KR101988295B1 - Composition for skin moisturizing and anti-wrinkle comprising herbal medicine mixed extract as effective component - Google Patents

Abstract

The present invention relates to a composition for moisturizing skin and alleviating skin wrinkle containing a mixed extract of Glycyrrhiza uralensis, Zizyphus jujube, Pinellia ternate, Zingiber officinale, Bupleurum falcatum, Panax ginseng, and Scutellaria baicalensis as active ingredient. The mixed extract of the present invention has no cytotoxicity, protects cells damaged by ultraviolet irradiation, and remarkably suppresses the expression of elastase. Accordingly, the composition of the present invention can be usefully used in health functional foods for skin moisturization and skin wrinkle alleviation.

Description

Technical Field [0001] The present invention relates to a composition for skin moisturizing and anti-wrinkle containing a herbal medicine mixed extract as an active ingredient,

The present invention relates to a composition for improving skin moisturizing and wrinkles containing an extract of mixed medicinal herbs as an active ingredient.

Skin protects the organs in the body from external stimuli and plays an important role in the maintenance of body homeostasis, such as body temperature control. Such skin is aged by various internal and external factors and is divided into endogenous aging by genetic cause and exogenous aging by environmental cause. Among them, photoaging refers to aging by ultraviolet (UV). Recently, ozone depletion due to environmental pollution has increased the amount of ultraviolet rays, and accordingly, research on photoaging has been attracting attention. In the photoaged skin, appearance characteristics such as roughness, loss of elasticity, wrinkles and irregular pigmentation are observed. Among them, the main research field of photoaging is the change of the skin wrinkles. There have been reported many research results on basic physiological metabolism changes such as synthesis, degradation and moisture content of collagen which is a main constituent of skin in the formation of skin wrinkles by the photoaging. Particularly, ultraviolet rays increase the production of reactive oxygen species, collapse of enzymatic and nonenzymatic antioxidant defenses of skin, decrease collagen decomposition and biosynthesis, and collagen in the dermis layer is remarkably reduced. Collagen degradation enzymes (MMPs) are a major contributor to collagen degradation, which is involved in the degradation of extracellular matrix and basement membrane. Studies have been reported that the enzyme increases activity by ultraviolet rays and inhibits it, thereby increasing skin thickness and wrinkling induced by ultraviolet light. Therefore, it is known that effective control of MMPs is required for prevention and treatment of photoaging.

Meanwhile, licorice ( Glycyrrhiza uralensis Fischer) is known to harmonize the toxicity of all medicines so that the medicinal effect can be seen well, it regulates the fever and morale of the book, makes good communication with all blood vessels, strengthens the muscles and bones, and has detoxification, hepatitis, urticaria, Eczema and so on. It is known to inhibit peptic ulcer, and it has Jinhae, genomic, muscle relaxation, diuretic action, anti-inflammatory action.

The jujube is also called "jujube" or "wood grain". The surface is reddish brown, elliptical, 1.5 ~ 2.5cm long, and has a sweet taste when cooked red. Fruit is not only reproductive, but also dried and dried after being pickled, and is used as sweets, cooking and medicinal products.

In addition, contrary to (Pinellia ternata Breitenbach) is also called perennial herb. Round rootstock is 1㎝ in diameter, with one or two leaves, and three small leaves. The flowers bloom around June and July, and the male flowers are made of anther only without any consideration. The intestines are green and small in size. It is distributed all over Southeast Asia such as China, Japan, and Korea. It is cultivated nationwide in Korea, but Seoul, Suwon, Jeju Island and Ulleungdo Mountain are well known. It has an anti-germ function that calms the respiratory center and removes the spasm of the bronchial tubes to diminish the seawater.

In addition, ginger (Zingiber officinale ) has been used as a food ingredient spice in the home because of its unique flavor and spicy taste, and is widely used for all herbal remedies because of its various activities. The raw materials of herbal medicines are various and the harvested rootstocks are called ginger. The ginger root is boiled or steamed and dried. Ginger secretes digestive juices to promote appetite, promotes gastrointestinal motility, helps digestion and absorption, and also removes feces, flies, stops vomiting, removes wind and swelling, and is used for asthma. In addition, sweating is used in antipyretic drugs, hemostatic disorders, cold winds, etc. Directional sincmi ingredients increase blood circulation and body temperature, and to treat cold and cough by folk remedy, honey is mixed with ginger juice. On the other hand, health is used when the metabolic function is decreased, and it is used as a treatment for cough, dizziness, cold hands and feet, back pain, diarrhea and vomiting. In addition, it is used as a windbreaker and fire extinguishing agent through the planting, and it is used to remove the cold of the five-o'clock oven.

It is also known as Nishi-sho, Nishi-Minari. It grows in the grass and is 40 ~ 70cm high. There are no hairs in the whole aeration, and the branches are divided on the thin long stem. Roots are thick and short. The leaves are staggered, line-shaped or bar-shaped, the edges are flat, and the veins are parallel. The leaf on the root is like petiole with its lower part narrowed, and its length is 10 ~ 30cm. The leaves on the stem are 4 ~ 10cm long, 5 ~ 15mm long and the tip is pointed. It is used as medicines for fever, analgesic, tonic, respiratory, digestive, circulatory diseases.

In addition, ginseng (C. A. Meyer) is commonly grown as a plant growing in deep mountain regions. Its height reaches 60㎝, and rhizomes (roots and roots) are short and stand straight or diagonally, and roots like bellflower develop from the bottom. At the end of the rhizome, one main stem comes out, and at the end, there are three or four leaves, and five long leaves (palm-like compound leaf) hang on the end of the long petiole. It is widely used as sedative, intensive care, correction of the key (胃胃 补 精) · It is widely used as a sedative, it is used as a medicine for depression of the metabolism function by the weakness of gastrointestinal function, ) · Dyspepsia · Vomiting · Chest pain (chest pain) · Relaxing diarrhea (laxative diarrhea) · It is also applied to anorexia.

In addition, gold grows in the grass of the mountain. It is 20 ~ 60cm in height. Several families grow up to give up, have hairs, and branches are divided. Leaves are opposite to each other and have a straight edge. The flowers bloom in July-August, the moonlight turns and runs on the side of the gun inflorescence. Corolla (corolla) is curved at the bottom, straight, tubular, 1.5-2.5cm long, lips-shaped. Calyx is bell-shaped. Root is used as fever, diuretic, branch, insect, and anti-inflammatory.

MMP-1 inhibitors including gold extracts have been disclosed in Japanese Laid-Open Patent Application No. 2013-203679 as prior arts relating to the prevention or improvement of the licorice, jujube, antidote, ginger, Open Patent Publication No. 2006-0027764 discloses a composition containing the extract of Shiho as an active ingredient, but no prior art relating to a composition for skin moisturizing and wrinkle improvement containing the mixed extract of the herbal medicines of the present invention as an active ingredient has been disclosed.

The present invention provides a composition for improving skin moisturizing and wrinkles containing an extract of mixed medicinal herbs as an active ingredient, and the present invention provides a composition for improving skin moisturizing and wrinkles, Protects the cells from damage by ultraviolet irradiation, has a remarkable activity of inhibiting elastase, restores the reduced epidermal thickness by UV irradiation, and restores the hyaluronic acid content reduced by UV irradiation to almost normal level By confirming, the present invention has been completed.

In order to achieve the above object, the present invention provides a health functional food composition for improving skin moisturization and skin wrinkles containing, as an active ingredient, a mixed extract of licorice, jujube, bamboo, ginger, Seiho, ginseng and gold.

In addition, the present invention provides a herbal composition composition for skin moisturization and skin wrinkle improvement, which comprises, as an active ingredient, a mixed extract of licorice, jujube, bamboo, ginger, citron, ginseng and gold.

The present invention relates to a composition for improving skin moisturizing and skin wrinkles containing an extract of mixed medicinal materials as an active ingredient. The mixed extract of the present invention protects cells damaged by ultraviolet irradiation and remarkably suppresses the expression of elastase will be. Accordingly, the composition of the present invention can be usefully used in health functional foods for skin moisturization and skin wrinkle improvement.

1 is a graph showing the cell survival rate (%) of the mixed extract of Licorice, Jujube, Banha, Ginger, Seok, Ginseng and Golden of the present invention and the herbal extract alone The results of MTS assay after treatment of samples with concentration (10, 50, 100 and 200 μg / ml).
FIG. 2 is a graph showing the effect of UVB irradiation on the cytotoxic effect of the extracts of licorice, jujube, antimicrobial, ginger, citrus, ginseng and gold of the present invention and the herbal extract alone (comparison group) (UV cross-linker, UVP) for 30 min, and then subjected to MTS assay for 24 hrs. Each sample was treated with concentration (50 ㎍ / ㎖ and 100 ㎍ / ㎖) .
FIG. 3 is a graph showing the elastase inhibitory activity of the mixed extract of Licorice, Jujube, Banha, Ginger, Seiho, Ginseng and Golden of the present invention and the herbal extract alone (Comparative group). **** indicates that the inhibitory activity of elastase inhibition (%) in the group treated with the mixed extract of the present invention was statistically significantly increased as compared with that of the herbal extract alone, p < 0.0001.
FIG. 4 is a graph showing changes in the content of hyaluronic acid according to the treatment of the mixed extract of Licorice, Jujube, Banha, Ginger, Seo, Ginseng and Golden of the present invention and the single extract of the herbal medicine as a comparative group. #### means that the hyaluronic acid content of the ultraviolet irradiation group (UVB) was statistically significantly decreased compared to the control without any treatment, which means that p <0.0001. Means that the hyaluronic acid content of the experimental group treated with the mixed extract of the present invention and irradiated with ultraviolet light was significantly increased compared to the ultraviolet irradiation group, which means that p <0.0001. ** and **** indicate that the content of the mixed extract of the present invention was statistically significantly increased compared to that of the herbal extract alone, ** means p <0.01 and **** means p <0.0001.
FIG. 5 shows the results of confirming the decrease of the epidermal thickness and the increase of the collagen by administration of the mixed extract of Licorice, Jujube, Banha, Ginger, Seishin, Ginseng and Gold according to the present invention. A is the result of H & E staining, B is the result of Masson's trichome staining, and C is the skin thickness. # Indicates that the epidermal thickness of ultraviolet irradiated group was statistically significantly increased compared to the normal group, and p <0.05. * Indicates that the skin thickness of the group treated with the mixed extract of the present invention was statistically significantly lower than that of the ultraviolet irradiation group, which is p < 0.05.
FIG. 6 shows the result of confirming that the content of hyaluronic acid decreased by UV irradiation was increased by administration of the mixed extract of the present invention. # Indicates that the hyaluronic acid content in the UV irradiated group was statistically significantly decreased compared with that in the normal irradiated group and that p < 0.05 means that the hyaluronic acid in the group irradiated with the mixed extract of the present invention , And that the content of lonic acid increased statistically significantly, p <0.05.

The present invention relates to a health functional food composition for skin moisturizing and skin wrinkle improvement, which comprises, as an active ingredient, a mixed extract of licorice, jujube, bamboo, ginger, citron, ginseng and gold.

The mixed extract of licorice, jujube, ganoderma, ginger, ginseng, and gold is referred to as "Soshifotang" as a prescription for Oriental medicine.

The mixed extract of licorice, jujube, ganoderma, ginger, ginseng, ginseng and gold may be prepared by a method including, but not limited to, the following steps:

(1) extracting a mixture of licorice, jujube, ganoderma, ginger, ginseng, ginseng and gold by adding an extraction solvent;

(2) filtering the extract of step (1); And

(3) The step of extracting the filtered extract of step (2) by concentration under reduced pressure and drying.

In step (1), the extraction solvent is preferably selected from water, a C ₁ -C ₄ lower alcohol or a mixture thereof, more preferably ethanol, even more preferably 70% (v / v) ethanol But is not limited thereto.

In the above production method, any conventional method known in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction may be used. Preferably, the extraction solvent is added by 1 to 20 times, preferably 5 to 15 times, the weight of the mixture of dried licorice, jujube, bamboo, ginger, citron, ginseng and gold. The extraction temperature is preferably 4 to 50 ° C, but is not limited thereto. The extraction time is preferably 0.5 to 10 hours, more preferably 0.5 to 5 hours, most preferably 1 hour, but not always limited thereto. In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in the step (3), but it is not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.

The mixture extracts of 18 to 26% by weight, 8 to 12% by weight of ginseng, 5 to 7% by weight of licorice, 9 to 14% by weight of jujube, 31 To about 46% by weight of gold, from about 8% to about 13% by weight of undiluted and from about 0.8% to about 1.2% by weight of ginger.

The mixed extracts of licorice, jujube, bamboo, ginger, citrus, ginseng and gold are characterized by having an excellent inhibitory activity against elastase. Particularly, when the extract of herbal medicines contained in the mixed extract alone is used alone, And the activity of the inhibition of the enzyme activity was statistically significantly increased.

The composition may be prepared in any form of powder, granule, ring, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably used depending on the purpose of use (prevention or improvement). Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in the case of long-term intake intended for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount of more than the above range.

There is no particular limitation on the kind of the health functional food. Examples of foods to which the health functional food composition can be added include meat products, sausages, breads, chocolate, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, dairy products including ice cream, various soups, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when it is made of a drink, it may contain, in addition to the active ingredient, a natural carbohydrate or a flavoring agent as an additional ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, For example, xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected in the range of 0.01 to 0.1 part by weight based on 100 parts by weight of the health functional food composition of the present invention.

The present invention also relates to a herbal composition composition for improving skin moisturization and skin wrinkles containing, as an active ingredient, a mixed extract of licorice, jujube, bamboo, ginger, citron, ginseng and gold.

The medicinal herb composition of the present invention is meant to be produced by the oriental medicine, but is not limited thereto. In addition, a portion of the herbal medicine extract constituting the mixed extract may be excluded or arbitrarily added by the prescription of the oriental medicine doctor.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example  1. Licorice, jujube, Inferiority , Ginger, Seoho, ginseng, and gold

Water was added to the total weight of each of licorice, jujube, ganoderma, ginger, Seoho, ginseng and golden, and the mixture was refluxed and cooled for 2 hours for 3 hours. The filtrate was filtered using a vacuum evaporator to remove the solvent , And 0.64 g of Seiho, 0.3 g of ginseng, 0.17 g of licorice, 0.34 g of jujube, 1.13 g of gold, 0.31 g of undiluted and 0.03 g of ginger were mixed to prepare the same extract The mixed extract of the present invention was prepared.

Example  2. Cell survival analysis

HaCaT cells were used for the analysis of cell viability and HaCaT cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin , Gibco) at 37 ° C and 5% CO ₂ .

The HaCaT cells were diluted with the concentrations of 10, 50, 100 and 200 μg / ml of the extracts, ginseng, licorice, jujube, (CellTiter Aqueous One Solution Cell proliferation assay kit, 3- (4,5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4- sulfophynyl) -2H- tetrazolium, inner salt MTS, Promega Co. Madison, WI, USA), the cell viability was measured at 490 nm with a microplate reader (Molecular Devices, Sunnyvale, Calif., USA).

As a result, the relative cell viability (%) when the sample of the present invention was treated was slightly increased or maintained as shown in Fig. Therefore, it was found that the mixed extract of the present invention had little cytotoxicity.

Example  3. Confirmation of cytoprotective effect by ultraviolet irradiation

Each sample was diluted by HaCaT cells for 24 hours, irradiated with ultraviolet light (UV Crosslinker, UVP) for 30 minutes, and then subjected to MTS assay (CellTiter Aqueous One Solution Cell proliferation assay kit, 3- (4,5-dimethylthiazol 2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophynyl) -2H-tetrazolium, inner salt; MTS, Promega Co. Madison, Wis., USA) (Molecular Devices, Sunnyvale, Calif., USA).

As a result, as shown in Fig. 2, it was confirmed that the cell survival rate (%) was reduced by about 50% by UVB irradiation, and the cell survival rate (%) was increased when the mixed extract of the present invention was treated after UV irradiation , It was judged that the mixed extract of the present invention had a cytoprotective effect.

Example  4. Confirm Elastase Inhibitory Activity

In order to confirm the elastase inhibitory activity of each sample extract of the present invention and each herbal medicine constituting the sample, 140 μl of Tris buffer solution (0.2 M Tris buffer, pH 8.0) and 20 μl of each sample of 1 mg / ml And 20 μl of 5mM N-Succinyl-Ala-Ala-p-nitroanilide (Sigma S4760) were added to the substrate solution. 20 μl of 10 μg / ml of elastase (Sigma, E7885) , The amount of p-nitroaniline produced at 410 nm was measured with a microplate reader. Ascorbic acid was used as a positive control.

As a result, as shown in Fig. 3, not only the mixed extract of the present invention, but also the herbal medicine extracts alone showed an elastase inhibitory activity. In particular, it was confirmed that the elastase inhibitory activity of the mixed extract was statistically significantly increased compared with the herbal medicine extract alone.

Example  5. Analysis of expression of hyaluronic acid

HaCaT cells were diluted with each concentration for 24 hours, irradiated with ultraviolet light (UV Crosslinker, UVP) for 30 minutes, and the supernatants of each experimental group were collected and incubated in a hyaluronic acid ELISA kit (R & D Systems, Inc., Minneapolis, , USA) was used to measure expression of hyaluronic acid expressed in cells. The reaction product was subjected to an absorbance assay to measure the amount of hyaluronic acid expressed at 450 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA).

As a result, as shown in FIG. 4, the content of hyaluronic acid was decreased by ultraviolet irradiation, and the hyaluronic acid content was increased in all the groups treated with the herbal medicine extract alone as well as the mixed extract of the present invention. The increase in the hyaluronic acid content of the mixed extracts was statistically significantly higher than that of the herbal medicine extracts alone.

Example  6. Histological analysis of skin

In order to analyze the histological changes of the skin, the mixed extract of the present invention was administered to the hairless mouse group once a day in an amount of 100 mg / kg, five times a week, and three times a week Ultraviolet light was irradiated for a total of 12 weeks.

Thereafter, in order to confirm the wrinkle-suppressing effect, the skin of the animal group to which no treatment was performed, the normal group, the ultraviolet irradiation group (the group irradiated with ultraviolet rays three times a week for 12 weeks), the ultraviolet irradiation, Tissue was removed and tissue staining was performed.

The skin of each experimental group was extracted and fixed in 10% neutral formalin solution. After washing, dewatering, transparent and permeation process, it was embedded with paraffin and cut into 4 μm thick sections. The sections were stained with H & E (Hematoxylin & Eosin) staining and Masson's trichome staining Respectively. The thickness from the keratin layer to the epidermal basement membrane of H & E stained tissue was measured using a microscope.

As shown in the results of the H & E staining, it was confirmed that the stratum corneum layer and the skin layer thickness were increased compared to the control group not treated with ultraviolet light, and the skin layer was relaxed and the skin thickness decreased (Fig. 5A). The skin thickness from the keratin layer to the epidermal basement membrane of the H & E-stained tissue was measured using a microscope. As a result, it was confirmed that the epidermal thickness was increased in the ultraviolet irradiation group, (Fig. 5C). In addition, as shown in the result of Masson's trichome staining, the stratum corneum was thickened in the group irradiated with ultraviolet rays, and no collagen fiber was observed. However, it was confirmed that the collagen fibers were increased in the mixed extract-administered group as compared with the ultraviolet-irradiated group (FIG. 5B).

Example  7. Analysis of content of hyaluronic acid

Hyaluronic acid was extracted from the skin tissue of each experimental group and examined by hyaluronic acid ELISA (Hyaluronic Acid) for the purpose of confirming the change of the content of hyaluronic acid, which serves to prevent skin moisturizing function and skin barrier damage, kit (R & D Systems, Inc., Minneapolis, MN, USA) was used to measure the content of hyaluronic acid. The reaction product was subjected to an absorbance assay to quantitatively analyze the content of hyaluronic acid.

As a result, it was confirmed that the content of hyaluronic acid decreased in the vehicle irradiated with ultraviolet rays, and in the group treated with the mixed extract of the present invention, the content of hyaluronic acid was increased and the moisturizing function damaged by ultraviolet irradiation was increased (Fig. 6).

Claims (6)

8 to 12% by weight of ginseng, 5 to 7% by weight of licorice, 9 to 14% by weight of jujube, 31 to 46% by weight of gold, 8 to 13% A health functional food composition for improving skin moisturization and skin wrinkles containing 1.2% by weight of a mixed extract of ginger as an active ingredient. [Claim 2] The composition according to claim 1, wherein the extraction solvent of the mixed extract is water, a C ₁ -C ₄ lower alcohol or a mixture thereof. delete The composition according to claim 1, wherein the composition inhibits expression of elastase. The composition according to claim 1, wherein the composition is prepared from one of powder, granule, ring, tablet, capsule, candy, syrup and beverage. 8 to 12% by weight of ginseng, 5 to 7% by weight of licorice, 9 to 14% by weight of jujube, 31 to 46% by weight of gold, 8 to 13% A herbal composition composition for improving skin moisturization and skin wrinkles containing 1.2 wt% mixed extract of ginger as an active ingredient.

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