KR102032298B1 - Cosmetics composition for improving skin-whitening, anti-wrinkle and head skin comprising selenium as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving scalp conditions comprising selenium as an active ingredient. The cosmetic composition for improving scalp conditions of the present invention comprises selenium as an active ingredient, wherein selenium is included in the weight ratio of 0.01 to 5 wt% based on the total content of the cosmetic composition. By the configuration, the present invention can soothe the scalp and improve the scalp conditions to have a healthy scalp and hair.

Description

Cosmetic composition for skin whitening, wrinkle improvement and scalp health improvement containing selenium as an active ingredient {COSMETICS COMPOSITION FOR IMPROVING SKIN-WHITENING, ANTI-WRINKLE AND HEAD SKIN COMPRISING SELENIUM AS AN ACTIVE INGREDIENT}

The present invention relates to a cosmetic composition for improving scalp health comprising selenium as an active ingredient, and more particularly, by preparing a cosmetic composition comprising selenium as an active ingredient, so as to calm the scalp and have a healthy scalp and hair. It relates to a cosmetic composition for improving scalp health containing selenium as an active ingredient.

In modern society, free radicals are generated by ultraviolet rays, water pollution, etc. due to destruction of exhaust gas, harmful chemicals and ozone layer, and these free radicals penetrate into human body and cause various side effects, especially pigment change and skin aging. It is known to be the main cause.

In addition, in recent years, outdoor leisure activities such as golf are increasing to relieve excessive social and work stress. During leisure activities, such skin damages the skin because body skin is more easily directly exposed to the pollutants. In addition, the aging of the skin is inevitable, and it has a negative effect on the trend of more active and pleasant enjoyment of outdoor leisure activities.

In general, the skin plays a primary role in preventing metabolism from various external factors that impair our bodies' homeostasis through basic metabolic processes. In other words, the skin is located at the outermost part of the body and plays various roles such as protection through physical, chemical and biological barrier functions, thermoregulation, sensory activity, secretion and excretion, absorption, storage and regeneration. However, they are most susceptible to damage when constant external stress is applied.

The main causes of such external stress are ultraviolet exposure, exhaust gas, yellow dust, environmental pollutants, heavy metals, temperature changes, and microorganisms, and these are mainly reactive oxygen species through oxidative stress. , ROS), disrupts antioxidant defenses, causing cell damage and death.

In general, the phenomenon of skin wrinkles due to the weakening of the elasticity and contractility of skin cells is caused by a variety of causes, it is widely known that skin aging reduces the shrinkage, astringent action of the skin.

The aging of the skin is largely classified into internal factor aging and external factor aging. Internal factor aging is an aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. It is clinically characterized by reduced elasticity, rough skin texture, deep wrinkles and pigmentation.

In addition, external factor aging refers to an aging phenomenon caused by exogenous factors such as ultraviolet rays, reactive oxygen species, and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

Various studies have been conducted to investigate the aging mechanism of the skin as described above, and these studies have revealed various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, Skin physiological activity as a protective barrier is lowered and aging is promoted. When skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and it causes deformation of various proteins constituting the skin. Aging progresses.

This aging of the skin and a decrease in elasticity are caused by a decrease in the production and destruction of collagen and elastin due to the weakening of skin cells (fibroblasts) due to ultraviolet rays and the like. Collagenase and elastase are expressed in the skin by ultraviolet rays, and this enzyme breaks down normal collagen and elastin in the skin, resulting in decreased skin elasticity.

On the other hand, selenium is an essential component of humans and animals and is a component of glutathione peroxidase, an important component of antioxidant enzymes that protect cells from free radicals generated during oxygen-consuming metabolism. It is essential for the normal functioning of the immune system and thyroid gland.

In addition, selenium has the effect of preventing aging, reducing the incidence of cancer such as prostate cancer, reducing cardiovascular disease, preventing liver damage caused by alcohol, and enhancing fertility in men, if insufficient, causing abnormalities in the brain and heart muscle, Kesan-disease, a type of heart disease, or Kashin-Beck disease, a joint disease, can result.

Accordingly, the present inventors help to easily respond to skin whitening, anti-aging, wrinkle improvement, soothing the scalp, and as a result of trying to develop a cosmetic composition using a material that is safe for the human body, including selenium as an active ingredient. By preparing a cosmetic composition, it was confirmed that it can soothe the scalp, have a healthy scalp and hair, enhance skin whitening and elasticity of the skin, and improve wrinkles of the skin.

Domestic Registration Patent No. 10-1646569 (registered 08/02/2016) Domestic Patent Publication No. 10-2012-0082498 (published Jul 24, 2012) Domestic Patent No. 10-1702482 (registered Jan. 26, 2017)

The present invention is to provide a cosmetic composition for improving the health of the scalp comprising selenium as an active ingredient that can improve the soothing scalp and have a healthy scalp and hair by preparing a cosmetic composition comprising selenium as an active ingredient.

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other tasks not mentioned will be clearly understood by those skilled in the art from the following description.

Scalp health improvement cosmetic composition comprising selenium according to the present invention as an active ingredient includes selenium as an active ingredient, the selenium is contained in a weight ratio of 0.01 to 5% by weight of the total content of the cosmetic composition.

In addition to the selenium, natural extract consisting of oregano extract and green tea extract, oil consisting of Amla oil and Bringgras oil, zinnia extract, copper tripeptide-1, raspberry extract (Rubus) Idaeus (Raspberry) Fruit Extract, iris extract (Iris Ensata Extract), hyaluronic acid, zinc gluconate (Zinc Gluconate), magnesium gluconate (Magnesium Gluconate) and may further include an ox powder.

0.01 to 5 parts by weight of the selenium, 0.1 to 3 parts by weight of the natural extract consisting of oregano extract and green tea extract, 0.1 to 0.8 parts by weight of oil consisting of Amla oil and bringraj oil, baeknyeoncho extract 0.5 to 1.5 Parts by weight, 0.01 to 0.05 parts by weight copper tripeptide-1, 0.15 to 0.25 parts by weight of Raspberry Idaeus (Raspberry) Fruit Extract, 0.15 to 0.25 parts by weight of Iris Ensata Extract , 0.001 to 0.005 parts by weight of hyaluronic acid, 0.001 to 0.005 parts by weight of zinc gluconate, 0.003 to 0.008 parts by weight of magnesium gluconate, and 0.005 to 0.025 parts by weight of ox powder.

The natural extract may be included in a weight ratio of 1 to 3 parts by weight of oregano extract and 2 to 4 parts by weight of green tea extract, and the oil may be included in a weight ratio of 2 to 4 parts by weight of Amla oil and 3 to 6 parts by weight of Bringraz oil. have.

Specific details of other embodiments are included in the detailed description.

Cosmetic composition comprising selenium according to the present invention as an active ingredient can be improved so as to soothe the scalp and have a healthy scalp and hair.

In addition, the cosmetic composition comprising selenium according to the present invention as an active ingredient can soothe the scalp and relieve skin inflammation to improve scalp health, and excellent dandruff and skin irritation effect to have a healthy scalp and hair.

It will be fully understood that embodiments of the inventive concept may provide various effects that are not specifically mentioned.

1A and 2A are photographs showing the scalp state of a subject (38 years old and 48 years old), and FIGS. 1B and 2B are views of a subject after washing hair for 30 days using a cosmetic composition prepared according to Example 1; This picture shows the scalp condition.
Figure 3a is a photograph showing the facial skin condition of the subject (48-year-old woman), Figure 3b is a photograph showing the facial skin condition of the subject after washing for 30 days using the cosmetic composition prepared according to Example 1.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosure may be made thorough and complete, and to fully convey the spirit of the present invention to those skilled in the art.

The terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting of the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

As used herein, the term " improve scalp health " means to calm the scalp and beautify hair to increase attractiveness and to keep hair healthy.

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In addition, the term "skin moisturizing" used in the present invention means maintaining the homeostasis of the living body by appropriately controlling the moisture loss (moisture evaporation) of the skin and the like.

Hereinafter, the present invention will be described in detail with reference to a preferred embodiment of the cosmetic composition for improving scalp health containing selenium according to the present invention.

Scalp health improvement cosmetic composition comprising selenium according to the present invention as an active ingredient comprises selenium as an active ingredient.

The selenium is a trace element (inorganic) that is essential for our body, and this nutrient is an important component of antioxidant enzymes that protect cells from free radicals generated during normal oxygen metabolism process, which is essential for the normal functioning of the immune system. have.

In the present invention, the selenium may be included in a weight ratio of 0.01 to 5% by weight in the total content of the cosmetic composition, when the selenium is contained in less than 0.01% by weight, it is not possible to obtain an effect containing selenium such as an antioxidant effect, 5 When included in an amount exceeding%, problems of skin irritation may occur.

In addition, the cosmetic composition according to the present invention is a natural extract consisting of oregano extract and green tea extract in addition to the selenium, oil consisting of Amla oil and bringraj oil, zinnia herb extract, copper tripeptide (copper tripeptide) -1), raspberry extract (Rubus Idaeus (Raspberry) Fruit Extract), Iris Ensata Extract, hyaluronic acid, zinc gluconate, magnesium gluconate and ox powder Can be.

In addition, the cosmetic composition according to the present invention is 0.01 to 5 parts by weight of the selenium, 0.1 to 3 parts by weight of the natural extract consisting of oregano extract and green tea extract, oil consisting of Amla oil and Bringraj oil (bhringraj oil) 0.1 to 0.8 parts by weight, baeknyeoncho extract 0.5 to 1.5 parts by weight, copper tripeptide-1 0.01 to 0.05 parts by weight, raspberry extract (Rubus Idaeus (Raspberry) Fruit Extract) 0.15 to 0.25 parts by weight, iris Iris Ensata Extract 0.15 to 0.25 parts by weight, hyaluronic acid 0.001 to 0.005 parts by weight, zinc gluconate 0.001 to 0.005 parts by weight, magnesium gluconate 0.003 to 0.008 parts by weight and ox powder 0.005 to It may be included in the weight ratio of 0.025 parts by weight.

In addition, the natural extract may be included in the weight ratio of 1 to 3 parts by weight of oregano extract and 2 to 4 parts by weight of green tea extract, the oil is a weight ratio of 2 to 4 parts by weight of Amla oil and 3 to 6 parts by weight of Bringgraz oil It may be included as.

The oregano extract means that it is extracted from oregano (Oregano or Origanum vulgare), the oregano (Oregano or Origanum vulgare) is the origin of the Mediterranean coast and was used medicinally in ancient Greece and used in cooking in ancient Rome. Edo is a natural preservative for meat storage, and is used as a spice in Italian cuisine.

Oregano, also known as a natural antibiotic, is said to have been effective in tonic, diuretic, dry stomach, appetite, soothing, antiseptic and headache, motion sickness and insomnia since ancient Greek times. helpful.

For example, oregano extract used in the present invention may be used oregano extract prepared by the following manufacturing method.

First, the oregano stem and oregano raw leaves can be separated and prepared and washed.

Next, the washed oregano stem may be dried, and the drying may dry the washed oregano stem at a temperature of 25 to 30 ° C. for 5 to 10 days.

Next, the dried oregano stem may be cut in a predetermined length unit, for example, the dried oregano stem may be cut in a length unit of 0.5 to 2.5 cm.

Subsequently, the cut oregano stem may be mixed with lactic acid bacteria and then aged. The ripening may be performed by maintaining the oregano stem mixed with the lactic acid bacteria for 20 to 30 hours at a temperature of 28 to 32 ° C. and a humidity of 50 to 55%. You can.

For example, any one known lactic acid bacteria selected from the group consisting of Lactobacillales , Streptococcus thermophiles , and Bifidobacterium longum may be used as the lactic acid bacteria.

Next, the washed oregano raw leaves can be heated with steam, wherein the heating is performed by heating the washed oregano raw leaves with steam for 10 to 30 minutes at a temperature of 125 to 135 ° C. and a pressure of 2 to 3 kgf / cm ² . Can be.

Next, the raw oregano leaves heated with water vapor may be dried, and the drying may be carried out by drying the raw oregano leaves at a temperature of 20 to 25 ° C. to prevent chlorophyll of the raw oregano leaves from being dried and volatilization of useful components. Can be dried for 20 to 40 hours.

Subsequently, the dried oregano raw leaves may be heated and squeezed. The squeezing may be performed by putting the dried oregano raw leaves into a heating vessel (for example, a cauldron) at a temperature of 70 to 80 ° C. for 3 to 5 minutes. Then, the first steamed oregano raw leaves may be proceeded to the second step of 20 to 50 seconds at a temperature of 110 to 120 ℃.

Next, after mixing in a weight ratio of 200 to 400 parts by weight of the chopped oregano raw leaves with respect to 100 parts by weight of the aged oregano stem, aged for 5 to 10 days at a temperature of 55 to 60 ℃ and humidity of 55 to 60% It can be fermented to produce oregano fermented products.

Subsequently, the oregano fermentation extract may be prepared by mixing a solvent with the oregano fermentation product and adding ultrasonic waves.

In order to prepare the oregano fermented extract, the oregano fermented product is mixed with a solvent to prepare a mixed solution, and then an ultrasonic extractor is added to the mixed solution to prepare an oregano fermented extract, and the solvent is the oregano mixed solution. It is included in the weight ratio of 400 to 600 parts by weight based on 100 parts by weight, water may be used as the solvent.

In addition, the ultrasonic wave applied to the mixed solution to prepare the oregano fermentation extract can be extracted using an output of 100 to 200 watts (watt) for 50 to 100 minutes at a vibration frequency of 60 to 80KHz.

Next, in the oregano fermentation extract, solids such as oregano stems and raw oregano leaves may be removed, and separation of solids such as oregano stems and oregano raw leaves may use a well-known strainer such as a sieve.

Next, the oregano fermented extract from which the solids have been removed may be centrifuged.

In the step, by centrifuging the oregano fermented extract from which the solids are removed, the oregano stem and the raw leaf microparticles can be separated and separated into a lower layer solution and a supernatant and a supernatant located above the lower layer solution. have.

Next, the oregano fermentation extract located in the supernatant and the supernatant may be extracted to prepare an oregano extract containing useful components of oregano.

In the step, the oregano extract is first extracted with 70% (v / v) ethanol aqueous solution 10-15 L in an oregano fermented extract 2000 to 3000g for 3 to 4 hours at 85 to 90 ℃, 70% (v / v) ethanol Completing the second extraction for 2 to 3 hours at 85 to 90 ° C. with an aqueous solution of 4 to 6 l, and preparing an extract until the amount is 1000 to 1500 ml in a vacuum rotary evaporator; And ethanol was added to the extract to 2 to 3 times the weight, and centrifuged at 18,000 to 20,000 rpm for 30 to 40 minutes to completely remove the polymer fibers and solids that may be included in the oregano fermentation extract, and then collect the collected supernatant 1.5. It can be prepared by the step of extracting twice with ˜2.0 times the weight of acetic acid.

The green tea extract can be extracted from the seeds of the tea tree (Camellia sinensis), 30 to 40% of the polyphenols found in the green tea is known as flavonol and catechin components, the main component of green tea catechin is epicatechin (epicatechin) , Epicatechin-3-gallate, epigallocatechin, epigallocatechin-3-gallate (EGCG), and epigallocatechin- It is known that the content of the 3-gallate component is the highest, the polyphenol component of the green tea is known to have a variety of efficacy effects, such as antioxidant, anticancer, anti-inflammatory, antibacterial.

In the present invention, the green tea extract may be used the green tea extract prepared by the following manufacturing method.

First, after preparing and washing the green tea leaves, the green tea leaves may be dried for 10 to 20 hours in a dryer maintained at a temperature of 23 to 27 ℃ and humidity 40 to 45%.

Next, after grinding the dried green tea leaves can be steamed by heating the crushed green tea leaves with steam.

In the step, the dried green tea leaves are ground to have a particle size in the range of 500 to 3000 μm, and the ground green tea leaves are 30 to 100 minutes at a temperature of 110 to 130 ° C. and a pressure of 2.5 to 3.5 kgf / cm ² . The green tea leaves can be steamed by heating with steam for a while.

Next, after the solvent is mixed with the steamed green tea leaves, ultrasonic waves may be added to prepare green tea ultrasonic extracts.

In the step, after mixing the solvent in a weight ratio of 300 to 600 parts by weight of the solvent with respect to 100 parts by weight of the steamed green tea leaves, an ultrasonic wave may be added to the mixed solution, the ultrasonic wave at a vibration frequency of 60 to 90Khz Extraction can be made using an output of 100 to 200 watts for 20 to 40 minutes. In the above step, any one or more solvents selected from alcohols having 1 to 4 carbon atoms (C1 to C4) may be used as the solvent.

Subsequently, honey may be mixed with the green tea ultrasonic extract and then aged.

In the step, after mixing at a weight ratio of 1 to 3 parts by weight of honey with respect to 100 parts by weight of the green tea ultrasonic extract, it can be aged by storing for 5 to 10 days at a temperature of 30 to 35 ℃, the step is the lower limit If it is carried out less than may cause problems that are not fully aged, if it is carried out above the above upper limit range may not be accompanied by a further significant effect increase over time may cause a problem of increasing the process cost .

The honey has been used for nourishing tonic, soothing, detoxification, fatigue recovery, gastrointestinal disorders, cough, dry skin, eczema, constipation, nervous breakdown, hangover, etc., good for beauty and health, rich in vitamin B group, especially B6 It is.

In particular, honey does not change at a high temperature (30 ~ 40 ℃) because of the strong sterilization power, in the present invention because of the sterilizing power of the honey as described above, even if the aging temperature of the green tea ultrasonic extract aged with honey is not deteriorated easily and stably Green tea ultrasonic extract can be aged.

Next, green tea extract may be prepared by concentrating the aged green tea extract.

In the step, the first extraction of the 75% (v / v) ethanol aqueous solution 15ℓ per 2.0kg of the aged green tea ultrasonic extract for 4 hours at 80 to 85 ℃, and then the second extraction is completed under the same conditions with 7L of the same solvent, Prepare a concentrate until the 1400 to 1600ml in a vacuum rotary evaporator, dilute to 2 to 3 times by adding water to the concentrate, centrifuged at 14,000 to 15,000rpm for 40 to 60 minutes to completely remove the solid component Green tea extract may be prepared by extracting the collected supernatant twice with 1.5 times chloroform, removing the chloroform layer, and extracting the aqueous layer three times with 1.5 times the weight of ethyl acetate.

The Amla oil may be a known oil extracted from Amra, which is a counter-large and deciduous tree commonly known as Indian gooseberry. It is widely distributed in tropical and subtropical countries such as China, India, Indonesia, and Taiwan, and it is known to contain vitamin C, minerals, amino acids, tannins, pilamblic acid, rutin, curcuminoids, embolic, and phenolic compounds. Zhang YJ et al., J Nat Prod, 63: 1507-1510, 2000. Amra contains 20 times more vitamin C than oranges, which are extremely stable to heat and do not destroy vitamins as they are freshly harvested from trees even after prolonged exposure to high temperatures. Amra's vitamin C durability and heat resistance are believed to be derived from tannins. The antioxidant, anti-tumor and anti-inflammatory activity of Amra fruit extracts is known to be due to the high content of vitamin C and polyphenols.

The bringgrass oil has been used as a medicine for enriching hair for centuries in India, and the bringgrass is also known as nasturtium, and the eclipta prostrate is a perennial plant of the asteraceae, which is 10 to 70 cm high. It is a plant that occurs a lot in the soybean fields of Jeju Island, southern part and central part of Korea. It is known to be effective in promoting blood flow, analgesic, immunity, dermatitis, promoting hair growth, and cracking dermatitis. In particular, it is known to be effective in alleviating skin irritation.

The baeknyeoncho extract is prepared by extracting the useful components of baeknyeoncho, the fruit of the baeknyeoncho is rich in minerals, dietary fiber, polar bonoids good for aging prevention, the mineral content of calcium, magnesium and sodium is similar to aloe The content of vitamin C is five times higher than that of aloe, and in particular, the content of dietary fiber is reported to be the highest among all foods.

In other words, the content of phenolic substances and flavonoids was 5% at the beginning of the year, much higher than 1.67% of ginger and 2.21% of root roots, and 30% of dietary fiber, 1.19 ~ 10.35% of grains, and 0.99 ~ 7.42% of fresh vegetables. It is found that it contains much more than 0.19 ~ 2.19% of fruits, and vitamin C is also high.

In addition, baeknyeon fruit contains more phenolic substances and flavonoids than other foods and is known as a diet food, as well as antipyretic calm, bronchial asthma, indigestion, stomach cramps, constipation, chest pain, poor blood circulation, It has been used as a folk medicine for the improvement of gastrointestinal disorders, hind neck pain, rhinitis, degenerative arthritis, headache, insomnia, hypertension, cancer, anti-aging, diabetes and hyperlipidemia.

The baeknyeoncho extract in the present invention may be used baeknyeoncho extract prepared by the following manufacturing method.

In order to prepare the baeknyeoncho extract, first, the harvest of baeknyeoncho 1 to 3 (w / w)% concentration range and sodium hydrogencarbonate (NaHCO ₃ ) having a temperature of 33 to 37 ℃ dissolved for 1 to 5 minutes It can be washed for a while to remove the foreign matter attached to the zinnia, and the zinnia can be separated into zinnia fruit and zinnia stem.

Next, the zinnia fruit is dried in a dryer, and the drying of the zinnia fruit is carried out in a dryer in which a constant temperature and humidity are maintained in order to prevent chlorophyll of the zinnia fruit and the inherent aroma of the zinnia fruit during sun drying. For example, the drying of the zinnia fruit may be dried for 3 to 7 hours in a dryer maintained at a temperature of 23 to 27 ℃ and humidity 38 to 42%.

Next, the dried zinnia fruit can be mixed with purified water and heated to produce the zinnia fruit extract.

The zinnia fruit extract may be prepared by mixing 500 to 700 parts by weight of purified water with respect to 100 parts by weight of the dried zinnia fruit and then heating at a temperature of 90 to 95 ° C. for 5 to 15 hours.

Subsequently, by drying the zinnia stem to remove moisture contained in the zinnia stem, the drying of the zinnia stem may be dried for 5 to 10 hours in a dryer maintained at 33 to 37 ℃ and humidity 40 to 45% , The present invention by drying the baeknyeon stem under the conditions as described above, by gradually evaporating the moisture contained in the baeknyeon stem, it is possible to more easily extract the inherent aroma and useful components of the baeknyeon stem, baeknyeoncho By breaking the stem tissue of the stem facilitates the penetration of air to promote the fermentation effect can be a strong component of the stem of the early 100 years.

Next, the dried zinnia stem may be mixed with purified water and then heated to prepare the baeknyeon stalk extract. The baeknyeon stalk extract may be mixed with 200 to 400 parts by weight of purified water based on 100 parts by weight of the dried zinnia stem. After immersing for 1 to 3 hours at a temperature of 60 to 70 ℃, the purified water immersed in the baeknyeoncho stem is put into a heater, and the purified water immersed in the baeknyeon stems in the heater 120 to 140 ℃ inside the temperature and pressure vessel The centennial stem extract can be prepared by heating for 4 to 8 hours in a pressure of 2 to 3 kgf / cm ² .

The present invention by dipping the baeknyeoncho stem in purified water as described above, can weaken the plant wall of the baeknyeoncho stem to increase the extraction efficiency.

Next, baeknyeoncho extract can be prepared by mixing the zinnia fruit extract and baeknyeon stem extract, the baeknyeoncho extract is prepared by mixing the baeknyeon fruit extract and baeknyeon stem extract in a weight ratio of 7: 3 to 6: 4 Can be.

Subsequently, the zinnia herb extract may be aged by storing for 5 to 10 hours at a temperature of 3 to 7 ° C., and the zinnia herb extract may be uniformly mixed and the fragrance may be enhanced by aging under the same conditions.

The copper tripeptide-1 is a copper complex of tripept-1, and may be used for the purpose of wrinkle improvement, skin conditioner, anti-inflammatory, and the like.

In other words, the copper tripeptide-1 (copper tripeptide-1) improves wound healing ability, enhances immune cell function, and has antioxidant and anti-inflammatory effects. It also promotes collagen synthesis and strengthens blood vessels in skin tissue. As a synthesized peptide, it is used for damage repair and anti-aging components. Aging prevention and firming and anti-wrinkle effect of the copper tripeptide-1 has been confirmed through research through scientific experiments.

In the present invention, the amino acid sequence of the copper tripeptide-1 is a form in which a glycine is coordinated with a copper ion in the form of a histidine, lysine sequence peptide starting with an amino terminal group, and an amino acid on copper (Cu) The sequence can be linked to Gly-His-Lys (GHK).

In the present invention, the copper tripeptide-1 may be prepared using a solid phase peptide synthesis method synthesized using a solid polymer retarder, and the copper tripeptide-1 is prepared using an automatic peptide synthesizer known in the art. Or it can be prepared directly, the synthesis method of the copper tripeptide-1 is a technique known in the art, so a detailed description thereof will be omitted.

The raspberry extract (Rubus Idaeus (Raspberry) Fruit Extract) is produced by extracting from the raspberry, the raspberry (Rubus Idaeus (Raspberry)) is a deciduous shrub and the stem is usually straight. The leaves are alternate, the flowers are white in spring, and the petals and calyx are 5 pieces. Fruits are gathered and ripen in summer, and ripe fruits fall well from flower buds.

The raspberry is not only rich in fiber, but also contains a large amount of effective substances useful for physiological functions of the human body, such as vitamin C and manganese, vitamin A, B, calcium, and potassium.

The iris extract (Iris Ensata Extract) is produced by extracting from the iris, the iris is a perennial herb of the monocotyledonous plant Liliaceae iris family, native to Europe, planted a lot by the pond, short root stem, yellowish brown with roots to be. A stalk is branched and 60 ~ 100cm high. The leaf is about 1m long and the butterfly is 2-3cm.

The irises are used for dry stomach, soothing, analgesic, relieve, expectorant, antifungal, headache, blood circulation, rheumatism, repellent, tonics, fragrances, in particular, diarrhea, bronchitis, indigestion, etc. are used for the roots of irises.

The hyaluronic acid is mainly found in some types of tumors, such as connective tissue such as articular fluid, ocular vitreous fluid, umbilical cord, dermal layer, and Raus sarcoma, in addition to some types of A and C soluble Streptococcus. In bacteria, it is a capillary component. In animal tissues such as bovine cartilage, etc., they are combined with large chondroitin sulfate proteoglycans or binding proteins to form large molecular aggregates. The length of oligosaccharide is 105-106, which is very long, but varies depending on the material, and even the same material is never uniform. It has a property of making gel by combining with a large amount of water, which is connected with functions in vivo such as joint lubrication and skin flexibility.

In addition, when the connective tissue is damaged, the synthesis of hyaluronic acid may be temporarily activated for rapid recovery, and it is considered to form part of cellular activity in tissue reconstruction. CD44 has been found as a hyaluronic acid binding protein on the cell surface. Enzymes for hydrolyzing or cleaving glucosaminide or glucuronide bonds of hyaluronic acid, respectively, have been found from animal tissues such as testes, skin and liver, snake venom, leeches, bacteria and actinomycetes. In addition, when the connective tissue is damaged, the synthesis of hyaluronic acid may be temporarily activated for rapid recovery, and it is considered to form part of cellular activity in tissue reconstruction.

The zinc gluconate is used as a skin conditioning agent and is effective in inflammatory acne due to antiviral properties. In addition, the zinc gluconate is effective to soothe irritated scalp and relieve skin itching.

Magnesium Gluconate is a magnesium salt component of Gluconic Acid, and the chemical formula is C ₁₂ H ₂₂ MgO ₁₄ or C ₁₂ H ₂₂ MgO ₁₄ .2H ₂ O, and has a molecular weight of 414.61. Helps keep you healthy and in good condition.

The ox powder smoothes and smoothes the skin texture, and effectively delivers nutrients to the skin to enhance the moisturizing effect. Jade is roughly divided into jadeite and jadeite, and jadeite is a lead petroleum mineral belonging to the fluoride group. Hardness is a dense mass like crystals, and light is black, cyan, green, transparent or translucent, and jade is commonly referred to as jadeite. Purgatory is a monoclinic whistle mineral of Inosilicate, which is divided into purgatory and serpentine super basic purgates in high-quality surrogate rocks, and their quality is fine with its fine structure, ie, hollow-fiber-Animal sperm, made up of fast and fiber. It is determined to the extent that it is known that the thinner the fiber, the better the quality.

Hereinafter, with reference to the accompanying drawings it will be described in more detail for a preferred embodiment and comparative example for the scalp health improvement cosmetic composition comprising selenium according to the present invention as an active ingredient.

Cosmetic compositions according to Examples 1, 2, 3 and Comparative Examples were prepared to include the components as shown in Table 1 below.

Ingredient (% by weight) Example 1 Example 2 Example 3 Comparative example Selenium 2 4 0.5 - Natural extracts 2 One 3 - oil 0.5 0.7 0.2 0.5 Zinnia extract 1.0 0.6 1.5 - Copper Tripeptide-1 0.03 0.02 0.05 0.03 Raspberry extract 0.2 0.18 0.23 0.2 Iris extract 0.2 0.25 0.16 0.2 Hyaluronic acid 0.003 0.002 0.001 0.002 Zinc gluconate 0.003 0.004 0.001 0.005 Magnesium gluconate 0.005 0.007 0.004 0.009 Ox powder 0.01 0.006 0.023 0.01 Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Here, the natural extract was mixed in a weight ratio of 2 parts by weight of oregano extract and 3 parts by weight of green tea extract, and the oil was mixed in a weight ratio of 3 parts by weight of Amla oil and 5 parts by weight of Bringraz oil.

1. Stability test

The stability test of the cosmetic composition prepared according to Examples 1, 2 and 3 was performed.

Observation period was carried out for the same one week, the stability was observed at various temperatures, the results are shown in [Table 2].

Temperature Example 1 Example 2 Example 3 0 ℃ Good Good Good 40 ℃ Good Good Good -20 ℃ Good Good Good

Referring to the above [Table 2], it was confirmed that the cosmetic composition prepared according to Examples 1, 2, 3 has good formulation stability.

2. Hair Conditioning Effect Test (Sensory Evaluation)

Using a cosmetic composition prepared according to Examples 1, 2, 3 and Comparative Examples, 5 to 20 questionnaires were used for one week, and then 5 points on the questionnaire (very bad, bad, normal). , Good, very good) The quality was evaluated through a questionnaire and the results are shown in [Table 3].

Survey item Example Comparative example One 2 3 Rich and soft foam 4.5 4.4 4.3 2.7 Rinse clean 4.4 4.2 4.2 2.3 Easy to comb when semi-dry 4.5 4.2 4.4 2.2 Soft hair after drying 4.3 4.4 4.2 2.2 The hair is moist after drying. 4.6 4.6 4.5 2.1 Glossiness is felt on the hair after drying 4.6 4.3 4.2 2.4

3. Scalp Stimulation Test

Stimulation clinical trials for the cosmetic composition prepared in Example 1 was carried out as follows.

First, clinical trials were conducted in 20 healthy (8 males, 12 females) between 10 and 50 years old, and no steroids or antibiotics were used from 2 weeks before the end of the experiment. .

And all the subjects were subjected to qualitative evaluation through the evaluation table as shown in Table 4 by observing the scalp state 24 hours after shampooing using the cosmetic composition.

Experiment item Subjective score Evaluation results

Stimulation degree a: The skin is very red and itchy - b: skin is slightly red and itchy - c: skin slightly reddened and irritated - d: skin condition remains unchanged but slightly irritating 1 person e: No irritation or skin condition change 19 people

Referring to [Table 4], it can be confirmed that the cosmetic composition prepared according to Example 1 can be safely used without any change in scalp irritation or skin condition.

4. Skin irritation test of cosmetic composition

In a questionnaire conducted after applying a cosmetic composition prepared according to Example 1 to a face for 2 weeks once a day for 20 women aged 20 to 50 years, skin irritation was evaluated. It is shown in [Table 1].

The following evaluation was carried out using a 5-point scale, which was divided into 0 points-none, 1 points-very mild symptoms, 2 points-obvious symptoms, 3 points-slightly severe symptoms, 4 points-severe symptoms.

division Example 1 (person) Comparative example Erythema formation 0 0 Crust formation 0 0 Edema formation 0 0 Itching 0 0 Acne formation 0 0

Referring to [Table 5], after applying the cosmetic composition prepared according to Example 1 and Comparative Example on the face it was reported that no symptoms such as erythema, itching, edema.

5. Skin elasticity and wrinkle improvement experiment

20 women aged 20 to 50 years were evaluated for skin elasticity and wrinkle improvement in a questionnaire conducted after applying the cosmetic composition prepared according to Example 1 to the face once a day for 2 weeks. The results are shown in the following [Table 6].

The evaluation was evaluated by the 5-point scale method of 1 to 5 points scale, and the higher the score, the better.

division Before use after use Skin moisturizer 2.3 3.4 Skin elasticity 2.2 3.9 Skin wrinkles 2.0 3.5 Skin blemishes, pigmentation 2.1 2.9 Skin tone 2.0 2.5 pore 2.1 3.3

Referring to [Table 6], in the survey conducted after applying the cosmetic composition prepared according to Example 1 to the face once a day for two weeks, 17 of the experimenters skin moisturizing, skin elasticity, skin wrinkles, etc. This improvement was reported to be improved, and 10 of them remarkably improved.

6. Visual assessment of wrinkle improvement

In order to confirm the wrinkle improvement effect of the cosmetic composition according to Example 1, 20 Korean women aged 30 to 50 years old were used in the same manner as the routine lotion use for 8 weeks, and then the wrinkle improvement effect was evaluated.

The cosmetics prepared according to Example 1 were separately used on each side of the face, and the wrinkle improvement effect of the subject was evaluated through visual observation by an experienced doctor.

division Wrinkle improvement effect Effectiveness% Great slightly none Example 1 16 4 - 80 Comparative example 2 5 13 10

Referring to [Table 7], as shown in Example 1, the cosmetic composition comprising selenium, natural extracts, baeknyeoncho extract as compared to the cosmetic composition prepared without containing the components as in the comparative example was confirmed that the wrinkle improvement effect is significantly improved Could.

7. Scalp Absorption Evaluation

The cosmetic composition prepared according to Example 1 and Comparative Example was applied to the scalp to evaluate the degree of absorption.

The degree of absorption was evaluated by sensory evaluation of the degree of absorption after applying the cosmetic 4g to the scalp of 10 subjects, the results are shown in Table 9 below. In addition, the score and evaluation criteria for the sensory evaluation was used a five-point scoring method, the score and evaluation criteria are as shown in Table 8 below.

score Evaluation standard (absorption rate) 5 Very fast 4 speed 3 usually 2 Somewhat slow One Very slow

division Example 1 Comparative example Scalp absorbency 4.4 2.0

Referring to [Table 9], in the case of the cosmetic composition prepared according to Example 1 it was confirmed that the high scalp absorption degree is good formulation feel.

8. Scalp Health Test

After washing the hair of the subject with the cosmetic composition prepared according to Example 1, the scalp state of the subject was observed.

Figures 1a and 2a is a photograph showing the scalp state of the subject (38 years, 48 years old), Figures 1b and 2b is a subject of the subject after washing the hair for 30 days using the cosmetic composition prepared according to Example 1 This picture shows the scalp condition.

1B and 2B, when the hair of the subject was washed using the cosmetic composition prepared according to Example 1, the scalp of the examiner was calmed and the scalp health of the subject was improved.

9. Skin Wrinkle and Whitening Test

After washing the face with the cosmetic composition prepared according to Example 1 (46 year old female), the facial skin condition of the subject was observed.

Figure 3a is a photograph showing the facial skin condition of the subject (48-year-old woman), Figure 3b is a photograph showing the facial skin condition of the subject after washing for 30 days using the cosmetic composition prepared according to Example 1.

3A and 3B, after washing with the cosmetic composition prepared according to Example 1, the subject's wrinkles slightly decreased, and the skin tone was lightened.

As mentioned above, although an exemplary embodiment of the present invention has been described with reference to the accompanying drawings, those skilled in the art to which the present invention pertains may realize the present invention in another specific form without changing its technical spirit or essential features. It will be appreciated that it may be practiced. Therefore, it should be understood that one embodiment described above is illustrative in all respects and not restrictive.

Claims (4)

0.01 to 5 parts by weight of selenium, 0.1 to 3 parts by weight of natural extract consisting of oregano extract and green tea extract, 0.1 to 0.8 parts by weight of oil consisting of Amla oil and bringraj oil, 0.5 to 1.5 parts by weight of zinnia herb extract Parts, copper tripeptide-1 (copper tripeptide-1) 0.01 to 0.05 parts by weight, 0.15 to 0.25 parts by weight of Rubus Idaeus (Raspberry) Fruit Extract, 0.15 to 0.25 parts by weight of Iris Ensata Extract, 0.001 to 0.005 parts by weight of hyaluronic acid, 0.001 to 0.005 parts by weight of zinc gluconate, 0.003 to 0.008 parts by weight of magnesium gluconate and 0.005 to 0.025 parts by weight of ox powder,
The natural extract is included in the weight ratio of 1 to 3 parts by weight of oregano extract and 2 to 4 parts by weight of green tea extract, the oil is included in the weight ratio of 2 to 4 parts by weight of Amla oil and 3 to 6 parts by weight of Bringraz oil. ,
The oregano extract is prepared by separating the oregano stem and raw oregano leaves, and drying the washed oregano stem, the drying is dried oregano stem for 5 to 10 days at a temperature of 25 to 30 ℃, The dried oregano stem is cut in length units of 0.5 to 2.5 cm, and the cut oregano stem is mixed with lactic acid bacteria and then aged, wherein the aging oregano stem is mixed with the lactic acid bacteria at a temperature and humidity of 28 to 32 ° C. It is aged for 20 to 30 hours at 50 to 55%, and the lactic acid bacteria are selected from the group consisting of Lactobacillales , Streptococcus thermophiles and Bifidobacterium longum . One lactic acid bacterium is used, and the washed oregano raw leaves are heated with steam, It is, but is carried out by heating the washed oregano saengip to 125 to 135 ℃ temperature and from 2 to 10 to 30 minutes of water vapor while at a pressure of 3kgf / cm ^2, drying the oregano saengip heated by said steam, wherein the drying is oregano saengip To dry for 20 to 40 hours at a temperature of 20 to 25 ℃, the dried oregano raw leaves are heated and steamed, but the steaming is put to the heating container after the dried oregano raw leaves 3 to 3 at a temperature of 70 to 80 ℃ The first steamed for 5 minutes, the first steamed oregano raw leaves are proceeded to the second steamed for 20 to 50 seconds at a temperature of 110 to 120 ℃, the steamed with respect to 100 parts by weight of the aged oregano stem Oregano raw leaves are mixed at a weight ratio of 200 to 400 parts by weight, and then fermented for 5 to 10 days at a temperature of 55 to 60 ℃ and a humidity of 55 to 60% oregano To prepare an oregano fermentation extract by preparing a yeast, and mixing the solvent and the ultrasonic wave to the oregano fermentation, the solvent is contained in a weight ratio of 400 to 600 parts by weight based on 100 parts by weight of the oregano mixture, Water is used, the ultrasonic wave using an output of 100 to 200 watts (50 to 100 watts) for 50 to 100 minutes at a vibration frequency of 60 to 80KHz, and remove the oregano stem and oregano raw leaf solids from the oregano fermentation extract, The oregano fermentation extract from which the solids have been removed is centrifuged to separate and separate the lower layer solution in which the fine particles of the oregano stem and the fresh leaf are located, and the supernatant and supernatant positioned above the lower layer solution. Oregano extract is prepared by extracting the oregano fermentation extract located in the supernatant. The oregano extract is first extracted with 70% (v / v) ethanol aqueous solution 10-15 L in an oregano fermented extract at 2000-3000 g for 3-4 hours at 85-90 ° C., followed by 70% (v / v) ethanol aqueous solution. Completing the second extraction at 85 to 90 ° C. for 2 to 3 hours at 4 to 6 l, and preparing an extract until the amount is 1000 to 1500 ml in a vacuum rotary evaporator; And ethanol was added to the extract to 2 to 3 times the weight, and centrifuged at 18,000 to 20,000 rpm for 30 to 40 minutes to remove the polymer fibers and solids that may be included in the oregano fermentation extract, and then the collected supernatant was 1.5 to Prepared by extracting twice with 2.0 times the weight of acetic acid,
After the green tea extract is prepared and washed with green tea leaves, the green tea leaves are dried for 10 to 20 hours in a dryer maintained at a temperature of 23 to 27 ℃ and humidity 40 to 45%, and the dried green tea leaves particle size After grinding to the range of 500 to 3000㎛, the green tea leaves are steamed by heating the ground green tea leaves with steam at a temperature of 110 to 130 ° C. and a pressure of 2.5 to 3.5 kgf / cm ² for 30 to 100 minutes, After mixing the solvent to the steamed green tea leaves, an ultrasonic wave was added to prepare green tea ultrasonic extract, but the mixed liquid was prepared by mixing the solvent in a weight ratio of 300 to 600 parts by weight of the solvent with respect to 100 parts by weight of the steamed green tea leaves. An ultrasonic wave is applied to the ultrasonic wave, and the ultrasonic wave uses an output of 100 to 200 watts for 20 to 40 minutes at a vibration frequency of 60 to 90 kHz, and the solvent has 1 to 4 carbon atoms (C1 to C). One or more solvents selected from alcohols of C4) may be used, and then mixed at a weight ratio of 1 to 3 parts by weight of honey with respect to 100 parts by weight of the green tea ultrasonic extract, and then stored at a temperature of 30 to 35 ° C. for 5 to 10 days. To prepare the green tea extract through the process of concentrating the aged green tea ultrasonic extract, 75% (v / v) ethanol aqueous solution 15ℓ per 2.0kg of the aged green tea ultrasonic extract for 1 hour at 80 to 85 ℃ for 1 hour After the tea extraction, the second extraction was completed under the same conditions with 7 L of the same solvent, and the concentrate was prepared in a vacuum rotary evaporator until 1400 to 1600 ml, diluted with 2 to 3 times the weight by adding water to the concentrate, The solid component was removed by centrifugation at 14,000 to 15,000 rpm for 40 to 60 minutes, and then the collected supernatant was extracted twice with 1.5 times of chloroform. Through the process of removing the layer form, and the aqueous layer extracted three times with ethyl acetate of 1.5 parts by weight to prepare a tea extract,
The baeknyeoncho extract, and washed with purified water dissolved sodium hydrogen carbonate (NaHCO ₃ ) having a concentration range of 1 to 3 (w / w)% and a temperature of 33 to 37 ℃ by collecting baeknyeoncho, The zinnia is separated into zinnia fruit and zinnia stem, and the zinnia fruit is dried in a dryer, the drying of the zinnia fruit is performed for 3 to 7 hours in a dryer maintained at 23 to 27 ℃ and humidity 38 to 42%, The dried zinnia fruit was mixed with purified water and heated to produce the zinnia fruit extract, wherein the zinnia fruit extract was mixed with 500 to 700 parts by weight of purified water with respect to 100 parts by weight of the dried zinnia fruit, and then heated to 90 to 95 ° C. Prepared by heating for 5 to 15 hours at, and drying the baeknyeon stem to remove the moisture contained in the baeknyeon stem, Drying of the stem is carried out for 5 to 10 hours in a dryer maintained at 33 to 37 ℃ and humidity 40 to 45%, mixed with 200 to 400 parts by weight of purified water to 100 parts by weight of the dried centennial stem 60 to 70 ℃ After immersing for 1 to 3 hours at the temperature of the water, the purified water immersed in the baeknyeoncho stem is added to the heater, and the purified water immersed in the baeknyeoncho stem injected into the heater at a temperature of 120 to 140 ℃ and pressure inside the pressure vessel 2-3 kgf / baeknyeon stem extract is prepared by heating for 4 to 8 hours in the range of / cm ² , baeknyeon fruit extract and baeknyeon stem extract by mixing in a weight ratio of 7: 3 to 6: 4 to prepare a baeknyeoncho extract, Effectiveness of selenium produced by the process of aging by storing the baeknyeoncho extract at a temperature of 3 to 7 ℃ for 5 to 10 hours Cosmetic composition for improving scalp health in minutes. delete delete delete

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