KR20210001260A - Cosmetic compositions having Antioxidant effect comprising Phloretin, Fragaria Vesca (Strawberry) Leaf Extract and Leontopodium Alpinum Callus Culture Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antioxidant effect. The present invention provides a cosmetic composition having anti-inflammatory, anti-allergic and anti-atopic effects along with antioxidant efficacy, wherein the cosmetic composition contains a combination of a Fragaria vesca leaf extract, phloretin, and an edelweiss callus culture extract as active ingredients to prevent skin oxidation, thereby preventing aging and improving skin transparency.

Description

Cosmetic compositions having Antioxidant effect comprising Phloretin, Fragaria Vesca (Strawberry) Leaf Extract and Leontopodium Alpinum Callus Culture Extract, containing wild strawberry leaf extract, phloretin and edelweiss callus culture extract.

The present invention relates to a cosmetic composition having an antioxidant effect, and more specifically, a cosmetic composition having excellent skin penetration and antioxidant, anti-inflammatory, anti-allergic and anti-atopic efficacy by containing wild strawberry leaf extract, phloretin, and edelweiss callus culture extract. It relates to the composition.

Skin aging can be largely divided into two types: intrinsic (chronological aging) and photoaging (phtoaging). Endogenous aging is a phenomenon that occurs due to a decrease in physiological functions of a living body as age increases, and is a naturally occurring aging phenomenon. In contrast, photoaging means that the appearance or function of the skin changes as the skin is repeatedly exposed to light.

In addition, skin aging can activate reactive oxygen species due to factors such as ultraviolet rays, stress, disease state, and environmental factors, and if such a condition is deepened, it destroys the antioxidant defense network existing in the living body and damages cells and tissues, leading to adult diseases and It accelerates aging.

Specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major components of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.In particular, protein oxidation is caused by collagen, which is the connective tissue of the skin. Hyaluronic acid, elastin, proteoglycan, fibronectin, etc. are cut, causing a severe excessive inflammatory reaction, which interferes with the elasticity of the skin. Further, when the symptoms become severe, mutations in DNA occur, which leads to mutations, cancer, and immune function decline.

The theory that receives the most support for aging in skin tissue is the free radical theory. According to this theory, free radicals are generated in the living body by metabolic reactions, ultraviolet rays, smoke and environmental hormones, and the antioxidant barriers are destroyed by these free radicals, thereby oxidizing lipids, proteins, polysaccharides, and nucleic acids, which are major components of the skin. As a result, it is said that it accelerates the aging process of skin cells and tissues. In other words, the skin is subjected to oxidative stress by radicals such as superoxide radicals (O2-) and hydroxy radicals (OH) and non-radicals such as hydrogen peroxide (H2O2), thereby damaging cells and promoting skin aging. . In particular, when the protein is oxidized, collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, etc., which are the skin's connective tissues, are cleaved, leading to severe excessive inflammatory reactions and decreased skin elasticity. When this becomes worse, mutations in the DNA lead to mutations, cancer, and immune function decline.

Therefore, in order to prevent skin aging, it is necessary to prevent skin damage by scavenging free radicals generated by various causes, and already damaged cells need to be recovered by regeneration and proliferation through active metabolism. Accordingly, interest and research on the development of cosmetics with functions such as antioxidant, whitening, anti-inflammatory, anti-allergic and anti-atopy using natural extracts are being conducted steadily.

Accordingly, the present inventors have repeatedly conducted studies to prevent skin oxidation to prevent aging and improve skin transparency, and as a result, a combination of wild strawberry leaf extract, phloretin, and Edelweiss callus culture extract (plant cell culture extract derived from Edelweiss) is effective. When contained as a component, it was confirmed that it has far superior antioxidant, anti-inflammatory, anti-allergic and anti-atopic efficacy than when these components are used alone.

Republic of Korea Patent Publication No. 10-2015-0066805

The present invention is to solve the above problems, a cosmetic composition having an antioxidant effect that prevents aging and improves skin transparency by containing a complex of wild strawberry leaf extract, phloretin, and edelweiss callus culture extract as active ingredients Is to provide.

In addition, the present invention is to provide a cosmetic composition having anti-inflammatory, anti-allergic and anti-atopic effects by containing a complex of wild strawberry leaf extract, phloretin, and edelweiss callus culture extract as active ingredients.

However, the objects of the present invention are not limited to the above-mentioned objects, and other objects that are not mentioned will be clearly understood by those skilled in the art from the following description.

To achieve the above object,

Cosmetic composition having an antioxidant effect according to the present invention,

It characterized in that it contains wild strawberry leaf extract, floretin, and culture extract of Edelweiss callus.

In addition, the cosmetic composition having an antioxidant effect according to the present invention,

Characterized in that it contains a complex of wild strawberry leaf extract, phloretin, and culture extract of Edelweiss callus in an amount of 0.01 to 10% by weight, preferably 0.1 to 5% by weight, more preferably 1 to 3% by weight, based on the total weight of the cosmetic composition. do.

If the content of the composite is less than 0.01% by weight, there is a problem of limiting effective performance, and if it exceeds 10% by weight, there is a problem in formulation and formulation stabilization.

In addition, the cosmetic composition having an antioxidant effect according to the present invention,

The mixing ratio (% by weight) of the wild strawberry leaf extract, the phloretin, and the Edelweiss callus culture extract to form a complex is a wild strawberry leaf extract: Phloretin: Edelweiss callus culture extract = 1:1 (% by weight) or 1.5 It is characterized in that the ratio is 1:1.5 (% by weight).

In addition, the cosmetic composition having an antioxidant effect according to the present invention,

Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion , Cleansing cream, body lotion and body cleanser is characterized in that it is implemented in one or more formulations selected from the group consisting of formulations.

In addition, the cosmetic composition according to the present invention is characterized in that it has anti-inflammatory, anti-allergic and anti-atopic effects containing wild strawberry leaf extract, phloretin, and edelweiss callus culture extract as active ingredients.

The cosmetic composition having an antioxidant effect according to the present invention contains a combination of wild strawberry leaf extract, phloretin, and edelweiss callus culture extract as active ingredients, preventing skin oxidation, preventing aging and improving skin transparency, along with anti-inflammatory properties, It has anti-allergic and anti-atopic efficacy.

Hereinafter, a preferred embodiment of the present invention will be described. In the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

The present invention contains a complex of wild strawberry leaf extract, floretin, and edelweiss callus culture extract as active ingredients to prevent skin oxidation, thereby preventing aging and improving skin clarity. Cosmetics having antioxidant, anti-inflammatory, anti-allergic, and anti-atopic effects The composition is provided.

The wild strawberry leaves of the present specification are rich in polyphenols and thus have excellent antioxidant effects, and at the same time, they improve skin diseases such as allergy and atopy, and help maintain healthy skin by strengthening skin defenses with anti-inflammatory effects.

The wild strawberry leaf extract according to the present invention can be prepared through ultra-high pressure ethanol extraction. That is, the ultra-high pressure ethanol extraction process conditions of the present invention are 100 Mpa to 1,000 Mpa, preferably 500 Mpa, for 5 minutes to 13 minutes, preferably by immersing wild strawberry leaves in 70% ethanol and then using an ultra-high pressure extraction device at room temperature. It is extracted by adding it for 9 minutes.

Ultra-high pressure extraction is a method of accelerating the elution of components by breaking the cell wall and tissue and increasing the efficiency by obtaining a high elution amount in a short time.By applying high pressure to shorten the extraction time, it reduces denaturation by heating and toxicity due to solvents. . Therefore, when the pressing force and the pressing time are smaller than the minimum range, the effective ingredient elution efficiency is small, and when it is larger than the maximum range, the active ingredient is denatured and the desired effect cannot be obtained.

In addition, when using ultra-high pressure extraction, components such as flavonoids and triterpenoids, in addition to polyphenols having antioxidant activity, are eluted from the wild strawberry leaf extract of the present invention, in addition to the flavor, color, and essential oil components of wild strawberry leaves. The elution of the back becomes possible.

The wild strawberry leaf extract contained in the composite of the present invention is contained in an amount of 1 to 90% by weight, preferably 10 to 50% by weight, and more preferably 30 to 40% by weight, based on the total composite. When the content of the wild strawberry leaf extract is less than 1% by weight, the antioxidant effect and skin defense strengthening effect of the wild strawberry leaf extract is weak, and when it exceeds 90% by weight, the increase in the effect due to the increase in the content is very weak, and the relative As the content of other ingredients is small, there is a problem that the synergistic effect by the composite is insignificant.

Phloretin is a phenol derived from a dihydrochalcone plant having a C6-C3-C6 skeleton, which is abundant in apples (mainly peel) and apple leaves, and is free or glucosidic form of phloridzin, (phloretin 2'-O-glucose)). Apple peel contains 0.2~5mg/g of phloretin. Phloretin has many biological functions, including antioxidant and anticancer activity, and is associated with the prevention of heart disease. This ingredient is particularly superior to other antioxidants in its ability to cross the skin barrier.

As such, phloretin has a poorly soluble property that is not soluble in water and oil. By improving it, hydrophilicity is increased, and it is well soluble in water or oil, and at the same time, it is manufactured so as not to lose its activity. It goes without saying that a phloretin derivative can be used.

The phloretin contained in the composite of the present invention is contained in an amount of 1 to 90% by weight, preferably 10 to 50% by weight, more preferably 30 to 40% by weight, based on the total composite. When the content of floretin is less than 1% by weight, the UV protection effect and skin improvement effect of floretin is weak, and when it exceeds 90% by weight, the increase in the effect due to the increase in the content is very weak. Due to its low content, there is a problem that the synergistic effect of the composite is insignificant.

Edelweiss is a plant that lives in alpine areas such as the Alps, and because it grows naturally after being exposed to extreme cold and ultraviolet rays, it contains natural defense substances that protect itself from ultraviolet rays and free radicals, preventing skin oxidation to prevent aging and improve skin transparency. Helps to improve. In addition, Edelweiss contains a lot of flavonoids that are beneficial to skin health, such as luteolin, luteolin-7-glucoside, and leontopodic acid, so it has anti-inflammatory and antioxidant effects.Edelweiss extract is a natural extract and is not harmful to the human body. Gives excellent skin moisturizing effect.

The Edelweiss callus culture extract according to the present invention can be obtained by cutting all or part of an Edelweiss stem, flower, or leaf and culturing and extracting it in an induction medium.

Here, culture induction relates to plant tissue cultivation, and is to proliferate plant cells, organs, and plants by culturing living plant tissue aseptically in vitro. Since plant tissue cultivation uses the basic characteristics of plants, it is based on the ability to regenerate plants from somatic cells of plants, which is a unique feature of plants.

"Callus culture" refers to a plant cell culture, and is a special tissue or cell that occurs when a tissue cut from a plant is cultivated in a medium containing auxin, a kind of plant is wounded, or a supernatant is treated with auxin. Means lump. Callus is an amorphous tissue or cell mass that has lost its ability to cause normal organ formation or tissue differentiation, and is mostly composed of flow cells, and may also include plant tumor tissue.

The callus culture of the present invention may be derived from any part of Edelweiss stem, flower, leaf, etc., or derived by cutting cotyledons from germinated seedlings of Edelweiss seeds.

Edelweiss callus culture extract is prepared by putting the dried Edelweiss callus in purified water, first extraction while stirring at about 50° C. for about 8 hours, and hot water extraction at about 98° C. for about 10 minutes, or extracted using an ultrasonic extraction method. , May be a fermented extract.

Here, the dried Edelweiss callus can be obtained by culturing initial plant cells by germinating Edelweiss seeds, washing the completed plant cells three times with purified water, dehydrating, and drying in a freeze dryer.

The Edelweiss callus culture extract contained in the composite of the present invention is contained in an amount of 1 to 90% by weight, preferably 10 to 50% by weight, more preferably 30 to 40% by weight, based on the total complex. When the content of Edelweiss callus culture extract is less than 1% by weight, the antioxidant effect, anti-wrinkle effect and anti-inflammatory effect of Edelweiss callus culture extract is weak, and when it exceeds 90% by weight, the increase in the effect due to the increase in content is very slight And, there is a problem that the synergistic effect of the composite is insignificant because the content of other components is relatively small.

Example 1: Preparation of wild strawberry leaf extract

100 g of wild strawberry leaves are immersed in 70% ethanol solvent and ultra-high pressure ethanol extraction (HPEE) is performed by applying a pressure of approximately 500 MPa for 9 minutes at room temperature, and the extract is filtered through a filter, concentrated under reduced pressure at 30°C, and freeze-dried to solid content. Was obtained.

As a result of ultra-high-pressure ethanol extraction, the polyphenol content of the wild strawberry leaf extract was 337.81 mg/g dry, showing a high content, and the components such as flavonoids that give antioxidant activity and triterpenoids, which are an antioxidant activity and immune enhancing substance, are also high. Eluted.

Example 2: Preparation of phloretin

After adding 150mℓ of methanol to 100g of a sample of apple fruit, extracting it by grinding it with a mixer for 10 minutes, filtering it with a filter paper, concentrating the filtered methanol extract under reduced pressure, suspending the methanol extract in 50mℓ of water again, and adding the same amount of hexane to apples. It is added to the water suspension of the fruit extract, shaken several times, and left to equilibrate between the water layer and the hexane layer. Then, the hexane layer is taken and concentrated under reduced pressure, and the same amount of chloroform is added to the remaining water layer and shaken several times, and the layer is left to separate. When the water layer and the chloroform layer are divided, the chloroform layer is taken, concentrated under reduced pressure, and purified by silica gel column, recrystallization, or the like, to separate phloletin.

Example 3: Preparation of Edelweiss callus culture extract

100 g of dried Edelweiss callus powder obtained by washing, dehydrating and drying the edelweiss stem, flower or leaf, and plant cells cultured in germinated seedlings of Edelweiss seeds were added to 1 liter of purified water, followed by stirring at 50°C for 8 hours. After extraction, hot water extraction was performed at 98° C. for 10 minutes. After filtering the extract with a filter, it was concentrated under reduced pressure at 30° C. and freeze-dried to obtain a solid.

Example 4: Preparation of a complex of wild strawberry leaf extract, phloretin, and culture extract of Edelweiss callus

The wild strawberry leaf extracts of Examples 1 to 3, floretin, and the culture extract of Edelweiss callus wild strawberry leaf extract: floretin: Edelweiss callus culture extract = 1:1:1 (wt%) ratio or 1.5:1:1.5 ( A composite is prepared by mixing at a ratio of% by weight).

Experimental Example 1: Measurement of the antioxidant activity efficacy of the composite according to the present invention

In order to measure the antioxidant activity efficacy of the complex of wild strawberry leaf extract, phloretin, and Edelweiss callus culture extract according to the present invention, DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power by FRAP, and SOD-like activity were measured. I did. Here, VitaminC (0-1000 μg/mL) was used as a control.

1-1 DPPH radical scavenging activity efficacy measurement

DPPH radical scavenging activity (electron donating ability) was measured by modifying the method of Blois, and 3 ml of 60 μm DPPH was added to 0.5 ml of each sample, vortexed, and allowed to stand for 15 minutes, and the absorbance was checked at 517 nm.

As a result, the inhibitory effect of radical generation of the complex was measured to be a little greater than 1/2 of the value of Vitamin C at 50㎍/mL, and it was determined that it produced almost the same effect as Vitamin C at 100㎍/mL.

From this result, when compared with the control group, it was confirmed that in the concentration range of 50 to 100 μg/mL and in the range of 100 μg/mL or more and 500 μg/mL or less, the radical generation inhibitory effect of the complex was superior to that of the control group.

1-2 ABTS radical scavenging ability measurement

In addition, the ABTS radical scavenging ability was measured by mixing 7mM ABTS and 140mM K2S2O8 in 5ml:88µl and leaving it in a dark place for 14-16 hours, then mixing it with absolute ethanol at a ratio of 1:88 and absorbance value of the control group at 734nm. ABTS solution adjusted to be 0.7 was used. In addition, 50 µl of the composite sample solution and 1 ㎖ of ABTS solution were mixed for 30 seconds and incubated for 25 minutes, and the absorbance was measured at 734 nm to calculate the inhibition rate.

As a result, the radical scavenging ability of the complex was measured as 2/3 of the value of Vitamin C at 50 μg/mL, and 4/5 of the value of Vitamin C at 100 μg/mL. From this result, it was confirmed that the radical scavenging ability of the composite was excellent when compared with the control group.

1-3 Measurement of reducing power by FRAP

In addition, as a result of the experiment on the reducing power by FRAP, the reducing power by FRAP of the composite was 38.5 to 609 in the concentration range of 5 to 100 μg/mL.

That is, it was confirmed that the reducing power by FRAP of the composite was excellent in the low concentration range.

1-4 SOD-like activity measurement

In addition, in order to measure the SOD-like activity, 2.6 ml of Tris-HCl buffer (50mM tris+10mM EDTA, pH 85) and 0.2 ml of 7.2mM pyrogallol were added to 0.2 ml of each sample solution and reacted at 25°C for 10 minutes. After that, 0.1 ml of 1M HCl was added to stop the reaction, and the amount of oxidized pyrogallol in the reaction solution was measured at 420 nm. The SOD-like activity was expressed as the rate of decrease in absorbance between the added and non-added sample solutions.

As a result, it can be seen that the SOD-like activity of the complex is significantly higher than that of the control vitamin C at a concentration of 5-50㎍/mL. In particular, at a concentration of 50 μg/mL, it was measured to be about 2.5 times larger than that of Vitamin C.

Experimental Example 2. In vitro atopic dermatitis inhibition efficacy measurement of the composite according to the present invention

In order to measure the anti-atopic efficacy of the complex of wild strawberry leaf extract, phloretin, and Edelweiss callus culture extract according to the present invention, the effect on survival and proliferation of RAW264.7 cells, a mouse macrophage cell line, TFN-γ, TNF-α , The effect on IL-4 cytokine expression was tested.

2-1. Effect on cell survival and proliferation

As a result of examining the effect of the complex on the survival and proliferation of RAW264.7 cells, a mouse macrophage cell line, it was confirmed that the cytotoxic and proliferation inhibitory effects were observed at 1000ppm of the complex.

2-2. Effects of TFN-γ, TNF-α, and IL-4 cytokine expression

To investigate the effect of the complex on the expression of TFN-γ, TNF-α, and IL-4 cytokine in RAW264.7 cells, a mouse macrophage cell line, we confirmed the effect on the expression of cytokines by stimulating RAW264.7 cells with LPS. I did.

As a result, it was confirmed that the expression was inhibited at 50 ppm and 100 ppm of the complex.

Experimental Example 3. In vitro inflammation inhibitory activity measurement of the complex according to the present invention

In order to measure the in vitro inflammation inhibitory activity of the complex of wild strawberry leaf extract according to the present invention, phloretin, and culture extract of Edelweiss callus, TNF-α expression by antigen-antibody reaction was confirmed in RBL-2H3 cells.

As a result, it was confirmed that expression was suppressed at 50 ppm and 100 ppm of the complex.

Experimental Example 4. In vitro allergic reaction inhibition activity measurement of the complex according to the present invention

In order to measure the in vitro allergic reaction inhibitory activity of the complex of wild strawberry leaf extract according to the present invention, phloretin and edelweiss callus culture extract, the secretion of β-hexosaminidase and histamine on the inhibitory effect on burnt granules, an indicator of the immediate allergic reaction. It was examined by measuring.

4-1. β-hexosaminidase release assay

As a result of examining the effect of the complex on the secretion of β-hexosaminidase by the antigen-antibody reaction, it was confirmed that the inhibitory effect was 29.8% at 50 ppm and 45.8% at 100 ppm, indicating a significant inhibitory effect in a concentration-dependent manner. I did.

4-2. histamine release assay

As a result of examining the effect of the complex on the secretion of histamine by antigen-antibody reaction, it was confirmed that the inhibitory effect was 40.8% at 50ppm and 54.0% at 100ppm, indicating a significant inhibitory effect in a concentration-dependent manner.

Experimental Example 5. Verification of efficacy in an in vivo anti-atopic animal model of the complex according to the present invention

In order to verify the efficacy of the complex in an in vivo anti-atopic animal model, the complex was treated after inducing contact dermatitis in hairless mice using Oxazolone.

As a result, it was confirmed that contact dermatitis was improved in the experimental group treated with 50 ppm and 100 ppm of the complex.

Therefore, the combination of the wild strawberry leaf extract of the present invention, phloretin and edelweiss callus culture extract prevents skin oxidation to prevent aging and improves skin clarity, and thus has antioxidant, anti-inflammatory, anti-allergic and anti-atopic effects. It was confirmed that it can be used in cosmetics to improve transparency.

As described above, the present specification has been disclosed with respect to a preferred embodiment of the present invention, and although specific terms are used, this is only used in a general meaning to easily explain the technical content of the present invention and to aid understanding of the present invention. It is not intended to limit the scope of the invention. In addition to the embodiments disclosed herein, it is apparent to those of ordinary skill in the art that other modified examples based on the technical idea of the present invention may be implemented.

Claims (8)

A cosmetic composition having an antioxidant effect, characterized in that it contains wild strawberry leaf extract, floretin, and edelweiss callus culture extract.
The method of claim 1,
A cosmetic composition having an antioxidant effect, characterized in that it contains 0.01 to 10% by weight of a composite consisting of the wild strawberry leaf extract, floretin, and edelweiss callus culture extract based on the total weight of the cosmetic composition.
The method of claim 2,
The content of the wild strawberry leaf extract is 1 to 90% by weight based on the total complex, the content of phloletin is 1 to 90% by weight based on the total complex, and the content of the Edelweiss callus culture extract is based on the total complex. Cosmetic composition having an antioxidant effect, characterized in that 1 to 90% by weight.
The method of claim 2,
The mixing ratio (% by weight) of the wild strawberry leaf extract, the phloretin, and the Edelweiss callus culture extract to form a complex is a wild strawberry leaf extract: Phloretin: Edelweiss callus culture extract = 1:1 (% by weight) or 1.5 A cosmetic composition having an antioxidant effect, characterized in that: 1: 1.5 (% by weight) ratio.
The method according to any one of claims 1 to 4,
Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion , A cosmetic composition having an antioxidant effect, characterized in that it is implemented in one or more formulations selected from the group consisting of a formulation of a cleansing cream, a body lotion and a body cleanser.
A cosmetic composition having an anti-inflammatory effect comprising the wild strawberry leaf extract of any one of claims 1 to 4, floretine, and the culture extract of Edelweiss callus as active ingredients.
A cosmetic composition having an anti-allergic effect containing the wild strawberry leaf extract of any one of claims 1 to 4, floretin, and the culture extract of Edelweiss callus as active ingredients.
A cosmetic composition having an anti-atopy effect comprising the wild strawberry leaf extract of any one of claims 1 to 4, floretin, and the culture extract of Edelweiss callus as active ingredients.