bioorg Med Chem Lett., 2014, 24 (9): 2141-2145.). And, redistribution of body fat due to metabolic abnormalities such as hormones, blood, fat and bones appearing in postmenopausal women negatively affects skin beauty and increases the incidence of metabolic diseases.
최근, 지방세포를 피하에 이식하는 성형시술이 광범위하게 진행되고 있다. 특히 리포필링(lipofilling)으로 불리기도 하는 지방 이식은 노화 피부의 주름 개선을 비롯하여, 코성형, 턱선 및 이마선의 교정, 화상이나 상처로 인하여 함몰된 피부, 암 절제 수술로 상실된 부분 등에 사용한다. 하지만 지방 이식 후 이식 부피의 40~60%가 소실되는 문제가 있다 (S Eremia et al., 2000, Dermatol. Sur. 26, 1150-1158; Fulton JE et al., 2001, Dermatol. Clin. 19(3), 523-530). Recently, plastic surgery for transplanting adipocytes into the skin has been widely carried out. Fat transplantation, which is sometimes referred to as lipofilling, is used for wrinkles of aging skin, correction of nose, chin and forehead lines, depressed skin due to burns or wounds, and areas lost by cancer resection surgery. However, there is a problem in that 40-60% of the transplant volume is lost after transplantation (S Eremia et al., 2000, Dermatol. Sur. 26, 1150-1158; Fulton JE et al., 2001, Dermatol. 3), 523-530).
따라서 피부미용관점에서는 피하지방을 이식할 때, 이식되는 지방조직을 유지하거나 증가시킬 수 있는 방법이 필요하다. 또한 이식이 아닌 다른 방법으로 지방세포를 증가시켜 피하지방층을 증가시킴으로써 피하지방이 감소되는 것을 억제하는 것이 필요하다. 이러한 피하지방층 증가를 주름개선 및 피부의 처짐 방지 등의 미용성형 분야에 유용하게 사용될 수 있다 (Hu S et al., Ann Plast Surg. 2007 Jun;58(6):602-607; Yoshimura K et al., Aesthetic Plast Surg. 2008 ;32(1):48-55). 최근 피부 진피와 피하지방의 지방조직이 외부 세균이나 피부 상재균으로부터 피부를 보호하는 자연면역(natural immunity, innate immunity)에 중요한 역할을 한다는 사실이 알려져 있다 (Zhang et al., Science. 2015, 347:67-71). Therefore, from the viewpoint of skin beauty, when the subcutaneous fat is implanted, a method of maintaining or increasing the transplantation fat tissue is needed. It is also necessary to inhibit the reduction of subcutaneous fat by increasing the fat cells by increasing the subcutaneous fat layer in a manner other than transplantation. Such an increase in the subcutaneous fat layer can be usefully used in beauty cosmetic fields such as wrinkle improvement and prevention of skin sagging (Hu S et al., Ann Plast Surg 2007 Jun; 58 (6): 602-607; Yoshimura K et al , Aesthetic Plast Surg. 2008; 32 (1): 48-55). Recently, it has been known that adipose tissue of skin dermis and subcutaneous fat plays an important role in natural immunity (innate immunity) to protect skin from external bacteria or skin ulcers (Zhang et al., Science, 2015, 347 : 67-71).
따라서 피하지방조직의 유지 및 보호는 피부미용 측면 뿐 아니라 피부면역기능에도 매우 중요하다.Therefore, maintenance and protection of subcutaneous adipose tissue is very important not only in terms of skin beauty but also in skin immune function.
한편, 현재까지 주름개선이나 피부의 탄력 증진을 기대하는 화장품은 진피의 콜라겐을 증가시키거나, 콜라겐을 분해하는 효소를 억제하거나, 세포외기질(extracellular matrix)의 항상성을 조절하는데 초점을 둔 것들이 대부분이다. On the other hand, cosmetics that are expected to improve wrinkles and skin elasticity to date have focused on increasing dermal collagen, inhibiting collagen-degrading enzymes, or regulating the homeostasis of the extracellular matrix to be.
그러나, 피부의 기본적인 구조가 피하지방조직의 부피에 의해 유지되는 것임을 고려할 때, 지방 조직의 볼륨을 증가시키는 것이 피부의 볼륨 및 탄력을 유지하는 또 다른 해결책이 될 수 있을 것이다. 최근 지방세포에서 주로 만들어지는 사이토카인(cytokine)인 아디포넥틴(adiponectin)이 진피 섬유아세포의 콜라겐과 ECM의 생합성을 촉진시킨다는 보고가 있다 (Ezure and Amano. Biofactors. 2007, 31:229-236). 피하지방의 경우, 복부나 내장지방과는 달리 제2형 당뇨병의 인슐린 저항성과는 무관하다는 증거들이 최근 제시되고 있다 (Cnop et al., Diabetes. 2002, 51(4):1005-1015). However, considering that the basic structure of the skin is maintained by the volume of subcutaneous adipose tissue, increasing the volume of adipose tissue may be another solution to maintain the volume and elasticity of the skin. Recently, adiponectin, a cytokine mainly produced in adipocytes, has been reported to promote collagen and ECM biosynthesis of dermal fibroblasts (Ezure and Amano. Biofactors 2007, 31: 229-236). In the subcutaneous fat, the evidence suggests that unlike abdominal or visceral fat, it is not related to the insulin resistance of type 2 diabetes (Cnop et al., Diabetes 2002, 51 (4): 1005-1015).
즉, 피하지방의 증가는 인슐린감수성 측면에서 복부지방이나 내장지방에 비교하였을 때 피부 미용 측면에서의 장점과 피부의 자연면역 관점에서의 장점이 있다. 그러므로, 피하지방을 증가시키거나 아디포넥틴 생합성을 증가시키는 조성물은 노화를 방지하는 화장품, 피부미용, 피부성형보형물의 보조제 등으로 유용하게 사용될 수 있을 것이다. That is, the increase of subcutaneous fat is advantageous in terms of skin beauty compared with abdominal fat or visceral fat in view of insulin sensitivity and natural immunity of skin. Therefore, a composition that increases subcutaneous fat or increases adiponectin biosynthesis may be usefully used as cosmetics for preventing aging, skin cosmetics, adjuvants for skin molding implants, and the like.
대한민국 등록특허 제1609345호에는 계혈등, 고량강, 독활 및 천련자 추출물 중 적어도 1종 이상을 포함하는 인간 중간엽줄기세포의 지방세포로의 분화 촉진용 조성물이 개시되어 있으며, 대한민국 공개특허 제2012-0026689호에는 애엽 추출물을 포함하는 인간 중간엽줄기세포의 지방세포로의 분화 촉진용 조성물이 개시되어 있다.Korean Patent No. 1609345 discloses a composition for promoting the differentiation of human mesenchymal stem cells into adipocytes comprising at least one of cerebrospinal fluid, 0026689 discloses a composition for promoting the differentiation of human mesenchymal stem cells into adipocytes, comprising a lobule extract.
하지만 솜나물 또는 이의 추출물의 지방세포 분화 촉진에 대한 것은 전혀 개시되어 있지 않다. However, nothing has been disclosed about promoting adipocyte differentiation of wheat sprout or its extract.
본원은 솜나물 추출물을 함유하는 피하지방 감소 개선용, 즉 피하지방이 감소하는 것을 지연시키거나 또는 억제하는 화장품 조성물을 제공하고자 한다.The present invention seeks to provide a cosmetic composition for improving the reduction of subcutaneous fat, i.e., delaying or suppressing the decrease of subcutaneous fat, containing the extract of the wheat sprouts.
한 양태에서 본원은 솜나물 추출물을 포함하는 피하지방조직의 피하지방 감소 억제용 화장품 조성물을 제공한다. In one embodiment, the present invention provides a cosmetic composition for suppressing hypothecia reduction of subcutaneous fat tissue containing a spinach extract.
본원에 따른 조성물에 포함되는 추출물은 유기용매 추출물, 특히 에탄올 추출물이다. The extracts included in the compositions according to the present invention are organic solvent extracts, in particular ethanol extracts.
본원에 따른 추출물 또는 이를 포함하는 조성물은 피하지방 조직의 피하지방 감소를 억제하여, 콜라겐 생성 촉진 등을 통해 피부 주름, 특히 노화에 의한 주름개선, 탄력 증가와 같은 피부케어용 화장품에 유용하게 사용될 수 있다. The extract according to the present invention or a composition containing the same can be effectively used in skin care cosmetics such as skin wrinkles, especially wrinkles due to aging, and increase in elasticity, by suppressing the subcutaneous fat reduction of subcutaneous fat tissues and promoting collagen production have.
이런 측면에서 본원은 다른 양태에서 솜나물 추출물을 포함하는 피부케어 화장품 조성물을 제공한다. In this aspect, the present invention provides a skin care cosmetic composition comprising a somnambula extract in another embodiment.
다른 양태에서 본원은 또한 솜나물 추출물을 포함하는 콜라겐 생성 촉진용 화장품 조성물을 제공한다. In another embodiment, the present invention also provides a cosmetic composition for promoting collagen production, comprising a spinach extract.
또 다른 양태에서 본원은 또한 솜나물 추출물을 포함하는 피부 주름 개선용 화장품 조성물을 제공한다. In another aspect, the present invention also provides a cosmetic composition for improving skin wrinkles, comprising a wormwood extract.
본원에 따른 추출물 또는 조성물에 의한 피하지방 조직의 피하지방 감소 억제는 피하지방 조직의 중간엽 줄기세포의 지방세포로의 분화 촉진에 의한 것으로 이런 측면에서 본원은 또한 본원은 솜나물 추출물을 포함하는 피하지방 조직의 중간엽 줄기세포의 지방세포로의 분화 촉진용 화장품 조성물을 제공한다. Inhibition of subcutaneous fat reduction of subcutaneous fat tissue by the extract or composition according to the present invention is due to promotion of differentiation of mesenchymal stem cells of subcutaneous adipose tissue into adipocyte differentiation. In this respect, the present invention also relates to a subcutaneous adipose tissue The present invention provides a cosmetic composition for promoting the differentiation of mesenchymal stem cells into adipocytes.
본원에 따른 추출물은 또한 상술한 바와 같은 다용한 용도를 위해 건강기능 식품으로 제공될 수 있다.Extracts according to the present invention may also be provided as a health functional food for multi-purpose applications as described above.
이런 측면에서 본원은 솜나물 추출물을 포함하는 콜라겐 생성 촉진용 식품 조성물을 제공한다. In this respect, the present invention provides a food composition for accelerating collagen production, which comprises a wheat sprout extract.
또 다른 측면에서 본원은 솜나물 추출물을 포함하는 피하지방 조직의 중간엽 줄기세포의 지방세포로의 분화 촉진용 키트로 이러한 키트는 상기 목적을 위해 다양한 용도로 특히 인비트로에서 사용될 수 있다. In another aspect, the present invention relates to a kit for promoting the differentiation of mesenchymal stem cells into subcutaneous adipose tissue of subcutaneous adipose tissue, which comprises a spinach extract. The kit may be used for various purposes, particularly in Invitro, for the above purpose.
본 발명의 솜나물 추출물을 함유하는 피하지방 감소 억제용 화장품 조성물은 피하지방조직의 지방세포 분화를 촉진시키고 아디포넥틴을 증가시키는 효과를 나타낸다. The cosmetic composition for suppressing subcutaneous fat suppression containing the extract of Bombyx mori of the present invention has an effect of promoting adipocyte differentiation of subcutaneous fat tissue and increasing adiponectin.
따라서 상기 조성물은 증가된 지방세포 그리고 이로부터 분비되는 아디포넥틴에 의한 효과가 필요한 다양한 화장품 조성물로 유용하게 사용될 수 있다. 콜라겐 합성이 촉진되고, 피하지방층을 구성하거나, 피부노화에 따라 감소하는 지방세포를 유지함으로써 피부의 탄력을 늘리고 볼륨감을 주므로 피부지방 감소 개선 등의 피부미용 용도로 사용할 수 있다. 또한 피하지방층을 증가시키므로 생활습관 또는 노화 등으로 인한 주름 억제 또는 개선, 피부탄력 증가 또는 개선, 또는 피부의 처짐 방지 등과 같은 미용 성형에 효과적으로 사용될 수 있다. Therefore, the composition can be usefully used in various cosmetic compositions requiring increased fat cells and effects of adiponectin secreted therefrom. Collagen synthesis is promoted, subcutaneous fat layer is formed, or fat cells decreasing with aging of the skin are maintained, thereby increasing the elasticity of the skin and providing a volume sensation. Furthermore, since the subcutaneous fat layer is increased, it can be effectively used for cosmetic molding such as suppression or improvement of wrinkles due to lifestyle or aging, increase or improvement of skin elasticity, prevention of sagging of skin, and the like.
상기 지방세포 분화 촉진 효과는 특정 부위의 지방조직에 한정되는 것이 아니며 예를 들어 지방층이 많은 안면부위는 물론, 여성의 가슴 부위 또는 둔부, 손등, 발등에도 적용가능하다.The effect of promoting differentiation of adipocytes is not limited to fatty tissue of a specific site. For example, the present invention is applicable not only to a face region having many fat layers, but also to a breast region, a buttock, a back and a foot.
도 1은 본원의 일 구현예에 따른 솜나물 추출물에 의한 hAT-MSC의 지방분화 촉진 효과를 나타낸 것이다. 오일레드오(Oil Red O) 염색으로 hAT-MSC 지방분화 촉진 효과를 평가하였다.
도 2는 본원의 일 구현예에 따른 솜나물 추출물에 의한 hAT-MSC의 아디포넥틴 생합성 촉진 효과를 나타낸 것이다.
도 3은 본원의 일 구현예에 따른 솜나물 추출물에 의한 HDEs에서의 콜라겐 합성 촉진 효과를 나타낸 것이다. FIG. 1 shows the effect of promoting fat differentiation of hAT-MSC by the extract of pine sprouts according to one embodiment of the present invention. The effect of promoting hAT-MSC lipid differentiation with Oil Red O staining was evaluated.
FIG. 2 shows the effect of hAT-MSC on the promotion of adiponectin biosynthesis by the extract of pine nuts according to one embodiment of the present invention.
FIG. 3 is a graph showing the effect of accelerating collagen synthesis in HDEs by the extract of pine needles according to one embodiment of the present invention.
본원은 생체 내 독성이 없는 천연물을 연구하고 검색하던 중 솜나물 추출물이 중간엽줄기세포가 지방세포로 분화하는 것을 촉진하고, 이에 따라 아디포넥틴이 증가시킨다는 발견에 기초한 것이다. The present invention is based on the discovery that somnal extract promotes the differentiation of mesenchymal stem cells into adipocytes and thus increases adiponectin, while studying and searching for natural non-toxic natural products.
이에 한 양태에서 본원은 솜나물 추출물을 포함하는 피하지방조직에 존재하는 피하지방 감소 개선 또는 억제용 화장품 조성물에 관한 것이다.In one aspect, the present invention relates to a cosmetic composition for improving or inhibiting subcutaneous fat reduction present in subcutaneous fat tissue containing a spinach extract.
피하지방조직은 진피와 근막 사이에 위치하며 진피와 근막 사이를 느슨하게 결합시켜 주는 결합조직을 말하며 주로 지방세포로 구성된다. 피하지방조직에서 지방세포 분화가 촉진되면 피하지방조직 층을 구성하는 지방세포가 증가되어 피부노화에 따라 감소하는 지방세포 수를 보충하고 일정 숫자로 유지 또는 증가하게 한다. 또한 상기 증가된 지방세포로부터 방출되는 물질로 인해 피부의 탄력을 증가시키게 하고 피부 볼륨감을 주면서, 피부 주름이 억제되고, 궁극적으로 피부노화가 개선된다. Subcutaneous adipose tissue is located between the dermis and fascia, and is a connective tissue that loosely bonds between the dermis and fascia, and is composed mainly of adipocytes. When the adipocyte differentiation is promoted in the subcutaneous adipose tissue, the adipocyte constituting the subcutaneous adipose tissue layer is increased to supplement or reduce the number of adipocytes that decrease with aging of the skin to a certain number. In addition, the substances released from the increased fat cells increase the elasticity of the skin and give the skin a feeling of volume, while suppressing the wrinkles of the skin and ultimately improving the aging of the skin.
본원의 솜나물 추출물은 중간엽 줄기세포로부터 지방세포로의 분화를 촉진하게 한다. The wheat extract of the present invention promotes differentiation from mesenchymal stem cells into adipocytes.
본원에 따른 조성물에 포함되는 추출물은 솜나물(Leibnitzia anandria) 유래이다. 솜나물은 쌍떡잎식물로 초롱꽃목 국화과의 여러해살이풀이다. 그늘진 곳에서 잘 자란다. 잎은 계란 꼴에 가까운 길쭉한 타원꼴로 가장자리에는 작은 톱니와 같은 결각이 있다. 잎 사이로 30cm 정도의 꽃대가 각기 1송이씩 꽃을 가진다. 꽃의 지름은 1.5cm 안팎으로 흰빛이다 (「몸에 좋은 산야초」, 2009. 11. 15., (주)넥서스). 어린 순을 나물로 먹는다. 祛風濕(거풍습), 해독의 효능이 있고 류머티즘에 의한 마비, 咳喘(해천), 정창을 치료한다 (이미주, 2010, “한국의 야생화, 야생화 찾기 - 솜나물”, http://www.yasangwha.co.kr/). The extracts contained in the composition according to the present application are derived from the spiny plant (Leibnitzia anandria). Spiny sprout is a perennial plant of dicotyledon. It grows well in shady places. Leaves are elongated elliptical eggs close to egg-shaped, with small sawtooth-shaped incisions on the edges. Each leaf has about 30cm of flowers between each leaf. The diameter of the flower is about 1.5cm in white ("Sanayasu for the body", Nov. 15, 2009, Nexus Co., Ltd.). I eat young seeds as herbs. It treats 祛风), detoxification efficacy, paralysis by rheumatism, cough asthma, and sunburn (Alienism, 2010, "Korean Wildflower, Wildflower Seeker - Something", http: // www. yasangwha.co.kr/).
본원에 사용되는 솜나물은 줄기가 주로 사용되나, 생리활성을 포함하는 한, 종피, 잎, 줄기, 뿌리, 종자 및 열매가 또한 사용될 수 있다. As used herein, stalks are mainly used for stalks, but may include seeds, leaves, stems, roots, seeds and fruits, as long as they contain physiological activity.
본 명세서에서 사용된 용어 "추출물(extract)"이란 천연물로부터 분리된 활성성분, 즉 목적하는 활성을 보이는 물질을 의미한다. 상기 추출물은 물, 또는 적절한 유기용매 예를 들어, 메탄올, 에탄올 등, 또는 이들의 혼합용매를 이용하는 추출과정으로 획득할 수 있으며, 추출물, 이의 건조 분말 또는 이를 이용하여 제형화된 모든 형태를 포함한다. 또한 상기 추출물에는 상기 추출과정을 거친 추출물을 분획한 것도 포함된다. As used herein, the term "extract" means an active ingredient separated from a natural product, i.e., a substance exhibiting the desired activity. The extract may be obtained by an extraction process using water or an appropriate organic solvent such as methanol, ethanol, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or any form thereof formulated with the extract . The above-mentioned extract also includes an extract obtained by fractionating the above extract.
상기 용매는 물, 유기용매 또는 이들의 혼합용매일 수 있다. 이로 제한하는 것은 아니나, 구체적으로는 물, 메탄올, 에탄올, 부탄올, 프로판올 및 이소프로판올 등을 포함하는, 탄소수 1 내지 5의 저가 알코올, 에틸아세테이트 등의 극성용매와 헥산 또는 디클로로메탄의 비극성용매와 같은 유기용매 또는 이들의 혼합용매일 수 있고, 특히 물, 탄소수 1 내지 4의 알코올 또는 이들의 혼합용매일 수 있으며, 더욱 특히는 물, 50 내지 100%의 메탄올, 에탄올 등의 탄소수 1 내지 4의 알코올일 수 있다. 본원에 따른 일 구현예에서는 유기용매, 특히 에탄올이 용매로 사용되어 우수한 효과를 나타낸다. The solvent may be water, an organic solvent or a mixture thereof. But are not limited to, water, methanol, ethanol, butanol, propanol, and isopropanol, a lower alcohol having 1 to 5 carbon atoms, a polar solvent such as ethyl acetate, and an organic solvent such as hexane or dichloromethane A solvent, or a mixture thereof. In particular, it may be water, an alcohol having 1 to 4 carbon atoms or a mixture thereof, more particularly water, an alcohol having 1 to 4 carbon atoms such as 50 to 100% . In one embodiment according to the present invention, an organic solvent, particularly ethanol, is used as a solvent to exhibit an excellent effect.
본원의 추출물은 통상의 식물 추출물의 공지된 일반적 제조방법을 사용하여 제조될 수 있으며, 특별히 제한되지 않는다. 예를 들면, 초음파 추출법, 환류 냉각 추출법, 열수 추출법 또는 상기 용매를 추출용매로 하여 공지의 방법에 의해 제조하는 방법일 수 있다 (예를 들어 Nomura T. Phenolic compounds of the mulberry tree and related plants. In: Progress in the Chemistry of Organic Natural Products (Herz W, Grisebach H, Kirby GW, Tamm Ch, eds.), vol. 53. Springe:Vienna, 1988;87-201). 또한 추출과정을 거친 추출물은 이후, 헥산, 메틸렌클로라이드, 아세톤, 에틸아세테이트, 클로로포름 및 이들의 혼합물로 이루어진 군으로부터 선택된 용매로 분획과정을 더욱 실시할 수 있다. 상기 분획 시 용매는 2종 이상 개별적으로 또는 혼합하여 사용할 수 있으며, 용매의 극성에 따라 순차적으로 사용하여 각 용매의 추출물을 제조할 수 있다. 추출물 제조온도는 4 내지 120 ℃일 수 있으나, 이에 한정되지는 않는다. 추출시간은 특별히 한정되지는 않으나 10분 내지 30일 일 수 있으며, 통상의 추출기기, 초음파분쇄 추출기 또는 분획기를 이용할 수 있다. 제조된 추출물은 이후 감압 여과 또는/및 동결건조하여 용매를 제거할 수 있다. 예를 들면, 솜나물에 용매를 1:1 내지 1:20 중량비로 가열 순환식 추출탱크에 넣고, 정제수 또는 식용 알콜을 가하여 80 내지 100℃에서 6-24시간 정도 추출한 후 여과하여 여액을 얻고, 잔여물에 다시 정제수 또는 식용 알콜을 가하여 동일한 과정을 수회 반복할 수 있다. 여액을 합하여, 그 자체로 사용하거나, 이를 진공 농축기로 감압하에서 농축한 후, 열풍 또는 동결 건조하여 얻은 건조물을 분쇄기로 분쇄하여 사용할 수 있다. The extract of the present invention can be produced by using a common general production method of a conventional plant extract, and is not particularly limited. For example, it may be an ultrasonic extraction method, a reflux cooling extraction method, a hot water extraction method, or a method in which the above solvent is used as an extraction solvent by a known method (for example, Nomura T. Phenolic compounds of the mulberry tree and related plants. : Progress in the Chemistry of Organic Natural Products (Herz W, Grisebach H, Kirby GW, Tamm Ch, eds.), 53. Springe: Vienna, 1988: 87-201). Further, the extracted extract can be further fractionated with a solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, chloroform, and mixtures thereof. Two or more kinds of solvents may be used in the fractionation step, or they may be used individually or in combination. Depending on the polarity of the solvent, an extract of each solvent may be sequentially used. The extract preparation temperature may be 4 to 120 ° C, but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 30 days, and conventional extraction equipment, ultrasonic pulverization extractor or fractionation unit may be used. The produced extract can then be subjected to vacuum filtration and / or lyophilization to remove the solvent. For example, the solvent is added to the spinach at a weight ratio of 1: 1 to 1:20 by weight in a heating / circulating extraction tank, purified water or edible alcohol is added and the mixture is extracted at about 80 to 100 DEG C for about 6 to about 24 hours and then filtered to obtain a filtrate. The same procedure can be repeated several times by adding purified water or edible alcohol to the water. The filtrate may be used as it is, or it may be concentrated under reduced pressure with a vacuum concentrator, and then the dried product obtained by hot air or lyophilization may be pulverized by a pulverizer.
본원의 일 구현예에서, 본 발명의 솜나물 추출물은, 재료 약 30g당 약 95% 에탄올 10 내지 500ml를 가한 후 추출하는 방법으로 실시된다. 추출 온도는 약 30 내지 150℃일 수 있으나, 이에 한정되진 않는다. 추출시간은 한정되진 않으나 30분 내지 수일 일 수 있으며, 통상의 추출 기기나 초음파분쇄 추출기를 이용할 수 있다. 제조된 추출물은 이후 감압 여과 및 건조하여 사용될 수 있다. 본원에서 건조공정은 동결건조, 진공건조, 열풍건조, 분무건조, 감압건조, 포말건조, 고주파건조 또는 적외선건조 등을 포함하는 방법으로 수행할 수 있으나, 이에 제한되지 않는다.In one embodiment of the invention, the pombalum extract of the present invention is prepared by adding 10 to 500 ml of about 95% ethanol per about 30 g of the material, followed by extraction. The extraction temperature may be about 30 to 150 캜, but is not limited thereto. The extraction time is not limited, but may be 30 minutes to several days, and an ordinary extraction device or an ultrasonic pulverizing extractor may be used. The prepared extract can then be used by vacuum filtration and drying. The drying process in the present invention may be performed by a method including, but not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying or infrared drying.
본원에 따른 일 구현예에서 본원의 솜나물 추출물에 의하여 상기 중간엽줄기세포로부터 지방세포로의 분화가 촉진되고 이에 따라 아디포넥틴 분비가 증가하고, 지방조직도 증가함으로써 피하지방 감소 개선이 이루어진다. In one embodiment of the present invention, the extract of the present invention promotes differentiation from mesenchymal stem cells into adipocytes, thereby increasing adiponectin secretion and increasing fat tissue, thereby improving subcutaneous fat reduction.
본 명세서에서 사용되는 용어 "중간엽줄기세포"는 골수(bone marrow), 혈액, 진피 또는 골막에서 분리되는 줄기세포로서, 다양한 세포 예컨대 지방세포, 연골세포 및 뼈세포 등으로 분화할 수 있는 다능성 세포로, 특히 진피, 피하지방조직에 존재하는 세포를 포함한다.As used herein, the term "mesenchymal stem cell" is a stem cell which is isolated from bone marrow, blood, dermis or periosteum. It is a pluripotent stem cell capable of differentiating into various cells such as adipocytes, chondrocytes, Cells, in particular dermal and subcutaneous fat tissue.
상기 중간엽줄기세포는 동물, 바람직하게는 포유동물, 보다 바람직하게는 인간 유래이다. The mesenchymal stem cells are derived from an animal, preferably a mammal, more preferably a human.
본 명세서에 사용된 상기 "아디포넥틴"은 지방 세포 분화 과정 중 지방세포에서 분비되는 대표적인 아디포카인(adipokine) 중 하나이다.As used herein, the term "adiponectin" is one of the representative adipokines secreted by adipocytes during adipocyte differentiation.
한편, 다른 일 구현예에서 본원의 상기 추출물은 콜라겐 합성을 촉진시킨다. 상기 콜라겐은 피부, 혈관, 뼈, 치아, 근육 등 모든 결합조직의 주된 단백질이다. 종래에는 콜라겐의 피부 보습 효과 및 상처 치유 효과를 이용하기 위하여 화장품 또는 연고 등과 같은 피부 외용제 조성물에 콜라겐을 배합하였으나, 콜라겐 자체의 경피 흡수가 어려워 상기 효과를 기대하기 어렵다. 하지만 본원의 상기 추출물과 같이 진피 등과 같은 조직에서 콜라겐 합성을 촉진시키면 인체에 안정하면서도 콜라겐의 효과를 기대할 수 있다. In another embodiment, the extract of the present invention promotes collagen synthesis. The collagen is the main protein of all connective tissues such as skin, blood vessels, bones, teeth, muscles. Conventionally, in order to utilize the skin moisturizing effect and the wound healing effect of collagen, collagen is added to a composition for external application for skin such as cosmetics or ointment, but it is difficult to absorb the percutaneous absorption of collagen itself. However, when the collagen synthesis is promoted in tissues such as dermis as in the extract of the present invention, the effect of collagen can be expected while being stable to human body.
즉, 본원에 따른 화장품 조성물은 지방조직을 이루는 지방세포의 분화를 촉진하고 아디포넥틴의 생합성을 증가시킴으로써 근본적으로 피부의 볼륨감을 증가시켜 줄 수 있고, 또한 콜라겐의 합성을 증가시켜, 결과적으로 피부의 탄력을 지속적으로 유지하고 주름을 개선시킬 수 있다. That is, the cosmetic composition according to the present invention can promote the differentiation of adipocytes constituting adipose tissue and increase the biosynthesis of adiponectin, which can basically increase the volume feeling of the skin, increase the synthesis of collagen, Can be maintained and wrinkles can be improved.
이런 측면에서 본원은 솜나물 추출물을 포함하는 콜라겐 합성 촉진용 화장품 조성물로 제공될 수 있다. In this respect, the present invention can be provided as a cosmetic composition for accelerating collagen synthesis comprising a pine needle extract.
이런 측면에서 본원에 따른 조성물은 피부탄력 개선 또는 증가; 또는 피부 주름 개선 또는 주름 억제용 화장품 조성물로 제공될 수 있다.In this respect, the compositions according to the present invention are useful for improving or increasing skin elasticity; Or as a cosmetic composition for wrinkle reduction or wrinkle suppression.
이런 측면에서 본원에 따른 조성물은 피부케어 화장품 조성물로 제공될 수 있다. 이러한 피부케어 화장품 조성물은 특히, 습관 또는 노화에 의한 주름 억제, 개선, 피부 탄력 증가 또는 개선을 목적으로 사용될 수 있다. In this respect, the composition according to the present invention may be provided as a skin care cosmetic composition. Such skin care cosmetic compositions can be used particularly for the purpose of suppressing wrinkles caused by habit or aging, improving skin elasticity, or improving skin elasticity.
따라서 본원에 따른 솜나물 추출물을 포함하는 화장품용 조성물은 미용 또는 성형 목적으로 사용될 수 있다. Therefore, the cosmetic composition containing the extract of the wheat sprouts according to the present invention can be used for cosmetic or cosmetic purposes.
본원에서 사용된 용어 ‘화장품’은 인체를 청결미화하여 용모를 밝게 변화시키거나 피부, 모발의 건강을 유지 또는 증진하기 위하여 인체에 사용되는 물품을 의미하며, 인체에 대한 작용이 경미한 것을 말한다. 통상적인 의미로서, 로션, 크림, 오일, 세정용 제품 및 기능성 화장품을 모두 포함한다. 기능성 화장품이란 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 화장품의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 화장품군이나 화장품 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 화장품을 의미한다. As used herein, the term 'cosmetics' refers to an article used in the human body to change the appearance of a human body to brighten its appearance or to maintain or promote the health of skin and hair, and to have a slight effect on the human body. Commonly used include lotions, creams, oils, cleansing products, and functional cosmetics. Functional cosmetics are physical control, biochemical, and biotechnological techniques that control the body's defense rhythm, cosmetic control, disease control and recovery, Means a cosmetic product which is designed and manufactured so that its function is sufficiently expressed in living bodies.
본원에 따른 화장품 조성물은 화장품, 예를 들면, 크림, 로션, 앰플, 스킨, 에센스, 비누 등으로 제공될 수 있으며, 다른 측면에서 기초 화장품 조성물(화장수, 크림, 에센스, 클렌징 폼 및 클렌징 워터와 같은 세안제, 팩, 보디오일), 색조 화장품 조성물(화운데이션, 립스틱, 마스카라, 메이크업 베이스), 또는 마스크 또는 팩의 형태로 제조될 수 있다.The cosmetic composition according to the present invention may be provided as a cosmetic product such as a cream, a lotion, an ampoule, a skin, an essence, a soap, (Liposome, lipstick, mascara, makeup base), or a mask or pack.
본원에 따른 화장품 조성물이 적용될 수 있는 부위는 신체의 특정 지방 조직에 한정되는 것은 아니며 예를 들면, 지방층이 많은 여성의 가슴 부위, 손등, 발등 및 둔부를 포함할 수 있다. 적용되는 부위에 다양한 투여 경로, 예를 들면 전신 또는 국소, 특히 국소 투여, 경피, 또는 주사, 특히 경피 경로로 투여될 수 있다.The site to which the cosmetic composition according to the present application may be applied is not limited to a specific fat tissue of the body, but may include, for example, a breast region of a woman with a lot of fat layers, a back, a foot and a buttock. The site of application may be administered by a variety of routes of administration, such as systemic or topical, especially topical, transdermal, or injection, particularly the transdermal route.
본원에 따른 화장품 조성물은 지시된 비율로 필수 성분으로서 본원의 솜나물 추출물을 포함하는 외에는 다른 성분에는 특별한 제한이 없으며 통상의 화장품, 예를 들면 크림의 경우, 식물성 오일, 유화제, 증점제, 향료, 물, 산화방지제, 및 UV 흡수제를 포함 할 수 있다.The cosmetic composition according to the present invention has no particular limitation on the other components other than the swell extract of the present invention as an essential ingredient in the indicated ratios, and can be used in the case of ordinary cosmetics such as cream, vegetable oils, emulsifiers, thickeners, Antioxidants, and UV absorbers.
본원에 따른 조성물에 포함되는 추출물의 함량은 목적하는 제품의 구체적 용도 또는 제형 등에 따라 달라질 수 있으나, 전체 조성물 중에 약 0.001 내지 약 50 중량% 로 포함될 수 있으나, 이 범위를 벋어나는 것을 제외하는 것은 아니다. The amount of the extract contained in the composition according to the present invention may vary depending on the specific application or formulation of the desired product, but it may be included in the total composition in an amount of about 0.001 to about 50% by weight, but not limited to the range .
본원의 추출물을 포함하는 화장용 조성물은 상기 추출물 이외에 항산화제, 안정화제, 용해화제, 비타민, 안료 및/또는 향료와 같은 통상적인 보조제 및 담체를 포함할 수 있다. The cosmetic composition comprising the extract of the present invention may contain, in addition to the above extracts, conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and / or flavors.
본원의 화장용 조성물은 공액리놀레산, 리놀레산, 감마-리놀레산, 알파-리놀레산을 포함하는 불포화지방산 또는 프로필렌 글리콜을 포함하는 수용성 기제 물질을 포함할 수 있다. The cosmetic composition of the present invention may comprise a water-soluble base material comprising an unsaturated fatty acid or propylene glycol including conjugated linoleic acid, linoleic acid, gamma-linoleic acid, alpha-linoleic acid.
또한 본원의 화장용 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 유연 화장수, 영양 화장수, 영양 크림, 마사지 크림, 에센스, 아이크림, 클렌징 크림, 클렌징 폼, 클렌징 워터, 마스크, 팩, 스프레이 또는 파우더의 제형으로 제조될 수 있다.In addition, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, But are not limited to, cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask, a pack, a spray or a powder.
본원 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the composition of the present invention is a paste, cream or gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide may be used as a carrier component .
본원 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the composition of the present composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
본원에 따른 화장품 조성물은 화장품용으로/피부학적으로 허용가능한 담체를 추가로 포함할 수 있다. "화장품용으로/피부학적으로 허용가능한"은 지나친 독성, 불상용성, 불안정성, 자극, 알러지 반응 등이 없이 조직(예를 들어, 피부 또는 모발)과 접촉하여 사용하기에 적합함을 의미한다.The cosmetic composition according to the present invention may further comprise a cosmetically / dermatologically acceptable carrier. "Cosmetically / dermatologically acceptable" means suitable for use in contact with a tissue (e.g., skin or hair) without undue toxicity, insolubility, instability, irritation, allergic response,
본원 조성물의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 사용될 수 있다. When the formulation of the composition is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.
본원 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소 결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 사용될 수 있다.When the formulation of the present composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
본원 조성물의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리움 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다. 바람직한 투여 유형에 따르면, 본 발명의 조성물은 최소한 하나의 약학적으로 허용되는 부형제, 특히 피부학적으로 허용 가능한 부형제를 포함할 수 있다. When the formulation of the composition of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolium derivatives, methyltaurates, sarcosinates, fatty acid amide ethers Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester. According to a preferred mode of administration, the compositions of the present invention may comprise at least one pharmaceutically acceptable excipient, especially a dermatologically acceptable excipient.
또한, 상기 각 제형의 피부용 조성물에 있어서, 상기한 필수 성분 이외의 다른 성분들은 기타 피부용 조성물 또는 사용목적 등에 따라 당업자가 어려움 없이 선정하여 배합할 수 있다.Further, in the composition for dermatological preparation of each of the formulations, components other than the above-mentioned essential components may be selected and mixed without difficulty by those skilled in the art depending on other compositions for dermal use or the purpose of use.
본 발명의 조성물은 독성 및 부작용은 거의 없으므로 예방 목적으로 장기간 투여 시에도 안심하고 사용할 수 있다. Since the composition of the present invention has little toxicity and side effects, it can be safely used for prolonged administration for preventive purposes.
한편, 지방세포에서 아디포넥틴이 분비되는데 상기 아디포넥틴은 인슐린 감수성을 증가시켜 혈당을 낮추는 효과를 지닌다. 본원의 상기 솜나물 추출물은 지방세포 분비를 촉진시키고, 이에 따라 아디포넥틴을 증가시키므로, 2차 당뇨병 등과 같은 대사성 질환에 또한 유용하게 사용될 수 있다. On the other hand, adiponectin is secreted in adipocytes. Adiponectin has an effect of lowering blood glucose by increasing insulin sensitivity. The wormwood extract of the present invention promotes adipocyte secretion and thus increases adiponectin, and thus may be useful also for metabolic diseases such as secondary diabetes.
이에 다른 양태에서 본 발명은 솜나물 추출물을 함유하는 대사성 질환 예방 및 치료용 조성물 또는 대사성 질환의 개선을 위한 건강식품으로 제공될 수 있다. In another aspect thereof, the present invention can be provided as a composition for preventing and treating metabolic diseases or a health food for the improvement of metabolic diseases, which contains a Summa extract.
상기 대사성 질환은 비만, 제2형 당뇨병, 고지혈증, 고콜레스테롤증, 동맥경화증 및 지방간으로 이루어진 군에서 선택된 하나 이상의 질병을 말한다. The metabolic disease refers to one or more diseases selected from the group consisting of obesity, type 2 diabetes, hyperlipidemia, hypercholesterolemia, atherosclerosis and fatty liver.
본 발명에 따른 대사성 질환의 예방 또는 치료용 조성물은 '약학적으로 유효한 양'의 솜나물 추출물을 단독으로 포함하거나 하나 이상의 약학적으로 허용되는 담체, 부형제 또는 희석제를 포함할 수 있다. 상기에서 '약학적으로 유효한 양'이란 대사성 질환을 예방, 개선 및 치료하기에 충분한 양을 말한다. 본 발명에 따른 솜나물 추출물의 약학적으로 유효한 양은 0.5 ~ 100 mg/day/체중kg, 바람직하게는 0.5 ~ 5 mg/day/체중kg 일 수 있다.The composition for the prevention or treatment of metabolic diseases according to the present invention may contain a 'pharmaceutically effective amount' of the bittern extract alone or may comprise one or more pharmaceutically acceptable carriers, excipients or diluents. As used herein, the term " pharmaceutically effective amount " refers to an amount sufficient to prevent, ameliorate, and treat metabolic diseases. The pharmacologically effective amount of the swell extract according to the present invention may be 0.5-100 mg / day / kg body weight, preferably 0.5-5 mg / day / kg body weight.
또한 본 발명은 상기 기술한 본원의 추출물을 포함하는 조성물은 식품, 예를 들면, 건강보조식품, 기능성 식품, 식품첨가제 등으로 제공될 수 있다. In addition, the composition of the present invention comprising the extract of the present invention described above may be provided as a food, for example, a health supplement, a functional food, a food additive and the like.
본 명세서에서 사용된 용어 "식품"이란 영양소를 한 가지 또는 그 이상 함유하고 있는 천연물 또는 가공품을 의미하며, 바람직하게는 어느 정도의 가공 공정을 거쳐 직접 먹을 수 있는 상태가 된 것을 의미하며, 통상적인 의미로서, 식품, 식품 첨가제, 기능성식품 및 음료를 모두 포함하는 의도이다.As used herein, the term "food" means a natural or processed product containing one or more nutrients, preferably a state of being ready to be eaten through a certain degree of processing, It is intended to include food, food additives, functional foods and beverages as a meaning.
본 명세서에서 사용된 용어 "기능성식품"이란 식품에 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 식품의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 식품군이나 식품 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 식품을 의미하며, 특히 "건강기능식품"을 포함한다.As used herein, the term "functional food" refers to a food group that has been imparted with added value to function or express the function of the food by physical, biochemical, or biotechnological techniques, , And the body control function of disease prevention and recovery is designed to be fully expressed in the living body, and in particular, includes "health functional food".
본 명세서에서 사용된 용어 "건강보조식품"이란 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 가공한 식품으로서, 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻을 건강보조의 목적으로 특정성분을 원료로 하거나 식품원료에 들어있는 성분을 추출, 농축, 정제, 혼합 등의 방법으로 제조, 가공한 식품을 말한다. As used herein, the term "health supplement food" is a food prepared by using raw materials or ingredients having useful functions in the human body, and is useful for health and other purposes such as controlling nutrients and physiological actions on the structure and function of the human body For the purpose of health assisting to obtain the effect, it refers to a food which is made by processing a specific ingredient as a raw material or by extracting, concentrating, refining, mixing, etc. ingredients contained in a food raw material.
상기 기능성식품 및 건강보조식품에는 식품학적으로 허용 가능한 식품 보조 첨가제를 더욱 포함할 수 있으며, 기능성 식품의 제조에 통상적으로 사용되는 적절한 담체, 부형제 및 희석제를 더욱 포함할 수 있다.The functional food and the health supplement food may further include a food-acceptable food supplementary additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of a functional food.
본원의 추출물을 첨가할 수 있는 식품으로는 예를 들어, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 기능성 식품 등이 있다. 추가로, 본 발명에서 식품에는 특수영양식품(예, 조제유류, 영,유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예, 라면류, 국수류 등), 건강보조식품, 조미식품(예, 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예, 스넥류), 유가공품(예, 발효유, 치즈 등), 기타 가공식품, 김치, 절임식품(각종 김치류, 장아찌 등), 음료(예, 과실,채소류 음료, 두유류, 발효 음료류 등), 천연조미료(예, 라면 스프 등)을 포함하나 이에 한정되지 않는다. 상기 식품, 음료 또는 식품첨가제는 통상의 제조방법으로 제조될 수 있다.Foods to which the present extracts can be added include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, in the present invention, the food may contain special nutritional foods (e.g., crude oil, spirits, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, fruits, vegetables, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, beverage or food additive may be prepared by a conventional production method.
일 구현예에서는 기능성 음료로 제조되며, 기능성 음료란 음료에 물리적, 생화학적, 생물공학적 수법 등을 이용하여 해당 음료의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 음료 군이나 음료 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 음료를 의미하며, 특히 피부 탄력 향상, 콜라겐 합성 촉진을 위한 음료를 의미한다.In one embodiment, the functional beverage is made of a functional beverage. The functional beverage includes a beverage group to which the added value is imparted so as to function and express the function of the beverage to a specific purpose, physical or biochemical, Defensive rhythm control, disease prevention and recovery, etc., means a beverage which is designed to sufficiently express the body control function against the living body, and particularly refers to a drink for improving skin elasticity and promoting collagen synthesis.
본원의 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다. 이러한 성분을 독립적으로 또는 조합하여 사용할 수 있다. The food composition of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate, etc.), pectic acid and its salts, Organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. These components can be used independently or in combination.
이하, 본 발명의 이해를 돕기 위해서 실시예를 제시한다. 그러나 하기의 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐 본 발명이 하기의 실시예에 한정되는 것은 아니다.Hereinafter, embodiments are provided to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited to the following examples.
실시예 Example
실시예Example 1. 솜나물 추출물의 제조 1. Manufacture of Spinach Extract
솜나물 30g을 에탄올(95.0 ~ 95.5v/v%) 200ml에 넣은 다음 ASE300 ACCELERATED SOLVENT EXTRACOR(DIONEX Corporation) 기기를 이용하여 50도씨에서 1500psi 압력하에 20분간 분쇄하였다. 또한 Ultrasonic cleaner(BRANSON Ultrasonics corporation) 소니케이터 기기를 이용하여 50도씨에서 3일 정도 추가 분쇄하였다. 이렇게 분쇄된 솜나물 추출물을 Modulspin 40 (Biotron corporation) 기기를 이용하여 40도씨에서 24시간 동안 건조하였으며, 사용 전 70% 에탄올에 녹여서 사용하였다.30 g of wheat flour was placed in 200 ml of ethanol (95.0 to 95.5 v / v%) and pulverized for 20 minutes under a pressure of 1500 psi at 50 degrees Celsius using an ASE 300 ACCELERATED SOLVENT EXTRACOR (DIONEX Corporation). In addition, using an ultrasonic cleaner (BRANSON Ultrasonics corporation) sonicator device, it was further pulverized for 3 days at 50 ° C. The crushed wheat sprout extract was dried at 40 ° C for 24 hours using a Modulspin 40 (Biotron corporation) instrument and dissolved in 70% ethanol before use.
실시예 2. 솜나물 추출물에 의한 지방세포 분화 촉진 효능Example 2. Efficacy of promoting adipocyte differentiation by the extract of somnuta
실시예Example 2-1. 인간의 지방유래 2-1. Human origin 중간엽줄기세포Mesenchymal stem cells 배양 culture
인간의 지방유래 중간엽줄기세포(human adipose tissue derived mesenchymal stem cells, hAT-MSCs)(Lonza, Inc. Walkersville, MD, USA)는 제조사의 설명서에 따라 배양하였다. Human adipose tissue derived mesenchymal stem cells (hAT-MSCs) (Lonza, Inc., Walkersville, MD, USA) were cultured according to the manufacturer's instructions.
실시예Example 2-2. 솜나물 추출물의 지방세포 분화 촉진 효능 2-2. Efficacy of the extract of pine needles to promote adipocyte differentiation
hAT-MSC의 지방세포 분화는 인도메타신(indomethacin)을 제외한 채로 제조사사의 방법에 따라 유도하였다. 구체적으로, 중간엽줄기세포의 배양액에 인슐린 1 μg/ml, 덱사메타손(Dexamethasone, DEXA) 1 μM, 이소부틸메틸잔틴 (isobutylmethylxanthine, IBMX) 0.5 mM 을 넣고 배양하여 hAT-MSC를 지방세포로 분화시켰으며, 실험군의 경우 지방세포 분화 유도 시에 솜나물 추출물을 50 μg/ml 과 25 μg/ml 농도로 배지에 첨가하였다. 양성 대조군으로는 글리벤클라마이드(Glibenclamide) 10 μM 과 아스피린(Aspirin) 300 μM 을 각각 사용하여, IDX 배지와 같이 처치하였다. 중간엽줄기세포의 지방세포 분화를 시작한 지 14일 후, 세포 배양액을 제거하고, 10 ml의 인산염 완충액(Phosphate Buffered Saline, PBS)으로 세포를 세척한 후, 10% 포르말린/PBS 용액 0.2 ml/cm2을 사용하여 세포를 10분간 고정시켰다. 고정된 세포를 60% 이소프로판올 용액 0.5 ml/cm2로 1회 세척하고, 오일레드오(Oil Red O)를 60% 이소프로판올에 녹인 염색액 0.2 ml/cm2로 10분간 지방세포 내의 지질체를 염색하였다. 이어서, 헤마톡실린(hematoxylin)으로 재염색을 행하여 세포핵을 쉽게 구분되도록 한 후 현미경으로 관찰하였고 그 관찰 결과를 도 1에 나타내었다. 도 1에서 볼 수 있는 바와 같이, 솜나물 추출물은 중간엽줄기세포의 세포 사멸을 유도하지 않으면서 지방세포의 분화를 촉진하였다.Adipocyte differentiation of hAT-MSC was induced by the manufacturer's method with the exception of indomethacin. Specifically, hAT-MSC was differentiated into adipocytes by culturing the medium with insulin 1 μg / ml, dexamethasone (DEXA) 1 μM, and isobutylmethylxanthine (IBMX) 0.5 mM in the culture medium of mesenchymal stem cells . In the experimental group, when the adipocyte differentiation was induced, the somniferous extract was added to the medium at a concentration of 50 μg / ml and 25 μg / ml. Glucenclamide (10 μM) and aspirin (300 μM) were used as positive controls, and treated with IDX medium. After 14 days from the start of adipocyte differentiation of mesenchymal stem cells, the cell culture medium was removed, and the cells were washed with 10 ml of phosphate buffered saline (PBS). Then, 10 ml of formalin / PBS solution 0.2 ml / cm 2 2 was used to fix the cells for 10 minutes. The fixed cells were washed once with a 60% isopropanol solution (0.5 ml / cm 2 ), and the lipid in the adipocyte was stained with 0.2 ml / cm 2 of a dye solution obtained by dissolving Oil Red O in 60% isopropanol Respectively. Subsequently, re-staining was performed with hematoxylin to make it easy to distinguish the nucleus, and then observed with a microscope. The observation result is shown in Fig. As can be seen in FIG. 1, the extract of the wheat sprouts promoted differentiation of adipocytes without inducing apoptosis of mesenchymal stem cells.
실시예Example 3. 솜나물 추출물에 의한 인간의 3. Human sprout extract 중간엽줄기세포의Of mesenchymal stem cells 지방세포 분화 촉진 효과의 정량적 측정 Quantitative measurement of promoting effect of adipocyte differentiation
상기 실시예 2에서와 같이 중간엽줄기세포로부터 지방세포의 분화를 유도한 후 마지막 배지 교체를 한 후 이틀 뒤 세포배양 상층액에서 아디포넥틴의 발현양을 측정하여, 지방분화 촉진 효과에 대한 정량적 비교를 실시하였다. 아디포넥틴(adiponectin)은 지방세포에서 분비되는 대표적인 아디포카인(adipokine) 중 하나로서, 항당뇨작용을 비롯하여 다양한 효능을 나타내는 것으로 보고되어 있다. 예를 들어 당뇨병에 있어서 아디포넥틴은 인슐린 감수성을 증가시켜 혈당을 낮춤으로써 당뇨병을 예방하는 효능을 나타낸다. 이러한 아디포넥틴은 지방세포가 분화되면서 발현이 증가하는 단백질로 알려져 있다. 아디포넥틴과 다른 사이토카인의 측정은 알엔디시스템사(R&D Systems, Minneapolis, MN, USA)의 DuoSet Kit를 제조자의 방법대로 사용하였다. 결과로 도 2에서 볼 수 있는 바와 같이, IDX 에 의해 지방세포로의 분화를 유도한 경우, 솜나물 추출물이 처리되지 않은 것에 비해 중간엽줄기세포의 지방세포 분화가 솜나물 추출물이 처리된 실험군에서 아디포넥틴의 발현양이 통계적으로 유의성 있게 증가하는 것으로 나타났다. 통계는 일원분산분석(ANOVA) 후, post hoc test를 수행하여 검증하였으며, p 값이 0.05 이하일 때 유의적인 차이가 있는 것으로 표시하였다. After the induction of differentiation of adipocytes from the mesenchymal stem cells as in Example 2, and after the last medium change, the expression level of adiponectin was measured in the cell culture supernatant two days later, and quantitative comparison Respectively. Adiponectin is one of the representative adipokines secreted by adipocytes and has been reported to exhibit various effects including antidiabetic action. For example, in diabetes, adiponectin has the effect of preventing diabetes by lowering blood glucose by increasing insulin sensitivity. Such adiponectin is known to be a protein whose expression increases with the differentiation of adipocytes. Adiponectin and other cytokines were measured using DuoSet Kit from R & D Systems, Minneapolis, MN, USA according to the manufacturer's instructions. As a result, as shown in FIG. 2, when the adipocyte differentiation was induced by IDX, adipocyte differentiation of mesenchymal stem cells was observed in the expression group of adiponectin And the amount of this increase was statistically significant. Statistical analysis was performed by one-way analysis (ANOVA) followed by post hoc test. Significant differences were noted when the p-value was less than 0.05.
실시예Example 4. 솜나물 추출물에 의한 인간의 섬유아세포에서 콜라겐 합성 촉진 효과 4. Promotion of Collagen Synthesis in Human Fibroblast by the Extract of Sweetpotato
사람의 섬유아세포(human dermal fibroblast, HDFs)(Lonza, Inc. Walkersville, MD, USA)는 제조사의 방법대로 배양하였다. 콜라겐 타입1(BD Biosciences, Franklin Lakes, NJ, USA)과 상기 섬유아세포를 이용해 인공진피모델(human dermal equivalents, HDEs)을 만들었다. 구체적으로 인공진피의 콜라겐 농도는 1mg/ml 로 만들었으며 콜라겐과 섬유아세포 혼합물을 37.8°C 에서 60분 동안 중합을 진행시켰다. 3차원 콜라겐 겔(3-dimensional collagen gel)이 수축을 시작하기 전인 2~3일 이내에 실험을 진행했다. 인공진피모델에서 콜라겐 타입1 합성을 측정하기 위해 상층액을 취하여 프로콜라겐 타입1 C-펩타이드(Procollagen type I C-peptide, PICP)를 PICP ELISA kit (Takara Bio, Shiga, Japan)를 이용해서 정량적으로 확인했다. 결과로 도 3에서 볼 수 있는 바와 같이, 대조군에 비해 IDX를 처리한 경우에 HDEs에서 콜라겐 합성이 소폭 증가했고, 솜나물 추출물이 처리되지 않은 것에 비해 솜나물 추출물이 처리된 실험군에서 통계적으로 유의성 있게 콜라겐 합성이 증가함을 발견하였다. 통계는 일원분산분석(ANOVA) 후, post hoc test를 수행하여 검증하였으며, p 값이 0.05 이하일 때 유의적인 차이가 있다고 표시하였다. Human dermal fibroblasts (HDFs) (Lonza, Inc. Walkersville, MD, USA) were cultured according to the manufacturer's instructions. Collagen type 1 (BD Biosciences, Franklin Lakes, NJ, USA) and the fibroblasts to produce human dermal equivalents (HDEs). Specifically, the collagen concentration of artificial dermis was 1 mg / ml, and the collagen and fibroblast mixture was polymerized at 37.8 ° C for 60 minutes. Experiments were conducted within two to three days before 3-dimensional collagen gel started to contract. To determine collagen type 1 synthesis in the artificial dermis model, the supernatant was taken and quantified by using a PICP ELISA kit (Takara Bio, Shiga, Japan) using Procollagen type 1 C-peptide Confirmed. As a result, as shown in FIG. 3, collagen synthesis in HDEs was slightly increased in the case of IDX treatment compared to the control group, and in the experimental group treated with the somnut extract, the collagen synthesis , Respectively. Statistical analysis was performed by one-way ANOVA followed by post hoc test. Significant differences were noted when the p-value was less than 0.05.
이상에서 본원의 예시적인 실시예에 대하여 상세하게 설명하였지만 본원의 권리범위는 이에 한정되는 것은 아니고 다음의 청구범위에서 정의하고 있는 본원의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본원의 권리범위에 속하는 것이다.While the present invention has been described in connection with what is presently considered to be the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, .
본 발명에서 사용되는 모든 기술용어는, 달리 정의되지 않는 이상, 본 발명의 관련 분야에서 통상의 당업자가 일반적으로 이해하는 바와 같은 의미로 사용된다. 본 명세서에 참고문헌으로 기재되는 모든 간행물의 내용은 본 발명에 도입된다. All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. The contents of all publications referred to herein are incorporated herein by reference.
Claims (9)
A cosmetic composition for suppressing hypothecia reduction of subcutaneous fat tissue containing a spiny moss extract.
The cosmetic composition according to claim 1, wherein the extract is an organic solvent extract.
A cosmetic composition for accelerating collagen production, comprising a pine needle extract.
Wherein the skin care is wrinkle improvement, or an increase or improvement of skin elasticity, wherein the skin care cosmetic composition is a skin care cosmetic composition.
A cosmetic composition for promoting the differentiation of mesenchymal stem cells into subcutaneous adipose tissue, which comprises a somnuta extract, into adipocytes.
A cosmetic composition for improving skin wrinkles, comprising a wormwood extract.
A food composition for accelerating collagen production, comprising a wheat germ extract.
A kit for promoting the differentiation of mesenchymal stem cells into adipocytes by subcutaneous adipose tissue containing a spiny plant extract.
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