KR102214505B1 - Skin External Preparation and Cosmetic Compositions Comprising Extract of Viburnum erosum Thunb. - Google Patents

Abstract

The present invention relates to a cosmetic composition for antibacterial, anti-inflammatory, antioxidant and anti-wrinkle properties containing an extract of the viburnum erosum thunb as an active ingredient. Since the extract provided from the present invention has excellent antibacterial activity against acne-causing skin flora, inflammatory cytokine production inhibitory activity, DPPH scavenging activity, and elastase inhibitory activity, the extract can be used as the cosmetic composition for antibacterial, anti-inflammatory, antioxidant and anti-wrinkle properties.

Description

Skin External Preparation and Cosmetic Compositions Comprising Extract of Viburnum erosum Thunb.}

The present invention relates to an external preparation for skin and a cosmetic composition comprising an extract of Viburnum erosum Thunb.

Human skin is an organ that directly contacts the surrounding environment, and infections such as bacteria and fungi that are involved in various skin diseases easily occur. For example, Stein Staphylococcus aureus causing maturation skin Philo nose Syracuse aureus (Staphylococcus aureus) for Philo, including skin flora Stein nose Syracuse epidermidis (Staphlyococcus epidermidis), acne bacteria is Propionibacterium sludge tumefaciens arc Ness (Propionibacterium acnes ) exists in the skin, and these bacteria decompose sebum or sweat secreted on the skin to generate odor, and the decomposition products irritate the skin and cause inflammation. In particular, Propionibacterium acnes uses a fatty acid degrading enzyme, lipase, to decompose sebum to produce free fatty acids. These free fatty acids not only irritate the skin, but also cause inflammatory lesions such as papules, pustules, and nodules, which are red rashes found in acne.

Antimicrobial compounds such as triclocarban and triclosan have been used as antibacterial substances against these bacteria, but they are known to cause skin side effects such as skin burning, burning, and redness when used for a long time.

As described above, the conventional antimicrobial synthetic material and cosmetic composition including the same may cause skin irritation, and skin troubles may occur. Accordingly, a new antimicrobial material is required.

Inflammation refers to the pathological condition of an abscess formed by the invasion of external infectious agents (bacteria, fungi, viruses, and various allergens). In other words, inflammation is one of the immune reactions in the body, and it works when external factors invade into the body. In the case of irritation or scarring on the skin, or when looking at the periphery of purulent acne, swelling and redness can be observed. Was created. Specifically, when external bacteria invade a specific tissue and proliferate, the white blood cells in the living body recognize this and actively attack the expanded external bacteria, and the dead damage of the white blood cells generated during this process accumulates in the tissues invaded by the bacteria. At the same time, the cell destruction of the invading bacteria killed by white blood cells melts into the invaded tissue, forming an abscess. The treatment of abscesses caused by inflammation can be promoted through anti-inflammatory action.Anti-inflammatory action is to inhibit the proliferation of invading bacteria by using antibacterial agents or to digest foreign substances by activating macrophages that eat foreign substances accumulated in the abscess. It is an effect of promoting inflammation treatment, such as enhancing the function of excreted macrophages.

In general, the inflammatory reaction is a biodefensive reaction process for repairing and regenerating damage caused by invasion that causes organic changes in cells or tissues of a living body, and this reaction process includes local blood vessels, various tissue cells of body fluid, and immune cells. This works. While the inflammatory response normally induced by external invading bacteria is a defense system for protecting the living body, when abnormally excessive inflammatory response is induced, various diseases appear, and these diseases are called inflammatory diseases. Inflammatory disease is a disease that threatens the life of the human body by amplifying and sustaining inflammation by various inflammatory mediators secreted from target cells activated by external stimulation. In the form of diseases in the joints such as acute inflammation, rheumatoid arthritis, psoriasis, etc. It includes allergic inflammatory diseases such as skin diseases and bronchial asthma.

Reactive oxygen introduced from outside the body or generated in the body promotes aging in the body or may cause cancer. Therefore, research on antioxidants that inhibit oxidation by free radicals are being conducted, and antioxidants are known as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium. However, when applied to the skin, antioxidants that exist in nature cannot be expected to have a substantial effect. On the other hand, synthetic antioxidants having excellent antioxidant power and low cost have a problem that their use is limited due to concerns about safety such as side effects on the human body.

The causes of wrinkle formation can be largely divided into wrinkle formation due to natural aging and wrinkle formation due to UV exposure (photoaging), and the mechanism of wrinkle formation due to the two causes has not yet been accurately identified. Common phenomena found in relation to skin changes due to natural aging and photoaging are the reduction and transformation of collagen and elastin, the main constituent proteins in the dermis, and reduction of skin elasticity due to the reduction of hyaluronic acid, the matrix of the dermis. In particular, according to the results of the studies so far, it is known that the reduction and transformation of collagen and elastin, the main proteins in the dermis, are directly involved in the formation of wrinkles and the reduction of elasticity of the skin. Collagen is a major matrix protein produced by fibroblasts of the skin and is present in the extracellular interstitial. Its important functions include skin mechanical firmness, connective tissue resistance and tissue bonding, support for cell adhesion, cell division and differentiation (organic Growth or induction of wound healing) is known. Such collagen is reduced by age and photoaging caused by ultraviolet irradiation, which is known to be closely related to the formation of wrinkles in the skin. In addition, as the results of extensive research on skin aging have recently been reported, an important function of collagen in the skin has been revealed. Active ingredients that promote collagen synthesis to improve wrinkles are known. For example, retinoic acid, TGF (transforming growth factor), animal placenta-derived proteins, betulinic acid, chlorella extract, and the like are known as collagen synthesis promoting substances.

On the other hand, Viburnum erosum Thunb. is a tree commonly found in low mountain areas in the south of Gyeonggi-do. It is a small tree of the Indong family, and its height is 2~3m. The white flowers in spring and red fruits in autumn are beautiful and are planted as park trees. The oval leaves run opposite to each other. The fur on the front and back side gives a soft feeling to the touch. The stem is split into several pieces and grows to form an abandonment. Flowers bloom from April to May with white bisexual flowers forming a round shape at the end of a new branch. Small fruits that look like red beans ripen red in September-October and are good food for birds. It grows in moderately sunny forests and likes soil with good moisture retention and drainage. It has very strong cold resistance to withstand cold and grows well in both sunny and dark areas. Withstands well in dry environments.

The efficacy of the pheasant tree is used to treat numbness and pain in joints and muscles, itching of the skin, inflammation of the mouth, and eczema. It is also effective against spots and freckles. The fruit of the pheasant tree eliminates blood stagnation, relieves swelling, and stops dysentery.

As a cosmetic composition technology using the extract of the pheasant tree, in the case of Korea, Korean Patent Publication No. 10-1630801 "Anti-inflammatory composition" is disclosed.

However, there has not yet been a study on the development of a cosmetic composition having antibacterial, anti-inflammatory, antioxidant and wrinkle-improving effects by using the extract of the pheasant tree.

Accordingly, the inventors of the present invention show that the extract of Viburnum erosum Thunb. has excellent antibacterial activity against acne-causing skin fungi, inhibiting inflammatory cytokine production, DPPH scavenging activity, and elastase inhibitory activity, antibacterial, The present invention was completed by discovering that anti-inflammatory, antioxidant, and wrinkle-improving effects can be provided at the same time.

Korean Patent Publication No. 10-1630801

An object of the present invention is to provide a cosmetic composition for antibacterial, anti-inflammatory, antioxidant and wrinkle improvement derived from natural products.

Another object of the present invention is antibacterial, anti-inflammatory, antioxidant and wrinkle improvement derived from natural products It is to provide a cosmetic comprising a cosmetic composition.

In order to achieve the above object, the present invention provides a cosmetic composition for antibacterial, anti-inflammatory, anti-oxidation and wrinkle improvement, comprising the extract of dulcis pheasant tree ( Viburnum erosum Thunb.) as an active ingredient.

In the present invention, the extract is characterized in that it is extracted with a solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, glycerin, and a mixed solvent thereof.

In the present invention, the extract is characterized in that it contains 0.001 to 20% by weight based on the total weight of the composition.

In the present invention, the extract is characterized in that it has an antimicrobial activity against acne-causing dermatophytes, inhibiting inflammatory cytokine production, scavenging DPPH, and inhibiting elastase.

In addition, the present invention provides a cosmetic comprising the composition.

The extract provided from the present invention has excellent antimicrobial activity against acne-causing dermatophytes, inflammatory cytokine production inhibitory activity, DPPH scavenging activity, and elastase inhibitory activity, so antibacterial, anti-inflammatory, antioxidant and wrinkle It can be used as an improvement cosmetic composition.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.

Throughout the specification of the present application, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

According to one embodiment of the present invention, the present invention relates to a cosmetic composition for antibacterial, anti-inflammatory, antioxidant, and wrinkle improvement comprising an extract of Viburnum erosum Thunb.

The extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention belongs, and specifically, from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and a mixed solvent thereof It may be extracted with a selected solvent.

Specifically, the content of the extraction solvent may be 2 to 100 times, more preferably 5 to 50 times, most preferably 10 to 20 times the weight of the plant solids.

In addition, the extract may be extracted by heating or at room temperature under conditions in which the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and ultrasonic extraction, cold needle extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction, and the like may be used depending on the type and extraction method of the extract.

Filtration is a process of removing suspended solid particles from the extract. The particles may be filtered using cotton, nylon, paper, or the like, or ultrafiltration, freezing filtration, centrifugation, etc. may be used, but is not limited thereto.

The step of concentrating the filtrate and then drying it includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.

According to one embodiment of the present invention, the extract may be processed and used in various forms, such as a fermentation broth, a powder obtained by drying the extract, and a concentrate obtained by concentrating and filtering the extract.

According to one embodiment of the invention, the deolkkwong tree (Viburnum erosum Thunb.) Extract may contain from 0.001 to 20% by weight relative to the total composition weight, and more preferably contain from 0.1 to 1% by weight, obtained from the same method as described above I can. When the extract is less than 0.001% by weight based on the total weight of the composition, the function as a composition is poor because it cannot sufficiently obtain antibacterial, anti-inflammatory, antioxidant, and wrinkle improvement effects, and when it exceeds 20% by weight, it does not contain more Not only is it uneconomical because it can exhibit sufficient efficacy without it, but also it is not preferable because it is difficult to use as a cosmetic due to deterioration of the stability and spreadability of the formulation.

The extract is characterized in that it has antibacterial activity against acne-causing dermatophytes, inhibitory activity of inflammatory cytokine production, DPPH scavenging activity, and elastase inhibitory activity. That is, because it can provide antibacterial, anti-inflammatory, antioxidant, and wrinkle improvement effects at the same time, not only skin beauty but also fundamental skin health improvement can be realized.

According to one embodiment of the present invention, the inflammatory cytokine is a substance involved in inflammation accompanying tissue damage, bacterial and viral infections, tumors, etc., and is produced from lymphocytes or macrophages. Target inflammatory cytokines include, for example, interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor (TNF-α). In the present invention, interleukin-6 (IL-6) and interleukin-8 (IL-8) are particularly suitable targets.

As described above, the extract has excellent antibacterial activity against acne-causing skin flora, inflammatory cytokine production inhibitory activity, DPPH scavenging activity, and elastase inhibitory activity, so antibacterial, anti-inflammatory, antioxidant and wrinkle It can be used as an improvement composition.

According to one embodiment of the present invention, when used as the cosmetic composition, skin ointment, cream, soft lotion, astringent lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, spray , Gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion, and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, titanium oxide, etc. may be used as carrier components. I can.

When the formulation of the present invention is a body gel, in addition to the above-described active ingredients, components generally used in the manufacture of body gels, such as carbomer, triethanolamine, menthol, ethanol, glycerin, disodium EDTA, methylparaben, fragrance, etc. Can be used. Here, carbomer and triethanolamine act as a thickening agent, menthol acts as a fragrance ingredient, ethanol acts as a refreshing sensation, glycerin acts as a moisturizer, and disodium EDTA is a metal chelate. It functions as, and methylparaben is added to function as a preservative. In addition, in addition to menthol flavor, various flavors such as herbal flavor may be added to the fragrance.

In addition to the above-described specific ingredients, it is also within the scope of the present invention to apply known substances widely used in the art to achieve the functions of thickeners, cool sensations, fragrance ingredients, and moisturizers described above. Can be included.

When the formulation of the present invention is a powder or spray (aerosol), lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be added as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane/butane or dimethyl ether may be included.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is added as a carrier component, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

According to one embodiment of the present invention, the cosmetic composition may be prepared as a composition such as body gel, body lotion, body cream, body oil, etc. for promoting fat decomposition by adding conventional additives, and may also be prepared in an aerosol type. I can. In this case, the cosmetic composition is preferably used as a method of transdermal administration by directly applying or spraying on the skin.

According to one embodiment of the present invention, the amount of use of the cosmetic composition may be appropriately adjusted according to individual differences such as age and skin fat condition, formulation, and shape, and for antibacterial, anti-inflammatory, antioxidant, and wrinkle improvement effects. It can be usefully used as a composition.

The cosmetic composition obtained from the above method exhibits antibacterial activity against acne-causing dermatophytes, inhibiting inflammatory cytokine production, DPPH scavenging activity, and elastase inhibitory activity, and thus excellent antibacterial, anti-inflammatory, antioxidant and wrinkle Cosmetics for improvement can be provided.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and that the scope of the present invention is not limited by these examples according to the gist of the present invention, for those of ordinary skill in the art to which the present invention belongs. It will be self-evident.

<Example 1> Preparation of dulcis pheasant tree extract

1 L of 75% (v/v) ethanol aqueous solution was added to 100 g of a pheasant tree (collection), followed by warm extraction at 60° C. for 3 hours. After extraction, filtration through a filter paper, repeating the extraction process twice, and then concentrating under reduced pressure to prepare a final powdered red pheasant extract.

<Experimental Example 1> Antibacterial efficacy test: Paper Disc Method

In order to investigate the antibacterial effect using the pheasant tree extract of Example 1, Propionibacterium acnes (KCTC 3314, Biological Resource Center), Staphylococcus epidermidis (KCCM 35494, Korea) Microbial Conservation Center), Staphylococcus aureus (ATCC 25923, American Type Culture Collection, USA) antibacterial activity was measured.

Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus, respectively, on a solid agar medium coated with the extract of Example 1 (5%, 50 μL) and control (DMSO, 50 μL) Each of the disks buried with was placed on it and cultured, and the antibacterial activity was measured. The antibacterial activity was measured using the Paper Disc Method, and the size of the transparent ring around the disk indicates the antibacterial activity.

<Antibacterial evaluation>

-: Cannot create invisibility ring

+: Creates a weak invisibility ring

++: Create strong invisibility ring

+++: Create a very strong invisibility ring

Propionibacterium acnes Staphylococcus epithemedis Staphylococcus aureus Control - - - Example ++ ++ ++

As a result, as can be seen in Table 1, it was confirmed that the antibacterial activity against acne-causing dermatophytes was superior to the dulcis pheasant extract of Example 1 compared to the control group.

<Experimental Example 2> Anti-inflammatory efficacy test: inflammatory cytokine production inhibitory activity

In order to investigate the effect of alleviating inflammation caused by Propionibacterium acnes (KCTC 3314, Biological Resource Center) using the extract of the pheasant tree of Example 1, keratinocytes (HaCaT) using acne bacteria cells), the degree of inhibition of inflammation-related cytokine production was measured.

Human-derived keratinocytes (HaCaT cells) were pre-sale from ATCC (American Type Culture Collection, USA), and DMEM medium containing 10% FBS (fetal bovine serum, Gibco BRL, USA) (Dulbecco's Modified Eagle's Medium, HyClone Lab. , USA) was cultured under 37°C, 5% CO ₂ .

The cultured HaCaT cells were dispensed into a 24-well plate at 1×10 ⁵ cells/mL per well, and cultured for 24 hours. After cultivation, the extract was replaced with a DMEM medium that did not contain syrum diluted by concentration, treated for 30 minutes, treated with acne bacteria killed by heat treatment, and cultured for 24 hours. The amount of inflammation-related cytokines IL-8 and IL-6 was measured using an ELISA assay kit (BD OpTEIA Human IL-8 ELISA Kit, BD OpTEIA Human IL-6 ELISA Kit, BD Biosciences, USA). The absorbance of the cell culture solution that was not treated with the sample was set to 100% of the control group, and the experiment was performed three times each and expressed as the mean value ± standard deviation.

Propionibacterium acnes Concentration (μg/mL) IL-8 amount (%) IL-6 amount (%) - 0 32.7 ± 1.6 26.2 ± 4.9 + 0 100 100 One 101.2 ± 12.8 111.4 ± 17.4 5 70.4 ± 9.2 96.2 ± 6.0 10 53.3 ± 4.5 88.7 ± 6.9 20 38.7 ± 2.6 62.9 ± 4.0

As a result, as can be seen in Table 2, the extract of dulcis pheasant in Example 1 showed an excellent anti-inflammatory effect by reducing IL-8 and IL-6 induced by Propionibacterium acnes in a concentration-dependent manner.

<Experimental Example 3> Antioxidant test: DPPH scavenging activity

The free radical scavenging activity was evaluated in order to investigate the antioxidant effect using the extract of the pheasant tree of Example 1.

The free radical scavenging activity experiment was modified by Kim et al. (Kor. J. Pharmacogn., 24(4), 299~303, 1993), and is a stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl). , Sigma) reagent was used. First, 1 mL of 0.2 mM DPPH solution was diluted with 2 mL of dulcis pheasant extract of Example 1 in methanol and mixed with 2 mL, reacted at room temperature for 10 minutes, and absorbance was measured at 517 nm with a microplate reader (Synergy, BioTek, USA). . On the other hand, the control group in the free radical scavenging test was measured by adding methanol instead of the sample solution, and methanol was added instead of the DPPH solution to obtain a color correction value for each. The experiment was performed three times each and expressed as the mean value ± standard deviation, and the free radical elimination rate was calculated using Equation 1 below.

[Equation 1]

Free radical scavenging rate (%) = 100-(absorbance of each extract sample/absorbance of control) x 100

Concentration (μg/mL) Erasure rate (%) 15.625 19.1 ± 1.1 31.2 34.8 ± 0.9 62.5 54.1 ± 0.9 125 79.1 ± 0.3

As a result, as can be seen in Table 3, the extract of Deulpiangi tree of Example 1 showed an excellent free radical scavenging rate in a concentration-dependent manner, and it was confirmed that it has antioxidant efficacy.

<Experimental Example 4> Wrinkle improvement efficacy test: Elastase inhibition activity measurement

The Elastase Inhibition Activity Test was performed in order to examine the effect of improving and inhibiting skin wrinkles using the extract of dulcis pheasant of Example 1.

This experiment is a method of measuring the activity of elastase, an enzyme that decomposes elastin. The color generated when ρ-nitroaniline is decomposed using N-Succinyl-(Ala)3-ρ-nitroaniline, an elastase substrate. This is a method of measuring the elastase activity by measuring the absorbance at a wavelength of 405 nm. The buffer solution is pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution is 8.8 mM N-Succinyl-(Ala)3-ρ-nitroaniline (Sigma), and the enzyme solution is porcine pancreatic elastase at 10 μg/mL (Sigma). Used in concentration. After mixing 60 μL of buffer solution, 20 μL of substrate solution, and the sample solution of 100 μL by diluting the dulcis pheasant extract of Example 1 in purified water by concentration, add 20 μL of enzyme solution and react in a constant temperature water bath at 25°C for 15 minutes to determine the amount of ρ-nitroaniline produced It was measured at a wavelength of 405 nm using a reader (Synergy, BioTek, USA). At this time, the control for measuring the elastase activity was measured in the same way by putting purified water instead of the sample solution, and purified water was added instead of the enzyme solution to obtain a color correction value for each. Each experiment was performed three times and expressed as the mean value ± standard deviation, and the elastase activity was calculated using Equation 2 below.

[Equation 2]

Elastase activity inhibition rate (%) = 100-(absorbance of each extract sample/absorbance of control) x 100

Concentration (mg/mL) Inhibition rate (%) 0.1 31.9 ± 6.5 0.15 42.5 ± 6.8 0.2 66.5 ± 1.6 0.3 75.5 ± 3.7

As a result, as can be seen in Table 4, the extract of dulcis pheasant in Example 1 showed excellent elastatase inhibitory activity in a concentration-dependent manner, and it was confirmed that it has an effect of improving wrinkles.

As described above, specific parts of the present invention have been described in detail, and it will be apparent to those of ordinary skill in the art that these specific techniques are only preferred embodiments, and the scope of the invention is not limited thereby will be. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (5)

A cosmetic composition having antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis , comprising an ethanol extract of Viburnum erosum Thunb.


delete The method of claim 1, wherein the extract contains 0.001 to 20% by weight based on the total weight of the composition. Propionibacterium acnes comprising the extract of Viburnum erosum Thunb. And Staphylococcus epidermidis ( Staphylococcus epidermidis ) Cosmetic composition having antibacterial activity.



delete A cosmetic product comprising the composition of any one of claims 1 and 3.