KR102276213B1 - Cosmetic composition for hair loss care applied to the natural plant composite material extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for hair loss care to which an extract of a natural plant composite material is applied. Based on 100 parts by weight of deionized water, 1.2- hexanediol 2.00 parts by weight; 1.00 parts by weight of panthenol; Produce-400 0.50 to 5.00 parts by weight; 0.30 to 2.00 parts by weight of niacinamide; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.06 to 0.10 parts by weight of biotin; 0.03 parts by weight of disodium EDTA; 0.03 to 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 to 0.30 parts by weight of pyridoxine HCl; Ammonium acryloyldimethyltaurate (Ammonium Acryloyldimethyltaurate VP copolymer) 0.05 to 0.10 parts by weight; 1.00 parts by weight of L-arginine; 0.50 parts by weight of citric acid; 0.60 parts by weight of sodium citrate dihydrate; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 10.00 parts by weight of butylene glycol; 0.10 parts by weight of olive oil; 0.03 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 to 0.15 parts by weight of salicylic acid; 0.60 to 0.80 parts by weight of PEG-60 (hydrogenated castor oil); Lemon essential oil 0.08 parts by weight; 1.60 to 5.00 parts by weight of natural plant composite extract; includes
According to the present invention, there is a beneficial effect on hair loss prevention and scalp health by compounding a natural plant composite material extract and medicinal ingredients that have been verified for antioxidant, anti-inflammatory, and antiseptic effects.

Description

Cosmetic composition for hair loss care applied to the natural plant composite material extract

The present invention relates to a cosmetic composition for hair loss care to which a natural plant composite material extract is applied, and more particularly, it prevents hair loss and It relates to a cosmetic composition for hair loss care to which an extract of a natural plant composite material beneficial to scalp health is applied.

A characteristic of modern society is a society that values beauty such as competitive appearance, and the resulting shampoo, dye, and perm are unavoidable social behaviors such as selective beauty care. In addition, stress applied in a constantly competitive society, and individual genetic characteristics Hair loss on the back is a physiological phenomenon that no one wants.

There are about 100,000 hairs, and in general, about 40 to 100 hairs fall out of human hair per day. In addition, it has the characteristic of regenerating the hair as much as the amount that fell off.

However, in the case of internal and external abnormalities of the scalp and human body, the number of hairs that are lost per day increases to about 200, and the existing hair growth period is shortened and the hair vellus development phenomenon can be seen.

The cause of hair loss is mainly due to the scalp type, and the oily scalp does not have good blood circulation, which weakens the hair roots and hair follicles, which prevents the generation of new hair.

In particular, in the case of men, the hair root becomes thinner by male hormones and the secretion of sebum is activated. Failure to keep the scalp clean can cause hair loss.

In addition, dry scalp hair loss can result in hair loss due to weakening of the hair roots because the scalp is pulled as opposed to oily scalp.

In addition to hair loss due to stress, when stress is continuously accumulated, it causes blood circulation disorders and insomnia, and the resulting scalp tension changes the scalp to oily, which adversely affects hair growth and development as well as hair growth. will give

In addition, hair loss caused by eating habits leads to the frequent consumption of oily and savory foods containing a lot of fat as our diet is westernized. These foods have the same effect as male hormones, turning the scalp into oily and causing hair loss.

In addition, when drinking alcohol as a result of hair loss due to tobacco, alcohol components promote inflammation and sebum secretion, and smoking lowers body temperature and exports blood vessels, impeding blood circulation and causing a lack of vitamins. Since it causes hair loss and adversely affects the growth and development of hair, it is necessary to prevent hair loss through moderate drinking, smoking cessation, and vitamin intake.

In addition, as for hair loss due to ultraviolet rays, strong ultraviolet rays directly stimulate the scalp to dry the hair roots and increase inflammation to promote hair loss. Also, the ultraviolet rays destroy the keratin, the protein layer of the hair, and damage the hair, thereby making the hair thinner. This eventually leads to hair loss.

Therefore, research and technology development for preventing hair loss and promoting hair growth are being actively conducted, but research results or technology development that produce a distinct effect are still insufficient.

Registered Patent Publication No. 10-0659490

In order to solve the conventional disadvantages as described above, the present invention provides a natural plant composite extract for preventing hair loss and for scalp health by composing a natural plant composite extract that has proven excellent antioxidant, anti-inflammatory, and antiseptic effects and medicinal ingredients. An object of the present invention is to provide a cosmetic composition for hair loss care applied.

The cosmetic composition for hair loss care to which the natural plant composite material extract of the present invention is applied in order to achieve the object as described above,

With respect to 100 parts by weight of deionized water,

1.2- hexanediol 2.00 parts by weight; 1.00 parts by weight of panthenol; Produce-400 0.50 to 5.00 parts by weight; 0.30 to 2.00 parts by weight of niacinamide; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.06 to 0.10 parts by weight of biotin; 0.03 parts by weight of disodium EDTA; 0.03 to 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 to 0.30 parts by weight of pyridoxine HCl; 0.05 to 0.10 parts by weight of Ammonium Acryloyldimethyltaurate VP copolymer; 1.00 parts by weight of L-arginine; 0.50 parts by weight of citric acid; 0.60 parts by weight of sodium citrate dihydrate; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 10.00 parts by weight of butylene glycol; 0.10 parts by weight of olive oil; 0.03 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 to 0.15 parts by weight of salicylic acid; 0.60 to 0.80 parts by weight of PEG-60 (hydrogenated castor oil); Lemon essential oil 0.08 parts by weight; 1.60 to 5.00 parts by weight of natural plant composite extract; includes

In one embodiment, the present invention

With respect to 100 parts by weight of deionized water,

Disodium Laureth Sulfosuccinate 10.00 to 40.00 parts by weight; 5.00 to 25.00 parts by weight of lauryl glucoside; 5.00 to 10.00 parts by weight of sodium cocoyl apple amino acid; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 4.00 to 10.00 parts by weight of glycerin; 4.00 to 5.00 parts by weight of sorbitol; Decylglucoside 5.00 parts by weight; Polyquaternium-67 0.10 to 1.00 parts by weight; Polyquaternium-10 0.10 to 0.26 parts by weight; 0.20 to 0.24 parts by weight of guarhydroxypropyltriammonium chloride; 0.30 to 2.00 parts by weight of niacinamide; 0.06 to 0.10 parts by weight of biotin; Produce-400 0.50 to 3.00 parts by weight; 0.03 parts by weight of disodium EDTA; 3.00 to 10 parts by weight of sodium lauroyl glutamate; 2.00 to 3.00 parts by weight of glucamate; 5.00 parts by weight of cocamidopropyl betaine; 1.2-hexanediol 2.00 parts by weight; 0.50 parts by weight of moringa oil: 0.30 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 parts by weight of olive oil; 3.00 parts by weight of liquid silk amino acid; 1.60 to 3.00 parts by weight of natural plant composite material extract; It is characterized in that it includes.

In one embodiment, the present invention

With respect to 100 parts by weight of deionized water,

5.00 parts by weight of sorbitol; 1.2-hexanediol 2.00 parts by weight; 0.50 to 2.00 parts by weight of niacinamide; PCA-Na (Na-pyrolidonecarboxylate) 5.00 to 10.00 parts by weight; Produce-400 3.00 parts by weight; 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 parts by weight of adenosine; Biotin 0.05 to 0.10 parts by weight; 0.01 parts by weight of lactobionic acid; 0.05 parts by weight of gluconolactone; 5.00 parts by weight of ethanol; 0.05 parts by weight of hinokitiol oil; 0.15 parts by weight of menthol; 0.05 parts by weight of salicylic acid; 0.01 parts by weight of mandelic acid; 0.25 to 0.30 parts by weight of lemon essential oil; 0.70 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tocopheryl acetate; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 3.00 to 5.00 parts by weight of a natural plant composite material extract; It is characterized in that it includes.

The natural plant composite material extract,

Mistletoe, fig, Geumhwagyu, wheat sprout, Yeongyo, red peony, white mountain tea, and green tea are characterized in that it contains any one or more.

The natural plant composite material extract,

5% by weight mistletoe; figs 5% by weight; 25% by weight of Keumhwa Beef; wheat germ 5% by weight; 15% by weight per year; About 15% by weight of red peony; Baeksan tea 5% by weight; Green tea 20 to 25% by weight; It is characterized in that it includes.

According to the present invention, complex with natural plant composite material extracts (fig, mistletoe, Geumhwagyu, wheatgrass, Yeongyo, red peony, baeksan tea, green tea) with excellent antioxidant, anti-inflammatory, and antiseptic effects and pharmaceutical ingredients such as peptides, panthenol, biotin, etc. By constructing the composition, it is possible to manufacture a cosmetic composition for hair loss care, thereby maximizing the skin penetration effect around the hair follicles and hair follicles of the scalp, thereby inducing the promotion of hair in the resting state to the hair in the growth phase, as well as preventing hair loss on the scalp And it has the effect of maximizing hair growth.

In addition, according to the present invention, there is also an effect of sedative action to prevent the stimulation as stress repeatedly applied to hair and scalp, such as dyeing and perm, and the resulting inflammation, dandruff, or hair loss.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art to which the present invention pertains can easily implement them. However, since the description of the present invention is merely an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiment described in the text. That is, since the embodiment may have various changes and may have various forms, it should be understood that the scope of the present invention includes equivalents capable of realizing the technical idea. In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all of them or only such effects, it should not be understood that the scope of the present invention is limited thereby.

The meaning of the terms described in the present invention should be understood as follows.

Terms such as “first” and “second” are for distinguishing one component from other components, and the scope of rights should not be limited by these terms. For example, a first component may be termed a second component, and similarly, a second component may also be termed a first component. When a component is referred to as being “connected” to another component, it may be directly connected to the other component, but it should be understood that other components may exist in between. On the other hand, when it is mentioned that a certain element is "directly connected" to another element, it should be understood that the other element does not exist in the middle. On the other hand, other expressions describing the relationship between elements, that is, "between" and "immediately between" or "neighboring to" and "directly adjacent to", etc., should be interpreted similarly.

The singular expression is to be understood as including the plural expression unless the context clearly dictates otherwise, and terms such as "comprises" or "have" refer to the described feature, number, step, action, component, part or these It is intended to indicate that a combination exists, and it should be understood that it does not preclude the possibility of the existence or addition of one or more other features or numbers, steps, operations, components, parts, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Terms defined in general used in the dictionary should be interpreted as being consistent with the meaning in the context of the related art, and cannot be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present invention.

The cosmetic composition for hair loss care to which the natural plant composite material extract of the present invention is applied includes a natural plant composite material extract to prevent hair loss and benefit scalp health.

The natural plant composite material extract includes mistletoe, fig, Geumhwagyu, wheat sprout, Yeongyo, red peony, Baeksan tea, and green tea.

Hereinafter, the present invention will be specifically described.

(1) mistletoe

Mistletoe (Viscum Album) is a semi-parasitic plant (hemipatasote) that has dark green leaves and stems, and branches are continuously divided. In Korea, it is parasitic on oak, hackberry, mulberry, cherry, and birch trees.

Flowers are yellow in March, bloomed at the tip of branches, without stalks, and small bracts are plate-shaped and male and female. The inflorescence is bell-shaped and has 4 branches, and the fruit is round and ripens in light yellow in October.

It has been reported that the components of mistletoe include lectin, amyrin, inositol, viscotoxin, viscine, flavonoid, polysaccharide, tyramine, histamine, and choline.

These components are known to have pharmacological effects such as cholesterol lowering, blood pressure lowering action, diuretic, antibacterial and antiviral effect, back pain, postpartum, frostbite.

In particular, lectin, the main component of mistletoe, has been reported to have a cytotoxic effect, an immune enhancing effect, and anticancer activity.

Main ingredients: lectin, flavonoid, histamine

(2) figs

Fig (Ficus Carica) is the fruit of the fig tree, usually round or conical in shape, and has various colors such as green, brown, and black. It has a strong sweet taste, so it is eaten raw, dried, or processed and used as a cooking ingredient.

As an alkaline food, in ancient Egypt, Rome, and Israel, it was also used as a tonic or as a medicine to treat cancer and liver disease. In the private sector, it is used as medicine for indigestion, constipation, diarrhea, hemoptysis, neuralgia, skin diseases, anemia, and gynecological diseases, while raw juice is used to treat hemorrhoids and warts in main production areas.

In Korea, more than 90% of the total domestic production is produced in Yeongam-gun, Jeollanam-do. The shape of the fruit is round, flat and round, and cone-shaped. The color of the skin of the fruit varies, including green, yellowish green, yellow, reddish green, purplish brown, and purplish black.

In Europe and the United States, it is widely used as dried confection, and in Korea and Japan, it is mainly eaten raw. Varieties are divided into four lineages: Carpri, Smyruna, Sanpedro, and Common, and are mainly cultivated in Europe and America.

In Korea, the normal type and the San Pedro type are grown a lot, and the main ingredients are about 10% of sugar (glucose and fructose), so it has a strong sweetness. Organic acids include malic acid and citric acid, as well as benzaldehyde, which is effective in treating cancer, and ficin, a proteolytic enzyme.

In addition, it is rich in enzymes such as lipase, amylase, and oxidase, as well as fiber and protein.

Main ingredients: benzaldehyde, ficin,

(3) Geumhwagyu

Geumhwagyu (Hibiseu Smanihot L.) As an annual herbaceous plant, both roots, leaves, and flowers can be used medicinally. Flowers bloom in July-August, also called gold hibiscus, or golden sunflower in China.

Keumhwa Beef has a sweet taste, non-toxic, and contains the flavonoid oleic acid, vitamin E, selenium, and selenium. It also contains linoleic acid, which relieves constipation and toxins in the intestine, prevents and improves gastric ulcer, gastritis and arteriosclerosis, reduces the occurrence of heart disease, and purifies the blood.

Collagen is a component that functions as the main protein of the skin and is known to be effective in maintaining moisture and elasticity of the skin.

Hypeoside, which has strong anti-inflammatory action, contains a large amount of essential amino acids, and natural phytoestrogens are good for menopausal syndrome.

It is said that the ginseng stalk has the effect of increasing sleep mass, stabilizing the mind and body, lowering blood pressure, enhancing yang, rejuvenating the kidneys, and enhancing immunity. Flowers have antipyretic detoxification, bowel movement, anti-inflammatory and analgesic effects, and have the effect of relieving fatigue and lowering blood pressure.

Main Ingredients: Flavonoid, Vitamin E, Linoleic acid, Selenium

(4) wheat sprout

Wheat sprout (Triticum Aestivum L.) is a vegetable grown by sprouting wheat seeds.

It grows by laying soil on the bed, planting soaked whole wheat, and then watering it just like when growing bean sprouts. Before germination, place it in a shaded place, and when germination occurs, move it to a sunny and well-ventilated place. After a week or so, when the buds have grown about 15 cm, cut them and use them.

When harvesting, the beak is cut and the stem is cut, and after cutting, the shoot grows again, so it can be eaten several times.

Wheatgrass purifies the blood, cleanses the intestines, promotes appetite and helps in weight loss, and promotes growth in children.

In addition, it has an excellent effect on relieving insomnia, anemia and constipation, and increases immunity by increasing white blood cells in the blood sugar control heart, lungs, intestines, and blood vessels. In addition, it detoxifies the liver and produces red blood cells through chlorophyll, and it is also effective in detoxifying heavy metals.

Main Ingredients: Amino acid, Biotin, Vitamin A, Vitamin B12, Vitamin C

(5) Yeongyo

Yeongyo (Forsythiae Fructus) is a plant of the ash family, a deciduous tree with yellow flowers, also called forsythia. It is a common endemic species in the Korean peninsula, and it can be found all over the country except in Hamgyeong-do, near houses. It is also planted in a pile next to a fence or road, or planted in one or two groups. It also grows wild at the foot of a sunny mountain.

Many stems are clustered together and the branches are divided and grow densely. The stem is empty. It is a separate male and female tree, and flowers bloom before the leaves in early spring, and 1~3 yellow flowers hang in the leaf axils.

The leaves are opposite, long oval, pointed at the tip, and have serrations or flattening on the upper part. Both front and back are hairless, the front is shiny and the back is white.

The fruits ripen in egg shape in autumn with a pointed tip and ripen to brown. The fruit of forsythia is called Yeongyo, and it is harvested and used as medicine. Usage: It is prescribed for cold, fever, purulent disease, lymphadenitis, urinary incontinence, boil, nephritis, eczema, etc.

Main Ingredients: Flavonoid, Saponin, Alkaloid, Oleanolic acid

(6) Red Peony

Red peony (Paeonia Lactiflora Pall) means that the small roots of the red peony are red and the small hamburg flowers are bright and beautiful in full bloom. Its root is called peony because it is a drug that cures diseases well. It has the effect of lowering the heat in the body, purifying the blood, and alleviating pain by removing the blood stasis.

It is a local product produced in Gangwon-do. It is usually collected in February and August of the lunar calendar and dried in the sun for use. It relieves symptoms such as abdominal pain, hard lumps in the stomach, fever with chills, and hard and painful lower abdomen. It relieves pain, promotes urination, protects qi, promotes blood circulation, and evens the stomach.

In addition, it not only improves water metabolism, but also has the effect of improving the function of the bladder and large intestine. It is also used to treat wind sickness and weak symptoms. It was used for various diseases occurring in women and various symptoms before and after childbirth. Red peony makes urine flow well and lowers qi.

Main Ingredients: Flavonoid, Tanin, Triterpenoid

(7) Baeksan Tea

White mountain tea (Ledum Palutre) grows under the forests of high mountains.

It is 15-70cm in height, young shoots sprout from the root, and dark brown hairs are densely formed on young branches. The leaves are alternate phyllotaxis, long oval or lanceolate, 2-7cm long, 4-12mm wide, dark green, and wrinkled. On the back side, brown and white hairs are dense, fragrant, and the edges curl backwards.

The petiole is 3-4 mm long. White flowers bloom in May-June, 7-10mm in diameter, and hang on corymbal inflorescences. Ears of flowers have branches and hairs. The bract is egg-shaped, and there are 5 sepals and 5 petals, 10 stamens, and 1 pistil. The fruit is a capsule, a long oval shape, and ripens in September.

The leaves are 1-2 cm long and 2-3 mm wide, and those with no white hair on the back are called narrow baeksan tea. Baeksan tea has the effect of regulating menstruation, so it treats menstrual irregularities, infertility, and gastric ulcers. In Europe, it is also used for rheumatoid arthritis and joint pain.

Main Ingredients: Tanin, Terpene

(8) green tea

Green tea (Camellia Sinensis) is a tea made from unfermented tea leaves. Green tea was first produced and used in China and India. After that, it spread to various parts of Asia such as Japan, Ceylon, Java, and Sumatra.

Tea is divided into green tea, black tea, and oolong tea depending on whether it is fermented during the manufacturing process. Young leaves from newly sprouted branches are used to make tea, and the leaves are usually picked three times in May, July, and August. The best tea is picked in May.

The tea tree is an evergreen tree that grows well in areas with relatively warm and heavy rainfall. In order to make green tea, the leaves are immediately heated to destroy the oxidase enzymes to maintain their green color, while at the same time, the moisture is evaporated to dry the leaves in a soft and easy-to-roll condition. In the old days, people rubbed and dried the leaves by hand in a cauldron. After that, heating is continued to remove most of the moisture, making it somewhat crispy.

In recent years, tea is manufactured using organic, organic, oil-based, reconstructing, refinery, and dryer machines. The polyphenol component of green tea has anticancer action by reducing the occurrence of cancer, and it prevents cardiovascular disease by reducing cholesterol level. It is also effective in diabetes and is effective in preventing skin damage and whitening the skin.

Main Ingredients: Cianidanol, Amino acid, Vitamin B1, Vitamin B2, Vitamin C, Caffeine

<Experiment 1> Antioxidant activity experiment of each natural plant material

(A) Experimental result of antioxidant activity of 70% ethanol extract of each of 8 raw materials

In the case of yeonkyo, red peony, baeksan tea, and green tea 70% ethanol extract, both DPPH electron donating ability and ABTS+ radical scavenging activity showed higher or similar activity than vitamin C at a low concentration of 50 μg/ml, indicating that they had excellent antioxidant effects.

In the case of figs, wheat sprouts, mistletoe, and 70% ethanol extracts of Geumhwagyu, even at a high concentration of 1,000 μg/ml, both DPPH electron donating ability and ABTS+ radical scavenging activity showed lower effects than Vitamin C, but they had anti-inflammatory and hair loss prevention effects. known from the investigation.

(1) DPPH electron-donating ability test results (Yeonkyo, red peony, Baeksan tea, green tea)

(2) DPPH electron donating ability test results (figs, wheat sprouts, mistletoe, goldfinch)

(3) ABTS+ Radical scavenging activity test result (Yeonkyo, Red peony, Baeksan tea, green tea)

(4) ABTS+ Radical scavenging activity measurement test results (fig, wheat sprout, mistletoe, goldfish)

<Experiment 2> Optimal complex experiment of natural plant materials

Figs, mistletoe, Geumhwagyu, wheatgrass, yeonkyo, red peony, white mountain tea, and green tea have excellent antioxidant, anti-inflammatory, and wrinkle-improving effects in common. The wrinkle effect was confirmed through previous studies.

Based on the analysis of these results, considering the efficacy of the eight natural plant materials constituting the natural plant composite material extract, it is composed of CHC-1 ~ CHC-5 and confirmed the efficacy effect as a complex hair product showing the optimal effect. Possibility of application as an active ingredient for the

Main effect CHC-1 CHC-2 CHC-3 CHC-4 CHC-5 FIG anti-inflammatory, antibacterial 15 10 5 5 5 mistletoe anti-inflammatory, antipyretic 15 10 5 5 5 Geumhwagyu antioxidant, anti-inflammatory 15 10 15 5 25 wheat germ anti-inflammatory, anti-cancer 15 10 5 5 5 annual school antioxidant, anti-inflammatory 10 15 20 25 15 red peony antioxidant, antipyretic 10 15 20 25 15 Baeksan Tea antioxidant, anti-inflammatory 10 15 10 2 5 green tea antioxidant, anti-inflammatory 10 15 20 25 25 total amount 100 100 100 100 100

(A) Sample extraction

Extraction of the sample was repeated three times by adding 70% ethanol 10 times the weight of the sample and immersing it at room temperature for 24 hours. After filtration and concentration, each extract was freeze-dried and stored in a refrigerator to be used as a sample for this experiment.

(B) Extraction yield

According to the above-described extraction method using 70% ethanol, 8 kinds of natural plant materials were measured and extracted for each composition as shown in Table 2, and the results of measuring the yield are shown in Table 3.

For each composition, CHC-1: 20.8%, CHC-2: 22.4%, CHC-3: 23.3%, CHC-4: 24.1%, CHC-5: 23.8%, the yield of CHC-4 is the highest with 24.1%. It was found to be high, and yields of 20% or more were confirmed in other compositions, resulting in a high overall yield.

Samples Yeild (%, w/w) CHC-1 20.8 CHC-2 22.4 CHC-3 23.3 CHC-4 24.1 CHC-5 23.8

<Experiment 3> Measurement of total polyphenol content

(A) Experimental method

For polyphenol quantification, 1 ml of 1N-Folin - Ciocalteu phenol reagent reagent is added to 3 ml of a 100-fold diluted sample solution, 0.2 ml of 1N HCl is added, 1 ml of saturated Na2CO3 solution is added, mixed, and left at room temperature for 1 hour. After measuring the absorbance at , 640 nm, the polyphenol content was calculated by comparing it with the absorbance value of a standard curve prepared in advance with tannic acid as a standard material.

(B) Experiment result

Phenol compounds are known to exhibit various physiological activities such as antioxidant, anti-cancer, anti-inflammatory and anti-allergic actions by easily binding to proteins and other macromolecules because they have a phenolic hydroxyl group.

In addition, these phenolic compounds exhibit anti-inflammatory activity by regulating enzyme expression as well as enzyme activity involved in inflammatory reactions. Research on natural antioxidants in edible plants consumed on a daily basis is being actively researched, and currently known natural antioxidant nutrients include trace minerals and phenolic compounds such as vitamins such as β-carotene, vitamin E, and vitamin C. Therefore, it is reported that their anticancer effects are also due to their antioxidant capacity.

After designating tannic acid as a reference material, a standard curve was drawn and the polyphenol content of CHC-1 to 5 was measured and shown in Table 3.

CHC-1 (156.32mg/g), CHC-2 (226.58mg/g), CHC-3 (238.25mg/g), CHC-4 (273.82mg/g), CHC-5 (221.97mg/g) showed the highest polyphenol content in CHC-4.

Compositions of extraction Total phenolics
(mg TAE/g) CHC-1 156.32 CHC-2 226.58 CHC-3 238.25 CHC-4 273.82 CHC-5 221.97 1) The total phenolic contents was expressed as milligram of the tannic
acid equivalent (TAE) per gram of extract

<Experiment 4> Measurement of total flavonoid content

(A) Experimental method

Total flavonoid content was determined by the method of Nieva et al. That is, after diluting 100 μl of the sample in 900 μl of 80% ethanol, take 100 μl, mix it with 4.3 ml of 80% ethanol containing 10% aluminum nitrate and 1 μm postassium acetate, and leave it at room temperature for 40 minutes, and then measure the absorbance at 415 nm. Measure. At this time, the total flavonoid content was obtained from a standard curve prepared using quercetin.

(B) Experiment result

Flavonoids have antibacterial, anticancer, antiviral, antiallergic and anti-inflammatory activities, and are reported to show little toxicity, and as it is known that they inhibit oxidation in vivo, interest in the development and utilization of flavonoid-based substances is growing. .

In recent studies, it has been reported that flavonoids contained in natural plants can provide sufficient help in displaying antioxidants and other biological activities in the human body, and their relevance has been demonstrated (Lu et al., 1995; Crespy et al., 2001; Day et al., 2003).

After designating Quercetin as a reference material, a standard curve was drawn, and the flavonoid content of CHC-1 to 5 was measured and shown in Table 4.

CHC-1 (18.45mg/g), CHC-2 (22.08mg/g), CHC-3 (25.41mg/g), CHC-4 (14.22mg/g), CHC-5 (26.89mg/g) showed the highest flavonoid content in CHC-5.

Compositions of extraction Total flavonoids (mg QUE /g) CHC-1 18.45 CHC-2 22.08 CHC-3 25.41 CHC-4 14.22 CHC-5 26.89 1) The total flavonoid contents was expressed as milligram of the quercertin equivalent (QUE) per gram of extract

<Experiment 5> DPPH radical scavenging ability

(A) Experimental method

For DPPH electron donating ability (EDA), 1 ml of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 ml of each sample solution, stirred, left for 30 minutes, and absorbance was measured at 517 nm. . The electron donating ability was expressed as the absorbance reduction rate of the group with and without the sample solution.

Inhibition rate % = [(C-D)-(A-B)] / (C-D) X 100

(B) Experiment result

1,1-diphenyl-2-picrylhydrazyl (DPPH), widely used for antioxidant effect, has stable free radical properties by sharing extra electrons in the molecule. When DPPH solution is mixed with other substances, the more it is reduced, the more purple it is. represents the shape in which it is lost. The antioxidant function can be measured by the ratio of the remaining DPPH through the free radical scavenging activity at the absorbance of 517 nm.

As a result of measuring the DPPH radical scavenging activity of the natural plant composite material extract, it was confirmed as shown in the graph below.

The radical scavenging activity was increased in a concentration-dependent manner, and at a concentration of 100 μg/ml of natural plant composite extract, CHC-1: 81.9%, CHC-2: 92.2%, CHC-3: 90.7%, CHC-4: 91.5%, CHC-5 : It was confirmed that the DPPH radical scavenging ability was the best in CHC-4 at 90.0%.

However, CHC-4 showed a DPPH radical scavenging activity of 68.4%, while CHC-4 was 42.9% at a low concentration of 25㎍/㎖, and this result showed that CHC-5 had a lower concentration of 25㎍/㎖ or less than CHC-4. It means that there is a better antioxidant activity in

CHC-1: CHC-1 extracted with 70% ethanol.

CHC-2: CHC-2 extracted with 70% ethanol.

CHC-3: CHC-3 extracted with 70% ethanol.

CHC-4: CHC-4 extracted with 70% ethanol.

CHC-5: CHC-5 extracted with 70% ethanol.

Vit. C: Vitamin C

ReSult are means ± S.D. of triplicate data.

<Experiment 6> ABTS radical scavenging ability

(A) Experimental method

After preparing 7 mM ABTS buffer and 2.45 mM potassium perSulfate buffer (K ₂ S ₂ O ₈ ), ABTS: K2S2O8 = 2: 1 was mixed in a ratio of 1, and then reacted by blocking light for 12 to 16 hours. Dilute the stock buffer so that the OD value measured at 734 nm becomes 0.70 ± 0.02, add 100 μl of the extract sample diluted by concentration to 100 μl of buffer, and react at room temperature for 6 minutes to measure absorbance at 734 nm, with and without addition It was expressed as the decrease in absorbance of the group.

Inhibition rate % = [(C-D)-(A-B)] / (C-D) X 100

(B) Experiment result

The commonly used ABTS+ radical cation scavenging activity, such as the scavenging activity of DPPH radical, is a reaction between 2,2'-Azino-bis(3-ethylbenzothiazoline-6-Sulfonic acid) diammonium salt (ABTS) and potassium perSulfate to generate ABTS+ radical. It is a method of measuring decolorization to light green as the sample is added, and it was confirmed as shown in the graph below by measuring both hydrogen-donating antioxidant and chain breaking antioxidant.

ABTS+ radical scavenging activity was increased in a concentration-dependent manner, and at a concentration of 100 μg/ml of natural plant composite extract, CHC-1: 89.6%, CHC-2: 96.2%, CHC-3: 99.2%, CHC-4: 99.5%, CHC- 5: It was confirmed that CHC-4 and CHC-5 had the best ABTS+ radical scavenging ability at 99.5%.

CHC-5 showed 90.16% ABTS+ radical scavenging activity at a low concentration of 50 μg/ml, and this result is not superior to the ABTS+ radical scavenging activity of 101% vit-C, a positive control, but considering that vit-C is a single component. It can be inferred that the natural plant composite material has a very good ABTS+ radical scavenging ability.

CHC-1: CHC-1 extracted with 70% ethanol.

CHC-2: CHC-2 extracted with 70% ethanol.

CHC-3: CHC-3 extracted with 70% ethanol.

CHC-4: CHC-4 extracted with 70% ethanol.

CHC-5: CHC-5 extracted with 70% ethanol.

Vit. C: Vitamin C

ReSult are means ± S.D. of triplicate data.

According to the experimental results of <Experiment 3> to <Experiment 6>, CHC-5, which was analyzed to have the best polyphenol content, flavonoid content, and antioxidant efficacy, was confirmed as the final natural plant composite extract, and the following experiments were performed. carried out.

<Experiment 7> Confirmation of cytotoxicity of CHC-5

(A) Cell culture

Each cell was cultured using Dulbeco's modified eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), and subcultured at _{37° C., adapted to a 5% CO 2 incubator.}

(B) MTT assay

Cell viability was measured according to Carmichael's method. Each cell line [Macrophage (Raw 264.7) cell] was aliquoted in a 96-well plate so as to be 0.6∼8×10 ³ cells/well, 0.18ml was prepared by concentration, and 0.02ml was added thereto. Incubated for 24 hours.

The control group was cultured under the same conditions by adding the same amount of distilled water as the sample. Here, 0.02 ml of MTT solution prepared at a concentration of 5 mg/ml was added, incubated for 4 hours, the culture medium was removed, and 0.15 ml of DMSO:Ethanol (1:1) was added to each well and reacted at room temperature for 30 minutes, followed by an ELISA reader. Absorbance was measured at 550 nm.

Cell viability was measured as the decrease in absorbance of the group with and without the sample solution.

(C) Experiment result

The viability of macrophages (Raw 264.7) was measured by MTT assay for reduction of NTT into purple formazan product by mitochondrial dehydrogenase. As a result of confirming the cell viability of CHC-5, which has the best efficacy through enzyme experiments, the survival rate of more than 90% was confirmed up to a concentration of 6-50 μg/ml.

The difference in survival rate was less than 10% when compared to the control group treated with the same concentration of DMEM without the addition of a sample. At a concentration of 50 μg/ml or more of CHC-5, it was confirmed that the cell viability was rather low.

Based on the experimental results, subsequent experiments were conducted at a concentration of 50 μg/ml or less, which confirmed a cell viability of 90% or more. CHC-5 has cytotoxicity to Raw 264.7 at concentrations of 100 and 200 μg/ml, and cell viability was measured to be 69.4 and 46.0%, and it is judged that there is some degree of cytotoxicity at concentrations above 100 μg/ml.

Raw 264.7 cells were treated with 6.25, 12.5, 25, 50, 100, 200㎍/㎖ dissolved in media for 1 h prior to the addition of LPS (1㎍/㎖), and the cells were further incubated for 24 h. Data represent the mean ± S.D. with three separate experiments.

<Experiment 8> Nitric oxide inhibitory ability of CHC-5

(A) Experimental method

The concentration of NO was measured using Griess Reagent for the concentration of nitrite in the culture medium. Raw 264.7 cells were pre-treated with the extract, and after 1 hour, 100ng/ml of LPS was treated and cultured for 18 hours. μl

The same amount of Griess Reagent as 50 μl of the culture medium was added, and after reacting at room temperature for 10 minutes, absorbance was measured at 540 nm with an ELISA reader, and the NO concentration in the culture medium was determined using a standard curve for each concentration of sodium nitrite.

(B) Experiment result

Nitric oxide (NO) is a small, relatively unstable, and toxic inorganic low-molecular radical that is involved in neurotransmission functions, blood coagulation and blood pressure promotion, and the defense function of the host immune system against tumor cells and intracellular parasites.

The enzyme that produces NO (NOS: nitricoxide synthase) is largely classified into two categories: constitutive NOS (c-NOS), which is responsible for NO production for normal physiological functions, and inducible NOS (iNOS), which is induced under special circumstances.

However, NO produced by inflammatory sinus is produced by macrophages stimulated by lipopolysaccharide (LPS), tumor necrosis factor-a (TNF-a), interleukin-1β (IL-1β) or interferon-γ (IFN-γ), It is induced by iNOS produced in hepatocytes and kidney cells.

In order to investigate the effect of the natural plant composite extract on the production of NO, a product of iNOS, the amount of NO production was measured using the Griess reagent. When 1 μg/ml of LPS and extracts of natural plant composites were treated by concentration, NO production increased more than 5 times in the LPS-only group compared to the normal group, but it was confirmed that the NO production amount was significantly reduced in the CHC-5 treatment group, respectively. could

In particular, it was confirmed that the NO production amount was suppressed by 52% compared to the control in the concentration of 50 μg/ml, which is the highest concentration section. Even at a low concentration of 25 μg/ml, the NO production inhibition rate was 48%, which corresponds to 74% of the EGCG efficacy compared to the 65% inhibition rate of EGCG at the same concentration, which means that it has a high anti-inflammatory effect considering that EGCG is a pure single substance. do. In addition, more than 60% of the quantitative target of EGCG efficacy was achieved.

Raw 264.7 cells were cultured with LPS (1㎍/㎖) in the presence or absence of CHC-5 ethanol extracts for 24 h to determine the level of NO. Nor: LPS not induced group, Con: LPS induced group. Data represent the mean ± S.D. with three separate experiments.

<Experiment 9> PGE of CHC-5 ₂ Expression level measurement

(A) Experimental method

To measure the amount of PGE2 in cell culture, a commercial competitive enzyme immunoassay kit was purchased from R&D systems (Minneapolis, MN) and tested. Cells were pre-treated with the extract and treated with 100 ng/ml LPS. After 18 hours, a cell culture medium was obtained and used for PGE2 measurement.

100 μl of each culture medium was loaded onto a 96-well plate coated with goatanti-mouse, and 50 μl of primary antibody solution and 50 μl of PGE2 conjugate were added thereto, followed by incubation at 4°C overnight. After washing 4 times with washing buffer, 200 μl of substrate solution was treated and reacted for 5 to 20 minutes, 50 μl of stop solution was treated, and absorbance was measured at 450 nm.

(B) Experiment result

One of the endotoxins, lipopolysaccharide (LPS), secretes inflammatory mediators such as _{nitric oxide (NO) and prostaglandinE 2} (PGE _{2 ) from Raw 264.7 macrophage.} In the inflammatory state, cyclooxygenase-2 (COX-2) and NO synthase (NOS) are induced to generate excess PGE2 and NO, and various diseases and cancers (carcinogenesis) are promoted.

In particular, NOS, an enzyme that produces NO, and cyclooxygenase (COX), an enzyme that mediates the biosynthesis of various prostaglandins (PGs), are known as important mediators for regulating the inflammatory response.

As NO and PGs are closely related to the inflammatory response, CHC-5, another inflammatory metabolite induced by LPS, was confirmed to have an inhibitory effect on the _{production of PGE 2, another inflammatory metabolite, in the measurement of nitric oxide production inhibitory ability.}

As a result of treating Raw264.7 cells with LPS, the expression level of _{PGE 2 was measured 10 times higher than that of the untreated group.} In the case of CHC-5, when treated with concentrations of 6.25, 12.5, 25, and 50 μg/ml, it was confirmed that the inhibition was proportional to the concentration by 10, 17, 26, and 36%, respectively, compared to the control, and proportional to the concentration of CHC-5 Thus, it was confirmed that the inhibitory effect of _{PGE 2 expression was also increased.}

Production of PGE ₂ were measured in the medium of Raw 264.7 cells clutured with LPS (1㎍/㎖) in the presence or absence of CHC-5 ethanol extracts for 24 h. The amount of PGE ₂ was measured by immunoassay as described in materials and methods. Nor: LPS not induced group, Con: LPS induced group. Data represent the mean ± SD with three separate experiments.

<Experiment 10> CHC-5 hair loss prevention effect test

(A) Cell lines and cell culture

For the cells used in the experiment, human hair dermal papilla cells (HDPC) isolated from the scalp of an adult male in their 50s were distributed from the Immunology Laboratory of the Graduate School of Medicine at Kyungpook National University and used for in vitro evaluation.

The medium used for cell culture was Dulbecco's modified eagle's medium (DMEM, Gibco BRL) with 10% fetal bovine serum (FBS, Sigma) added as a basal medium, and cultured at _{37°C, 5% CO 2 in an incubator.}

(B) Measurement result of cell viability by MTT assay

The survival rate of human dermal papilla cells HDPC (Hair Dermal papilla cell) was confirmed as shown in the graph below as a result of measuring the reduction of NTT into a purple formazan product by the mitochondrial dehydrogenase of the cell by MTT assay.

As a result of checking the cell viability of HDPCl according to the concentration of CHC-5 sample treatment, HDPC cells exhibited more than 80% cell viability when treated with CHC-5 extracts of 3.13, 6.25, and 12.5 μg/ml, and 25, 50, 100 μg/ml At the concentration of ml, the cell viability was 2.9±0.55% and 0±0.28%, respectively, and the viability decreased in a concentration-dependent manner.

At a concentration of 25 μg/ml or more of CHC-5, a low cell viability was confirmed. Based on this result, subsequent experiments were conducted at a concentration of 12.5 μg/ml or less, which confirmed a cell viability of 80% or more.

MTT assay of DP cells treated with CHC-5 extracts. HDPC treated with CHC-5 control, 3.13㎍/㎖, 6.25㎍/㎖, 12.5㎍/㎖., 25㎍/㎖, 50㎍/㎖ and 100㎍/㎖ for 24 h show the reduction of viability in a dose dependent manner(*p<0.005).

(C) Measurement result of HDPC (Hair Dermal papilla cell) cell viability of synthetic surfactants SLS and SLES

MTT assay was used to determine the toxic effect of synthetic anionic surfactants, SLS (Sodium lauryl Sulfate) and SLES (Sodium laureth Sulfate), which are widely used in cleaning products, on the viability of DP cells.

As a result of comparing cell viability by treating SLS and SLES in HDPC for 24 hours by concentration, as shown in the graph below, the control group not treated with SLS or SLES in HDPC showed 100% viability, but SLS, SLES 100㎍/㎖ During treatment, the survival rate was decreased in a concentration-dependent manner to 27% and 25% in HDPC, respectively.

Even at a low concentration of 10 μg/ml, the cell viability dropped to less than 80%, indicating that both SLS and SLES had some cytotoxicity, and SLES was confirmed to be slightly more toxic than SLS.

(D) Verification result of human dermal papilla cell expression ability of CHC-5

IGF-1 is known as a representative growth factor that promotes the growth of hair follicles and epithelial cells. In addition, it was reported that the addition of IGF-1 when hair follicles transition from the growth phase to the catagen phase inhibits the transition to the catagen phase. It prevents apoptosis of dermal papilla cells, and male hormone directly affects the production of IGF-1 in dermal papilla cells.

As a result of measuring the activity of IGF-1 by treating the dermal papilla cells with CHC-5 extracts of various concentrations, it was confirmed as shown in the graph below.

The activity of IGF-1, a growth factor of hair follicles and epithelial cells, was 75 ± 5.4%, 85 ± 0.9%, and 95 ± 7.1% when the CHC-5 extracts were treated with 3.13, 6.25, and 12.5 μg/ml of 12.5 μg/ml. It showed the highest activity of IGF-1 at the concentration.

Since IGF-1 activity increases in a concentration-dependent manner of the CHC-5 extract, it is considered that the CHC-5 extract is effective in promoting hair growth by inducing the growth of hair follicle cells.

(E) 5a-reductase activity inhibition test result of CHC-5 (Experiment is performed to index the growth rate)

Testosterone, a male hormone, is converted into DHT when it is activated by 5α-reductase in hair follicles. When the converted DHT binds to the androgen receptor on the dermal papilla membrane in the frontal and parietal regions, a cell destruction signal is transmitted to the DNA of the hair mother cells, and the apoptosis factors BMP, DKK-1, and TGF-β are produced. .

The cell necrosis factor generated in this way attacks and destroys the hair cells, shortening the hair growth period and causing the catagen phase to come at an early stage.

Type 1 5α-reductase is mainly secreted to the sebaceous glands and type 2 is distributed in the outer hair follicle and dermal papilla of the hair follicle. Type 2 enzyme is the main cause of hair loss. Finasteride is known to be effective in preventing and treating hair loss by inhibiting type 2 5α-reductase.

Therefore, if 5α-reductase can be inhibited or removed, androgenetic alopecia such as androgenetic alopecia can be prevented or treated, so the evaluation of 5α-reductase inhibitory ability is an important indicator to know whether or not hair loss prevention effect exists.

Therefore, in order to confirm the inhibitory effect of CHC-5 on 5α-reductase activity compared to Minoxidil (MI), which is a conventional hair loss treatment, cytoplasmic protein was extracted from hair follicle cells and the activity of 5α-reductase was investigated.

As a result of expressing the activity of 5α-reductase in the dermal papilla cells treated with the sample as a percentage compared to the activity of 5α-reductase in the dermal papilla cells not treated with the sample, 5 μg/ml of Minoxidil, which is known to inhibit the activity of 5α-reductase At the concentration of 56 ± 5.7% of activity was measured.

On the other hand, CHC-5 showed 97 ± 3.8%, 68 ± 13.2% and 55 ± 10.8% of activity at 3.13, 6.25, and 12.5 μg/ml, respectively, so that it was more effective than 5α-reductase at 12.5 μg/ml of Minoxidil (MI). It was possible to confirm the results of the inhibition of the activity as shown in the graph below.

In addition, as the concentration of CHC-5 increased, the expression of 5α-reductase decreased, and when the amount of CHC-5 added was 12.5 μg/ml, it was confirmed that 5α-reductase was reduced by 45.0% compared to the control without CHC-5.

From this result, the expression of 5α-reductase was significantly inhibited in proportion to the concentration of CHC-5, and the inhibitory efficacy was 45% compared to the control at a low concentration of 12.5 μg, which was not highly toxic.

Since suppression of 5α-reductase expression is known to affect the proliferation of hair follicle cells, it can be interpreted indirectly that the proliferation effect of hair follicle cells increases in proportion to 45%.

According to the experimental results of <Experiment 7> to <Experiment 10>, CHC-5, whose physiological activity and anti-hair loss efficacy was confirmed, was applied to shampoo, serum, and liquid-type cosmetic composition for hair loss care, and the following experiments were performed.

<Cosmetic composition for hair loss care serum according to formulation application experiment of CHC-5>

With respect to 100 parts by weight of deionized water,

1.2- hexanediol 2.00 parts by weight; 1.00 parts by weight of panthenol; Produce-400 0.50 to 5.00 parts by weight; 0.30 to 2.00 parts by weight of niacinamide; 0.10 to 0.50 parts by weight of copper tripeptide-1 (product name: Apep CTP-10); 0.06 to 0.10 parts by weight of biotin; 0.03 parts by weight of disodium EDTA; 0.03 to 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 to 0.30 parts by weight of pyridoxine HCl; Ammonium Acryloyldimethyltaurate (Ammonium Acryloyldimethyltaurate VP copolyme) 0.05 to 0.10 parts by weight; 1.00 parts by weight of L-arginine; 0.50 parts by weight of citric acid; 0.60 parts by weight of sodium citrate dihydrate; 0.10 to 0.50 parts by weight of alanine/histidine/lysine polypeptide copper HCL (product name: Apep Actigrowth); 10.00 parts by weight of butylene glycol; 0.10 parts by weight of olive oil; 0.03 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 to 0.15 parts by weight of salicylic acid; 0.60 to 0.80 parts by weight of PEG-60 (hydrogenated castor oil); Lemon essential oil 0.08 parts by weight; CHC-5 (natural plant composite material extract) 1.60 to 5.00 parts by weight; includes

<Cosmetic composition for hair loss care shampoo according to formulation application experiment of CHC-5>

With respect to 100 parts by weight of deionized water,

Disodium Laureth Sulfosuccinate 10.00 to 40.00 parts by weight; 5.00 to 25.00 parts by weight of lauryl glucoside; 5.00 to 10.00 parts by weight of sodium cocoyl apple amino acid; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 4.00 to 10.00 parts by weight of glycerin; 4.00 to 5.00 parts by weight of sorbitol; Decylglucoside 5.00 parts by weight; Polyquaternium-67 0.10 to 1.00 parts by weight; Polyquaternium-10 0.10 to 0.26 parts by weight; 0.20 to 0.24 parts by weight of guarhydroxypropyltriammonium chloride; 0.30 to 2.00 parts by weight of niacinamide; 0.06 to 0.10 parts by weight of biotin; Produce-400 0.50 to 3.00 parts by weight; 0.03 parts by weight of disodium EDTA; 3.00 to 10 parts by weight of sodium lauroyl glutamate; 2.00 to 3.00 parts by weight of glucamate; 5.00 parts by weight of cocamidopropyl betaine; 1.2-hexanediol 2.00 parts by weight; 0.50 parts by weight of moringa oil: 0.30 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 parts by weight of olive oil; 3.00 parts by weight of liquid silk amino acid; CHC-5 (natural plant composite material extract) 1.60 to 3.00 parts by weight; includes

<Cosmetic composition for hair loss care liquid according to formulation application experiment of CHC-5>

With respect to 100 parts by weight of deionized water,

5.00 parts by weight of sorbitol; 1.2-hexanediol 2.00 parts by weight; 0.50 to 2.00 parts by weight of niacinamide; PCA-Na (Na-pyrolidonecarboxylate) 5.00 to 10.00 parts by weight; Produce-400 3.00 parts by weight; 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 parts by weight of adenosine; Biotin 0.05 to 0.10 parts by weight; 0.01 parts by weight of lactobionic acid; 0.05 parts by weight of gluconolactone; 5.00 parts by weight of ethanol; 0.05 parts by weight of hinokitiol oil; 0.15 parts by weight of menthol; 0.05 parts by weight of salicylic acid; 0.01 parts by weight of mandelic acid; 0.25 to 0.30 parts by weight of lemon essential oil; 0.70 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tocopheryl acetate; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; CHC-5 (natural plant composite material extract) 3.00 to 5.00 parts by weight; includes

<Experiment 11> Test of pH measurement over time of a cosmetic composition for hair loss care applied with CHC-5

(A) Results of pH measurement over time of a cosmetic composition for hair loss care serum to which CHC-5 is applied

In order to predict the chemical stability and physical stability over time of the cosmetic composition for hair loss care serum to which CHC-5 is applied, a pH measurement experiment was performed over time, and it was confirmed as shown in the graph below.

That is, as a result of measuring the pH of the test product stored at 45° C. for 60 days, it was confirmed that the pH was stable over time.

(B) pH measurement result over time of the cosmetic composition for hair loss care shampoo to which CHC-5 is applied

In order to predict the chemical stability and physical stability over time of the cosmetic composition for hair loss care shampoo to which CHC-5 is applied, it was confirmed as shown in the graph below as a result of performing a pH measurement experiment over time.

That is, as a result of measuring the pH of the test product stored at 45° C. for 60 days, it was confirmed that the pH was stable over time because there was no large deviation in the pH.

(C) Results of pH measurement over time of a cosmetic composition for hair loss care liquid to which CHC-5 is applied

In order to predict the chemical stability and physical stability over time of the cosmetic composition for hair loss care liquid to which CHC-5 is applied, it was confirmed as shown in the graph below as a result of performing a pH measurement experiment over time.

That is, as a result of measuring the pH of the test product stored at 45° C. for 60 days, it was confirmed that the pH was stable over time.

<Experiment 12> Test for measuring viscosity over time of a cosmetic composition for hair loss care to which CHC-5 is applied

(A) Viscosity measurement result over time of the cosmetic composition for hair loss care serum to which CHC-5 is applied

In order to predict the chemical stability and physical stability over time of the cosmetic composition for hair loss care serum to which CHC-5 is applied, the viscosity was measured over time under the condition of 12 rpm, and it was confirmed as shown in the graph below.

That is, it was confirmed that the initial viscosity was 0 under the condition of 12 rpm and the viscosity was maintained at 0 over time.

(B) Viscosity measurement result over time of the cosmetic composition for hair loss care shampoo to which CHC-5 is applied

In order to predict the chemical stability and physical stability over time of the cosmetic composition for hair loss care shampoo to which CHC-5 is applied, it was confirmed as shown in the graph below as a result of measuring the viscosity over time.

That is, as a result of measuring the viscosity of the sample stored at 45° C. for 60 days, it was observed that the viscosity, which was initially 3,000 cps, was maintained without significant deviation, confirming that chemical and physical stability were secured over time.

(C) Viscosity measurement result over time of the cosmetic composition for hair loss care liquid to which CHC-5 is applied

In order to predict the chemical stability over time of the cosmetic composition for hair loss care liquid to which CHC-5 is applied, it was confirmed as shown in the graph below as a result of measuring the viscosity over time.

That is, it was confirmed that the initial viscosity was 0 under the condition of 12 rpm and the viscosity was maintained at 0 over time.

<Experiment 13> Temperature stability experiment of cosmetic composition for hair loss care applied with CHC-5

(A) Temperature stability test result of cosmetic composition for hair loss care serum to which CHC-5 is applied

The cosmetic composition for hair loss care serum applied with CHC-5 and functional peptide components was evaluated for temperature stability for 2 months (60 days) under an accelerated condition at 45°C, and the results shown in Table 5 below were obtained.

As a result of the experiment, it was confirmed that it was stably maintained without significant change for 2 months (60 days).

In general, if it is stably maintained for 3 months (90 days) under an accelerated condition of 45°C, it is known empirically that it can be stable for about 3 years during commercial distribution. It can be said that distribution has secured sufficient stability.

Temp.
Day CHC-5 applied hair loss care serum 4℃ 25℃ 38℃ 45℃ ultraviolet ray One O O O O O 3 O O O O O 5 O O O O O 7 O O O O O 15 O O O O O 30 O O O O O 60 O O O O O

(B) Temperature stability test result of cosmetic composition for hair loss care shampoo to which CHC-5 is applied

For the cosmetic composition for hair loss care shampoo to which CHC-5 and functional peptides are applied, temperature stability was evaluated for 2 months (60 days) under an accelerated condition of 45°C in order to investigate the spontaneous deterioration over time and chemical and physical changes. As shown in Table 6, it was confirmed that it was stably maintained without significant change for 2 months (60 days).

Temp.
Day CHC-5 applied hair loss care shampoo 4℃ 25℃ 38℃ 45℃ ultraviolet ray One O O O O O 3 O O O O O 5 O O O O O 7 O O O O O 15 O O O O O 30 O O O O O 60 O O O O O

(C) Temperature stability test result of cosmetic composition for hair loss care liquid applied with CHC-5

For the cosmetic composition for hair loss care liquid to which CHC-5 and functional peptides are applied, temperature stability was evaluated for 2 months (60 days) under an accelerated condition of 45°C in order to investigate spontaneous deterioration over time and chemical and physical changes. As shown in Table 7, it was confirmed that it was stably maintained without significant change for 2 months (60 days).

Temp.
Day CHC-5 applied hair loss care liquid 4℃ 25℃ 38℃ 45℃ ultraviolet ray One O O O O O 3 O O O O O 5 O O O O O 7 O O O O O 15 O O O O O 30 O O O O O 60 O O O O O

<Experiment 14> Stability test according to temperature cycling of a cosmetic composition for hair loss care applied with CHC-5

(A) Test results of stability theory according to temperature cycling of a cosmetic composition for hair loss care serum to which CHC-5 is applied

After storing the cosmetic composition for hair loss care serum to which CHC-5 and functional peptide ingredients are applied at -15, -10, -5, 0, 5, 10, 15, 25, 37.5, and 40°C for 24 hours, As a result of observing the stability, it was confirmed as shown in Table 8 below.

As a result of the experiment, it was visually confirmed that there was no phase separation, discoloration, or discoloration in all 6 cycles.

Cycle One 2 3 4 5 6 Stability O O O O O O O: Stable X: Unstable

(B) Test result of stability theory according to temperature cycle of cosmetic composition for hair loss care shampoo to which CHC-5 is applied

After storing the cosmetic composition for hair loss care shampoo to which CHC-5 and functional peptide ingredients are applied at -15, -10, -5, 0, 5, 10, 15, 25, 37.5, and 40°C for 24 hours, As a result of observing stability, it was confirmed as shown in Table 9 below.

As a result of the experiment, it was visually confirmed that there was no phase separation, discoloration, or discoloration in all 6 cycles.

Cycle One 2 3 4 5 6 Stability O O O O O O O: Stable X: Unstable

(C) Test result of stability theory according to temperature cycle of the cosmetic composition for hair loss care liquid to which CHC-5 is applied

After storing the cosmetic composition for hair loss care liquid applied with CHC-5 and functional peptides at -15, -10, -5, 0, 5, 10, 15, 25, 37.5, and 40°C for 24 hours, As a result of observing stability, it was confirmed as shown in Table 10 below.

As a result of the experiment, it was visually confirmed that there was no phase separation, discoloration, or discoloration in all 6 cycles.

Cycle One 2 3 4 5 6 Stability O O O O O O O: Stable X: Unstable

<Experiment 15> Preservative power test of cosmetic composition for hair loss care applied with CHC-5

(A) Bacterial culture

Escherichia coli and Staphylococcus epidermis , a type of Bacteria As a liquid medium, nutrient broth (NB) Difco Lab. (Sparks, USA) was used, and tryptic soy broth (TSB) was used as a liquid medium for Staphylococcus aureus (KCTC 1621), and brain heart as a liquid medium for culturing Candida albicans and Aspergillus niger, yeast and mold types. Using infusion (BHI), agar was added to the liquid medium as a solid medium.

E. coli, S. epidermidis, and S. aureus were cultured at 37℃ incubation for 18~24 hours, and C. albicans and A. niger were cultured at 25~30℃ incubation for 3-5 days.

(B) Measurement of the number of bacteria

For the measurement of the number of bacteria, the culture strain was diluted in a liquid medium (medium 100ml + culture solution) The initial number of bacteria Bacteria was 1×10 ⁶ CFU/g, and 1ml of the diluted solution was inoculated into 100ml of Sample and then dispensed into a solid medium to give Bacteria 24 After incubation for ~48 hours, the yeast and mold were cultured for 48~72 hours, and then the number of bacteria was measured using a plate with 30~300 bacteria.

Bacteria count is calculated by multiplying the number of colonies × dilution. If the number of viable cells could not be determined, the number of bacteria was counted using TNTC (Too numberous to counter).

(C) Experimental method

In order to verify the antiseptic system that maximizes the antibacterial power of natural ingredients, a challenge test was performed by dividing the cosmetic composition for hair loss care applying CHC-5 and functional peptides into serum, shampoo and liquid type.

The composition of the natural preservative system is as follows.

CHC-5 1,2 Hexanediol Salicylic acid EDTA-2Na Hair Loss Care Serum A 5.0% 4.0% 0.15% 0.02% Hair Loss Care Serum B 5.0% 2.0% 0.15% 0.02% Hair Loss Care Serum C 5.0% 1.0% 0.15% 0.02% Hair Loss Care Serum D 5.0% 0.0% 0.50% 0.02%

CHC-5 1,2 Hexanediol Hair loss care shampoo A 5.0% 4.0% Hair Loss Care Shampoo B 5.0% 2.0% Hair Loss Care Shampoo C 5.0% 1.0%

CHC-5 1,2 Hexanediol Salicylic acid Ethanol Hair Loss Care Liquid A 5.0% 4.0% 0.05% 5.0% Hair Loss Care Liquid B 5.0% 2.0% 0.05% 5.0% Hair Loss Care Liquid C 5.0% 1.0% 0.05% 5.0% Hair Loss Care Liquid D 5.0% 0.0% 0.05% 5.0%

(D) Preservative test result of cosmetic composition for hair loss care serum applied with CHC-5

Microorganism Initial Inoculum/ml ATCC. No Pa: Pseudomonas aeruginosa 1 X 10 ⁶ Gram negative bacteria 10145 Sa: Staphylococcus aureus 1 X 10 ⁶ Gram positive bacteria 6538 Ec: Escherichia coli 1 X 10 ⁶ Gram negative bacteria 8739 Ca: Candida albicans 1 X 10 ⁵ Yeast 10231 An: Aspergillus niger 1 X 10 ⁵ Black mold 16404

Hair Loss Care Serum A Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An Hair Loss Care Serum B Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa 1 X 10 ³ ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An Hair Loss Care Serum C Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa 1 X 10 ³ ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An Hair Loss Care Serum D Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa 1 X 10 ³ ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An

(E) Preservative test result of cosmetic composition for hair loss care shampoo applied with CHC-5

Microorganism Initial Inoculum/ml ATCC. No Pa: Pseudomonas aeruginosa 1 X 10 ⁶ Gram negative bacteria 10145 Sa: Staphylococcus aureus 1 X 10 ⁶ Gram positive bacteria 6538 Ec: Escherichia coli 1 X 10 ⁶ Gram negative bacteria 8739 Ca: Candida albicans 1 X 10 ⁵ Yeast 10231 An: Aspergillus niger 1 X 10 ⁵ Black mold 16404

Hair loss care shampoo A Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa 2 X 10 ³ ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An

Hair Loss Care Shampoo B Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An

Hair Loss Care Shampoo C Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An

(F) Preservative test result of cosmetic composition for hair loss care liquid to which CHC-5 is applied

Microorganism Initial Inoculum/ml ATCC. No Pa: Pseudomonas aeruginosa 1 X 10 ⁶ Gram negative bacteria 10145 Sa: Staphylococcus aureus 1 X 10 ⁶ Gram positive bacteria 6538 Ec: Escherichia coli 1 X 10 ⁶ Gram negative bacteria 8739 Ca: Candida albicans 1 X 10 ⁵ Yeast 10231 An: Aspergillus niger 1 X 10 ⁵ Black mold 16404

Hair Loss Care Liquid A Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An Hair Loss Care Liquid B Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca ND ND ND ○ ND ND ND ○ An Hair Loss Care Liquid C Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa 1 X 10 ³ ND ND ○ ND ND ND ○ Ec Sa Ca 1 X 10 ³ ND ND ○ ND ND ND ○ An Hair Loss Care Liquid D Day 1 (24h) 3 7 Experiment result 14 21 28 Experiment result Dilution 10 ^-3 10 ^-0 10 ^-0 preservative 10 ^-0 10 ^-0 10 ^-0 antiseptic
effectiveness Pa ND ND ND ○ ND ND ND ○ Ec Sa Ca 6 X 10 ³ ND ND ○ ND ND ND ○ An

As a result of the preservative experiment as described above, it was determined that the preservative power was appropriate because the microorganisms were killed on the 3rd day in the composition of all the cosmetic compositions for hair loss care to which CHC-5 was applied.

<Experiment 16> Quality and efficacy evaluation experiment of hair care products to which the cosmetic composition for hair loss care applied with CHC-5 is applied

(A) Experimental method

The survey personnel selected for the quality and efficacy evaluation test of hair care products (serum, shampoo, liquid) applied with the cosmetic composition for hair loss care applied with CHC-5 and functional peptide ingredients receive sufficient training in advance to determine the feeling of use. More than 21 men and women in their 20s and 40s (including students with oily skin) were selected and conducted.

Test items were evaluated with 7 to 8 items depending on the product among feeling of use, cleansing power, foaming power, moisturizing power, hair condition, skin irritation, scent, and feeling after use, and the questionnaire was evaluated out of 7 points { very good (7 points); Relatively good (6 points), slightly good (5 points), neither (4 points), slightly bad (3 points), relatively bad (2 points), very bad (1 point)}.

(B) CHC-5 applied hair loss care serum evaluation result summary report

(C) CHC-5 applied hair loss care shampoo evaluation result summary report

(D) Summary report of evaluation results of hair loss care liquid applied with CHC-5

<Experiment 17> Skin safety and skin irritation test of products to which a cosmetic composition for hair loss care applied with CHC-5 is applied

(A) Experimental method

In order to test the skin safety and skin irritation of products (serum, shampoo, liquid) applied with a cosmetic composition for hair loss care to which CHC-5 and functional peptides are applied, we applied the methods of Waggoner et al., Matsumura, etc. to sell materials for scalp care products. After sealing the patch for 48 hours using Finn chamber on Scanpor tape on the skin of the inner part, the reaction of the skin was visually checked.

For this experiment, 4 male and 7 female students with the ability to discriminate the feeling of use after undergoing sufficient education in advance were selected as survey agents and the experiment was conducted.

reaction Expression negative - Imperfection erythema ± Erythema + Small blister, Papule, Edema ++ Big blister, Necrosis +++

Each of the chambers a, b, c, and d of the Finn chamber on Scanpor tape was tested after placing the finished product in the following order.

chamber a: hair serum products

chamber b: hair shampoo products

chamber c: hair liquid product

chamber d: CHC-5 5% solution

(B) Experiment result

As a result of the experiment on 11 people, all showed negative (-) except for one person showing a hair liquid product and another person showing a false positive (±) in the hair shampoo product, so it could be judged as a non-irritating product safe for the skin. .

<Experiment 18> Clinical trial evaluation of a product to which a cosmetic composition for hair loss care applied with CHC-5 is applied (Clinical trials are ongoing at an external clinical company certified by the Ministry of Food and Drug Safety)

(A) Photo data of hair loss alleviation evaluation progress (45 degree photo)

(B) Photo data of hair loss alleviation evaluation progress (photo taken at 90 degrees)

(C) Photo data of hair density evaluation for hair loss relief

As described above, the cosmetic composition for hair loss care of the present invention normalizes the pH of the alkalized scalp, and as the scalp keratin softening and removal ingredients are mixed, the penetration of the active ingredient into the scalp is increased, the sebum and wastes of the scalp are removed, and the scalp is regenerated. has a helping effect.

Above, the embodiment of the present invention is not implemented only through the above-described apparatus and/or operation method, but through a program for realizing a function corresponding to the configuration of the embodiment of the present invention, a recording medium in which the program is recorded, etc. It may be implemented, and such an implementation can be easily implemented by an expert in the technical field to which the present invention belongs from the description of the above-described embodiments.

In addition, although the embodiment of the present invention has been described in detail, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the right.

Claims (5)

With respect to 100 parts by weight of deionized water,
1.2- hexanediol 2.00 parts by weight; 1.00 parts by weight of panthenol; Produce-400 0.50 to 5.00 parts by weight; 0.30 to 2.00 parts by weight of niacinamide; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.06 to 0.10 parts by weight of biotin; 0.03 parts by weight of disodium EDTA; 0.03 to 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 to 0.30 parts by weight of pyridoxine HCl; Ammonium acryloyldimethyltaurate (Ammonium Acryloyldimethyltaurate VP copolymer) 0.05 to 0.10 parts by weight; 1.00 parts by weight of L-arginine; 0.50 parts by weight of citric acid; 0.60 parts by weight of sodium citrate dihydrate; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 10.00 parts by weight of butylene glycol; 0.10 parts by weight of olive oil; 0.03 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 to 0.15 parts by weight of salicylic acid; 0.60 to 0.80 parts by weight of PEG-60 (hydrogenated castor oil); Lemon essential oil 0.08 parts by weight; 1.60 to 5.00 parts by weight of natural plant composite extract; A cosmetic composition for hair loss care to which a natural plant composite material extract is applied, characterized in that it comprises a.
With respect to 100 parts by weight of deionized water,
Disodium Laureth Sulfosuccinate 10.00 to 40.00 parts by weight; 5.00 to 25.00 parts by weight of lauryl glucoside; 5.00 to 10.00 parts by weight of sodium cocoyl apple amino acid; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 4.00 to 10.00 parts by weight of glycerin; 4.00 to 5.00 parts by weight of sorbitol; Decylglucoside 5.00 parts by weight; Polyquaternium-67 0.10 to 1.00 parts by weight; Polyquaternium-10 0.10 to 0.26 parts by weight; 0.20 to 0.24 parts by weight of guarhydroxypropyltriammonium chloride; 0.30 to 2.00 parts by weight of niacinamide; 0.06 to 0.10 parts by weight of biotin; Produce-400 0.50 to 3.00 parts by weight; 0.03 parts by weight of disodium EDTA; 3.00 to 10 parts by weight of sodium lauroyl glutamate; 2.00 to 3.00 parts by weight of glucamate; 5.00 parts by weight of cocamidopropyl betaine; 1.2-hexanediol 2.00 parts by weight; 0.50 parts by weight of moringa oil: 0.30 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tea tree oil; 0.20 parts by weight of menthol; 0.10 parts by weight of olive oil; 3.00 parts by weight of liquid silk amino acid; 1.60 to 3.00 parts by weight of natural plant composite extract; A cosmetic composition for hair loss care applied with a natural plant composite material extract, comprising:
With respect to 100 parts by weight of deionized water,
5.00 parts by weight of sorbitol; 1.2-hexanediol 2.00 parts by weight; 0.50 to 2.00 parts by weight of niacinamide; PCA-Na (Na-pyrolidonecarboxylate) 5.00 to 10.00 parts by weight; Produce-400 3.00 parts by weight; 0.10 parts by weight of dipotassium glycyrrhizite; 0.10 parts by weight of adenosine; Biotin 0.05 to 0.10 parts by weight; 0.01 parts by weight of lactobionic acid; 0.05 parts by weight of gluconolactone; 5.00 parts by weight of ethanol; 0.05 parts by weight of hinokitiol oil; 0.15 parts by weight of menthol; 0.05 parts by weight of salicylic acid; 0.01 parts by weight of mandelic acid; 0.25 to 0.30 parts by weight of lemon essential oil; 0.70 parts by weight of PEG-60 (hydrogenated castor oil); 0.10 parts by weight of tocopheryl acetate; 0.10 to 0.50 parts by weight of copper tripeptide-1; 0.10 to 0.50 parts by weight of Alanine/Histidine/Lysine Polypeptide Copper HCl; 3.00 to 5.00 parts by weight of a natural plant composite material extract; A cosmetic composition for hair loss care to which a natural plant composite material extract is applied, characterized in that it comprises a.
4. The method according to any one of claims 1 to 3,
The natural plant composite material extract,
A cosmetic composition for hair loss care to which a natural plant complex extract is applied, characterized in that it contains any one or more of mistletoe, fig, gold hwagyu, wheat sprout, yeonkyo, red peony, baeksan tea, and green tea.
5. The method of claim 4,
The natural plant composite material extract,
5% by weight mistletoe; figs 5% by weight; 25% by weight of Keumhwa Beef; wheat germ 5% by weight; 15% by weight per year; About 15% by weight of red peony; Baeksan tea 5% by weight; Green tea 20 to 25% by weight; A cosmetic composition for hair loss care to which a natural plant composite material extract is applied, characterized in that it comprises a.