KR101184427B1 - Cosmetic composition for improving skin using Gombo-Baechu and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin and a method for manufacturing the same, and more particularly, to a cosmetic composition having an effect of anti-aging, whitening, wrinkle improvement, etc., containing an extract of bark cabbage having excellent antioxidant activity, and a method for preparing the same. will be.
Cosmetic composition for skin improvement using the morel cabbage of the present invention comprises morel extract as an active ingredient. In addition, the cosmetic composition of the present invention further comprises herbal extracts extracted from three hundred seconds, sculptors, gages, cheongung as an active ingredient.

Description

Cosmetic composition for improving skin using the morel cabbage and its manufacturing method {Cosmetic composition for improving skin using Gombo-Baechu and manufacturing method

The present invention relates to a cosmetic composition for improving skin and a method for manufacturing the same, and more particularly, to a cosmetic composition having an effect of anti-aging, whitening, wrinkle improvement, etc., containing an extract of bark cabbage having excellent antioxidant activity, and a method for preparing the same. will be.

The skin is the largest organ in the body and is always in direct contact with the external environment and acts as a protective shield to protect the living body from various irritation or dry environments. It is an organ in which new cells are actively produced and destroyed compared to other organs. In addition, it plays a very important role in protecting the body from physical abrasions, preventing the loss of moisture inside the body, protecting it from the ultraviolet rays generated from the sun, and controlling the body temperature.

When the skin is subjected to external stimulation due to various physical factors and external environmental factors, aging such as wrinkle formation, loss of elasticity and keratinization occurs. Skin aging is largely divided into natural aging (or endogenous aging) and external aging. Natural aging is difficult to artificially control because it is influenced by genetic factors, whereas external aging is relatively easy to control because external aging is affected by environmental factors. Do.

Representative external aging factors include ultraviolet rays, and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch.Dermatol., 1992, 128, 347). One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298).

Free radicals are known to cause breakdown of connective tissue formation such as skin collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein function, intercellular energy transfer, and modification of molecules related to metabolism (Lavker RM and Kligman AM, J. Invest.Drmatol., 1988, 90, 325). The fact that free radicals are involved in aging means that antioxidants or various chemical scavengers that inactivate the free radicals can be used to delay the aging process.

On the other hand, even in normal cells, some degree of free radicals and other free radicals and peroxides are generated during metabolism, but superoxide dismutase (SOD) and catalase as defense mechanisms in vivo are produced. Along with antioxidant enzymes, such as peroxidase, antioxidants such as vitamin C, vitamin E, glutathione, ubiquinone, and uric acid are present to protect themselves. However, oxidative stress is caused when an abnormality occurs in such a biological defense mechanism or when the generation of active oxygen exceeds the capacity of the biological defense system by various physical and chemical factors. Therefore, inhibition of free radicals is an important factor in skin aging cosmetics.

Antioxidants such as tocopherol, polyphenols, Coenzyme Q 10, BHT, and BHA are widely used for the purpose of neutralizing these active oxygen species, but most of them are synthetic products, and their use is limited due to safety issues when applied to human skin for a long time. . As a result, many studies have been made in anticipation of the development of natural antioxidants of plant origin, which have high antioxidant effects, are guaranteed to be safe for human skin, and are economical.

 Since many plant species contain various polyphenols having excellent plant protection effects, numerous plant extracts such as green tea, lettuce, and gold are widely used in cosmetics as antioxidants. Natural antioxidants known to date include tocopherols, flavonoids, gossypol, sesamol, oryzanol, vitamin C, and the like.

On the other hand, Salvia plebeia is a perennial herb of Lamiaceae, also called Seolcho, Cheongwacho, Mamacho, Dongdongcheong, and Squid. In winter, the leaves are attached to the ground and resemble cabbage. Wrinkled and rugged shapes resemble bears on their backs.

Morel cabbage has a vivid aroma, tastes spicy and bitter, cool and nonpoisonous, and is known to be particularly effective for asthma. Ingredients are known to contain phenolic substances, essential oils, saponins, cardiac glycosides, unsaturated fatty acids, etc., in addition to flavonoids such as homoplanetamine, hispiduline, eucaponolin, and eucaponolin-7-glucoside. Chinese cabbage research is still inadequate.

Accordingly, the present inventors have completed the present invention by focusing on the fact that the bark pear supplement contains ingredients beneficial for antioxidants such as polyphenols, flavonoids, etc. during the research for finding a plant species having a skin improvement effect.

Accordingly, an object of the present invention is to provide a cosmetic composition and a method for preparing the same, which have an anti-aging effect, whitening, and wrinkle improvement by using an extract of red pepper which is excellent in antioxidant activity and can be used safely without irritation to the skin. .

Skin improvement cosmetic composition using the morel cabbage of the present invention for achieving the above object is characterized in that it comprises morel extract as an active ingredient.

The cosmetic composition is characterized in that it further comprises a herbal extract extracted from three hundred seconds, engraver, gyeji, cheongung as an active ingredient.

The Morel cabbage extract is characterized in that extracted by adding an extraction solvent to the fermentation broth fermented morel cabbage.

And a method for producing a cosmetic composition for improving skin using the morel bark cabbage of the present invention for achieving the above object is a) extracting the morel cabbage cabbage extract by adding at least one extractant selected from water, alcohol to the morel cabbage; ; b) Add the morel cabbage extract to 0.01 to 20% by weight based on the total weight of the composition, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam It is characterized in that it comprises a; preparing to any one of the formulation selected from hair oil, hair shampoo and hair rinse.

The a) step is to filter the hot water extract obtained by adding 2 to 20 times the weight ratio of water to the dried persimmon cabbage after boiling for 1 to 20 hours and then concentrated the supernatant obtained by centrifugation to extract the persimmon cabbage extract It is characterized by.

Step a) is an ethanol extract obtained by adding 2 to 20 times the weight ratio of ethanol to the dried persimmon cabbage for 1 to 20 hours, and adding 2 to 20 times the weight ratio of glycerol to the dried persimmon cabbage for 10 days. The supernatant obtained by centrifuging the glycerol extract obtained by cold soaking for 20 days and the glycol extract obtained by cooling 2 to 20 times by weight of glycol to the dried bark cabbage for 10 to 20 days were concentrated. It is characterized by extracting morel cabbage extract.

As described above, according to the present invention, it is possible to provide a cosmetic composition which can be used safely without irritation to the skin by containing an active ingredient extracted from a botanical cabbage which is a natural plant.

In addition, the antioxidative effect of protecting the skin from free radicals and free radicals to enhance the skin's physiological activity is expected to be effective in improving the skin, such as anti-aging, whitening, anti wrinkle.

And the morel extract from fermentation broth fermenting the juice of morel cabbage is excellent moisturizing effect.

1 is a photograph showing the antimicrobial activity.

Hereinafter, the cosmetic composition for improving the skin containing the morel cabbage extract according to a preferred embodiment of the present invention and a manufacturing method thereof will be described in detail.

The cosmetic composition of the present invention includes an extract of Morel cabbage extracted from Morel cabbage ( Salvia plebeia ) as an active ingredient.

In the present specification, Morel cabbage extract means that obtained by extracting from various organs or parts of Morel cabbage, preferably means an extract extracted from at least one selected from leaves, roots, seeds.

Morel cabbage is preferably harvested before the flower beds rise between February and March, and can be used after washing the foreign matter attached to the surface of the harvested cabbage cabbage.

Morel cabbage extract can be extracted in various ways. For example, after adding the extraction solvent 2 to 20 times by weight with respect to the morel cabbage cabbage may be used for hot water extraction, cold needle or warm needle extraction for 10 to 150 ℃ for 1 to 20 hours. In addition, in the case of cold or hot extraction can be extracted longer than the time. For example, extraction solvent is added for 10 to 20 days. Further, a reflux cooling extraction method, an ultrasonic extraction method, or the like can be used. In addition to the above-described extraction method, it includes an extract that has undergone a conventional purification process. For example, the active fraction obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value and separation by various chromatography, is also included in the extract.

At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, Butylene glycol, propylene glycol, pentylene glycol, glycerol, etc. can be used as a polyhydric alcohol. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.

In addition, the supernatant obtained by filtration by centrifugation may be used by filtration of the extract extracted using the extractant. In addition, the extract may be obtained in powder form through freeze-drying, spray drying, etc. after passing through a conventional vacuum concentration.

In addition, after mixing 1 g of the dry sample pulverized by the reflux cooling extraction method and 25 mL of 80% ethanol into a round bottom flask, the water bath temperature of the reflux extraction device with a cooling tube was adjusted to 60 ℃ for 6 hours After extraction, the extract was filtered under reduced pressure with a vacuum pump using filter paper, and the remaining residue was repeatedly extracted two or three times in the same manner.

Another form of Morel Cabbage Extract is obtained from fermented broth from which the Morel Cabbage is fermented. To this end, sugar is mixed with the juice obtained by juice of the morel cabbage. At this time, the mixing ratio of 20 to 40% by weight per 100 parts by weight of the juice is mixed into the fermentation vessel. In addition, the scavenger is added to the juice-mixed mixture and inoculated with fermentation bacteria. After inoculation, it is stored at 20-30 ° C. for 2-4 months to ferment.

Fermentation temperature and fermentation period are closely related. If fermentation is carried out at high temperature exceeding 30 ℃, it becomes a good environment for microorganisms to grow regardless of the type, and there is a possibility of deterioration or decay by harmful microorganisms. In addition, since the fermentation broth is not sufficiently fermented at a temperature of less than 20 ° C., a desired fermentation broth cannot be obtained, resulting in a long fermentation period. Preferably, the mixture filled in the fermentation vessel during the fermentation is stirred once or twice. This is to allow the mixture to ferment evenly throughout.

The juice is obtained by compressing the morel cabbage using a juicer and then filtering. At this time, the residue remaining after filtering may be lyophilized or hot air dried to be pulverized in a grinder to obtain residue powder. The fermentation vessel is preferably used onggi. As the sugar, oligosaccharides, lactose, glucose, fructose, sugar, etc. may be used alone or in combination.

The mica is added 8 to 12 parts by weight based on 100 parts by weight of the juice, fermented bacteria inoculate 0.01 to 0.03 parts by weight based on 100 parts by weight of juice. As fermentation bacteria, yeast, yeast or lactic acid bacteria can be used. Saccharomyces cerevisiae can be used as the yeast. The fermentation process is an example of fermentation by artificially inoculating fermentation bacteria, but the fermentation bacteria naturally present in the air or raw materials can be fermented into the fermentation vessel.

Sericite is a type of mica mineral belonging to the monoclinic family and has a white or off-white pearl luster. Originally meant crystalline schist rock, especially the main component mineral of the biotite schist, it also refers to the clay-shaped muscovite produced by hydrothermal action today.

The chemical composition of the biotite is composed of oxygen atoms supporting potassium atoms as peaks, Al, Si atoms based on them, and atoms such as other metals and hydrogen to form an extremely stable crystal structure of the dorsal mica. Is known to be an unspecified crystal formed by irregular substitution of oxygen and potassium atoms. Cicada is a natural inorganic polymer material in which the structure shell of these crystals is arranged in an orderly manner as an arc layer structure, and the hardness has a value of 2 to 2.5, which is softer after gypsum, and the higher the purity, the higher the whiteness and the more natural. The bulk rather than the powder is generally of higher purity.

The biotite component may have a different composition depending on the mining area, but as an example of the biotite applied to the present invention, 54.20 wt% SiO ₂ , 26.70 wt% Al ₂ O ₃ , and Fe ₂ O ₃ 1.03 wt%, CaO 1.10 wt%, MgO 1.66 wt%, K ₂ O 1.57 wt%, Na ₂ O 9.44 wt%.

Preferably, the biotite is pulverized at 150 to 120 mesh particle size and then calcined at 700 to 900 ° C. rather than used as a raw stone form. Due to the high temperature firing, the villi can remove moisture and disintegrate at the same time by dissociating and dehydrating volatile substances.

These mica is a material that has been spotlighted as a raw material of high-grade ceramics, cosmetics and eco-friendly construction because it emits a large amount of far infrared rays and anions and contains various mineral components. In particular, the biotite adsorbs a large amount of metal ions because of its high specific surface area, surface charge and cation exchange ability, and this adsorption characteristic has the effect of reducing or eliminating the concentration of heavy metals in the fermentation broth. In addition, it is possible to increase the fermentation efficiency by absorbing substances that may be generated due to pollution prevention and abnormal fermentation by various germs that may occur during fermentation.

After the fermentation process is completed, the fermentation broth is added to the fermentation broth 30-60% as an alcohol and left to stand for 20 to 28 hours. After standing for 20 to 28 hours, grind finely with a grinder again, homogenize, and centrifuge to take a supernatant, and the supernatant is obtained by removing alcohol components through a conventional vacuum concentration.

The cosmetic composition of the present invention preferably contains the Morel cabbage extract in an amount of 0.01 to 20% by weight based on the total weight of the composition. If the morel extract is less than 0.01% by weight, the antioxidant effect is insignificant, if it exceeds 20% by weight it is difficult to have an elevated effect compared to the content.

And the cosmetic composition of the present invention may further comprise a herbal extract in addition to the Morel cabbage extract as an active ingredient. Such herbal extracts increase the antioxidant effect of the cosmetic composition of the present invention. Herbal extract may contain from 0.01 to 10% by weight relative to the total weight of the cosmetic composition.

Herbal extracts include Yeongyo, Cheongung, Creation, Geumgumhwa, Indongcho, Gosam, Sambaekcho, Windproof, Sewao, Baeksunpi, Dongguk, Gyeji, Baekjak, Hwangyeon, Iris, Siegyeop, Sculptor, Yeoksil, Hyeonggae, Safflower, Rhubarb, Licorice, Phosphorus, It is extracted from at least one selected from the group consisting of gold and true. Preferably 300 seconds, sculptors, gages, cheongung that can greatly improve the antioxidant effect is used. The extraction method may be applied in the same manner as the above-mentioned extraction method of Morel cabbage extract.

Cosmetic composition of the present invention by adding a variety of well-known ingredients and the like using methods known in the art, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam It may be prepared in any one of the formulations selected from hair oil, hair shampoo and hair rinse.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream or gel, pack, as known components, animal fibers, plant fibers, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide and the like can be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan non-dispersion ester and the like can be used.

Water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester when the formulation of the cosmetic composition of the present invention is a suspension, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.

Aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether sulfate when the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent , Alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters and the like can be used.

In addition to the cosmetic composition of the present invention can be used as a conventional known ingredients such as stabilizers, solubilizers, vitamins, pigments and fragrances, can be prepared in any formulation commonly prepared in the art, Can be appropriately selected accordingly.

As an example of the formulation, the lotion may be 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 3.0% by weight of betaine, 3.0% by weight of diethan panthenol, 1.0% by weight of sebum-A, 2.0% by weight of arbutin paste, 2.0% by weight of anenosine, eco gum It may be composed of 0.03% by weight, glycerin 4.0% by weight, collagen 1.0% by weight, Morel cabbage extract 5.0% by weight, preservatives (trace), purified water (residual amount).

In addition, as an example of the formulation, the lotion may include 1.0 wt% of carbomer, 2.0 wt% of dimethicone, 5.0 wt% of jojoba oil, 0.5 wt% of provitamin E, 1.0 wt% of olive emulsion wax, 5.0 wt% of arbutin paste, and T-mixture. 1.2 wt%, foam mix 1.0 wt%, batine 1.0 wt%, cetearyl alcohol 1.5 wt%, glycerin 4.0 wt%, flex oil 5.0 wt%, arbutin paste 5.0 wt%, Morel cabbage extract 5.0 wt%, blending It may be composed of essence oil (trace amount), preservative (trace amount), purified water (residual amount).

In addition, an example of the formulation of the cream is 1.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 1.0% by weight of polysorbate 60, 0.3% by weight of sorbitassesquioleate, 6.0% by weight of cetyloctanoate, Squalane 8.0 wt%, Apricot kernel oil 4.0 wt%, Dimethicone 2.0 wt%, Butylene glycol 4.0 wt%, Glycerin 4.0 wt%, Magnesium aluminum silicate 0.4 wt%, Morel cabbage extract 5.0 wt%, Blending essence oil (Trace amount), preservative (trace amount), and purified water (residual amount).

In addition, as an example of the formulation, the essence is 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, aqueous sodium hyaluronate solution (1%) It may be composed of 0.03% by weight, Diel panthenol 3.0% by weight, glycerin 4.0% by weight, Sebum-A 1.0% by weight, Morel cabbage extract 5.0% by weight, blending essence oil (trace), preservatives (trace), purified water (residual amount). .

In addition, as an example of the formulation, the foundation may be 2.0% by weight of stearic acid, 0.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 0.5% by weight of polysorbate 60, 0.7% by weight of sorbitan sesquioleate, hydro 2.0% by weight of genelade seed oil, 5.0% by weight of sunflower oil, 6.0% by weight of squalane, 4.0% by weight of cetyloctanoate, 2.0% by weight of cyclomethicone, 5.0% by weight of butylene glycol, 3.0% by weight of glycerin Silicate 0.6%, xanthan gum 0.05%, titanium dioxide 10.0%, talc 3.0%, hydrated ferric oxide 1.3%, ferric oxide 0.3%, ferrus-ferric oxide 0.15%, preservative (trace) , Purified water (remaining amount), and may be composed of 5% by weight of Morel cabbage extract.

In addition, as an example of the formulation, the pack is 0.5 wt% propylene glycol, 1.0 wt% natural liquid, 3.0 wt% polyvinyl alcohol, 3.0 wt% ethanol, 1.0 wt% carbomer, 2.0 wt% collagen, 1.0 wt% eco gum, peptide RF 1.0 wt%, Diel Panthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Morel cabbage extract 5.0 wt%, Blending essence oil (trace), Preservative (trace), Purified water (Remaining amount) It can be composed of).

In addition, as an example of the formulation, the hair shampoo is 0.5 wt% polyquaternium, 1.0 wt% natural liquid, 3.0 wt% cocamidopropyl betaine, 3.0 wt% betaine, 7.0 wt% CDA, LES 25.0 wt%, cocobetaine 5.0 wt%, Collagen 1.0 wt%, Diel Panthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Morel cabbage extract 5.0 wt%, Blending essence oil (trace), Preservative (trace) , Purified water (residual amount).

And it can be understood that the herbal extract in the above-described formulations may be contained 2.0% by weight.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

(Example 1)

The leaves and roots of Morel cabbage collected between February and March were washed thoroughly and then dried naturally to prepare Morel cabbage. 400 g of the prepared Morel cabbage was finely chopped, water was added 10 times by weight, heated at 100 ° C. for 6 hours, and the solids were filtered off and centrifuged at 6000 rpm for 20 minutes to obtain a supernatant. The supernatant was concentrated to reach pH 8 to obtain an extract.

The first test sample was prepared by mixing 5% by weight of the extract and 95% by weight of purified water.

(Example 2)

As in Example 1 was washed finely prepared by washing and dried Morel cabbage. 90% ethanol was added 20 times to the weight of 400 g of cut cabbage cabbage and cooled by immersion at room temperature for 6 hours to obtain an ethanol extract. In addition, glycerol was added to 400 g of cut cabbage cabbage at a weight ratio twice, and cooled at room temperature for 15 days to obtain a glycerol extract. In addition, glycol was added to 400g of the cut Morel cabbage in a weight ratio of 2 times and cooled by cold at room temperature for 15 days to obtain a glycol extract.

The ethanol extract, glycerol extract, and glycol extract were centrifuged at 6000 rpm for 20 minutes to obtain a supernatant, and the supernatant was concentrated to reach pH 8 and then mixed at the same weight ratio to obtain an extract.

5 wt% of the extract and 95 wt% of purified water were mixed to prepare a second test sample.

Example 3

The leaves and roots of the morel cabbages collected between February and March were washed thoroughly and dehydrated. Then, 300 g of ground sugar and 100 g of sorghum were added to the fermentation vessel with 1 kg of juice, followed by 0.02 g of Saccharomyces cerevisiae ( Saccharomyces cerevisiae , KCTC 7107) and then fermented for 3 months at 25 ℃ to obtain a fermentation broth. 60% alcohol was added to the fermentation broth with the same weight ratio and the mixture was allowed to stand for 24 hours. After standing, the mixture was ground finely, homogenized, and centrifuged at 6000 rpm for 20 minutes to obtain a supernatant, and the supernatant was concentrated under reduced pressure to obtain an extract.

5 wt% of the extract and 95 wt% of purified water were mixed to prepare a third test sample.

Example 4

A fourth test sample was prepared by mixing 5% by weight of Morel cabbage extract, herbal extract 2% by weight, and purified water 93% by weight in the same manner as in Example 1.

The herbal extract was added 15 times by weight of water to 500 g of the three hundred seconds, sculptural, gyeji, cheongung purchased from the medicinal market, heated at 100 ℃ for 10 hours, filtered the solid and then centrifuged for 20 minutes at 6000rpm to obtain a supernatant. The supernatant was concentrated to reach pH 8 to obtain an extract.

Experimental Example 1: Skin Irritation Experiment

Skin test was performed to determine the degree of skin irritation for each sample of Examples 1 to 4.

For skin tests, a standard patch (IQ chamber, Chemotechnique, Sweden) containing a sample was attached to the back of 33 men and women (mean age 24.5 ± 2.5 years) and removed 24 hours later and read 30 minutes later. 24h single epicutaneous patch test was performed. The degree of response of the skin was determined as 1-4 points according to the ICDRG (International contact dermatitis research group) evaluation standard table for erythema, edema, crust and shallow ulcer, and recorded as a total average value.

Probability of skin irritation is a mean score of 4.5 or more in 6 or more patients (20% of all subjects). (ICDRG score index 0-1.5: no skin irritation, 1.5-2.5: mild erythema with no skin irritation, 2.5-4.5: mild erythema and edema, further confirmation test required> 4.5: possible skin irritation)

division Skin irritation degree (ICDRG average score index) 1st sample <1.5 2nd test sample <1.5 Third test sample <1.5 4th test sample <1.5

Referring to Table 1, all four samples were found to have no erythema or edema reactions that could cause significant skin irritation in the test subjects that were patched for 24 hours. Thus, when applied to the skin, they can be safely used without irritation.

Experimental Example 2 Antioxidant Activity Test

The results of measuring the radical scavenging activity of the first to fourth test samples are shown in Table 2 below.

The antioxidant donation ability (EDA%) of the samples was measured by modifying Blois' method. 0.5 mL of 0.2 mM DPPH (1,1-diphenyl-2-picryl hydrazyl) was added to 2 mL of the sample solution, stirred for 30 minutes, and the absorbance was measured at 517 nm. The electron donating effect was expressed as the absorbance reduction ratio of the sample solution and the no-added solution. Radical scavenging activity was calculated by the following equation.

Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-added sample) × 100

division Radical scavenging performance 1st sample 53.7 ± 0.65 2nd test sample 55.4 ± 0.40 Third test sample 60.2 ± 0.35 4th test sample 61.5 ± 0.40 Control 41.8 ± 0.45

Looking at the results of Table 2, the first to fourth test samples showed a much higher value than the scavenging capacity of 41.8 ± 0.45 of BHT (synthetic antioxidant) used as a control. It was found that the antioxidant activity of the third test sample and the fourth test sample using the morel cabbage extract extracted from the fermentation broth was better than the first and second. This seems to be the result of the useful components produced by the fermentation process and the useful components contained in the herbal extracts to increase the antioxidant activity of the morel cabbage extract.

Experimental Example 3: Moisturizing and Whitening Experiment

Experiments were conducted to confirm the moisturizing effect and whitening of the first and fourth test samples. For this purpose, 20 male and female adults were selected. The ages ranged from 20 to 50 years with 8 males and 12 females. The test subjects were divided into four groups of five people, using the first test sample in the first group, the second test sample in the second group, the third test sample in the third group, and the fourth test sample in the fourth group. It was.

Subjects who participated in the measurement test acclimated to the temperature and humidity conditions of the evaluation laboratory at least 30 minutes before each measurement was performed. Samples were distributed before the evaluation test and trained to be applied twice a day to designated skin areas. The amount applied once was evaluated after one month by applying each sample uniformly at a level of 0.5 ml / cm 2. The results are shown in Table 3 below as average values.

division Moisturizing effect Whitening effect Group 1 4.1 4.3 2nd group 4.0 4.1 3rd group 4.7 4.1 4th group 4.5 4.4

(1: Very bad, 2: Bad, 3: Normal, 4: Good, 5: Very good)

Looking at the results of Table 3, looking at the average value for the moisturizing and whitening effect of the site where each sample was applied it can be confirmed that the present invention has excellent moisturizing and whitening effect. In particular, the third group using the third test sample showed the highest moisturizing effect, which can be seen that significantly increases the skin moisturizing effect when using fermentation broth.

Experimental Example 4 Antibacterial Test

The antimicrobial effect on the first to fourth test samples was tested. paper disc method of Gram-positive bacteria Staphylococcus epidermidis by using a (Staphylococcus Epidermis ) was confirmed to inhibit the growth.

The result was shown to the photograph of FIG. In Figure 1 (a) (b) (c) (d) is a photograph of the first, second, third and fourth test samples, respectively. As shown in FIG. 1, the first test sample 35 mm, the second test sample 15 mm, the third test sample 17 mm, and the fourth test sample 12 mm were found to have antimicrobial activity. Therefore, the cosmetic composition of the present invention is expected to have an effective antibacterial activity to reduce inflammation of the skin and to remove the itch due to the anti-inflammatory effect on sensitive skin or atopic dermatitis.

In the above, the present invention has been described with reference to one embodiment, which is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments are possible.

Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (6)

delete Contains morel cabbage extract as an active ingredient,
Cosmetic composition, characterized in that it further comprises a herbal extract extracted from three hundred seconds, sculptural, gyeji, cheongung. The cosmetic composition according to claim 2, wherein the extract of Morel cabbage is extracted by adding an extraction solvent to a fermentation broth fermenting the morel cabbage.




delete delete delete

Images