KR102176983B1 - Method of manufacturing cosmetic composition for acnes improvement and cosmetic composition for acnes improvement produced by the same - Google Patents

Abstract

The present invention relates to a method for preparing a cosmetic composition for improving acne, which has antioxidant, anti-inflammatory, and antibacterial effects using a natural plant complex, and has improved feeling and stability, and to a cosmetic composition for improving acne prepared thereby, Schisandra chinensis, fig, mistletoe, A manufacturing step of preparing a natural plant complex by mixing camphor ginseng, hawthorn, fat, and pine needles; And it characterized in that it comprises an extraction step of extracting the natural plant complex extract from the natural plant complex using a solvent.

Description

A method of manufacturing a cosmetic composition for improving acne, and a cosmetic composition for improving acnes manufactured by the method TECHNICAL FIELD [Method of manufacturing cosmetic composition for acnes improvement and cosmetic composition for acnes improvement produced by the same}

The present invention relates to a method for preparing a cosmetic composition for improving acne and to a cosmetic composition for improving acne prepared thereby, and in particular, a cosmetic composition for improving acne with improved feeling of use and stability, having antioxidant, anti-inflammatory, and antibacterial effects using a natural plant compound It relates to a manufacturing method and a cosmetic composition for improving acne manufactured thereby.

Acne is a disease caused by abnormalities in the sebaceous glands that make sebum, and the sebum secreted from the sebaceous glands is discharged to the skin surface through pores, but excessive sebum is produced due to adolescence, pregnancy, drugs, stress, etc. It will happen if.

Such acne shows various clinical symptoms due to the complex action of various factors, and four mechanisms are important in its occurrence factor, including increased sebum secretion, follicular hyper-keratinization, and Propionibacterium (P. ) Acnes colony formation and inflammatory reactions are included.

In addition, genetic factors and environmental factors are known to be involved, and too much oil or cosmetics that seal pores, friction or mechanical irritation, stress, and excessive sweating are also suggested as factors that cause or worsen acne.

In the cosmetic industry, a number of products using natural products are being developed to reduce skin irritation caused by various chemical substances, and natural ingredients not only have fewer side effects on the skin, as well as the recent increase in consumer response to cosmetics using natural ingredients. As a raw material, the value of development is further increasing.

Korean Patent Registration No. 10-1039532 (registered on June 1, 2011) describes a cosmetic composition for improving acne containing a natural antibacterial complex composed of lotus leaf, branch, and phalanx extract. According to the disclosed technology, a natural antibacterial complex obtained by mixing 40 to 80% by weight of lotus leaf hot water extract, 10 to 50% by weight of Jibuja hot water extract, and 10 to 50% by weight of hot water extract of phalanx is mixed with 0.0001 to 20.0% by weight based on the total weight of the cosmetic composition. It is characterized by containing.

Korean Patent Registration No. 10-1934281 (registered on December 26, 2018) describes a cosmetic composition for improving acne containing natural ingredients and an ampoule-type liquid cosmetic using the same, and according to the disclosed technology, it is possible to calm acne occurring on the skin. 90 parts by weight of the first composition prepared to make it; 7 parts by weight of the second composition prepared for moisturizing the skin; 0.5 parts by weight of the third composition prepared to prevent skin aging; 3 parts by weight of the fourth composition as a natural preservative; And 1 part by weight of a fifth composition provided to promote skin regeneration to remove acne scars, wherein the first composition includes 1 to 100 parts by weight of green tea water; 1 to 3 parts by weight of Gamguk flower extract; 1 to 3 parts by weight of Gosam extract; Eoseongcho extract fermented filtrate 1 to 3 parts by weight; 0.1 to 1 part by weight of Melaleuca alternifolia extract; 0.01 to 1 part by weight of magnool; 0.1 to 1 part by weight of totaroll; 0.01 to 0.5 parts by weight of green tea catechin; 0.1 to 1 part by weight of propolis extract; 1 to 5 parts by weight of cedar leaf extract; And 1 to 5 parts by weight of lotus leaf extract, and the second composition includes 1 to 10 parts by weight of witch hazel extract; 0.1 to 1 part by weight of glycerin; And 0.1 to 1 parts by weight of sodium hyaluronate, and the third composition includes 0.1 to 5 parts by weight of palmitoyl tripeptide-1; 0.1 to 5 parts by weight of palmitoyl tripeptide-7; 0.1 to 5 parts by weight of carnosine; And 0.1 to 5 parts by weight of glutathione, and the fourth composition includes 0.1 to 1 part by weight of golden root extract; And 0.1 to 1 parts by weight of a peony root extract, and the fifth composition includes 1 to 5 parts by weight of a birch extract; And Ecklonia cava extract 0.1 to 3 parts by weight; characterized in that it contains.

As described above, the conventional cosmetic composition for improving acne has a problem in that the effect of natural substances is insignificant, or even if there is an effect, it may cause skin irritation, inflammation, etc. to cause side effects on the skin.

Korean Patent Registration No. 10-1039532 Korean Patent Registration No. 10-1934281

The technical task to be achieved by the present invention is to solve the above-described problems, and has antioxidant, anti-inflammatory, and antibacterial effects using a natural plant complex that is a mixture of Schisandra chinensis, figs, mistletoe, camphor ginseng, hawthorn, fat, and pine needles. It provides a method for producing a cosmetic composition for improving acne with improved feeling and stability, and a cosmetic composition for improving acne prepared thereby.

In order to solve this problem, according to one feature of the present invention, a manufacturing step of preparing a natural plant complex by mixing schisandra, fig, mistletoe, camphor ginseng, hawthorn, branch, and pine needles; And it provides a method for preparing a cosmetic composition for improving acne comprising an extraction step of extracting the natural plant complex extract from the natural plant complex using a solvent.

In one embodiment, the manufacturing step is, 4 to 22% by weight of Schisandra, 8 to 11% by weight of figs, 8 to 11% by weight of mistletoe, 14 to 17% by weight of camphor ginseng, 16 to 19% by weight of hawthorn, 16 to 19% by weight of fat %, by mixing 16 to 19% by weight of pine needles to prepare a natural plant complex.

In one embodiment, the extraction step is characterized in that the natural plant complex extract is extracted by repeating the process of immersing the natural plant complex in the solvent at room temperature for 24 hours three times.

In one embodiment, after the extraction step, it characterized in that it further comprises a drying step of filtering and concentrating the natural plant complex extract and freeze-drying.

According to another feature of the present invention, there is provided a cosmetic composition for improving acne prepared by the above-described method for preparing a cosmetic composition for improving acne.

According to the present invention, by preparing a cosmetic composition for improving acne by using a natural plant compound mixed with schizandra, fig, mistletoe, camphor ginseng, hawthorn, fat, and pine needles, the antioxidant, anti-inflammatory and antibacterial components contained in each natural plant are increased. Through the action, not only is it excellent in the treatment and prevention of acne, but there is little irritation to the skin, and the feeling of use, moisturizing power, absorption power, and stability are improved.

1 is a flow chart illustrating a method of manufacturing a cosmetic composition for improving acne according to a first embodiment of the present invention.
2 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a second embodiment of the present invention.
3 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a third embodiment of the present invention.
4 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a fourth embodiment of the present invention.
5 is a flow chart illustrating a method of manufacturing a cosmetic composition for improving acne according to a fifth embodiment of the present invention.
6 is a view showing the measurement result of Superoxide anion radical scavenging ability of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
7 is a view showing the ABTS radical scavenging ability measurement result of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
8 is a view showing the result of measuring the superoxide anion radical scavenging ability of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
9 to 12 are diagrams showing the results of measuring the antibacterial activity of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
13 is a view showing the cell viability measurement result of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
14 is a view showing the result of measuring the Nitric oxide inhibitory ability of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.
15 is a view showing the result of measuring the secretion of Pro-inflammatory cytokine of the cosmetic composition for improving acne prepared according to the first embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art can easily implement the present invention. However, since the description of the present invention is merely an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, since the embodiments can be modified in various ways and have various forms, the scope of the present invention should be understood to include equivalents capable of realizing the technical idea. In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all of them or only those effects, the scope of the present invention should not be understood as being limited thereto.

Meanwhile, the meanings of terms described in the present invention should be understood as follows.

Terms such as "first" and "second" are used to distinguish one component from other components, and the scope of rights is not limited by these terms. For example, a first component may be referred to as a second component, and similarly, a second component may be referred to as a first component.

When a component is referred to as being "connected" to another component, it should be understood that although it may be directly connected to the other component, another component may exist in the middle. On the other hand, when it is mentioned that a component is "directly connected" to another component, it should be understood that there is no other component in the middle. On the other hand, other expressions describing the relationship between the constituent elements, that is, "between" and "just between" or "neighboring to" and "directly neighboring to" should be interpreted as well.

Singular expressions are to be understood as including plural expressions unless the context clearly indicates otherwise, and terms such as "comprises" or "have" refer to specified features, numbers, steps, actions, components, parts, or It is to be understood that it is intended to designate that a combination exists and does not preclude the presence or addition of one or more other features or numbers, steps, actions, components, parts, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the field to which the present invention belongs, unless otherwise defined. Terms defined in a commonly used dictionary should be interpreted as having the meaning of the related technology in context, and cannot be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present invention.

Now, a method for preparing a cosmetic composition for improving acne according to an embodiment of the present invention and a cosmetic composition for improving acne prepared thereby will be described in detail with reference to the drawings.

1 is a flow chart illustrating a method of manufacturing a cosmetic composition for improving acne according to an embodiment of the present invention.

Referring to FIG. 1, first, a natural plant complex is prepared by mixing Schisandra chinensis, figs, mistletoe, camphor ginseng, hawthorn, fat and pine needles (S100).

In the above-described step S100, 4 to 22% by weight of Schisandra, 8 to 11% by weight of figs, 8 to 11% by weight of mistletoe, 14 to 17% by weight of camphor ginseng, 16 to 19% by weight of hawthorn, 16 to 19% by weight of fat, pine needles It is possible to prepare a natural plant complex by mixing 16 to 19% by weight.

Schisandra chinensis is the fruit of the Schisandra chinensis, almost round, ripens in red in August-September, contains 1-2 seeds, has strong acidity and is medicinal. Types include Schisandra (North Schisandra), South Schisandra, and Black Schisandra. Schisandra chinensis mainly grows in the Taebaeksan area, south schisandra chinensis in the southern islands, and black schisandra chinensis in Jeju Island. It is produced in Korea, Japan, Sakhalin Island, and China. Essential oils schizandrin and gomisin A are the main components, and other components such as fatty acid sterol, organic acid, citric acid, vitamin C, etc. are used to strengthen the heart, lower blood pressure, and increase immunity. It strengthens lung function and has an expectorant effect, so it is effective for coughing and thirst. There are about 40 lignans contained in Omija seeds as physiologically active substances, and cancer cell death, cancer metastasis inhibition, antioxidant, liver protection, kidney toxicity inhibition, and antidepressant effects have been reported.

Figs are the fruits of the fig tree, usually round or conical, in various colors such as green, brown, and black. Because of its strong sweetness, it is eaten raw, dried or processed and used as a cooking ingredient. As an alkaline food, it is also used as a medicine in the private sector. In Korea, more than 90% of total domestic production is produced in Yeongam-gun, Jeollanam-do. Fruits have a round shape, flat and round shape, and cone shape. The color of the peel of the fruit is also diverse, such as green, yellowish green, yellow, reddish green, purplish brown, and purplish black. In Europe and the United States, it is often eaten as dried fruit, and in Korea and Japan, it is eaten raw. Varieties are divided into four lines: Carpri, Smyruna, Sanpedro, and Common, and are grown mainly in Europe and America. In Korea, regular and San Pedro types are cultivated, and the main ingredient contains about 10% sugar (glucose and fructose), so the sweet taste is strong. Organic acids include malic acid and citric acid, as well as benzaldehyde, which is effective in treating cancer, and ficin, a protease. In addition, it is rich in enzymes such as lipase, amylase, and oxidase, as well as fiber and protein. Also, as an alkaline food, in ancient Egypt, Rome, and Israel, it was used as a tonic or as a medicine to treat cancer and liver disease. In the private sector, it is used as medicine for indigestion, constipation, diarrhea, hemoptysis, neuralgia, skin disease, anemia, and women's disease, and in the main production area, raw juice is used to treat hemorrhoids and warts. In addition, figs are rich in antioxidants such as polyphenols, which help anti-inflammatory and anti-diabetes, remove triglycerides, and help prevent skin aging.

Mistletoe is a semi-parasitic plant (hemipatasote) with both leaves and stems dark green, branches continually splitting into two branches, and parasitic in domestic oak, hackberry, mulberry, cherry, and birch. The flower is yellow in March and blooms at the end of the branch, and there is no flower stalk, and the small bracts are plate-shaped and male and female. Hwapi(花被) is bell-shaped, has four branches, and the fruit is round and ripens in light yellow in October. It has been reported that lectin, amyrin, inositol, viscotoxin, viscine, flavonoid, polysaccharide, tyramine, histamine, and choline are components of mistletoe. These components are known to have pharmacological effects such as lowering cholesterol, lowering blood pressure, diuretic, antibacterial and antiviral effects, low back pain, postpartum, frostbite. In particular, lectin, the main component of mistletoe, has cytotoxic effects, immunity enhancing effects, and anticancer activity.

Camphor ginseng is also called camphor or jangno ginseng (長腦山蔘), jangno (長蘆), sanyangsansam. Wild ginseng seeds were sown in the mountains and cultivated in the wild. It is said that camphor ginseng grows in the eczema of birch or lacquer trees in the shade deep in the mountains, and it is generally a rare species that does not sprout well even when seeds of wild ginseng are sown. Anti-cancer or anti-diabetic effect Strengthening heart Strengthening liver and gastrointestinal function Stress relief and tonic effect Anemia effect Strengthening concentration Helps improve weak constitution. The anticancer effect of domestic camphor ginseng is excellent. As a result of the so-called survival survey, which was administered to the cancer-affected mice by administration of intestinal ginseng, red ginseng, and ginseng respectively, and the percentage of the time that the mice can survive, the so-called survival survey showed that camphor ginseng was 115% and red ginseng 11%, and ginseng had almost no effect. It was confirmed to be absent. The anticancer effect of camphor ginseng was also confirmed in chemotherapy in clinical trials in patients. It has been shown to inhibit cancer cell growth and alleviate side effects from chemotherapy such as vomiting and muscle pain.

Hawthorn, Crataegus pinnatifida var., a deciduous tree of the genus Malus of the Rosaceae family. typica, Crataegus pinnatifida var. It is a fruit of major, Crataegus cuneata, (Sansa), and the name hawthorn is named because the fruit tastes like an apple and its color is red, so it resembles a small apple. Fruits are spherical or pear-like, 2.5 cm in diameter, and the surface is magenta and glossy, with small gray-white spots. The outer surface is yellowish brown or grayish reddish brown with many fine wrinkles. It is usually cut to a thickness of 3 to 5 cm and distributed. The cross section is constricted and is not flat. It is magenta or light brown, and has 5 to 6 seeds, the color of which is pink. It has a peculiar smell, sour taste, and a little warm in nature. Dried flower apple fruit in oriental medicine is used for dry stomach and indigestion, loss of appetite, acid deficiency or hyperacidity, and is used as medicine for diarrhea, menstrual pain, frostbite, stomach, low back pain, and intestinal bleeding. In the private sector, when cooking meat, it was also used as a meat tenderizer to soften and soften the meat, and fruit juice was used to relieve hangovers. Drinking with a flower apple has the effect of healing fatigue, increasing appetite, nervousness, insomnia, and constipation.

The fruit family is the family of Citrus aurantium L. Poncirus trifoliata Rafin., an evergreen small tree belonging to the Rutaceae family. Immature fruits are harvested from May to June, split horizontally into two, and then rebuilt Dry at low temperature. The components of fat include essential oils such as limonene, linalool, and camphene, and pravos such as poncirin, naringin, hesperidin, and neohesperidin Noids and coumarins such as umbelliferone, auraptene, and imperatorin have been reported. Fonkyrin is known to have antiplatelet and intestinal bacteria inhibitory and anti-helicobacter pylori activity, and naringin has been reported to have cholesterol inhibitory effects, leukemia cells into normal cells, and breast cancer cell proliferation. In addition, it has been reported that the water extract of lipids stabilizes the membrane of mast cells in the peritoneal cavity in the anaphylaxis model induced by compound 48/80 to prevent the death of mice due to anaphylactic shock. It is also rich in vitamins. hives. It is effective in improving flower dust allergy, skin inflammation, atopy, allergic skin, and anti-inflammatory.

Pine needles are leaves and spices of pine trees, an evergreen coniferous tree. They are green and have a thin and elongated shape like needles, and the aroma is refreshing and bitter. In China, it is said to be a food eaten by 仙人. In medical literature, it is a leaf that makes hair grow, makes the intestines comfortable, and makes longevity, and pine needles are also called pine needles. Red pine is used as the best medicinal material in Korea. It has thin leaves, reddish brown stems and no poison. Among pine needles, chlorophyll is used for wound healing, anemia, and stomach ulcers. In addition, the component of glycoginine is effective for diabetes because it has a blood sugar-lowering effect, and it is also good for anemia because it is rich in iron. Pine needles contain a large amount of various minerals and vitamins, which are good nutrients for our body, including beta-carotene that prevents aging and cancer. In particular, a substance called tetpentin (turpentine), which is the cause of the four seasons of blue light in pine needles, contains a lot of unsaturated fatty acids, which prevents accumulation of cholesterol and prevents arteriosclerosis. In addition, by expanding peripheral blood vessels and promoting blood circulation, it improves brain cell function and helps secretion of hormones, helping to balance the body.

In the above-described step S100, when the mixing ratio of the natural plant complex is large, the antioxidant, antibacterial, anti-inflammatory and acne treatment effects derived from the active ingredients of each natural plant may not be properly implemented. In consideration of the product quality, the mixing ratio can be properly controlled.

The natural plant complex extract is extracted from the natural plant complex prepared in step S100 described above using a solvent (S200).

In the above-described step S200, the natural plant complex extract may be extracted by repeating the process of separating the supernatant and the precipitate by immersing the natural plant complex in an ethanol solvent at room temperature for 24 hours for 24 hours. At this time, the ethanol solvent is preferably 10 times the weight of the natural plant complex, and if the concentration of ethanol is more than 70%, an appropriate yield may not be realized, and if the concentration is less than 40%, the active ingredient showing the acne treatment effect is sufficient. Since it may not be extracted, the concentration of ethanol can be adjusted within the range of 40 to 70%.

In the above-described step S200, the process of separating the supernatant and the precipitate by immersing the natural plant complex in purified water 10 times the weight of the natural plant complex and extracting it under reflux for 3 hours at a temperature of about 80 to 90°C is repeated three times. Natural plant complex extracts can be extracted.

In the above-described step S200, the natural plant complex extract may be obtained by using not only the solvents of ethanol and purified water described above, but also other extraction solvents to obtain an extract exhibiting substantially the same effect.

The cosmetic composition for improving acne prepared through the above steps is prepared by using a natural plant complex that is a mixture of schizandra, fig, mistletoe, camphor ginseng, hawthorn, fat, and pine needles, and thus antioxidant, anti-inflammatory, and antibacterial contained in each natural plant. Through the synergistic action of the ingredients, it not only has excellent functions for the treatment and prevention of acne, but also has little irritation to the skin and has excellent feeling of use, moisturizing power, absorption power, and stability.

2 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a second embodiment of the present invention.

2, after the above-described step S200, the natural plant complex extract is filtered and concentrated, and then freeze-dried (S300).

In the above-described step S300, the natural plant complex extract is extracted in the above-described step S200, and after concentrating with a vacuum concentrator within a temperature of 20 ~ 30 ℃ freeze-dried to prepare a solid.

3 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a third embodiment of the present invention.

Referring to Figure 3, after the above-described step S200, mandelic acid (Mandelic Acid), lactobionic acid (Lactobionic Acid), sodium PCA (Sodium PCA), gluconolactone (Gluconolactone), pyruvic acid in the extracted natural plant complex extract. (Pyruvic Acid), Triethanolamine, Sodium Hydroxide, Peg-60 Hydrogenated Castor Oil, Rosemary Essntial Oil, Salicylic Acid ), propylene glycol (Propylene Glycol), 1,2-hexanediol (1,2-Hexanediol) is mixed with one or more compounds selected from the group consisting of purified water (Deionized Water) (S400).

In the above-described step S400, mandelic acid (Mandelic Acid) 1% by weight, lactobionic acid (Lactobionic Acid) 5% by weight, sodium PCA (Sodium PCA) 5% by weight, gluconolactone (Gluconolactone) 5% by weight, pyruvic acid (Pyruvic Acid) 40% by weight, Triethanolamine 0.5% by weight, Sodium Hydroxide 6% by weight, Peg-60 Hydrogenated Castor oil 1% by weight, Rosemary Rosemary Essntial Oil 0.2 wt%, Salicylic Acid 0.2 wt%, Propylene Glycol 6 wt%, 1,2-hexanediol 1,2-Hexanediol 1 wt%, Purified Water (Deionized Water) can be prepared by blending 27.1% by weight and 2% by weight of natural plant complex extract.

In the above-described step S400, after dissolving the compound by dividing the aqueous phase and the oil phase by heating, mixing and cooling using a Homo Mixer Mark II, etc., and mixing by adding flavoring and natural plant complex extract It can be prepared into an essence formulation through a degassing process.

A clinical experiment on the cosmetic composition for improving acne prepared according to the third embodiment as described above was conducted as follows.

First, the clinical trial was conducted on 20 healthy men and women (16 women, 4 men) between their 20s and 50s, but it was confirmed that no other products such as steroids or antibiotics were used from 2 weeks before the start of the experiment to the end of the experiment. I did.

And to all test subjects, a certain amount (2 mg/cm2) of a cosmetic composition for improving acne was applied to the skin area and evaluated after use for 7 days, and the results are shown in Table 1 below. The evaluation below used a 7-point scale method, and the higher the score, the better.

division Average score Feeling 4.86 Moisturizing power 5.11 absorptivity 5.02 Skin irritation 4.25 Acne treatment efficacy and 5.56 Smell 2.44

Referring to [Table 1], it was found that the cosmetic composition for improving acne prepared according to the third embodiment has good feeling of use, moisturizing power, absorption power, and skin irritation, and is effective in treating acne, but has a bad smell.

4 is a flowchart illustrating a method of manufacturing a cosmetic composition for improving acne according to a fourth embodiment of the present invention.

Referring to Figure 4, after the above-described step S200, glycerin (Glycerin), 1,2-hexanediol (1,2-Hexanediol), triethanolamine (Triethanolamine), citric acid (Citric acid), Sodium lactate (50%), Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Tocopheryl Acetate, Lemon Essential Oil ( Lemone Essential Oil) is mixed with one or more compounds selected from the group consisting of Deionized Water (S500).

The cosmetic composition for improving acne prepared through this is a translucent liquid formulation, an alkaline product for neutralizing acidic components such as Pyruvic acid, AHAs, BHAs, and PHAs, which have strong keratin dissolution and removal functions, and the essence according to the third embodiment. It can be used as if wiping the entire face after 5 minutes after using the cosmetic composition for improving acne of the formulation.

In the above-described step SS500, glycerin (Glycerin) 5% by weight, 1,2-hexanediol (1,2-Hexanediol) 2% by weight, triethanolamine (Triethanolamine) 0.5% by weight, citric acid (Citric acid) 0.15% by weight, Sodium lactate (50%) 15% by weight, Butylene Glycol 10% by weight, PEG-40 Hydrogenated Castor Oil 1% by weight, Tocopheryl Acetate (Tocopheryl Acetate) 0.1% by weight, lemon essential oil (Lemone Essential Oil) 0.3% by weight, purified water (Deionized Water) 63.95% by weight, it can be prepared by mixing in a ratio of 2% by weight of natural plant complex extract.

A clinical experiment on the cosmetic composition for improving acne prepared according to the fourth embodiment as described above was conducted as follows.

First, the clinical trial was conducted on 20 healthy men and women (16 women, 4 men) between their 20s and 50s, but it was confirmed that no other products such as steroids or antibiotics were used from 2 weeks before the start of the experiment to the end of the experiment. I did.

In addition, to all test subjects, a certain amount (2 mg/cm2) of a cosmetic composition for improving acne was applied to the skin area after washing and evaluated after use for 7 days, and the results are shown in Table 2 below. The evaluation below used a 7-point scale method, and the higher the score, the better.

division Average score Feeling 5.53 Sticky 5.73 Cleaning power 5.53 Skin irritation 5.27 Acne treatment efficacy 4.73 Smell 5.67

Referring to [Table 2], the cosmetic composition for improving acne prepared according to the fourth embodiment was found to have good feeling of use, stickiness, cleansing power, skin irritation, acne treatment efficacy, and scent.

5 is a flow chart illustrating a method of manufacturing a cosmetic composition for improving acne according to a fifth embodiment of the present invention.

Referring to Figure 5, after the above-described step S200, triethanolamine (Triethanolamine), 1,2-hexanediol (1,2-Hexanediol), carbomer (Carbomer), disodium IDTA (Disodium EDTA), Potassium hydroxide (85%), Allantoin, Lactobionic Acid, Gluconolactone, Stebiten fresh, Sodium Hyaluronate ( Sodium hyaluronate), PEG-60 Hydrogenated Castor Oil, Salicylic Acid, Mandelic acid, Tea tree oil, Butylene Glycol Glycol) and one or more compounds selected from the group consisting of liposomes and deionized water are mixed (S600).

The cosmetic composition for acne improvement produced through this is a cream formulation, soothing sensitive skin, prescribing low concentrations of AHAs, BHAs, PHAs and pharmaceutical ingredients, and prescribing a high concentration of natural plant ingredient complex extract that is safe for the skin and applying it to the skin. It can have a lasting acne care effect.

In the above-described step S600, triethanolamine (Triethanolamine) 0.3% by weight, 1,2-hexanediol (1,2-Hexanediol) 4% by weight, Carbomer (Carbomer) 1% by weight, Disodium EDTA (Disodium EDTA) ) 0.02% by weight, potassium hydroxide (85%) 5% by weight, allantoin 0.2% by weight, lactobionic acid 0.1% by weight, gluconolactone 0.1% by weight, stebitene Fresh (Steviten fresh) 0.05% by weight, sodium hyaluronate (Sodium hyaluronate) 0.02% by weight, PEG-60 Hydrogenated Castor Oil (PEG-60 Hydrogenated Castor Oil) 1% by weight, Salicylic Acid (Salicylic Acid) 0.1% by weight %, Mandelic acid 0.1% by weight, Tea tree oil 0.2% by weight, Butylene Glycol 10% by weight, Liposome 5% by weight, Purified water 70.81 It can be prepared by blending in a ratio of 2% by weight of natural plant complex extract.

A clinical experiment on the cosmetic composition for improving acne prepared according to the fifth embodiment as described above was conducted as follows.

First, the clinical trial was conducted on 20 healthy men and women (16 women, 4 men) between their 20s and 50s, but it was confirmed that no other products such as steroids or antibiotics were used from 2 weeks before the start of the experiment to the end of the experiment. I did.

And to all test subjects, a certain amount (2 mg/cm2) of a cosmetic composition for improving acne was applied to the skin area after washing and evaluated after use for 7 days, and the results are shown in Table 3 below. The following evaluation was performed using a 7-point scale, and the higher the score, the better.

division Average score Feeling 6.01 Moisturizing power 5.88 Sticky 5.25 absorptivity 5.75 Skin irritation 5.81 Acne treatment efficacy 5.50 Smell 5.19

Referring to [Table 3], it was found that the cosmetic composition for improving acne prepared according to the fifth embodiment had excellent feeling of use, moisturizing power, stickiness, absorption power, skin irritation, acne treatment efficacy, and scent.

Experimental Example 1. Measurement of antioxidant effect of cosmetic composition for improving acne

1-1. DPPH radical scavenging ability measurement

The antioxidant effect was tested by measuring the DPPH radical scavenging ability of the cosmetic composition for improving acne prepared according to the first embodiment.

To test this, a cosmetic composition for improving acne was used in which the ratio of natural plant components was composed of SP-1, SP-2, SP-3, and SP-4 as shown in Table 4 below.

division SP-1 SP-2 SP-3 SP-4 Schisandra 14 10.5 10.5 10.5 FIG 11 10.5 10.5 10.5 mistletoe 11 10.5 10.5 10.5 Camphor ginseng 16 16 16 16 Mountain temple 16 17.5 16 16 Jisil 16 17.5 19 17.5 pine needles 16 17.5 17.5 19 Total (% by weight) 100 100 100 100

For DPPH electron donating ability (EDA), 1 mL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 mL of each sample solution, stirred and left for 30 minutes, and the absorbance was measured at 517 nm. . The electron donating ability was expressed by the decrease in absorbance of the group with and without the sample solution.

1,1-diphenyl-2-picrylhydrazyl (DPPH), which is widely used for antioxidant effects, has the property of stable free radicals by sharing extra electrons in the molecule. When DPPH solution is mixed with other substances, it becomes purple as the reduction increases. It represents the shape that loses. The antioxidant function can be measured by the ratio of remaining DPPH through the free radical scavenging activity at absorbance of 517 nm. As a result of measuring the DPPH radical scavenging activity of the herbal compound, it was confirmed as shown in FIG. 6. The radical scavenging ability was increased depending on the concentration, and at the concentration of 1,000㎍/ml of the herbal compound, SP-1: 53.8%, SP-2: 69.9%, SP-3: 57.1%, SP-4: 50.4%. It was confirmed that the scavenging ability was the most excellent. This result can be inferred that SP-2 has excellent antioxidant activity given that Vit-C is a single component.

1-2. ABTS radical scavenging ability measurement

For the cosmetic composition for improving acne prepared according to the first embodiment, the antioxidant effect was tested by measuring ABTS radical scavenging activity, which is commonly used, such as DPPH radical scavenging activity.

After preparing 7 mM ABTS buffer and 2.45 mM potassium persulfate buffer (K ₂ S ₂ O ₈ ), they were mixed in a ratio of ABTS: K ₂ S ₂ O ₈ = 2: 1, and then reacted by blocking light for 12 to 16 hours. Dilute the stock buffer so that the OD value measured at 734 nm is 0.70±0.02, add 100 μL of the extract sample diluted by concentration to 100 μL of buffer, react at room temperature for 6 minutes, measure the absorbance at 734 nm, and add no addition with the addition port. It was expressed as the rate of decrease in absorbance of the group.

ABTS radical scavenging activity is a characteristic color of cyan when ABTS+ radicals are generated by the reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and potassium persulfate. Accordingly, it is a method of measuring decolorization to light green, and both hydrogen-donating antioxidant and chain breaking antioxidant can be measured, and the results were confirmed as shown in FIG. 7. Concentration-dependently, ABTS+ radical scavenging activity increased. At 1000㎍/ml concentration of herbal compound, SP-1: 94.3%, SP-2: 99.1%, SP-3: 93.9%, and SP-4: 90.8%. It was confirmed that the radical scavenging ability was the most excellent. In the case of SP-2, vit-C, a positive control, is more excellent than that exhibiting 94.3% ABTS+ radical scavenging activity at a concentration of 1000㎍/ml. Considering that vit-C is a single component, SP-2 is a very good ABTS+ radical. It can be inferred that it has the ability to erase.

1-3. Superoxide anion radical scavenging ability measurement

The antioxidant effect was tested by measuring superoxide anion radical scavenging ability of the cosmetic composition for improving acne prepared according to the first embodiment.

Superoxide anion radical scavenging activity was measured by the nitrobule tetrazolium (NBT) reduction method. To 0.1 mL of each sample solution and 0.4 mL of 0.1 M potassium phosphate buffer (pH 7.5), add 1 mL of a substrate solution dissolved in Xanthine (0.4 mM) and NBT (0.24 mM), and then add 1 mL of xanthine oxidase (0.2 U/mL). After reacting at 37° C. for 20 minutes, the amount of generated superoxide anion radical was measured for absorbance at 560 nm.

NBT (Superoxide anion radical) scavenging ability is a method of confirming superoxide anion radical scavenging ability with nitro blue tetrazolium by reacting the superoxide anion radical generated in the process of producing uric acid by xanthine oxidase using xanthine as a substrate with a sample. The effect of inhibiting the generation of radicals generated by was confirmed as shown in FIG. 8. Concentration-dependently increased NBT radical scavenging ability, and at 1,000㎍/ml concentration of herbal compound, SP-1: 11.0%, SP-2: 54.9%, SP-3: 35.4%, SP-4: 19.6% were superoxide in SP-2. It was confirmed that the anion radical scavenging ability was the best. SP-2 showed a radical scavenging activity of 35.2% at a low concentration of 100㎍/ml, and this result was less radical scavenging activity than the NBT radical scavenging activity of vit-C 55.4%, a positive control at the same concentration, but vit-C was a single component Considered, it can be inferred that HC-2 has excellent NBT radical scavenging ability.

Experimental Example 2. Measurement of antibacterial effect of cosmetic composition for improving acne

2-1. Antibacterial activity measurement

Measured by using the paper disk method, 1 platinum was taken from each strain cultured on the plate medium and incubated for 18 to 24 hours in 10 mL of the liquid medium to activate, and then 0.1 mL of the bacterial solution was inoculated into 10 mL of the liquid medium to 3 to 6 After cultivation for time, inoculation was carried out so that the number of bacteria per plate medium was about 1×107 cells, and uniformly smeared with a sterile cotton swab. After placing a sterilized filter paper disc on a solid plate, absorb the sample by concentration so that it becomes 0.05mL/disc, incubate at 37℃ for 18~24 hours, and measure the diameter of the clear zone (mm) around the disk.

Pseudomonas aeruginosa (Pa), Escherichia coli (EC), and Staphylococcus aureus (Sa) have been reported as bacteria that can induce skin inflammation among the skin flora, and Propionibacterium acnes (P.acne) as the bacteria that cause skin acne ) Is known. As a result of confirming the growth inhibition ring (Clear zone) in the cosmetic composition for improving acne, the development of natural products-derived skin acne prevention, alleviation, and treatment, and the antibacterial effect of bacteria that may cause inflammation on the skin among the skin flora 9, SP-2 is shown in Figure 10, SP-3 is shown in Figure 11, SP-4 is as shown in Figure 12, SP-1, SP-2, SP-3, SP-4 all clear in Pa, Ec bacteria The zone was confirmed, but the clear zone could not be confirmed in Sa, Ca, and An bacteria. In Pa bacteria, SP-1 was found to have the best antimicrobial activity in Ec bacteria. As a result of measuring the antibacterial activity against P.acne bacteria, the antibacterial effect was confirmed in SP-1 and SP-2, but the antibacterial activity was not confirmed in SP-3 and SP-4.

Experimental Example 3. Measurement of anti-inflammatory effect of cosmetic composition for improving acne

3-1. Cell viability measurement

The anti-inflammatory effect was tested by measuring the cell viability of the cosmetic composition for improving acne prepared according to the first embodiment.

The survival rate of macrophages (Raw 264.7) of cosmetic compositions for improving acne (SP-1, SP-2, SP-3, SP-4) was determined by MTT assay to reduce MTT, which is converted into a purplish formazan product by mitochondrial dehydrogenase. Measured. As a result of the experiment, as shown in FIG. 13, a survival rate of 80.2% or more was confirmed for all of SP-1, SP-2, SP-3, and SP-4 to a concentration of 0.1 to 1000 μg/mL. Among SP-1, SP-2, SP-3, and SP-4, SP-1 was found to have a high cell viability of 101.95 at a concentration of 1000 μg/mL.

3-2. Nitric oxide inhibition ability measurement

The anti-inflammatory effect was tested by measuring the nitric oxide inhibitory ability of the cosmetic composition for improving acne prepared according to the first embodiment.

NO (Nitric Oxide) is one of the free radicals and is known to intensify inflammation by promoting inflammatory reactions such as vascular permeability and edema in an inflammatory state, as well as promoting biosynthesis of inflammatory mediators. L-arginine, a substrate of NO (Nitric Oxide), turns into L-citrulline and nitrogen monoxide, which quickly turns into stable nitrogen dioxide, nitrite, and nitrate. The Griess reagent chemically reacts with nitrite to form a purple azo salt, which is consistent with the concentration of nitrogen monoxide. Therefore, the NO (Nitric Oxide) production ability was measured in the form of NO _2- present in the cell culture medium. Treated the cosmetic composition (SP-1, SP-2, SP-3, SP-4) for acne improvement (SP-1, SP-2, SP-3, SP-4) to LPS-induced Raw 264.7 Cells at a concentration of 0.1, 1, 10, 100, 1000 μg/mL, As a result of measuring the amount of NO (Nitric Oxide), as shown in FIG. 14, it was confirmed that 44.5% and 39.79% of NO (Nitric Oxide) production inhibitory ability in SP-2 and SP-4 samples 100 μg/mL was confirmed, and 1000 At μg/mL concentration, it was confirmed that NO (Nitric Oxide) production was significantly inhibited as it was concentration-dependent and significantly inhibited NO (Nitric Oxide) inhibitory activity of 56.42% and 46.94%, respectively.

3-3. Measurement of pro-inflammatory cytokine secretion

The anti-inflammatory effect was tested by measuring the secretion of Pro-inflammatory cytokine for the cosmetic composition for improving acne prepared according to the first embodiment.

Macrophage-mediated PGE ₂ , like NO (Nitric Oxide), is an important inflammatory mediator mainly involved in the transmission of pain and fever in damaged areas or tissues, and is produced by COX-2 during inflammatory reactions, and in a small amount, essential for biometabolism. However, excessive PGE ₂ caused by overexpression of COX-2 is a powerful inflammatory mediator, expanding blood vessels and increasing vascular permeability, thereby increasing the chemotaxis of leukocytes to the inflammatory site. As a result of confirming the effect of inhibiting the production of PGE _{2 in} the cosmetic compositions for improving acne (SP-1, SP-2, SP-3, SP-4), 48.82% at 1000 μg/mL of the SP-2 sample as shown in FIG. 15 The high PGE ₂ production inhibitory ability was confirmed, and also in SP-1 and SP-4, 27.55% and 26.94% of PGE ₂ production inhibitory ability were confirmed. In the inhibition of NO (Nitric Oxide) production, it was confirmed that SP-2 among SP-1, SP-2, SP-3, and SP-4 samples was significantly inhibited like PGE ₂ production inhibitory ability.

As described above, the embodiments of the present invention are not implemented only through the above-described apparatus and/or operation method, but through a program for realizing a function corresponding to the configuration of the embodiment of the present invention, a recording medium in which the program is recorded, etc. It may be implemented, and this implementation can be easily implemented by an expert in the technical field to which the present invention belongs from the description of the above-described embodiment.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

S100: manufacturing step
S200: extraction step
S300: drying step
S400: first mixing step
S500: 2nd mixing step
S600: 3rd mixing step

Claims (5)

A manufacturing step of preparing a natural plant complex by mixing Schisandra chinensis, figs, mistletoe, camphor ginseng, hawthorn, branch, and pine needles; And
Including an extraction step of extracting the natural plant complex extract from the natural plant complex using a solvent,
The manufacturing step,
Schisandra chinensis 4 to 22% by weight, figs 8 to 11% by weight, mistletoe 8 to 11% by weight, camphor ginseng 14 to 17% by weight, hawthorn 16 to 19% by weight, fat 16 to 19% by weight, pine needles 16 to 19% by weight are mixed To manufacture natural plant complexes,
After the extraction step,
Triethanolamine 0.3% by weight, 1,2-hexanediol (1,2-Hexanediol) 4% by weight, Carbomer 1% by weight, Disodium EDTA 0.02% by weight, potassium hydroxide (Potassium hydroxide (85%)) 5% by weight, Allantoin 0.2% by weight, Lactobionic Acid 0.1% by weight, Gluconolactone 0.1% by weight, Stebiten fresh 0.05% by weight %, Sodium hyaluronate 0.02% by weight, PEG-60 Hydrogenated Castor Oil 1% by weight, Salicylic Acid 0.1% by weight, Mandelic acid ) 0.1 wt%, Tea tree oil 0.2 wt%, Butylene Glycol 10 wt%, Liposome 5 wt%, Deionized Water 70.81 wt%, The natural plant complex A method for producing a cosmetic composition for improving acne, characterized in that prepared by blending in a proportion of 2% by weight of the extract.
delete The method of claim 1, wherein the extraction step,
A method for preparing a cosmetic composition for improving acne, characterized in that the natural plant complex extract is extracted by repeating the process of immersing the natural plant complex in the solvent at room temperature for 24 hours three times.
The method of claim 3, wherein after the extraction step,
A method for producing a cosmetic composition for improving acne, further comprising a drying step of lyophilizing after filtering and concentrating the natural plant complex extract.
A cosmetic composition for improving acne prepared by the method for preparing a cosmetic composition for improving acne according to any one of claims 1, 3, and 4.

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